CN105524824A - Photo-bioreactor - Google Patents
Photo-bioreactor Download PDFInfo
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- CN105524824A CN105524824A CN201410596446.5A CN201410596446A CN105524824A CN 105524824 A CN105524824 A CN 105524824A CN 201410596446 A CN201410596446 A CN 201410596446A CN 105524824 A CN105524824 A CN 105524824A
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- reaction vessel
- bioreactor
- light source
- organism reaction
- transparent organism
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Abstract
The invention discloses a photo-bioreactor, aiming at providing a small-scale photo-bioreactor which is simple to manufacture, low in cost and high in microalgae culture efficiency. A transparent bio-reaction container is arranged on a base; an air vent in the upper end of the transparent bio-reaction container is sealed with outside and is provided with a ventilating pipe; the ventilating pipe is sequentially connected to an air filter, a tee regulating valve and an air compressor; an exhaust port is formed in the side face of the transparent bio-reaction container; the exhaust port is sealed with the outside and is provided with an exhaust pipe; a bacterium filtering membrane or a filtering medium is arranged at the tail end of the interior of the exhaust pipe; and a plurality of light sources are arranged around the transparent bio-reaction container on the base. The photo-bioreactor, by virtue of the air compressor and the filter, can achieve the preparation of pressurized sterile air and the ventilation quantity of the sterile air entering the reactor can be regulated by virtue of the tee regulating valve, so that the concentration of dissolved carbon dioxide and stirring degree of a microalgae culture system are effectively regulated and controlled artificially, and the uniform and consistent nutrition distribution inside the system is guaranteed; and the photo-bioreactor is simple to manufacture and is low in cost.
Description
Technical field
The present invention relates to a kind of microdisk electrode optical-biological reaction apparatus.
Background technology
Bioreactor is the gordian technique of micro-algae high-density culture.Bioreactor for microdisk electrode mainly contains mechanical agitation type bioreactor and tubular light bio-reactor etc.At present, in micro-algae on a small scale culture vessel, be generally mechanical agitation type bioreactor.The shearing injury of this reactor stirring arm to cell is large, and light source utilization rate is not high, and microdisk electrode density is low.And its price is generally somewhat expensive, add the burden of experiment.
Summary of the invention
The present invention is to overcome weak point of the prior art, provides a kind of and makes simple, cheap, that microdisk electrode efficiency is high small-scale bioreactor.
The present invention is achieved through the following technical solutions:
A kind of bioreactor, it is characterized in that, comprise base, transparent organism reaction vessel, light source, air filter, three-way control valve and air compressor, described base is provided with transparent organism reaction vessel, the ventage of described transparent organism reaction vessel upper end seals with extraneous and ventpipe is housed, described ventpipe successively with air filter, three-way control valve, air compressor connects, described transparent organism reaction vessel side is provided with venting port, described venting port seals with extraneous and vapor pipe is housed, in the vapor pipe inside end of transparent organism reaction vessel outside, bacteriological filtration film or filtration medium are housed, described base is surrounded with multiple light source around transparent organism reaction vessel.
Described transparent organism reaction vessel is tapered.
The side of described transparent organism reaction vessel is provided with light source retaining plate, and described light source retaining plate is fixed with light source.
The distance of the lower port distance transparent biological reaction container bottom of described ventpipe is 3-5cm.
On described base, transparent organism reaction vessel surrounding respectively has a light source, and side light source retaining plate is provided with two light sources.
Two mouths of described three-way control valve are connected with air compressor and air filter respectively, and the 3rd mouth communicates with the external world.
The present invention has following technique effect:
1. bioreactor of the present invention achieves the preparation of pressurized sterile air by air compressor and strainer, three-way control valve is utilized to regulate sterile air to enter the air flow of reactor, thus effectively realize concentration, the stirring extent of the molten carbonic acid gas of artificial adjustment microdisk electrode system, the inner nutrient distribution uniformity of the system that ensured, gaseous interchange fully and asepticize cultivation.Meanwhile, owing to using the method passing into gas to stir, make cell be subject to less shear property model, be conducive to the growth of micro-algae.
2. around reaction vessel, be provided with light source, effectively provide the light intensity and light quality that micro algae growth needs, substantially increase the growth velocity of micro-algae.
3. whole reactive system makes simple, cheap, convenient, flexible.Meanwhile, operationally, the links such as sterilizing, inoculation, sampling, cleaning and dismounting are all very convenient for this reactor.
Accompanying drawing explanation
Fig. 1 is bioreactor ground structure schematic diagram of the present invention.
In figure: 1. light source 2. light source retaining plate 3. transparent organism reaction vessel 4. ventpipe 5. vapor pipe 6. air filter 7. three-way control valve 8. air compressor 9. base.
Embodiment
Below in conjunction with the drawings and specific embodiments to the detailed description of the invention.
Fig. 1 is the structural representation of bioreactor of the present invention, comprise base 9, transparent organism reaction vessel 3, light source 1, air filter 6, three-way control valve 7 and air compressor 8, base is equipped with transparent organism reaction vessel 3, the ventage of transparent organism reaction vessel upper end seals with extraneous and ventpipe 4 is housed, the distance of the lower port distance transparent biological reaction container bottom of ventpipe is 3-5cm, to make the sterile air passed into have better stirring action.Ventpipe 4 is connected with air filter 6, three-way control valve 7 and air compressor 8 successively, during connection, two mouths of three-way control valve are connected with air compressor and air filter respectively, 3rd mouth communicates with the external world, by regulating three-way control valve, change the air capacity entering transparent organism reaction vessel.Transparent organism reaction vessel side is provided with venting port, the diameter of venting port is generally 5cm, venting port seals with extraneous and vapor pipe 5 is housed, at the vapor pipe 5 inside end place of transparent organism reaction vessel outside, bacteriological filtration film or filtration medium are housed, filtration medium can select cotton, to ensure the sterile culture of micro-algae.Base is surrounded with multiple light source 1 around transparent organism reaction vessel, whole reaction system is placed on homo(io)thermism and in regulatable greenhouse, thinks the temperature that the growth of micro-algae provides suitable.
During light source design, on base, transparent organism reaction vessel surrounding respectively has a light source, and transparent organism reaction vessel side arranges light source retaining plate 2, and described light source retaining plate is fixed with light source.Side light source can have two, effectively to provide light intensity and light quality.Transparent organism reaction vessel can select glass filter flask.
During use, glass filter flask, ventpipe, air filter, vapor pipe and bacteriological filtration film thereof or filtration medium are wrapped sterilizing, and aseptically add fresh culture and micro-algae algae kind successively, carry out the sterile culture of micro-algae.Isochrysis galbana is being cultivated with this bioreactor in this laboratory, and culture condition is as follows: intensity of illumination (3550lx), ventilates every day three times, each ventilation 10 minutes.Within seven days, can reach the harvesting time of micro-algae, cell concn is 2.73 × 10
7individual/ml, dry cell weight can reach 0.66g/L.
Bioreactor of the present invention is prepared by transparent reaction container, light source and sterile air and delivery system three part forms, and three parts can freely separate and combine.The Lights section can be selected to be made up of switch and fluorescent tube, and light source is looped around around reaction vessel, is fixed on base and side, and quantity, the position of light source can freely change, and light intensity therefore can free adjustment, and can time manual control photoperiod.Sterile air preparation and delivery system can provide enough carbon sources for micro-algae, play the effect of stirring, and cell shearing power are less simultaneously, are beneficial to the growth of micro-algae.
Claims (6)
1. a bioreactor, it is characterized in that, comprise base, transparent organism reaction vessel, first source, air filter, three-way control valve and air compressor, described base is provided with transparent organism reaction vessel, the ventage of described transparent organism reaction vessel upper end seals with extraneous and ventpipe is housed, described ventpipe successively with air filter, three-way control valve, air compressor connects, described transparent organism reaction vessel side is provided with venting port, described venting port seals with extraneous and vapor pipe is housed, in the vapor pipe inside end of transparent organism reaction vessel outside, bacteriological filtration film or filtration medium are housed, described base is surrounded with multiple light source around transparent organism reaction vessel.
2. bioreactor according to claim 1, is characterized in that, described transparent organism reaction vessel is tapered.
3. bioreactor according to claim 1 and 2, is characterized in that, the side of described transparent organism reaction vessel is provided with light source retaining plate, and described light source retaining plate is fixed with light source.
4. bioreactor according to claim 1, is characterized in that, the distance of the lower port distance transparent biological reaction container bottom of described ventpipe is 3-5cm.
5. bioreactor according to claim 3, is characterized in that, on described base, transparent organism reaction vessel surrounding respectively has a light source, and side light source retaining plate is provided with two light sources.
6. bioreactor according to claim 3, is characterized in that, two mouths of described three-way control valve are connected with air compressor and air filter respectively, and the 3rd mouth communicates with the external world.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410596446.5A CN105524824A (en) | 2014-10-21 | 2014-10-21 | Photo-bioreactor |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410596446.5A CN105524824A (en) | 2014-10-21 | 2014-10-21 | Photo-bioreactor |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105524824A true CN105524824A (en) | 2016-04-27 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410596446.5A Pending CN105524824A (en) | 2014-10-21 | 2014-10-21 | Photo-bioreactor |
Country Status (1)
| Country | Link |
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| CN (1) | CN105524824A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106434279A (en) * | 2016-09-08 | 2017-02-22 | 天津现代职业技术学院 | Photosynthetic bacteria concentration device |
| CN108300646A (en) * | 2018-03-27 | 2018-07-20 | 华南理工大学 | A kind of at the uniform velocity sterile air-breather |
-
2014
- 2014-10-21 CN CN201410596446.5A patent/CN105524824A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106434279A (en) * | 2016-09-08 | 2017-02-22 | 天津现代职业技术学院 | Photosynthetic bacteria concentration device |
| CN108300646A (en) * | 2018-03-27 | 2018-07-20 | 华南理工大学 | A kind of at the uniform velocity sterile air-breather |
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Application publication date: 20160427 |