CN101760429B - Simple and easy cylindrical reaction vessel for microalgae culture - Google Patents

Simple and easy cylindrical reaction vessel for microalgae culture Download PDF

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Publication number
CN101760429B
CN101760429B CN 200810240025 CN200810240025A CN101760429B CN 101760429 B CN101760429 B CN 101760429B CN 200810240025 CN200810240025 CN 200810240025 CN 200810240025 A CN200810240025 A CN 200810240025A CN 101760429 B CN101760429 B CN 101760429B
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reaction vessel
microalgae
air
breather
gas
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CN101760429A (en
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石悦
耿金峰
张晋阳
王慧岭
李静
刘敏胜
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ENN Science and Technology Development Co Ltd
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ENN Science and Technology Development Co Ltd
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00Bioreactors or fermenters specially adapted for specific uses
    • C12M21/02Photobioreactors
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00Means for mixing, agitating or circulating fluids in the vessel
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/06Nozzles; Sprayers; Spargers; Diffusers
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/20Degassing; Venting; Bubble traps
    • C12M29/22Oxygen discharge

Abstract

The invention discloses a simple and easy cylindrical reaction vessel for microalgae culture, which comprises a photobiological reaction system, a ventilation device and a detection device, wherein the detection device consists of an air head, an air pipe and a three-way valve, the air is uniformly distributed, and formed air bubbles are small and are favorable for the growth of the microalgae. The ventilation device has good gas-liquid mixing effect. On one hand, the ventilation device can provide sufficient carbon dioxide for the growth of the microalgae and is favorable for the oxygen exhaust, and on the other hand, sufficient light illumination can be received in the microalgae growth process. A filter unit is arranged in an air inlet pipeline. The photobiological reaction vessel can realize the effects that the culture liquid obtains uniform and sufficient light illumination through regulating the culture liquid flowing by the air flowing and the cutting force is small. The surface of the cylinder can receive the light, the sun light can be used as a light source, the culture conditions in the reaction vessel can be easily observed, the reaction vessel can be easily cleaned, the manufacture is easy, the cost is low, and the simple and easy cylindrical reaction vessel is suitable for culturing the microalgae.

Description

A kind of simple and easy cylindrical reaction vessel for microalgae culture
Technical field
The present invention relates to the optical-biological reaction field, the breather of particularly a kind of bioreactor and bioreactor.
Background technology
Little algae can effectively utilize CO 2, luminous energy and inorganic salts synthetic protein, fat, glucide and multiple high added value biologically active substance, can produce protective foods, foodstuff additive, feed, bio-feritlizer, makeup and other natural product through culturing microalgae.Therefore development and development of new efficiently the high-density culture of bioreactor and little algae receive increasing concern.
At present, little algae is cultivated and mainly is divided into open and closed two kinds of bioreactors.Because it is easy to control that closed reactor has culture condition, but aseptic technique is prone to carry out advantages such as high-density culture, become the direction of bioreactor development.Closed photo bioreactor mainly comprises tubular type, flat and these three kinds of forms of pillar.
But all there is certain defective in closed reactor.The dissolved oxygen level of tubular reactor is easy to reach 200%, and the accumulation of dissolved oxygen can suppress the growth of algae.Plate-type reactor receives the restriction of height, generally is not higher than 1.5m, and can forms the dead angle in the bottom of reactor drum, is unfavorable for the growth of little algae.Column reactor has overcome the restriction of oxygen accumulation, and simple in structure, nutrient solution mixes, and can obtain competent light source.
In recent years; Structure is simple, single cost is low because airlift reactor has, be prone to clean maintenance, nutrient solution flows the heat and mass transfer coefficient regular, that energy consumption is low and higher and amplify characteristics such as easy, and such reactor drum has obtained bigger application aspect biochemical industry; People (Miyamoto K such as Miyamoto in 1988; WableO; Benemann J R.Vertical tubular reactor for microalgae cultivation.Biotechnol.Lett.; 1988,10,703-708) utilize column reactor to obtain about 0.6g l at outdoor cultivation Nostoc (beads algae) and Anacystis (Anacystis nidulans) -1d -1Unit volume productivity.Hu in 1994 and Richmond (Hu; Q.&Richmond, A.Optimizing the population density in Isochrysis galbana grownoutdoors in a glass column photobioreactor.J.Appl.Phycol., 1994; 6; 391-96) use 1.5m high, the similar system that the column reactor of 2.6cmID is formed, outdoor cultivation Isochrysis galbana (chrysophyceae) has obtained 1.6gl -1d -1Unit volume productivity.Column reactor has the nutrient solution good mixing effect, carbonic acid gas utilization ratio advantages of higher.(ocean science, 2000,5 (24): 14-16) utilize the pillar bioreactor to cultivate chlorella, average daily production 0.2134gl such as Zhang Xu -1d -1
The breather of bioreactor is generally gas distributor or foraminous pvc pipe at present.The bubble that gas distributor and pvc pipe produce is inhomogeneous, and along with the extension of sparger and pipe, tolerance is more and more littler, is unfavorable for the growth of little algae.
Summary of the invention
The present invention has overcome the technical shortcoming of pre-existing reactors, provide a kind of simple in structure, that light utilization efficiency is high, be convenient to high-density culture, little algae is gathered is easy, easily-controlled reaction conditions be suitable for the column type bioreactor that little algae is cultivated.Overcome the shortcoming of existing bioreactor breather, provide a kind of gas distribution even, simple in structure, be easy to the bioreactor breather of pilot-gas flow, can be used in all kinds bioreactor.
The present invention realizes through following technical proposals:
A kind of bioreactor is characterized in that, comprises cylindrical optical-biological reaction system, breather, proofing unit.Said optical-biological reaction system is made up of right cylinder, circular top cover and a circular base, and circular top cover can move freely, and inlet mouth, opening for feed and instrument probe mouth are arranged, and liquid outlet is arranged respectively at right cylinder middle part and bottom.Right cylinder and round bottom are bonded through special processing.Usually the glue of the bonding use of synthetic glass is 601, and bonding mode is bonding for directly, if material is a glass, can adhesion be integral direct fusion.Periphery receives light, and sunshine capable of using is easy to cultivation situation in the observing response device as light source, is easy to clean, and makes simply, and is with low cost, is suitable for culturing microalgae.
Described optical-biological reaction system is a column, and material is transparent glass, synthetic glass or resin material etc.
Described breather is characterized in that, the tree-shaped diffuser of being made up of through threeway gas head and tracheae.Gas distribution is even, and the bubble of formation is less, helps micro algae growth.This breather gas-liquid mixed is good, can help the discharge of oxygen for micro algae growth provides enough carbonic acid gas on the one hand; On the other hand, can accept enough illumination in the micro algae growth process.
Described tree-shaped diffuser is divided into two arms by a person in charge through threeway, and two arms can be divided into four through threeway, can obtain the arm that arbitrary number is wanted through threeway, a terminal pipe coupling gas head.
Described tree-shaped diffuser can be fixed on the object of annulus, straight tube and/or random shape that iron wire or other materials make.
Described threeway can be four-way or manifold more.
Described breather also can be used for plate-type reactor, tubular reactor or other all kinds of reactor drums.
Described tree-shaped breather; In the admission passage; The filter membrane and/or the filter core in different apertures can be installed as required at an arbitrary position, and in order to removing impurity and mikrobe in the gas, filter membrane and/or filter core are installed near the vent line air intake valve and/or near the gas head usually.
Said source of the gas is air pump or gas cylinder, utilizes flexible pipe to stretch into the bioreactor bottom through inlet pipe, and the body of ventilating is air, carbonic acid gas or air and carbon dioxide gas mixture.Vent line is equipped with gas meter.Said bioreactor flows through gas flow adjusting nutrient solution and can make nutrient solution obtain even competent illumination, and shearing force is less.
Said proofing unit comprises Temperature Detector, pH detector and dissolved oxygen detector.
A liquid outlet is arranged at said pillar photo-biological reactor bottom, is convenient to gathering of little algae.
The present invention has following technique effect:
1. the present invention adopts air lift type, relies on gas flow to drive the nutrient solution circulation, has avoided the injury of shearing force to little algae.
2. the optical-biological reaction system adopts column, can make the micro algae culturing liquid thorough mixing.
3. the optical-biological reaction system adopts transparent material to help observing the growing state of little algae.
4. opening for feed is arranged at optical-biological reaction system top, is convenient to the discharge of oxygen, has avoided the too high harm to micro algae growth of dissolved oxygen.
5. the instrument probe mouth is arranged at optical-biological reaction system top, is convenient to the physical parameter of nutrient solution in the culturing process is monitored in real time, promptly and accurately grasps the micro algae growth situation.
6. there is liquid outlet at optical-biological reaction system right cylinder middle part, is convenient in airtight reactor drum, take a sample.
7. liquid outlet is arranged at optical-biological reaction system right cylinder bottom, after little algae is cultivated finite concentration, can open the liquid outlet valve and emit algae liquid, adds fresh culture simultaneously at the reactive system top, can realize cultured continuously; When the requirement that achieves the goal of microalgae cell concentration, utilize the settling character of little algae self or add flocculation agent to make little algae be deposited to reactor bottom, through right cylinder bottom liquid outlet nutrient solution is discharged again, collect microalgae cell at last.
8. tree-shaped diffuser, gas distribution is even, and bubble is less, helps the absorption of little algae to carbonic acid gas.
9. tree-shaped diffuser has increased gas-liquid mixed, helps discharging the oxygen that produces in the micro algae growth process.
10. tree-shaped diffuser has increased the mixing of substratum, helps the utilization of little algae to luminous energy.
11. breather is equipped with gas meter and valve, can help the transmission of carbonic acid gas and oxygen through the flow rate of adjustments of gas regulating nutrient solution, the mixing of nutrient solution, the utilization ratio of increase light.
12. vent line is equipped with filtration unit, can remove by filter impurity in the air, mikrobe and protozoon guarantee the pure culture of little algae.
Description of drawings
Fig. 1 is the one-piece construction synoptic diagram of embodiment of the invention bioreactor.
Fig. 2 is the one-piece construction synoptic diagram of the tree-shaped diffuser of the embodiment of the invention.
Fig. 3 is the diffuser synoptic diagram of tying up on annulus, can tie up a circle or many circles.
Fig. 4 is the diffuser synoptic diagram of tying up on straight tube.
Wherein, 1-inlet pipe; The 2-air intake valve; 3-gas millipore filtration; The 4-gas meter; The 5-inlet mouth; 6-instrument probe mouth; The 7-opening for feed; 8-optical-biological reaction system; 9, the 10-liquid outlet; 11-master's tracheae; The 12-threeway; The 13-arm; The 14-arm; 15-gas head; The 16-annulus; The 17-straight tube.
Wherein, Fig. 1 is a Figure of abstract.
Embodiment
Below in conjunction with accompanying drawing and specific embodiment to the detailed description of the invention.
The structure of bioreactor of the present invention mainly is made up of right cylinder optical-biological reaction system 8, breather, proofing unit.Optical-biological reaction system 8 is that transparent glass, synthetic glass or resin material made.Add substratum and seed through opening for feed 7.The breather that cycles through of nutrient solution is regulated in the reactor drum.Breather is through inlet pipe 1 input air and carbonic acid gas; Ventilation Rate is measured by gas meter 4; Regulate by air intake valve 2; And purify through gas millipore filtration 3 pairs of air inlets, drive nutrient solution through gas flow and flow, thereby regulate liquid flow rate through air intake valve 2 adjustments of gas flow rates in bioreactor 8 circulating inside.
Proofing unit comprises pH detector, dissolved oxygen detector and Temperature Detector, inserts from bioreactor instrument probe mouth 6, can realize on-line monitoring.
When little algae is cultivated finite concentration and need collect; Close air intake valve 2; Utilize the settling character of little algae self or in nutrient solution, add flocculation agent to make little algae be deposited to reactor bottom, through right cylinder bottom liquid outlet 10 nutrient solution is discharged again, collect microalgae cell at last.
The breather of bioreactor is mainly by the gas head, and tracheae and threeway are formed.Main tracheae 11 is connected to an outlet of threeway 12 among Fig. 2, in addition two outlets and segmental bronchus 13 companies.Segmental bronchus 13 links to each other with another threeway, and threeway two outlets in addition is connected with arm 14.Arm 14 is connected with gas head 15.The gas head quantity that can be connected according to the size adjustment of algae kind of cultivating and required air flow.Said gas head can adopt the gas head of using in the common fish jar, perhaps other gas distributor.
Gas head 15 is tied up on annulus 16 among Fig. 3, is evenly distributed on around the annulus.Annulus can be a circle or many circles as required.
Gas head 15 is tied up on straight tube 17 among Fig. 4, linear array.

Claims (4)

1. simple and easy cylindrical reaction vessel for microalgae culture; Comprise optical-biological reaction system (8), breather and proofing unit; The top cover of optical-biological reaction system (8) can move freely; The breather source of the gas stretches into the bottom of bioreactor (8) through inlet pipe, it is characterized in that: the breather tree-shaped diffuser that gas head (15), tracheae and threeway (12) are formed of serving as reasons.
2. reactor drum according to claim 1 is characterized in that: tree-shaped diffuser is obtained the arm of arbitrary number through threeway by a person in charge.
3. according to described any one reactor drum of claim 1~2, it is characterized in that: breather is fixed on the object of annulus, straight tube and/or random shape.
4. according to described any one reactor drum of claim 1~2, it is characterized in that: breather, filter membrane and/or filter core are installed in admission passage.
CN 200810240025 2008-12-17 2008-12-17 Simple and easy cylindrical reaction vessel for microalgae culture Active CN101760429B (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
TWI637173B (en) * 2017-04-28 2018-10-01 中原大學 Reaction device for tiny organism and monitoring device for tiny organism using the same

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* Cited by examiner, † Cited by third party
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CN102586074A (en) * 2011-01-17 2012-07-18 张一熙 Immersed LED (Light-emitting Diode) light energy algae-raising photovoltaic power station system with efficient output
CN102816699A (en) * 2011-06-09 2012-12-12 广西科学院 Novel method for culturing algae by capturing light efficiently
CN102296025B (en) * 2011-08-29 2013-05-15 暨南大学 Photobioreactor capable of realizing internal and external circulation of alga liquid
CN103087919B (en) * 2013-01-16 2014-04-16 西北师范大学 Method and device for collecting microalgae through continuous culture and in-situ self-flocculation

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN87210089U (en) * 1987-07-13 1988-06-22 余兆丰 Simple apparatus for culturing liquid strain in depth layer
CN1187535A (en) * 1997-12-31 1998-07-15 华南理工大学 Miniature algae producing system of pipe air lift magnetic treatment optical biological reactor and monitoring method thereof
CN2303849Y (en) * 1997-06-13 1999-01-13 徐明芳 Optical-radiation tower plate type photobiological reactor
CN200999243Y (en) * 2007-01-05 2008-01-02 天津商学院 Optical bioreactor
CN101294132A (en) * 2008-06-19 2008-10-29 蔡志武 Gas push type photobioreactor

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN87210089U (en) * 1987-07-13 1988-06-22 余兆丰 Simple apparatus for culturing liquid strain in depth layer
CN2303849Y (en) * 1997-06-13 1999-01-13 徐明芳 Optical-radiation tower plate type photobiological reactor
CN1187535A (en) * 1997-12-31 1998-07-15 华南理工大学 Miniature algae producing system of pipe air lift magnetic treatment optical biological reactor and monitoring method thereof
CN200999243Y (en) * 2007-01-05 2008-01-02 天津商学院 Optical bioreactor
CN101294132A (en) * 2008-06-19 2008-10-29 蔡志武 Gas push type photobioreactor

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
TWI637173B (en) * 2017-04-28 2018-10-01 中原大學 Reaction device for tiny organism and monitoring device for tiny organism using the same

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