CN111624313A - Research device and method for allelopathy of volatile organic compounds of plant root system - Google Patents
Research device and method for allelopathy of volatile organic compounds of plant root system Download PDFInfo
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- CN111624313A CN111624313A CN202010581559.3A CN202010581559A CN111624313A CN 111624313 A CN111624313 A CN 111624313A CN 202010581559 A CN202010581559 A CN 202010581559A CN 111624313 A CN111624313 A CN 111624313A
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- 241000196324 Embryophyta Species 0.000 title claims abstract description 83
- 239000012855 volatile organic compound Substances 0.000 title claims abstract description 76
- 230000036531 allelopathy Effects 0.000 title claims abstract description 25
- 238000000034 method Methods 0.000 title claims abstract description 10
- 239000005416 organic matter Substances 0.000 claims abstract description 28
- -1 polytetrafluoroethylene Polymers 0.000 claims abstract description 21
- 229920001343 polytetrafluoroethylene Polymers 0.000 claims abstract description 21
- 239000004810 polytetrafluoroethylene Substances 0.000 claims abstract description 21
- 239000002689 soil Substances 0.000 claims abstract description 20
- 238000007789 sealing Methods 0.000 claims abstract description 17
- 244000005700 microbiome Species 0.000 claims abstract description 16
- 238000003556 assay Methods 0.000 claims abstract description 4
- 230000000813 microbial effect Effects 0.000 claims abstract description 4
- 229910001220 stainless steel Inorganic materials 0.000 claims description 20
- 239000010935 stainless steel Substances 0.000 claims description 20
- 230000000723 chemosensory effect Effects 0.000 claims description 8
- 230000000694 effects Effects 0.000 claims description 7
- 239000000463 material Substances 0.000 claims description 5
- 230000000149 penetrating effect Effects 0.000 abstract description 2
- 238000002474 experimental method Methods 0.000 description 8
- 230000028327 secretion Effects 0.000 description 8
- 239000004809 Teflon Substances 0.000 description 5
- 229920006362 Teflon® Polymers 0.000 description 5
- 239000011347 resin Substances 0.000 description 5
- 229920005989 resin Polymers 0.000 description 5
- 230000009286 beneficial effect Effects 0.000 description 2
- 239000000284 extract Substances 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 238000007792 addition Methods 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- 238000007599 discharging Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/0004—Gaseous mixtures, e.g. polluted air
- G01N33/0009—General constructional details of gas analysers, e.g. portable test equipment
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/02—Devices for withdrawing samples
- G01N1/22—Devices for withdrawing samples in the gaseous state
- G01N1/24—Suction devices
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/0004—Gaseous mixtures, e.g. polluted air
- G01N33/0009—General constructional details of gas analysers, e.g. portable test equipment
- G01N33/0027—General constructional details of gas analysers, e.g. portable test equipment concerning the detector
- G01N33/0036—General constructional details of gas analysers, e.g. portable test equipment concerning the detector specially adapted to detect a particular component
- G01N33/0047—Organic compounds
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/0098—Plants or trees
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- Immunology (AREA)
- General Physics & Mathematics (AREA)
- General Health & Medical Sciences (AREA)
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Abstract
The invention relates to the field of research on allelopathy of volatile organic compounds of plant root systems, in particular to a device and a method for researching the allelopathy of the volatile organic compounds of the plant root systems. The method for researching the allelopathy of the volatile organic compounds of the plant root system comprises the following steps: s1: placing the root system of the plant in a volatile organic matter collector, penetrating the stem of the plant through a rubber cylinder, placing the leaf part of the plant above the organic matter collector, and clamping the rubber cylinder by using a sealing clamp to seal the rubber cylinder; s2: placing plants, soil and microorganisms in a sample tray in the reactor; s3: the injector pumps out the air in the reactor through the rubber hose, and the volatile organic compounds collected in the volatile organic compound collector enter the reactor through the polytetrafluoroethylene tube to contact with plants, soil and microorganisms; s4: the assay studies were performed on plants, soil and microbial removal.
Description
Technical Field
The invention relates to the field of research on allelopathy of volatile organic compounds of plant root systems, in particular to a device and a method for researching the allelopathy of the volatile organic compounds of the plant root systems.
Background
The utility model discloses a root secretion collecting and allelopathy observing device, which has the application number of CN201920494165.7, and comprises a donor plant planting part, a root secretion collecting part, a root secretion separating part and an allelopathy observing part; a perforated clapboard is arranged between the planting part of the donor plant and the root secretion collecting part, and a liquid discharging hole is arranged on the perforated clapboard; the root secretion separation part is provided with a resin column connecting pipe, and resin is filled in the resin column connecting pipe; the allelopathy observation part consists of an experiment part and a comparison part, the experiment part is provided with a resin column connecting pipe and a plant cultivation pot, and the lower end of the resin column connecting pipe is connected with the plant cultivation pot; the contrast part is provided with a connecting pipe and a plant cultivation pot, the top of the connecting pipe is connected with the root system secretion collecting part through a movable switch, and the lower end of the connecting pipe is connected with the plant cultivation pot. The utility model discloses a simple structure, convenient to use, with low costs, can collect plant root secretion, simple differentiation, the allelopathy contrast observation of root secretion. However, the patent can not be used for researching the allelopathy of the volatile organic compounds of the plant root system.
Disclosure of Invention
The invention provides a device and a method for researching the allelopathy of a volatile organic compound of a plant root system, which have the beneficial effect that the allelopathy of the volatile organic compound of the plant root system can be researched.
The invention relates to the field of research on the chemosensory effect of volatile organic compounds of plant roots, in particular to a device for researching the chemosensory effect of the volatile organic compounds of the plant roots, which comprises a volatile organic compound collector, a sealing clamp, a rubber cylinder, a polytetrafluoroethylene tube, a reactor and a sample disc.
The reactor is characterized in that the volatile organic matter collector is communicated with the reactor through a polytetrafluoroethylene tube, a rubber cylinder is arranged on the upper portion of the volatile organic matter collector and communicated with the volatile organic matter collector, a sealing clamp is arranged at the position of the rubber cylinder, a sample disc is arranged in the reactor, and an air exhaust device is arranged on the upper portion of the reactor.
The air extractor comprises a rubber hose, an injector and an air outlet, the air outlet is arranged at the upper part of the reactor, the air outlet is connected with the rubber hose, and the other end of the rubber hose is connected with the injector.
Plant roots volatile organic compounds ization effect research device still includes stainless steel support, the downside fixedly connected with stainless steel support of sample dish, the lower part fixed connection of stainless steel support is on the inside bottom surface of reactor.
The stainless steel bracket and the sample tray are both made of stainless steel.
Plant roots volatile organic compounds ization effect research device still includes side cap, sealing washer and lug, and the front side of volatile organic compounds collector and reactor is all opened, and the side cap is provided with two, and equal an organic whole is provided with the lug on two side caps, and the border position of two lugs all bonds and has the side cap, and two lugs are inserted respectively in the front side of volatile organic compounds collector and reactor and are opened the department to open the department with the front side of volatile organic compounds collector and reactor and seal.
The volume of the volatile organic compound collector and the reactor is one cubic meter.
The volatile organic matter collector, the reactor and the two side covers are all made of polytetrafluoroethylene.
A method for researching the allelopathy of the volatile organic compounds in the root system of a plant comprises the following steps:
s1: placing the root system of the plant in a volatile organic matter collector, penetrating the stem of the plant through a rubber cylinder, placing the leaf part of the plant above the organic matter collector, and clamping the rubber cylinder by using a sealing clamp to seal the rubber cylinder;
s2: placing plants, soil and microorganisms in a sample tray in the reactor;
s3: the injector pumps out the air in the reactor through the rubber hose, and the volatile organic compounds collected in the volatile organic compound collector enter the reactor through the polytetrafluoroethylene tube to contact with plants, soil and microorganisms;
s4: the assay studies were performed on plants, soil and microbial removal.
The research device for the allelopathy of the volatile organic compounds of the plant root system has the beneficial effects that:
the invention relates to a device and a method for researching the allelopathy of a plant root system volatile organic compound.
Drawings
The invention is described in further detail below with reference to the accompanying drawings and specific embodiments.
FIG. 1 is a schematic view of the overall structure of a plant root system volatile organic compound allelopathy research device of the present invention;
FIG. 2 is a schematic view of the overall structure of a plant root system volatile organic compound allelopathy research apparatus of the present invention;
fig. 3 is a schematic structural view of the side cover.
In the figure: a volatile organic collector 1; a sealing clip 2; a rubber cylinder 3; a polytetrafluoroethylene tube 4; a reactor 5; a rubber hose 6; an injector 7; an air outlet 8; a sample tray 9; a stainless steel bracket 10; a side cover 11; a seal ring 12; and a bump 13.
Detailed Description
In the description of the present invention, it is to be understood that the terms "center", "longitudinal", "lateral", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", and the like, indicate orientations or positional relationships based on those shown in the drawings, and are used only for convenience in describing the present invention and for simplicity in description, and do not indicate or imply that the referenced devices or elements must have a particular orientation, be constructed and operated in a particular orientation, and thus, are not to be construed as limiting the present invention. Furthermore, the terms "first", "second", etc. are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first," "second," etc. may explicitly or implicitly include one or more of that feature. In the description of the present invention, "a plurality" means two or more unless otherwise specified.
The first embodiment is as follows:
the following describes the embodiment with reference to fig. 1 to 3, and the present invention relates to the field of research on the allelopathy of the volatile organic compounds of plant root systems, and more specifically, to a research apparatus for the allelopathy of the volatile organic compounds of plant root systems, which includes a volatile organic compound collector 1, a sealing clip 2, a rubber cylinder 3, a polytetrafluoroethylene tube 4, a reactor 5, and a sample tray 9.
The reactor is characterized in that the volatile organic compound collector 1 is communicated with the reactor 5 through a polytetrafluoroethylene tube 4, a rubber tube 3 is arranged on the upper portion of the volatile organic compound collector 1, the rubber tube 3 is communicated with the volatile organic compound collector 1, a sealing clamp 2 is arranged at the position of the rubber tube 3, a sample disc 9 is arranged in the reactor 5, and an air exhaust device is arranged on the upper portion of the reactor 5. When the device is used, the root system of a plant is placed in the volatile organic matter collector 1, the stem of the plant penetrates through the rubber cylinder 3, the blade part of the plant is placed above the organic matter collector 1, and then the rubber cylinder 3 is clamped by the sealing clamp 2, so that the rubber cylinder 3 is sealed; the volatile organic matter collector 1 is used for collecting volatile organic matters generated by a plant root system; during research, the volatile organic matter collector 1 and the reactor 5 are not communicated with the outside; plants, soil and microorganisms are placed in the sample tray 9 in the reactor 5, when the air extractor extracts air, the volatile organic compounds collected in the volatile organic compound collector 1 enter the reactor 5 through the polytetrafluoroethylene tube 4 to contact with the plants, the soil and the microorganisms, and then the influence of the volatile organic compounds released by the root system of the plants on the physiological and ecological characteristics of the adjacent plants, soil and microorganisms can be researched. The reason why the polytetrafluoroethylene tube 4 is used is that the polytetrafluoroethylene tube 4 does not adsorb and release volatile organic compounds, thereby avoiding the influence on the experiment.
The second embodiment is as follows:
the present embodiment is described below with reference to fig. 1 to 3, wherein the air extraction device comprises a rubber hose 6, an injector 7 and an air outlet 8, the air outlet 8 is arranged at the upper part of the reactor 5, the rubber hose 6 is connected to the air outlet 8, and the other end of the rubber hose 6 is connected to the injector 7. The injector 7 can pump out the air in the reactor 5 through the rubber hose 6, so that a negative pressure is generated in the reactor 5, and the volatile organic compounds collected in the volatile organic compound collector 1 enter the reactor 5 through the polytetrafluoroethylene tube 4 to contact with plants, soil and microorganisms.
The third concrete implementation mode:
referring to fig. 1-3, the present embodiment will be described, wherein the device for researching the allelopathy of the volatile organic compounds in the root system of a plant further comprises a stainless steel bracket 10, the stainless steel bracket 10 is fixedly connected to the lower side of the sample tray 9, and the lower part of the stainless steel bracket 10 is fixedly connected to the inner bottom surface of the reactor 5. The stainless steel support 10 acts to support the sample plate 9 and to hold the sample plate 9 up.
The fourth concrete implementation mode:
in the following, referring to fig. 1 to 3, the present embodiment will be described, in which the stainless steel holder 10 and the sample plate 9 are made of stainless steel. The chemical structure of the stainless steel material is stable, and the experiment is not influenced too much.
The fifth concrete implementation mode:
the following describes the present embodiment with reference to fig. 1 to 3, the device for researching the allelopathy of the volatile organic compounds in the plant root system further includes a side cover 11, a sealing ring 12 and two bumps 13, the front sides of the volatile organic compound collector 1 and the reactor 5 are both open, the side covers 11 are provided with two, the bumps 13 are provided on the two side covers 11, the side covers 11 are bonded to the edge positions of the two bumps 13, the two bumps 13 are respectively inserted into the front side open positions of the volatile organic compound collector 1 and the reactor 5, and the front side open positions of the volatile organic compound collector 1 and the reactor 5 are closed. Two side covers 11 can be dismantled, are convenient for put into experimental article in volatile organic compounds collector 1 and reactor 5, and sealing washer 12 has played sealed effect for volatile organic compounds collector 1 and reactor 5 do not communicate with the external world.
The sixth specific implementation mode:
in the following, the present embodiment will be described with reference to fig. 1 to 3, wherein the volume of the voc collector 1 and the volume of the reactor 5 are each one cubic meter.
The seventh embodiment:
in the following, referring to fig. 1 to 3, the volatile organic compound collector 1, the reactor 5 and the two side covers 11 are made of teflon. The reason why the volatile organic matter collector 1, the reactor 5 and the two side covers 11 use the teflon material is that the teflon does not adsorb and release the volatile organic matter, thereby avoiding the influence on the experiment.
A method for researching the allelopathy of the volatile organic compounds in the root system of a plant comprises the following steps:
s1: the root system of the plant is placed in the volatile organic matter collector 1, the stem of the plant penetrates through the rubber cylinder 3, the blade part of the plant is placed above the organic matter collector 1, and then the rubber cylinder 3 is clamped by the sealing clamp 2, so that the rubber cylinder 3 is sealed;
s2: placing plants, soil and microorganisms in a sample tray 9 in the reactor 5;
s3: the injector 7 pumps out the air in the reactor 5 through the rubber hose 6, and the volatile organic compounds collected in the volatile organic compound collector 1 enter the reactor 5 through the polytetrafluoroethylene tube 4 to contact with plants, soil and microorganisms;
s4: the assay studies were performed on plants, soil and microbial removal.
The working principle of the invention is as follows: when the device is used, the root system of a plant is placed in the volatile organic matter collector 1, the stem of the plant penetrates through the rubber cylinder 3, the blade part of the plant is placed above the organic matter collector 1, and then the rubber cylinder 3 is clamped by the sealing clamp 2, so that the rubber cylinder 3 is sealed; the volatile organic matter collector 1 is used for collecting volatile organic matters generated by a plant root system; during research, the volatile organic matter collector 1 and the reactor 5 are not communicated with the outside; plants, soil and microorganisms are placed in the sample tray 9 in the reactor 5, when the air extractor extracts air, the volatile organic compounds collected in the volatile organic compound collector 1 enter the reactor 5 through the polytetrafluoroethylene tube 4 to contact with the plants, the soil and the microorganisms, and then the influence of the volatile organic compounds released by the root system of the plants on the physiological and ecological characteristics of the adjacent plants, soil and microorganisms can be researched. The reason why the polytetrafluoroethylene tube 4 is used is that the polytetrafluoroethylene tube 4 does not adsorb and release volatile organic compounds, thereby avoiding the influence on the experiment. The injector 7 can pump out the air in the reactor 5 through the rubber hose 6, so that a negative pressure is generated in the reactor 5, and the volatile organic compounds collected in the volatile organic compound collector 1 enter the reactor 5 through the polytetrafluoroethylene tube 4 to contact with plants, soil and microorganisms. The stainless steel support 10 acts to support the sample plate 9 and to hold the sample plate 9 up. The chemical structure of the stainless steel material is stable, and the experiment is not influenced too much. Two side covers 11 can be dismantled, are convenient for put into experimental article in volatile organic compounds collector 1 and reactor 5, and sealing washer 12 has played sealed effect for volatile organic compounds collector 1 and reactor 5 do not communicate with the external world. The reason why the volatile organic matter collector 1, the reactor 5 and the two side covers 11 use the teflon material is that the teflon does not adsorb and release the volatile organic matter, thereby avoiding the influence on the experiment.
It is to be understood that the above description is not intended to limit the present invention, and the present invention is not limited to the above examples, and that various changes, modifications, additions and substitutions which are within the spirit and scope of the present invention and which may be made by those skilled in the art are also within the scope of the present invention.
Claims (8)
1. The utility model provides a plant roots volatile organic compounds ization effect research device, includes volatile organic compounds collector (1), seals and presss from both sides (2), a rubber tube (3), polytetrafluoroethylene pipe (4), reactor (5) and sample dish (9), its characterized in that: the reactor is characterized in that the volatile organic matter collector (1) is communicated with the reactor (5) through a polytetrafluoroethylene tube (4), a rubber cylinder (3) is arranged on the upper portion of the volatile organic matter collector (1), the rubber cylinder (3) is communicated with the volatile organic matter collector (1), a sealing clamp (2) is arranged at the rubber cylinder (3), a sample disc (9) is arranged in the reactor (5), and an air extractor is arranged on the upper portion of the reactor (5).
2. The device for researching the chemosensory effect of the volatile organic compounds in the root system of the plant as claimed in claim 1, is characterized in that: the air extractor comprises a rubber hose (6), an injector (7) and an air outlet (8), the air outlet (8) is arranged at the upper part of the reactor (5), the air outlet (8) is connected with the rubber hose (6), and the other end of the rubber hose (6) is connected with the injector (7).
3. The device for researching the chemosensory effect of the volatile organic compounds in the root system of the plant as claimed in claim 2, is characterized in that: plant roots volatile organic compounds ization effect research device still includes stainless steel support (10), downside fixedly connected with stainless steel support (10) of sample dish (9), the lower part fixed connection of stainless steel support (10) is on the inside bottom surface of reactor (5).
4. The device for researching the chemosensory effect of the volatile organic compounds in the root system of the plant as claimed in claim 3, is characterized in that: the stainless steel bracket (10) and the sample plate (9) are both made of stainless steel materials.
5. The device for researching the chemosensory effect of the volatile organic compounds in the root system of the plant as claimed in claim 4, is characterized in that: plant roots volatile organic compounds ization effect research device still includes side cap (11), sealing washer (12) and lug (13), the front side of volatile organic compounds collector (1) and reactor (5) is all opened, side cap (11) are provided with two, the homogeneous body is provided with lug (13) on two side caps (11), the border position of two lug (13) all bonds has side cap (11), the front side open department of inserting volatile organic compounds collector (1) and reactor (5) respectively in two lug (13), and close the front side open department of volatile organic compounds collector (1) and reactor (5).
6. The device for researching the chemosensory effect of the volatile organic compounds in the root system of the plant as claimed in claim 5, is characterized in that: the volume of the volatile organic compound collector (1) and the volume of the reactor (5) are both one cubic meter.
7. The device for researching the chemosensory effect of the volatile organic compounds in the root system of the plant as claimed in claim 6, is characterized in that: the volatile organic matter collector (1), the reactor (5) and the two side covers (11) are all made of polytetrafluoroethylene.
8. The method for researching the allelopathy of the volatile organic compounds of the plant root system according to the claim 7 comprises the following steps:
s1: the root system of the plant is placed in the volatile organic matter collector (1), the stem of the plant penetrates through the rubber cylinder (3), the blade part of the plant is placed above the organic matter collector (1), and then the rubber cylinder (3) is clamped by the sealing clamp (2) to seal the rubber cylinder (3);
s2: placing plants, soil and microorganisms in a sample tray (9) in the reactor (5);
s3: the injector (7) pumps out the air in the reactor (5) through the rubber hose (6), and the volatile organic compounds collected in the volatile organic compound collector (1) enter the reactor (5) through the polytetrafluoroethylene tube (4) to contact with plants, soil and microorganisms;
s4: the assay studies were performed on plants, soil and microbial removal.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010581559.3A CN111624313A (en) | 2020-06-23 | 2020-06-23 | Research device and method for allelopathy of volatile organic compounds of plant root system |
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| CN202010581559.3A CN111624313A (en) | 2020-06-23 | 2020-06-23 | Research device and method for allelopathy of volatile organic compounds of plant root system |
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| CN111624313A true CN111624313A (en) | 2020-09-04 |
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| CN202010581559.3A Pending CN111624313A (en) | 2020-06-23 | 2020-06-23 | Research device and method for allelopathy of volatile organic compounds of plant root system |
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| CN114323788A (en) * | 2021-12-23 | 2022-04-12 | 华南师范大学 | Plant root exudate in-situ collection device and method for collecting plant root exudate by using same |
| CN114631446A (en) * | 2022-03-31 | 2022-06-17 | 甘肃农业大学 | Allelopathy research device combining volatilization and leaching modes |
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Application publication date: 20200904 |