CN206706123U - A kind of cell culture bags - Google Patents
A kind of cell culture bags Download PDFInfo
- Publication number
- CN206706123U CN206706123U CN201720456153.6U CN201720456153U CN206706123U CN 206706123 U CN206706123 U CN 206706123U CN 201720456153 U CN201720456153 U CN 201720456153U CN 206706123 U CN206706123 U CN 206706123U
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- bag body
- frame piece
- bag
- body frame
- cell culture
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Abstract
Cell culture container technical field is the utility model is related to, especially a kind of cell culture bags.It include hermetic bag body, feed tube, liquid flowing tube, be placed in hermetic bag body haversack wall side C-type clamp bar and be placed in hermetic bag body flour bag wall side column press strip, column press strip is divided into upper and lower two independent sub- bag bodies corresponding to being pressed on the haversack wall of hermetic bag body and flour bag wall simultaneously in C clamp bar and by hermetic bag body, and liquid flowing tube is equiped with the flour bag wall per height bag body, is equiped with feed tube on side pocket wall.The utility model is used cooperatively by C-type clamp bar and column press strip, and clamping can be carried out to hermetic bag body makes receiving space to be separated into two independent receiving spaces (i.e.：Sub- bag body), operating personnel can be injected according to the requirement of the actual scale of construction to cell culture or extract a certain amount of cell tissue out, so as to realize effective control to cell culture amount by feed tube or liquid flowing tube by whole bag body or one of them sub- bag body.
Description
Technical field
Cell culture container technical field is the utility model is related to, especially a kind of cell culture bags.
Background technology
Cell culture (also known as in vitro culture, cell clone) refers to take out tissue out of living animal body, in analogue body
Under the conditions of inside physiological environment etc. is specific, the incubation culture of cell is carried out, is allowed to survive and grows, cell culture works
It is widely used in the every field such as biology, medical science, new drug development, turns into one of most important basic science.
At present, the culture apparatus commonly used in cell culture technology mainly includes culture dish, blake bottle and culture bag；Wherein,
Fairly large cell culture is being carried out (as amplification immunocyte carries out adoptive immunotherapy, extensive amplifying cells packaging
Virus and extensive amplifying cells obtain products of cellular metabolism) during, because bottleneck be present and open in culture dish and blake bottle
The features such as mouth is larger, sealing is poor so that be easy to overflow in the case that internally nutrient solution is more, so as to cause cell contamination
Probability increase；Meanwhile it is statically placed in the mode cultivated in culture dish or blake bottle and easily produces cell aggregation, local cells
The problem of density is big, so as to be unfavorable for exchange of air, cause the decline of cell proliferating number amount.Based on above mentioned problem, market at present
On there are cell culture bags made of a kind of high polymer material using gas-permeable, liquid-impermeable, bag body increase and extraneous can be utilized
Air exchange, and sealing property is relatively preferable；However, this culture bag is but present, cell culture amount is uncontrollable, is entering
Traveling liquid or the problems such as take liquid that mutual pollution occurs when operating.
Utility model content
For above-mentioned the shortcomings of the prior art, the purpose of this utility model is to provide a kind of cell culture bags.
To achieve these goals, the utility model adopts the following technical scheme that：
A kind of cell culture bags, it includes a hermetic bag body with a receiving space, is equiped with the hermetic bag body
Several feed tubes and liquid flowing tube for being communicated respectively with the receiving space of hermetic bag body；It also includes the back of the body for being placed in hermetic bag body
The C-type clamp bar of bag wall side and a column press strip for being placed in the flour bag wall side of hermetic bag body and being used with the pairing of C-type clamp bar, the post
Shape press strip the haversack wall of hermetic bag body and flour bag wall are pressed on simultaneously corresponding to be divided into C clamp bar and by hermetic bag body on
Lower two independent sub- bag bodies, a liquid flowing tube, and each sub- bag are each equiped with the flour bag wall of the sub- bag body
At least one feed tube is equiped with the side pocket wall of body.
Preferably, the feed tube includes being placed in the receiving space of sub- bag body feed liquor needle tubing, through sub- bag body
Side pocket wall is distributed and connected with the feed liquor in the integrally formed feed liquor transition conduit of side pocket wall of sub- bag body and the outside for being placed in sub- bag body
Adapter, the feed liquor connecting tube, feed liquor needle tubing and feed liquor transition conduit are by plastic material injection molding, and the feed liquor connecting tube
Material hardness be more than feed liquor needle tubing material hardness and feed liquor transition conduit material hardness.
Preferably, the hermetic bag body includes a preceding bag body frame piece with two windows, one and preceding bag body frame chip architecture
The back pkt. body frame piece and screening that identical and overall profile edge and the overall profile edge of preceding bag body frame piece are connected as a single entity
On the window of bag body frame piece and on the window of the back pkt. body frame piece and bag face made of waterproof ventilated membrane, the column pressure before being placed on
Bar is folded the horizontal frame piece between two windows of preceding bag body frame piece with the horizontal frame piece of corresponding back pkt. body frame piece
Postpone corresponding to being pressed in C-type clamp bar, the liquid flowing tube is installed on the bag face in the window of preceding bag body frame piece, described
In the region that bag body frame piece is mutually connected with back pkt. body frame piece before feed tube is installed in.
Preferably, on the preceding bag body frame piece and in the horizontal frame piece corresponding with column press strip and on back pkt. body frame piece
And it is embedded with sheet metal in the horizontal frame piece corresponding with C-type clamp bar.
Preferably, one end of the column press strip is fixed in one end of C-type clamp bar, the other end shape of the C clamp bar
Cheng Youyi is used for the slotting ring of the other end contraposition sleeve for column press strip.
Preferably, the liquid flowing tube include being fixed on corresponding on bag face and axial cross section in I-shaped fixed seat,
Taking liquid transition conduit and take liquid connecting tube with take that the outer end of liquid transition conduit is connected as a single entity through fixed seat distribution, it is described to take
Liquid connecting tube, take liquid transition conduit and fixed seat by plastic material integrated injection molding, and it is described take liquid connecting tube material it is hard
Degree and the material hardness of fixed seat are more than the material hardness for taking liquid transition conduit.
As a result of such scheme, the utility model is used cooperatively by C-type clamp bar and column press strip, can be to sealing
Bag body, which carries out clamping, makes receiving space to be separated into two independent receiving spaces (i.e.：Sub- bag body), operating personnel can basis
Requirement to the actual scale of construction of cell culture, by feed tube or liquid flowing tube by whole bag body or one of them sub- bag body injection or
A certain amount of cell tissue is extracted out, so as to realize effective control to cell culture amount；Simultaneously as liquid flowing tube and feed tube point
In the different azimuth of hermetic bag body (relative distance Wei Yu not be can be understood as farther out and mouth of pipe direction is different), can avoid carrying out
Feed liquor operates or taken liquid to occur the problem of feed tube mutually pollutes with liquid flowing tube when operating；Its simple in construction, easy to operate, use
Flexibly, there is very strong practical value and market popularization value.
Brief description of the drawings
Fig. 1 is the cross section structure schematic diagram of the utility model embodiment；
Fig. 2 is the structural relation schematic diagram between the c-type card article of the utility model embodiment and column press strip.
Embodiment
Embodiment of the present utility model is described in detail below in conjunction with accompanying drawing, but the utility model can be by right
It is required that the multitude of different ways for limiting and covering is implemented.
As depicted in figs. 1 and 2, a kind of cell culture bags that the present embodiment provides, it, which includes one, has a receiving space
Hermetic bag body a, a C-type clamp bar b and one for being placed in hermetic bag body a haversack wall side are placed in hermetic bag body a flour bag wall side and and C
The column press strip c that type clamp bar b pairings use, is equiped with several receiving spaces with hermetic bag body a respectively on hermetic bag body a
The feed tube d and liquid flowing tube e communicated；Wherein, hermetic bag body a haversack wall and flour bag wall are pressed on pair by column press strip c simultaneously
Upper and lower two independent sub- bag body a1 are divided into the C clamp bar b answered and by hermetic bag body a, per height bag body a1 flour bag wall
On be equiped with a liquid flowing tube e, and be equiped with least one feed tube d on the side pocket wall per height bag body a1.It is in this way, logical
Being used cooperatively for C-type clamp bar b and column press strip c is crossed, can carry out clamping to hermetic bag body a enables receiving space to be separated into position
Two independent receiving space (i.e. two spatially relatively independent isolations in C-type clamp bar b and above and below column press strip c both sides
Sub- bag body a1), operating personnel can according to the requirement of the actual scale of construction to cell culture, with by one of feed tube d to
Different amounts of cell tissue is injected in whole hermetic bag body a or in one of them sub- bag body a1, so as to realize to cell culture
Effective control of amount, only need to will one of son by one of liquid flowing tube e when needing to extract quantitative cell
Cell tissue in bag body a1 is aspirated；Simultaneously as liquid flowing tube e and feed tube d are located at hermetic bag body a not respectively
(it can be understood as relative distance with orientation farther out and mouth of pipe direction be different), can avoid carrying out feed liquor operation or taking liquid to operate
When there is the problem of feed tube d and liquid flowing tube e mutually pollute.
To optimize whole feed tube d structure, improve the utensils such as feed tube d connection syringes or carry out independent close
Convenience during envelope operation, feed liquor needle tubing 11 that the feed tube d of the present embodiment includes being placed in sub- bag body a1 receiving space, pass through
It is through at sub- bag body a1 side pocket wall distribution and with the sub- bag body a1 integrally formed feed liquor transition conduit 12 of side pocket wall and being placed in son
The feed liquor connecting tube 13 in bag body a1 outside；Wherein, feed liquor connecting tube 13, feed liquor needle tubing 11 and feed liquor transition conduit 12 are by plastic cement
Material injection is molded, and the material hardness of feed liquor connecting tube 13 is more than the material hardness and feed liquor transition conduit 12 of feed liquor needle tubing 11
Material hardness.Thus, using the otherness of the material hardness between each pipeline section, feed liquor connecting tube 13 can be easy to such as injecting
The utensil such as device or sealing-plug carries out assembly connection, and the material hardness of feed liquor needle tubing 11 is relatively small, and it can be avoided to puncture sealing
Bag body a bag wall.
To optimize the structure of whole culture bag to greatest extent, the hermetic bag body a of the present embodiment, which includes one, has two windows
Mouthful preceding bag body frame piece 21, the one profile edge identical and overall with 21 structures of preceding bag body frame piece and preceding bag body frame piece 21 it is whole
Back pkt. body frame piece 22 that the profile edge of body is connected as a single entity and cover on the window of preceding bag body frame piece 21 and back pkt. body frame piece
The bag face 23 on 21 window and made of waterproof ventilated membrane, column press strip c by positioned at two windows of preceding bag body frame piece 21 it
Between horizontal frame piece be laid out with the horizontal frame piece of corresponding back pkt. body frame piece 22 after be pressed on corresponding in C-type clamp bar b, and
Liquid flowing tube e is then installed on the bag face 23 in the window of preceding bag body frame piece 21, before feed tube d is installed in bag body frame piece 21 with
In the region of the phase of back pkt. body frame piece 22 linking.Thus, using preceding bag body frame piece 21 and back pkt. body frame piece 22 as whole sealing
Bag body a skeleton, so that bag body has certain stereotyped structure, recycles the bag face 23 made of waterproof ventilated membrane to be combined into one
Individual complete and closing bag shaped structure, to ensure that culture bag has certain air change efficiency, is carried for the growing of cell
For condition；Simultaneously as column press strip c is pressed on after frame piece is laid out in C-type clamp bar b, can not only avoid pair
The problems such as bag face 23 causes such as abrasion, scraped, and be easy to be separated the accommodation space of culture bag, to form sub- bag
Body a1.
To further enhance after frame piece is stacked and presses, make between the sub- bag body a1 of adjacent two can fully every
From on preceding bag body frame piece 21 and in the horizontal frame piece corresponding with column press strip c and on back pkt. body frame piece 22 and and C-type clamp
Sheet metal f is embedded with horizontal frame piece corresponding bar b.Thus, it can be kept for two be stacked relatively using sheet metal f
Horizontal frame piece can be tightly bonded together in the presence of column press strip c, so as to realize the sky between antithetical phrase bag body a1
Between isolation effect.
To improve the effect that column press strip c and C-type clamp bar b is used in conjunction with each other, c-type is fixed in column press strip c one end
In clamp bar b one end, while it is used to supply the column press strip c other end to align sleeve formed with one in the C clamp bar b other end
Slotting ring g.Thus, when needing to carry out space separation to culture bag using column press strip c and C-type clamp bar b, it is only necessary to by column pressure
Bar c one end clamps after being extracted from slotting ring g to culture bag, is re-inserted into slotting ring g.
As a preferred scheme, the liquid flowing tube e of this city embodiment includes being fixed on corresponding bag face 23 and axially cut
Face takes liquid transition conduit 32 and with taking the outer of liquid transition conduit 32 in the fixed seat 31 of I-shaped, through what fixed seat 31 was distributed
Hold what is be connected as a single entity to take liquid connecting tube 33, take liquid connecting tube 33, take liquid transition conduit 32 and fixed seat 31 by plastic material one
Injection molding, and take liquid connecting tube 33 material hardness and fixed seat 31 material hardness be more than take the material of liquid transition conduit 32 hard
Degree.
Preferred embodiment of the present utility model is the foregoing is only, not thereby limits the scope of the claims of the present utility model,
Every equivalent structure made using the utility model specification and accompanying drawing content or equivalent flow conversion, or directly or indirectly fortune
Used in other related technical areas, similarly it is included in scope of patent protection of the present utility model.
Claims (6)
1. a kind of cell culture bags, it includes a hermetic bag body with a receiving space, if being equiped with the hermetic bag body
Dry the feed tube and liquid flowing tube that are communicated respectively with the receiving space of hermetic bag body；It is characterized in that：It is also placed in close including one
The C-type clamp bar of the haversack wall side of envelope body and a column for being placed in the flour bag wall side of hermetic bag body and being used with the pairing of C-type clamp bar
The haversack wall of hermetic bag body and flour bag wall are pressed in corresponding C clamp bar and will sealings by press strip, the column press strip simultaneously
Bag body is divided into upper and lower two independent sub- bag bodies, and a liquid flowing tube is equiped with the flour bag wall of each sub- bag body, and
At least one feed tube is equiped with the side pocket wall of each sub- bag body.
A kind of 2. cell culture bags as claimed in claim 1, it is characterised in that：The feed tube includes being placed in the appearance of sub- bag body
Receive feed liquor needle tubing in space, through sub- bag body the distribution of side pocket wall and with the integrally formed feed liquor mistake of side pocket wall of sub- bag body
Cross pipe and be placed in the feed liquor connecting tube in the outside of sub- bag body, the feed liquor connecting tube, feed liquor needle tubing and feed liquor transition conduit by
Plastic material injection molding, and the material hardness of the feed liquor connecting tube is more than the material hardness and feed liquor transition conduit of feed liquor needle tubing
Material hardness.
A kind of 3. cell culture bags as claimed in claim 1 or 2, it is characterised in that：The hermetic bag body, which includes one, has two
The preceding bag body frame piece of individual window, a profile edge identical and overall with preceding bag body frame chip architecture and the entirety of preceding bag body frame piece
The back pkt. body frame piece that is connected as a single entity of profile edge and cover on the window of preceding bag body frame piece and back pkt. body frame piece window
The upper and bag face made of waterproof ventilated membrane, the column press strip is by the horizontal frame between two windows of preceding bag body frame piece
Piece is pressed on after being laid out with the horizontal frame piece of corresponding back pkt. body frame piece in corresponding C-type clamp bar, the liquid flowing tube installing
In on the bag face in the window positioned at preceding bag body frame piece, bag body frame piece is mutually connected with back pkt. body frame piece before the feed tube is installed in
Region in.
A kind of 4. cell culture bags as claimed in claim 3, it is characterised in that：On the preceding bag body frame piece and with column press strip
Metal foil is embedded with corresponding horizontal frame piece and on back pkt. body frame piece and in the horizontal frame piece corresponding with C-type clamp bar
Piece.
A kind of 5. cell culture bags as claimed in claim 3, it is characterised in that：C-type is fixed in one end of the column press strip
In one end of clamp bar, the other end of the C clamp bar is used for the slotting ring of the other end contraposition sleeve for column press strip formed with one.
A kind of 6. cell culture bags as claimed in claim 3, it is characterised in that：The liquid flowing tube includes being fixed on corresponding bag
On face and axial cross section takes liquid transition conduit and with taking liquid transition conduit in the fixed seat of I-shaped, through fixed seat distribution
Outer end be connected as a single entity take liquid connecting tube, it is described to take liquid connecting tube, take liquid transition conduit and fixed seat by plastic material one
Injection molding, and the material hardness for taking liquid connecting tube and the material hardness of fixed seat are more than and take the material of liquid transition conduit hard
Degree.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720456153.6U CN206706123U (en) | 2017-04-27 | 2017-04-27 | A kind of cell culture bags |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720456153.6U CN206706123U (en) | 2017-04-27 | 2017-04-27 | A kind of cell culture bags |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN206706123U true CN206706123U (en) | 2017-12-05 |
Family
ID=60457545
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201720456153.6U Active CN206706123U (en) | 2017-04-27 | 2017-04-27 | A kind of cell culture bags |
Country Status (1)
| Country | Link |
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| CN (1) | CN206706123U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2021114088A1 (en) * | 2019-12-10 | 2021-06-17 | 苏州生动细胞生物科技有限公司 | Variable-volume cell culture bag |
-
2017
- 2017-04-27 CN CN201720456153.6U patent/CN206706123U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2021114088A1 (en) * | 2019-12-10 | 2021-06-17 | 苏州生动细胞生物科技有限公司 | Variable-volume cell culture bag |
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| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20220119 Address after: 518000 west side of floor 7, building a, No. 2, Luozu Industrial Avenue, Luozu community, Shiyan street, Bao'an District, Shenzhen, Guangdong Province Patentee after: Shenzhen Kekang Clinical Medicine Research Co.,Ltd. Address before: 518000 Room 201, building A, No. 1, Qian Wan Road, Qianhai Shenzhen Hong Kong cooperation zone, Shenzhen, Guangdong (Shenzhen Qianhai business secretary Co., Ltd.) Patentee before: SHENZHEN QIANHAI PRECISION BIO-TECH CO.,LTD. |