CN105018337A - Sample taking and material supplementing combined multifunctional shake flask fermentation equipment - Google Patents
Sample taking and material supplementing combined multifunctional shake flask fermentation equipment Download PDFInfo
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- CN105018337A CN105018337A CN201410177108.8A CN201410177108A CN105018337A CN 105018337 A CN105018337 A CN 105018337A CN 201410177108 A CN201410177108 A CN 201410177108A CN 105018337 A CN105018337 A CN 105018337A
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Abstract
The invention belongs to the field of biological medicine, and relates to sample taking and material supplementing combined multifunctional shake flask fermentation equipment. The equipment is composed of a flask body (1), a flask plug (2), a glass nozzle (3), a slilicone tube (4), a glass bead (5), a clamp (6), a slot (7), a three-opening material supplementing device (8) and a micropore filter (9). The glass nozzle (3) is formed in the bottom of the flask body (1) and connected with an outlet through the slilicone tube (4). The slilicone tube (4) is clamped tightly through the clamp (6). The glass bead (5) is arranged in the slilicone tube (4) to be used for opening/closing control for sample taking. The clamp (6) is loosened firstly when the sample is taken, and then the liquid is made to flow out of the slilicone tube (4) by pressing the bead and holding the bead between the fingers. The slot (7) is formed in the middle of the flask body (1), and the three-opening material supplementing device (8) can be right inserted into the slot. A feeding opening in the top of the three-opening material supplementing device (8) is connected with the micropore filter (9), the micropore filter (9) is taken down under the aseptic environment when material supplementing is conducted, an aseptic injector is connected, and substrate can be supplemented and added to a shake flask. Whether material supplementing is conducted or not is decided by the analysis result, obtained after the sample is taken, of fermentation liquid.
Description
Technical field
The invention belongs to biomedicine field, relate to a kind of multi-functional shaking flask fermentation unit, especially sample the fermentation unit be combined with feed supplement.
Background technology
The product of fermentation industry is widely used in the fields such as medicine, food, light industry, agriculture and animal husbandry, and if most microbiotic is all tunning, it is also tunning that semisynthetic antibiotics produces parent nucleus used.Shaking flask, as a kind of conventional fermentation unit, is widely used by Biopharmaceutical Enterprises, scientific research institutions of universities and colleges.Microorganism culturing is a continuous print fermenting process, for understanding the metabolism and growth situation of somatic cells in time, needs the change of sampling analysis fermentation parameter, observes somatic cells growth conditions and the situation such as whether to be polluted.Tradition shaking flask, owing to not having special sampling unit, needs to be taken out by fermented liquid by aseptic technique to analyze.Whole sampling process is time-consuming, effort, and once misoperation easily causes shaking flask microbiological contamination, makes subsequent fermentation hard to carry on.Meanwhile, traditional shaking flask, owing to not having feed supplementing device, when cell utilizes nutraceutical matrix in a large number, can cause nutrient to be consumed, the under-nutrition of follow-up phase; In addition, the branch in the middle part of culturing process of the moisture in shaking flask forms water evaporation and falls, and the longer water evaporation quantity of incubation time is larger, causes amount of liquid in shaking flask on the low side.
Chinese Patent Application No. 201320542659.0 provides a kind of shaking flask device of high-efficient culture fast sampling, it is characterized in that: be provided with silica gel plug at the opening part of bottle, silica gel plug is inserted with polypropylene tube and ventpipe, polypropylene tube upper end is connected with silicone tube, silicone tube is provided with clip, be provided with micropore air filtering membrane in ventpipe, polypropylene tube lower end is connected with agitating vane.Its weak point is at the bottom of pipe to be deep into bottle from top, needs first to be abandoned by the fermented liquid stayed during previous sample in polypropylene tube next time, because pipe is longer, cause the loss that fermented liquid is larger before sampling operation.
Chinese Patent Application No. 201320104916.2 provides a kind of shaking flask being convenient to sample, it is characterized in that: the sidewall of shaking flask bottle middle and upper part arranges opening, one end of grass tube is arranged on the bottom of shaking flask bottle inside, outside surface in the middle part of grass tube and the opening of bottle are tightly connected, the other end of grass tube is connected with silicone tube, and silicone tube is provided with the first tongs and the second tongs.Its weak point is at the bottom of to be pipe to be deep into bottle from shaking flask middle and upper part sidewall, and stopple coupon line length easily causes fermented liquid greater loss.
Summary of the invention
The object of the invention is to solve existing problem, a kind of multi-functional shaking flask fermentation unit is provided, sample in microorganism shake flask fermentation process can carry out easily to solve, the loss of fermented liquid can be reduced again as much as possible simultaneously, simultaneously can also according to the multi-functional shaking flask fermentation unit of the timely supplemental medium of the result of sampling analysis.
The object of the present invention is achieved like this: a kind of multi-functional shaking flask fermentation unit, comprise bottle (1), bottle stopper (2), glass mouth (3), silicone tube (4), granulated glass sphere (5), clip (6), slot (7), three mouthfuls of feeders (8), millipore filter (9) forms, it is characterized in that: a glass mouth (3) is offered in described bottle (1) bottom, outlet connects with silicone tube (4), silicone tube (4) clip (6) steps up, one granulated glass sphere (5) on-off control for sampling is set in pipe, bottle (1) middle part arranges a slot (7), three mouthfuls of feeders (8) just in time can be inserted in this slot.
The present invention also has some technical characteristics like this: described silicone tube (4) exit end bore is carefully narrow, a granulated glass sphere (5) is placed in pipe, silicone tube (4) clip (6) steps up, clip (6) is first unclamped during use, then by making liquid be flowed out by silicone tube (4) by pinching granulated glass sphere (5), sampling operation is completed.
The present invention also has some technical characteristics like this: described slot (7) device is that an opening is established at body (1) middle part, opening is arranged a cylindrical glass device wide at the top and narrow at the bottom, inner filling rubber pad, adhesion one cylindrical metal thread bush on glass device; One end of described three mouthfuls of feeders (8) is three mouthfuls, pipeline opening is connected with silicone tube, be connected to millipore filter (9), there is screw thread at middle part, corresponding with the screw thread on slot, a sharp tip is arranged at bottom, by puncturing rubber pad, together with being tightened on thread bush, constitute a whole set of feed supplementing device.
The present invention also has like this some technical characteristics: described sampling unit and feed supplementing device exist certain dependency, after sampling operation completes, according to the result analyzed sample liquid, determines whether feed supplement, and what substratum supplementary.
This multi-functional shaking flask fermentation unit, the object of convenient sampling can be reached, bottom shaking flask, manufacture stopple coupon simultaneously, sampling line is very short, the loss of fermented liquid can be avoided better, these are for needs repeatedly sampling operation, arranging sampling line from shaking flask top and have better beneficial effect undoubtedly more originally.Simultaneously, by feed supplementing device and sampling line collaborative design, according to the result of sampling analysis, to microorganism extra-nutrition material, millipore filter on three mouthfuls of feed supplementing devices is in communication with the outside, increase the gaseous interchange inside and outside bottle, improve the dissolved oxygen level in fermented liquid, facilitate shake flask fermentation effect better.
Accompanying drawing explanation
Below in conjunction with accompanying drawing, specific embodiments of the present invention are further described in detail.
Embodiment
The sampling unit of multi-functional shaking flask fermentation unit shown in Fig. 1 is made up of bottle (1), bottle stopper (2), glass mouth (3), silicone tube (4), granulated glass sphere (5), clip (6), described bottle (1) side bottom is offered a glass mouth (3) and is communicated with in bottle, form 1 outlet parallel with at the bottom of bottle, this outlet silicone tube (4) connects, silicone tube (4) clip (6) clamping no leakage, this silicone tube exit end diameter is tapered narrow, places a granulated glass sphere (5) in pipe.During sampling, first unclamp clip (6), then by making liquid be flowed out by silicone tube (4) by pinching pearl.Sampling operation simple and fast, fermented liquid loss is minimum.
The feed supplementing device of multi-functional shaking flask fermentation unit shown in Fig. 1 is made up of bottle (1), bottle stopper (2), slot (7), feeder (8), millipore filter (9).Described slot (7), for establishing an opening in body (1) middle part, opening is vertically arranged a cylindrical glass device wide at the top and narrow at the bottom, inner filling rubber pad, and glass device is fixed a cylindrical metal thread bush, and there is screw thread its inside; Described three mouthfuls of feeders (8) are made for metallic substance, and one end is three metal pipe mouth, and can supplement dissimilar matrix respectively, each mouth of pipe is connected with silicone tube, is connected to millipore filter, is connected with external environment; There is screw thread on three mouthfuls of feeder (8) whole body, and match with the screw thread on slot, a sharp tip is arranged at bottom, by puncturing rubber pad, together with being tightened on thread bush, constitutes a whole set of feed supplementing device.According to the analysis carried out fermented liquid after sampling, when needs feed supplement, this shake flask fermentation device is taken out from shaking table, be put on Bechtop, take off the millipore filter (9) being connected to the silica gel mouth of pipe, syringe sterilized in connection, injects shake flask fermentation device by the matrix in syringe by feed supplementing device.After feed supplement, on the silica gel mouth of pipe connects, millipore filter (9) carries out fermentable again.Feed operation is simple and quick, and the pipeline of feed supplement is simultaneously connected with millipore filter (9), makes shaking flask inside and ft connection, effectively can improve the dissolved oxygen in fermented liquid.
Claims (4)
1. a sampling, the multi-functional shaking flask fermentation unit that feed supplement combines, comprise bottle (1), bottle stopper (2), glass mouth (3), silicone tube (4), granulated glass sphere (5), clip (6), slot (7), three hole feeders (8), millipore filter (9) forms, it is characterized in that: a glass mouth (3) is offered in described bottle (1) bottom, outlet connects with silicone tube (4), silicone tube (4) clip (6) steps up, one granulated glass sphere (5) on-off control for sampling is set in pipe, bottle (1) middle part arranges a slot (7), three mouthfuls of feeders (8) just in time can be inserted in this slot.
2. according to the multi-functional shaking flask fermentation unit that sampling according to claim 1, feed supplement combine, it is characterized in that: described silicone tube (4) exit end bore is carefully narrow, in pipe, granulated glass sphere (5) is for being placed in silicone tube (4), silicone tube (4) clip (6) steps up, clip (6) is first unclamped, then by making liquid be flowed out by silicone tube (4) by pinching pearl during use.
3. according to the multi-functional shaking flask fermentation unit that sampling according to claim 1, feed supplement combine, it is characterized in that: described slot (7) device is that an opening is established at body (1) middle part, opening is arranged a cylindrical glass device wide at the top and narrow at the bottom, inner filling rubber pad, adhesion one cylindrical metal thread bush on glass device; One end of described three mouthfuls of feeders (8) is three mouthfuls, pipeline opening is connected with silicone tube, be connected to millipore filter (9), there is screw thread at middle part, corresponding with the screw thread on slot, a sharp tip is arranged at bottom, by puncturing rubber pad, together with being tightened on thread bush, constitute a whole set of feed supplementing device.
4. according to the multi-functional shaking flask fermentation unit that sampling according to claim 1, feed supplement combine, it is characterized in that: the situation of nutritive substance contained by the fermented liquid flowed out from silicone tube (4) by analysis determine whether carry out feed operation by three mouthfuls of feeders (8).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410177108.8A CN105018337A (en) | 2014-04-25 | 2014-04-25 | Sample taking and material supplementing combined multifunctional shake flask fermentation equipment |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410177108.8A CN105018337A (en) | 2014-04-25 | 2014-04-25 | Sample taking and material supplementing combined multifunctional shake flask fermentation equipment |
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| Publication Number | Publication Date |
|---|---|
| CN105018337A true CN105018337A (en) | 2015-11-04 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410177108.8A Pending CN105018337A (en) | 2014-04-25 | 2014-04-25 | Sample taking and material supplementing combined multifunctional shake flask fermentation equipment |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106520539A (en) * | 2016-12-05 | 2017-03-22 | 菏泽学院 | Multi-function instrument used for microbe acid and gas producing experiment and sulfur-containing amino acid detection experiment |
| CN113667601A (en) * | 2021-07-28 | 2021-11-19 | 江苏谱新生物医药有限公司 | Device and method for continuous culture and sampling of cells |
-
2014
- 2014-04-25 CN CN201410177108.8A patent/CN105018337A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106520539A (en) * | 2016-12-05 | 2017-03-22 | 菏泽学院 | Multi-function instrument used for microbe acid and gas producing experiment and sulfur-containing amino acid detection experiment |
| CN113667601A (en) * | 2021-07-28 | 2021-11-19 | 江苏谱新生物医药有限公司 | Device and method for continuous culture and sampling of cells |
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| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20151104 |
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| WD01 | Invention patent application deemed withdrawn after publication |