CN210916096U - Liquid supplementing device for cell culture bag - Google Patents
Liquid supplementing device for cell culture bag Download PDFInfo
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- CN210916096U CN210916096U CN201921674769.6U CN201921674769U CN210916096U CN 210916096 U CN210916096 U CN 210916096U CN 201921674769 U CN201921674769 U CN 201921674769U CN 210916096 U CN210916096 U CN 210916096U
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- feed liquor
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Abstract
The utility model discloses a fluid infusion device for cell culture bag, including liquid feeding bag, culture medium bottle and cultivation bag, liquid feeding bag's top and bottom be equipped with feed liquor pipe, drain pipe respectively, liquid feeding bag passes through feed liquor pipe intercommunication with culture medium bottle, liquid feeding bag passes through the drain pipe intercommunication with the cultivation bag, is equipped with the intercommunication or the closed condition of open bayonet socket control feed liquor pipe, drain pipe on feed liquor pipe, the drain pipe respectively, liquid feeding bag's top is equipped with the business turn over gas pocket, is equipped with the filter core in the business turn over gas pocket. The device has simple structure and convenient operation.
Description
Technical Field
The utility model relates to a medical appliance, in particular to a liquid supplementing device for a cell culture bag.
Background
Cell culture techniques, also known as cell cloning techniques, are used to obtain the desired cell metabolites, mainly by in vitro propagation. The method is widely applied to the fields of medical science, biology and other industries, and is the most central and important part of biotechnology.
Generally, the cell culture bottle and the culture dish are adopted for small-scale cell culture, the cell culture bag made of transparent materials can be selected for the cells with large demand, a very important link in the cell culture technology is to add liquid to the cells, a syringe is generally utilized to be connected with a liquid inlet of the culture bag in the liquid supplementing process, so that the culture solution is added into the culture bag, the range of the syringe is limited, the liquid flow cannot be controlled, and meanwhile, the push rod of the syringe also causes certain waste.
SUMMERY OF THE UTILITY MODEL
The to-be-solved technical problem of the utility model is to provide a fluid infusion device for cell culture bag, the device simple structure, convenient operation.
In order to solve the above technical problem, the utility model discloses a following technical means:
the utility model provides a fluid infusion device for cell culture bag, includes liquid feeding bag, culture medium bottle and cultivates the bag, liquid feeding bag's top and bottom be equipped with feed liquor pipe, drain pipe respectively, liquid feeding bag passes through feed liquor pipe intercommunication with culture medium bottle, liquid feeding bag passes through the drain pipe intercommunication with the cultivation bag, is equipped with open bayonet control feed liquor pipe, the intercommunication or the closed condition of drain pipe on feed liquor pipe, the drain pipe respectively, liquid feeding bag's top is equipped with the business turn over gas pocket, is equipped with the filter core in the business turn over gas pocket.
The pipe cap design of feed liquor pipe is the same with the pipe cap of culture medium bottle, can directly screw up the feed liquor pipe cap with cultivateing bottleneck internal rotation formula, and open bayonet socket is opened to the feed liquor pipe during the feed liquor, and the open bayonet socket is closed to the drain pipe, makes the culture solution flow enter the liquid feeding bag, confirms the liquid feeding volume according to the needs of liquid feeding, also can directly be with leading-in liquid feeding bag of whole culture solution, place 4 ℃ refrigerator in for use. When the volume of the liquid is required, the opening bayonet on the liquid inlet pipe can be closed, the opening bayonet on the liquid outlet pipe is opened, the culture solution is directly led into the culture bag through the liquid outlet pipe, and the liquid outlet pipe mouth is in sterile connection with the liquid inlet of the culture bag through the outward-rotating pipe cap.
The invention has simple structure, can save transfer time and material and production cost in the process of transferring cells among culture bags, thereby reducing the probability of cross contamination and improving the efficiency.
The further preferred technical scheme is as follows:
the liquid adding bag is a transparent bag body, and scales are marked on the surface of the bag body.
By setting scales, the amount of the liquid adding is conveniently judged and controlled.
The central position of the top of the liquid adding bag is provided with a hanging hole.
Through setting up the hanging hole, make things convenient for this device's connection fixed.
Drawings
Fig. 1 is a schematic structural diagram of the present invention.
Description of reference numerals: 1: a liquid adding bag; 12: an open type bayonet; 13: an open type bayonet; 14: a liquid inlet pipe; 15: a liquid outlet pipe; 16: a hanging hole; 17: and (4) air inlet and outlet.
Detailed Description
The present invention will be further described with reference to the following examples.
Referring to fig. 1, the fluid infusion device for cell culture bags of the present invention comprises a fluid infusion bag 1, a culture medium bottle and a culture bag; the liquid feeding bag is characterized in that a liquid inlet pipe 14 and a liquid outlet pipe 15 are respectively arranged at the top and the bottom of the liquid feeding bag 1, the liquid feeding bag 1 is communicated with the culture medium bottle through the liquid inlet pipe 14, the liquid feeding bag 1 is communicated with the culture bag through the liquid outlet pipe 15, open type bayonets 12 and 13 are respectively arranged on the liquid inlet pipe 14 and the liquid outlet pipe 15 to control the communication or closing state of the liquid inlet pipe 14 and the liquid outlet pipe 15, an air inlet and outlet hole 17 is formed in the top end of the liquid feeding bag 1, and a filter element is arranged in the air inlet.
The design of the pipe cap of the liquid inlet pipe 14 is the same as that of the pipe cap of the culture medium bottle, the liquid inlet pipe 14 and the culture bottle mouth can be screwed tightly in an internal rotation mode, the open bayonet 12 is opened by the liquid inlet pipe 14 during liquid inlet, the open bayonet 13 is closed by the liquid outlet pipe 15, the culture liquid flows into the liquid adding bag 1, the liquid adding volume is determined according to the liquid adding requirement, and all the culture liquid can be directly guided into the liquid adding bag 1 and placed in a refrigerator at 4 ℃ for standby. When the volume of the liquid is required, the open bayonet 12 on the liquid inlet pipe 14 can be closed, the open bayonet 13 on the liquid outlet pipe 15 is opened, the culture solution is directly led into the culture bag through the liquid outlet pipe 15, and the 15 mouth of the liquid outlet pipe is in aseptic connection with the liquid inlet of the culture bag through an outward-rotating pipe cap.
The liquid adding bag 1 is a transparent bag body, and scales are marked on the surface of the bag body.
By setting scales, the amount of the liquid adding is conveniently judged and controlled.
The central position of the top of the liquid adding bag 1 is provided with a hanging hole 16.
The hanging hole 16 is arranged, so that the device is convenient to connect and fix.
The cell transfer device is simple in structure, and can save transfer time and material and production cost in the cell transfer process between culture bags, so that the probability of cross contamination is reduced, and the efficiency is improved.
The above description is only a preferred and practical embodiment of the present invention, and not intended to limit the scope of the present invention, and all structural equivalents made by using the contents of the specification and drawings are included in the scope of the present invention.
Claims (3)
1. The utility model provides a fluid replacement device for cell culture bag, includes liquid feeding bag, culture medium bottle and culture bag, its characterized in that: the top and the bottom of liquid feeding bag be equipped with feed liquor pipe, drain pipe respectively, liquid feeding bag passes through feed liquor pipe intercommunication with the culture medium bottle, liquid feeding bag passes through the drain pipe intercommunication with the culture bag, be equipped with the intercommunication or the closed condition of open bayonet socket control feed liquor pipe, drain pipe on feed liquor pipe, the drain pipe respectively, liquid feeding bag's top is equipped with the business turn over gas pocket, is equipped with the filter core in the business turn over gas pocket.
2. The fluid replacement device for a cell culture bag according to claim 1, wherein: the liquid adding bag is a transparent bag body, and scales are marked on the surface of the bag body.
3. The fluid replacement device for a cell culture bag according to claim 1, wherein: the central position of the top of the liquid adding bag is provided with a hanging hole.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201921674769.6U CN210916096U (en) | 2019-10-09 | 2019-10-09 | Liquid supplementing device for cell culture bag |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201921674769.6U CN210916096U (en) | 2019-10-09 | 2019-10-09 | Liquid supplementing device for cell culture bag |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN210916096U true CN210916096U (en) | 2020-07-03 |
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ID=71364309
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201921674769.6U Active CN210916096U (en) | 2019-10-09 | 2019-10-09 | Liquid supplementing device for cell culture bag |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN210916096U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117305078A (en) * | 2023-09-25 | 2023-12-29 | 成都赛恩吉诺生物科技有限公司 | Tissue dissociation method |
| CN117327563A (en) * | 2023-09-25 | 2024-01-02 | 成都赛恩吉诺生物科技有限公司 | Tissue dissociation method |
-
2019
- 2019-10-09 CN CN201921674769.6U patent/CN210916096U/en active Active
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117305078A (en) * | 2023-09-25 | 2023-12-29 | 成都赛恩吉诺生物科技有限公司 | Tissue dissociation method |
| CN117327563A (en) * | 2023-09-25 | 2024-01-02 | 成都赛恩吉诺生物科技有限公司 | Tissue dissociation method |
| CN117327563B (en) * | 2023-09-25 | 2024-08-23 | 成都赛恩吉诺生物科技有限公司 | Tissue dissociation method |
| CN117305078B (en) * | 2023-09-25 | 2024-08-27 | 成都赛恩吉诺生物科技有限公司 | Tissue dissociation method |
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