CN211713120U - Hybridoma cell continuous culture device - Google Patents
Hybridoma cell continuous culture device Download PDFInfo
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- CN211713120U CN211713120U CN201922353913.2U CN201922353913U CN211713120U CN 211713120 U CN211713120 U CN 211713120U CN 201922353913 U CN201922353913 U CN 201922353913U CN 211713120 U CN211713120 U CN 211713120U
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- box
- culture
- receiving plate
- hybridoma cells
- material receiving
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Abstract
The utility model provides a hybridoma cell continuous culture device belongs to the cell culture device field. The continuous culture apparatus for hybridoma cells includes a collection unit, a culture unit, and a supply unit. The collecting part comprises a sealing box and a collecting box, and the collecting box is arranged in the sealing box. The culture part comprises a cylinder body, a support rod and a cell culture bed, the support rod is arranged in the cylinder body, the cell culture bed is fixedly connected with the support rod, and a liquid inlet and a material inlet are formed in the side wall of the cylinder body; the cells mainly gather on the surface of the cell culture bed, then the liquid conveying pipe is connected with the liquid inlet, the culture solution supply bottle is pressurized through the inflator, and the culture solution supply bottle starts to supply culture solution; the culture solution flows into a sealed box at a proper speed and is collected through a collecting box, and then the culture is obtained; easy control and cost reduction, simple operation, convenient control and suitability for most laboratories.
Description
Technical Field
The utility model relates to a cell culture device field particularly, relates to a hybridoma cell continuous culture device.
Background
At present, the hybridoma strain obtained by adopting a cell fusion technology is a common biological method for producing monoclonal antibodies by culturing the hybridoma strain, and the ascites obtained by injecting the hybridoma into a mouse is a basic method for producing high-titer antibodies, so that the method is high in cost and needs to breed a batch of mice.
In the prior art, a microcapsule immobilized cell culture method is adopted to continuously obtain a cell culture solution, and then an antibody is extracted from the culture solution. However, the method has high requirements on sterile conditions, various steps and high technical content, and is not easy to master.
SUMMERY OF THE UTILITY MODEL
In order to make up for the above deficiency, the utility model provides a hybridoma cell continuous culture device, aim at improving the not easily controlled, complex operation's of cell culture problem.
The utility model discloses a realize like this:
the utility model provides a hybridoma cell continuous culture device, including collection portion, culture portion and supply portion.
The collecting part comprises a sealing box and a collecting box, and the collecting box is arranged in the sealing box. The cell culture part comprises a cylinder body, a support rod and a cell culture bed, wherein the support rod is arranged in the cylinder body, the cell culture bed is fixedly connected with the support rod, a liquid inlet and a material inlet are formed in the side wall of the cylinder body, the cylinder body is fixed at the upper end of the seal box, and the bottom of the cylinder body is communicated with the inside of the seal box. The supply part comprises a culture solution supply bottle and a liquid conveying pipe, the culture solution supply bottle is fixed on the upper surface of the seal box, a bottle cap is fixed at the upper end opening of the culture solution supply bottle, the lower end of the liquid conveying pipe penetrates through the bottle cap and extends to the bottom of the culture solution supply bottle, the upper end of the liquid conveying pipe is connected with the liquid inlet, and the culture solution supply bottle is connected with an inflator.
The utility model discloses an in an embodiment, the seal box lateral wall has been seted up and has been got the material mouth, the outside sealing connection who gets the material mouth has the chamber door, the fixed surface has the callus on the sole under the seal box, the callus on the sole lower surface is provided with anti-skidding line.
The utility model discloses an in one embodiment, the collection box includes that first material receiving plate and second connect the flitch, first material receiving plate slides and pegs graft in the seal box, the second material receiving plate slides and pegs graft in the seal box, first material receiving plate sets up directly over the second material receiving plate, first material receiving plate and second material receiving plate upper surface all are seted up flutedly.
The utility model discloses an in the embodiment, logical groove has been seted up to the seal box upper surface, the seal box upper surface is fixed with the thread bush, the barrel lower extreme with thread bush threaded connection, be provided with sealed the pad in the thread bush.
The utility model discloses an in an embodiment, upper end of the support bar sliding connection has the sliding sleeve, the sliding sleeve lateral wall is fixed with the connecting rod, the one end of connecting rod with barrel inner wall fixed connection, upper end of the support bar is fixed with first magnet, the barrel surface is connected with second magnet.
In an embodiment of the present invention, the upper port of the cylinder is connected to a sealing cover, a sliding rod is fixed on the upper surface of the sealing cover, and the second magnet is slidably sleeved on the outer surface of the sliding rod.
In an embodiment of the present invention, the elastic member is sleeved on the upper end of the supporting rod, the upper end of the elastic member is connected to the first magnet, and the lower end of the elastic member is connected to the sliding sleeve.
The utility model discloses an in an embodiment, the gas vent has been seted up to the barrel lateral wall, inlet, feed inlet and gas vent port department all are provided with the valve body.
In an embodiment of the present invention, the cell culture bed comprises a first bed body and a second bed body, the first bed body is disposed on the upper side of the second bed body, and the surface of the first bed body is provided with through holes.
In an embodiment of the present invention, the air inlet is disposed on the surface of the bottle cap, and the air outlet of the inflator is connected to the air inlet through a pipe.
The utility model has the advantages that: the utility model discloses a hybridoma cell continuous culture device that obtains through above-mentioned design, during the use, at first carry out the full device sterilization to the barrel input ozone through the inlet, then slowly add the cell suspension to the cell culture bed through the feed inlet, like this, the cell mainly gathers in cell culture bed surface, then connects transfer line and inlet, pressurizes through the inflater for the culture solution supply bottle, and the culture solution supply bottle begins to supply with the culture solution; the culture solution flows into a sealed box at a proper speed and is collected through a collecting box, and then the culture is obtained; the method can be used for culturing suspension cell lines including hybridoma cell strains in different scales, simplifies the operation of suspension cell culture methods in different scales, is easy to control, can reduce the cost, is simple to operate and convenient to control, and is suitable for most laboratories.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings that are required to be used in the embodiments will be briefly described below, it should be understood that the following drawings only illustrate some embodiments of the present invention, and therefore should not be considered as limiting the scope, and for those skilled in the art, other related drawings can be obtained according to the drawings without inventive efforts.
Fig. 1 is a schematic structural diagram provided in an embodiment of the present invention;
fig. 2 is a schematic diagram of an internal structure provided in an embodiment of the present invention;
fig. 3 is a schematic structural diagram of a point a in fig. 2 according to an embodiment of the present invention;
FIG. 4 is a schematic view of a cell culture bed according to an embodiment of the present invention.
In the figure: 100-a collecting section; 110-a sealed box; 111-a door; 113-a foot pad; 115-thread bush; 117-gasket; 119-through groove; 130-a collection box; 131-a first receiving plate; 133-a second receiving plate; 300-a culture part; 310-a cylinder body; 311-a liquid inlet; 313-a feed port; 315-a sealing cover; 317-a slide bar; 319-exhaust port; 330-support rods; 331-a sliding sleeve; 333-connecting rod; 335-a first magnet; 337-a second magnet; 339-an elastic member; 350-a cell culture bed; 351-the first bed body; 3511-through hole; 353-a second bed body; 500-a supply section; 510-culture solution supply bottle; 511-bottle cap; 530-an infusion tube; 550-inflator.
Detailed Description
To make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the drawings of the embodiments of the present invention are combined to clearly and completely describe the technical solutions of the embodiments of the present invention, and obviously, the described embodiments are some embodiments of the present invention, not all embodiments. Based on the utility model provides a. Embodiments, all other embodiments obtained by a person skilled in the art without any inventive step are within the scope of the present invention.
Thus, the following detailed description of the embodiments of the present invention, presented in the accompanying drawings, is not intended to limit the scope of the invention, as claimed, but is merely representative of selected embodiments of the invention. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.
It should be noted that: like reference numbers and letters refer to like items in the following figures, and thus, once an item is defined in one figure, it need not be further defined and explained in subsequent figures.
In the description of the present invention, it is to be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and to simplify the description, but do not indicate or imply that the device or element referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore should not be construed as limiting the present invention.
Furthermore, the terms "first", "second" and "first" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of the present invention, "a plurality" means two or more unless specifically limited otherwise.
In the present invention, unless otherwise expressly stated or limited, the terms "mounted," "connected," and "fixed" are to be construed broadly and may, for example, be fixedly connected, detachably connected, or integrally formed; either directly or indirectly through intervening media, either internally or in any other relationship. The specific meaning of the above terms in the present invention can be understood according to specific situations by those skilled in the art.
In the present disclosure, unless expressly stated or limited otherwise, the first feature "on" or "under" the second feature may comprise direct contact between the first and second features, or may comprise contact between the first and second features not directly. Also, the first feature being "on," "above" and "over" the second feature includes the first feature being directly on and obliquely above the second feature, or merely indicating that the first feature is at a higher level than the second feature. A first feature being "under," "below," and "beneath" a second feature includes the first feature being directly under and obliquely below the second feature, or simply meaning that the first feature is at a lesser elevation than the second feature.
Examples
Referring to fig. 1-4, the present invention provides a technical solution: a continuous culture apparatus for hybridoma comprises a collecting part 100, a culture part 300, and a supply part 500.
Wherein, the collecting part 100 is arranged at the lower side of the culturing part 300 for collecting the cells growing in the culturing part 300, and the supplying part 500 is arranged at the upper side of the collecting part 100 for providing nutrient substances required by the cell growth for the culturing part 300 for controlling the culturing and collecting of the cells.
Referring to fig. 2, the collecting portion 100 includes a sealing box 110 and a collecting box 130, the collecting box 130 is disposed in the sealing box 110, a material taking opening is formed in a side wall of the sealing box 110, a box door 111 is hermetically connected to an outer side of the material taking opening, a foot pad 113 is fixedly connected to a lower surface of the sealing box 110 through a thread, anti-slip threads are disposed on a lower surface of the foot pad 113, the box door 111 is disposed to facilitate taking and placing of the collecting box 130, facilitate isolation of an external environment, and reduce contamination of the sealing box 110 by infectious microbes; the collecting box 130 comprises a first material receiving plate 131 and a second material receiving plate 133, the first material receiving plate 131 is inserted in the sealing box 110 in a sliding mode, the second material receiving plate 133 is inserted in the sealing box 110 in a sliding mode, the first material receiving plate 131 is arranged right above the second material receiving plate 133, grooves are formed in the upper surfaces of the first material receiving plate 131 and the second material receiving plate 133, and the first material receiving plate 131 and the second material receiving plate 133 are arranged up and down so as to be convenient for receiving cells and collecting and processing the cells.
Referring to fig. 3-4, the culture part 300 includes a cylinder 310, a support rod 330 and a cell culture bed 350, the cylinder 310 is a glass tube for facilitating cleaning and observing the inside, the cell culture bed 350 is an elastic fiber structure made of polystyrene material for facilitating cell attachment, the support rod 330 is disposed in the cylinder 310, a sliding sleeve 331 is slidably connected to the upper end of the support rod 330, a connecting rod 333 is adhesively fixed to the side wall of the sliding sleeve 331, one end of the connecting rod 333 is fixedly connected to the inner wall of the cylinder 310 by adhesive, a first magnet 335 is fixed to the upper end of the support rod 330, a second magnet 337 is connected to the outer surface of the cylinder 310, a sealing cover 315 is connected to the upper port of the cylinder 310, a sliding rod 317 is fixed to the upper surface of the sealing cover 315, the second magnet 337 is slidably sleeved to the outer surface of the sliding rod 317, an elastic member 339 is sleeved to the upper end of the, the lower end of the elastic element 339 is connected with the sliding sleeve 331, and the second magnet 337 is held by hand to drive the first magnet 335 inside the cylinder 310 to move, thereby indirectly driving the supporting rod 330 to move, so as to shake off cells on the cell culture bed 350. Cell culture bed 350 and bracing piece 330 fixed connection, cell culture bed 350 includes first bed body 351 and second bed body 353, first bed body 351 sets up at second bed body 353 upside, through-hole 3511 has been seted up on first bed body 351 surface, inlet 311 and feed inlet 313 have been seted up to barrel 310 lateral wall, barrel 310 is fixed in seal box 110 upper end, barrel 310 bottom and the inside intercommunication of seal box 110, logical groove 119 has been seted up to seal box 110 upper surface, surface mounting has thread bush 115 on the seal box 110, barrel 310 lower extreme and thread bush 115 threaded connection, be provided with sealed pad 117 in the thread bush 115, through threaded connection, be convenient for the dismantlement of barrel 310 is washd, facilitate the use.
Referring to fig. 2, the supply part 500 includes a culture solution supply bottle 510 and a liquid transfer tube 530, the culture solution supply bottle 510 is fixed on the upper surface of the seal box 110, a bottle slot is fixed on the upper surface of the seal box 110, the bottom of the culture solution supply bottle 510 is clamped in the bottle slot for easy taking, replacement and fixation, a bottle cap 511 is fixed on the upper port of the culture solution supply bottle 510, the lower end of the liquid transfer tube 530 penetrates through the bottle cap 511 and extends to the bottom of the culture solution supply bottle 510, the upper end of the liquid transfer tube 530 is connected with the liquid inlet 311, the side wall of the cylinder 310 is provided with an air outlet 319, valve bodies are arranged at the ports of the liquid inlet 311, the liquid inlet 313 and the air outlet 319, the culture solution supply bottle 510 is connected with an air.
The working principle of the continuous culture device for the hybridoma cells is as follows: when the device is used, firstly, ozone is input into the cylinder body 310 through the liquid inlet 311 for full device sterilization, then, cell suspension is slowly added into the cell culture bed 350 through the liquid inlet 313, cells mainly gather on the surface of the cell culture bed 350, then, the liquid conveying pipe 530 and the liquid inlet 311 are connected, the culture solution supply bottle 510 is pressurized through the inflator 550, and the culture solution supply bottle 510 starts to supply culture solution; the culture solution flows into the sealed box 110 at a proper speed and is collected by the collection box 130, i.e. a culture is obtained; the method can be used for culturing suspension cell lines including hybridoma cell strains in different scales, simplifies the operation of suspension cell culture methods in different scales, is easy to control, can reduce the cost, is simple to operate and convenient to control, and is suitable for most laboratories.
It should be noted that the specific model specification of the valve body needs to be determined by type selection according to the actual specification of the device, and the specific type selection calculation method adopts the prior art in the field, so detailed description is omitted.
The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention, and various modifications and changes may be made by those skilled in the art. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (10)
1. A continuous culture device for hybridoma cells is characterized by comprising
A collecting part (100), the collecting part (100) comprising a sealed box (110) and a collecting box (130), the collecting box (130) being arranged in the sealed box (110);
the culture part (300) comprises a cylinder body (310), a supporting rod (330) and a cell culture bed (350), the supporting rod (330) is arranged in the cylinder body (310), the cell culture bed (350) is fixedly connected with the supporting rod (330), a liquid inlet (311) and a liquid inlet (313) are formed in the side wall of the cylinder body (310), the cylinder body (310) is fixed at the upper end of the seal box (110), and the bottom of the cylinder body (310) is communicated with the inside of the seal box (110);
the feeding part (500) comprises a culture solution feeding bottle (510) and a liquid conveying pipe (530), the culture solution feeding bottle (510) is fixed on the upper surface of the seal box (110), a bottle cap (511) is fixed on the upper port of the culture solution feeding bottle (510), the lower end of the liquid conveying pipe (530) penetrates through the bottle cap (511) and extends to the bottom of the culture solution feeding bottle (510), the upper end of the liquid conveying pipe (530) is connected with the liquid inlet (311), and the culture solution feeding bottle (510) is connected with an inflator (550).
2. The continuous culture device for the hybridoma cells according to claim 1, wherein a material taking opening is formed in the side wall of the seal box (110), a box door (111) is connected to the outer side of the material taking opening in a sealing mode, a foot pad (113) is fixed to the lower surface of the seal box (110), and anti-skid lines are arranged on the lower surface of the foot pad (113).
3. The continuous culture device for the hybridoma cells according to claim 1, wherein the collection box (130) comprises a first material receiving plate (131) and a second material receiving plate (133), the first material receiving plate (131) is slidably inserted into the sealing box (110), the second material receiving plate (133) is slidably inserted into the sealing box (110), the first material receiving plate (131) is arranged right above the second material receiving plate (133), and grooves are formed in the upper surfaces of the first material receiving plate (131) and the second material receiving plate (133).
4. The continuous culture device for the hybridoma cells according to claim 1, wherein the sealing box (110) is provided with a through groove (119) on the upper surface, a threaded sleeve (115) is fixed on the upper surface of the sealing box (110), the lower end of the cylinder (310) is in threaded connection with the threaded sleeve (115), and a sealing gasket (117) is arranged in the threaded sleeve (115).
5. The continuous culture device for the hybridoma cells according to claim 1, wherein a sliding sleeve (331) is slidably connected to the upper end of the support rod (330), a connecting rod (333) is fixed to the side wall of the sliding sleeve (331), one end of the connecting rod (333) is fixedly connected to the inner wall of the cylinder (310), a first magnet (335) is fixed to the upper end of the support rod (330), and a second magnet (337) is connected to the outer surface of the cylinder (310).
6. The continuous culture device for the hybridoma cells according to claim 5, wherein a sealing cover (315) is connected to the upper port of the cylinder (310), a sliding rod (317) is fixed on the upper surface of the sealing cover (315), and the second magnet (337) is sleeved on the outer surface of the sliding rod (317) in a sliding manner.
7. The continuous culture apparatus for hybridoma cells according to claim 6, wherein an elastic member (339) is sleeved on the upper end of the support rod (330), the upper end of the elastic member (339) is connected with the first magnet (335), and the lower end of the elastic member (339) is connected with the sliding sleeve (331).
8. The continuous culture device for hybridoma cells according to claim 1, wherein the side wall of the cylinder (310) is opened with an exhaust port (319), and the ports of the liquid inlet (311), the liquid inlet (313) and the exhaust port (319) are provided with valve bodies.
9. The continuous culture device for the hybridoma cells according to claim 1, wherein the cell culture bed (350) comprises a first bed body (351) and a second bed body (353), the first bed body (351) is arranged on the upper side of the second bed body (353), and a through hole (3511) is formed in the surface of the first bed body (351).
10. The continuous culture device for the hybridoma cells according to claim 1, wherein the surface of the bottle cap (511) is provided with an air inlet, and an air outlet of the air pump (550) is connected with the air inlet through a pipeline.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201922353913.2U CN211713120U (en) | 2019-12-24 | 2019-12-24 | Hybridoma cell continuous culture device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201922353913.2U CN211713120U (en) | 2019-12-24 | 2019-12-24 | Hybridoma cell continuous culture device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN211713120U true CN211713120U (en) | 2020-10-20 |
Family
ID=72818475
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201922353913.2U Expired - Fee Related CN211713120U (en) | 2019-12-24 | 2019-12-24 | Hybridoma cell continuous culture device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN211713120U (en) |
-
2019
- 2019-12-24 CN CN201922353913.2U patent/CN211713120U/en not_active Expired - Fee Related
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20201020 Termination date: 20211224 |