CN109055216A - High-throughput 3D cell, class loading and organoid dynamic cultivation system - Google Patents
High-throughput 3D cell, class loading and organoid dynamic cultivation system Download PDFInfo
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- CN109055216A CN109055216A CN201811105729.XA CN201811105729A CN109055216A CN 109055216 A CN109055216 A CN 109055216A CN 201811105729 A CN201811105729 A CN 201811105729A CN 109055216 A CN109055216 A CN 109055216A
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- 238000011068 loading method Methods 0.000 title claims abstract description 34
- 210000002220 organoid Anatomy 0.000 title claims abstract description 33
- 230000010412 perfusion Effects 0.000 claims abstract description 59
- 239000007788 liquid Substances 0.000 claims abstract description 35
- 239000002699 waste material Substances 0.000 claims abstract description 26
- 238000002156 mixing Methods 0.000 claims abstract description 24
- 238000012258 culturing Methods 0.000 claims abstract description 20
- 230000002572 peristaltic effect Effects 0.000 claims abstract description 14
- 238000007599 discharging Methods 0.000 claims description 7
- 238000000746 purification Methods 0.000 claims description 6
- 238000004140 cleaning Methods 0.000 claims description 3
- 238000000034 method Methods 0.000 abstract description 4
- 239000012620 biological material Substances 0.000 abstract description 3
- 230000008569 process Effects 0.000 abstract description 2
- 239000007789 gas Substances 0.000 description 24
- 238000012549 training Methods 0.000 description 4
- 238000010586 diagram Methods 0.000 description 3
- 230000009471 action Effects 0.000 description 2
- 238000004113 cell culture Methods 0.000 description 2
- 238000005138 cryopreservation Methods 0.000 description 2
- 239000012530 fluid Substances 0.000 description 2
- 230000004907 flux Effects 0.000 description 2
- 210000000056 organ Anatomy 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 238000012604 3D cell culture Methods 0.000 description 1
- 208000030453 Drug-Related Side Effects and Adverse reaction Diseases 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 206010070863 Toxicity to various agents Diseases 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000011049 filling Methods 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000003285 pharmacodynamic effect Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 230000008929 regeneration Effects 0.000 description 1
- 238000011069 regeneration method Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 238000002560 therapeutic procedure Methods 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/08—Bioreactors or fermenters specially adapted for specific uses for producing artificial tissue or for ex-vivo cultivation of tissue
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
- C12M25/14—Scaffolds; Matrices
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/10—Perfusion
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Abstract
The invention discloses a kind of high throughput 3D cell, class loading and organoid dynamic cultivation systems, including casing ontology, the culture solution blending tank, peristaltic pump, perfusion culturing room, waste liquid storeroom and the pure gas source that the casing ontology side is arranged in and is connected to the culture solution blending tank that are successively set in the casing ontology along culture solution conveying direction.System provided by the invention can be used for the high-throughput dynamic cultivation of 3D cell, class loading, organoid, it is delivered in the high-throughput perfusion culture plate of perfusion cultivation region by the culture solution that peristaltic pump will be enriched in gas, the 3D dynamic cultivation of dimensional culture cell, class loading, organoid can be automatically completed.High-throughput 3D cell, class loading and organoid dynamic cultivation system whole process of the invention is safe and reliable, efficiently convenient, can meet the needs of different field researcher's dimensional culture, meet the developing direction of biomaterial He cell biology, there is good application value.
Description
Technical field
The present invention relates to cell biology, organizational project and biomaterial and field of biotechnology, in particular to a kind of high
Flux 3D cell, class loading and organoid dynamic cultivation system.
Background technique
Three-dimensional cell cultivation technology is relative to traditional two dimension culture there is many advantages, and researcher is from metabolism group
, protein expression all confirm this conclusion in nucleic acid level, and class loading, organoid belong to 3D cell culture, in length
Tissue, some key characteristics of organ are formed in phase incubation.In Developmental Biology, nosopathology, cell biology, regeneration
Mechanism, precisely medical treatment and multiple application field application potentials such as drug toxicity and pharmacodynamic test it is huge, especially in drug sieve
The accurate therapy field of choosing and tumor patient.
It is the problem that annoying researcher, cell 3D that how high throughput, which obtains and cultivates 3D cell, class loading and organoid,
The perfusion culture model of culture is to be conducive to three-dimensional cell culture material training method, but current perfusion culture using more at present
Flux is lower, it is difficult to meet requirement.Therefore, it is necessary to develop a kind of high passes for 3D cell, class loading, organoid
Measure 3D perfusion dynamic cultivation system.
Summary of the invention
In view of the above-mentioned deficiencies in the prior art, the technical problem to be solved by the present invention is that providing a kind of high throughput 3D
Cell, class loading and organoid dynamic cultivation system.
In order to solve the above technical problems, the technical solution adopted by the present invention is that: a kind of high throughput 3D cell, class loading and class
Organ dynamic cultivation system is successively set on the culture in the casing ontology including casing ontology, along culture solution conveying direction
Liquid blending tank, peristaltic pump, perfusion culturing room, waste liquid storeroom and setting the casing ontology side and with the culture solution
The pure gas source of blending tank connection.
Preferably, the pure gas source includes that gas cylinder and the gas mixing purification communicated therewith by air inlet pipeline fill
It sets.
Preferably, the culture solution blending tank is arranged in the left front portion of the casing ontology, and one end passes through outlet pipe
It is connected to the gas cleaning plant, the other end is connected to the perfusion culturing room by culture solution feeding pipe.
Preferably, the peristaltic pump is arranged on the culture solution feeding pipe, and it is left to be arranged in the casing ontology
Culture solution in the culture solution blending tank is pumped into the perfusion culturing room by rear portion.
Preferably, the perfusion culturing room is arranged in the middle part of the casing ontology, and discharge end passes through waste liquid discharge nozzle
Road is connected to the waste liquid storeroom.
Preferably, the lower part of the perfusion culturing room is provided with the first temperature control module, and upper part is provided with multiple cultures
Plate groove is equipped with high-throughput perfusion culture plate in the culture plate groove.
Preferably, the waste liquid storeroom is arranged in the casing ontology right part, and its underpart is provided with the second temperature control mould
Block.
Preferably, the two sides of the high-throughput perfusion culture plate are separately provided for and the culture solution feeding pipe
With the first female Luer and the second female Luer of waste liquid discharging piping connection.
Preferably, the high-throughput perfusion culture plate include the high-throughput perfusion culture solution that sets gradually from the bottom to top into
Liquid miniflow guidance tape, high-throughput perfusion culture solution go out liquid miniflow guidance tape, high-throughput perfusion culture intermediate plate and high-throughput perfusion culture
Plate lid.
Preferably, it is provided with flip on the casing ontology, transparent windows, the casing are provided in the flip
Touch screen and power knob are additionally provided on ontology.
The beneficial effects of the present invention are: system provided by the invention can be used for the high throughput of 3D cell, class loading, organoid
Dynamic cultivation is delivered in the high-throughput perfusion culture plate of perfusion cultivation region by the culture solution that peristaltic pump will be enriched in gas, can
Automatically complete the 3D dynamic cultivation of dimensional culture cell, class loading, organoid；It is avoided that in traditional culture systems because three-dimensional is trained
The excessive cell for causing three-dimensional rack center of scale of bracket and the cell on bracket lip edge are supported since the uneven factor of mass transfer causes
The unscientific problem of research model, high-throughput 3D cell of the invention, class loading and organoid dynamic cultivation system whole process
Securely and reliably, efficiently convenient, can meet the needs of different field researcher's dimensional culture, meet biomaterial and cell is raw
The developing direction of object has good application value.
Detailed description of the invention
Fig. 1 is the structural schematic diagram of high-throughput 3D cell of the invention, class loading and organoid dynamic cultivation system；
Fig. 2 is the schematic top plan view of high-throughput 3D cell of the invention, class loading and organoid dynamic cultivation system；
Fig. 3 is the structure of the casing ontology of high-throughput 3D cell of the invention, class loading and organoid dynamic cultivation system
Schematic diagram；
Fig. 4 is the high-throughput perfusion culture of high-throughput 3D cell of the invention, class loading and organoid dynamic cultivation system
The decomposition diagram of plate.
Description of symbols:
01 flip；02 transparent windows；03 air inlet pipeline；04 gas mixing purification device；05 gas cylinder；06 peristaltic pump；07
High-throughput perfusion culture plate；08 culture solution blending tank；09 touch screen；10 power knobs；11 culture solution feeding pipes；12 flip handles
Hand；13 first temperature control modules；14 waste liquid storerooms；15 waste liquids discharging pipeline；16 second temperature control modules；17 high-throughput perfusion cultures
Plate lid；18 high-throughput perfusion culture intermediate plates；19 high-throughput perfusion culture solutions go out liquid miniflow guidance tape；20 high-throughput perfusion culture solutions
Feed liquor miniflow guidance tape；30 casing ontologies；31-perfusion culturing room；32 outlet pipes.
Specific embodiment
The present invention will be further described in detail below with reference to the embodiments, to enable those skilled in the art referring to specification
Text can be implemented accordingly.
It should be appreciated that such as " having ", "comprising" and " comprising " term used herein are not precluded one or more
The presence or addition of a other elements or combinations thereof.
As shown in Figs 1-4, a kind of high throughput 3D cell, class loading and organoid dynamic cultivation system of the present embodiment, packet
Include casing ontology 30, the culture solution blending tank 08 that is successively set on along culture solution conveying direction in casing ontology 30, peristaltic pump 06,
Perfusion culturing room 31, waste liquid storeroom 14 and 30 side of casing ontology is set and is connected to culture solution blending tank 08 pure
Gas source.
Wherein, it is provided with flip 01 on casing ontology 30, transparent windows 02 are provided in flip 01, are also set in flip 01
It is equipped with flip handle 12, touch screen 09 and power knob 10 are additionally provided on casing ontology 30.
Pure gas source includes gas cylinder 05 and the gas mixing purification device 04 that is communicated therewith by air inlet pipeline 03.Gas is mixed
The control of mixing and its ratio that purification device 04 can realize that multiple gases carry out is closed, and clean processing can be carried out to gas.It is pure
Net gas source can guarantee cell, class loading, organoid 3D perfusion culture gaseous environment, gas group can also be controlled by pure gas source
Point.
Subregion forms culture solution mixed zone, 06th area of peristaltic pump, perfusion cultivation region and waste liquid storage area in casing ontology 30, with
Above-mentioned culture solution blending tank 08, peristaltic pump 06, perfusion culturing room 31 and waste liquid storeroom 14 is respectively set.Gas in gas cylinder 05
It is delivered to culture solution blending tank 08 after gas cleaning plant carries out clean processing, mixes with culture solution therein, is trained for perfusion
It supports room 31 and the culture solution for being rich in gas is provided；Under the action of peristaltic pump 06, the culture solution in culture solution blending tank 08 is pumped into filling
Culturing room 31 is flowed, is further delivered to waste liquid storeroom 14 later.
Wherein, the setting of culture solution blending tank 08 passes through outlet pipe 32 and gas in the left front portion of casing ontology 30, one end
Purification device connection, the other end are connected to perfusion culturing room 31 by culture solution feeding pipe 11.
Wherein, peristaltic pump 06 is arranged on culture solution feeding pipe 11, and is arranged in the left back portion of casing ontology 30, feeds
Mouth connection culture solution blending tank 08, discharge port connect the high-throughput perfusion culture plate 07 of perfusion culturing room 31.Peristaltic pump 06 can essence
The really perfusion rate of control culture solution by the culture liquid pump in culture solution blending tank 08 to perfusion culturing room 31, and then drives and arrives
Waste liquid chamber.
Wherein, the setting of perfusion culturing room 31 is connected at 30 middle part of casing ontology, discharge end by waste liquid discharging pipeline 15
To waste liquid storeroom 14.The lower part of perfusion culturing room 31 is provided with the first temperature control module 13, accurately control cell, class loading,
The cultivation temperature of organoid is to 37 DEG C；Upper part is provided with multiple culture plate grooves, is equipped with high pass in culture plate groove
Measure perfusion culture plate 07.
Wherein, waste liquid storeroom 14 is arranged in 30 right part of casing ontology, and its underpart is provided with the second temperature control module 16, can incite somebody to action
4 DEG C of cryo-conservations of waste liquid of cultivation region outflow.
Every piece of culture plate designs 64 channels using miniflow, and culture solution feed liquor fluid channel is respectively at culture with liquid fluid channel out
Female Luer female end is arranged in plate different height, different culture plate two sides, facilitates the connection of feeding pipe and the pipeline that discharges.
In one embodiment, the two sides of high-throughput perfusion culture plate 07 are separately provided for and culture solution feeding pipe
11 and waste liquid discharging pipeline 15 connect the first female Luer and the second female Luer (being not drawn into figure).It is high-throughput referring to Fig. 4
Perfusion culture plate 07 includes the high-throughput perfusion culture solution feed liquor miniflow guidance tape 20 set gradually from the bottom to top, high-throughput perfusion training
Nutrient solution goes out liquid miniflow guidance tape 19, high-throughput perfusion culture intermediate plate 18 and high-throughput perfusion culture plate lid 17.
In use, cell, class loading, the organoid of 3D culture are placed on the high-throughput perfusion training of high-throughput perfusion cultivation region
It supports in plate 07, and first temperature control module is controlled by touch screen 09, to carry out the control of cultivation temperature, meet cell training
Feeding temperature.Culture solution rich in gas provides gaseous environment and nutrition for the 3D cell, class loading, organoid culture of culture.
Culture solution after culture is delivered to waste liquid storeroom 14 through waste liquid discharging pipeline 15, and passes through the second temperature control of waste liquid storeroom 14
Module realizes cryo-conservation.
Although the embodiments of the present invention have been disclosed as above, but its is not only in the description and the implementation listed
With it can be fully applied to various fields suitable for the present invention, for those skilled in the art, can be easily
Realize other modification, therefore without departing from the general concept defined in the claims and the equivalent scope, the present invention is simultaneously unlimited
In specific details.
Claims (10)
1. a kind of high throughput 3D cell, class loading and organoid dynamic cultivation system, which is characterized in that including casing ontology, edge
Culture solution conveying direction is successively set on culture solution blending tank, peristaltic pump, perfusion culturing room, waste liquid storage in the casing ontology
Hiding room and the pure gas source that the casing ontology side is set and is connected to the culture solution blending tank.
2. high throughput 3D cell, class loading and organoid dynamic cultivation system according to claim 1, which is characterized in that
The pure gas source includes gas cylinder and the gas mixing purification device that is communicated therewith by air inlet pipeline.
3. high throughput 3D cell, class loading and organoid dynamic cultivation system according to claim 2, which is characterized in that
In the left front portion of the casing ontology, one end passes through outlet pipe and the gas cleaning plant for the culture solution blending tank setting
Connection, the other end are connected to the perfusion culturing room by culture solution feeding pipe.
4. high throughput 3D cell, class loading and organoid dynamic cultivation system according to claim 1, which is characterized in that
The peristaltic pump is arranged on the culture solution feeding pipe, and is arranged in the left back portion of the casing ontology, by the culture
Culture solution in liquid blending tank is pumped into the perfusion culturing room.
5. high throughput 3D cell, class loading and organoid dynamic cultivation system according to claim 4, which is characterized in that
The perfusion culturing room is arranged in the middle part of the casing ontology, and discharge end is connected to the waste liquid by waste liquid discharging pipeline and stores
Hide room.
6. high throughput 3D cell, class loading and organoid dynamic cultivation system according to claim 5, which is characterized in that
The lower part of the perfusion culturing room is provided with the first temperature control module, and upper part is provided with multiple culture plate grooves, the culture plate
High-throughput perfusion culture plate is equipped in groove.
7. high throughput 3D cell, class loading and organoid dynamic cultivation system according to claim 1, which is characterized in that
The waste liquid storeroom is arranged in the casing ontology right part, and its underpart is provided with the second temperature control module.
8. high throughput 3D cell, class loading and organoid dynamic cultivation system according to claim 6, which is characterized in that
The two sides of the high throughput perfusion culture plate are separately provided for connecting with the culture solution feeding pipe and waste liquid discharging pipeline
The first female Luer and the second female Luer connect.
9. high throughput 3D cell, class loading and organoid dynamic cultivation system according to claim 6, which is characterized in that
The high throughput perfusion culture plate includes the high-throughput perfusion culture solution feed liquor miniflow guidance tape set gradually from the bottom to top, high throughput
Perfusion culture solution goes out liquid miniflow guidance tape, high-throughput perfusion culture intermediate plate and high-throughput perfusion culture plate lid.
10. high throughput 3D cell, class loading and organoid dynamic cultivation system according to claim 1, which is characterized in that
It is provided with flip on the casing ontology, transparent windows are provided in the flip, touching is additionally provided on the casing ontology
Control screen and power knob.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811105729.XA CN109055216B (en) | 2018-09-21 | 2018-09-21 | High-throughput 3D cell, tissue-like and organ-like dynamic culture system |
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|---|---|---|---|
| CN201811105729.XA CN109055216B (en) | 2018-09-21 | 2018-09-21 | High-throughput 3D cell, tissue-like and organ-like dynamic culture system |
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| CN109055216A true CN109055216A (en) | 2018-12-21 |
| CN109055216B CN109055216B (en) | 2024-03-22 |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110144295A (en) * | 2019-05-31 | 2019-08-20 | 中国科学院苏州生物医学工程技术研究所 | Dynamic 3 D cell perfused culture system |
| CN110684642A (en) * | 2019-10-29 | 2020-01-14 | 康珞生物科技（武汉）有限公司 | Three-dimensional perfusion type cell culture instrument |
| CN110684658A (en) * | 2019-10-29 | 2020-01-14 | 康珞生物科技（武汉）有限公司 | Culture solution container storage device in three-dimensional perfusion type cell culture instrument |
| CN110684659A (en) * | 2019-10-29 | 2020-01-14 | 康珞生物科技（武汉）有限公司 | Culture bin ventilation circulation system in three-dimensional perfusion type cell culture instrument |
| CN110713924A (en) * | 2019-10-29 | 2020-01-21 | 康珞生物科技（武汉）有限公司 | Culture bin structure in three-dimensional cell culture instrument |
| CN110734858A (en) * | 2019-10-17 | 2020-01-31 | 康珞生物科技(常州)有限公司 | multi-mode three-dimensional perfusion type cell culture instrument |
| CN112300933A (en) * | 2020-10-30 | 2021-02-02 | 广州迈普再生医学科技股份有限公司 | Organoid molding apparatus and method |
| CN112940935A (en) * | 2021-01-29 | 2021-06-11 | 上海睿钰生物科技有限公司 | In-vitro life-sustaining perfusion culture system and control method thereof |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20080054500A (en) * | 2006-12-13 | 2008-06-18 | 주식회사 바이오트론 | Device for cell manipulation and cultivation in a closed environment |
| CN104611225A (en) * | 2015-02-16 | 2015-05-13 | 昆明市第一人民医院 | Bioreactor for constructing tissue engineering liver by using in-vitro circulatory perfusion |
| CN204779609U (en) * | 2015-02-16 | 2015-11-18 | 昆明市第一人民医院 | Extracorporal circulatory system perfusion founds bioreactor of organizational project liver |
| CN105586249A (en) * | 2016-03-07 | 2016-05-18 | 浙江大学 | Circulating perfusion biological reactor device capable of achieving circulating perfusion of three-dimensional support |
| CN107723236A (en) * | 2017-10-31 | 2018-02-23 | 广州迈普再生医学科技有限公司 | Dynamic perfusion culture systems |
-
2018
- 2018-09-21 CN CN201811105729.XA patent/CN109055216B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20080054500A (en) * | 2006-12-13 | 2008-06-18 | 주식회사 바이오트론 | Device for cell manipulation and cultivation in a closed environment |
| CN104611225A (en) * | 2015-02-16 | 2015-05-13 | 昆明市第一人民医院 | Bioreactor for constructing tissue engineering liver by using in-vitro circulatory perfusion |
| CN204779609U (en) * | 2015-02-16 | 2015-11-18 | 昆明市第一人民医院 | Extracorporal circulatory system perfusion founds bioreactor of organizational project liver |
| CN105586249A (en) * | 2016-03-07 | 2016-05-18 | 浙江大学 | Circulating perfusion biological reactor device capable of achieving circulating perfusion of three-dimensional support |
| CN107723236A (en) * | 2017-10-31 | 2018-02-23 | 广州迈普再生医学科技有限公司 | Dynamic perfusion culture systems |
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110144295A (en) * | 2019-05-31 | 2019-08-20 | 中国科学院苏州生物医学工程技术研究所 | Dynamic 3 D cell perfused culture system |
| CN110734858A (en) * | 2019-10-17 | 2020-01-31 | 康珞生物科技(常州)有限公司 | multi-mode three-dimensional perfusion type cell culture instrument |
| CN110734858B (en) * | 2019-10-17 | 2023-04-21 | 康珞生物科技(常州)有限公司 | Multi-mode three-dimensional perfusion type cell culture instrument |
| CN110684642A (en) * | 2019-10-29 | 2020-01-14 | 康珞生物科技（武汉）有限公司 | Three-dimensional perfusion type cell culture instrument |
| CN110684658A (en) * | 2019-10-29 | 2020-01-14 | 康珞生物科技（武汉）有限公司 | Culture solution container storage device in three-dimensional perfusion type cell culture instrument |
| CN110684659A (en) * | 2019-10-29 | 2020-01-14 | 康珞生物科技（武汉）有限公司 | Culture bin ventilation circulation system in three-dimensional perfusion type cell culture instrument |
| CN110713924A (en) * | 2019-10-29 | 2020-01-21 | 康珞生物科技（武汉）有限公司 | Culture bin structure in three-dimensional cell culture instrument |
| CN112300933A (en) * | 2020-10-30 | 2021-02-02 | 广州迈普再生医学科技股份有限公司 | Organoid molding apparatus and method |
| CN112300933B (en) * | 2020-10-30 | 2023-10-03 | 广州迈普再生医学科技股份有限公司 | Organoid molding device and method |
| CN112940935A (en) * | 2021-01-29 | 2021-06-11 | 上海睿钰生物科技有限公司 | In-vitro life-sustaining perfusion culture system and control method thereof |
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| Publication number | Publication date |
|---|---|
| CN109055216B (en) | 2024-03-22 |
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