CN206902165U - A kind of high efficiency culture systems of mass cell production and culture - Google Patents
A kind of high efficiency culture systems of mass cell production and culture Download PDFInfo
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- CN206902165U CN206902165U CN201720648446.4U CN201720648446U CN206902165U CN 206902165 U CN206902165 U CN 206902165U CN 201720648446 U CN201720648446 U CN 201720648446U CN 206902165 U CN206902165 U CN 206902165U
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Abstract
A kind of high efficiency culture systems of mass cell production and culture are the utility model is related to, the culture systems include at least one cell culture bags and at least one cell culture medium storage bag；The cell culture bags are used for cell culture, the cell culture medium storage bag is used to store the cell culture medium prepared, for the cell culture infused cells culture medium of cell culture bags, different specifications be present in cell culture bags and cell culture medium storage bag, it is usually separate state between each bag of body, it is necessary to ensure to carry out heat seal connection under germ-free condition using sterile heat seal bulb-tubulating machine when carrying out cell culture medium infusion, disconnect.Cell contamination and the probability of operating personnel's infection are reduced, improves the security of operation, improves the operating efficiency of cell culture.
Description
Technical field
It the utility model is related to cell Industrialized processing technique field, more particularly to a kind of production of mass cell and culture
High efficiency culture systems.
Background technology
Cell culture is cell under artificial environment, using nutriment etc., it is bred propagation, and be used for various mesh
Incubation.Cell culture has been widely used in scientific research, bio-pharmaceuticals, regenerative medicine, clinical practice and genetic engineering
Etc. every field.
With the development of the society, science and technology flourishes, the mankind there has also been the higher phase to quality of life and life expectancy
Hope.It is everyone dream to possess a health, happiness, happy life cycle.But such as persistent ailments such as tumours, traditional controls
Side effect also allows many patient sufferings can't bear caused by treatment means.
Cell therapy is the disease treatment new technology risen in recent years, refers to utilize some cells with specific function
Characteristic, after obtaining using biological engineering method and being modified by amplification in vitro, culture, make these cells that there is enhancing immunologic cytotoxicity
The therapeutic efficiency such as ability, specific recognition pathogen and tumour cell, promotion organization neomorph and physical recovery, so as to reach
Treat the purpose of disease.
The technology with it is good the effect of, unique advantage such as low side effect and personalization, for controlling for some refractory diseases
Treat, there is provided new selection.In the stage very long at present and in the future, cell therapy all will take on important angle in clinical treatment
Color, 21st century will be epoch that cell therapy plays a significant role.
" cell injuring model " is a most key, important ring in cell therapy technology.It is the basis of cell therapy,
It is directly connected to the effect and security for the treatment of.And it is huge for the cell quantity of patient's feedback in cell therapy, and matter
Amount requires high, it is therefore desirable to hundred grade working tables under ten thousand grades of extremely strict laboratory environments are cultivated for a long time,
The special Specialization education personnel for having received training for a long time are needed in incubation to be operated.Because the cell cultivated is finally
Need to feed back human body, it is therefore necessary to ensure the security of cultivated cell, it is absolutely not contaminated.Therefore in cell cultivation process
Gnotobasis is maintained to account for the most of the time of whole process, spent human and material resources and financial resources are immeasurable, even if
In well-appointed laboratory, if operator's technical operation is lack of standardization, pollution is also resulted in, ultimately results in a series for the treatment of
The delay of scheme, the treatment to patient damage.In high volume, key of the culture cell of high request as cell therapy, only
Growing scientific research, bio-pharmaceuticals, regenerative medicine, clinic can not be met by only relying on the culture of the special messenger under laboratory environment
Using with the demand such as genetic engineering, and many shortcomings also be present.Therefore a kind of new cell production systems and its correlation are needed badly
Matching component.
Utility model content
The utility model is the high efficiency culture systems that a kind of mass cell is produced and cultivated, compared to existing cell
Culture technique, the cell culture bags that the utility model use can completely cut off pollution environment carry out the overall process of cell culture, can be with
The step of simplifying cell culture, the rigors of cell culture are reduced, save cost.Carried out using ventilative cell culture bags
Cell culture, it is only necessary to which it is gnotobasis to ensure the environment in cell culture bags, the experiment sterile without requiring culture environment
Room and workbench, reduce and pollution risk existing for manual disinfecting process is used in laboratory, can greatly ensure cell culture
Security, simplify cell culture step.Using cell culture bags of the present utility model, need to can only be carried out under clean environment
The culture of cell.Secondly, step needed for cell cultivation process of the present utility model is overly simplified, without conventional cell culture
Every technical requirements, long-time professional training need not be carried out for cell culture personnel, you can operated, cell can be reduced
The difficulty of culture, it is adapted to the high-volume culture of cell.
To achieve the above objectives, the utility model adopts the technical scheme that：
A kind of production of mass cell and the high efficiency culture systems of culture, including at least one cell culture bags and at least
One cell culture medium storage bag；
The cell culture bags are used for cell culture, and cell culture bags have different specifications, the cell culture
Connecting tube is interval with the lower end sealing margin of bag, the connecting tube includes the first connecting tube, the second connecting tube and the 3rd connects
Adapter, the first connecting tube are used to be transfused culture medium, and the second connecting tube is used for the output of cell, and the 3rd connecting tube is used for the defeated of cell
Enter；
The cell culture medium storage bag is used to store the cell culture medium prepared, is that the cell culture of cell culture bags is defeated
Cell culture medium is noted, cell culture medium storage bag has different specifications, the lower end sealing strip of the cell culture medium storage bag
Connecting tube is provided with edge, the connecting tube includes the 4th connecting tube, the 5th connecting tube, and the 4th connecting tube, the 5th connecting tube are used for
To cell culture bags infused cells culture medium；
First connecting tube, the second connecting tube, the 3rd connecting tube, the 4th connecting tube, the mouth of pipe of the 5th connecting tube use
Heat seal mode seals.
On the basis of such scheme, described cell culture bags comprise at least the first cell culture bags and the second cell is trained
Bag is supported, when the connecting tube of the first described cell culture bags is connected with the connecting tube of the second described cell culture bags, using connecing
Tube method connects；
When the connecting tube of described cell culture bags is connected with the connecting tube of described cell culture medium storage bag, using connecing
Tube method connects,
Described pipe connection method connection, carries out heat seal connection by sterile heat seal mode or earnestly disconnects, pass through high temperature moment
Operation ensures sterile contamination.
On the basis of such scheme, the second connecting tube of the first described cell culture bags is trained with the second described cell
When supporting the 3rd connecting tube connection of bag, connected using pipe connection method；
4th connecting tube of the first connecting tube of described cell culture bags and described cell culture medium storage bag or the
When five connecting tubes connect, connected using pipe connection method.
Described cell culture bags is ensure that the cell concentration of culture in bag is maintained in a valid interval, cell culture
Bag is divided into different specifications from volume, and the cell culture bags of different size form this complete cultivating system.According to reality
The cell quantity of demand, the specifications of cell culture bags can constantly on prolong.During cell culture, according to cell initial number, typically
Cultivated since small dimension cell culture bags, the nutrient solution volume to former specification cell culture bags is unable to maintain that suitable cell is dense
Degree, then the cell culture bags cell that archaeocyte culture bag is exported to connecting tube and big one-level specification by cell input connecting tube,
The transfer of cell and nutrient solution in different cell culture bags is completed by adapter heat seal；After the completion of transfer, pass through thermal cut-out, separation
Two cell culture bags；
To maintain the cell density in incubation, when cell density raises, it is necessary to add cell culture medium.Cell is trained
Support the culture medium infusion connecting tube of base storage bag to connect with the culture medium input connecting tube of cell culture bags, completed by heat seal thin
Born of the same parents' nutrient solution inputs cell culture bags, passes through thermal cut-out, Dissociated cell culture bag and cell culture medium storage bag after the completion of infusion
Connecting tube, and hot sealing.
On the basis of such scheme, the specification of the cell culture bags includes 50ml, 200ml, 2000ml, 5000ml,
The specification of cell culture medium storage bag includes 50ml, 100ml, 200ml, to ensure to maintain suitable cell in cell cultivation process
Concentration is cultivated, and designs different size cell culture bags and cell culture medium storage bag, in cell cultivation process, by thin
Cell culture medium is added in born of the same parents' culture medium storage bag or changes the cell culture bags of bigger specification to maintain suitable cell culture
Concentration, according to the demand of different cell seeding density, protected using the cell culture bags and cell culture medium of dimension size
Deposit bag.
On the basis of such scheme, the cell culture medium storage bag body is provided with scale, is conveniently adjusted infusion culture
Base unit weight.
On the basis of such scheme, the material of the cell culture bags and connecting tube is held to meet plastics Aqueous injection
The highly-breathable material of device standard.
On the basis of such scheme, the highly-breathable material can select polyethylene, TPX or EVA etc..
On the basis of such scheme, the cell culture medium storage bag is made up of the airtight material of high barrier, because
It is made of air-locked material, can effectively ensure that the safety asepsis environment of culture medium, disposably volume can be prepared.
The application process of the high efficiency culture systems of the mass cell production and culture, comprises the following steps：
1st, cell culture medium is prepared, cell culture medium is placed in cell culture medium storage bag, is disposably prepared multiple thin
Born of the same parents' culture medium storage bag, it is stand-by that cell culture medium storage bag is positioned over cold storage in 4 DEG C of environment；Cell culture will be housed during use
The cell culture medium storage bag of base is warming up to 37 DEG C；
2nd, the cell of required culture is injected in cell culture bags by the 3rd connecting tube, while uses pipe connection method by cell
The first connection that the 4th connecting tube or the 5th connecting tube of culture medium storage bag pass through sterile heat seal bulb-tubulating machine and cell culture bags
Pipe heat seal connects, and cell culture medium is injected into cell culture bags by extruding, after cell culture medium infused, use is sterile
Heat seal bulb-tubulating machine high temperature moment blocks the 4th connecting tube or the 5th connecting tube and the first connecting tube, and by the 4th connecting tube or the 5th
The mouth of pipe heat seal of connecting tube and the first connecting tube, makes cell culture bags be separated with cell culture medium storage bag；
3rd, in cell cultivation process, the measure of cell culture concentration is carried out by turbidimetry, when cell culture is not suitable
, it is necessary to add note cell culture medium when preferably cultivating concentration, cell culture medium storage bag is taken, using sterile heat seal bulb-tubulating machine by cell
The first connecting tube heat seal connection that the 4th connecting tube or the 5th connecting tube of culture medium storage bag connection are connected with cell culture bags,
It can effectively ensure that in an aseptic environment, the cell culture medium in cell culture medium storage bag extruded into cell culture bags；
4th, after cell culture medium infused, the 4th connecting tube or are blocked using sterile heat seal bulb-tubulating machine high temperature moment
Five connecting tubes and the first connecting tube, and by the 4th connecting tube or the mouth of pipe heat seal of the 5th connecting tube and the first connecting tube, make
Cell culture bags separate with cell culture medium storage bag；
5th, as incubation time increases in cell cultivation process, when cell culture bags, can not to ensure that cell is suitably cultivated dense
It is thin by what is cultivated in archaeocyte culture bag by sterile heat seal bulb-tubulating machine, it is necessary to change the cell culture bags of bigger specification when spending
Born of the same parents are transferred in the bigger cell culture bags of specification by the second connecting tube, continue to cultivate；
6th, after the completion of the cell transfer cultivated in archaeocyte culture bag, by sterile heat seal bulb-tubulating machine by archaeocyte culture bag
Separated with existing cell culture bags.
On the basis of such scheme, the cell culture medium includes cell production factor, serum free medium and basis
Culture medium.
On the basis of such scheme, the cell category of the cell culture includes suspension cell and attached cell, described
Suspension cell species includes immunocyte, and attached cell species includes mescenchymal stem cell and IPS and its induction differentiation stem cell；
The cell culture mode includes suspend culture and adhere-wall culture.
On the basis of such scheme, the cell culture concentration in the cell culture bags, it is determined using turbidimetry,
By using spectrophotometric determination cell culture fluid absorbance, cell concentration standard curve is contrasted to determine described cell training
Support concentration.
On the basis of such scheme, described cell culture bags are incubated in cell culture incubator, described cell culture
Case is CO2Cell culture incubator.
Beneficial effect：
1st, the cell culture bags employed in this cell culture system, safe, simple in construction, reasonable in design, use
It is convenient, by setting cell culture bags and cell culture medium storage bag, extremely safe ready during use, each cell culture bags it
Between when needing to be attached using sterile heat seal bulb-tubulating machine heat seal connect, reduce cell contamination and operating personnel infection it is several
Rate, the security of operation is improved, improve the operating efficiency of cell culture, can be exclusively used in cell culture incubator.
2nd, the cell culture bags of the system are trained using the ventilative material for meeting plastics Aqueous injection container standard in cell
Without stripping material during supporting, without to the dysgenic plasticizer of cell generation or stripping material；Using the poly- of highly-breathable
Ethene, TPX or EVA etc., gas concentration environmental requirement and the requirement of pH value during cell culture can be met；Connected using connecting tube
Connect, when injecting cell culture medium or sampling, by direct operating of contacts, improve the security of operation；With using culture
Bottle is compared, and gas exchange area is big, beneficial to the mass propgation in limited culture space, can obtain greater number of cell, envelope
Enclosed culture, reduce cell contamination and the probability of operating personnel's infection；The material transparent flexible of cell culture bags, it is easy to aobvious
The state of cell is directly observed under micro mirror.
The key problem in technology point and point to be protected of the utility model：
1st, the utility model is mainly a kind of high efficiency training of the mass cell production and culture used under conventional environment
The system of supporting, including cell culture bags and cell culture medium storage bag, each bag is separate, and each bag has different specifications.
2nd, cell culture bags are connected with three connections using the ventilative material for meeting plastics Aqueous injection container standard
Pipe, respectively the first connecting tube, the second connecting tube and the 3rd connecting tube, cell culture medium storage bag are airtight material, connection
4th connecting tube, the 5th connecting tube.
3rd, usually for separate state, it is necessary to be protected when carrying out nutrient solution infusion using sterile heat seal bulb-tubulating machine between each bag of body
Demonstrate,prove and heat seal connection is carried out under germ-free condition, is disconnected.
4th, trained in cell culture by the culture density according to different cells from different size of cell culture bags
Support, to ensure the optium concentration of cell growth.
5th, cell culture medium storage bag disposably volume can prepare cell culture medium, be finished in one week, without at any time
Prepare, scale be present on cell culture medium storage bag body.
The cell category of 6 cell culture system cultures includes suspension cell and attached cell.
Brief description of the drawings
The utility model has drawings described below：
The structure chart of Fig. 1 cell culture bags.
The structure chart of Fig. 2 cell culture medium storage bags.
Embodiment
The utility model is described in further detail below in conjunction with attached Fig. 1 and 2.
A kind of production of mass cell and the high efficiency culture systems of culture, including at least one cell culture bags and at least
One cell culture medium storage bag；
The cell culture bags are used for cell culture, and cell culture bags have different specifications, the cell culture
Connecting tube is interval with the lower end sealing margin of bag, the connecting tube includes the first connecting tube 1, the second connecting tube 2 and the 3rd
Connecting tube 3, the first connecting tube 1 are used to be transfused culture medium, and the second connecting tube 2 is used for the output of cell, and the 3rd connecting tube 3 is used for thin
The input of born of the same parents；
The cell culture medium storage bag is used to store the cell culture medium prepared, is that the cell culture of cell culture bags is defeated
Cell culture medium is noted, cell culture medium storage bag has different specifications, the lower end sealing strip of the cell culture medium storage bag
Connecting tube is provided with edge, the connecting tube includes the 4th connecting tube 4, the 5th connecting tube 5, the 4th connecting tube 4, the 5th connecting tube 5
For to cell culture bags infused cells culture medium；
First connecting tube 1, the second connecting tube 2, the 3rd connecting tube 3, the 4th connecting tube 4, the mouth of pipe of the 5th connecting tube 5
Sealed using heat seal mode.
On the basis of such scheme, described cell culture bags comprise at least the first cell culture bags and the second cell is trained
Bag is supported, when the connecting tube of the first described cell culture bags is connected with the connecting tube of the second described cell culture bags, using connecing
Tube method connects；
When the connecting tube of described cell culture bags is connected with the connecting tube of described cell culture medium storage bag, using connecing
Tube method connects,
Described pipe connection method connection, carries out heat seal connection by sterile heat seal mode or earnestly disconnects, pass through high temperature moment
Operation ensures sterile contamination.
On the basis of such scheme, the second connecting tube 2 of the first described cell culture bags and the second described cell
During the 3rd connecting tube 3 connection of culture bag, connected using pipe connection method；
First connecting tube 1 of described cell culture bags and the 4th connecting tube 4 of described cell culture medium storage bag or
When 5th connecting tube 5 connects, connected using pipe connection method.
On the basis of such scheme, the specification of the cell culture bags includes 50ml, 200ml, 2000ml, 5000ml,
The specification of cell culture medium storage bag includes 50ml, 100ml, 200ml, to ensure to maintain suitable cell in cell cultivation process
Concentration is cultivated, and designs different size cell culture bags and cell culture medium storage bag, in cell cultivation process, by thin
Culture medium is added in born of the same parents' culture medium storage bag or changes the cell culture bags of bigger specification to maintain suitable cell culture concentration,
According to the demand of different cell seeding density, using the cell culture bags and cell culture medium storage bag of dimension size.
On the basis of such scheme, the cell culture medium storage bag body is provided with scale, is conveniently adjusted infusion culture
Base unit weight.
On the basis of such scheme, the material of the cell culture bags and connecting tube is held to meet plastics Aqueous injection
The highly-breathable material of device standard.
On the basis of such scheme, the highly-breathable material can select polyethylene, TPX or EVA etc..
On the basis of such scheme, the cell culture medium storage bag is made up of the airtight material of high barrier, because
It is made of air-locked material, can effectively ensure that the safety asepsis environment of culture medium, disposably volume can be prepared.
The application process of the high efficiency culture systems of the mass cell production and culture, comprises the following steps：
1st, cell culture medium is prepared, cell culture medium is placed in cell culture medium storage bag, is disposably prepared multiple thin
Born of the same parents' culture medium storage bag, it is stand-by that cell culture medium storage bag is positioned over cold storage in 4 DEG C of environment；Cell culture will be housed during use
The cell culture medium storage bag of base is warming up to 37 DEG C；
2nd, the cell of required culture is injected in cell culture bags by the 3rd connecting tube 3, while will be thin using pipe connection method
The 4th connecting tube 4 or the 5th connecting tube 5 of born of the same parents' culture medium storage bag pass through sterile heat seal bulb-tubulating machine and cell culture bags first
The heat seal of connecting tube 1 is connected, and cell culture medium is injected into cell culture bags by extruding, after cell culture medium infused, adopted
The 4th connecting tube 4 or the 5th connecting tube 5 and the first connecting tube 1 are blocked with sterile heat seal bulb-tubulating machine high temperature moment, and the 4th is connected
The mouth of pipe heat seal of the connecting tube 5 of adapter 4 or the 5th and the first connecting tube 1, makes cell culture bags and cell culture medium storage bag
Separation；
3rd, in cell cultivation process, the measure of cell culture concentration is carried out by turbidimetry, when cell culture is not suitable
, it is necessary to add note cell culture medium when preferably cultivating concentration, cell culture medium storage bag is taken, using sterile heat seal bulb-tubulating machine by cell
The heat seal of the first connecting tube 1 that the 4th connecting tube 4 or the 5th connecting tube 5 of the connection of culture medium storage bag are connected with cell culture bags connects
Connect, can effectively ensure that in an aseptic environment, the cell culture medium in cell culture medium storage bag is extruded to cell culture bags
In；
4th, after cell culture medium infused, using sterile heat seal bulb-tubulating machine high temperature moment block the 4th connecting tube 4 or
5th connecting tube 5 and the first connecting tube 1, and by the mouth of pipe heat seal of the 4th connecting tube 4 or the 5th connecting tube 5 and the first connecting tube 1
Sealing, makes cell culture bags be separated with cell culture medium storage bag；
5th, as incubation time increases in cell cultivation process, when cell culture bags, can not to ensure that cell is suitably cultivated dense
It is thin by what is cultivated in archaeocyte culture bag by sterile heat seal bulb-tubulating machine, it is necessary to change the cell culture bags of bigger specification when spending
Born of the same parents are transferred in the bigger cell culture bags of specification by the second connecting tube 2, continue to cultivate；
6th, after the completion of the cell transfer cultivated in archaeocyte culture bag, by sterile heat seal bulb-tubulating machine by archaeocyte culture bag
Separated with existing cell culture bags.
On the basis of such scheme, the cell culture medium includes cell production factor, serum free medium and basis
Culture medium.
On the basis of such scheme, the cell category of the cell culture includes suspension cell and attached cell, described
Suspension cell species includes immunocyte, and attached cell species includes mescenchymal stem cell and IPS and its induction differentiation stem cell.
On the basis of such scheme, the cell culture concentration in the cell culture bags, it is determined using turbidimetry,
By using spectrophotometric determination cell culture fluid absorbance, cell concentration standard curve is contrasted to determine described cell training
Support concentration.
On the basis of such scheme, described cell culture bags are incubated in cell culture incubator, described cell culture
Case is CO2Cell culture incubator.
The cell concentration of culture is judged in cell cultivation process by turbidimetry, (early stage uses the cellular processes of standard
Complete cell concentration standard curve, cell culture medium addition known by curve) know the cultivation conditions of cell,
After determining cultivation conditions, by adjust cell culture medium number (infused cells culture medium) and cell culture bags size (more
Change cell culture bags) ensure cell culture concentration in incubation, ensure optimal culture effect.
Embodiment 1：
The plastic material of the highly-breathable suitable for cell culture is chosen, in the way of Fig. 1, is prepared as cell culture
Bag, by the way of high frequency heat seal, connecting tube is welded in the lower end of cell culture bags, wherein the second connecting tube 2 is used for cell
Output to collect；First connecting tube 1 is used to be transfused culture medium；3rd connecting tube 3 is used for the input of cell.
Embodiment 2：
High barrier is chosen, air-locked plastic material, in the way of Fig. 2, cell culture medium storage bag is prepared as, adopts
With the mode of high frequency heat seal, connecting tube is welded in the lower end of cell culture medium storage bag, wherein the 4th connecting tube the 4, the 5th connects
Adapter 5 is used for cell culture bags infused cells culture medium；Cell culture medium storage bag body is provided with scale.
Embodiment 3
Can be under open environment, using sterile heat seal bulb-tubulating machine, by the first connecting tube 1 of cell culture bags and cell culture
After 4th connecting tube 4 of base storage bag or the heat seal of the 5th connecting tube 5 connection, fresh cell culture medium is injected into cell culture
Bag, carry out cell amplification cultivation.
The content not being described in detail in this specification belongs to prior art known to professional and technical personnel in the field.
Claims (4)
1. a kind of mass cell production and the high efficiency culture systems of culture, it is characterised in that：Trained including at least one cell
Support bag and at least one cell culture medium storage bag；
The cell culture bags are used for cell culture, and connecting tube is interval with the lower end sealing margin of the cell culture bags,
The connecting tube includes the first connecting tube, the second connecting tube and the 3rd connecting tube, and the first connecting tube is used to being transfused culture medium, and second
Connecting tube is used for the output of cell, and the 3rd connecting tube is used for the input of cell；
The cell culture medium storage bag is used to store the cell culture medium prepared temporarily, is cell culture bags infused cells culture
Base, at least one connecting tube is provided with the lower end sealing margin of the cell culture medium storage bag, and the connecting tube includes the 4th
Connecting tube, the 5th connecting tube, the 4th connecting tube, the 5th connecting tube are used for cell culture bags infused cells culture medium；
First connecting tube, the second connecting tube, the 3rd connecting tube, the 4th connecting tube, the mouth of pipe of the 5th connecting tube use heat seal
Sealing means seal.
2. mass cell production as claimed in claim 1 and the high efficiency culture systems of culture, it is characterised in that：
Described cell culture bags comprise at least the first cell culture bags and the second cell culture bags, the first described cell culture
When the connecting tube of bag is connected with the connecting tube of the second described cell culture bags, connected using pipe connection method；Described cell culture
When the connecting tube of bag is connected with the connecting tube of described cell culture medium storage bag, connected using pipe connection method；Or
When second connecting tube of the first described cell culture bags is connected with the 3rd connecting tube of the second described cell culture bags,
Connected using pipe connection method；First connecting tube of described cell culture bags is connected with the 4th of described cell culture medium storage bag
When pipe or the 5th connecting tube connect, connected using pipe connection method.
3. mass cell production as claimed in claim 1 and the high efficiency culture systems of culture, it is characterised in that：Described
The specification of cell culture bags includes 50ml, 200ml, 2000ml, 5000ml, the specification of cell culture medium storage bag include 50ml,
100ml, 200ml, cell culture medium storage bag body are provided with scale, are conveniently adjusted infusion culture base unit weight.
4. mass cell production as claimed in claim 1 and the high efficiency culture systems of culture, it is characterised in that：It is described thin
The material of born of the same parents' culture bag and connecting tube is to meet the highly-breathable material of plastics Aqueous injection container standard；The highly-breathable
Material includes polyethylene, TPX and EVA；The cell culture medium storage bag is made up of the airtight material of high barrier.
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| WO2018137428A1 (en) * | 2017-06-06 | 2018-08-02 | 北京呈诺医学科技有限公司 | High efficiency culturing system for large-scale cell production and culturing, and application method |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| WO2018137428A1 (en) * | 2017-06-06 | 2018-08-02 | 北京呈诺医学科技有限公司 | High efficiency culturing system for large-scale cell production and culturing, and application method |
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| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CB03 | Change of inventor or designer information | ||
| CB03 | Change of inventor or designer information |
Inventor after: Gu Yuchun Inventor before: Gu Yuchun Inventor before: Liu Jianqiang |
|
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20190919 Address after: 102600 Beijing Daxing District, Beijing Economic and Technological Development Zone, No. 13 Building, 18 Kechuang Thirteenth Street, No. 3, 301 Patentee after: National Health Chengnuo Biotechnology (Beijing) Co., Ltd. Address before: 100008 North Fourth Ring Road, Haidian District, Haidian District, Beijing, 2106 Patentee before: Beijing promise medical science and Technology Co Ltd |