CN204417517U - A kind of mass cell cultivates multiple-bag - Google Patents
A kind of mass cell cultivates multiple-bag Download PDFInfo
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- CN204417517U CN204417517U CN201520006619.3U CN201520006619U CN204417517U CN 204417517 U CN204417517 U CN 204417517U CN 201520006619 U CN201520006619 U CN 201520006619U CN 204417517 U CN204417517 U CN 204417517U
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- bag
- collecting bag
- sample sack
- dropping funnel
- pipe connecting
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Abstract
The utility model relates to biomedical engineering and technical field of medical examination, specifically a kind of mass cell cultivates multiple-bag, comprise culture bag body, sample sack system and collecting bag system, the side of described culture bag body is communicated with sample sack Link Port, collecting bag Link Port, raw material adding mouth, feed supplement adding mouth successively and changes liquid filters mouth, the sample sack that described sample sack system comprises sample sack pipe connecting and is connected on sample sack pipe connecting, sample sack Link Port is communicated with described sample sack pipe connecting; The collecting bag that described collecting bag system comprises collecting bag pipe connecting and is connected on collecting bag pipe connecting.The beneficial effects of the utility model are, structure is simple, easy for operation, good stability, reliability are high, simple operation, be conducive to the cultivation of cell and obtain more how high-quality cell, directly can carry out the multiple operations such as microscopic examination, cell amplification, sampling and collection can be realized in closed system, improve cell culture efficiency and reduce pollution rate.
Description
Technical field
The utility model relates to biomedical engineering and technical field of medical examination, and specifically a kind of mass cell cultivates multiple-bag.
Background technology
The tissue taken out in living animal body or cell to be cultivated and physiological condition in analogue body by cell cultures general reference in vitro, to realize continued propagation and the amplification of cell.Cell cultures is the basic condition realizing Growth of Cells, is widely used in the fields such as biology, medical science and pharmacy.When current scientific research field carries out cell cultures, the culturing bottle adopting PET or PET6 material, uses, belongs to medical disposable material more after Gamma gamma ray sterilization.But, because culturing bottle belongs to open system, can not full closeding state be realized, not reach GMP standard, not meet clinical treatment specification.And application cell culture bag can realize airtight cell culture system, in GMP laboratory, reliable cell derived can be provided for clinical.
Application number is " 201320010782.8 ", Authorization Notice No. is " CN 203112848 U ", name is called that the Chinese utility model patent of " a kind of Polygons plastic culture bag of cell cultures " discloses a kind of Polygons cell culture bags, its composition comprises: Polygons plastics bag egative film, described Polygons plastics bag egative film welding plastic bag upper slice, described plastics bag upper slice has inlet mouth and opening for feed, described Polygons plastics bag egative film is regular hexagon, octagon or dodecagon.The defect of this utility model patent is: do not keep sample bag, can not monitor the quality and quantity of cell in culture bag; There is no cell harvesting bag, can only disposable collecting cultured cells; Do not change liquid system, cultured cells can not be changed liquid and be supplemented new substratum.
Application number is " 200980110585.X ", Authorization Notice No. is " CN 101978041 B ", name is called that the Chinese invention patent of " passage restricted multi-layer cellular culture systems " discloses a kind of multi-layer cellular culture apparatus, it comprises: a. at least three cell culture chambers, and each cell culture chamber has at least one port; B. at least Liang Ge globality conduit room; With c. sliding end lid, described sliding end lid is constructed and arranged to engage with at least one port of at least three cell culture chambers of described multi-layer cellular culture apparatus, and port that is open or that close is provided, wherein, passage that is open or that close determines in the following manner: by this port cap relative at least one port described with release position or detent position slip joint, wherein, the port cap of release position comprises strainer, and the port cap of detent position comprises partition; Described sliding end lid is connected to described multi-layer cellular culture apparatus by hinged junctor.The defect of this patent of invention is: complex structure, and product cost is high, non-once consumptive material, is applicable to the cultivation of attached cell, is not suitable for the needs of clinical cytology treatment.
Utility model content
The purpose of this utility model is to overcome the deficiencies in the prior art, provides a kind of fully closed scientific and reasonable, easy to use, that culture effect is good mass cell to cultivate and collection multiple-bag.
In order to achieve the above object, the utility model provides a kind of mass cell and cultivates multiple-bag, comprise culture bag body, sample sack system and collecting bag system, the side of described culture bag body is communicated with sample sack Link Port, collecting bag Link Port, raw material adding mouth, feed supplement adding mouth successively and changes liquid filters mouth, wherein:
The sample sack that described sample sack system comprises sample sack pipe connecting and is connected on sample sack pipe connecting, sample sack Link Port is communicated with described sample sack pipe connecting;
The collecting bag that described collecting bag system comprises collecting bag pipe connecting and is connected on collecting bag pipe connecting, collecting bag Link Port is communicated with described collecting bag pipe connecting.
Preferably, described raw material adding mouth connects raw cell bag and culture bag by aseptic bulb-tubulating machine, will treat that cultured cells proceeds in cell culture bags body.
Preferably, described feed supplement adding mouth stretches out the first dropping funnel, sheathed first dropping funnel plug on the first dropping funnel;
Described first dropping funnel and the first dropping funnel plug are demountable structure.
Preferably, the first dropping funnel on described feed supplement adding mouth realizes adding nutritional factor or cytokine in cell culture bags body by connecting syringe.
Preferably, the described liquid that changes filters on mouth and stretches out the second dropping funnel, sheathed second dropping funnel plug on the second dropping funnel;
Axially two-layer filtering membrane is provided with successively along it in described second dropping funnel;
Described second dropping funnel and the second dropping funnel plug are demountable structure.
Preferably, the filtering membrane near the second dropping funnel plug in the second described dropping funnel is 70um cell filtration film; Filtering membrane away from the second dropping funnel plug in second dropping funnel is 3um cell filtration film.
Preferably, described liquid the second dropping funnel filtered on mouth that changes passes through to connect vacuum extractor to realize changing liquid to carrying out long-time cultivation in cell culture bags body.
Preferably, described 70um cell filtration film is nylon membrane, blocks filtering system in order to prevent large cell mass.
Preferably, described 3um cell filtration film is transwell film, in order to prevent from cultured cells being siphoned away when vacuum suction.
Preferably, described collecting bag is provided with collecting bag and detects sample bag;
Described collecting bag and collecting bag detect sample bag and are communicated with by detecting sample bag pipe connecting.
Preferably, described culture bag body is integral type structure.
Preferably, described culture bag body adopts heat seal mode to form sealing the margin.
Preferably, described multiple-bag adopts plastic material hot synthetic type to make, and preferably adopts ethylene-vinyl acetate copolymer (EVA).EVA is transparent finish plate, has good flexibility and elasticity, and its chemical stability, resistance to elevated temperatures are good, do not have biology, chemically reactive, nontoxicity and molding processibility is good, can carry out autoclaving at 200 DEG C, can O thoroughly
2, CO
2, ultraviolet, visible ray and infrared rays, but waterproof, anti-aging and resistance to ozone intensity is good.Toughness can be kept in liquid nitrogen, be applicable to apparatus.
Preferably, described sample sack Link Port, collecting bag Link Port, raw material adding mouth, feed supplement adding mouth and change liquid filter mouth be round tubular structure;
Described sample sack Link Port, collecting bag Link Port, raw material adding mouth, feed supplement adding mouth and change liquid to filter the axis of mouth identical.
Preferably, described multiple-bag has 1 ~ 5 sample sack, and described sample sack is of a size of 5 × 5cm;
Described multiple-bag has 1 ~ 5 collecting bag, and described collecting bag is of a size of 10 × 15cm;
Preferably, described multiple-bag has 3 sample sacks.
Preferably, described multiple-bag has 3 collecting bags.
Preferably, described sample sack Link Port, collecting bag Link Port, raw material adding mouth, feed supplement adding mouth and change liquid and filter on mouth and be configured with medical folder respectively;
Be configured with medical between described sample sack pipe connecting and sample sack to press from both sides;
Be configured with medical between described collecting bag pipe connecting and collecting bag to press from both sides.
Preferably, be configured with medical between described collecting bag and detection sample bag pipe connecting to press from both sides.
The beneficial effects of the utility model are:
1, can the gas molecule such as penetrating oxygen and carbonic acid gas by culture bag body, and the microorganisms such as bacterium, preventing pollution can not be completely cut off permeable, simultaneously, gas exchange area is larger, be conducive to carrying out a large amount of cell cultures in limited space, more how high-quality cell can be obtained;
2, the utility model can be applied to the aspects such as cell cultures, cell amplification, cell freezing, transgenosis, continuous perfusion culture and antibody generation, ensures omnidistance sterile state, effectively reduces the probability of cell contamination and operator's infection;
3, bag chooses clear flexible material, is convenient under the microscope directly observation of cell state and supervision cell cultivation process;
4, concentration and the vigor of dynamic monitoring culture bag inner cell is carried out different incubation time can be realized in closed system under.In cell cultivation process, by heat-sealing machine and the sampling of aseptic bulb-tubulating machine, fluid infusion, change liquid, can ensure all pipeline not openings, the airtight culture systems formed by means of only the gas molecule such as oxygen and carbonic acid gas prevents bacterium and microorganism from polluting.Medical folder of sample sack Link Port will be opened after cell mixing in culture bag body, medical folder is closed after clamp-oning appropriate cell, reusable heat closes machine and the sample sack Link Port heat seal of culture bag body is cut off, and can detect cell in sample sack and not destroy original closed system;
5, the continuation not affecting remaining cell in cell culture bags while collecting culturing cell at times under can realizing different incubation time in closed system is cultivated.By cell mixing in culture bag body, open medical folder of collecting bag Link Port, the cell concn that detects of bag per sample, converse the milliliter number needing the cell quantity collected, medical folder is closed after extruding the cell of corresponding number, after the heat seal of collecting bag Link Port being cut off with heat-sealing machine, for clinical treatment.Cell in collecting bag can be used for product Quality Control, and remaining cell can continue to cultivate in culture bag body.Connect the collecting bag of different quantities as required, general preferably 3, comparatively meet the cycle of clinical cytology treatment.As cytotoxic T cell carries out immunotherapy, transferred in cell culture bags by the cell of collection after cultivating 2 weeks, point 3 collection cells also feed back, each 1 ~ 3 × 10
9individual CIK cell;
6, feed supplement application of sample pipe realizes adding nutritional factor or cytokine in cell cultivation process by connecting syringe; Change liquid strainer tube and be communicated with replaceable nutrient solution to realize changing liquid in culturing process.
7, the utility model structure is simple, easy for operation, good stability, reliability are high, simple operation, be conducive to the cultivation of cell and obtain more how high-quality cell, and directly can carry out the multiple operations such as microscopic examination, ensure omnidistance aseptic, cell amplification, sampling and collection can be realized in closed system, greatly improve cell culture efficiency and reduce pollution rate, being applicable to suspension cell and increasing in a large number and clinical cytology treatment.
Accompanying drawing explanation
Fig. 1 is structural representation of the present utility model;
Wherein:
1-culture bag body 2-sample sack system 21-sample sack pipe connecting
22-sample sack 3-collecting bag system 31-collecting bag pipe connecting
32-collecting bag 33-collecting bag detects sample bag 41-first dropping funnel
42-first dropping funnel plug 51-second dropping funnel 52-second dropping funnel plug
Embodiment
Below in conjunction with the drawings and specific embodiments, the utility model is described further.
Embodiment 1:
A kind of mass cell as shown in Figure 1 cultivates multiple-bag, comprise culture bag body 1, sample sack system 2 and collecting bag system 3, the side of described culture bag body 1 is communicated with sample sack Link Port, collecting bag Link Port, raw material adding mouth, feed supplement adding mouth successively and changes liquid filters mouth, wherein:
Described sample sack system 2 comprises sample sack pipe connecting 21 and is connected to the sample sack 22 on sample sack pipe connecting 21, and sample sack Link Port is communicated with described sample sack pipe connecting 21;
Described collecting bag system 3 comprises collecting bag pipe connecting 31 and is connected to the collecting bag 32 on collecting bag pipe connecting 31, and collecting bag Link Port is communicated with described collecting bag pipe connecting 31.
Described raw material adding mouth connects raw cell bag and culture bag by aseptic bulb-tubulating machine, will treat that cultured cells proceeds in cell culture bags body 1.
Described feed supplement adding mouth stretches out sheathed first dropping funnel plug 42 on the first dropping funnel 41, first dropping funnel 41;
Described first dropping funnel 41 and the first dropping funnel plug 42 are demountable structure.
The first dropping funnel 41 on described feed supplement adding mouth realizes adding nutritional factor or cytokine in cell culture bags body 1 by connecting syringe.
Sheathed second dropping funnel plug 52 on the second dropping funnel 51, second dropping funnel 51 is stretched out in described changing on liquid filtration mouth;
Axially two-layer filtering membrane is provided with successively along it in described second dropping funnel 51;
Described second dropping funnel 51 and the second dropping funnel plug 52 are demountable structure.
Filtering membrane near the second dropping funnel plug 52 in the second described dropping funnel 51 is 70um cell filtration film; Filtering membrane away from the second dropping funnel plug 52 in second dropping funnel 51 is 3um cell filtration film.
Described liquid the second dropping funnel 51 filtered on mouth that changes passes through to connect vacuum extractor to realize changing liquid to carrying out long-time cultivation in cell culture bags body 1.
Described 70um cell filtration film is nylon membrane, blocks filtering system in order to prevent large cell mass.
Described 3um cell filtration film is transwell film, in order to prevent from cultured cells being siphoned away when vacuum suction.
Described collecting bag 32 is provided with collecting bag and detects sample bag 33;
Described collecting bag 32 and collecting bag detect sample bag 33 and are communicated with by detecting sample bag pipe connecting.
Described culture bag body 1 is integral type structure.
Described culture bag body 1 adopts heat seal mode to form sealing the margin.
Described multiple-bag adopts plastic material hot synthetic type to make, and preferably adopts ethylene-vinyl acetate copolymer (EVA).EVA is transparent finish plate, has good flexibility and elasticity, and its chemical stability, resistance to elevated temperatures are good, do not have biology, chemically reactive, nontoxicity, and molding processibility are good.Autoclaving can be carried out at 200 DEG C, can O thoroughly
2, CO
2, ultraviolet, visible ray and infrared rays, but waterproof, anti-aging and resistance to ozone intensity is good.Toughness can be kept in liquid nitrogen, be applicable to apparatus.
Described sample sack Link Port, collecting bag Link Port, raw material adding mouth, feed supplement adding mouth and change liquid filter mouth be round tubular structure;
Described sample sack Link Port, collecting bag Link Port, raw material adding mouth, feed supplement adding mouth and change liquid to filter the axis of mouth identical.
Described multiple-bag has 1 ~ 5 sample sack 22, more preferably gets 3, and described sample sack 22 is of a size of 5 × 5cm;
Described multiple-bag has 1 ~ 5 collecting bag 32, more preferably gets 3, and described collecting bag 32 is of a size of 10 × 15cm;
Described sample sack Link Port, collecting bag Link Port, raw material adding mouth, feed supplement adding mouth and change liquid and filter on mouth and be configured with medical folder respectively;
Be configured with medical between described sample sack pipe connecting 21 and sample sack 22 to press from both sides;
Be configured with medical between described collecting bag pipe connecting 31 and collecting bag 32 to press from both sides.
Be configured with medical between described collecting bag 32 and detection sample bag pipe connecting to press from both sides.
Described mass cell cultivation multiple-bag structure is simple, easy for operation, good stability, reliability are high, simple operation, be conducive to the cultivation of cell and obtain more how high-quality cell, and directly can carry out the multiple operations such as microscopic examination, ensure omnidistance aseptic, cell amplification, sampling and collection can be realized in closed system, greatly improve cell culture efficiency and reduce pollution rate, being applicable to suspension cell and increasing in a large number and clinical cytology treatment.
Below the preferred embodiment that the utility model is created is illustrated, but the utility model is created and is not limited to described embodiment, those of ordinary skill in the art can also make all equivalent modification or replacement under the prerequisite without prejudice to the utility model creative spirit, and these equivalent modifications or replacement are all included in the application's claim limited range.
Claims (10)
1. a mass cell cultivates multiple-bag, it is characterized in that: comprise culture bag body (1), sample sack system (2) and collecting bag system (3), the side of described culture bag body (1) is communicated with sample sack Link Port, collecting bag Link Port, raw material adding mouth, feed supplement adding mouth successively and changes liquid filters mouth, wherein:
Described sample sack system (2) comprises sample sack pipe connecting (21) and is connected to the sample sack (22) on sample sack pipe connecting (21), and sample sack Link Port is communicated with described sample sack pipe connecting (21);
Described collecting bag system (3) comprises collecting bag pipe connecting (31) and is connected to the collecting bag (32) on collecting bag pipe connecting (31), and collecting bag Link Port is communicated with described collecting bag pipe connecting (31).
2. a kind of mass cell according to claim 1 cultivates multiple-bag, it is characterized in that: described feed supplement adding mouth stretches out the first dropping funnel (41), the upper sheathed first dropping funnel plug (42) of the first dropping funnel (41);
Described first dropping funnel (41) and the first dropping funnel plug (42) are demountable structure.
3. a kind of mass cell according to claim 1 cultivates multiple-bag, it is characterized in that: described changing on liquid filtration mouth stretches out the second dropping funnel (51), the upper sheathed second dropping funnel plug (52) of the second dropping funnel (51);
Axially two-layer filtering membrane is provided with successively along it in described second dropping funnel (51);
Described second dropping funnel (51) and the second dropping funnel plug (52) are demountable structure.
4. a kind of mass cell according to claim 3 cultivates multiple-bag, it is characterized in that: the filtering membrane near the second dropping funnel plug (52) in described the second dropping funnel (51) is 70um cell filtration film; Filtering membrane away from the second dropping funnel plug (52) in second dropping funnel (51) is 3um cell filtration film.
5. a kind of mass cell according to claim 1 cultivates multiple-bag, it is characterized in that: described collecting bag (32) is provided with collecting bag and detects sample bag (33);
Described collecting bag (32) and collecting bag detect sample bag (33) and are communicated with by detecting sample bag pipe connecting.
6. a kind of mass cell according to claim 1 cultivates multiple-bag, it is characterized in that: described culture bag body (1) is integral type structure.
7. a kind of mass cell according to claim 1 cultivates multiple-bag, it is characterized in that: described sample sack Link Port, collecting bag Link Port, raw material adding mouth, feed supplement adding mouth and change liquid and filter mouth and be round tubular structure;
Described sample sack Link Port, collecting bag Link Port, raw material adding mouth, feed supplement adding mouth and change liquid to filter the axis of mouth identical.
8. a kind of mass cell according to claim 1 cultivates multiple-bag, it is characterized in that: described multiple-bag has 1 ~ 5 sample sack (22), and described sample sack (22) is of a size of 5 × 5cm;
Described multiple-bag has 1 ~ 5 collecting bag (32), and described collecting bag (32) is of a size of 10 × 15cm;
9. a kind of mass cell according to claim 1 or 7 cultivates multiple-bag, it is characterized in that: described sample sack Link Port, collecting bag Link Port, raw material adding mouth, feed supplement adding mouth and change liquid and filter on mouth and be configured with medical folder respectively;
Be configured with medical between described sample sack pipe connecting (21) and sample sack (22) to press from both sides;
Be configured with medical between described collecting bag pipe connecting (31) and collecting bag (32) to press from both sides.
10. a kind of mass cell according to claim 5 cultivates multiple-bag, it is characterized in that: be configured with medical between described collecting bag (32) and detection sample bag pipe connecting and press from both sides.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520006619.3U CN204417517U (en) | 2015-01-04 | 2015-01-04 | A kind of mass cell cultivates multiple-bag |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520006619.3U CN204417517U (en) | 2015-01-04 | 2015-01-04 | A kind of mass cell cultivates multiple-bag |
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| CN204417517U true CN204417517U (en) | 2015-06-24 |
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| CN201520006619.3U Expired - Fee Related CN204417517U (en) | 2015-01-04 | 2015-01-04 | A kind of mass cell cultivates multiple-bag |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107236669A (en) * | 2017-06-06 | 2017-10-10 | 北京呈诺医学科技有限公司 | A kind of the high efficiency culture systems and application process of mass cell production and culture |
| CN110305789A (en) * | 2019-07-09 | 2019-10-08 | 上海华颜医药科技有限公司 | One group of immune culture three joint bag and its application |
-
2015
- 2015-01-04 CN CN201520006619.3U patent/CN204417517U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107236669A (en) * | 2017-06-06 | 2017-10-10 | 北京呈诺医学科技有限公司 | A kind of the high efficiency culture systems and application process of mass cell production and culture |
| CN110305789A (en) * | 2019-07-09 | 2019-10-08 | 上海华颜医药科技有限公司 | One group of immune culture three joint bag and its application |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20150624 Termination date: 20170104 |