CN201325964Y - Open circulation type tubular microorganism dialysis cultivation system - Google Patents
Open circulation type tubular microorganism dialysis cultivation system Download PDFInfo
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- CN201325964Y CN201325964Y CNU2008202378306U CN200820237830U CN201325964Y CN 201325964 Y CN201325964 Y CN 201325964Y CN U2008202378306 U CNU2008202378306 U CN U2008202378306U CN 200820237830 U CN200820237830 U CN 200820237830U CN 201325964 Y CN201325964 Y CN 201325964Y
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Abstract
The utility model relates to an open circulation type tubular microorganism dialysis cultivation system, in particular to an open circulation type tubular microorganism dialysis cultivator system. The dialysis cultivator system comprises a sealed hollow cylindrical culture medium chamber, and a sealed hollow cylindrical microorganism dialysis cultivation chamber is arranged inside the culture medium chamber; the upper end and the lower end of the culture medium chamber are respectively connected with a culture medium storage chamber through connecting pipes, and peristaltic pumps are connected on the connecting pipes in series; the upper end and the lower end of the microorganism dialysis cultivation chamber are respectively connected with a culture solution storage chamber through connecting pipes, and peristaltic pumps are connected on the connecting pipes in series; exhaust holes are respectively formed at the upper end of the culture medium chamber and the upper end of the microorganism dialysis cultivation chamber, and exhaust holes are also respectively formed at the upper ends of the culture medium storage chamber and the culture solution storage chamber. The planar dialysis membrane is changed into the tubular dialysis membrane in the dialysis cultivator system, the non-circulation type dialysis cultivation is changed into the circulation type dialysis cultivation, and the single-batch dialysis cultivation method is changed into the continuous multi-batch dialysis cultivation method.
Description
Technical field
The utility model relates to a kind of open type cyclic tubular microbial dialysis incubator system, particularly relates to the specific equipment that is applicable to obligatory anaerobic bacteria and facultative anaerobe microbial dialysis incubator suitability for industrialized production.
Background technology
Microbial dialysis is cultivated can not only significantly improve cell concn, can also obtain the comparatively cell and the macromolecule product of purifying.Since the 1950's, though the report of a large amount of experiment dialysis culturing device success is arranged both at home and abroad, the equipment that can be used for suitability for industrialized production really belongs to exception.China in 1985 is because of development research Clostridium botulinum dialysis culture vaccine, obtains " dialysis culturing device of microorganism " utility model patent certificate that China Intellectual Property Office issues (on October 9th, 1985 No. 1873).Adopting this closed acyclic type dialysis culture device can make C type clostridium botulinum toxin produce malicious ability improves more than 30 times than well-established law; The product poison ability of unit volume substratum increases by 1.8～6 times than well-established law; Toxin purity improves 43 times than well-established law.Its product makes the sheep immunizing dose reduce to 0.2ml by every 4ml of conventional production methods.This method has been listed China's " veterinary biological product production and inspection procedure " in, and it not only improves the quality of products, and reduces production costs, and is that exploitation combined vaccine is truly laid a good foundation.
After this, this dialysis culture technology kills mouse and has opened up new production method for China carries out botulinus toxin again.But the acyclic microbial dialysis culture apparatus of above-mentioned this dead front type, one batch of needs of every use are used saturated vapor autoclaving three times, sterilize as empty van sterilization, medium sterilization and superseded the processing, loading and unloading capacity is very loaded down with trivial details, and every batch with after all need change dialysis membrane, this is incompatible for industrial production requirement.
The utility model content
The utility model purpose is to provide a kind of open type cyclic tubular microbial dialysis cultivation system of being convenient to suitability for industrialized production.
The utility model comprises the hollow cylinder Culture Medium Laboratory of sealing, and the hollow cylinder microbial dialysis culturing room of sealing is set in described Culture Medium Laboratory; The upper and lower end of described shape Culture Medium Laboratory is connected with the substratum storing chamber by communicating pipe respectively, is connected in series peristaltic pump on described pipe connecting; The upper and lower end of described microbial dialysis culturing room is connected with the nutrient solution storing chamber by communicating pipe respectively, is connected in series peristaltic pump on described pipe connecting; In described Culture Medium Laboratory upper end, venting hole is set respectively, venting hole is set respectively in the upper end of substratum storing chamber and nutrient solution storing chamber in microbial dialysis culturing room upper end.
Dialysis culture is meant in the culture environment of semi-permeable membranes, makes the method for microorganism growth.The Culture Medium Laboratory nutritive ingredient sees through semi-permeable membranes and is diffused into the microorganism culturing chamber, and the harmful product of microbial metabolism small molecular weight sees through semi-permeable membranes and is diffused into Culture Medium Laboratory.Microorganism can make full use of nutritive ingredient like this, also improves microorganism culturing chamber growing environment simultaneously, reaches the microbial dialysis culturing purposes with this.
The utility model provides a kind of open type cyclic tubular microbial dialysis incubator system, mainly form the microbial dialysis incubator by tubulose dialysis culture chamber and outside ring-type Culture Medium Laboratory, use peristaltic pump respectively by the pipeline of import and export separately of above-mentioned two Room and link to each other, constitute open type cyclic tubular dialysis culture device system with outside dialysis culture liquid reservoir bottle, liquid medium reservoir bottle.
The utility model is compared with original closed acyclic type microbial dialysis culture apparatus, the plane dialysis membrane is changed into the tubulose dialysis membrane, acyclic type dialysis culture is changed into the circular form dialysis culture, single batch of dialysis culture method changed into open continuous multiple batches of dialysis culture production method, advanced again a crucial step for realizing the dialysis culture suitability for industrialized production.
Superiority of the present utility model shows:
(1) gives full play to the useful area of dialysis membrane, shorten distance between culturing room and the Culture Medium Laboratory, by two Room separately in opposite directions sluggish flow make the dialysis membrane both sides obtain uniform rate of permeation, reach ideal dialysis culture effect with this, improve the yield rate of cell and macromolecule product thereof.
(2) after finishing every batch of microbial dialysis cultivation, by the Culture Medium Laboratory of dialysis culture device outside and the replacing of microorganism culturing chamber, can directly use continuously the dialysis culture device, improve plant factor, the autoclaved number of times of saturated steam of obvious minimizing equipment, save electric energy and water consumption, alleviate productive labor intensity.
The volume ratio of microbial dialysis culturing room described in the utility model and Culture Medium Laboratory is 1: 8～12.Long-term practice by closed acyclic type microbial dialysis culture apparatus, be on 1: 10 the basis at the ratio that continue to keep nutrient solution with the volume of liquid medium, open type cyclic tubular microbial dialysis incubator system will further improve and quicken microorganism growth, produces malicious process, to prevent the not enough superfluous bad phenomenon of nutritive ingredient.
Production and assembly for convenience, Culture Medium Laboratory described in the utility model is made up of hollow cylinder post jamb and upper and lower two lids, the two ends of described post jamb are separately positioned between upper and lower two lids, are mutually permanently connected by double-screw bolt and nut between upper and lower two lids.
For the ease of observing, described post jamb is that transparent material or the material that has visor are made.
For microbial dialysis culturing room is fixed in the Culture Medium Laboratory, described microbial dialysis culturing room is made up of tubulose dialysis membrane and upper and lower two stainless steel cages, and the two ends of described tubulose dialysis membrane are connected on upper and lower two stainless steel cages by fixed ring seals respectively.
In addition, establish the air filtration rod of filter cotton in the utility model is also pegged graft respectively on each venting hole.Purpose is to prevent outside dust, microbiological contamination, influences microorganism culturing.
Description of drawings
Fig. 1 is a kind of structural representation of the present utility model.
Embodiment
As shown in Figure 1, the utility model hollow cylinder Culture Medium Laboratory 1 is made up of hollow cylinder post jamb 101 and upper and lower two lids 102,103, the two ends of post jamb 101 are separately positioned between upper and lower two lids 102,103, are mutually permanently connected by double-screw bolt 104 and nut 105,106 between upper and lower two lids 102,103.Post jamb 101 can adopt transparent material or have the visor stainless material and make.
Hollow cylinder microbial dialysis culturing room 2 is made up of tubulose dialysis membrane 201 and upper and lower two stainless steel cages 202,203, and the two ends of tubulose dialysis membrane 201 are sealedly connected on upper and lower two stainless steel cages 202,203 by set collar 204 respectively.And the lower end of following stainless steel ring 203 is welded on the lower cover 103 of cylindrical Culture Medium Laboratory.
Microbial dialysis culturing room 2 is arranged in the centre of Culture Medium Laboratory 1, and to make the microbial dialysis culturing room 2 and the volume ratio of Culture Medium Laboratory 1 be 1: 8～12.
Rubber consent 107 is offered at upper cover body 102 centers at Culture Medium Laboratory 1, offers venting hole 108 and siphunculus mouth 109 on one side.The siphunculus mouth 110 that is communicated with microbial dialysis culturing room 2 is offered at lower cover 103 centers, and lower cover 103 is offered the siphunculus mouth 111 that is communicated with Culture Medium Laboratory 1 on one side.Connect a rubber plug 3 on the rubber consent 107.
Microorganism culturing chamber 2 nutrient solutions also are connected in series a peristaltic pump 12 by delivery port 110 on output tube 10, input nutrient solution storing chamber 8.Offer two holes in the upper end of last stainless steel cage 204, a hole connecting pipe 9, nutrient solution storing chamber 8 exceed the volumetrical nutrient solution and import microbial dialysis culturing room 2 by pipeline 9 through the centre hole of rubber plug 3 by syphonic effect.Establish the air filtration rod 11 of filter cotton in rubber plug 3, also planting, and another hole by stainless steel cage 204, the lower end that makes air filtration rod 11 is communicated with microbial dialysis culturing room 2 formation.The upper end of nutrient solution storing chamber 8 connects the air filtration rod 14 of establishing filter cotton in one.
Offer venting hole 108 at Culture Medium Laboratory 1 loam cake 102, its upper end connects in one establishes the air filtration rod 15 of filter cotton, and air filtration rod 15 is communicated with Culture Medium Laboratory 1 formation.
The utility model will produce tangible production effect when the microbial dialysis that is used to suit is cultivated.As in D and C type Clostridium botulinum dialysis culture, the toxin virulence can improve 2～3 times, and the energy cultured continuously is tens of inferior.Can alleviate production technique labour intensity greatly, shorten the production cycle, save the energy, improve plant factor, reduce production costs.
Claims (6)
1, open type cyclic tubular microbial dialysis cultivation system is characterized in that comprising the hollow cylinder Culture Medium Laboratory of sealing, and the hollow cylinder microbial dialysis culturing room of sealing is set in described Culture Medium Laboratory; The upper and lower end of described shape Culture Medium Laboratory is connected with the substratum storing chamber by communicating pipe respectively, is connected in series peristaltic pump on described pipe connecting; The upper and lower end of described microbial dialysis culturing room is connected with the nutrient solution storing chamber by communicating pipe respectively, is connected in series peristaltic pump on described pipe connecting; In described Culture Medium Laboratory upper end, venting hole is set respectively, venting hole is set respectively in the upper end of substratum storing chamber and nutrient solution storing chamber in microbial dialysis culturing room upper end.
2, according to the described open type cyclic tubular microbial dialysis cultivation system of claim 1, the volume ratio that it is characterized in that described microbial dialysis culturing room and Culture Medium Laboratory is 1: 8～12.
3, according to the described open type cyclic tubular microbial dialysis cultivation system of claim 1, it is characterized in that described Culture Medium Laboratory is made up of hollow cylinder post jamb and upper and lower two lids, the two ends of described post jamb are separately positioned between upper and lower two lids, are mutually permanently connected by double-screw bolt and nut between upper and lower two lids.
4,, it is characterized in that described post jamb is that transparent material or the metallic substance that has visor are made according to the described open type cyclic tubular microbial dialysis cultivation system of claim 3.
5, according to the described open type cyclic tubular microbial dialysis cultivation system of claim 1, it is characterized in that described microbial dialysis culturing room is made up of tubulose dialysis membrane and upper and lower two stainless steel cages, the two ends of described tubulose dialysis membrane are connected on upper and lower two stainless steel cages by fixed ring seals respectively.
6,, establish the air filtration rod of filter cotton in it is characterized in that on each venting hole, pegging graft respectively according to the described open type cyclic tubular microbial dialysis cultivation system of claim 1.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNU2008202378306U CN201325964Y (en) | 2008-12-18 | 2008-12-18 | Open circulation type tubular microorganism dialysis cultivation system |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNU2008202378306U CN201325964Y (en) | 2008-12-18 | 2008-12-18 | Open circulation type tubular microorganism dialysis cultivation system |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN201325964Y true CN201325964Y (en) | 2009-10-14 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNU2008202378306U Expired - Fee Related CN201325964Y (en) | 2008-12-18 | 2008-12-18 | Open circulation type tubular microorganism dialysis cultivation system |
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| Country | Link |
|---|---|
| CN (1) | CN201325964Y (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103706253A (en) * | 2013-12-16 | 2014-04-09 | 南昌大学 | Large-size efficient and dynamic dialysis method and device thereof |
| CN104560688A (en) * | 2013-10-23 | 2015-04-29 | 中国科学院沈阳应用生态研究所 | Device and method for culturing hybrid microorganisms |
-
2008
- 2008-12-18 CN CNU2008202378306U patent/CN201325964Y/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104560688A (en) * | 2013-10-23 | 2015-04-29 | 中国科学院沈阳应用生态研究所 | Device and method for culturing hybrid microorganisms |
| CN103706253A (en) * | 2013-12-16 | 2014-04-09 | 南昌大学 | Large-size efficient and dynamic dialysis method and device thereof |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20091014 Termination date: 20111218 |