CN203559051U - Device for cultivating mixed microbes - Google Patents
Device for cultivating mixed microbes Download PDFInfo
- Publication number
- CN203559051U CN203559051U CN201320660445.3U CN201320660445U CN203559051U CN 203559051 U CN203559051 U CN 203559051U CN 201320660445 U CN201320660445 U CN 201320660445U CN 203559051 U CN203559051 U CN 203559051U
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- Prior art keywords
- dialysis membrane
- cultivating
- fixing
- tube
- mixed
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- 244000052616 bacterial pathogens Species 0.000 title abstract 3
- 239000012528 membrane Substances 0.000 claims abstract description 42
- 238000000502 dialysis Methods 0.000 claims abstract description 40
- 244000005700 microbiome Species 0.000 claims description 19
- 238000002156 mixing Methods 0.000 claims description 12
- 239000000463 material Substances 0.000 claims description 11
- 229920001296 polysiloxane Polymers 0.000 claims description 7
- 239000001913 cellulose Substances 0.000 claims description 3
- 229920002678 cellulose Polymers 0.000 claims description 3
- 241000894006 Bacteria Species 0.000 abstract description 13
- 230000001580 bacterial Effects 0.000 abstract description 6
- 230000003993 interaction Effects 0.000 abstract description 4
- 238000004519 manufacturing process Methods 0.000 abstract description 3
- 238000000855 fermentation Methods 0.000 description 3
- 230000004151 fermentation Effects 0.000 description 3
- 238000009434 installation Methods 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 238000006243 chemical reaction Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 239000011521 glass Substances 0.000 description 2
- 230000016507 interphase Effects 0.000 description 2
- 230000000813 microbial Effects 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 239000000741 silica gel Substances 0.000 description 2
- 229910002027 silica gel Inorganic materials 0.000 description 2
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N silicium dioxide Chemical compound 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- 239000010935 stainless steel Substances 0.000 description 1
- HNBDQABBWNOTRU-UHFFFAOYSA-N thalline Chemical compound 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C1=CC=[Tl]C=C1 HNBDQABBWNOTRU-UHFFFAOYSA-N 0.000 description 1
- 239000000052 vinegar Substances 0.000 description 1
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- 239000011782 vitamin Substances 0.000 description 1
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Abstract
The utility model relates to the field of cultivating microbes in a mixed way and in particular relates to a device for cultivating mixed microbes. The device comprises a permeable plug, an outer cylinder, an inner pipe, an inner pipe fixing device and a dialysis membrane fixing device, wherein one end of the outer cylinder is closed, the other end of the outer cylinder is in an opening type, one end of the inner pipe is connected with the dialysis membrane fixing device, the inner pipe is fixed in the outer cylinder by virtue of at least one inner pipe fixing device, and the port of the outer cylinder is sealed by the permeable plug. With the adoption of the device, the mixed bacterium cultivating condition can be highly simulated, pure cultures of two target bacterial strains in the mixed cultivation under different cultivating states can be obtained, and the convenience is provided for studying mutual relations among mixed bacteria. The device is simple in the manufacturing, is convenient in use and can be widely applied to interaction relation study under various mixed bacterium conditions.
Description
Technical field
The utility model relates to the field of mixed culture microorganism, is specially a kind of for cultivating the device of mixing microorganisms.
Background technology
Long-term production practice and increasing scientific research show, numerous microbial processes needs the synergy of multiple bacterial strain (or efficient) to complete, not only in fermentation industry field, as fermentation process for vitamin production, food fermentation and wine, vinegar are brewageed etc., in the environmental protection and the bioenergy field that relate to microorganism, also there is bacterial strain mixed culture to improve the reports of conversion and bioaccumulation efficiency more.Along with the widespread use of mixed bacterium culture technique in every field, the mixed bacterium interphase interaction relation of research has become one of study hotspot of domestic and international scientist to improve mixed bacterium conversion (degraded) efficiency.
In the research of hybrid bacterial strain interphase interaction relation, obtain the purebred of aimed strain after interacting through mixed culture and with other bacterial strain, be first to need the problem that solves.Design a kind of can assurance and can highly imitate mixed culture condition, can obtain fast the purebred experimental installation of aimed strain again, could meet the needs that we study mixed bacterium relation.And such device rarely has report.Similarly patent as disclosed in Chinese Patent Application No. 201220446864.2 " use for laboratory anerobe dialysis culturing device " is to utilize semi-permeable membranes as dialysis membrane, and is that this device is unsuitable for studying aerobic bacteria for the dialysis culture of independent anaerobion.The dialysis culturing device complex structure that " microbial dialysis incubator " of Chinese Patent Application No. 200920104149.9 mentioned, cost is higher.Also there is similar patent report abroad, but be all to utilize semi-permeable membranes as dialysis membrane, and complex structure, high expensive.Traditional method that is used for studying the relation between bacterium of mixing is that the nutrient solution of a kind of microorganism is joined in target microorganism nutrient solution after filtration, carries out shake-flask culture.This method is not only difficult to simulate mixed culture condition, and wastes time and energy, and microbiological contamination probability is high.
Utility model content
The utility model technical issues that need to address are to provide a kind of not only simple but also efficiently a kind of for cultivating the device of mixing microorganisms.
For solving above technical problem, the technical scheme that the utility model adopts is:
For cultivating a device for mixing microorganisms, comprise porous plug, urceolus, inner tube, interior pipe fixing device and dialysis membrane fixing device; Urceolus one end is airtight, and the other end is open type, and inner tube one end connects dialysis membrane fixing device, and inner tube is fixed in urceolus by the interior pipe fixing device of at least one, and urceolus open end seals by porous plug.
Described inner tube one end is provided with outside screw, is provided with externally threaded inner tube one end and is connected by engage thread with dialysis membrane fixing device.
Described inner tube is provided with and between externally threaded one end and dialysis membrane fixing device, is provided with successively silicone gasket, dialysis membrane, dialysis membrane fixed network from top to bottom.
Described silicone gasket outside diameter equates with the interior diameter of dialysis membrane fixing device.
Described dialysis membrane is that aperture is at the cellulose filter membrane of 0.22-0.45 μ m.
Described porous plug, urceolus, inner tube, interior pipe fixing device and dialysis membrane fixing device are thermostable transparent material and make.
The utility model has advantages of:
The utility model adopts thermostable transparent material to make, and by after the utility model device sterilising treatment, adds respectively substratum in urceolus and inner tube, makes the substratum liquid level of inner tube higher than outer tube.Inner and outer pipe accesses respectively after different bacterial strains, and owing to having pressure reduction between two pipes, when shaking culture, the bacterial metabolism material in inner tube substratum can be with medium flow to outer tube, and thalline can not pass through.Cultivate and carefully after for some time to take out inner tube, and bacterium in urceolus substratum is the target microorganism of the pure culture obtaining, and this microorganism has been subject to the effect of inner tube microorganism or has affected.The target microorganism of the pure culture obtaining, can be applicable to carry out the comparative analysis of various biological methods and technology, finally reaches the object of understanding two bacterium mutual relationships and effect.
Device of the present utility model, not only can mix bacterium culture condition by altitude simulation, and can obtain at short notice the pure growth of each aimed strain under different cultivation conditions in mixed culture, for mutual relationship between research mixed bacterium is provided convenience.The utility model device is made simple, easy to use, can be widely used in the interaction relationship research under various mixed bacterium conditions.
Accompanying drawing explanation
Fig. 1 is the structural representation of this experimental installation;
Fig. 2 is the dialysis membrane fixing device schematic diagram of this experimental installation;
Wherein: 1, porous plug, 2, outer tube, 3, inner tube, 4, interior pipe fixing device, 5, dialysis membrane fixing device, 6, silicone gasket, 7, dialysis membrane, 8, dialysis membrane fixed network.
Embodiment
Embodiment 1
For cultivating the device of mixing microorganisms, comprise porous plug, urceolus, inner tube, interior pipe fixing device and dialysis membrane fixing device; Urceolus one end airtight (blind end bottom can be round bottom or flat), the other end is open type, and inner tube one end connects dialysis membrane fixing device, and inner tube is fixed in urceolus by the interior pipe fixing device of at least one, and urceolus port seals by porous plug.Described inner tube one end is provided with outside screw, is provided with externally threaded inner tube one end and is connected by engage thread with dialysis membrane fixing device.Described inner tube is provided with and between externally threaded one end and dialysis membrane fixing device, is provided with successively silicone gasket, dialysis membrane, dialysis membrane fixed network from top to bottom.Described silicone gasket outside diameter equates with dialysis membrane fixing device interior diameter.Said apparatus is wrapped and put into Autoclave sterilizing with kraft paper, stand-by.
Wherein, ventilative plug can be plug or gauze sealing; Urceolus material is glass, external diameter 35.00mm, internal diameter 30.00mm, long 200.00mm; Inner tube material is glass, external diameter 15.00mm, internal diameter 10mm, long 120.00mm; Interior pipe fixing device material is high-temperature resistance plastice, and there is breather hole external diameter 35.00mm, internal diameter 30.00mm, centre; Dialysis membrane fixing device material is high-temperature resistance plastice nut, external diameter 20.00mm, internal diameter 18.00mm, mesopore internal diameter 10.00mm, long 20.00mm; Mesoporous silica gel packing ring material is silica gel, external diameter 18.00mm, mesopore internal diameter 10.00mm; Dialysis membrane is the high temperature resistant filter membranes such as material cellulose filter membrane, aperture 0.22 μ m-0.45 μ m diameter 18.00mm; Dialysis membrane fixed network material is stainless steel, diameter 18.00mm.
Claims (6)
1. for cultivating a device for mixing microorganisms, it is characterized in that: comprise porous plug, urceolus, inner tube, interior pipe fixing device and dialysis membrane fixing device; Urceolus one end is airtight, and the other end is open type, and inner tube one end connects dialysis membrane fixing device, and inner tube is fixed in urceolus by the interior pipe fixing device of at least one, and urceolus open end seals by porous plug.
By claimed in claim 1 for cultivating the device of mixing microorganisms, it is characterized in that: described inner tube one end is provided with outside screw, be provided with externally threaded inner tube one end and be connected by engage thread with dialysis membrane fixing device.
By claimed in claim 2 for cultivating the device of mixing microorganisms, it is characterized in that: described inner tube is provided with and between externally threaded one end and dialysis membrane fixing device, is provided with successively silicone gasket, dialysis membrane, dialysis membrane fixed network from top to bottom.
By claimed in claim 3 for cultivating the device of mixing microorganisms, it is characterized in that: described silicone gasket outside diameter equates with the interior diameter of dialysis membrane fixing device.
By claimed in claim 2 for cultivating the device of mixing microorganisms, it is characterized in that: described dialysis membrane is that aperture is at the cellulose filter membrane of 0.22-0.45 μ m.
By claimed in claim 1 for cultivating the device of mixing microorganisms, it is characterized in that: described porous plug, urceolus, inner tube, interior pipe fixing device and dialysis membrane fixing device are thermostable transparent material and make.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201320660445.3U CN203559051U (en) | 2013-10-23 | 2013-10-23 | Device for cultivating mixed microbes |
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| CN201320660445.3U CN203559051U (en) | 2013-10-23 | 2013-10-23 | Device for cultivating mixed microbes |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104560688A (en) * | 2013-10-23 | 2015-04-29 | 中国科学院沈阳应用生态研究所 | Device and method for culturing hybrid microorganisms |
| CN108456626A (en) * | 2018-03-22 | 2018-08-28 | 黄佳立 | A kind of bioengineering swing arm type microculture soil mixing arrangement |
-
2013
- 2013-10-23 CN CN201320660445.3U patent/CN203559051U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104560688A (en) * | 2013-10-23 | 2015-04-29 | 中国科学院沈阳应用生态研究所 | Device and method for culturing hybrid microorganisms |
| CN108456626A (en) * | 2018-03-22 | 2018-08-28 | 黄佳立 | A kind of bioengineering swing arm type microculture soil mixing arrangement |
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