CN2937146Y - Two-way dialyser - Google Patents
Two-way dialyser Download PDFInfo
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- CN2937146Y CN2937146Y CN 200620120442 CN200620120442U CN2937146Y CN 2937146 Y CN2937146 Y CN 2937146Y CN 200620120442 CN200620120442 CN 200620120442 CN 200620120442 U CN200620120442 U CN 200620120442U CN 2937146 Y CN2937146 Y CN 2937146Y
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- dialyser
- dialyzer
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Abstract
The utility model relates to a two-way dialyser made of stainless steel in the shape of rectangle, which comprises three parts with two culture medium storage chambers on the two sides and a germi-culture incubator in the middle, the three parts being separated through dialyzing membranes. The utility model is characterized in that the dialyser can be used for the cultivation of C-type and D-type Clostridium botulinum, B-type Clostridium novyi, and Escherichia coli; that the C-type Clostridium botulinum cultivated by the two-way dialyser can increase the outcome of the toxin by ten to thirty times than routine cultivation; that the two-way dialyser can bring about good effects in the research of the nutritional requirement, physiology, and metabolite of other bacterium; and that the utility model is applicable for microbiological laboratories, bio-pharmaceuticals factories producing vaccine and anti-biosic toxins, and microbiology research organizations.
Description
Technical field
The utility model relates to a kind of device of bacteriotoxin culture technique, particularly the two-way dialyzer of bacteriotoxin cultivation.
Background technology
To be MetchnikoffShi in 1896 etc. pack vibrio cholerae in the semi-permeable membranes bag into dialysis culture the earliest, implants then in the abdominal cavity of animal, and this moment, bacterium can produce toxin and virulence strengthens.Carnot in 1900 and FournierShi put into culturing bottle with dialysis tubing and carry out dialysis culture, can make streptococcus pneumoniae produce toxin, and wentzed in 1949 and SterneShi cultivate order of succession with dialysis tubing, can obtain the toxin of high density.After, dialysis culture technology dialyzer commonly used all is made up of two parts, one is the nutrient media storage chamber, and one is bacteria growth chamber, is separated by semi-permeable membranes between two Room, carry out the diffusion and the exchange of nutritive substance and meta-bolites simultaneously, to obtain required bacterium and product, so be unidirectional dialyzer, we find that unidirectional dialyzer dialysis area is little, exchange of substance is insufficient, and harvest yield is less.
Summary of the invention
The purpose of this utility model is that the unidirectional dialyzer dialysis of solution area is little, and exchange of substance is insufficient, the problem that harvest yield is less.Designed two-way dialyzer for this reason, be used for Clostridium botulinum C type, D type, Nuo Weishi clostridium Type B and the colibacillary cultivation of dust Xi Shi.
Cultivating C type Clostridium botulinum with two-way dialyzer can make toxin output reach 100-300 ten thousand small white mouse lethal quantity toxin (MLD small white mouse/ml), cultivate high 10-30 doubly than routine.
When being used to cultivate D type Clostridium botulinum, can obtain the toxin of 2000-5000 ten thousand MLD small white mouse/ml.
When being used to cultivate the Nuo Weishi clostridium, or the toxin of acquisition 5-10 ten thousand MLD small white mouse/ml.
When being used for cultivating the Ai Jishi intestinal bacteria, can obtain the bacterium liquid of 15,000,000,000 thalline/ml and higher toxin.
In addition, two-way dialyzer is used to study nutritional requirement, physiology and the meta-bolites of other bacterium, and good effect is all arranged.Two-way dialysis has enlarged dialysis face, can make full use of the nutritive substance in the substratum, and harvest yield is also more to be its advantage.Be applicable to Microbiological Lab, bio-pharmaceuticals factory, the research unit of manufacturing vaccine and toxoid and microorganism.
Technology contents of the present utility model is: a kind of two-way dialyzer, make with stainless steel, be shaped as rectangle, it is characterized in that forming by three parts, left and right two parts (chamber, left side and chamber, right side) are the nutrient media storage chamber, middle (middle chamber) is bacteria growth chamber, about two Room and intermediate chamber separate with dialysis membrane; There are equally distributed 24 holes on the right side of chamber, described left side along welding fixed square flange is arranged on the The square flange; Chamber, described right side and chamber, left side mirror-symmetrical have identical structure; The left and right sides of chamber has equally distributed 24 holes equally along a welding fixed square flange is also respectively arranged in described on these two square flanges; A welding fixed tremie pipe is respectively arranged on the going to the bottom of described three chambers (chamber, left side, chamber, right side, middle chamber), a welding fixed feed-pipe is respectively arranged on the upper base.
During cultivation about the substratum of injection proper amt (according to culturing purposes) in two nutrient media storage chambers (chamber, left side and chamber, right side), middle (middle chamber, be culturing room) injection distilled water, after the sterilization, the microbionation that will cultivate is cultivated in middle culture room, small molecules in the substratum of both sides (molecular weight is below 700) material, enter intermediate chamber through dialysis membrane, for the bacterial growth breeding, and the macromolecular substance of thalline of bacterium and generation then can not form the tired increase of multiplicatrix by dialysis membrane, thereby can obtain higher thalline of concentration and toxin, this when containing toadstool liquid and making vaccine and toxinicide, immunity also can be multiplied.
Description of drawings
The two-way dialyzer positive view of Fig. 1
The two-way dialyzer A-A of Fig. 2 face sectional view
The two-way dialyzer B-B of Fig. 3 face sectional view
The two-way dialyzer stereographic map of Fig. 4
Among the figure, the 1st, supporting leg, the 2nd, tremie pipe, the 3rd, middle chamber, the 4th, side room, the 5th, feed-pipe, the 6th, gasket, the 7th, square flange, the 8th, film block, the 9th, bolt, the 10th, dialysis membrane.
Embodiment
The structure of a two-way dialyzer is as follows:
Volume: long 28cm, wide 23cm, high 43cm; Weight: 10 kilograms; Chamber (nutrient media storage chamber), left side: 20 * 10 * 30cm, volume 6000ml; Chamber, right side (nutrient media storage chamber): 20 * 10 * 30cm, volume 6000ml; Intermediate chamber (culturing room): 20 * 3 * 30cm, volume 1800ml; Cubic capacity 13800ml.
As shown in Figure 1, chamber 4, left side is to be made by stainless steel, there are two supporting legs 1 in the bight, below, there is a welding fixed tremie pipe 2 at the middle part of going to the bottom, there is a welding fixed feed-pipe 5 at the upper base middle part, at square flange 7 of its outer, right side welding, equally distributed 24 holes are arranged on square flange 7, its right side wall then is a film block 8.Chamber, right side and chamber, left side mirror-symmetrical, structure is identical.Middle chamber 4 is culturing room, is to be made by stainless steel equally, and a tremie pipe is fixed in the lower bottom part welding, a feed-pipe is fixed in the upper bottom portion welding, respectively weld a square flange in its left and right side outer, equally distributed 24 holes are arranged on square flange, its left and right sides wall then is the film block.During use, three parts of two-way dialyzer (chamber, left side, chamber, right side and middle chamber) are to use 24 groups of bolted to be in the same place.For preventing that nutrient solution from leaking, and respectively is equipped with a cyclic sealing gasket 6 (totally two) between two fastening square flanges.
When two-way dialyzer uses, load onto dialysis membrane in the bacteria growth chamber both sides earlier, use 24 groups of bolt assemblings to be fixed together three parts of two-way dialyzer; Again the leakage fluid dram below the dialyzer is tied with rubber hose; The 3rd step was according to the requirement of experiment an amount of distilled water of packing in inside bacteria growth chamber; The 4th step was at the indoor an amount of substratum of packing into of the nutrient media storage of both sides, and (opening for feed) suitable for reading adds tampon and wrapping, carries out steam sterilizing.Can put into 37 ℃ of greenhouses cultivations the microbionation that will cultivate in inside bacteria growth chamber during sterilization postcooling to 37 ℃.After cultivation finishes, emit the substratum of both sides earlier from leakage fluid dram (tremie pipe), and then collect the bacterium liquid in the bacteria growth chamber.With after the dialyzer autoclaving, washing is got ready time and to be used then.
Claims (3)
1, a kind of two-way dialyzer, make with stainless steel, be shaped as rectangle, it is characterized in that forming by three parts, chamber, left side (4) and chamber, right side (4) of left and right two parts are the nutrient media storage chamber, chamber in the intermediary (3) is a bacteria growth chamber, about two Room (4) and intermediate chamber (3) separate with dialysis membrane (8).
2, two-way dialyzer according to claim 1, the right side that it is characterized in that the chamber, left side is along there being welding fixed square flange (7), and (7) have equally distributed 24 holes on the The square flange; Chamber, described right side and chamber, left side mirror-symmetrical have identical structure; The left and right sides of chamber is along also respectively there being a welding fixed square flange (7) in described, and (7) have equally distributed 24 holes equally on these two square flanges.
3, two-way dialyzer according to claim 1 is characterized in that on the going to the bottom of chamber, described left side (4), chamber, right side (4), middle chamber (3) a welding fixed tremie pipe (2) being arranged respectively, and a welding fixed feed-pipe (5) is respectively arranged on the upper base.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200620120442 CN2937146Y (en) | 2006-06-22 | 2006-06-22 | Two-way dialyser |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200620120442 CN2937146Y (en) | 2006-06-22 | 2006-06-22 | Two-way dialyser |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN2937146Y true CN2937146Y (en) | 2007-08-22 |
Family
ID=38361169
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200620120442 Expired - Lifetime CN2937146Y (en) | 2006-06-22 | 2006-06-22 | Two-way dialyser |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN2937146Y (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102634447A (en) * | 2012-04-18 | 2012-08-15 | 浙江万里学院 | Micro-array dialysis chamber and enrichment culture method using same |
| CN103865751A (en) * | 2012-12-17 | 2014-06-18 | 中国科学院上海生命科学研究院 | Array-type high-throughput microbe separating culturing apparatus |
-
2006
- 2006-06-22 CN CN 200620120442 patent/CN2937146Y/en not_active Expired - Lifetime
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102634447A (en) * | 2012-04-18 | 2012-08-15 | 浙江万里学院 | Micro-array dialysis chamber and enrichment culture method using same |
| CN102634447B (en) * | 2012-04-18 | 2013-09-18 | 浙江万里学院 | Micro-array dialysis chamber and enrichment culture method using same |
| CN103865751A (en) * | 2012-12-17 | 2014-06-18 | 中国科学院上海生命科学研究院 | Array-type high-throughput microbe separating culturing apparatus |
| CN103865751B (en) * | 2012-12-17 | 2015-11-18 | 中国科学院上海生命科学研究院 | A kind of array high throughput separation and Culture antimicrobial device |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CX01 | Expiry of patent term |
Granted publication date: 20070822 |
|
| EXPY | Termination of patent right or utility model |