CN106701547A - Device for collecting gas volatile matters produced by biological metabolism and real-time collection method thereof - Google Patents
Device for collecting gas volatile matters produced by biological metabolism and real-time collection method thereof Download PDFInfo
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- CN106701547A CN106701547A CN201611218248.0A CN201611218248A CN106701547A CN 106701547 A CN106701547 A CN 106701547A CN 201611218248 A CN201611218248 A CN 201611218248A CN 106701547 A CN106701547 A CN 106701547A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/38—Caps; Covers; Plugs; Pouring means
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/48—Holding appliances; Racks; Supports
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/04—Filters; Permeable or porous membranes or plates, e.g. dialysis
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
- C12M37/02—Filters
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/24—Methods of sampling, or inoculating or spreading a sample; Methods of physically isolating an intact microorganisms
Abstract
The invention provides a device for collecting gas volatile matters produced by biological metabolism and a real-time collection method thereof, and belongs to the technical field of biological chemical engineering. The device for collecting the gas volatile matters produced by biological metabolism comprises a main tank body, a gas supply mechanism and an air collector, wherein the main tank body is provided with a culture cavity for culturing microorganisms or other miniature organisms, a support plane is arranged in the culture cavity and is used for supporting a culture dish, the main tank body is provided with a gas outlet and a sampling port, and the sampling port is arranged at the top end of the main tank body; the gas supply mechanism is used for sending sterile air and/or preset special gas into the culture cavity; the air collector is connected with the sampling port, and is used for collecting the volatile molecules produced by the biological metabolism in real time. The device for collecting the gas volatile matters produced by biological metabolism has the advantages that the culture condition is controllable, and the volatile molecules produced by the biological metabolism can be collected in real time when the microorganisms or other miniature organisms grow.
Description
Technical field
The invention belongs to technical field of biochemical industry, it is related to a kind of collection device of the volatile gas of biological metabolism generation
And its real-time collecting method.
Background technology
During biological growth, it is possible to secrete the small molecule of some volatiles.These small molecules reach certain dense
When spending, the physiological status of biology can be changed, or as being combined with organism specific protein acceptor with physical efficiency, from transcriptional level
Regulation genes of interest activity, so as to trigger the physiological activity of cell individual.At present, collection has the volatile point of physiological function
, mainly be placed in closed container for organism or a portion by son, then carries out volatile gas collection.
The content of the invention
The purpose of the present invention is directed to existing technology and there is above mentioned problem, it is proposed that a kind of condition of culture is controllable, can be
When microorganism or other small-sized biologicals grow, the biological metabolism generation of the Real-time Collection of the volatile molecules of metabolism generation is carried out
The collection device and its real-time collecting method of volatile gas.
The purpose of the present invention can be realized by following technical proposal：The collection dress of the volatile gas that biological metabolism is produced
Put, including：
Main tank body, is provided with the closed culture chamber for cultivating microorganism or other small-sized biologicals, is set up in culture chamber
There is at least one supporting plane for being used to place culture vessel, the main tank body is provided with gas outlet and sample tap, and sample tap
It is placed in main tank body top；
Gas supply mechanism, for filtrated air and/or default special gas to be passed through in culture chamber；
Escaping gas collector, the escaping gas collector can connect and biological for Real-time Collection with sample tap
It is metabolized the volatile molecules for producing.
As a further improvement on the present invention, several supporting planes are provided with culture chamber internal frame.
As a further improvement on the present invention, the escaping gas collector is set to stretch into micro- solid phase extraction of sample tap
Take post or micro- SPE pin.
Further improved as of the invention, the sample tap is set to multiple, and micro- solid-phase extraction column or micro- solid phase
Extraction pin is correspondingly arranged with sample tap.
As a further improvement on the present invention, the main tank body is provided with the housing of opening and is sealingly mounted at shell including top
Lid at body top end opening, the culture chamber is surrounded and is formed by housing and lid sealing, and the gas outlet and sample tap are all provided with
Put on lid.
Further improved as of the invention, the housing is set to heatable glass material, and is set on housing
There is the interlayer for being incubated.
As a further improvement on the present invention, the gas supply mechanism includes being used for filtrated air and/or presets special gas
Body pumps into the peristaltic pump in culture chamber.
Further improved as of the invention, outside air and/or default special gas are carried out after sending by peristaltic pump
The flow control of air inlet, and pumped into culture chamber by sterilizing filter.
Further improved as of the invention, threeway was provided with before sterilizing filter, the threeway has two
Entrance and one outlet, the outlet of the threeway are connected with the entrance of sterilizing filter, and one of entrance is sent for reception
Outside air, another entrance is used to receive the default special gas sent.
As a further improvement on the present invention, the gas supply mechanism includes being circumferentially positioned at culture chamber bottom and positioned at support
Air sparger below plane, the air sparger is used for the filtrated air that will be sent and/or the dispersion of default special gas
Out.
The real-time collecting method of the collection device of the volatile gas that biological metabolism is produced, comprises the following steps：
Sample is placed in main tank body；
Control condition of culture, specifically refers to：Main tank body control growth temperature is controlled, controls gas supply mechanism to control main tank body
Interior gas componant, including peristaltic pump flow control, the gas component ratio of regulation gas supply container；
After stabilization Preset Time, extract the gas at the top of main tank body or carried out in its top micro- solid-phase extraction column/pin of insertion
Extraction；
Extraction is tested and analyzed after terminating.
Based on above-mentioned technical proposal, the embodiment of the present invention can at least produce following technique effect：Integral structure layout is tight
Gather, reliable operation, main tank body is a kind of sterile culture device, the condition of culture of its tiny organism is controllable, including to growth temperature
Control, the composition of air in growing space is controlled, it is also possible to the default special gas composition of addition, and nothing in growing space
Bacterium controls, so as to carry out stimulation test to target organism growth；Sample tap is provided with main tank body top and close-connected wave
Hair property gas collector, can under the conditions of growth stimulation real time sample, and then realize being grown in microorganism or other small-sized biologicals
When, carry out the Real-time Collection of the volatile molecules of metabolism generation.
Brief description of the drawings
Specific embodiment of the invention is described in further detail below in conjunction with the accompanying drawings, wherein：
Fig. 1 is the structural representation of collection device in a preferred embodiment of the present invention.
In figure, 10, main tank body；11st, culture chamber；12nd, gas outlet；13rd, sample tap；14th, housing；15th, lid；16th, enter under
The mouth of a river；17th, upper water-out mouthful；20th, supporting plane；31st, sterilizing filter；32nd, threeway；33rd, air sparger.
Specific embodiment
The following is specific embodiment of the invention and with reference to accompanying drawing, technical scheme is further described,
But the present invention is not limited to these embodiments.
A kind of controllable collection device of present invention condition of culture of protection, can when microorganism or other small-sized biologicals grow,
Carry out the Real-time Collection of the volatile molecules of metabolism generation.
The technical scheme that the present invention is provided is illustrated in more detail with reference to Fig. 1.
As shown in figure 1, the collection device of the volatile gas of this biological metabolism generation includes：
Main tank body 10, is provided with the closed culture chamber 11 for cultivating microorganism or other small-sized biologicals, in culture chamber 11
Internal frame is provided with least one is used to place the supporting plane 20 of culture vessel, and the main tank body 10 is provided with gas outlet 12 and sampling
Mouth 13, and sample tap 13 is placed in the top of main tank body 10；
Gas supply mechanism, for filtrated air and/or default special gas to be passed through in culture chamber 11；
Escaping gas collector (not shown), the escaping gas collector can connect with sample tap 13 and be used in combination
In the volatile molecules that Real-time Collection biological metabolism is produced.
In the present invention, it is preferred to supporting plane 20 is arranged inside (culture chamber 11) bottom of main tank body 10 or the top position in bottom
Put, further preferred above-mentioned supporting plane is set to multiple, it is ensured that training reliability, the volatile molecules of generation (wave by gas
Stimulating food) reliably rise and be collected, gas supply mechanism, escaping gas collector are equipped with sealing knot with the contact portion of main tank body 10
Structure, to ensure sealing.
The collection device integral structure layout of the volatile gas that this biological metabolism is produced is compact, reliable operation, main tank body
10 (culture chambers 11) are a kind of sterile culture devices, and the condition of culture of its tiny organism is controllable, including to growth temperature control, right
Composition of air in growing space is controlled, it is also possible to the default special gas composition of addition, and aseptic controlling in growing space,
So as to carry out stimulation test to target organism growth；Sample tap 13 and close-connected volatility are provided with the top of main tank body 10
Gas collector, can under the conditions of growth stimulation real time sample, and then realize, when microorganism or other small-sized biologicals grow, entering
The Real-time Collection of the volatile molecules that row metabolism is produced.
Preferably, escaping gas collector is set to stretch into micro- solid-phase extraction column of sample tap 13 or micro- SPE
Pin.This main tank body 10 uses headspace enrichment mode, and main tank is entered from sample tap 13 with micro- solid-phase extraction column or micro- SPE pin
In body 10, the volatile gas componant for collecting tiny organism (bacterium colony) generation conveniently carries out follow-up analyzing and identifying.
It is further preferred that sample tap 13 is set to multiple, and micro- solid-phase extraction column or micro- SPE pin and sample tap
13 are correspondingly arranged, and such topology layout ensure that the reliability and accuracy of sampling work.Certainly, other are extracted using top
Gas or top insert the scheme of micro- solid-phase extraction column/pin carry out the mode of gas collection be also it is feasible, herein no longer
Repeat.
In the present invention, main tank body 10 is provided with the housing 14 of opening and is sealingly mounted at the top end opening of housing 14 including top
The lid 15 at place, the culture chamber 11 is surrounded and is formed by housing 14 and the sealing of lid 15, and the gas outlet 12 and sample tap 13 are equal
It is arranged on lid 15.
Such convenient sealing space placed culture vessel etc., also ensure culture chamber 11 of the layout of main tank body 10, it is above-mentioned micro-
Solid-phase extraction column or micro- SPE pin pass vertically through lid 15 and stretch into culture chamber 11.It is also excellent to ensure the reliability of work
Select lid 15 for stainless steel material, it, by rubber seal, increased seal with the contact surface of housing 14.
Further, to ensure the reliability of work and the stability of tiny organism culture, housing 14 is set to heat
Glass material, and be provided with housing 14 for be incubated interlayer.Further, interlayer is controlled for leading to the water of uniform temperature
Temperature processed, interlayer has lower water inlet 16 and upper water-out mouthful 17, for connecting external water bath device.
Preferably, gas supply mechanism includes outer in culture chamber 11 for filtrated air and/or default special gas to be pumped into
Put peristaltic pump (not shown).Using external peristaltic pump as the power source for conveying gas, the supply of device is by peristaltic pump control
Make and pump into culture chamber 11 so that air fluid flowing is gentle, lasting, is alternately squeezed by the elastic delivery hose to pump
Press and discharge to pump gas, negative pressure is formed in pipe, air fluid flows therewith.
Further, in the present invention, outside air and/or default special gas carry out air inlet after sending by peristaltic pump
Flow is controlled, and is pumped into culture chamber 11 by sterilizing filter 31.
Pneumatic filter (i.e. above-mentioned sterilizing filter 31) is provided with this collection device, specifically, what the external world sent
Air, default special gas or the mixed gas fluid of the two, first pass through sterilizing filter 31 and filter, the filtrated air of generation
And/or default special gas fluid is pumped into the culture space of main tank body 10 again, it is ensured that the reliability of tiny organism incubation
And final product quality.
Further, steady to ensure supply, conveying stabilization was provided with threeway 32, preferably before sterilizing filter 31
Ground, the threeway 32 has two entrances and one outlet, and the outlet of the threeway 32 is connected with the entrance of sterilizing filter 31,
One of entrance is used to receive the outside air sent, and another entrance is used to receive the default special gas sent, three
Logical 32 each porch is preferably provided with switch valve, and preferably unidirectional valve, is single in order to control the conveying of gaseous fluid
Air, single other gases or mixed gas fluid.
Used as a kind of preferred or optional implementation method, gas supply mechanism includes being circumferentially positioned at the bottom of culture chamber 11 and being located at
The air sparger 33 of the lower section of supporting plane 20, the air sparger 33 is used for the filtrated air that will be sent and/or default spy
Different gas disperses out.Preferably, air sparger 33 has multiple equally distributed stomatas, can be used for the aseptic sky that will be sent
Gas and/or default special gas disperse out.
Main tank body 10 is preferably arranged to cylinder-like structure, and then it is loop configuration that above-mentioned air sparger 33 is correspondingly arranged,
Its gaseous fluid for being used to diffuse into culture space, makes it be evenly distributed.
Air sparger 33 in this case is preferably circulating line structure, and by the pipeline through lid 15 and aseptic mistake
Filter 31 is connected, and the front of sterilizing filter 31 is provided with threeway 32.Further, circular air sparger 33 is by stainless
Steel material is made, and above has the equally distributed stomata for being preferably 1-3mm diameters.
In the present invention, the operation principle of the collection device of the volatile gas that the biological metabolism is produced is as follows：Main tank body
10 housing 14 is glass material, by laminated heat-preserving；Lid 15 is stainless steel material, close by rubber blanket with the contact surface of housing 14
Envelope；There is planar support near bottom position in main tank body 10, multiple can be placed thereon and is uncapped (preferably single to 20)
Culture dish or other holders；It is supplied by peristaltic pump control device, and tank body space is pumped into by sterilizing filter 31；Nothing
Bacterium gas disperses out by the air sparger 33 of bottommost, flows through biologic strut flat board (i.e. above-mentioned supporting plane 20), most
The gas outlet 12 from lid 15 is flowed out afterwards；In the present case, by the selection of pump head rotating speed and pipe diameter, flow rate set scope
Can from 0.001 inner volume/minute to 20 inner volumes/minute.
Additionally, the device can both be passed through aseptic gas, and special gas composition, side are added at the external import of threeway 32
Just supply control is carried out；The convenient use headspace enrichment method of main tank body 10, with micro- solid-phase extraction column or micro- SPE pin from taking
Sample mouthful 13 enters in main tank body 10, is used to collect the volatile gas componant that microorganism or other tiny organisms are produced, convenient
Carry out follow-up analyzing and identifying.
The present invention also protects a kind of real-time collecting method of the collection device of the volatile gas of biological metabolism generation, its bag
Include following steps：
Place sample interior in main tank body 10 (closed container)；
Control condition of culture, specifically refers to：Control main tank body 10 to control growth temperature, control gas supply mechanism to control main tank
Gas componant in body 10, including peristaltic pump flow control, the gas component ratio of regulation gas supply container；
After stabilization Preset Time, extract the gas at the top of main tank body 10 or enter in its top micro- solid-phase extraction column/pin of insertion
Row extraction；
Extraction is tested and analyzed after terminating.
In this course, organic solvent is not required to, simple to operate, method is convenient；And condition of culture is controllable, the method energy
When microorganism or other small-sized biologicals grow, the Real-time Collection of the volatile molecules of metabolism generation is carried out, especially microorganism
The volatile small molecule secreted in growth course under controlled condition.The collection device in the present invention can be used for a certain amount of waving
Hair property molecule is for microorganism or the influence of the growth of other tiny organisms, physiology and metabolism.
In the present invention, the device is also provided and a kind of can study shadow of a certain amount of gas concentration lwevel for yeast growth
Ring, specific embodiment is as follows：
The controllable culture tank body of gas componant (ACI tank bodies, as shown in accompanying drawing 1) inner volume (preferably 2000mL)；By ACI tanks
Body, together with the subsidiary air inlet sebific duct for binding up with gauze and air cleaner, goes out in steam (preferable temperature is 121 DEG C) jointly
Bacterium Preset Time (preferably 20min)；Solid medium that is another to prepare sterile petri dish (preferably 10cm), pouring into after sterilizing,
And it is inoculated with the microorganism saccharomyces cerevisiae to be tested；10 vaccinated above-mentioned culture dishes are put into superclean bench, are cultivated
The upper lid of ware is discarded；The connection threeway 32 of sterilizing filter 31 of ACI tank body air inlets is managed, and an entrance point of the pipe of threeway 32 can be with
One carbon dioxide steel cylinder is coupled by sebific duct, another import termination sebific duct, sebific duct openend is coated with sterile gauze, exposure
In atmosphere；Connection threeway 32 pipe controls induction air flow ratio with the sebific duct of carbon dioxide steel cylinder with peristaltic pump, is set as 1mL/min；
Connection threeway 32 pipe controls induction air flow ratio with the sebific duct of air with peristaltic pump, is set as 19mL/min；Ventilate herein control condition
Under, the entrance gas flow rate of ACI tank bodies is 20mL/min, and the ratio of carbon dioxide and air can be controlled in 1:19, it is hourly
Air replacement rate is 0.6 inner volume；Water inlet 16 and upper water-out mouthful 17 couple other circulations under ACI tank body thermal insulation clamp jacket layers
Water bath device；Set 30 DEG C of the water temperature of circulator bath；Extraction column/pin socket is left in the upper lid 15 of ACI tank bodies；In culture
In the period of five days, extraction column/pin can be inserted into ACI tank interiors space according to set time, for example every 12h, be made
The seizure of volatile gas molecule in brewer yeast growth process.
In the present embodiment, above-mentioned measure word is preferred value, does not limit the actual implementation method of the present embodiment.
The device is also provided and a kind of can study reality of a certain amount of gas concentration lwevel for influence that Trichoderma viride grows
Under applying for example：
ACI tank bodies (with above-mentioned, seeing the main tank body of accompanying drawing 1) inner volume 2000mL.By ACI tank bodies, yarn is used together with subsidiary
Air inlet sebific duct and air cleaner that cloth is wrapped, jointly in 121 DEG C of steam sterilizing 20min.The another sterile culture for preparing 10cm
Ware, the solid medium poured into after sterilizing, and it is inoculated with the micro organism green trichoderma to be tested.8 are put into superclean bench
Only vaccinated above-mentioned culture dish, the upper lid of culture dish is discarded.The connection threeway 32 of sterilizing filter 31 of ACI tank body air inlets
Pipe a, entrance point of the pipe of threeway 32 can couple a carbon dioxide steel cylinder, another import termination sebific duct, glue by sebific duct
Tube opening end is coated with sterile gauze, and exposure is in atmosphere.The sebific duct peristaltic pump of connection threeway 32 pipe and carbon dioxide steel cylinder
Control induction air flow ratio, is set as 4mL/min.Connection threeway 32 pipe controls induction air flow ratio, setting with the sebific duct of air with peristaltic pump
It is 44mL/min.Herein under ventilation control condition, the entrance gas flow rate of ACI tank bodies is 48mL/min, carbon dioxide and air
Ratio can be controlled in 1:11, air replacement rate hourly is 2.4 inner volumes.Intake under ACI tank body thermal insulation clamp jacket layers
Mouth 16 and upper water-out mouthful 17 couple other circulator bath devices.Set 30 DEG C of the water temperature of circulator bath.The upper lid of ACI tank bodies
Extraction column/pin socket is left on 15.Cultivate seven days in the period of in, extraction column/pin can according to set time, for example every
12h, is inserted into ACI tank interiors space, carries out the seizure of volatile gas molecule in Trichoderma viride growth course.
In the present embodiment, above-mentioned measure word is preferred value, does not limit the actual implementation method of the present embodiment.
The device also provide it is a kind of can study a certain amount of ethyl acetate and be produced for Trichoderma viride have the fibre of economic worth
The embodiment of the influence of the plain enzyme of dimension is as follows：
ACI tank bodies (with above-mentioned) inner volume 2000mL.By ACI tank bodies, together with the subsidiary air inlet chewing-gum for binding up with gauze
Pipe and air cleaner, jointly in 121 DEG C of steam sterilizing 20min.The another sterile petri dish for preparing 10cm, pours into consolidating after sterilizing
Body culture medium, and it is inoculated with the micro organism green trichoderma to be tested.8 vaccinated above-mentioned cultures are put into superclean bench
Ware, the upper lid of culture dish is discarded.The connection threeway 32 of sterilizing filter 31 of ACI tank body air inlets is managed, and of the pipe of threeway 32 enters
Mouth end can couple a vial for filling 500mL organic liquid ethyl acetate by sebific duct.The vial volume is
1000mL, bottleneck is sealed with lid, and two stainless steel hollow tubes are stretched out on lid.One of hollow pipe port of stainless steel is with yarn
Cloth is coated, and exposure is in atmosphere；Another stainless steel hollow tube couples above-mentioned threeway 32 and manages with silicone tube.Connection threeway 32 pipe with
The sebific duct for filling the vial of 500mL organic liquid ethyl acetate controls induction air flow ratio with peristaltic pump, is set as 0.2mL/min.
Connection threeway 32 pipe controls induction air flow ratio with the sebific duct of air with peristaltic pump, is set as 39.8mL/min.Ventilate herein control strip
Under part, the entrance gas flow rate of ACI tank bodies is 40mL/min, the air of ethyl acetate saturation and the flow velocity of another air pipe line
Ratio can be controlled in 1:199, air replacement rate hourly is 1.2 inner volumes.Water inlet under ACI tank body thermal insulation clamp jacket layers
16 and upper water-out mouthfuls 17 couple other circulator bath devices.28 DEG C of water temperature for setting circulator bath carries out culture microorganism, can enter
Influence data record of the subsequent volatile acetic acid ethyl ester of row to cellulase produced by Trichoderma viride.
In the present embodiment, above-mentioned measure word is preferred value, does not limit the actual implementation method of the present embodiment.
Specific embodiment described herein is only to the spiritual explanation for example of the present invention.Technology neck belonging to of the invention
The technical staff in domain can be made various modifications or supplement to described specific embodiment or be replaced using similar mode
Generation, but without departing from spirit of the invention or surmount scope defined in appended claims.
Claims (10)
1. biological metabolism produce volatile gas collection device, it is characterised in that：Including：
Main tank body, is provided with the closed culture chamber for cultivating microorganism or other small-sized biologicals, culture chamber internal frame be provided with to
Few one supporting plane for being used to place culture vessel, the main tank body is provided with gas outlet and sample tap, and sample tap is placed in
Main tank body top；
Gas supply mechanism, for filtrated air and/or default special gas to be passed through in culture chamber；
Escaping gas collector, the escaping gas collector can connect and for Real-time Collection biological metabolism with sample tap
The volatile molecules of generation.
2. the collection device of the volatile gas that biological metabolism according to claim 1 is produced, it is characterised in that：In culture
Chamber internal frame is provided with several supporting planes.
3. the collection device of the volatile gas that biological metabolism according to claim 1 is produced, it is characterised in that：The sky
Gas collector is set to stretch into micro- solid-phase extraction column or micro- SPE pin of sample tap.
4. the collection device of the volatile gas that biological metabolism according to claim 1 is produced, it is characterised in that：The master
Tank body is provided with the housing of opening and the lid being sealingly mounted at housing tip opening including top, the culture chamber by housing and
Lid sealing is surrounded to be formed, and the gas outlet and sample tap are arranged on lid.
5. the collection device of the volatile gas that biological metabolism according to claim 4 is produced, it is characterised in that：The shell
Body is set to heatable glass material, and the interlayer for being incubated is provided with housing.
6. the collection device of the volatile gas that biological metabolism according to claim 1 is produced, it is characterised in that：The confession
Mechanism of qi structure is included for filtrated air and/or default special gas to be pumped into the peristaltic pump in culture chamber.
7. the collection device of the volatile gas that biological metabolism according to claim 6 is produced, it is characterised in that：It is extraneous empty
The flow that gas and/or default special gas carry out air inlet after sending by peristaltic pump is controlled, and pumps into culture by sterilizing filter
In chamber.
8. the collection device of the volatile gas that biological metabolism according to claim 7 is produced, it is characterised in that：Aseptic
Threeway is provided with before filter, the threeway has two entrances and one outlet, outlet and the aseptic filtration of the threeway
The entrance of device is connected, and one of entrance is used to receive the outside air sent, and another entrance is for presetting that reception is sent
Special gas.
9. the collection device of the volatile gas that biological metabolism according to claim 1 is produced, it is characterised in that：The confession
Mechanism of qi structure includes being circumferentially positioned at culture chamber bottom and the air sparger below supporting plane, and the air sparger is used
Disperse out in the filtrated air that will be sent and/or default special gas.
10. the Real-time Collection side of the collection device of the volatile gas that biological metabolism described in any one of claim 1 to 9 is produced
Method, it is characterised in that：Comprise the following steps：
Sample is placed in main tank body；
Control condition of culture, specifically refers to：Main tank body control growth temperature is controlled, controls gas supply mechanism to control in main tank body
Gas componant, including peristaltic pump flow control, the gas component ratio of regulation gas supply container；
After stabilization Preset Time, extract the gas at the top of main tank body or extracted in its top micro- solid-phase extraction column/pin of insertion；
Extraction is tested and analyzed after terminating.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611218248.0A CN106701547A (en) | 2016-12-26 | 2016-12-26 | Device for collecting gas volatile matters produced by biological metabolism and real-time collection method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
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| CN111286442A (en) * | 2018-12-07 | 2020-06-16 | 国投生物科技投资有限公司 | Device for culturing microorganisms to obtain volatile products, method and application thereof |
| CN112841069A (en) * | 2021-02-26 | 2021-05-28 | 公安部南昌警犬基地 | Training method and auxiliary device for identifying dogs for lung cancer based on exhaled air of people |
| US20220002647A1 (en) * | 2020-07-02 | 2022-01-06 | Lg Chem, Ltd. | Gas Collection Device |
| CN113906925A (en) * | 2021-10-29 | 2022-01-11 | 福建农林大学 | Non-damage device for collecting volatile matters in root system |
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