CN205662523U - Plant cell cultivates teaching and uses plain type bioreactor - Google Patents
Plant cell cultivates teaching and uses plain type bioreactor Download PDFInfo
- Publication number
- CN205662523U CN205662523U CN201620534775.1U CN201620534775U CN205662523U CN 205662523 U CN205662523 U CN 205662523U CN 201620534775 U CN201620534775 U CN 201620534775U CN 205662523 U CN205662523 U CN 205662523U
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- China
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- air
- bottle
- culture
- plant cell
- blake bottle
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses a plant cell cultivates teaching and uses plain type bioreactor relates to plant cell engineering technical field. Including the blake bottle, the blake bottle top is equipped with the bottle lid air -breather and includes air outlet duct, and upper portion in bottle lid to the blake bottle is passed to air outlet duct one end, and the other end lies in the blake bottle outside and connects gradually " Y " venturi tube, air filter, the blake bottle shoulder is equipped with air inlet unit, passes the air inlet plug including air induction conduit one end and connects glass pipe and gas tip arrival blake bottle bottom, and the other end lies in the blake bottle outside and connects gradually air filter, air slide, air pump, the blake bottle shoulder also has another sampling device, this for the sample connection plug seal, produce (or shao) with the cell during sample under aseptic condition and take out the cell. The utility model discloses small, with low costs, the controllable speed of ventilating of reactor, easy operation easily installs and dismantles, is fit for the experiment course operation simultaneously of more student.
Description
Technical field
This utility model relates to a kind of biological reaction apparatus, and culture plant cell bioreactor is used in specifically a kind of teaching
Application apparatus, belongs to plant cell engineering field.
Background technology
Plant tissue culture technique brings revolutionary change to plant propagation, and the most a lot of plants can be carried out
Commercialization micropropagation.Bioreactor utilizes fluid medium, and under conditions of being totally submerged, ventilation is greatly improved life by force
The growth rate of thing amount, and the automatization of condition of culture control and operation can be realized, thus saved labour force, reduced
Production cost, shorten incubation time.Along with the bioreactor extensive application in plant, higher education is in plant cell work
Journey laboratory introduces bioreactor large-scale culture cell, but costly and build is bigger because of bioreactor, it is impossible to enter
Entering aseptic operating platform, student can only replace by viewing lecture experiment or by conical flask, thus can not allow student's profound understanding
Bioreactor culture cell mechanism, it is impossible to the skilled production procedure grasping bioreactor and operating technology, therefore, designs one
Kind of small size, low cost, be suitable for more student carry out simultaneously experimental courses and personally operate, be applicable to culture plant cell
Bioreactor, is to be presently required to solve the technical problem that.
Summary of the invention
The technical problems to be solved in the utility model is to provide the bioreactor of a kind of plain type teaching, and this biology is anti-
Answer body to amass little, low cost, ventable, it is easy to installation and removal, be suitable for more student and carry out experimental courses simultaneously.
For solving above-mentioned technical problem, technical solution adopted in the utility model is the biology of a kind of plain type teaching
Reactor, including culture apparatus, exhaust apparatus and inoculation sampler.
Culture apparatus is the culture bottle converted with 500ml reagent bottle, and culture bottle right and left shoulders respectively has a mouth: one
Individual air inlet 11, an inoculation and sample tap 10, a gas outlet 12 is arranged at culture bottle top.Cultivate a mouth glue on shoulder
Plug 13 seals, as inoculation and sample tap；Another mouthful plug with glass tubing seals, as air inlet；Culture bottle top
First cover with the plug of band steel pipe, then cover, as gas outlet with the get beat up former lid in hole of reagent bottle.
Exhaust apparatus is divided into air intake installation and air-out apparatus, air intake installation be with air pump 2 be sequentially connected with rubber hose for medical 7,
Air regulating piston 3, filter 4, culture bottle air inlet 11, the air inlet in culture bottle is connected with rubber hose for medical 7 and gas spray
6, the ceramic Fructus Amomi in the i.e. aquarium of shower nozzle；Air-out apparatus is to be sequentially connected with rubber hose for medical 7, Y-shaped in the gas outlet of culture bottle
Glass tubing 9, the head of Y-shaped glass tubing is connected with rubber hose for medical 7 and filter 4 respectively.
Inoculation sampler is at the sample tap cultivated on shoulder, seals with plug 13, is not connected to any material, and inoculation takes
During sample, aseptically opening plug, medication spoon accesses cell, or goes out the cell or cultivation needed with pipettor, medicine bale-out
Liquid.
Air filter all uses diameter 5cm, containing the filter of aperture 0.22 micron membrane filter.
Accompanying drawing explanation
Fig. 1 bioreactor assembles figure
Detailed description of the invention
Embodiment 1:
A kind of culture plant cell teaching uses plain type bioreactor, experimental selection bubble to agitate ventilating mode to train
Supporting, using the reagent bottle 1 after 500mL repacking is bioreactor.The gas tip i.e. ceramic in aquarium in bubbling training method
Fructus Amomi, bubbling plenum system is made up of assemblies such as air pump, gas filter, gas inlet and gas outlets.
Breather includes air pump 2, air slide 3, air filter 4, air induction conduit 5, and gas tip 6, between each device
It is connected with medical latex conduit 7.Gas is pumped into by air pump, by conduit through air slide, regulates the size of induction air flow ratio, then
Remove antibacterial through filter, enter bottom bioreactor along conduit, gas tip discharge and produce a large amount of bubbles, make suspension thin
Born of the same parents do not stop to move about.
Air-out apparatus includes outtake tube 8, " Y " font pipe 9, filter 4, is connected with medical latex conduit 7 between each device.
Gas in bioreactor is by outtake tube, through " Y " font pipe, filter discharge.
Filler and sampler 10, aseptically take off plug, can carry out filler or sampling.
This bioreactor application example 2:
1, culture plant cell: Cortex Fraxini mandshuricae callus is inoculated into WPM+0.15mg/L2,4-D+0.1mg/LBA+20g
Suspension culture in the fluid medium of sucrose.
2, the application of bioreactor: add the WPM+0.15mg/L2,4-D+0.1mg/LBA+20g of 300mL in reactor
The fluid medium of sucrose, aseptically inoculation 20g Cortex Fraxini mandshuricae suspension cell, cultivate 5d, and 10d, 15d, 20d detect respectively
Indices.
3, the change of cellular biomass: cell is placed in 60 degree of baking ovens overnight, claims its dry weight in second day.Doing of cell
Weight is gradually increased along with the increase of incubation time, and increase weight 2.78g when 20d.
Claims (4)
1. a plain type bioreactor is used in culture plant cell teaching, is provided with exhaust apparatus including culture bottle 1, top, training
Support bottle 1 shoulder and be provided with inoculation sampler and air intake installation；
Exhaust apparatus includes outtake tube, and outtake tube one end is positioned at culture bottle through bottle cap to culture bottle middle and upper part, the other end
Outside is also sequentially connected with Y-shaped pipe, air filter；Culture bottle shoulder is provided with air intake installation, including air induction conduit one end pass into
QI KOU plug connects glass tubing and gas tip arrives bottom culture bottle, and the other end is positioned at outside culture bottle and is sequentially connected with air filter
Device, air slide, air pump；Culture bottle shoulder also has another sampler, and this sample tap plug seals, in nothing during sampling
Produce (or spoon) with cell under the conditions of bacterium and take out cell.
A kind of culture plant cell teaching plain type bioreactor the most according to claim 1, it is characterised in that row
Device of air exit connects Y-shaped pipe.
A kind of culture plant cell teaching plain type bioreactor the most according to claim 1, it is characterised in that training
Support bottle glass reagent bottle to reequip.
A kind of culture plant cell teaching plain type bioreactor the most according to claim 1, it is characterised in that institute
Mouth is had all to have rubber stopper seal.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620534775.1U CN205662523U (en) | 2016-06-06 | 2016-06-06 | Plant cell cultivates teaching and uses plain type bioreactor |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620534775.1U CN205662523U (en) | 2016-06-06 | 2016-06-06 | Plant cell cultivates teaching and uses plain type bioreactor |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN205662523U true CN205662523U (en) | 2016-10-26 |
Family
ID=57158716
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201620534775.1U Expired - Fee Related CN205662523U (en) | 2016-06-06 | 2016-06-06 | Plant cell cultivates teaching and uses plain type bioreactor |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN205662523U (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106434314A (en) * | 2016-11-02 | 2017-02-22 | 广东石油化工学院 | Culture bottle air change device |
| CN106701547A (en) * | 2016-12-26 | 2017-05-24 | 浙江海洋大学 | Device for collecting gas volatile matters produced by biological metabolism and real-time collection method thereof |
| CN108285862A (en) * | 2018-04-18 | 2018-07-17 | 中国农业科学院农业资源与农业区划研究所 | Microbial ferment device |
| CN111366423A (en) * | 2020-04-22 | 2020-07-03 | 济南大学 | Device for collecting soil breathing gas and gas collecting method |
-
2016
- 2016-06-06 CN CN201620534775.1U patent/CN205662523U/en not_active Expired - Fee Related
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106434314A (en) * | 2016-11-02 | 2017-02-22 | 广东石油化工学院 | Culture bottle air change device |
| CN106701547A (en) * | 2016-12-26 | 2017-05-24 | 浙江海洋大学 | Device for collecting gas volatile matters produced by biological metabolism and real-time collection method thereof |
| CN108285862A (en) * | 2018-04-18 | 2018-07-17 | 中国农业科学院农业资源与农业区划研究所 | Microbial ferment device |
| CN111366423A (en) * | 2020-04-22 | 2020-07-03 | 济南大学 | Device for collecting soil breathing gas and gas collecting method |
| CN111366423B (en) * | 2020-04-22 | 2024-05-14 | 济南大学 | Device for collecting soil respiratory gas and gas collection method |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20161026 Termination date: 20170606 |
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| CF01 | Termination of patent right due to non-payment of annual fee |