CN111366423A - Device for collecting soil breathing gas and gas collecting method - Google Patents
Device for collecting soil breathing gas and gas collecting method Download PDFInfo
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- CN111366423A CN111366423A CN202010322317.2A CN202010322317A CN111366423A CN 111366423 A CN111366423 A CN 111366423A CN 202010322317 A CN202010322317 A CN 202010322317A CN 111366423 A CN111366423 A CN 111366423A
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- 239000002689 soil Substances 0.000 title claims abstract description 39
- 238000000034 method Methods 0.000 title claims abstract description 31
- 230000029058 respiratory gaseous exchange Effects 0.000 title claims abstract description 7
- 239000011521 glass Substances 0.000 claims abstract description 63
- 238000005070 sampling Methods 0.000 claims abstract description 48
- 238000007789 sealing Methods 0.000 claims abstract description 39
- 238000005259 measurement Methods 0.000 claims description 7
- 238000007599 discharging Methods 0.000 claims description 4
- 238000011010 flushing procedure Methods 0.000 claims description 4
- 239000003292 glue Substances 0.000 claims description 3
- 239000004519 grease Substances 0.000 claims description 3
- 229920001296 polysiloxane Polymers 0.000 claims description 3
- 239000000565 sealant Substances 0.000 claims description 3
- 239000002699 waste material Substances 0.000 abstract description 5
- 239000003513 alkali Substances 0.000 description 9
- 238000009991 scouring Methods 0.000 description 6
- 238000004158 soil respiration Methods 0.000 description 6
- 238000010521 absorption reaction Methods 0.000 description 5
- 230000000694 effects Effects 0.000 description 4
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- 230000003068 static effect Effects 0.000 description 3
- 230000004071 biological effect Effects 0.000 description 2
- 230000001066 destructive effect Effects 0.000 description 2
- 238000004817 gas chromatography Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 229930182555 Penicillin Natural products 0.000 description 1
- JGSARLDLIJGVTE-MBNYWOFBSA-N Penicillin G Chemical compound N([C@H]1[C@H]2SC([C@@H](N2C1=O)C(O)=O)(C)C)C(=O)CC1=CC=CC=C1 JGSARLDLIJGVTE-MBNYWOFBSA-N 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 238000007792 addition Methods 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000000090 biomarker Substances 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- BVKZGUZCCUSVTD-UHFFFAOYSA-N carbonic acid Chemical compound OC(O)=O BVKZGUZCCUSVTD-UHFFFAOYSA-N 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 230000035558 fertility Effects 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000006386 neutralization reaction Methods 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 230000000149 penetrating effect Effects 0.000 description 1
- 229940049954 penicillin Drugs 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 238000004448 titration Methods 0.000 description 1
Images
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/02—Devices for withdrawing samples
- G01N1/22—Devices for withdrawing samples in the gaseous state
- G01N1/2294—Sampling soil gases or the like
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Soil Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The invention relates to a device for collecting soil breathing gas, which comprises a glass bottle, a bottle cap arranged on the glass bottle and used for sealing the glass bottle, and a gas conduit which penetrates through the bottle cap and is communicated with the inside and the outside of the glass bottle; placing a soil sample to be detected in a glass bottle; the gas guide pipe comprises a sampling guide pipe, a gas inlet guide pipe and a gas outlet guide pipe, wherein the gas inlet end of the gas inlet guide pipe, the gas outlet end of the gas outlet guide pipe and the gas outlet end of the sampling guide pipe are positioned outside the glass bottle, the gas outlet end of the gas inlet guide pipe extends into the bottom of the glass bottle, and the gas inlet end of the gas outlet guide pipe and the gas inlet end of the sampling guide pipe are positioned at the upper part of the glass bottle; the air inlet end of the air inlet guide pipe, the air outlet end of the air outlet guide pipe and the air outlet end of the sampling guide pipe are respectively connected with a sealing port, and each sealing port is detachably connected with an end cap seal; a rubber stopper was installed in the sealing port on the sampling tube. The whole process can not cause any consumption waste, is efficient and saves the cost.
Description
Technical Field
The invention relates to the technical field of soil measurement, in particular to a device for collecting soil breathing gas and a gas collecting method.
Background
Soil respiration is an important biological indicator for characterizing soil quality and fertility, and reflects the biological activity of soil and the metabolic strength of soil substances. In the ecological succession process, the change of the vegetation affects the physical, chemical and biological properties of the soil by absorbing nutrients, returning organic matters and the like, and the soil respiration changes along with the change, thereby indicating the process and the direction of the succession of the ecological system. Furthermore, from a microclimate point of view, the soil releases CO2Changes microclimate conditions near the ground and provides richer carbon sources for the lower canopy of the plant.
The soil respiration measuring method at the present stage mainly comprises a static alkali liquor absorption method and a closed air chamber method. The static alkali solution absorption method uses alkali solution (NaOH or KOH solution), and researchers also use solid alkali particles, and the principle is the same, namely the alkali absorbs CO2Carbonate is formed, the residual alkali amount is calculated by a gravimetric method or a neutralization titration method, and CO discharged by the soil within a certain time is calculated according to a formula2Total amount of the components. The closed gas cell method comprises gas chromatography and infrared CO2Two analysis methods are that the experimental soil is put in a closed container, after a period of time is stable, the container is covered, a needle-shaped connector is used to extract gas sample into the vacuum container at a certain time interval, and a gas chromatograph or an infrared analyzer is used to measure CO in the gas sample2The concentration of (A) is calculated to obtain CO2The rate of discharge.
The static alkali liquor absorption method has the limitation that the measurement precision is not ideal, the measurement result is higher than the true value under the condition that the soil respiration rate is low, and the measurement result is lower than the true value under the condition that the soil respiration rate is high. The limitation of the closed air chamber method is that the sampling interval cannot be too short, and the same volume of air needs to be supplemented; meanwhile, the commonly used sealed culture devices are penicillin bottles and sealed Meisen cans, destructive sampling is mostly adopted in the sampling process, the rubber plug or the sealing film needs to be installed again for secondary sealing, the operation process is complex, and the rubber plug and the sealing film cannot be reused after the sampling is destroyed, so that resource waste is caused.
Disclosure of Invention
Aiming at the defects of the prior art, the invention provides the device for collecting the soil breathing gas and the gas collecting method, the whole process does not cause any consumption and waste, and the device is efficient and saves the cost.
In a first aspect of the invention, a device for collecting soil breathing gas is provided, which comprises a glass bottle, a bottle cap arranged on the glass bottle and used for sealing the glass bottle, and a gas conduit penetrating through the bottle cap and communicating the inside and the outside of the glass bottle; placing a soil sample to be detected in a glass bottle; the gas guide pipe comprises a sampling guide pipe, a gas inlet guide pipe and a gas outlet guide pipe, wherein the gas inlet end of the gas inlet guide pipe, the gas outlet end of the gas outlet guide pipe and the gas outlet end of the sampling guide pipe are positioned outside the glass bottle, the gas outlet end of the gas inlet guide pipe extends into the bottom of the glass bottle, and the gas inlet end of the gas outlet guide pipe and the gas inlet end of the sampling guide pipe are positioned at the upper part of the glass bottle; the air inlet end of the air inlet guide pipe, the air outlet end of the air outlet guide pipe and the air outlet end of the sampling guide pipe are respectively connected with a sealing port, and each sealing port is detachably connected with an end cap seal; a rubber stopper was installed in the sealing port on the sampling tube.
Preferably, the glass bottle is in threaded connection with the bottle cap.
Preferably, vacuum silicone grease is coated on the joint of the glass bottle and the bottle cap.
Preferably, the soil sample to be tested is contained in a beaker, which is placed in a glass bottle.
Preferably, the joint of the gas conduit and the bottle cap is sealed by a sealant. The sealing effect in the glass bottle is ensured.
Preferably, the end cap seal and sealing port are threaded. The sealing port is matched with the seal of the end cap to realize sealing.
Preferably, a sealing glue is coated at the joint of the rubber plug and the sealing port. Can further guarantee the sealed effect between rubber buffer and the sealed port, can prevent through the rubber buffer that sample in-process gas from revealing.
Preferably, the sampling conduit, the gas inlet conduit and the gas outlet conduit are all rubber tubes.
Preferably, the sampling tube, the air inlet tube and the air outlet tube located outside the glass bottle are respectively clamped by using spring gas-stop clamps. The spring gas-stopping clamp and the end cap seal are used for preventing gas leakage and ensuring the tightness of the soil sample culture process.
In a second aspect of the invention, there is provided a method of collecting gas using the apparatus described above, comprising the steps of:
s01: culturing the soil in the glass bottle for a period of time, opening an end cap on the sampling conduit and sealing, and extracting gas at the top of the glass bottle through a rubber plug in a sealing port of the sampling conduit by using an airtight syringe;
s02: after sampling is finished, screwing the end cap seal of the sampling guide pipe, opening the end cap seals on the air inlet guide pipe and the air outlet guide pipe, introducing air through the air inlet guide pipe, discharging air from the air outlet guide pipe, flushing gas in the glass bottle, and screwing the end cap seals on the air inlet guide pipe and the air outlet guide pipe after gas flushing is finished;
s03: the soil is continued to be cultured, and the subsequent measurement is repeated by the operation of step S01 and step S02.
The invention has the beneficial effects that:
the invention is provided with the sampling guide pipe, the air inlet guide pipe and the air outlet guide pipe, which respectively play roles of sampling, injecting scouring gas and discharging scouring gas, and can be sealed only by mounting again after sampling and scouring are finished, the whole process does not cause any consumption waste, the efficiency is high, the cost is saved, and the invention is a closed air chamber method, and has higher data accuracy compared with an alkali liquor absorption method.
Drawings
FIG. 1 is a schematic structural view of the present invention;
FIG. 2 is a schematic view of the bottle cap and the gas conduit according to the present invention;
FIG. 3 is a schematic view of a seal port and end cap seal configuration of a sample tube of the present invention;
FIG. 4 is a schematic view of a sealing port and end cap seal configuration for either an inlet conduit or an outlet conduit according to the present invention;
FIG. 5 is a schematic structural view of the spring check clamp of the present invention.
Shown in the figure:
1. glass bottle, 2, bottle lid, 3, sampling pipe, 4, gas inlet pipe, 5, the pipe of giving vent to anger, 6, sealed port, 7, end cap seal, 8, rubber buffer, 9, soil sample, 10, spring gas-stopping presss from both sides.
Detailed Description
In order to clearly illustrate the technical features of the present solution, the present solution is explained below by way of specific embodiments.
As shown in fig. 1, the present invention comprises a glass bottle 1, a cap 2 mounted on the glass bottle 1 for sealing the glass bottle 1, and a gas conduit passing through the cap 2 to communicate the inside and outside of the glass bottle 1. The glass bottle 1 is in threaded connection with the bottle cap 2, and vacuum silicone grease is smeared at the connection position of the glass bottle 1 and the bottle cap 2 to ensure sealing. The soil sample 9 to be detected is placed in the glass bottle 1, the soil sample 9 to be detected is placed in a beaker, and the beaker is placed in the glass bottle 1. The joint of the gas conduit and the bottle cap 2 is sealed by sealant, so that the sealing effect inside the glass bottle 1 is ensured.
As shown in fig. 1 and 2, the gas conduit comprises a sampling conduit 3, an air inlet conduit 4 and an air outlet conduit 5, wherein the air inlet end of the air inlet conduit 4 is positioned outside the glass bottle 1, and the air outlet end of the air inlet conduit 4 extends into the bottle inner bottom of the glass bottle 1. The air inlet end of the air outlet guide pipe 5 is positioned at the upper part in the glass bottle 1, and the air outlet end of the air outlet guide pipe 5 is positioned outside the glass bottle 1. The air inlet end of the sampling pipe 3 is positioned at the upper part in the glass bottle 1, and the air outlet end of the sampling pipe 3 is positioned outside the glass bottle 1.
As shown in fig. 3 and 4, the air inlet end of the air inlet duct 4, the air outlet end of the air outlet duct 5 and the air outlet end of the sampling duct 3 are respectively connected with a sealing port 6, an end cap seal 7 is detachably connected to each sealing port 6, the end cap seal 7 is in threaded connection with the sealing port 6, and the sealing port 6 is matched with the end cap seal 7 to realize sealing.
Install rubber buffer 8 in the sealed port 6 on sample pipe 3, can prevent through rubber buffer 8 that the gas from revealing in the sample process. In order to further ensure the sealing effect between the rubber plug 8 and the sealing port 6, a sealing glue is coated at the joint of the rubber plug 8 and the sealing port 6.
The sampling guide pipe 3, the air inlet guide pipe 4 and the air outlet guide pipe 5 are all rubber pipes, and the sampling guide pipe 3, the air inlet guide pipe 4 and the air outlet guide pipe 5 which are positioned outside the glass bottle 1 are respectively clamped by a spring air stopping clamp 10. The spring gas-stopping clamp 10 and the end cap seal 7 both have the functions of preventing gas leakage and ensuring the tightness of the soil sample 9 in the culture process.
During the concrete use, will need the soil sample 9 that awaits measuring to place in the beaker in glass bottle 1, seal glass bottle 1 through bottle lid 2, press from both sides tight sampling guide pipe 3, inlet duct 4 and outlet duct 5 respectively through three spring gas stopping clamp 10, seal end cap 7 and connect on sealed port 6, further seal.
The method for collecting gas by using the device comprises the following steps: after the device installation is accomplished, cultivate soil sample 9 according to the required condition of experiment, the gaseous accumulation in airtight bottle of the production of soil respiration in glass bottle 1, after certain incubation time, take off spring on the sample tube 3 and end gas clamp 10, open the end cap on the sample tube 3 and seal 7, use the gas tightness syringe to extract 1 top gas of glass bottle through the rubber buffer 8 in the sealed port 6 of sample tube 3, later use gas chromatography or infrared CO2The analyzer performs measurement; after the sample is accomplished, the end cap of screwing sampling pipe 3 seals 7, press from both sides the spring gas stop clamp 10 on the tight sampling pipe 3, open the end cap on inlet pipe 4 and the pipe 5 of giving vent to anger and seal 7, take off the spring gas stop clamp 10 on inlet pipe 4 and the pipe 5 of giving vent to anger, admit air through inlet pipe 4, give vent to anger from the pipe 5 of giving vent to anger, wash out gas in the glass bottle 1, gas washes and screws the end cap on inlet pipe 4 and the pipe 5 of giving vent to anger and seals 7 after accomplishing, press from both sides the spring gas stop clamp 10 on inlet pipe 4 and the pipe 5 of giving vent to anger, later continue to cultivate.
Traditional airtight air chamber culture apparatus need adopt destructive sampling, namely the airtight syringe pierces airtight rubber buffer 8 or the gas at the sealed lid extraction device interior top at glass bottle 1 top promptly, need use the air pump to carry out gas in the device to erode when cultivateing once more, then uses new sealed rubber buffer 8 to carry out glass bottle 1 again and seals. Compared with the prior art, the device is provided with the sampling guide pipe 3, the gas inlet guide pipe 4 and the gas outlet guide pipe 5, which respectively play roles in sampling, injecting scouring gas and discharging scouring gas, and can be sealed only by being installed again after sampling and scouring are completed, so that the whole process can not cause any consumption waste, is efficient and saves cost, and the device is a closed gas chamber method and has higher data accuracy compared with an alkali liquor absorption method.
Of course, the above description is not limited to the above examples, and the undescribed technical features of the present invention can be implemented by or using the prior art, and will not be described herein again; the above embodiments and drawings are only for illustrating the technical solutions of the present invention and not for limiting the present invention, and the present invention has been described in detail with reference to the preferred embodiments, it should be understood by those skilled in the art that changes, modifications, additions or substitutions within the spirit and scope of the present invention may be made by those skilled in the art without departing from the spirit of the present invention, and shall also fall within the scope of the claims of the present invention.
Claims (10)
1. A device for collecting soil breathing gas, characterized in that: comprises a glass bottle (1), a bottle cap (2) which is arranged on the glass bottle (1) and is used for sealing the glass bottle (1), and a gas conduit which penetrates through the bottle cap (2) and is communicated with the inside and the outside of the glass bottle (1); placing a soil sample (9) to be detected in the glass bottle (1); the gas guide pipe comprises a sampling guide pipe (3), a gas inlet guide pipe (4) and a gas outlet guide pipe (5), the gas inlet end of the gas inlet guide pipe (4), the gas outlet end of the gas outlet guide pipe (5) and the gas outlet end of the sampling guide pipe (3) are all located outside the glass bottle (1), the gas outlet end of the gas inlet guide pipe (4) extends into the bottom of the glass bottle (1), and the gas inlet end of the gas outlet guide pipe (5) and the gas inlet end of the sampling guide pipe (3) are all located on the upper portion of the glass bottle (1); the air inlet end of the air inlet guide pipe (4), the air outlet end of the air outlet guide pipe (5) and the air outlet end of the sampling guide pipe (3) are respectively connected with a sealing port (6), and each sealing port (6) is detachably connected with an end cap seal (7); a rubber plug (8) is arranged in a sealing port (6) on the sampling conduit (3).
2. A device for collecting soil respiring gas as claimed in claim 1, wherein: the glass bottle (1) is in threaded connection with the bottle cap (2).
3. A device for collecting soil respiring gas as claimed in claim 2, wherein: vacuum silicone grease is coated at the joint of the glass bottle (1) and the bottle cap (2).
4. A device for collecting soil respiring gas as claimed in claim 1, wherein: the soil sample (9) to be detected is placed in a beaker, and the beaker is placed in a glass bottle (1).
5. A device for collecting soil respiring gas as claimed in claim 1, wherein: the joint of the gas conduit and the bottle cap (2) is sealed by sealant.
6. A device for collecting soil respiring gas as claimed in claim 1, wherein: the end cap seal (7) is in threaded connection with the seal port (6).
7. A device for collecting soil respiring gas as claimed in claim 1, wherein: and a sealing glue is coated at the joint of the rubber plug (8) and the sealing port (6).
8. A device for collecting soil respiring gas as claimed in claim 1, wherein: the sampling guide pipe (3), the air inlet guide pipe (4) and the air outlet guide pipe (5) are all rubber pipes.
9. A device for collecting soil respiring gas as claimed in claim 8, wherein: the sampling conduit (3), the air inlet conduit (4) and the air outlet conduit (5) which are positioned outside the glass bottle (1) are respectively clamped by a spring air stop clamp (10).
10. A method of collecting gas using the apparatus of any one of claims 1 to 9, comprising the steps of:
s01: after soil in the glass bottle (1) is cultured for a period of time, an end cap seal (7) on the sampling conduit (3) is opened, and gas at the top of the glass bottle (1) is extracted through a rubber plug (8) in a sealing port (6) of the sampling conduit (3) by using an airtight syringe;
s02: after sampling is finished, screwing an end cap seal (7) of the sampling guide pipe (3), opening the end cap seals (7) on the air inlet guide pipe (4) and the air outlet guide pipe (5), introducing air through the air inlet guide pipe (4), discharging air from the air outlet guide pipe (5), flushing gas in the glass bottle (1), and screwing the end cap seals (7) on the air inlet guide pipe (4) and the air outlet guide pipe (5) after gas flushing is finished;
s03: the soil is continued to be cultured, and the subsequent measurement is repeated by the operation of step S01 and step S02.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010322317.2A CN111366423B (en) | 2020-04-22 | 2020-04-22 | Device for collecting soil respiratory gas and gas collection method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010322317.2A CN111366423B (en) | 2020-04-22 | 2020-04-22 | Device for collecting soil respiratory gas and gas collection method |
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| Publication Number | Publication Date |
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| CN111366423A true CN111366423A (en) | 2020-07-03 |
| CN111366423B CN111366423B (en) | 2024-05-14 |
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| CN202010322317.2A Active CN111366423B (en) | 2020-04-22 | 2020-04-22 | Device for collecting soil respiratory gas and gas collection method |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114166584A (en) * | 2021-12-06 | 2022-03-11 | 内蒙古自治区农牧业科学院 | Soil breathing gas collection device |
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| CN202322838U (en) * | 2011-10-30 | 2012-07-11 | 段为钢 | Microbial fermentation device |
| CN202916136U (en) * | 2012-10-24 | 2013-05-01 | 中国石油化工股份有限公司 | Airtight sampling device |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN114166584A (en) * | 2021-12-06 | 2022-03-11 | 内蒙古自治区农牧业科学院 | Soil breathing gas collection device |
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| CN111366423B (en) | 2024-05-14 |
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