CN106467892A - A kind of automatic cytological amplification system - Google Patents
A kind of automatic cytological amplification system Download PDFInfo
- Publication number
- CN106467892A CN106467892A CN201610800908.XA CN201610800908A CN106467892A CN 106467892 A CN106467892 A CN 106467892A CN 201610800908 A CN201610800908 A CN 201610800908A CN 106467892 A CN106467892 A CN 106467892A
- Authority
- CN
- China
- Prior art keywords
- culture fluid
- gas
- control system
- cell growth
- regulating valve
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/16—Hollow fibers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/18—External loop; Means for reintroduction of fermented biomass or liquid percolate
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/26—Means for regulation, monitoring, measurement or control, e.g. flow regulation of pH
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
- C12M41/32—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of substances in solution
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/40—Means for regulation, monitoring, measurement or control, e.g. flow regulation of pressure
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/44—Means for regulation, monitoring, measurement or control, e.g. flow regulation of volume or liquid level
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/48—Automatic or computerized control
Abstract
The present invention relates to technical field of cell culture, more particularly, to a kind of automatic cytological amplification system.The gas meltage being capable of in cell growth environment carries out automatization's real-time monitoring, provides for cell culture and stablizes growing environment.A kind of automatic cytological amplification system, including：By culture fluid conveyer device and the hollow fiber reactor of the circulation conduit connection of culture fluid, described hollow fiber reactor includes culture fluid passage and cell growth chamber；Described culture fluid conveyer device includes gas dissolver, described gas dissolver includes gas feed, culture fluid import and the culture fluid outlet on the housing of housing and setting, and described gas dissolver forms closed circuit by described culture fluid import and culture fluid outlet with described culture fluid passage；The gas offer device connecting with described gas feed, is provided with gas flow regulating valve between described gas offer device and described gas feed；The control system electrically connecting with described gas flow regulating valve.
Description
Technical field
The present invention relates to technical field of cell culture, more particularly, to a kind of automatic cytological amplification system.
Background technology
T cell has various biological function, such as direct killing target cell, and auxiliary or suppression B cell produce antibody, to spy
Specific Antigen and the former responsing reaction of mitogenesis and produce cytokine etc., ripe T cell is through blood distribution to outer
The thymus dependent areass of all immune organs are settled down, and can carry out recirculation through lymphatic vessel, peripheral blood and tissue fluid etc., play cell and exempt from
The function such as epidemic disease and immunomodulating.Successful incubation T cell, and by a large amount of for this cell injections in the patient, to strengthen immune system
Become a kind of possible.
In prior art, T cell is carried out cultivating and mainly adopt two methods, one kind is to use Tissue Culture Dish, high-grade
Laminar flow hood and CO2 gas incubator cultivated, this method less efficient it is impossible to meet clinical demand；Another kind is logical
Cross hollow fiber reactor cell is cultivated, one of which culture apparatuses, referring specifically to Fig. 1, are sequentially connected by mozzle
Main circulation pump 1, gas exchange pipe 2, culture tube 3 and culture fluid master library 4, described culture fluid master library 4 is provided with extraneous even
Logical steam vent；Wherein, described culture tube 3 is hollow fiber reactor, and described mozzle includes connecting described main circulation pump 1 He
Second mozzle 12 of the first mozzle 11 of described gas exchange pipe 2, the described gas exchange pipe 2 of connection and described culture tube 3,
Connect the 3rd mozzle 13 of described culture tube 3 and described culture fluid master library 4 and connect described culture fluid master library 4 and described master
4th mozzle 14 of circulating pump 1, described 3rd mozzle 13 is connected with waste tank 5 by the 5th mozzle 15, wherein, institute
State the shell that hollow fiber reactor 3 includes hollow fiber conduit and installs the cylinder of described hollow fiber conduit, described hollow
The inside and outside of fiber pipe forms culture fluid passage 31 and cell growth chamber 32 respectively, flows through institute in described culture fluid passage 31
State culture fluid, inoculating cell in described cell growth chamber 32, described cell growth chamber 32 and described 5th mozzle pass through the 6th
Mozzle 16 connects, and culture fluid can enter in described cell growth chamber 32 as described cell in the aperture through described hollow fiber conduit
There is provided nutrition that cell is cultivated, waste liquid produced by cell culture can drain in waste tank 5 through the 6th mozzle 16,
During this, cell culture system is closed system, is also difficult to in cell growth process in this closed system in prior art
The meltage of gas is (for example：Oxygen, carbon dioxide etc.) suitably adjusted so that cell is difficult under conditions of suitably stablizing
Growth, therefore, the regulation and control of cell growth environment constrain application in terms of cell culture for the hollow fiber reactor.
Content of the invention
Embodiments of the invention provide a kind of automatic cytological amplification system, are capable of the gas in cell growth environment
Body meltage carries out automatization's real-time monitoring, provides for cell culture and stablizes growing environment.
For reaching above-mentioned purpose, embodiments of the invention adopt the following technical scheme that：
On the one hand, the embodiment of the present invention provides a kind of automatic cytological amplification system, including：
By culture fluid conveyer device and the hollow fiber reactor of the circulation conduit connection of culture fluid, described hollow is fine
Dimension reactor includes culture fluid passage and cell growth chamber；Described culture fluid conveyer device includes gas dissolver, described gas
Dissolvers include gas feed, culture fluid import and the culture fluid outlet on the housing of housing and setting, described gas
Dissolvers form closed circuit by described culture fluid import and culture fluid outlet with described culture fluid passage；
The gas offer device being connected with described gas feed, is set between described gas offer device and described gas feed
It is equipped with gas flow regulating valve；
The control system electrically connecting with described gas flow regulating valve；
Described culture fluid conveyer device is used for conveying culture fluid to described culture fluid passage, and described control system is used for controlling
The flow that the aperture of described gas flow regulating valve is passed through gas to described gas offer device into described gas dissolver enters
Row is adjusted.
Preferably, it is provided with hollow fiber conduit in the housing of described gas dissolver, described hollow fiber conduit is by described shell
Internal space is divided into culture fluid and flows through side and gas flow side, and described culture fluid flows through side and described culture fluid import and described
Culture fluid outlet, described gas flow side is connected with described gas feed.
Optionally, described gas offer device includes dioxide bottle and oxygen gas cylinder, described dioxide bottle with
It is provided with first flow regulating valve between described gas feed, between described oxygen gas cylinder and described gas feed, be provided with second
Flow control valve, described control system is electrically connected with described first flow regulating valve and described second flow regulating valve, for right
The flow of the carbon dioxide and oxygen being passed through in described gas dissolver is adjusted.
Further, described control system is used for obtaining the consumption models of carbon dioxide and the consumption models of oxygen, and root
According to the consumption models of described carbon dioxide, the aperture of described first flow regulating valve is adjusted so that the pH value of culture fluid is protected
Hold in the first preset range, the consumption models according to described oxygen are adjusted to the aperture of described second flow regulating valve,
The dissolved oxygen amount making culture fluid is maintained in the second preset range.
Optionally, described circulation conduit is provided with pH detector and dissolved oxygen amount detector, described control system with described
PH detector and the electrical connection of described dissolved oxygen amount detector, described control system is used for the pH signal sending according to described pH detector
The aperture of described first flow regulating valve is adjusted so that pH value is maintained in the first preset range；According to described dissolved oxygen
The dissolved oxygen amount signal that amount detector sends is adjusted to the aperture of described second flow regulating valve so that dissolved oxygen amount is maintained at the
In two preset range.
Preferably, described culture fluid conveyer device also includes circulating pump, the outfan of described circulating pump and described culture fluid
The outlet of inlet communication, input and described culture fluid passage, described circulating pump is electrically connected with described control system；
Described system also includes pressure extended case；It is filled with gas, the bottom of described pressure extended case in described pressure extended case
Portion is connected with described culture fluid passage or described cell growth chamber, and described control system is additionally operable to control described circulating pump will train
Nutrient solution flows through described culture fluid passage in the form of impulse wave so that the pressure in described culture fluid passage and described cell growth chamber
The positive and negative alternate of difference, described pressure extended case is used for making described culture fluid passage and described cell by gas volume change
The pressure balance of growth chamber.
Further, described cell growth chamber is provided with first pressure sensor, and the culture fluid of described gas dissolver goes out
Be additionally provided with second pressure sensor on circulation conduit mouthful and the import of described culture fluid passage between, described control system with
Described first pressure sensor and the electrical connection of described second pressure sensor, described control system is additionally operable to obtain described cell life
The Metabolites Concentration model of growth in long chamber, and sent according to described first pressure sensor and described second pressure sensor
Circulating pump described in Stress control conveys culture fluid so that described cell growth chamber with predetermined pulse model to described culture fluid passage
Metabolites Concentration be maintained in the 3rd preset range.
Optionally, described system also includes：Waste liquid delivery pump and waste tank；
Described culture fluid conveyer device also includes the training being arranged between described hollow fiber reactor and described circulating pump
Nutrient solution surge tank, the import of described culture fluid surge tank and the outlet of described culture fluid passage, export and described circulating pump
Input connection；
Pipeline between the input of described waste liquid delivery pump and described culture fluid surge tank and described culture fluid passage is even
Logical, outfan is connected with described waste tank, and described control system is electrically connected with described waste liquid delivery pump；
Described control system is additionally operable to obtain the model of growth of the products of cellular metabolism of described culture fluid passage however, it is determined that thin
Difference between the concentration of products of cellular metabolism of the predetermined threshold value of born of the same parents' metabolite and described culture fluid passage is less than or equal to the
During one predetermined threshold value, then control described waste liquid delivery pump to open, the culture fluid of described culture fluid passage is delivered to waste tank
In.
Preferably, described system also includes sealant supplement cylinder and culture fluid delivery pump；
Described cell growth chamber also includes import, and the outlet of described sealant supplement cylinder is with the input of described culture fluid delivery pump even
Logical, the inlet communication in the outfan of described culture fluid delivery pump and described cell growth chamber, the culture fluid of described gas dissolver
It is additionally provided with temperature sensor, described sealant supplement cylinder and described culture fluid on outlet and the circulation conduit of described culture fluid channel connection
Be additionally provided with heater on the pipeline of delivery pump connection, described control system and described culture fluid delivery pump, temperature sensor and
Heater electrically connects；
Described control system is additionally operable to obtain the temperature signal that described temperature sensor sends, and described culture fluid is conveyed
The open and close of pump and described heater are controlled so that the temperature of described culture fluid passage is maintained at the 4th preset range
Interior.
Preferably, the pipeline of inlet communication in described culture fluid delivery pump and described cell growth chamber is additionally provided with the 3rd
Pressure transducer, described control system is electrically connected with described 3rd pressure transducer, and described control system is additionally operable to according to described
The speed that the pressure signal that 3rd pressure transducer sends conveys culture fluid to described culture fluid delivery pump is controlled so that institute
The speed stating culture fluid delivery pump conveying culture fluid is maintained in the 5th preset range.
The embodiment of the present invention provides a kind of automatic cytological amplification system, by arranging gas offer device, and will be described
Gas offer device is connected with described air inlet, the gas required for can providing for the gas flow side of described gas dissolver
Body, and, control the aperture of described gas flow regulating valve by control system, can be to the gas flowing through described gas flow side
The flow of body is controlled, and provides stable gas for cell culture such that it is able to the gas meltage in described culture fluid
Carry out Automated condtrol so that the culture of culture fluid suitable cell, provide stable growing environment for cell culture.
Brief description
A kind of structural representation of cell cycle culture systems that Fig. 1 provides for prior art；
Fig. 2 is a kind of structural representation of automatic cytological amplification system provided in an embodiment of the present invention；
Fig. 3 is the structural representation of another kind automatic cytological amplification system provided in an embodiment of the present invention；
Fig. 4 is the structural representation of another kind automatic cytological amplification system provided in an embodiment of the present invention；
Fig. 5 is the structural representation of another kind automatic cytological amplification system provided in an embodiment of the present invention；
Fig. 6 is the structural representation of another kind automatic cytological amplification system provided in an embodiment of the present invention；
Fig. 7 is the structural representation of another kind automatic cytological amplification system provided in an embodiment of the present invention.
Specific embodiment
Below in conjunction with the accompanying drawings to a kind of automatic cytological amplification system provided in an embodiment of the present invention and its cell culture side
Method is described in detail.
In describing the invention it is to be understood that term " " center ", " on ", D score, "front", "rear", " left ",
The orientation of instruction such as " right ", " vertical ", " level ", " top ", " bottom ", " interior ", " outward " or position relationship are based on shown in the drawings
Orientation or position relationship, be for only for ease of description the present invention and simplify description, rather than instruction or hint indication device or
Element must have specific orientation, with specific azimuth configuration and operation, be therefore not considered as limiting the invention.
Term " first ", " second " be only used for describe purpose, and it is not intended that instruction or hint relative importance or
The implicit quantity indicating indicated technical characteristic.Thus, define " first ", the feature of " second " can be expressed or implicit
Ground includes one or more this feature.
It should be noted that a kind of automatic cytological amplification system provided in an embodiment of the present invention is applied to any cell
Amplification, is particularly suited for the amplification cultivation of suspension cell and attached cell.
On the one hand, the embodiment of the present invention provides a kind of automatic cytological amplification system, referring to Fig. 2, including：
By culture fluid conveyer device and the hollow fiber reactor 3 of the circulation conduit connection of culture fluid, described hollow
Fiber reactor 3 includes culture fluid passage 31 and cell growth chamber 32；Described culture fluid conveyer device includes gas dissolver 2,
Described gas dissolver 2 includes gas feed, culture fluid import and the culture fluid outlet on the housing of housing and setting,
Described gas dissolver 2 forms closed circuit by described culture fluid import and culture fluid outlet with described culture fluid passage 31；
Gas offer device (shown in figure 01 and 02), described gas offer device and the institute connecting with described gas feed
State and between gas feed, be provided with gas flow regulating valve (shown in figure a and b)；
The control system 100 electrically connecting with described gas flow regulating valve；
Described culture fluid conveyer device is used for conveying culture fluid to described culture fluid passage 31, and described control system 100 is used
In control described gas flow regulating valve aperture to described gas offer device to described gas dissolver 2 in be passed through gas
Flow is adjusted.
The embodiment of the present invention provides a kind of automatic cytological amplification system, by arranging gas offer device, and will be described
Gas offer device is connected with described air inlet, the gas required for can providing for the gas flow side of described gas dissolver 2
Body, and, control the aperture of described gas flow regulating valve (a and b) by control system 100, can be to flowing through described gas
The flow of the gas of dissolvers 2 is controlled, and provides certain gas flow for cell culture such that it is able to described culture fluid
In gas meltage carry out Automated condtrol so that the culture of culture fluid suitable cell, provide stable growth for cell culture
Environment.
In one embodiment of the invention, referring to Fig. 3, in the housing of described gas dissolver 2, it is provided with hollow fiber conduit 21,
Space in described housing is divided into culture fluid and flows through side 22 and gas flow side 23, described culture fluid by described hollow fiber conduit 21
Flow through side 22 and described culture fluid import and described culture fluid outlet, described gas flow side 23 is with described gas feed even
Logical.
By arranging hollow fiber conduit 21 so that the gas in gas flow side 23 is spread by described hollow fiber conduit 21
Enter described culture fluid and flow through side 22, can be fully contacted with culture fluid, improve the meltage of gas.
In another embodiment of the present invention, described gas offer device includes dioxide bottle 01 and oxygen gas cylinder 02,
It is provided with first flow regulating valve a, described oxygen gas cylinder 02 and institute between described dioxide bottle 01 and described gas feed
State and between gas feed, be provided with second flow regulating valve b, described control system 100 and described first flow regulating valve a and institute
State the electrical connection of second flow regulating valve b, for carrying out to the flow of the carbon dioxide and oxygen being passed through in described gas dissolver 2
Adjust.
By arranging dioxide bottle and oxygen gas cylinder, carbon dioxide and oxygen can be provided for described culture fluid
Gas, such that it is able to provide carbon dioxide and oxygen for described culture fluid, meets cell culture demand, meanwhile, by described control
System is adjusted to the gas flow being passed through in described gas dissolver 2, and the meltage of carbon dioxide and oxygen can be entered
Row controls.
In one embodiment of the invention, described control system 100 is used for obtaining the consumption models of carbon dioxide and oxygen
Consumption models, and according to the consumption models of described carbon dioxide, the aperture of described first flow regulating valve a is adjusted so that
The pH value of culture fluid is maintained in the first preset range, and the consumption models according to described oxygen are to described second flow regulating valve b
Aperture be adjusted so that the dissolved oxygen amount of culture fluid is maintained in the second preset range.
Because, in cell cultivation process, carbon dioxide primarily serves the effect adjusting pH value, can be according to any time
The situation of change of the pH value of the culture fluid and carbon dioxide dissolubility in described culture fluid to control the logical of described carbon dioxide
Enter amount such that it is able to real-time monitoring is carried out to the pH value of culture fluid；Meanwhile, cell can consume a small amount of oxygen during training oxygen
Gas, can control the logical of oxygen according to the keto consumpting speed of the cell of any time and oxygen dissolubility in described culture fluid
Enter amount such that it is able to timely dissolved oxygen, control dissolved oxygen amount.
In one embodiment of the invention, referring to Fig. 3, described circulation conduit is provided with pH detector 03 and dissolved oxygen amount detection
Device 04, described control system 100 is electrically connected with described pH detector 03 and dissolved oxygen amount detector 04, and described control system 100 is used
The aperture of described first flow regulating valve a is adjusted so that pH value in the pH signal being sent according to described pH detector 03
It is maintained in the first preset range；According to the dissolved oxygen amount signal that described dissolved oxygen amount detector 04 sends, described second flow is adjusted
The aperture of valve b is adjusted so that dissolved oxygen amount is maintained in the second preset range.
By described pH detector 03 and described dissolved oxygen amount detector 04 to the pH value of the culture fluid in circulating system and molten
Oxygen amount carries out real-time detection, and by described control system 100, the flow of carbon dioxide and the flow of oxygen is adjusted in time
Section is such that it is able to the pH value to the culture fluid in circulating system and dissolved oxygen amount carry out monitor in real time it is ensured that providing suitably for cell
Growing environment.
In one embodiment of the invention, referring to Fig. 4, described culture fluid conveyer device also includes circulating pump 1, described circulating pump
1 outfan and described culture fluid inlet communication, the outlet of input and described culture fluid passage 31, described circulating pump 1
Electrically connect with described control system 100；
Described cell culture blood circulation also includes pressure extended case 6；It is filled with gas in described pressure extended case 6, described
The bottom of pressure extended case 6 is connected with described culture fluid passage 31 or described cell growth chamber 32, and described control system 100 is also
For controlling described circulating pump 1 that culture fluid is flowed through in the form of impulse wave described culture fluid passage 31 so that described culture fluid
The positive and negative alternate of pressure differential in passage 31 and described cell growth chamber 32, described pressure extended case 6 is used for by gas volume
Change makes described culture fluid passage 31 and the pressure balance in described cell growth chamber 32.
In embodiments of the present invention, by arrange pressure extended case 6, and by described control system control circulating pump 1 with
The form conveying culture fluid of impulse wave is so that the pressure differential in described culture fluid passage 31 and described cell growth chamber 32 is positive and negative alternately
Change.When described pressure differential is timing, the culture fluid of described culture fluid passage 31 enters described cell growth chamber 32, pressure extended
The gas volume of case 6 is compressed, until pressure balance.When described pressure differential is negative, the waste liquid in described cell growth chamber 32
Enter described culture fluid passage 31, the gas volume of pressure extended case 6 increases, until pressure balance, thus realize culture fluid and
The exchange of waste liquid, so, in waste liquid, the favourable nutrient substance of cell growth can be cultured liquid band to enter circulating system is cell
Used additionally it is possible to constantly carry out culture fluid renewal, reduce culture fluid consumption.
For different cell categories, the exchange capacity of culture fluid and waste liquid is also different, and is applied to described training
Pressure differential between nutrient solution passage 31 and described cell growth chamber 32 would also vary from, with described attached cell and described suspension
Illustrate as a example cell, when cultivating to suspension cell, directly suspension cell can be inoculated in cell growth chamber 32
Interior, cell is in suspended state, and when cultivating to attached cell, needs the surface in cell growth chamber 32 to carry out pre- place
Reason, for example, when the inside of described hollow fiber conduit is cell growth chamber 32, then lays on the inwall of described hollow fiber conduit
One layer of albumen is so that the inwall that cell can be attached to described hollow fiber conduit carries out adhere-wall culture.Vice versa.Therefore, lead to
In order to realize the exchange of culture fluid and waste liquid, described culture fluid passage 31 and described cell growth chamber 32 during often attached cell culture
Between pressure differential be slightly larger than suspension cell culture when pressure differential.
In another embodiment of the present invention, referring to Fig. 4, described cell growth chamber 32 is provided with first pressure sensor 05,
It is additionally provided with the on circulation conduit between the culture fluid outlet of described gas dissolver 2 and the import of described culture fluid passage 31
Two pressure transducers 06, described control system 100 and described first pressure sensor 05 and described second pressure sensor 06 electricity
Connect, described control system 100 is additionally operable to obtain the Metabolites Concentration model of growth in described cell growth chamber 32, and according to institute
State the circulating pump 1 described in Stress control that first pressure sensor 05 and described second pressure sensor 06 send with predetermined pulse mould
Type convey culture fluid to described culture fluid passage 31 so that the Metabolites Concentration in described cell growth chamber 32 to be maintained at the 3rd pre-
If in scope.
While by realizing exchanging to culture fluid and waste liquid, control described circulating pump 1 by culture fluid with predetermined pulse mould
Type flows through described culture fluid passage 31, and the exchange capacity of culture fluid and waste liquid and exchange frequency can be controlled, so that
The Metabolites Concentration of described cell growth intracavity is unlikely to too high and cell growth has a negative impact.Exemplary,
The impulse wave of described predetermined pulse model can be rectangular wave, sine wave etc., the crest of described predetermined pulse model and trough it
Between difference also changeable.The crest of the exchange capacity in the unit interval of culture fluid and waste liquid and described predetermined pulse model and
The size of trough and pulse frequency all have relation, can rule of thumb or Theoretical Calculation is determining described predetermined pulse model
Concrete form, as long as enabling to culture fluid and waste liquid is exchanged in time, reduce the metabolism in described cell growth chamber 32
Production concentration.
Also, it should be noted with the culture of cell, metabolite is on the increase so that described cell growth intracavity
Culture fluid viscosity increases, in order to improve the exchange effect of culture fluid and waste liquid, can also be by being gradually shortened described impulse wave
Time cycle or/and increase pressure differential between described culture fluid passage and described cell growth chamber and to realize.
In another embodiment of the present invention, described control system 100 is additionally operable to receive described second pressure sensor 06 anti-
The pressure signal of feedback, and according to the pressure signal of feedback, the pulse signal of described circulating pump 1 is corrected.
In embodiments of the present invention, by closed loop control so that described circulating pump 1 is transported with stable pulse signal
OK, the pressure differential steady change and to described culture fluid passage 31 and described cell growth chamber 32.
In one embodiment of the invention, referring to Fig. 5, the top of described pressure extended case 6 is provided with the first pipeline 61, described
It is provided with the 3rd valve c on first pipeline 61；
It is provided with the first liquid level outside described pressure extended case 6 from top to bottom respectively to pass at the first height and the second height
Sensor 07 and the second liquid level sensor 08, described control system 100 is passed with described first liquid level sensor 07, the second liquid level respectively
Sensor 08 and the 3rd valve c electrical connection, described control system 100 is used for culture fluid is detected in described first liquid level sensor 07
Liquid level reach control during the first height described 3rd valve c open, the gas into described pressure extended case 6, described
Second liquid level sensor 08 controls described 3rd valve c to open when liquid level arrival second height of culture fluid is detected, from
Portion gas are released so that the liquid level of described culture fluid is maintained at the first height and the second height in described pressure extended case 6
Between degree.
In embodiments of the present invention, by the first liquid level sensor 07 and described second liquid level sensor 08, can be to institute
State the gas flow in pressure extended case 6 to be monitored in time so that the gas volume in described pressure extended case 6 is maintained at necessarily
In the range of, it is easy to the exchange capacity of culture fluid and waste liquid is controlled.
In one embodiment of the invention, described system also includes waste tank 5 and waste liquid delivery pump 51,
Described culture fluid conveyer device also includes being arranged between described hollow fiber reactor 3 and described circulating pump 1
Culture fluid surge tank 4, the outlet of the import of described culture fluid surge tank 4 and described culture fluid passage 31, outlet with described
The input connection of circulating pump 1；
Pipe between the input of described waste liquid delivery pump 51 and described culture fluid surge tank 4 and described culture fluid passage 31
Road connects, and outfan is connected with described waste tank 5, and described control system 100 is electrically connected with described waste liquid delivery pump 51；
Described control system 100 is additionally operable to obtain the model of growth of the products of cellular metabolism of described culture fluid passage 31, if
Determine that the difference between the predetermined threshold value of products of cellular metabolism and the concentration of products of cellular metabolism of described culture fluid passage 31 is little
When equal to the first predetermined threshold value, then described waste liquid delivery pump 51 is controlled to open, will be defeated for the culture fluid of described culture fluid passage 31
Deliver in waste tank 5.
Wherein, the model of growth of the products of cellular metabolism of described culture fluid passage 31 can be obtained by experience it is also possible to
Obtained by experiment, when being obtained by experiment, change curve at any time in section for the products of cellular metabolism can be drawn, lead to
Cross this change curve, any time can be calculated according to the growth rate of the Metabolites Concentration of any time and any time period
Metabolite concentration however, it is determined that difference between the concentration of described metabolite and predetermined threshold value is less than or equal to first presets
During threshold value, then control described waste liquid delivery pump 51 to open, culture fluid is delivered in described waste tank 5.With cell growth
Carrying out, the continuous exchange of culture fluid and waste liquid, when the Metabolites Concentration in the culture fluid of described culture fluid passage 31 is higher,
When being unsuitable for cell growth, can be drained in described waste tank 5.
In another embodiment of the present invention, referring to Fig. 6, described system also includes sealant supplement cylinder 7 and culture fluid delivery pump 71；
Described cell growth chamber 32 also includes import, and the outlet of described sealant supplement cylinder 7 is defeated with described culture fluid delivery pump 71
Enter end connection, the inlet communication in the outfan of described culture fluid delivery pump 71 and described cell growth chamber 32, described gas dissolving
It is additionally provided with temperature sensor 09, described benefit on the circulation conduit that the culture fluid outlet of device 2 is connected with described culture fluid passage 31
Heater 8, described control system 100 and described training are additionally provided with the pipeline that flow container 7 is connected with described culture fluid delivery pump 71
Nutrient solution delivery pump 71, temperature sensor 09 and heater 8 electrically connect；
Described control system 100 is additionally operable to obtain the temperature signal that described temperature sensor 09 sends, and to described culture
The open and close of liquid delivery pump 71 and described heater 8 are controlled so that the temperature of described culture fluid passage 31 is maintained at
In 4th preset range.
During Cyclic culture, in the temperature and described hollow fiber reactor 3 that described culture fluid passage 31 is embodied
The temperature in portion is basically identical, by detecting to the temperature of circulating system, and passes through to control institute according to detected temperature
State culture fluid delivery pump 71 conveying culture fluid and it is heated or not heater, described culture fluid delivery pump 71 can be conveyed
The temperature of culture fluid be adjusted, and the friendship by described culture fluid passage 31 and the culture fluid in described cell growth chamber 32
Change, the temperature of described circulating system can be adjusted so that cell grows in the environment of proper temperature.
In another embodiment of the present invention, the inlet communication in described culture fluid delivery pump 71 and described cell growth chamber 32
3rd pressure transducer 010 is additionally provided with pipeline, described control system 100 is electrically connected with described 3rd pressure transducer 010,
The pressure signal that described control system 100 is additionally operable to according to described 3rd pressure transducer 010 sends conveys to described culture fluid
The speed that pump 71 conveys culture fluid is controlled so that the speed that described 1 culture fluid delivery pump 71 conveys culture fluid is maintained at the
In five preset range.
In embodiments of the present invention, culture fluid is conveyed to described culture fluid delivery pump 72 by the 3rd pressure transducer 010
Pressure carry out in time detection, can be by described control system 100 to described culture fluid passage 31 and described cell growth chamber
32 pressure differential is adjusted such that it is able to be controlled to the exchange frequency and exchange capacity of culture fluid and waste liquid, realizes to institute
State the accurate regulation and control of the temperature of circulating system.
In one embodiment of the invention, referring to Fig. 7, described system also includes fluid infusion pump 72, the input of described fluid infusion pump 72
End connects with described sealant supplement cylinder 7, the inlet communication of outfan and described culture fluid surge tank 4, and described culture fluid surge tank 4 is from upper
It is provided with the 3rd liquid level sensor 011 and the 4th liquid level sensor 012 to lower at third height and the 4th height respectively, described
Control system 100 is additionally operable to the liquid level that described 3rd liquid level sensor 011 detects culture fluid and reaches third height time control
Make described fluid infusion pump 72 to close, stop to fluid infusion in described culture fluid surge tank 4；Detect in described 4th liquid level sensor 010
To culture fluid liquid level reach four height when control described fluid infusion pump 72 to open, in described culture fluid surge tank 4 mend
Liquid so that in described culture fluid surge tank 4 liquid level of culture fluid be maintained between third height and the 4th height.
When fluid infusion is carried out to described culture fluid passage 32 and/or described cell growth chamber 33 by described sealant supplement cylinder 7, lead to
Cross the liquid level to described culture fluid surge tank 4 to be monitored, it can be avoided that culture fluid is not enough in described culture fluid surge tank 4
Cause cycle interruption it is also possible to preventing culture fluid in described culture fluid surge tank 4 excessive and overflowing.
The above, the only specific embodiment of the present invention, but protection scope of the present invention is not limited thereto, and any
Those familiar with the art the invention discloses technical scope in, change or replacement can be readily occurred in, all should contain
Cover within protection scope of the present invention.Therefore, protection scope of the present invention should be defined by described scope of the claims.
Claims (10)
1. a kind of automatic cytological amplification system is it is characterised in that include：
By culture fluid conveyer device and the hollow fiber reactor of the circulation conduit connection of culture fluid, described doughnut is anti-
Device is answered to include culture fluid passage and cell growth chamber；Described culture fluid conveyer device includes gas dissolver, described gas dissolving
Device includes gas feed, culture fluid import and the culture fluid outlet on the housing of housing and setting, described gas dissolving
Device forms closed circuit by described culture fluid import and culture fluid outlet with described culture fluid passage；
The gas offer device being connected with described gas feed, is provided between described gas offer device and described gas feed
Gas flow regulating valve；
The control system electrically connecting with described gas flow regulating valve；
Described culture fluid conveyer device is used for conveying culture fluid to described culture fluid passage, and described control system is used for controlling described
The flow that the aperture of gas flow regulating valve is passed through gas to described gas offer device into described gas dissolver is adjusted
Section.
2. automatic cytological amplification system according to claim 1 it is characterised in that
It is provided with hollow fiber conduit, space in described housing is separated by described hollow fiber conduit in the housing of described gas dissolver
Flow through side and gas flow side for culture fluid, described culture fluid is flowed through side and connected with described culture fluid import and the outlet of described culture fluid
Logical, described gas flow side is connected with described gas feed.
3. automatic cytological amplification system according to claim 1 it is characterised in that
Described gas offer device includes dioxide bottle and oxygen gas cylinder, described dioxide bottle and described gas feed
Between be provided with first flow regulating valve, be provided with second flow regulating valve between described oxygen gas cylinder and described gas feed,
Described control system is electrically connected with described first flow regulating valve and described second flow regulating valve, for being passed through described gas
The flow of the carbon dioxide and oxygen in dissolvers is adjusted.
4. automatic cytological amplification system according to claim 3 it is characterised in that
Described control system is used for obtaining the consumption models of carbon dioxide and the consumption models of oxygen, and according to described carbon dioxide
Consumption models the aperture of described first flow regulating valve is adjusted so that the pH value of culture fluid is maintained at the first default model
In enclosing, the consumption models according to described oxygen the aperture of described second flow regulating valve is adjusted so that culture fluid molten
Oxygen amount is maintained in the second preset range.
5. automatic cytological amplification system according to claim 3 it is characterised in that
PH detector and dissolved oxygen amount detector, described control system and described pH detector and institute are provided with described circulation conduit
State dissolved oxygen amount detector electrical connection, described control system be used for according to described pH detector send pH signal to described first-class
The aperture of adjustable valve is adjusted so that pH value is maintained in the first preset range；Sent according to described dissolved oxygen amount detector
Dissolved oxygen amount signal the aperture of described second flow regulating valve is adjusted so that dissolved oxygen amount is maintained at the second preset range
Interior.
6. automatic cytological amplification system according to claim 1 it is characterised in that
Described culture fluid conveyer device also includes circulating pump, the outfan of described circulating pump and described culture fluid inlet communication, defeated
Enter the outlet at end and described culture fluid passage, described circulating pump is electrically connected with described control system；
Described system also includes pressure extended case；Be filled with gas in described pressure extended case, the bottom of described pressure extended case with
Described culture fluid passage or the connection of described cell growth chamber, described control system is additionally operable to control described circulating pump by culture fluid
Flow through described culture fluid passage in the form of impulse wave, described pressure extended case is used for making described training by gas volume change
Nutrient solution passage and the pressure balance in described cell growth chamber.
7. automatic cytological amplification system according to claim 6 it is characterised in that
Described cell growth chamber is provided with first pressure sensor, culture fluid outlet and the described culture fluid of described gas dissolver
Second pressure sensor is additionally provided with the circulation conduit between the import of passage, described control system is passed with described first pressure
Sensor and the electrical connection of described second pressure sensor, described control system is additionally operable to obtain the metabolite in described cell growth chamber
Concentration model of growth, and follow according to the Stress control that described first pressure sensor and described second pressure sensor send
Ring pump conveys culture fluid so that the Metabolites Concentration in described cell growth chamber with predetermined pulse model to described culture fluid passage
It is maintained in the 3rd preset range.
8. automatic cytological amplification system according to claim 6 it is characterised in that
Described system also includes：Waste liquid delivery pump and waste tank；
Described culture fluid conveyer device also includes the culture fluid being arranged between described hollow fiber reactor and described circulating pump
Surge tank, the import of described culture fluid surge tank and the outlet of described culture fluid passage, export defeated with described circulating pump
Enter end connection；
Pipeline communication between the input of described waste liquid delivery pump and described culture fluid surge tank and described culture fluid passage, defeated
Go out end to connect with described waste tank, described control system is electrically connected with described waste liquid delivery pump；
Described control system is additionally operable to obtain the model of growth of the products of cellular metabolism of described culture fluid passage however, it is determined that cell generation
The difference thanked between the predetermined threshold value of product and the concentration of products of cellular metabolism of described culture fluid passage is pre- less than or equal to first
If during threshold value, then control described waste liquid delivery pump to open, the culture fluid of described culture fluid passage is delivered in waste tank.
9. automatic cytological amplification system according to claim 1 it is characterised in that
Described system also includes sealant supplement cylinder and culture fluid delivery pump；
Described cell growth chamber also includes import, and the outlet of described sealant supplement cylinder is connected with the input of described culture fluid delivery pump,
The outfan of described culture fluid delivery pump and the inlet communication in described cell growth chamber, the culture fluid outlet of described gas dissolver
It is additionally provided with temperature sensor with the circulation conduit of described culture fluid channel connection, described sealant supplement cylinder is conveyed with described culture fluid
It is additionally provided with heater, described control system and described culture fluid delivery pump, temperature sensor and heating on the pipeline of pump connection
Device electrically connects；
Described control system is additionally operable to obtain the temperature signal that described temperature sensor sends, and to described culture fluid delivery pump and
The open and close of described heater are controlled so that the temperature of described culture fluid passage is maintained in the 4th preset range.
10. automatic cytological amplification system according to claim 9 it is characterised in that
It is additionally provided with the 3rd pressure transducer on the pipeline of inlet communication in described culture fluid delivery pump and described cell growth chamber,
Described control system is electrically connected with described 3rd pressure transducer, and described control system is additionally operable to according to described 3rd pressure sensing
The speed that the pressure signal that device sends conveys culture fluid to described culture fluid delivery pump is controlled so that described culture fluid conveys
The speed that pump conveys culture fluid is maintained in the 5th preset range.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610800908.XA CN106467892B (en) | 2016-09-01 | 2016-09-01 | A kind of automatic cytological amplification system |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610800908.XA CN106467892B (en) | 2016-09-01 | 2016-09-01 | A kind of automatic cytological amplification system |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106467892A true CN106467892A (en) | 2017-03-01 |
| CN106467892B CN106467892B (en) | 2018-12-18 |
Family
ID=58230426
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610800908.XA Active CN106467892B (en) | 2016-09-01 | 2016-09-01 | A kind of automatic cytological amplification system |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106467892B (en) |
Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107012094A (en) * | 2017-05-26 | 2017-08-04 | 奥凯（苏州）生物技术有限公司 | A kind of module group unit cell expansion system |
| CN107043734A (en) * | 2017-05-26 | 2017-08-15 | 奥凯（苏州）生物技术有限公司 | A kind of automatic collection method of the cell of cell expansion system |
| CN107189975A (en) * | 2017-05-26 | 2017-09-22 | 奥凯（苏州）生物技术有限公司 | A kind of cell collection method of closed cell expansion system |
| CN107904167A (en) * | 2017-11-15 | 2018-04-13 | 中国航天员科研训练中心 | The automatic amplification cultivation equipment of cell |
| CN107904170A (en) * | 2017-12-18 | 2018-04-13 | 上海白泽医疗器械有限公司 | Media to cell culture modules and cell culture system |
| CN108300661A (en) * | 2018-02-13 | 2018-07-20 | 京东方科技集团股份有限公司 | Culture apparatus and culture device |
| CN109913410A (en) * | 2019-04-19 | 2019-06-21 | 华子昂 | The emulation cultural method of stem cell |
| CN110404112A (en) * | 2018-04-26 | 2019-11-05 | 西安新长安再生医学科技研发有限公司 | A kind of preparation method of self beauty biomaterial and self beauty biomaterial |
| CN110499252A (en) * | 2018-05-18 | 2019-11-26 | 华赛公司 | Automatic cytological culture |
| CN111218403A (en) * | 2019-03-12 | 2020-06-02 | 华道(上海)生物医药有限公司 | Totally enclosed cell culture method |
| CN112940933A (en) * | 2021-01-29 | 2021-06-11 | 上海睿钰生物科技有限公司 | In-vitro life-sustaining perfusion culture system and control method thereof |
| WO2021114129A1 (en) * | 2019-12-11 | 2021-06-17 | 中国科学院深圳先进技术研究院 | In vitro experimental model for research of mechanism of tumor perineural invasion |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1290744A (en) * | 1999-12-09 | 2001-04-11 | 中国科学院力学研究所 | Spacial dialyzing counterflow cell cultivator and cultivating method |
| CN101486967A (en) * | 2009-02-13 | 2009-07-22 | 中国人民解放军第三军医大学第一附属医院 | Culture fluid gas/liquid and liquid/liquid exchanger for tissue engineering tissue bionic cultivation |
| CN201737949U (en) * | 2010-07-12 | 2011-02-09 | 中国人民解放军第三军医大学第一附属医院 | Cell culturing device capable of periodically converting positive pressure and negative pressure |
| CN203048948U (en) * | 2012-10-17 | 2013-07-10 | 浙江赛尚医药科技有限公司 | Cell culture piping device and cell automated culture system |
| CN103589808A (en) * | 2013-11-11 | 2014-02-19 | 中国人民解放军第三军医大学第一附属医院 | Automatic control method and system for pH/oxygen partial pressure/partial pressure of carbon dioxide of cell and tissue culture solution |
-
2016
- 2016-09-01 CN CN201610800908.XA patent/CN106467892B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1290744A (en) * | 1999-12-09 | 2001-04-11 | 中国科学院力学研究所 | Spacial dialyzing counterflow cell cultivator and cultivating method |
| CN101486967A (en) * | 2009-02-13 | 2009-07-22 | 中国人民解放军第三军医大学第一附属医院 | Culture fluid gas/liquid and liquid/liquid exchanger for tissue engineering tissue bionic cultivation |
| CN201737949U (en) * | 2010-07-12 | 2011-02-09 | 中国人民解放军第三军医大学第一附属医院 | Cell culturing device capable of periodically converting positive pressure and negative pressure |
| CN203048948U (en) * | 2012-10-17 | 2013-07-10 | 浙江赛尚医药科技有限公司 | Cell culture piping device and cell automated culture system |
| CN103589808A (en) * | 2013-11-11 | 2014-02-19 | 中国人民解放军第三军医大学第一附属医院 | Automatic control method and system for pH/oxygen partial pressure/partial pressure of carbon dioxide of cell and tissue culture solution |
Cited By (20)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107012094B (en) * | 2017-05-26 | 2019-03-22 | 奥凯（苏州）生物技术有限公司 | A kind of module group unit cell expansion system |
| CN107043734A (en) * | 2017-05-26 | 2017-08-15 | 奥凯（苏州）生物技术有限公司 | A kind of automatic collection method of the cell of cell expansion system |
| CN107189975A (en) * | 2017-05-26 | 2017-09-22 | 奥凯（苏州）生物技术有限公司 | A kind of cell collection method of closed cell expansion system |
| CN107189975B (en) * | 2017-05-26 | 2020-12-25 | 奥凯（苏州）生物技术有限公司 | Cell collection method of closed cell amplification system |
| CN107043734B (en) * | 2017-05-26 | 2020-12-25 | 奥凯（苏州）生物技术有限公司 | Automatic cell collection method of cell amplification system |
| CN107012094A (en) * | 2017-05-26 | 2017-08-04 | 奥凯（苏州）生物技术有限公司 | A kind of module group unit cell expansion system |
| CN107904167A (en) * | 2017-11-15 | 2018-04-13 | 中国航天员科研训练中心 | The automatic amplification cultivation equipment of cell |
| CN107904167B (en) * | 2017-11-15 | 2023-06-06 | 中国航天员科研训练中心 | Automatic cell expansion culture equipment |
| CN107904170A (en) * | 2017-12-18 | 2018-04-13 | 上海白泽医疗器械有限公司 | Media to cell culture modules and cell culture system |
| WO2019157826A1 (en) * | 2018-02-13 | 2019-08-22 | 京东方科技集团股份有限公司 | Biological sheet culture device and biological sheet culture equipment |
| CN108300661A (en) * | 2018-02-13 | 2018-07-20 | 京东方科技集团股份有限公司 | Culture apparatus and culture device |
| CN110404112A (en) * | 2018-04-26 | 2019-11-05 | 西安新长安再生医学科技研发有限公司 | A kind of preparation method of self beauty biomaterial and self beauty biomaterial |
| CN110404112B (en) * | 2018-04-26 | 2022-03-04 | 西安新长安再生医学科技研发有限公司 | Preparation method of self-body beautifying biological material and self-body beautifying biological material |
| CN110499252A (en) * | 2018-05-18 | 2019-11-26 | 华赛公司 | Automatic cytological culture |
| CN110499252B (en) * | 2018-05-18 | 2023-04-18 | 华赛公司 | Automated cell culture |
| CN111334427A (en) * | 2019-03-12 | 2020-06-26 | 华道(上海)生物医药有限公司 | Totally-enclosed cell culture system |
| CN111218403A (en) * | 2019-03-12 | 2020-06-02 | 华道(上海)生物医药有限公司 | Totally enclosed cell culture method |
| CN109913410A (en) * | 2019-04-19 | 2019-06-21 | 华子昂 | The emulation cultural method of stem cell |
| WO2021114129A1 (en) * | 2019-12-11 | 2021-06-17 | 中国科学院深圳先进技术研究院 | In vitro experimental model for research of mechanism of tumor perineural invasion |
| CN112940933A (en) * | 2021-01-29 | 2021-06-11 | 上海睿钰生物科技有限公司 | In-vitro life-sustaining perfusion culture system and control method thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN106467892B (en) | 2018-12-18 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN106467892A (en) | A kind of automatic cytological amplification system | |
| CN106635790B (en) | A kind of fully enclosed automatic cell culture system | |
| CN106479889B (en) | A kind of cell culture circulatory system | |
| CN204569936U (en) | A kind of small-sized fermentation tank group system | |
| CN206783688U (en) | A kind of multilevel cell amplification system | |
| CN107904169B (en) | Modularized bioreactor suitable for culturing multiple cell types | |
| CN106497783B (en) | A kind of dynamic circulation cell culture apparatus and cell culture processes | |
| JP2018529367A (en) | Bioreactor capable of shutting off gas supply | |
| CN206014949U (en) | A kind of nutrition liquid supplying device for cell culture | |
| CN106497784B (en) | A kind of control pressurer system and its compress control method for realizing culture solution and waste liquid exchange | |
| CN101748054B (en) | Photobioreactor for cultivating microalgae | |
| CN102108333B (en) | Fluidized bed bioreactor | |
| CN100497583C (en) | Safety high-efficient continuous enclosed type cell culture and virus production-inactivation system | |
| CN109825436A (en) | Totally-enclosed cell culture system | |
| CN103031251B (en) | Multifunctional plant excised root culture bioreactor system | |
| CN105255731A (en) | Circulation filling type cell culture system and bioreactor of circulation filling type cell culture system | |
| CN204162717U (en) | A kind of microorganism continuous culture device | |
| CN206887129U (en) | Disposable pneumo lift-type cell culture reactor | |
| CN107557299B (en) | Liquid atomization system for cell culture | |
| CN110129199A (en) | A kind of three-dimensional artificial culture systems of stem cell | |
| CN206014948U (en) | A kind of detecting system of cell cycle incubation | |
| CN203625382U (en) | Oscillating large-scale suspension cell culture device | |
| WO2020181709A1 (en) | Fully enclosed cell incubation system | |
| CN204848881U (en) | Simple and easy airlift bioreactor is used in teaching | |
| CN206843447U (en) | A kind of gravity flow photosynthetic reaction device |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |