CN107904167B - Automatic cell expansion culture equipment - Google Patents
Automatic cell expansion culture equipment Download PDFInfo
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- CN107904167B CN107904167B CN201711132497.2A CN201711132497A CN107904167B CN 107904167 B CN107904167 B CN 107904167B CN 201711132497 A CN201711132497 A CN 201711132497A CN 107904167 B CN107904167 B CN 107904167B
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/08—Flask, bottle or test tube
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
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- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/14—Pressurized fluid
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
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- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
- C12M41/34—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of gas
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/48—Automatic or computerized control
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Abstract
The invention relates to automatic cell expansion culture equipment, which aims to realize automatic liquid exchange in the cell culture process. The invention comprises the following steps: the cell culture box, set up support device, the controller in the cell culture box, be equipped with the control button on the shell of cell culture box, set up cell culture container, stock solution container, the cell culture liquid driving pump of connection on infusion bottle and bacterium blake bottle transfer line on the support device cell culture liquid driving pump is connected with the controller electricity, cell culture liquid driving pump passes through the controller control, wherein, support device includes the bottom plate, can dismantle the setting and be in cut apart the riser on the bottom plate, cut apart the riser and separate the cell culture box into left and right two parts, cut apart install on the riser and be used for supporting cell culture container support piece of cell culture container, be used for supporting stock solution container support piece and be used for supporting cell culture liquid driving pump's cell culture liquid driving pump support piece.
Description
Technical Field
The invention relates to automatic cell expansion culture equipment.
Background
Cell culture is an indispensable process in bioengineering technology that can be largely cultured from one cell into a simple single cell or a few differentiated multicellular. A large number of cells or their metabolites can be obtained by cell culture. Since most biological products are derived from cells, cell culture technology is the most central, basic technology in biotechnology. Cell culture work is now widely used in various fields of biology, medicine, new drug development, etc., and plays a vital role in the research of the whole life science.
Based on the foregoing, there is a need for a large automated cell culture system and a dedicated cell culture system for in vitro culturing of cell samples of different origins. The designer actively researches and innovates to create an automatic cell expansion culture device, so that the device has more industrial utilization value.
Disclosure of Invention
In order to solve the technical problems, the invention aims to provide the automatic cell expansion culture equipment which is reasonable in structure, convenient to use and capable of being controlled remotely.
The automatic cell expansion culture equipment of the invention comprises: the cell culture box comprises a bracket device and a controller, wherein the bracket device and the controller are arranged in the cell culture box, a control key is arranged on a shell of the cell culture box, a cell culture container, a liquid storage container and a cell culture liquid driving pump which are arranged on the bracket device are electrically connected with the controller, the cell culture liquid driving pump is controlled by the controller, the bracket device comprises a bottom plate and a partition vertical plate which is detachably arranged on the bottom plate, the partition vertical plate divides the cell culture box into a left part and a right part or a front part and a rear part, and a cell culture container support piece for supporting the cell culture container, a liquid storage container support piece for supporting the liquid storage container and a cell culture liquid driving pump support piece for supporting the cell culture liquid driving pump are arranged on the partition vertical plate;
the controller is also provided with a wireless communication module and is connected with a data interaction expansion interface, the controller is in data communication with the mobile terminal through the wireless communication module, the controller obtains the current cell culture environment in the incubator through a culture environment sensor arranged in the incubator, the current cell culture environment is output to the mobile terminal through the wireless communication module, and the mobile terminal outputs a control instruction to the controller through the wireless communication module, wherein the culture environment sensor at least comprises a temperature sensor, a humidity sensor and a carbon dioxide concentration sensor.
Further, cell culture container support piece, infusion bottle support piece constitute by extension board and movable orifice plate, the extension board is installed cut apart on the riser, movable orifice plate with the extension board passes through the loose-leaf connection, wherein be equipped with on the extension board of cell culture container support piece and vibrate the motor, vibrate the power take off end of motor with movable orifice plate contact, movable orifice plate through vibrate the drive of motor in order to realize movable orifice plate ration fluctuation.
Further, the rack device is provided in the cell incubator by two pairs of roller cross rail pairs, each roller cross rail pair comprising: the cell box comprises a lower guide rail arranged on the side wall of the cell box and an upper guide rail arranged on a bottom plate, wherein a roller retainer is arranged on the lower guide rail.
Further, a display screen and keys connected with the controller are arranged on the incubator shell.
Further, a culture bottle fixing seat is arranged on the movable pore plate, and the shape of the culture bottle fixing seat is matched with the shape of the contact surface of the movable pore plate when the culture bottle is placed on the movable pore plate so as to fix the culture bottle.
Further, the cell culture container comprises a transfection bottle and a plurality of amplification bottles, wherein each transfection bottle is provided with two cell culture liquid driving pumps respectively, one pump is responsible for adding the cell culture liquid for transfection in the process of transformation culture of cells, and the other pump is responsible for removing the cell culture liquid for transfection before amplification culture; two cell culture liquid driving pumps are respectively arranged in each amplification bottle, one pump is responsible for amplifying inoculated cells in a cell culture container, and the other pump is responsible for adding culture liquid into the amplification bottles during cell amplification culture.
Further, the cell culture fluid driven pump is a peristaltic pump or a syringe pump.
Further, the infusion tube is a silica gel tube with an inner diameter of 2mm and a wall thickness of 1mm
By means of the scheme, the automatic cell expansion culture equipment has at least the following advantages:
the support structure of the invention has reasonable design, realizes fixed-distance fluctuation of the movable pore plate through the oscillating motor, and achieves the aim of simulating manual shaking of the cell culture container and uniform cell suspension. The movable guide rail is arranged in the incubator for convenient use, so that the bracket device can conveniently enter and exit.
The invention realizes remote data interaction between the mobile terminal and the controller through the remote communication module.
The foregoing description is only an overview of the present invention, and is intended to provide a better understanding of the present invention, as it is embodied in the following description, with reference to the preferred embodiments of the present invention and the accompanying drawings.
Drawings
FIG. 1 is a schematic diagram of an automatic cell expansion culture apparatus according to the present invention;
FIG. 2 is a schematic view showing the structure of a cell culture vessel support of the automatic cell expansion culture apparatus of the present invention;
FIG. 3 is a schematic diagram showing vibration of a cell culture vessel support of the cell automatic expansion culture device of the present invention;
FIG. 4 is a schematic view of a reservoir support of the cell automatic expansion culture device of the present invention.
Detailed Description
The following describes in further detail the embodiments of the present invention with reference to the drawings and examples. The following examples are illustrative of the invention and are not intended to limit the scope of the invention.
Example 1
As shown in fig. 1, the cell automatic expansion culture apparatus of the present embodiment includes: cell culture case 1, set up support device, the controller in the cell culture case, be equipped with control button 2 on the shell of cell culture case, set up cell culture container, stock solution container, the cell culture liquid drive pump of connection on infusion bottle and bacterium blake bottle transfer line on the support device cell culture liquid drive pump is connected with the controller electricity, cell culture liquid drive pump passes through the controller control, wherein, support device includes bottom plate 3, can dismantle the setting and cut apart the riser on the bottom plate, cut apart the riser with cell culture incasement and separate into left and right two parts, cut apart and install the cell culture container support piece that is used for supporting cell culture container on the riser, be used for supporting the stock solution container support piece and be used for supporting cell culture liquid drive pump's cell culture liquid drive pump support piece.
The controller is also provided with a wireless communication module and is connected with a data interaction expansion interface, the controller is in data communication with the mobile terminal through the wireless communication module, the controller obtains the current cell culture environment in the incubator through a culture environment sensor arranged in the incubator, the current cell culture environment is output to the mobile terminal through the wireless communication module, and the mobile terminal outputs a control instruction to the controller through the wireless communication module, wherein the culture environment sensor at least comprises a temperature sensor, a humidity sensor and a carbon dioxide concentration sensor.
In this embodiment, cell culture container support piece, infusion bottle support piece constitute by extension board and movable orifice plate, the extension board is installed cut apart on the riser, movable orifice plate with the extension board passes through the loose-leaf connection, wherein be equipped with on cell culture container support piece's the extension board 28 and vibrate motor 27, vibrate motor's power take off end with movable orifice plate 29 contact, movable orifice plate pass through vibration motor's drive in order to realize movable orifice plate ration fluctuation.
In this embodiment, the front region is a transfection culture region for transforming cells, and is provided with two cell culture liquid driving pumps, one pump is responsible for adding the cell culture liquid for transfection in the transformation culture process, and the other pump is responsible for removing the cell culture liquid for transfection before amplification culture. The back region is an expansion culture region, and is intended to comprise 3 culture bottles, wherein each culture bottle is provided with 2 reagent delivery pumps, one pump is responsible for expanding inoculated cells into a cell culture container, and the other pump is responsible for adding culture solution into the cell culture container during cell expansion culture.
In this embodiment, a display screen and a key connected to the controller are provided on the incubator housing. And realizing remote data interaction between the mobile terminal and the controller through the remote communication module.
Example 2
On the basis of embodiment 1, in order to facilitate the support device to enter and exit the cell incubator, the support device is disposed in the cell incubator through two roller cross guide pairs, and each roller cross guide pair comprises: the cell box comprises a lower guide rail arranged on the side wall of the cell box and an upper guide rail arranged on a bottom plate, wherein a roller retainer is arranged on the lower guide rail.
Example 3
As shown in fig. 2, in the automatic cell expansion culture apparatus of this embodiment, on the basis of embodiment 1, in order to avoid dropping the movable orifice plate of the culture flask during the vibration process, a culture flask fixing seat is provided on the movable orifice plate, and the shape of the culture flask fixing seat 26 is adapted to the shape of the contact surface with the movable orifice plate when the culture flask is placed on the movable orifice plate so as to fix the culture flask.
In each of the above embodiments, the cell culture fluid driving pump is a peristaltic pump.
In each embodiment, the infusion tube is a silicone tube with an inner diameter of 2mm and a wall thickness of 1 mm.
The above description is only of the preferred embodiments of the present invention and is not intended to limit the present invention, and it should be noted that it is possible for those skilled in the art to make several improvements and modifications without departing from the technical principle of the present invention, and these improvements and modifications should also be regarded as the protection scope of the present invention.
Claims (3)
1. An automatic cell expansion culture apparatus, comprising: the cell culture box comprises a bracket device and a controller, wherein the bracket device and the controller are arranged in the cell culture box, a control key is arranged on a shell of the cell culture box, a cell culture container, a liquid storage container and a cell culture liquid driving pump which are arranged on the bracket device are electrically connected with the controller, the cell culture liquid driving pump is controlled by the controller, the bracket device comprises a bottom plate and a partition vertical plate which is detachably arranged on the bottom plate, the partition vertical plate divides the cell culture box into a left part and a right part or a front part and a rear part, and a cell culture container support piece for supporting the cell culture container, a liquid storage container support piece for supporting the liquid storage container and a cell culture liquid driving pump support piece for supporting the cell culture liquid driving pump are arranged on the partition vertical plate;
the controller is also provided with a wireless communication module, the controller is connected with a data interaction expansion interface, the controller performs data communication with the mobile terminal through the wireless communication module, the controller obtains the current cell culture environment in the incubator through a culture environment sensor arranged in the incubator, the current cell culture environment is output to the mobile terminal through the wireless communication module, and the mobile terminal outputs a control instruction to the controller through the wireless communication module, wherein the culture environment sensor at least comprises a temperature sensor, a humidity sensor and a carbon dioxide concentration sensor;
the cell culture container support and the transfusion bottle support are composed of a support plate and a movable pore plate, the support plate is arranged on the partition vertical plate, the movable pore plate is connected with the support plate through a hinge, the support plate of the cell culture container support is provided with an oscillating motor, the power output end of the oscillating motor is in contact with the movable pore plate, and the movable pore plate is driven by the oscillating motor to quantitatively undulate;
the bracket device is arranged in the cell incubator through two roller cross guide pairs, and each roller cross guide pair comprises: a lower guide rail arranged on the side wall of the cell box and an upper guide rail arranged on the bottom plate, wherein a roller retainer is arranged on the lower guide rail;
the incubator shell is provided with a display screen and keys connected with the controller;
the movable pore plate is provided with a culture bottle fixing seat, and the shape of the culture bottle fixing seat is matched with the shape of the contact surface of the movable pore plate when the culture bottle is placed on the movable pore plate so as to fix the culture bottle;
the cell culture container comprises a transfection bottle and a plurality of amplification bottles, wherein each transfection bottle is provided with two cell culture liquid driving pumps respectively, one pump is responsible for adding cell culture liquid for transfection in the process of transformation and culture of cells, and the other pump is responsible for removing the cell culture liquid for transfection before amplification and culture; two cell culture liquid driving pumps are respectively arranged in each amplification bottle, one pump is responsible for amplifying inoculated cells in a cell culture container, and the other pump is responsible for adding culture liquid into the amplification bottles during cell amplification culture.
2. The cell automatic expansion culture apparatus of claim 1, wherein the cell culture fluid driven pump is a peristaltic pump or a syringe pump.
3. The automatic cell expansion and culture apparatus according to claim 1, wherein the transfusion tube is a silicone tube having an inner diameter of 2mm and a wall thickness of 1 mm.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711132497.2A CN107904167B (en) | 2017-11-15 | 2017-11-15 | Automatic cell expansion culture equipment |
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| CN201711132497.2A CN107904167B (en) | 2017-11-15 | 2017-11-15 | Automatic cell expansion culture equipment |
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| CN107904167A CN107904167A (en) | 2018-04-13 |
| CN107904167B true CN107904167B (en) | 2023-06-06 |
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| WO2015165583A1 (en) * | 2014-04-28 | 2015-11-05 | Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. | Fermentation systems |
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| CN201990683U (en) * | 2011-01-06 | 2011-09-28 | 山东大学 | Isolated culture cell anoxic instrument |
| CN104302757A (en) * | 2012-03-15 | 2015-01-21 | 赛普罗塞拉公司 | Automated device and automated process for cell culture |
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