CN208346188U - It is able to achieve energy ground-wire monitoring and the cell culture system remotely monitored in real time - Google Patents
It is able to achieve energy ground-wire monitoring and the cell culture system remotely monitored in real time Download PDFInfo
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- CN208346188U CN208346188U CN201721523571.9U CN201721523571U CN208346188U CN 208346188 U CN208346188 U CN 208346188U CN 201721523571 U CN201721523571 U CN 201721523571U CN 208346188 U CN208346188 U CN 208346188U
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Abstract
The utility model relates to it is a kind of be able to achieve can ground-wire monitoring and the remotely cell culture system that monitors in real time, in order to realize long-range control, monitor cell culture and design.The utility model includes: the remote control terminal that the controller of cell incubator and cell incubator is connected by wired or wireless way, Tissue Culture Flask is equipped in cell incubator, liquid storage bottle, component is arranged in environment for being adjusted to the environmental parameter in cell incubator, for acquiring the image collecting device of Tissue Culture Flask inner cell upgrowth situation, Tissue Culture Flask be connected to liquid storage bottle by several perfusion tubes, cell culture fluid transfer tube is respectively equipped on perfusion tube, environment be arranged component, cell culture fluid transfer tube, the controller that image collecting device is separately connected, each culture bottle respectively corresponds an image collecting device, the shell of cell incubator is equipped with display screen and key connected to the controller for showing cell growth condition.
Description
Technical field
The utility model relates to a kind of cell culture systems for being able to achieve the real-time ground-wire monitoring of energy and remotely monitor.
Background technique
Cell culture is an essential process in biotechnology, which can be by a cell through excessive
Amount culture becomes many cells that are simple unicellular or seldom breaking up.Pass through the available a large amount of cell of cell culture or its generation
Thank to product.Since most of biological products are got from cell, cell culture technology is most crucial, most in biotechnology
The technology on basis.Cell culture work has been widely used in the every field such as biology, medicine, new drug development, it is to entire
Life science plays the role of vital.
Currently, European and American developed countries have carried out the R&D work of pertinent instruments device one after another, cell culture system device is
Start that commercialized development is done step-by-step.According to the difference of research purpose, large automatic cell culture system and dedicated can be used
Cell culture system carries out in vitro culture to the cell sample of separate sources.In external Related product, in the automatic filling of culture solution
It does not fully achieve automation on stream also, is deposited on image real-time monitoring, parameter monitoring and the different types of cell sample of processing yet
In some shortcomings.So far, in the exploitation of cell culture system, the Related product that the country not yet develops independent research is set
It is standby, changing liquid automatically and on-line monitoring are carried out especially for the cell sample (attached cell and suspension cell) of different growth patterns
Cell culture system.
In view of above-mentioned defect, the designer is actively subject to research and innovation, is able to achieve the real-time ground wire of energy to found one kind
Monitoring and the cell culture system remotely monitored, make it with more the utility value in industry.
Utility model content
In order to solve the above technical problems, the purpose of the utility model is to provide one kind can record incubator inner cell in real time
Production status and the cell culture system for being able to achieve the real-time ground-wire monitoring of energy and remotely monitor for realizing automatic perfusion.
The utility model is able to achieve energy ground-wire monitoring and the cell culture system remotely monitored in real time, comprising: cell culture
The remote control terminal that case is connect with the controller of the cell incubator by wired or wireless way, the cell incubator
It is interior that equipped with Tissue Culture Flask, liquid storage bottle, the environment for being adjusted to the environmental parameter in cell incubator, component is set, it uses
Pass through in image collecting device, the Tissue Culture Flask and the liquid storage bottle of acquisition Tissue Culture Flask inner cell upgrowth situation several
Perfusion tube connection, is respectively equipped with cell culture fluid transfer tube on the perfusion tube, and component, cell culture is arranged in the environment
The controller that liquid transfer tube, image collecting device are separately connected, each culture bottle respectively corresponds an image collecting device, described thin
The shell of born of the same parents' incubator is equipped with the key for showing the display screen of cell growth condition and connecting with the controller.
Further, described image acquisition device includes focusing electric platforms, is arranged on the focusing electric platforms
Photomoduel, objective lens unit and laser ranging system before the photomoduel camera lens is set；Wherein, in image collector
When setting acquisition culture bottle image, distance of the photomoduel apart from culture bottle is measured by laser ranging system first, is then passed through
Focusing electric platforms focus, the image of last image acquisition device culture bottle, and export to controller.
Further, the object lens of described image acquisition device are replaceable, and camera lens can match × 4, × 10, × 20, × 40, ×
60 5 enlargement ratios.
Further, holder device is equipped in the cell incubator, the holder device includes bottom plate, is arranged described
Center vertical plate on bottom plate, the center vertical plate will be divided into left and right two parts, pacify on the center vertical plate in cell incubator
Equipped be used to support the Tissue Culture Flask supporting element of Tissue Culture Flask, the liquid storage bottle supporting element for being used to support liquid storage bottle, for branch
It supports the Image Acquisition supporting element of described image acquisition device and is used to support the cell culture fluid drive of cell culture fluid transfer tube
Dynamic pump supporting element.
Further, the Tissue Culture Flask supporting element, infusion bottle supporting element are formed by by supporting plate and removable orifice plate,
The supporting plate is mounted on the center vertical plate, and the removable orifice plate is connected with the supporting plate by loose-leaf, wherein cell culture
The supporting plate of bottle supporting element is equipped with concussion motor, and the power output end of the concussion motor is contacted with the removable orifice plate, institute
The removable orifice plate stated is by the driving of concussion motor to realize that removable orifice plate quantitatively rises and falls.
Further, the holder device is arranged in the cell incubator by two secondary cross-roller guide pairs, respectively
Cross-roller guide pair includes: the lower guideway for being installed on cell case side wall and the upper rail on the bottom plate being installed on, and is led under described
Rail is equipped with roller retainer.
Further, the removable orifice plate is equipped with culture bottle fixing seat, the shape and culture of the culture bottle fixing seat
Bottle is adapted with the shape of removable orifice plate contact surface when being placed in removable orifice plate with the fixation culture bottle.
Further, the Tissue Culture Flask includes transfection bottle, and several amplification bottles, each bottle that transfects is with two cell trainings respectively
Nutrient solution transfer tube, a responsible cell add transfection cell culture fluid in transformation incubation, and another pumps responsible amplification
Transfection cell culture fluid is removed before culture；Two cell culture fluid transfer tubes are respectively configured in each amplification bottle, and one is responsible for thin
Inoculating cell is expanded in born of the same parents' culture bottle, another pump is responsible for adding culture solution into amplification bottle during cell amplification cultivation.
Further, the cell culture fluid transfer tube is peristaltic pump.
Further, it is 2mm that the perfusion tube, which is internal diameter, and wall thickness is the silicone tube of 1mm.
According to the above aspect of the present invention, the utility model is able to achieve the real-time ground-wire monitoring of energy and the cell culture system remotely monitored extremely
It has the advantage that less
The utility model can obtain the growth shape of cell by the way that image collecting device is arranged in cell incubator in real time
Condition, and the upgrowth situation of cell is exported to terminal or remote display terminals are locally displayed, so that user understands cell in real time
Growth conditions.
Unlatching or pass of the utility model according to the real-time each cell culture fluid transfer tube of upgrowth situation image control
It closes, to realize the automatic liquid-feeding control of culture bottle.
The utility model must focus before cell sample acquisition image since culture bottle requires periodically oscillation.It adjusts
Focal length is realized by focusing electric platforms, image collecting device and objective lens unit is mounted on focusing electric platforms, according to need
The direction of motion and move distance of focusing electric platforms are controlled, realizes focus adjustment.
The utility model cell culture can automatically realize that overall process is totally-enclosed with passage amplification, greatly reduce thin
Born of the same parents cultivate the pollution risk faced；Without personnel's auxiliary operation, the passage requirement of attached cell and suspension cell is adapted to；Cell
Cultivate overall process equipment working condition parameter can real-time display, cell growth status image information can real time inspection；The long-range prison of configuration
Control system can realize equipment telemonitoring and control.
The above description is merely an outline of the technical solution of the present invention, in order to better understand the skill of the utility model
Art means, and can be implemented in accordance with the contents of the specification, below on the preferred embodiment of the present invention and the accompanying drawings in detail
It describes in detail bright as after.
Detailed description of the invention
Fig. 1 is the schematic diagram that the utility model is able to achieve energy real-time ground-wire monitoring and the cell culture system remotely monitored；
Fig. 2 is the cell culture that the utility model is able to achieve energy real-time ground-wire monitoring and the cell culture system remotely monitored
The schematic diagram of case；
Fig. 3 is the internal stent that the utility model is able to achieve energy real-time ground-wire monitoring and the cell culture system remotely monitored
Schematic diagram；
Fig. 4 is the culture bottle branch that the utility model is able to achieve energy real-time ground-wire monitoring and the cell culture system remotely monitored
Support member schematic diagram；
Fig. 5 is the culture bottle branch that the utility model is able to achieve energy real-time ground-wire monitoring and the cell culture system remotely monitored
The concussion schematic diagram of support member；
Fig. 6 is the liquid storage bottle branch that the utility model is able to achieve energy real-time ground-wire monitoring and the cell culture system remotely monitored
The schematic diagram of support member；
Fig. 7 is the internal stent that the utility model is able to achieve energy real-time ground-wire monitoring and the cell culture system remotely monitored
Schematic diagram；
Fig. 8 is that the utility model is able to achieve energy ground-wire monitoring and the image collecting device schematic diagram remotely monitored in real time.
Specific embodiment
With reference to the accompanying drawings and examples, specific embodiment of the present utility model is described in further detail.Below
Embodiment is not intended to limit the scope of the present invention for illustrating the utility model.
Embodiment 1
As shown in Figure 1, the present embodiment is able to achieve energy ground-wire monitoring and the cell culture system remotely monitored in real time, comprising:
The remote control terminal 01 that cell incubator is connect with the controller of the cell incubator by wired or wireless way, it is described
Tissue Culture Flask, liquid storage bottle, the environment for being adjusted to the environmental parameter in cell incubator are equipped in cell incubator 1
Component is set, for acquiring the image collecting device, the Tissue Culture Flask and storage of Tissue Culture Flask inner cell upgrowth situation
Liquid bottle is connected to by several perfusion tubes, and cell culture fluid transfer tube, the environment setting are respectively equipped on the perfusion tube
The controller that component, cell culture fluid transfer tube, image collecting device are separately connected, each culture bottle respectively correspond an image and adopt
Acquisition means, the shell of the cell incubator be equipped with display screen for showing cell growth condition and with the controller
The key 11 of connection；
The control instruction control environment setting component that the controller is exported according to remote control terminal or local key
Working condition.The unlatching of the infusion pump is closed, and control described image acquisition device working condition；
Described image acquisition device for obtaining the real-time upgrowth situation image of each culture bottle inner cell, and exports the reality
When upgrowth situation image to controller, the controller exports output institute according to the request of remote terminal or with predetermined time interval
Real-time upgrowth situation image is stated to remote terminal, the controller is requested according to user key-press or exported with predetermined time interval defeated
The real-time upgrowth situation image to local display is shown out；
The controller acquires cell incubator current operating environment parameter with predetermined time interval, and exports the current work
Make environmental parameter to local display and remote terminal；
The remote terminal is according to the real-time upgrowth situation image of cell and cell incubator current operating environment of acquisition
Parameter exports corresponding control instruction to controller, and the controller is arranged according to the control instruction control environment
The working condition of component, infusion pump, image collecting device.
The present embodiment Mobile phone with function of remote monitoring is mainly made of 4G cell phone and APP, and 4G cell phone monitors mobile phone as device far end
Version software operation carrier；APP is responsible for real-time display equipment working condition parameter, cell culture environment parameter, cytological map on mobile phone
Information and the equipment key operation remote controls such as picture.
Local device is cultivated automatically by cell culture automatic perfusion system, the automatic culture environment control system of cell, cell
Effect monitoring system and cell are cultivated control automatically and are formed with four part of display system.Cell culture automatic perfusion system is realized
Cell based on perfusion mode is cultivated automatically；The automatic culture environment control system of cell realize in perfusion mode reagent storage and
The environmental conditions such as temperature, the gas concentration lwevel of cell cultivation requirement；The automatic culture effect monitoring system of cell is quasi- with cytological map
As being foundation, realizes and the cell sample culture effect cultivated automatically is monitored；Cell is cultivated control automatically and is intended with display system
Above system it is integrated with merge on the basis of, local monitor of the realization to equipment.
Embodiment 2
The present embodiment is able to achieve energy ground-wire monitoring and the cell culture system remotely monitored in real time, on the basis of embodiment 1
On, described image acquisition device includes focusing electric platforms 002, the photomoduel 003 on the focusing electric platforms is set,
Objective lens unit 004 and laser ranging system 001 before the photomoduel camera lens is set.
In the present embodiment, the quasi- automatic culture effect monitoring system of cell is mainly hand by cell sample image real time monitoring
Section, the cell sample growing state that whole process record is cultivated automatically assess cell sample state for experimenter and provide foundation.Originally it sets
Intend 4 sets of image recorders of configuration in standby, form image recorder array, records transfecting cultured cells culture bottle and 3 respectively
A amplification cultivation bottle cell growth status.
It is quasi- by photomoduel, focusing platform, objective lens unit and laser ranging module that cell sample image monitors component in real time
Composition.Object lens are replaceable, and camera lens can match × 4, × 10, × 20, × 40, × 60 equal five enlargement ratios.Due to cell culture
Bottle requires periodically oscillation, must focus before cell sample acquisition image.It focuses and intends realizing by electric platforms, it will
Cmos component and objective lens unit be mounted on focusing electric platforms on, as needed control the electric platforms direction of motion and movement away from
From realization focus adjustment.
Cell cultivates working environment of the control with display system by control incubator automatically, and experimental cell is cultivated automatically,
And above-mentioned parameter and cell growth process transmission of video images to equipment local terminal and remote terminal, user can be slapped accordingly
Hold cells grown environmental parameter and situation.Control system can respond the control of user at the terminal, realize the long-range of breeding apparatus
Control.
Embodiment 3
The present embodiment is able to achieve energy ground-wire monitoring and the cell culture system remotely monitored in real time, on the basis of embodiment 1
On, holder device is equipped in the cell incubator, the holder device includes bottom plate 24, and the center on the bottom plate is arranged in
Vertical plate, the center vertical plate will be divided into left and right two parts, be equipped with and be used to support on the center vertical plate in cell incubator
The Tissue Culture Flask supporting element of Tissue Culture Flask, is used to support described image at the liquid storage bottle supporting element 23 for being used to support liquid storage bottle
The Image Acquisition supporting element of acquisition device and the cell culture fluid transfer tube supporting element for being used to support cell culture fluid transfer tube.
In the present embodiment, the Tissue Culture Flask supporting element, infusion bottle supporting element are made of supporting plate and removable orifice plate,
The supporting plate is mounted on the center vertical plate, and the removable orifice plate is connected with the supporting plate by loose-leaf, wherein cell culture
The supporting plate of bottle supporting element is equipped with concussion motor, and the power output end of the concussion motor is contacted with the removable orifice plate, institute
The removable orifice plate stated is by the driving of concussion motor to realize that removable orifice plate quantitatively rises and falls.
In the present embodiment, cell incubator is responsible for cell culture environment foundation, and center bearing bracket is used to support cell culture system
System monitors system with the automatic culture effect of cell.Movable guiding rail is used between cell incubator and center bearing bracket, facilitates center bearing bracket
Pass in and out incubator.
The function of center bearing bracket is to provide support for other systems, mainly by intermediate vertical plate, culture bottle support, backup bottle
The parts such as support, the support of wriggling modular support, image capturing system 22 and reagent bottle support form.
Culture bottle support is made of removable orifice plate, vibration motor 27, supporting plate 28, culture bottle fixing seat and flap, movable span
Coupled between plate 29 and intermediate vertical plate by flap, it is ensured that orifice plate can be freely rotated.Supporting plate is fixed on intermediate vertical plate corresponding position,
Vibration motor is installed on supporting plate.
Vibration motor is coupled by movable part with orifice plate, realizes that two pieces of orifice plate spacings rise and fall, and is reached simulation and is shaken manually
Tissue Culture Flask, the target of uniform cell suspension.
Peristaltic pump support provides installation interface for cell culture system, is mainly made of mounting plate and level board.Peristaltic pump
Array 21 is installed on level board, and mounting plate is realized to be coupled with intermediate vertical plate.
The quasi- automatic culture effect monitoring system of cell is mainly means, whole process record by cell sample image real time monitoring
Automatically the cell sample growing state cultivated assesses cell sample state for experimenter and provides foundation.Intend configuration 4 in this equipment
Image recorder is covered, image recorder array is formed, records transfecting cultured cells culture bottle and 3 amplification cultivation bottles respectively
Cell growth status.
Embodiment 4
The present embodiment is able to achieve energy ground-wire monitoring and the cell culture system remotely monitored in real time, the Tissue Culture Flask packet
Transfection bottle is included, several amplification bottles divide proparea and back zone two parts, and proparea is the transfection cultivation region of engineered cells, match two cells
Culture solution transfer tube, a responsible cell add transfection cell culture fluid in transformation incubation, and another pumps responsible expansion
Transfection cell culture fluid is removed before increasing culture.Back zone is amplification cultivation area, intends including 3 culture bottles, each culture bottle configuration 2
Platform reagent transfer pump, a pump are responsible for expanding inoculating cell into Tissue Culture Flask, and another pump is responsible in cell amplification cultivation
Period adds culture solution into Tissue Culture Flask.
The function of the automatic culture environment control system of cell is to control cell culture demand under the regulation of general control system
Environmental condition, from the temperature in main regulation cell culture area maintain 36.5 DEG C of ± 0.5 DEG C of ranges, humidity be greater than 85%, CO2 it is dense
Degree realizes that cell culture area sterilizes in 5% ± 0.5% range, and according to experiment demand automatically.
Embodiment 5
The present embodiment is able to achieve energy ground-wire monitoring and the cell culture system remotely monitored in real time, on the basis of embodiment 3
On, the removable orifice plate is equipped with culture bottle fixing seat, and the shape and culture bottle of the culture bottle fixing seat are placed on movable span
It is adapted when on plate with the shape of removable orifice plate contact surface with the fixation culture bottle.
In the various embodiments described above, in order to facilitate holder device into and out of cell incubator, the holder device passes through two secondary rollings
Column cross slideway pair is arranged in the cell incubator, and each cross-roller guide pair includes: to be installed under cell case side wall
Upper rail on guide rail and the bottom plate being installed on, the lower guideway are equipped with roller retainer.
In the various embodiments described above, ultraviolet radiator, dioxide bottle, carbon dioxide sensor are equipped in the incubator,
The ultraviolet radiator, dioxide bottle, carbon dioxide sensor are connect with the controller respectively.
In the various embodiments described above, the basic function of controller requires as follows:
(1) temperature and gas concentration lwevel setting of cell incubator work are realized；
(2) ultraviolet lamp time switch in cell incubator is controlled；
(3) image capturing system controlled in cell incubator carries out image cell collection as required, including headlamp is opened
It opens, close control；Image capture device starting stops control；Image Acquisition, transmission and storage etc.；
(4) cell culture passages process is controlled, the job order not less than 12 multi-channel peristaltic pumps, work can be independently arranged
Make the running parameters such as time and operating time, and the above-mentioned peristaltic pump of independent control；It can independent control 2 disturbance linear motor works
Make time and operating time, and the above-mentioned disturbance linear motor of independent control；
(5) experiment flow is controlled, can start, suspend, arrestment；
(6) control system can be in the case where local and remote terminal user controls, and environmental parameter and video information are passed through net
Network interface is transferred to above-mentioned terminal；
(7) controlling terminal shows equipment working state parameter, comprising: cell incubator temperature and humidity, gas concentration lwevel；It is purple
Outer lamp working condition and countdown working time；Equipment heated condition, inflated condition；Equipment is normal, abnormal operation acousto-optic
Alarm indication；
(8) controlling terminal can set and display cell culture experiments information, comprising: Experiment name setting and display, Beijing
At the end of time, experiment time duration, experiment progress bar, cell sample micro-image, peristaltic pump quantity and working condition, experiment
Acousto-optic remind.
Utility model works mode are as follows: after acquiring enough T cells from the patient, cell is added together with culture solution
Enter into transfection Tissue Culture Flask, destination agent is added in reagent storage bottle.As required by the storage of pump, culture bottle and reagent
Bottle is connected, and checks whether each pipeline trend is correct.After setting cell culture parameter according to demand, by the starting of input interface
After key, equipment enters automatic operation mode, and equipment cell culture area temperature is 36.5 DEG C ± 0.5 DEG C, and gas concentration lwevel is
5% ± 0.5%, humidity is greater than 85%.It, can be real by local device display system or remote monitoring system in cell cultivation process
When monitoring with understand cell culture parameter, process and cell culture quality.After cell culture process, equipment will with sound,
The form of light prompts.
The above is only the preferred embodiment of the utility model, is not intended to limit the utility model, it is noted that
For those skilled in the art, without deviating from the technical principle of the utility model, it can also do
Several improvements and modifications out, these improvements and modifications also should be regarded as the protection scope of the utility model.
Claims (10)
1. a kind of be able to achieve energy ground-wire monitoring and the cell culture system remotely monitored in real time characterized by comprising cell training
Support the remote control terminal that case is connect with the controller of the cell incubator by wired or wireless way, the cell culture
Component is set equipped with Tissue Culture Flask, liquid storage bottle, the environment for being adjusted to the environmental parameter in cell incubator in case,
If the image collecting device for acquiring Tissue Culture Flask inner cell upgrowth situation, the Tissue Culture Flask and liquid storage bottle pass through
Dry perfusion tube connection is respectively equipped with cell culture fluid transfer tube, the environment setting component, cell training on the perfusion tube
The controller that nutrient solution transfer tube, image collecting device are separately connected, each culture bottle respectively corresponds an image collecting device, described
The shell of cell incubator is equipped with the key for showing the display screen of cell growth condition and connecting with the controller.
2. according to claim 1 be able to achieve energy ground-wire monitoring and the cell culture system remotely monitored in real time, feature
It is, described image acquisition device includes focusing electric platforms, and the photomoduel on the focusing electric platforms, setting is arranged in
Objective lens unit and laser ranging system before the photomoduel camera lens；Wherein, in image acquisition device culture bottle
When image, distance of the photomoduel apart from culture bottle is measured by laser ranging system first, then passes through focusing electric platforms
It focuses, the image of last image acquisition device culture bottle, and exports to controller.
3. according to claim 2 be able to achieve energy ground-wire monitoring and the cell culture system remotely monitored in real time, feature
It is, the object lens of described image acquisition device are replaceable, and camera lens can match × 4, × 10, × 20, × 40, × 60 five times magnifications
Rate.
4. according to claim 2 be able to achieve energy ground-wire monitoring and the cell culture system remotely monitored in real time, feature
It is, holder device is equipped in the cell incubator, the holder device includes bottom plate, and the center on the bottom plate is arranged in
Vertical plate, the center vertical plate will be divided into left and right two parts, be equipped with and be used to support on the center vertical plate in cell incubator
The Tissue Culture Flask supporting element of Tissue Culture Flask, the liquid storage bottle supporting element for being used to support liquid storage bottle are used to support described image and adopt
The Image Acquisition supporting element of acquisition means and the cell culture fluid transfer tube supporting element for being used to support cell culture fluid transfer tube.
5. according to claim 4 be able to achieve energy ground-wire monitoring and the cell culture system remotely monitored in real time, feature
It is, the Tissue Culture Flask supporting element, infusion bottle supporting element are made of supporting plate and removable orifice plate, and the supporting plate is mounted on
On the center vertical plate, the removable orifice plate is connected with the supporting plate by loose-leaf, wherein the supporting plate of Tissue Culture Flask supporting element
It is equipped with concussion motor, the power output end of the concussion motor is contacted with the removable orifice plate, and the removable orifice plate is logical
The driving of concussion motor is crossed to realize that removable orifice plate quantitatively rises and falls.
6. according to claim 4 be able to achieve energy ground-wire monitoring and the cell culture system remotely monitored in real time, feature
It is, the holder device is arranged in the cell incubator by two secondary cross-roller guide pairs, each cross-roller guide
Pair includes: the lower guideway for being installed on cell case side wall and the upper rail on the bottom plate being installed on, and the lower guideway is equipped with roller
Retainer.
7. according to claim 5 be able to achieve energy ground-wire monitoring and the cell culture system remotely monitored in real time, feature
It is, the removable orifice plate is equipped with culture bottle fixing seat, and the shape and culture bottle of the culture bottle fixing seat are placed on activity
It is adapted when on orifice plate with the shape of removable orifice plate contact surface with the fixation culture bottle.
8. according to claim 3 be able to achieve energy ground-wire monitoring and the cell culture system remotely monitored in real time, feature
It is, the Tissue Culture Flask includes transfection bottle, and several amplification bottles respectively transfect bottle and match two cell culture fluid transfer tubes respectively,
One responsible cell adds transfection cell culture fluid in transformation incubation, and another pump removes before being responsible for amplification cultivation
Cell culture fluid is used in transfection；Two cell culture fluid transfer tubes are respectively configured in each amplification bottle, and one is responsible for into Tissue Culture Flask
Inoculating cell is expanded, another pump is responsible for adding culture solution into amplification bottle during cell amplification cultivation.
9. according to claim 3 be able to achieve energy ground-wire monitoring and the cell culture system remotely monitored in real time, feature
It is, the cell culture fluid transfer tube is peristaltic pump.
10. according to claim 3 be able to achieve energy ground-wire monitoring and the cell culture system remotely monitored in real time, feature
It is, the perfusion tube is that internal diameter is 2mm, and wall thickness is the silicone tube of 1mm.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111592986A (en) * | 2020-05-27 | 2020-08-28 | 南京铭声通电子科技有限公司 | Intelligent culture and remote observation culture device and method |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111592986A (en) * | 2020-05-27 | 2020-08-28 | 南京铭声通电子科技有限公司 | Intelligent culture and remote observation culture device and method |
| CN111592986B (en) * | 2020-05-27 | 2024-03-19 | 南京兰伯艾克斯生物科技有限公司 | Intelligent culture and remote observation culture device and method |
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