CN212357270U

CN212357270U - Automatic IVF culture and time difference analysis device and matched culture dish

Info

Publication number: CN212357270U
Application number: CN202021166524.5U
Authority: CN
Inventors: 周汉宇; 李昕
Original assignee: Suzhou Meyuli Biotechnology Co ltd
Current assignee: Suzhou Fengtai Medical Technology Co ltd
Priority date: 2020-06-22
Filing date: 2020-06-22
Publication date: 2021-01-15
Anticipated expiration: 2030-06-22

Abstract

The utility model discloses an automatic change IVF and cultivate and time difference analytical equipment and supporting culture dish thereof, the device comprises main part device, supporting gas-liquid flow device and comprehensive controller, and main part device bears device and CO including thermal-insulated sealed cowling, application of sample device, culture dish₂Concentration, O₂Monitoring and adjusting devices for concentration, humidity and temperature, and the like; be equipped with oil blanket chamber recess, culture cell, culture dish culture solution application of sample hole and culture dish waste discharge hole on this culture dish, the below of oil blanket chamber recess is equipped with the culture solution cushion chamber with culture dish culture solution application of sample hole and culture cell application of sample hole intercommunication to and the waste liquid cushion chamber with culture dish waste discharge hole and culture cell waste discharge hole intercommunication. The utility model discloses can maintain the relative invariant of cultivating the environment, and spiral flow trades the liquid technique and can guarantee the clear away effect of sperm cell, cumulus cell, metabolic waste to realize that the concentration gradient between each culture solution changes, the maximize reduces the external stimulation to the embryonated egg, effectively improves fertility rate and blastocyst culture rate.

Description

Automatic IVF culture and time difference analysis device and matched culture dish

Technical Field

The utility model belongs to the biomedical equipment field, concretely relates to automatic IVF (also known as test tube baby) culture and time difference analytical equipment and supporting culture dish by computer or PLC control for automatic culture people or animal embryonated egg and real-time microscopic observation aassessment culture process.

Background

In recent years, the number of couples who need to test tube babies has increased dramatically due to the release of the national second birth policy, environmental influences, and the like, and among them, the number of couples who need to test a first generation of test tube babies is large (about 60%). In the IVF (also called tube baby) experiment process, each woman generally takes about ten ova to strip cumulus cells, make liquid drops, seal oil, add ova and sperm cells, culture and transplant, in the process, three to four culture solutions in different periods are required to be replaced according to the culture condition, and the liquid drops need to be made again when the culture solution is replaced every time.

At present, the tedious liquid drop making and the liquid changing work of the culture solution still need to be finished by manual operation, the external stimulation in the culture process is large, and statistics is carried out on the fertilization rate of 70% and the blastocyst culture rate of 40% of a first-generation test tube infant experiment, so that the development of the IVF industry is greatly limited. And the strict checking system of the IVF laboratory also limits the number of technicians in the IVF laboratory to a certain extent, which indirectly causes low efficiency and high cost of the IVF experiment.

In addition, for the receiverThe time difference analysis is carried out by combining the microscopic imaging morphological analysis technology in the sperm and egg culture process, and the fertilized egg is ensured to be in a relatively constant CO in the process₂Concentration, O₂Concentration, temperature, humidity and relative light-tight environment to reduce the stimulation of external factors to the embryonated egg, the current experimental apparatus is difficult to guarantee the environmental requirement to among the embryonated egg culture analysis process.

SUMMERY OF THE UTILITY MODEL

To the problem that above-mentioned prior art exists, the utility model provides an automatic change IVF and cultivate with time difference analytical equipment and supporting culture dish, can the maximize reduce external environment and to the stimulation of embryonated egg, improve fertility rate and blastocyst culture rate.

For solving the technical problem, realize above-mentioned technological effect, the utility model discloses a following technical scheme realizes:

an automatic IVF culture and time difference analysis device comprises a main body device, a matched gas-liquid flow device and a comprehensive controller;

the main body device comprises an openable and closable heat insulation sealing cover, and a sample adding device and a culture dish bearing device are arranged in the heat insulation sealing cover; CO is arranged on the inner wall of the heat insulation sealing cover₂Concentration electrochemical detector, CO₂Concentration monitoring light source, humidity monitoring detector, humidity monitoring light source, temperature sensing device and O₂Concentration electrochemical detector, and the CO₂Concentration electrochemical detector and said CO₂The concentration monitoring light source sets up in opposite directions, the humidity monitoring detector with the humidity monitoring light source sets up in opposite directions, it is equipped with spraying humidification device, exhaust apparatus, dehydrating unit, CO to inlay on the casing of heat-insulating sealing cover₂Air intake device and O₂Air inlet means, and the CO₂Air intake device and said O₂The air inlet end of the air inlet device is connected with the shell of the heat insulation sealing cover after passing through the air heating and filtering filling device;

the sample adding device is positioned on the upper half part in the heat insulation sealing cover and comprises a sample adding device fixing table, the sample adding device fixing table is connected with a sample adding device fixing table vertical moving motor arranged on the inner top surface of the heat insulation sealing cover through a sample adding device fixing table supporting column, and the sample adding device fixing table is vertically movably hung on the upper half part in the heat insulation sealing cover through the matching of the sample adding device fixing table supporting column and the sample adding device fixing table vertical moving motor; the upper surface of the sample adding device fixing table is provided with a first culture solution sample adding pump, a second culture solution sample adding pump, a third culture solution sample adding pump and a fourth culture solution sample adding pump, the lower surface of the sample adding device fixing table is provided with a culture solution sample adding device and a culture dish lighting light source, the lower ends of the first culture solution sample adding pump, the second culture solution sample adding pump, the third culture solution sample adding pump and the fourth culture solution sample adding pump are all connected with a culture solution sample adding needle of the sample adding device, and the lower end of the culture solution sample adding device is provided with a culture solution sample adding needle of the sample adding device; meanwhile, a sperm cell sample adding device, a waste discharging device and a paraffin oil sample adding device which can move vertically are respectively arranged on the sample adding device fixing table through a sperm cell sample adding device vertical moving motor, a waste discharging device vertical moving motor and a paraffin oil sample adding device vertical moving motor, and the lower ends of the sperm cell sample adding device, the waste discharging device and the paraffin oil sample adding device are respectively provided with a sperm cell sample adding device sample adding needle, a waste discharging device sample sucking needle and a paraffin oil sample adding device sample adding needle;

the culture dish bearing device is positioned at the lower half part in the heat insulation sealing cover, the culture dish bearing device comprises a round and transparent culture dish bearing platform, a culture dish bearing platform flange is arranged on the circumference of the culture dish bearing platform, a pair of culture dish clamping grooves for fixing a matched culture dish are arranged on the culture dish bearing platform flange, the bottom of the culture dish bearing platform is connected with a culture dish bearing platform rotating motor arranged at the inner bottom surface of the heat insulation sealing cover through a culture dish bearing platform supporting column, the culture dish bearing platform is horizontally and rotatably arranged at the lower half part in the heat insulation sealing cover through the matching of the culture dish bearing platform rotating motor and the culture dish bearing platform supporting column, a micro camera supporting column and a micro camera platform are sequentially sleeved on the culture dish bearing platform supporting column, and the upper end of the micro camera platform supporting column is connected with the micro camera platform, the lower end of the supporting column of the microscope camera carrying platform is connected with a rotation motor of the microscope camera carrying platform, the rotation motor of the microscope camera carrying platform is fixed with the rotation motor of the culture dish carrying platform, the microscope camera carrying platform is positioned under the culture dish carrying platform, and the upper surface of the microscope camera carrying platform is provided with a first microscope camera and a second microscope camera which are distributed oppositely;

the matched gas-liquid flow device is positioned outside the heat insulation sealing cover and comprises a matched gas-liquid flow platform, and a culture solution and paraffin oil heating device, an exhaust gas barrel, a CO (carbon monoxide) are arranged on the matched gas-liquid flow platform₂Gas cylinder, O₂The culture solution and paraffin oil heating device is provided with a paraffin oil barrel, a first culture solution barrel, a second culture solution barrel, a third culture solution barrel and a fourth culture solution barrel; paraffin oil drum with the upper end of paraffin oil application of sample device is connected, No. one culture solution bucket No. two culture solution buckets No. three culture solution buckets with No. four culture solution buckets respectively with a culture solution application of sample pump No. two culture solution application of sample pump No. three culture solution application of sample pump with the upper end of No. four culture solution application of sample pump is connected, CO₂Gas cylinder and said CO₂The air inlet end of the air inlet device is connected with the O₂Gas cylinder and said O₂The air inlet end of the air inlet device is connected, the sterile water barrel is connected with the liquid inlet end of the spraying and humidifying device, the waste gas barrel is connected with the air outlet end of the exhaust device, and the waste liquid barrel is connected with the upper end of the waste discharge device;

a pipeline introducing hole with a sealing effect is formed in the shell of the heat-insulating sealing cover, and the first culture solution barrel, the second culture solution barrel, the third culture solution barrel and the fourth culture solution barrel are respectively connected with the first culture solution sample adding pump, the second culture solution sample adding pump, the third culture solution sample adding pump and the fourth culture solution sample adding pump through culture solution filling pipes connected with each other, paraffin filling pipes connected between the paraffin oil barrel and the paraffin oil sample adding device, and a waste liquid discharge pipe connected between the waste liquid barrel and the waste liquid discharge device, and penetrate into the heat-insulating sealing cover through the pipeline introducing hole so as to ensure the sealing of the pipeline introducing part;

the integrated controller is positioned outside the heat insulation sealing cover and is respectively connected with the CO₂Electrochemical detector of concentration, said CO₂Concentration monitoring light source, humidity monitoring detector, humidity monitoring light source, temperature sensing device, O₂Concentration electrochemical detector, the spray humidifying device, the exhaust device, the dehumidifying device, and the CO₂Air intake device, said O₂Air inlet unit gas heating filters the perfusion device sample adding device fixed station vertical migration motor culture solution sample adding pump, No. two culture solution sample adding pumps, No. three culture solution sample adding pumps, No. four culture solution sample adding pumps, culture solution sample adding device culture dish lighting source, fine cell sample adding device vertical migration motor the waste discharge device vertical migration motor paraffin oil sample adding device vertical migration motor culture dish plummer rotating electrical machines microscope camera microscope stage rotating electrical machines first micro camera, second micro camera with culture solution and paraffin oil heating device electricity are connected to coordinate the linkage work of each above power consumption device and receive the monitoring signal that comes from each detector and micro camera.

Furthermore, the initial position height of culture solution application of sample device application of sample needle is less than the initial position height of sperm cell application of sample device application of sample needle, simultaneously sperm cell application of sample device application of sample needle, waste discharge device inhale the appearance needle with the initial position height of paraffin oil application of sample device application of sample needle is the same.

Furthermore, the middle outer surfaces of the sample adding needle of the culture solution sample adding device and the sample sucking needle of the waste discharge device are provided with sealing rings.

Furthermore, the first microscopic camera and the second microscopic camera respectively adopt two objective lenses with different focal lengths so as to improve the imaging definition and the stereoscopic impression of the microscope as much as possible under the condition of unchanged focusing.

Furthermore, the integrated controller is a control computer or a control unit with a PLC as a core.

Furthermore, the integrated controller is electrically connected with a touch display with a good man-machine interaction interface, so that an operator can set an experiment and display an experiment state conveniently.

A matched culture dish of an automatic IVF culture and time difference analysis device comprises a culture dish body matched with a culture dish bearing table of the automatic IVF culture and time difference analysis device in size, wherein the whole culture dish body is made of transparent materials, and a pair of culture dish clamping columns matched with culture dish clamping grooves of the automatic IVF culture and time difference analysis device are arranged on the outer circumferential side surface of the culture dish body; the upper surface of culture dish body is provided with oil blanket chamber recess, the tank bottom edge of oil blanket chamber recess is provided with several can with automatic IVF cultivate with time difference analytical equipment's sperm cell application of sample device application of sample needle cooperate and have the cultivation cell of spiral flow fluid change function, all is provided with the cultivation cell application of sample hole and cultivation cell row useless hole that are used for forming the spiral fluid flow on every inner wall of cultivateing the cell, the tank bottom middle part of oil blanket chamber recess is provided with can cultivate with automatic IVF with the sealed complex culture dish culture solution application of sample hole of culture solution application of sample device application of time difference analytical equipment, the tank bottom middle part of oil blanket chamber recess is provided with can cultivate with automatic IVF and the sealed complex culture dish waste discharge hole of waste device suction of time difference analytical equipment, the tank bottom below of oil blanket chamber recess is provided with range from top to bottom and each other not communicating culture solution buffer chamber, the upper end of culture solution cushion chamber with culture dish culture solution application of sample hole intercommunication, simultaneously the side of culture solution cushion chamber respectively with every culture cell application of sample hole intercommunication, the upper end of waste liquid cushion chamber with culture dish waste discharge hole intercommunication, the side of waste liquid cushion chamber respectively with every culture cell waste discharge hole intercommunication.

It is further, same in the cultivation cell, cultivate the cell and add the sample hole with it is located respectively to cultivate the cell waste discharge hole cultivate the upper portion and the lower part of cell inner wall, cultivate the opening of cell and add the sample hole with the opening of cultivating the cell and arranging the waste discharge hole all with the inner wall of cultivating the cell is the corner cut, just cultivate the opening of cell and add the sample hole with it is opposite to cultivate the open orientation of cell waste discharge hole to be in when the culture solution trades the spiral flow is formed in the cultivation cell. Cultivate again through automatic IVF and give with time difference analytical equipment's culture solution application of sample device cultivate the malleation of cell application of sample hole and automatic IVF cultivate with time difference analytical equipment's waste discharge device gives cultivate the negative pressure that the cell was arranged waste hole, thereby make each can form the spiral flow in the cell, let every cultivate the culture solution in the cell and all trade the liquid with the form of spiral flow.

We find through experiments that the spiral flow is better in cleaning effect on cumulus granular cells and waste sperm cells compared with laminar flow, the culture solution gradient concentration liquid changing efficiency is also higher, and in addition, the spiral flow can keep fertilized eggs always in the central position of a culture chamber, so that the influence on the fertilized eggs in the division and development process is reduced. Therefore, the innovative spiral flow liquid changing technology can ensure the thorough removal of sperm cells, cumulus granular cells and culture metabolites, and concentration gradient replacement is adopted among different culture solutions, so that the external stimulation to fertilized eggs is reduced to the maximum extent, and the fertilization rate and the blastocyst culture rate are effectively improved.

Furthermore, all the culture cells are uniformly arranged on the outer edge of the bottom of the groove of the oil seal cavity in an annular shape.

Further, the number of the culture chambers is preferably 9, which can meet the requirement of one test of most users

Furthermore, the number of the culture chambers can be increased and the arrangement mode can be changed according to actual needs.

The utility model has the advantages that:

1. the utility model discloses an automatic IVF cultivates and time difference analytical equipment's main part device adopts thermal-insulated sealed design to can effectively maintain its inside CO₂Concentration, O₂Phases of concentration, temperature and humidityThe pair of constant culture dishes are ensured to be in an interference-free culture environment, so that the fully-closed automatic culture of human or animal fertilized eggs and the real-time microscopic observation and evaluation of the culture process can be realized, the attendance of professionals is not needed in the culture period of 4-5 days, the manpower is thoroughly liberated, and the IVF experimental efficiency is greatly improved.

2. The utility model discloses an automatic IVF cultivates and time difference analytical equipment is equipped with real-time temperature, humidity, CO₂Concentration and O₂The concentration monitoring system records the running condition of the equipment and each parameter of the culture environment in real time, and is combined with a touch display with a good human-computer interaction interface, so that the out-of-control and in-time early warning can be realized, and the safety and the reliability of the device are greatly improved.

3. The utility model discloses an automatic IVF cultivates and adopts neotype application of sample and inhale a kind device with time difference analytical equipment, and each application of sample needle height can match supporting culture dish to remove together from top to bottom through a total motor control, wherein an application of sample needle of four kinds of culture solution application of sample devices sharing, thereby save instrument occupation space and cost, and improve cultivation efficiency.

4. The utility model discloses an automatic IVF cultivates and time difference analytical equipment adopts neotype real-time microscopic imaging morphology analytical technique, utilizes two different objective of focus, need not the extra focus of motor regulation, just can promote the definition and the third dimension of microscope formation of image as far as possible under the unchangeable circumstances of focus and satisfy the demand, can high-efficient high-quality shooting real-time cultivation process to supply the IVF doctor to judge the culture quality.

5. The utility model discloses an automatic IVF cultivates and time difference analytical equipment adopts the modularized design, and many sets of main part devices can be connected to one of them suit gas-liquid flow device, and it is convenient to connect, easy operation practices thrift the cost.

6. The utility model discloses a supporting culture dish is according to people's embryonated egg size design, adopt harmless transparent material, every cultivates the cell and is not the not co-altitude and be the cultivation cell application of sample hole of certain opening angle and cultivates the cell and arrange the hole of wasting discharge respectively, malleation and the negative pressure that the waste discharge device gave at the culture solution application of sample device of cooperation subject device, form the spiral flow in each cultivation cell, thereby make each cultivation cell indoor culture solution all trade the liquid with the form of spiral flow, compare the laminar flow, the spiral flow is better to the cleaning performance of cumulus granular cell and abandonment sperm cell, culture solution gradient concentration trades liquid efficiency and is also higher, and the spiral flow can keep the embryonated egg to be in the central point who cultivates the cell always, thereby reduce the influence that the embryonated egg divides the development in-process.

7. The utility model discloses an automatic IVF cultivates and time difference analytical equipment and supporting culture dish adopts the spiral flow of innovation to trade the liquid technique, can guarantee the thorough clearance of sperm cell, cumulus granular cell and culture metabolite, and adopts concentration gradient to change between the different culture solutions, and the maximize reduces the external stimulation to the embryonated egg, has effectively improved fertility rate and blastocyst culture rate.

8. The utility model discloses a cell is cultivateed in preferred 9 holes of supporting culture dish can satisfy the demand of most users once experiments, and operating personnel can be according to different users' demand to select corresponding hole number to test, it is more humanized, and efficiency of software testing is higher.

The above description is only an overview of the technical solution of the present invention, and in order to make the technical means of the present invention clearer and can be implemented according to the content of the description, the following detailed description is made with reference to the preferred embodiments of the present invention and accompanying drawings. The detailed description of the present invention is given by the following examples and the accompanying drawings.

Drawings

The accompanying drawings, which are included to provide a further understanding of the invention and are incorporated in and constitute a part of this application, illustrate embodiment(s) of the invention and together with the description serve to explain the invention without undue limitation to the invention. In the drawings:

FIG. 1 is a perspective view of the main body of the automatic IVF culturing and time difference analyzing apparatus of the present invention after removing a part of the outer shell and the bottom shell;

FIG. 2 is a perspective view of the main body of the automatic IVF culturing and equation of time analyzer shown in FIG. 1 after assembling a matched culture dish;

FIG. 3 is a top view of FIG. 1;

FIG. 4 is a right side view of FIG. 1;

FIG. 5 is a perspective view of the gas-liquid flow device of the automatic IVF culture and time difference analyzer of the present invention;

FIG. 6 is a perspective view of a dish for an automatic IVF culture and time difference analyzer according to the present invention;

FIG. 7 is a vertical sectional perspective view of a dish for an automatic IVF culture and time difference analyzer according to the present invention;

FIG. 8 is a vertical cross-sectional side view of a dish for an automatic IVF culture and time difference analyzer according to the present invention;

FIG. 9 is a simulation diagram of the spiral flow-changing liquid state of the culture chamber of the matched culture dish of the automatic IVF culture and time difference analysis device of the present invention.

Detailed Description

The present invention will be described in detail below with reference to the accompanying drawings in conjunction with embodiments. The description as set forth herein is intended to provide a further understanding of the invention and forms a part of this application and the exemplary embodiments and descriptions thereof are presented for purposes of illustration and description and are not intended to limit the invention in any way.

An automatic IVF culture and time difference analysis device comprises a main body device, a matched gas-liquid flow device and a comprehensive controller.

Referring to fig. 1-4, the main body device includes an openable heat-insulating sealing cover 1, the heat-insulating sealing cover 1 is not fully shown in the figures, a part of the casing and the bottom cover are hidden, and a sample adding device and a culture dish carrying device are arranged in the heat-insulating sealing cover 1; CO is arranged on the inner wall of the heat insulation sealing cover 1₂ Concentration electrochemical detector 2, CO₂Concentration monitoring light source 24, humidity monitoring detector 23, humidity monitoring light source 3, temperature sensing device 20 and O₂Concentration electrochemical detector 8, and said CO₂ Concentration electrochemical detector 2 and said CO₂The concentration monitoring light sources 24 are oppositely arranged, the humidity monitoring detector 23 and the humidity monitoring light sources 3 are oppositely arranged, and the partitionThe housing of the heat seal cover 1 is embedded with a spray humidifier 26, an exhaust device 12, a dehumidifier 13, and CO₂Air intake 29 and O₂An air intake device 30, and the CO₂ Air intake device 29 and said O₂The air inlet end of the air inlet device 30 is connected with the shell of the heat insulation sealing cover 1 after passing through the air heating and filtering perfusion device 27.

The sample adding device is positioned on the upper half part in the heat insulation sealing cover 1, the sample adding device comprises a sample adding device fixing table 7, the sample adding device fixing table 7 is connected with a sample adding device fixing table vertical moving motor 25 arranged on the inner top surface of the heat insulation sealing cover 1 through a sample adding device fixing table support column 35, and the sample adding device fixing table 7 can be vertically movably hung on the upper half part in the heat insulation sealing cover 1 through the matching of the sample adding device fixing table support column 35 and the sample adding device fixing table vertical moving motor 25; the upper surface of the sample adding device fixing table 7 is provided with a first culture solution sample adding pump 18, a second culture solution sample adding pump 19, a third culture solution sample adding pump 21 and a fourth culture solution sample adding pump 22, the lower surface of the sample adding device fixing table 7 is provided with a culture solution sample adding device 62 and a culture dish illumination light source 28, the lower ends of the first culture solution sample adding pump 18, the second culture solution sample adding pump 19, the third culture solution sample adding pump 21 and the fourth culture solution sample adding pump 22 are all connected with a culture solution sample adding needle 17, and the lower end of the culture solution sample adding device 62 is provided with a culture solution sample adding needle 17; and simultaneously, be provided with sperm cell application of sample device 4, the device of wasting discharge 5 and paraffin oil application of sample device 6 that can move perpendicularly through sperm cell application of sample device vertical movement motor 31, the device of wasting discharge vertical movement motor 32 and paraffin oil application of sample device vertical movement motor 33 on the application of sample device fixed station 7 respectively, just sperm cell application of sample device 4 waste discharge device 5 with the lower extreme of paraffin oil application of sample device 6 is provided with sperm cell application of sample device application of sample needle 63, the device of wasting discharge suction appearance needle 64 and paraffin oil application of sample device application of sample needle 16 respectively.

The culture dish bearing device is positioned at the lower half part in the heat insulation sealing cover 1, the culture dish bearing device comprises a round and transparent culture dish bearing table 10, a culture dish bearing table flange 61 is arranged on the circumference of the culture dish bearing table 10, a pair of culture dish clamping grooves 9 for fixing a matched culture dish 36 is arranged on the culture dish bearing table flange 61, the bottom of the culture dish bearing table 10 is connected with a culture dish bearing table rotating motor 15 arranged at the bottom surface in the heat insulation sealing cover 1 through a culture dish bearing table supporting column 60, the culture dish bearing table 10 is horizontally and rotatably arranged at the lower half part in the heat insulation sealing cover 1 through the matching of the culture dish bearing table rotating motor 15 and the culture dish bearing table supporting column 60, a micro camera head supporting column 47 and a micro camera head supporting column 46 are sequentially sleeved on the culture dish bearing table supporting column 60, the upper end of the microscope camera stage support column 47 is connected with the microscope camera stage 46, the lower end of the microscope camera stage support column 47 is connected with the microscope camera stage rotating motor 14, the microscope camera stage rotating motor 14 is fixed with the culture dish bearing table rotating motor 15, the microscope camera stage 46 is located under the culture dish bearing table 10, and the upper surface of the microscope camera stage 46 is provided with a first microscope camera 11 and a second microscope camera 34 which are distributed oppositely.

Referring to fig. 5, the matching gas-liquid flow device is located outside the heat insulation sealing cover 1, the matching gas-liquid flow device comprises a matching gas-liquid flow platform 48, and a culture solution and paraffin oil heating device 49, an exhaust gas barrel 50 and CO are arranged on the matching gas-liquid flow platform 48₂Gas cylinder 54, O₂The culture solution and paraffin oil heating device 49 is provided with a paraffin oil barrel 51, a first culture solution barrel 52, a second culture solution barrel 53, a third culture solution barrel 56 and a fourth culture solution barrel 57; paraffin oil drum 51 with paraffin oil application of sample device 6's upper end is connected, a culture solution bucket 52 No. two culture solution buckets 53 No. three culture solution buckets 56 with No. four culture solution bucket 57 respectively with a culture solution application of sample pump 18 No. two culture solution application of sample pump 19 No. three culture solution application of sample pump 21 with No. four culture solution application of sample pump 22's upper end is connected, CO₂Gas cylinder 54 and the CO₂Air inlet end of air inlet device 29Connection of said O₂Gas cylinder 55 and said O₂The inlet end of air inlet unit 30 is connected, aseptic cask 58 with the inlet end of spraying humidification device 26 is connected, waste gas bucket 50 with exhaust apparatus 12's the end of giving vent to anger is connected, waste liquid bucket 59 with waste discharge apparatus 5's upper end is connected.

Meanwhile, referring to fig. 1-2, a pipeline introducing hole 66 with a sealing effect is formed in the shell of the heat-insulating sealing cover 1, and the first culture solution barrel 52, the second culture solution barrel 53, the third culture solution barrel 56 and the fourth culture solution barrel 57 are respectively connected with the first culture solution sample adding pump 18, the second culture solution sample adding pump 19, the third culture solution sample adding pump 21 and the fourth culture solution sample adding pump 22 through a culture solution filling pipe, a paraffin filling pipe connected between the paraffin oil barrel 51 and the paraffin oil sample adding device 6, and a waste liquid discharging pipe connected between the waste liquid barrel 59 and the waste discharging device 5 are inserted into the heat-insulating sealing cover 1 through the pipeline introducing hole 66 to ensure the sealing of a pipeline introducing part, and the connection of specific pipelines in the figure is not shown.

The integrated controller is positioned outside the heat insulation sealing cover 1, and the integrated controller is respectively connected with the CO₂ Concentration electrochemical detector 2, said CO₂Concentration monitoring light source 24, humidity monitoring detector 23, humidity monitoring light source 3, temperature sensing device 20, O₂Concentration electrochemical detector 8, spray humidifying device 26, exhaust device 12, dehumidifying device 13, and CO₂ Air intake device 29, said O₂ Air inlet unit 30, gas heating filters perfusion device 27, application of sample device fixed station vertical migration motor 25, culture solution application of sample pump 18, No. two culture solution application of sample pumps 19, No. three culture solution application of sample pump 21, No. four culture solution application of sample pump 22, culture solution application of sample device 62, culture dish lighting source 28, sperm cell application of sample device vertical migration motor 31, arrange useless device vertical migration motor 32, paraffin oil application of sample device vertical migration motor 33, culture dish plummer rotating electrical machines 15, micro camera microscope4. The first microscopic camera 11, the second microscopic camera 34 and the culture solution and paraffin oil heating device 49 are electrically connected so as to coordinate the linkage work of the above electric devices and receive monitoring signals from each detector and the microscopic cameras.

Further, the initial position height of the sample adding needle 17 of the culture solution sample adding device is lower than the initial position height of the sample adding needle 63 of the sperm cell sample adding device, and the initial position heights of the sample adding needle 63 of the sperm cell sample adding device, the sample sucking needle 64 of the waste discharging device and the sample adding needle 16 of the paraffin oil sample adding device are the same.

Further, the middle outer surfaces of the sample adding needle 17 of the culture solution sample adding device and the sample sucking needle 64 of the waste discharge device are both provided with sealing rings.

Further, the first microscopic camera 11 and the second microscopic camera 34 respectively adopt two objective lenses with different focal lengths, so that the definition and the stereoscopic impression of the microscope imaging are improved as much as possible under the condition of unchanged focusing.

Referring to fig. 6-8, a culture dish for an automatic IVF culture and time difference analysis device comprises a culture dish body 65 with a size matched with a culture dish bearing platform of the automatic IVF culture and time difference analysis device, wherein the culture dish body 65 is made of a transparent material, and a pair of culture dish clamping columns 41 capable of being matched with culture dish clamping grooves of the automatic IVF culture and time difference analysis device are arranged on the outer circumferential side surface of the culture dish body 65; the upper surface of culture dish body 65 is provided with oil blanket chamber recess 43, the tank bottom edge of oil blanket chamber recess 43 is provided with the several and can cultivates and just has the culture cell 37 of spiral flow fluid changing function with automatic IVF culture and time difference analytical equipment's sperm cell application of sample device application of sample needle cooperatees, all is provided with culture cell application of sample hole 42 and culture cell waste discharge hole 39 that are used for forming the spiral flow on every inner wall of cultivateing cell 37, the tank bottom middle part of oil blanket chamber recess 43 is provided with can cultivate and time difference analytical equipment's culture solution application of sample device application of sample needle seal complex culture dish culture solution application of sample hole 38 with automatic IVF, the tank bottom middle part of oil blanket chamber recess 43 is provided with can cultivate and time difference analytical equipment's waste discharge hole 40 with automatic IVF culture and time difference analytical equipment's waste discharge device suction needle seal complex culture dish, the tank bottom below of oil blanket chamber recess 43 is provided with range from top to bottom and each other culture solution buffer chamber 44 and, the upper end of culture solution buffer chamber 44 with culture dish culture solution application of sample hole 38 intercommunication, simultaneously the side of culture solution buffer chamber 44 respectively with every cultivate cell application of sample hole 42 intercommunication, the upper end of waste liquid buffer chamber 45 with culture dish waste discharge hole 40 intercommunication, the side of waste liquid buffer chamber 45 respectively with every cultivate cell waste discharge hole 39 intercommunication.

Further, referring to FIG. 9, in the same culture chamber 37, the culture chamber sample addition hole 42 and the culture chamber waste discharge hole 39 are respectively located at the upper portion and the lower portion of the inner wall of the culture chamber 37, the opening of the culture chamber sample addition hole 42 and the opening of the culture chamber waste discharge hole 39 are both in a chamfer with respect to the inner wall of the culture chamber 37, and the orientations of the opening of the culture chamber sample addition hole 42 and the opening of the culture chamber waste discharge hole 39 are opposite to each other, so that a spiral flow is formed in the culture chamber 37 when the culture solution is changed. Give through automatic IVF culture again with time difference analytical equipment's culture solution application of sample device cultivate the malleation of cell application of sample hole 42 and automatic IVF culture and time difference analytical equipment's the exhaust device gives cultivate the cell and arrange waste hole 39's negative pressure, thereby make each can form the spiral flow in the cell 37 cultivates, lets every culture solution in the cell 37 all trades the liquid with the form of spiral flow, and the spiral line in figure 9 represents the schematic shape of spiral flow, and the arrow indicates the direction of rotation of spiral flow, and the circular object at center represents oocyte, fertilized egg or morula.

Further, all of the culture cells 37 are uniformly arranged in a ring shape on the outer edge of the bottom of the groove 43 of the oil-sealed chamber.

Further, the number of the culture chambers 37 is preferably 9, which can satisfy the requirement of one test by most users.

Further, the number of the culture chambers 37 can be increased and the arrangement thereof can be changed according to actual needs.

The working principle and the operation method of the utility model are as follows, taking the matched culture dish of the 9-hole culture chamber as an example:

an operator starts the machine, places an empty matched culture dish 36 on the culture dish bearing platform 10, and clamps the matched culture dish clamping column 41 into the matched culture dish clamping groove 9;

placing the test tube filled with the washed sperm cell mixed solution below the sperm cell loading device 4, triggering a sperm cell sample sucking instruction in the integrated controller, and sucking a certain amount of sperm cell mixed solution from the test tube by a loading needle 63 of the sperm cell loading device;

covering the heat insulation sealing cover 1, checking whether the amount of culture solution and gas in each container on the matched gas-liquid flow carrying platform 48 is enough, and whether the containers of waste liquid and waste gas are in an emptying state, and triggering a culture starting instruction in the integrated controller;

the culture solution and paraffin oil heating device 49 starts to work to heat each culture solution barrel and paraffin oil barrel;

the sample adding device fixing table vertically moves the motor 25 to work, the sample adding device fixing table 7 is vertically moved downwards through the sample adding device fixing table supporting column 35, the sample adding device fixing table 7 drives the sperm cell sample adding device 4, the waste discharge device 5, the paraffin oil sample adding device 6, the culture solution sample adding device 62 and the culture dish illuminating light source 28 to move downwards until the sample adding needle 17 of the culture solution sample adding device extends into the culture dish culture solution sample adding hole 38 on the matched culture dish 36 and is sealed;

the first culture solution sample adding pump 18 starts to work, a first culture solution in a first culture solution barrel 52 on the matched gas-liquid flow carrying platform 48 is injected into a culture solution sample adding device 62, then the first culture solution is injected into a culture dish culture solution sample adding hole 38 on a matched culture dish 36 through a sample adding needle 17 of the culture solution sample adding device, then the first culture solution is injected into each culture chamber 37 through a culture solution buffer cavity 44 and a culture chamber sample adding hole 42 in the matched culture dish 36, and after the first culture solution is added to a certain amount, the first culture solution sample adding pump 18 stops working;

the paraffin oil loading device vertically moves the motor 33 to start working, drives the paraffin oil loading device 6 to move downwards for a certain distance, so that the loading needle 16 of the paraffin oil loading device is positioned above the matched culture dish 36, the paraffin oil loading device 6 starts working, paraffin oil in a paraffin oil drum 51 on the matched gas-liquid loading platform 48 is pumped into the paraffin oil loading device 6, and then the paraffin oil is injected into an oil seal cavity groove 43 of the matched culture dish 36 through the loading needle 16 of the paraffin oil loading device;

after the paraffin oil is added to the culture cells which are not submerged in all the culture cells 37, the paraffin oil sample adding device 6 stops working, the paraffin oil sample adding device vertically moves the motor 33 to return, the paraffin oil sample adding device 6 and the paraffin oil sample adding device sample adding needle 16 are driven to return, the sample adding device fixing table vertically moves the motor 25 to return, the sample adding device fixing table 7 is vertically moved upwards through the sample adding device fixing table supporting column 35, and the sample adding device fixing table 7 drives the sperm cell sample adding device 4, the waste discharging device 5, the paraffin oil sample adding device 6, the culture solution sample adding device 62 and the culture dish illumination light source 28 to return upwards;

an operator pauses the instrument, opens the heat insulation sealing cover 1, uses a pipette or an egg peeling needle to respectively add egg cells into each culture chamber (37) in the matched culture dish 36, covers the heat insulation sealing cover 1, and starts the instrument to continue working;

the sperm cell loading device vertically moves the motor 31 to start working, drives the sperm cell loading device 4 to move downwards for a distance, so that the loading needle 63 of the sperm cell loading device extends into the first culture chamber 37 of the matched culture dish 36, the sperm cell loading device 4 starts working, and a certain amount of sperm cell mixed solution is loaded into the first culture chamber 37 through the loading needle 63 of the sperm cell loading device;

the sperm cell sample adding device vertically moves the motor 31 to return to drive the sperm cell sample adding device 4 and the sample adding needle 63 of the sperm cell sample adding device to return, the culture dish bearing table rotating motor 15 starts to work, and the matched culture dish 36 is driven to rotate clockwise by 40 degrees through the culture dish bearing table supporting column 60 and the culture dish bearing table 10;

the sperm cell loading device vertically moves the motor 31 to start working, drives the sperm cell loading device 4 to move downwards for a certain distance, so that the loading needle 63 of the sperm cell loading device extends into the second culture chamber 37 of the matched culture dish 36, the sperm cell loading device 4 starts working, and a certain amount of sperm cell mixed solution is loaded into the second culture chamber 37 through the loading needle 63 of the sperm cell loading device;

the process is circulated until a certain amount of sperm cell mixed solution is added into each of the 9 culture cells 37 of the matched culture dish 36, the sperm cell adding device vertically moves the motor 31 to return to drive the sperm cell adding device 4 and the sperm cell adding needle 63 to return, the rotating motor 15 of the culture dish bearing platform reversely works, and the matched culture dish 36 is driven to rotate anticlockwise for 360 degrees to return through the support column 60 of the culture dish bearing platform and the culture dish bearing platform 10;

after the mixed solution of the ova and the sperm cells is cultured in the first culture solution for a period of time, the sample adding device fixing table vertical moving motor 25 works, the sample adding device fixing table 7 is vertically moved downwards through the sample adding device fixing table supporting column 35, the sample adding device fixing table 7 drives the sperm cell sample adding device 4, the waste discharge device 5, the paraffin oil sample adding device 6, the culture solution sample adding device 62 and the culture dish lighting light source 28 to move downwards until the sample adding needle 17 of the culture solution sample adding device extends into the culture dish culture solution sample adding hole 38 on the matched culture dish 36 and is sealed;

the waste discharge device vertical movement motor 32 starts to work to drive the waste discharge device 5 to move downwards for a certain distance until the sample suction needle 64 of the waste discharge device extends into the culture dish waste discharge hole 40 on the matched culture dish 36 and is sealed;

the second culture solution sample adding pump 19 starts to work, the second culture solution in the second culture solution barrel 53 on the matched gas-liquid flow carrying platform 48 is pumped into the culture solution sample adding device 62, then the second culture solution is injected into each culture cell 37 by the sample adding needle 17 of the culture solution sample adding device through the culture cell culture solution sample adding hole 38, the culture solution buffer cavity 44 and the culture cell sample adding hole 42 on the matched culture cell 36 at a certain flow rate, meanwhile, the waste discharging device 5 starts to work, the waste discharging device sample sucking needle 64 sucks waste liquid of the first culture solution from each culture cell 37 at a certain flow rate through the culture cell waste discharging hole 40, the waste liquid buffer cavity 45 and the culture cell waste discharging hole 39, and a spiral flow is formed in each culture cell 37 under the action of the positive pressure of the culture cell sample adding hole 42 and the negative pressure of the culture cell waste discharging hole 39, so that the spiral flow change of the second culture solution is realized, the sucked first culture solution finally flows into a waste liquid barrel 59 on a matched gas-liquid flow carrier 48 for centralized collection;

until the second culture solution is changed, the waste discharge device vertically moves the motor 32 to return to drive the waste discharge device 5 and the waste discharge device sample suction needle 64 to move upwards; similarly, the sample adding device fixing table vertically moves the motor 25 to return, the sample adding device fixing table 7 vertically moves upwards through the sample adding device fixing table supporting column 35, and the sample adding device fixing table 7 drives the sperm cell sample adding device 4, the waste discharge device 5, the paraffin oil sample adding device 6, the culture solution sample adding device 62 and the culture dish illumination light source 28 to return upwards;

after fertilized eggs are cultured in the second culture solution for a period of time, the sample adding device fixing platform vertically moves the motor 25 to work, the sample adding device fixing platform 7 is vertically moved downwards through the sample adding device fixing platform supporting column 35, the sample adding device fixing platform 7 drives the sperm cell sample adding device 4, the waste discharge device 5, the paraffin oil sample adding device 6, the culture solution sample adding device 62 and the culture dish illumination light source 28 to move downwards until the sample adding needle 17 of the culture solution sample adding device extends into the culture dish culture solution sample adding hole 38 on the matched culture dish 36 and is sealed;

the third culture solution sample adding pump 21 starts to work, the third culture solution in the third culture solution barrel 56 on the matched gas-liquid flow carrying platform 48 is pumped into the culture solution sample adding device 62, then the third culture solution is injected into each culture cell 37 by the sample adding needle 17 of the culture solution sample adding device through the culture cell culture solution sample adding hole 38, the culture solution buffer cavity 44 and the culture cell sample adding hole 42 on the matched culture cell 36 at a certain flow rate, meanwhile, the waste discharging device 5 starts to work, the waste discharging device sample sucking needle 64 sucks waste liquid of the second culture solution from each culture cell 37 at a certain flow rate through the culture cell waste discharging hole 40, the waste liquid buffer cavity 45 and the culture cell waste discharging hole 39, and a spiral flow is formed in each culture cell 37 under the action of the positive pressure of the culture cell sample adding hole 42 and the negative pressure of the culture cell waste discharging hole 39, so that the spiral flow change of the third culture solution is realized, the sucked second culture solution finally flows into a waste liquid barrel 59 on a matched gas-liquid flow carrier 48 for centralized collection;

until the third culture solution is changed, the waste discharge device vertically moves the motor 32 to return to drive the waste discharge device 5 and the waste discharge device sample suction needle 64 to move upwards; similarly, the sample adding device fixing table vertically moves the motor 25 to return, the sample adding device fixing table 7 vertically moves upwards through the sample adding device fixing table supporting column 35, and the sample adding device fixing table 7 drives the sperm cell sample adding device 4, the waste discharge device 5, the paraffin oil sample adding device 6, the culture solution sample adding device 62 and the culture dish illumination light source 28 to return upwards;

after fertilized eggs are cultured in the third culture solution for a period of time, the sample adding device fixing platform vertically moves the motor 25 to work, the sample adding device fixing platform 7 is vertically moved downwards through the sample adding device fixing platform supporting column 35, the sample adding device fixing platform 7 drives the sperm cell sample adding device 4, the waste discharge device 5, the paraffin oil sample adding device 6, the culture solution sample adding device 62 and the culture dish illumination light source 28 to move downwards until the sample adding needle 17 of the culture solution sample adding device extends into the culture dish culture solution sample adding hole 38 on the matched culture dish 36 and is sealed;

the fourth culture solution sample adding pump 22 starts to work, the fourth culture solution in the fourth culture solution barrel 57 on the matched gas-liquid flow carrying platform 48 is pumped into the culture solution sample adding device 62, then the fourth culture solution is injected into each culture cell 37 by the sample adding needle 17 of the culture solution sample adding device through the culture cell culture solution sample adding hole 38, the culture solution buffer cavity 44 and the culture cell sample adding hole 42 on the matched culture cell 36 at a certain flow rate, meanwhile, the waste discharging device 5 starts to work, and the waste discharging device sample sucking needle 64 sucks waste liquid of the third culture solution from each culture cell 37 at a certain flow rate through the culture cell waste discharging hole 40, the waste liquid buffer cavity 45 and the culture cell waste discharging hole 39; at the moment, a spiral flow is formed in each culture small chamber 37 under the action of the positive pressure of a culture small chamber sample adding hole 42 and the negative pressure of a culture small chamber waste discharging hole 39, so that the spiral flow exchange of the No. four culture solution is realized, and the sucked-out No. three culture solution finally flows into a waste liquid barrel 59 on a matched gas-liquid flow carrying platform 48 for centralized collection;

until the fourth culture solution is changed, the waste discharge device vertically moves the motor 32 to return, and drives the waste discharge device 5 and the waste discharge device sample suction needle 64 to move upwards; similarly, the sample adding device fixing table vertically moves the motor 25 to return, the sample adding device fixing table 7 vertically moves upwards through the sample adding device fixing table supporting column 35, and the sample adding device fixing table 7 drives the sperm cell sample adding device 4, the waste discharge device 5, the paraffin oil sample adding device 6, the culture solution sample adding device 62 and the culture dish illumination light source 28 to return upwards;

in the whole culture and liquid change process, the integrated controller respectively controls CO₂ Concentration electrochemical detector 2, CO₂Concentration monitoring light source 24, O₂The concentration electrochemical detector 8, the humidity monitoring detector 23, the humidity monitoring light source 3 and the culture dish illumination light source 28 work continuously to supply CO for the culture environment where the matched culture dish 36 is positioned₂Concentration, O₂Monitoring the concentration, the humidity and the temperature in real time, and providing illumination for a culture environment;

if CO is present₂Concentration electrochemical probe 2 passes across the opposite CO₂The light emitted from the concentration monitoring light source 24 detects CO in the culture environment₂When the concentration exceeds the normal range, the integrated controller controls CO₂Gas inlet means 29 by CO on associated gas-liquid flow stage 48₂The gas cylinder 54 regulates CO of the culture environment₂Concentration until returning to normal range and in CO₂Before entering the heat-insulating sealing cover 1, the CO is filtered by the filter screen of the gas heating and filtering perfusion device 27₂Filtering to prevent large particle impurities or germs from entering the heat insulation seal cover 1;

if O is₂The concentration electrochemical detector 8 detects O in the culture environment₂When the concentration exceeds the normal range, the integrated controller controls O₂Gas inlet means 30 is provided by an O on mating gas-liquid flow stage 48₂ Gas cylinder 55 regulates the O of the culture environment₂Concentration until returning to the normal range and in O₂Before entering the heat-insulating sealing cover 1, the filter net of the gas heating, filtering and filling device 27 will face O₂Filtering to prevent large particle impurities or germs from entering the heat insulation seal cover 1;

if the humidity monitoring detector 23 detects that the humidity in the culture environment exceeds the normal range through the light irradiated by the opposite humidity monitoring light source 3, the integrated controller controls the dehumidifying device 13 or the spray humidifying device 26 to adjust the humidity of the culture environment until the humidity returns to the normal range; the spray humidification device 26 performs spray humidification on the culture environment through a sterile water bucket 58 on the matched gas-liquid flow platform 48;

if the temperature sensing device 20 detects that the temperature in the culture environment exceeds the normal range, the integrated controller controls the gas heating, filtering and filling device 27 to heat CO₂Air intake 29 and CO₂The gas introduced by the gas inlet device 29 is heated, so that the gas injected into the culture environment is heated, and the temperature of the culture environment is adjusted until the temperature returns to the normal range;

in CO₂ Air intake device 29 or O₂The gas inlet device 30 injects gas into the culture environment, and the integrated controller controls the gas exhaust device 12 to exhaust gas to the culture environment in order to maintain the sealed pressure of the culture environment, and the exhaust gas is discharged throughExhaust 12 is drawn into a waste gas bucket 50 on a mating gas-liquid flow stage 48 for centralized collection;

in the whole culture and liquid exchange process, the integrated controller informs and controls the microscope camera stage rotating motor 14, the first microscope camera 11 and the second microscope camera 34 to work continuously, the microscope camera stage rotating motor 14 drives the microscope camera stage 46 to rotate continuously through the microscope camera stage supporting column 47, the microscope camera stage 46 drives the first microscope camera 11 and the second microscope camera 34 to rotate continuously below the matched culture dish 36, each culture chamber 37 of the matched culture dish 36 is shot continuously, and images are transmitted to the integrated controller;

after the culture is finished, an operator pauses the instrument, opens the heat insulation sealing cover 1 and takes out the matched culture dish 36, next step of experimental work is carried out, and the integrated controller displays images on the touch display for the worker to check.

The utility model discloses automatic IVF cultivates and all consumer on the time difference analytical equipment are controlled by comprehensive controller, and comprehensive controller control coordinates all consumer's work according to the experiment procedure of setting for in advance to realize accomplishing the cultivation and the liquid change process of whole human or animal embryonated egg automatically, and maintain supporting culture dish at the CO of relative invariant at whole in-process₂Concentration, O₂Concentration, humidity and temperature.

The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention, and various modifications and changes may be made by those skilled in the art. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. The utility model provides an automatic IVF cultivates and time difference analytical equipment which characterized in that: the device consists of a main body device, a matched gas-liquid flow device and a comprehensive controller;

the main body device comprises an openable heat-insulating sealing cover (1), and a sample adding device is arranged in the heat-insulating sealing cover (1)Placing and carrying a culture dish; CO is arranged on the inner wall of the heat insulation sealing cover (1)₂Concentration electrochemical detector (2), CO₂A concentration monitoring light source (24), a humidity monitoring detector (23), a humidity monitoring light source (3), a temperature sensing device (20) and O₂Concentration electrochemical detector (8), and said CO₂Concentration electrochemical detector (2) and said CO₂Concentration monitoring light source (24) set up in opposite directions, humidity monitoring detector (23) with humidity monitoring light source (3) set up in opposite directions, it is equipped with spraying humidification device (26), exhaust apparatus (12), dehydrating unit (13), CO to inlay on the casing of heat-insulating sealed cowling (1)₂An air intake device (29) and O₂An air intake (30), and the CO₂An air intake (29) and said O₂The air inlet ends of the air inlet devices (30) are connected with the shell of the heat insulation sealing cover (1) through the air heating and filtering filling device (27);

the sample adding device is positioned on the upper half part in the heat insulation sealing cover (1), the sample adding device comprises a sample adding device fixing table (7), the sample adding device fixing table (7) is connected with a sample adding device fixing table vertical moving motor (25) arranged on the inner top surface of the heat insulation sealing cover (1) through a sample adding device fixing table supporting column (35), and the sample adding device fixing table (7) can be vertically movably hung on the upper half part in the heat insulation sealing cover (1) through the matching of the sample adding device fixing table supporting column (35) and the sample adding device fixing table vertical moving motor (25); the upper surface of the sample adding device fixing table (7) is provided with a first culture solution sample adding pump (18), a second culture solution sample adding pump (19), a third culture solution sample adding pump (21) and a fourth culture solution sample adding pump (22), the lower surface of the sample adding device fixing table (7) is provided with a culture solution sample adding device (62) and a culture dish lighting source (28), the lower ends of the first culture solution sample adding pump (18), the second culture solution sample adding pump (19), the third culture solution sample adding pump (21) and the fourth culture solution sample adding pump (22) are all connected with a sample adding needle (17) of the culture solution sample adding device, and the lower end of the culture solution sample adding device (62) is provided with a sample adding needle (17) of the culture solution sample adding device; meanwhile, a sperm cell sample adding device (4), a waste discharging device (5) and a paraffin oil sample adding device (6) which can move vertically are respectively arranged on the sample adding device fixing table (7) through a sperm cell sample adding device vertical moving motor (31), a waste discharging device vertical moving motor (32) and a paraffin oil sample adding device vertical moving motor (33), and the lower ends of the sperm cell sample adding device (4), the waste discharging device (5) and the paraffin oil sample adding device (6) are respectively provided with a sperm cell sample adding device sample adding needle (63), a waste discharging device sample sucking needle (64) and a paraffin oil sample adding device sample adding needle (16);

the culture dish bearing device is positioned at the lower half part in the heat insulation sealing cover (1), the culture dish bearing device comprises a round and transparent culture dish bearing platform (10), a culture dish bearing platform flange (61) is arranged on the circumference of the culture dish bearing platform (10), a pair of culture dish clamping grooves (9) used for fixing a matched culture dish (36) is arranged on the culture dish bearing platform flange (61), the bottom of the culture dish bearing platform (10) is connected with a culture dish bearing platform rotating motor (15) arranged at the bottom surface in the heat insulation sealing cover (1) through a culture dish bearing platform support column (60), the culture dish bearing platform (10) can be horizontally and rotatably arranged at the lower half part in the heat insulation sealing cover (1) through the matching of the culture dish bearing platform rotating motor (15) and the culture dish bearing platform support column (60), a microscope camera stage support column (47) and a microscope camera stage (46) are sequentially sleeved on the culture dish bearing table support column (60), the upper end of the microscope camera stage support column (47) is connected with the microscope camera stage (46), the lower end of the microscope camera stage support column (47) is connected with a microscope camera stage rotating motor (14), the microscope camera stage rotating motor (14) is fixed with the culture dish bearing table rotating motor (15), the microscope camera stage (46) is positioned under the culture dish bearing table (10), and a first microscope camera (11) and a second microscope camera (34) which are distributed oppositely are arranged on the upper surface of the microscope camera stage (46);

the matched gas-liquid flow device is positioned outside the heat insulation sealing cover (1), the matched gas-liquid flow device comprises a matched gas-liquid flow carrying platform (48), and a culture solution and paraffin oil heating device (49), an exhaust gas barrel (50) and CO are arranged on the matched gas-liquid flow carrying platform (48)₂Gas cylinder (54), O₂The culture solution and paraffin oil heating device (49) is provided with a paraffin oil barrel (51), a first culture solution barrel (52), a second culture solution barrel (53), a third culture solution barrel (56) and a fourth culture solution barrel (57); paraffin oil drum (51) with the upper end of paraffin oil application of sample device (6) is connected, culture solution bucket (52) No. two culture solution buckets (53) No. three culture solution buckets (56) and No. four culture solution bucket (57) respectively with a culture solution application of sample pump (18) No. two culture solution application of sample pump (19) No. three culture solution application of sample pump (21) and the upper end of No. four culture solution application of sample pump (22) are connected, CO₂Gas cylinder (54) and the CO₂The air inlet end of the air inlet device (29) is connected, and the O is₂Gas cylinder (55) and the O₂The air inlet end of the air inlet device (30) is connected, the sterile water barrel (58) is connected with the liquid inlet end of the spray humidifying device (26), the waste gas barrel (50) is connected with the air outlet end of the exhaust device (12), and the waste liquid barrel (59) is connected with the upper end of the waste discharge device (5);

a pipeline introducing hole (66) with a sealing effect is formed in the shell of the heat insulation sealing cover (1), the first culture solution barrel (52), the second culture solution barrel (53), the third culture solution barrel (56) and the fourth culture solution barrel (57) are respectively connected with the first culture solution sample adding pump (18), the second culture solution sample adding pump (19), the third culture solution sample adding pump (21) and the fourth culture solution sample adding pump (22) through a culture solution filling pipe, a paraffin filling pipe connected between the paraffin oil barrel (51) and the paraffin oil sample adding device (6) and a waste liquid discharging pipe connected between the waste liquid barrel (59) and the waste discharging device (5) are respectively penetrated into the heat insulation sealing cover (1) through the pipeline introducing hole (66) so as to ensure the sealing of a pipeline introducing part;

the integrated controller is positioned outside the heat insulation sealing cover (1), and the integrated controller is respectively connected with the CO₂Concentration electrochemical detector (2), said CO₂A concentration monitoring light source (24), a humidity monitoring detector (23), a humidity monitoring light source (3), a temperature sensing device (20), O₂A concentration electrochemical detector (8), the spray humidification device (26), the exhaust device (12), the dehumidification device (13), the CO₂An air intake device (29), the O₂An air inlet device (30), a gas heating, filtering and filling device (27), a sample adding device fixing table vertical moving motor (25), a culture solution sample adding pump (18), a culture solution sample adding pump (19), a culture solution sample adding pump (21), a culture solution sample adding pump (22), a culture solution sample adding device (62), a culture dish illuminating light source (28), a sperm cell sample adding device vertical moving motor (31), a waste discharge device vertical moving motor (32), a paraffin oil sample adding device vertical moving motor (33), a culture dish bearing table rotating motor (15), a microscope camera bearing table rotating motor (14), a first microscope camera (11), a second microscope camera (34) and a culture solution and paraffin oil heating device (49) are electrically connected, so as to coordinate the linkage work of the above electric devices and receive monitoring signals from the detectors and the micro camera.

2. The automated IVF culture and moveout analysis apparatus of claim 1, wherein: the initial position height of culture solution application of sample device application of sample needle (17) is less than the initial position height of sperm cell application of sample needle (63), simultaneously sperm cell application of sample needle (63), waste discharge device inhale appearance needle (64) and the initial position height of paraffin oil application of sample device application of sample needle (16) is the same.

3. The automated IVF culture and moveout analysis apparatus of claim 1, wherein: and sealing rings are arranged on the outer surfaces of the middle parts of the sample adding needle (17) of the culture solution sample adding device and the sample sucking needle (64) of the waste discharge device.

4. The automated IVF culture and moveout analysis apparatus of claim 1, wherein: the first microscopic camera (11) and the second microscopic camera (34) respectively adopt two objective lenses with different focal lengths.

5. The automated IVF culture and moveout analysis apparatus of claim 1, wherein: the integrated controller is a control computer or a control unit taking a PLC as a core.

6. The automated IVF culture and moveout analysis apparatus of claim 1, wherein: the integrated controller is also electrically connected with a touch display with a good man-machine interaction interface.

7. A petri dish for use in an automated IVF culture and jet lag analysis device according to any one of claims 1-6, wherein: the culture dish comprises a culture dish body (65) matched with the culture dish bearing table of the automatic IVF culture and time difference analysis device in size, wherein the whole culture dish body (65) is made of transparent materials, and a pair of culture dish clamping columns (41) matched with culture dish clamping grooves of the automatic IVF culture and time difference analysis device are arranged on the outer circumferential side surface of the culture dish body (65); the upper surface of culture dish body (65) is provided with oil blanket chamber recess (43), the tank bottom edge of oil blanket chamber recess (43) is provided with several and can cultivates with automatic IVF and just have spiral flow to trade culture cell (37) of liquid function with the sperm cell application of sample device application of sample needle cooperation of time difference analytical equipment, all be provided with culture cell application of sample hole (42) and culture cell waste discharge hole (39) that are used for forming the spiral flow on the inner wall of every culture cell (37), the tank bottom middle part of oil blanket chamber recess (43) is provided with culture dish culture solution application of sample hole (38) that can cultivate with automatic IVF and seal the sealed complex of culture solution application of sample device application of sample needle with time difference analytical equipment, the tank bottom middle part of oil blanket chamber recess (43) is provided with can cultivate with automatic IVF and seal the sealed complex culture dish waste discharge hole (40) of waste discharge device application of time difference analytical equipment suction sample needle, the tank bottom below of oil blanket chamber recess (43) is provided with culture solution cushion chamber (44) and waste liquid cushion chamber (45) of arranging from top to bottom and each other not communicating, the upper end of culture solution cushion chamber (44) with culture dish culture solution application of sample hole (38) intercommunication, simultaneously the side of culture solution cushion chamber (44) respectively with every culture cell application of sample hole (42) intercommunication, the upper end of waste liquid cushion chamber (45) with culture dish waste discharge hole (40) intercommunication, the side of waste liquid cushion chamber (45) respectively with every culture cell waste discharge hole (39) intercommunication.

8. The Petri dish for automated IVF culture and moveout analysis according to claim 7, wherein: it is same in cultivateing cell (37), cultivate cell application of sample hole (42) with cultivate the cell and arrange useless hole (39) and be located respectively cultivate the upper portion and the lower part of cell (37) inner wall, cultivate the opening of cell application of sample hole (42) with cultivate the opening that the cell arranged useless hole (39) all with the inner wall of cultivateing cell (37) is the corner cut, just cultivate the opening of cell application of sample hole (42) with cultivate the opposite orientation of the opening that the cell arranged useless hole (39) to be in when the culture solution trades form the spiral flow in cultivateing cell (37).

9. The Petri dish for automated IVF culture and moveout analysis according to claim 7, wherein: all the culture cells (37) are uniformly arranged in a ring shape on the outer edge of the bottom of the oil seal cavity groove (43).

10. The Petri dish for automated IVF culture and moveout analysis according to claim 9, wherein: the number of culture chambers (37) is 9.