CN209797991U - In-situ observation cell culture box - Google Patents
In-situ observation cell culture box Download PDFInfo
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- CN209797991U CN209797991U CN201822232248.7U CN201822232248U CN209797991U CN 209797991 U CN209797991 U CN 209797991U CN 201822232248 U CN201822232248 U CN 201822232248U CN 209797991 U CN209797991 U CN 209797991U
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Abstract
The utility model discloses a cell culture case is surveyed to normal position belongs to biological detection technical field. The in-situ observation cell culture box of the utility model comprises a host and a cell culture chamber; the main machine comprises a temperature control device, a gas control device and a liquid control device,Inlet of gas control device and CO2The air inlet is connected, and the outlet is connected with the first air outlet; the inlet of the liquid control device is connected with the water filling port, and the outlet of the liquid control device is connected with the first air outlet; an upper observation window is arranged on an upper cover plate of the cell culture chamber; the bottom of the box body is provided with a lower observation window, and the lower observation window is arranged opposite to the upper observation window; the second air inlet on the box body is communicated with the first air outlet, and the second air outlet is communicated with the first air inlet. The utility model discloses the incubator has less volume, can place on the mesa, when observing under the microscope, need not open the culture room, takes out the cell and goes to observe, only need dispose optical microscope in the below of transparent observation window, can realize the normal position and observe.
Description
Technical Field
The utility model relates to a cell culture case is surveyed to normal position belongs to biological detection technical field.
Background
The cell culture micro-fluidic chip can accurately control cell microenvironment factors such as substance concentration, solution temperature and humidity value, provide a micro and complex structure similar to the living environment of cells in vivo, provide a three-dimensional growth environment to simulate the state of cells in vivo, and investigate cell behaviors such as gene expression, receptor action and other life activities. The cell culture micro-fluidic chip just needs to observe the cell at the cell culture in-process, and current ordinary cell culture case is bulky to when adopting the microscope to observe, need open the culture chamber, take out the cell and go to observe, the operation is complicated, and can interrupt some life activities of cell, be unfavorable for the life activity of continuous observation cell, can cause great influence to the cell even a bit.
SUMMERY OF THE UTILITY MODEL
For solving the technical problem, the utility model provides a cell culture case is surveyd to normal position has less volume, can place on the mesa, and the upper and lower both sides of cell culture room all are equipped with transparent region, and bottom surface transparent region is used for inverting the microscope and observes, and top surface transparent region is used for people's eye to observe, observes time measuring under the microscope moreover, need not like ordinary cell culture case, opens the culture room, takes out the cell to go to observe, only need with whole culture room directly place observe on the microscope objective table can.
The first purpose of the utility model is to provide an in-situ observation cell incubator, which comprises a host and a cell culture chamber;
The main machine comprises a temperature control device, a gas control device, a liquid control device and CO2the water injection device comprises a gas inlet, a first gas outlet and a water injection port; the inlet of the gas control device and the CO2the air inlet is connected with the first air inlet, and the outlet is connected with the first air outlet; the inlet of the liquid control device is connected with the water filling port, and the outlet of the liquid control device is connected with the first air outlet;
The cell culture chamber comprises a box body and an upper cover plate, and a microfluidic chip is placed in the box body; the box body is provided with an even number of liquid pipeline interfaces for liquid transportation during the culture of the microfluidic chip;
An upper observation window is arranged on the upper cover plate; the bottom of the box body is provided with a lower observation window, and the lower observation window is arranged opposite to the upper observation window;
The box body is provided with a second air inlet and a second air outlet, the second air inlet is communicated with the first air outlet, and the second air outlet is communicated with the first air inlet; is used for gas circulation in the whole cell culture device.
Further, the gas control device comprises a carbon dioxide gas loop and a circulating gas loop; the carbon dioxide gas loop comprises a pressure stabilizing valve and an electromagnetic valve which are connected through a gas pipeline, and the pressure stabilizing valve and the CO are connected2the air inlet is connected, and the electromagnetic valve is connected with the second water collecting box; the circulating gas loop comprises a first water collecting box, a condensing module and a first air pump which are sequentially connected through a gas pipeline, and the first water collecting box is connected with a first air inlet; the first air pump is connected with a second air pump. CO 22Gas from CO2the air inlet enters the main machine, and after passing through the pressure stabilizing valve and the electromagnetic valve, the air inlet and the mist coming out of the atomizing box enter the box body through the first air outlet of the main machine. The circulating gas returned from the cell culture chamber enters the host machine through the first air inlet, then enters the second air pump after passing through the first water collecting box, the condensation module and the first air pumpThe two air pumps are connected with the atomization box, and the circulating gas and the mist generated by atomization enter the cell culture chamber through the first air outlet of the main machine.
Furthermore, the gas control device is also provided with CO2a sensor.
Further, the liquid control device comprises an atomization box and a second water collecting box which are connected through a pipeline; the atomization box is connected with a second air pump and is also provided with a water filling port; the second water collecting box is connected with the first air outlet; the second air pump pushes the mist generated by the atomizing box to enter the cell culture chamber from the first air outlet of the host machine through the second water collecting box.
furthermore, a temperature regulation module is also arranged on the cell culture chamber. The temperature regulation module comprises a first heating sheet, a second heating sheet and a heating refrigerator, the heating refrigerator comprises a heating sheet, a refrigerating sheet, a fan and a wind shield, and the wind shield is used for gas convection to enable the wind direction to form laminar flow. The precise control of the temperature is realized through the regulating action of the heating sheet and the heating refrigerator.
Furthermore, the second air inlet is connected with the first air outlet through an air conveying pipeline, and the second air outlet is connected with the first air inlet through an air conveying pipeline.
Furthermore, the upper observation window and the lower observation window are made of transparent materials.
Furthermore, a drain pipe interface is arranged on the cell culture chamber.
Further, said CO2An air filtering membrane is arranged at the air inlet.
further, the host computer on still include the main control board, control temperature control device, gas controlling means and liquid controlling means through the main control board, the main control board can be MCU (singlechip) control. Through the control of the controller, the temperature device of the utility model can set, control and maintain the optimal temperature of cell culture, and can set and control different temperatures according to the types of cultured organisms, and the temperature fluctuation is at plus or minus 1 ℃; the utility model discloses a CO2Can be set, controlled and maintained at randomThe concentration of CO can be set and controlled differently according to the type of organism being cultured2Concentration, wherein the concentration fluctuation is 1 percent plus or minus; the utility model discloses can realize incessant perfusion state in succession, keep the incessant cell culture cavity that updates of culture solution, overcome conventional cell culture case and need regularly change the culture solution to influence the shortcoming of cell normal growth.
Further, the utility model discloses an in situ observation cell culture case can also include high temperature disinfection or ultraviolet sterilization's module for provide cell culture's sterile environment.
furthermore, the cell culture box of the utility model can not only culture animal cells, but also culture tissues, bacteria, viruses and microorganisms, and has broad-spectrum universality.
Further, the cell culture box of the utility model is not only suitable for the micro-fluidic chip, but also suitable for a culture dish, a culture bottle, a porous culture plate and a culture tube.
The utility model has the advantages that:
the in-situ observation cell culture box has smaller volume and good portability, and can be placed on a table top; transparent areas are arranged on the upper side and the lower side of the cell culture chamber, the transparent areas on the bottom surface are used for observation of an inverted microscope, the transparent areas on the top surface are used for observation of human eyes, cell culture growth can be observed in situ and confirmed in real time, positive and negative in-situ observation can be supported, and cells do not need to move in the observation; and the utility model discloses observe time measuring under the microscope, need not like ordinary cell culture case, open the culture room, take out the cell and go to observe, only need dispose optical microscope in the below of transparent observation window, can realize the normal position, incessantly, the growth state of cell is observed and is shot in real time, the operation is more traditional nimble. Furthermore, the utility model discloses can support the cell culture of a plurality of chip regions, and a plurality of cell regions can distinguish each other independently.
Drawings
FIG. 1 is a schematic view of the whole in-situ cell incubator of the present invention;
FIG. 2 is a schematic structural view of the host of the in-situ cell culture box of the present invention;
FIG. 3 is a schematic view of the front structure of the in-situ observation cell incubator body of the present invention;
FIG. 4 is a schematic view of the back structure of the box body of the in-situ observation cell incubator of the present invention;
FIG. 5 is the schematic diagram of the temperature adjustment module of the in-situ observation cell culture box of the present invention.
Detailed Description
The present invention is further described with reference to the following drawings and specific embodiments so that those skilled in the art can better understand the present invention and can implement the present invention, but the embodiments are not to be construed as limiting the present invention.
Example 1:
With reference to the attached drawings, the in-situ observation cell incubator of the utility model comprises a host 1 and a cell culture chamber 2; the main body 1 comprises a temperature control device 3, a gas control device 4 and a liquid control device 5, and CO2The water filling device comprises a gas inlet 6, a first gas inlet 7, a first gas outlet 8 and a water filling port 9; inlet of gas control device 3 and CO2The air inlet 6 is connected with the first air inlet 7, and the outlet is connected with the first air outlet 8; the inlet of the liquid control device 4 is connected with the water filling port 9, and the outlet is connected with the first air outlet 8;
the cell culture chamber 2 comprises a box body 10, an upper cover plate 11 and a drain pipe interface 18, wherein a microfluidic chip is placed in the box body 10; the box body 10 is provided with an even number of liquid pipeline interfaces 12, and one microfluidic chip corresponds to the two liquid pipeline interfaces and is used for liquid conveying during the culture of the microfluidic chip; the upper cover plate 11 is provided with a heating module 17 and an upper observation window 13; the bottom of the box body 10 is provided with a lower observation window 14, the lower observation window 14 is arranged opposite to the upper observation window 13, and the upper observation window and the lower observation window are made of transparent materials; a second air inlet 15 and a second air outlet 16 are arranged on the box body 10, the second air inlet 15 is connected with the first air outlet 8 through an air conveying pipeline, and the second air outlet 16 is connected with the first air inlet 7 through an air conveying pipeline; used for gas circulation in the whole culture device.
In particular toThe gas control device 4 of the present invention comprises a carbon dioxide gas loop and a circulating gas loop; the carbon dioxide gas loop comprises a pressure stabilizing valve 41 and an electromagnetic valve 46 which are connected through a gas pipeline, wherein the pressure stabilizing valve 41 and the CO gas2the air inlet 6 is connected, and the electromagnetic valve 46 is connected with the second water collecting box 52; the circulating gas loop comprises a first water collecting box 42, a condensing module 43 and a first air pump 44 which are connected in sequence through a gas pipeline, and the first water collecting box 42 is connected with the first air inlet 7; the first air pump 44 is connected to a second air pump 53. CO 22gas from CO2The air inlet 6 enters the main machine, passes through the pressure stabilizing valve 41 and the electromagnetic valve 46, and then enters the cell culture chamber together with the mist from the atomizing box 51 through the first air outlet 8 of the main machine. The circulating gas returning from the cell culture chamber enters the main machine through the first air inlet 7, then passes through the first water collecting box 42, the condensation module 43 and the first air pump 44, and is connected to the second air pump 53, the second air pump 53 is connected with the atomization box 51, and the circulating gas and the mist generated by atomization enter the cell culture chamber through the first air outlet 8 of the main machine. CO is also arranged in the gas control device2A sensor 45.
Specifically, the liquid control device 5 of the present invention includes an atomization box 51 and a second water collection box 52 connected by a pipe; the atomization box 51 is connected with a second air pump 53, and the atomization box 51 is also provided with a water filling port 9; the second water collecting box 52 is connected with the first air outlet 8; the second air pump 53 pushes the mist generated by the atomizing box to pass through the second water collecting box and enter the cell culture chamber from the first air outlet 8 of the main machine.
The utility model discloses a still include main control board 19 on the host computer, control temperature control device, gas control device and liquid control device through the main control board, the main control board can be MCU (singlechip) control.
the utility model discloses a be equipped with temperature regulation module 17 on the cell culture case, temperature regulation module 17 includes first heating plate 171, second heating plate 172 and heating refrigerator 173, and the heating refrigerator includes heating/cold piece 1731, fan 1732 and deep bead 1733, and the deep bead is used for the gas convection, lets the wind direction form the laminar flow. The precise control of the temperature is realized through the regulating action of the heating sheet and the heating refrigerator.
The utility model discloses a cell culture case is surveyed to normal position can also include high temperature disinfection or ultraviolet sterilization's module for provide cell culture's sterile environment.
In the working process of the cell culture box, the microfluidic chip is arranged in the cell culture chamber, and culture solution is conveyed to the microfluidic chip through the liquid pipeline interface; then CO is introduced2Introducing a gas control device, adding water into a liquid control device, atomizing into mist, and introducing CO2Introducing into the cell culture chamber together with the mist, and controlling the temperature of the cell culture chamber to provide stable temperature, humidity and CO for the cell culture chamber2A concentration parameter. When observing, the transparent region of the observation window under the bottom surface is used for observing by an inverted microscope, the transparent region of the observation window on the top surface is used for observing by human eyes, and when observing under the microscope, the culture chamber does not need to be opened like a common cell culture box, cells are taken out to be observed, and only the whole culture chamber needs to be directly placed on a microscope objective table for observation.
The cell culture box of the utility model can not only culture animal cells, but also culture tissues, bacteria, viruses and microorganisms, and has broad-spectrum universality. Furthermore, the cell culture box of the utility model is not only suitable for the micro-fluidic chip, but also suitable for the culture dish, the culture bottle, the porous culture plate and the culture tube.
The above-mentioned embodiments are merely preferred embodiments for fully illustrating the present invention, and the scope of the present invention is not limited thereto. Equivalent substitutes or changes made by the technical personnel in the technical field on the basis of the utility model are all within the protection scope of the utility model. The protection scope of the present invention is subject to the claims.
Claims (10)
1. An in-situ observation cell culture box is characterized by comprising a host and a cell culture chamber;
the main machine comprises a temperature control device, a gas control device, a liquid control device and CO2The water injection device comprises a gas inlet, a first gas outlet and a water injection port; said gasInlet of body control device and CO2The air inlet is connected with the first air inlet, and the outlet is connected with the first air outlet; the inlet of the liquid control device is connected with the water filling port, and the outlet of the liquid control device is connected with the first air outlet;
The cell culture chamber comprises a box body and an upper cover plate, and a microfluidic chip is placed in the box body; the box body is provided with an even number of liquid pipeline interfaces;
An upper observation window is arranged on the upper cover plate; the bottom of the box body is provided with a lower observation window, and the lower observation window is arranged opposite to the upper observation window;
The box body is provided with a second air inlet and a second air outlet, the second air inlet is communicated with the first air outlet, and the second air outlet is communicated with the first air inlet.
2. The in situ cell culture incubator of claim 1, wherein the gas control device comprises a carbon dioxide gas loop and a recycle gas loop; the carbon dioxide gas loop comprises a pressure stabilizing valve and an electromagnetic valve which are connected through a gas pipeline, and the pressure stabilizing valve and the CO are connected2The air inlet is connected, and the electromagnetic valve is connected with the second water collecting box; the circulating gas loop comprises a first water collecting box, a condensing module and a first air pump which are sequentially connected through a gas pipeline, and the first water collecting box is connected with a first air inlet; the first air pump is connected with a second air pump.
3. The in situ cell culture incubator as recited in claim 2, wherein the gas control device further comprises CO2A sensor.
4. The in situ observation cell culture chamber of claim 1, wherein the liquid control device comprises an atomization box and a second water collection box which are connected through a pipeline; the atomization box is connected with a second air pump and is also provided with a water filling port; the second water collecting box is connected with the first air outlet.
5. The in situ cell culture incubator according to claim 1, wherein the cell culture chamber is further provided with a temperature regulation module.
6. The in situ observation cell culture chamber of claim 1, wherein the second gas inlet is connected to the first gas outlet, and the second gas outlet is connected to the first gas outlet via a gas transmission pipeline.
7. the in situ cell culture box according to claim 1, wherein the upper and lower windows are made of transparent material.
8. The in situ cell culture incubator according to claim 1, wherein the cell culture chamber is provided with a drain port.
9. The in situ cell culture chamber of claim 1, wherein the CO is present in a concentration sufficient to prevent the growth of the cells2An air filtering membrane is arranged at the air inlet.
10. The in-situ observation cell culture box of claim 1, wherein the main frame further comprises a main control board, and the temperature control device, the gas control device and the liquid control device are controlled by the main control board.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201822232248.7U CN209797991U (en) | 2018-12-28 | 2018-12-28 | In-situ observation cell culture box |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201822232248.7U CN209797991U (en) | 2018-12-28 | 2018-12-28 | In-situ observation cell culture box |
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| CN209797991U true CN209797991U (en) | 2019-12-17 |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111004721A (en) * | 2019-12-24 | 2020-04-14 | 南通大学 | Ultrathin visual temperature control device suitable for microscope online in-situ observation |
| CN111205980A (en) * | 2020-01-20 | 2020-05-29 | 河海大学常州校区 | Cell culture device for single cell in-situ real-time microscopic observation and operation method thereof |
| CN111849767A (en) * | 2020-06-24 | 2020-10-30 | 东南大学 | Three-dimensional micro-fluidic chip level cell culture case |
| CN111876325A (en) * | 2020-07-16 | 2020-11-03 | 北京中聚高科科技有限公司 | Photoelectric comprehensive test platform capable of providing culture environment for microorganisms |
| CN113512496A (en) * | 2021-07-09 | 2021-10-19 | 中国科学院过程工程研究所 | Device for in-situ culture, staining and observation of cells for inverted microscope and use method and application thereof |
| CN114149920A (en) * | 2021-12-08 | 2022-03-08 | 上海睿钰生物科技有限公司 | Perfusion culture device |
| CN115558587A (en) * | 2022-10-20 | 2023-01-03 | 中国科学院苏州生物医学工程技术研究所 | In-situ culture device for microorganism observation and control method |
-
2018
- 2018-12-28 CN CN201822232248.7U patent/CN209797991U/en active Active
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111004721A (en) * | 2019-12-24 | 2020-04-14 | 南通大学 | Ultrathin visual temperature control device suitable for microscope online in-situ observation |
| CN111205980A (en) * | 2020-01-20 | 2020-05-29 | 河海大学常州校区 | Cell culture device for single cell in-situ real-time microscopic observation and operation method thereof |
| CN111205980B (en) * | 2020-01-20 | 2024-01-26 | 河海大学常州校区 | Cell culture device for single cell in-situ real-time microscopic observation and operation method thereof |
| CN111849767A (en) * | 2020-06-24 | 2020-10-30 | 东南大学 | Three-dimensional micro-fluidic chip level cell culture case |
| CN111876325A (en) * | 2020-07-16 | 2020-11-03 | 北京中聚高科科技有限公司 | Photoelectric comprehensive test platform capable of providing culture environment for microorganisms |
| CN113512496A (en) * | 2021-07-09 | 2021-10-19 | 中国科学院过程工程研究所 | Device for in-situ culture, staining and observation of cells for inverted microscope and use method and application thereof |
| CN114149920A (en) * | 2021-12-08 | 2022-03-08 | 上海睿钰生物科技有限公司 | Perfusion culture device |
| CN115558587A (en) * | 2022-10-20 | 2023-01-03 | 中国科学院苏州生物医学工程技术研究所 | In-situ culture device for microorganism observation and control method |
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