CN104893974B - Can online dynamic realtime monitoring portable expansion of stem cells culture device - Google Patents
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
- C12M41/34—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of gas
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- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
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Abstract
The cell microgravity effect produced the present invention relates to a kind of revolution of utilization cell sample suppress stem cell differentiation stem cell cultivate amplification can dynamic realtime monitoring online portable expansion of stem cells culture device, including the elongated cylindricals revolution cell culture containers of 4 independent rotations, detection sensor array, detection container, nutrient solution composition regulating system, culture loop, parts motion and support structure, base composition；The cell microgravity effect produced is turned round using cell sample and suppresses stem cell differentiation in amplification procedure is cultivated, also it is integrated with the autonomous investigation system of cell culture environment and on-line parameter monitoring system, realize independent development stem cell culture, and in expansion of stem cells cultivating process, can dynamic realtime monitoring relevant parameter, tracking stem cell cultivation amplification procedure；The culture circuit element that all and cell culture fluid including cell culture container is directly contacted uses first use, the convenience that the lifting present invention is operated.
Description
Technical field
It is particularly a kind of to make cell generation simulation micro- heavy based on sample revolution the present invention relates to a kind of expansion of stem cells equipment
Stress effect and ensure that stem cell is suppressed in amplification procedure differentiation, and cell amplification cultivation environment is automatically controlled, key index
The portable expansion of stem cells equipment of the real-time ground-wire monitoring of energy.
Background technology
In the research fields such as Present clinical medical science, life science, cell biology, stem cell largely expands cultivation right and wrong
Often important work.But it can break up in expansion of stem cells incubation, cause expansion of stem cells culture to be lost so as to lose " dryness "
Lose.It is main at present by the way of addition medicine in order to ensure undifferentiated in stem cell incubation, but the medicine of addition is dry
Cell clinical practice brings great risk, makes to break up the stem cell cultivated in terms of clinical practice by very big using Drug inhibition
Constraint.
Existing stem cell culture device does not have the autonomous ability of regulation and control of cell culture environment on the market, and equipment normal work is also
The auxiliary equipments such as dependent cells incubator, this makes equipment in operability with being affected in terms of application field.
Perfect on-line parameter monitoring system is configured without due to having stem cell culture device at present, this makes stem cell
It can not be monitored in real time in amplification cultivating process.
Existing stem cell cultivates stem cell culturing room in equipment and is not combined formula design, completes a cell culture
Afterwards, it is desirable to which whole cell culture chamber is cleaned.
In view of above-mentioned defect, the design people is actively subject to research and innovation, can dynamic realtime online to found one kind
The portable expansion of stem cells culture device of monitoring, makes it with more the value in industry.
The content of the invention
In order to solve the above technical problems, it is an object of the invention to provide the cell that a kind of revolution of utilization cell sample is produced is micro-
The stem cell that gravitational effect suppresses stem cell differentiation cultivates augmentation apparatus；Also be integrated with the autonomous investigation system of cell culture environment with
On-line parameter monitoring system, realizes independent development stem cell culture, is without relying on other periphery auxiliary stand equipment
Stem cell training can be carried out, and in expansion of stem cells cultivating process, can dynamic realtime monitoring relevant parameter, tracking is dry
Cell culture amplification procedure；Meanwhile, what the present invention included including cell culture container all directly contacts with cell culture fluid
Part use first use equipment, lifted this operation convenience can online dynamic realtime monitor portable stem cell
Amplification cultivation equipment.
The present invention can dynamic realtime monitoring online portable expansion of stem cells culture device, including 4 independent rotate
Elongated cylindrical revolution cell culture container, detection sensor array, detection container, nutrient solution composition regulating system, cultivate back
Road, control display system, part motion and support structure and base；The detection sensor array is installed on detection container
On, the culture loop, detection container and nutrient solution composition regulating system are connected into nutrient solution flow circuits by pipeline；It is described
Part motion is made up of with support structure timing belt, synchronous pulley, motor and X-type support, and X-type support is installed on base both sides；4
The elongated cylindrical revolution cell culture container of individual independent rotation is installed on four end points of X-type support by bearing, is driven by motor
Elongated cylindrical revolution cell culture container is rotated；Display system is controlled to include control circuit board, switch and touch-screen, control circuit
Plate is installed on base rear portion；Touch-screen is installed on base front window；Motor is installed on base top.
Further, elongated cylindrical revolution cell culture container includes inner cylinder, outer barrel and the closure at two ends, inner cylinder appearance
Face coats space between thermal control coating, inner/outer tube and vacuumizes to form vacuum heat-insulating layer.
Further, the detection sensor display includes temperature sensor, CO2Concentration sensor, O2Concentration sensor,
Urea concentration sensor, lactic acid concn sensor, kind of sensor can increase and decrease with quantity according to requirement of experiment；Detection sensor is old
Row include all the sensors and are mounted in detection container.
Further, described nutrient solution composition regulating system is by adjuster, filter, CO2Gas cylinder, O2Gas cylinder, N2Gas
Bottle, pressure-reducing valve and appendix composition (species and quantity of gas can increase and decrease according to requirement of experiment)；CO2Gas cylinder, O2Gas cylinder, N2Gas
Bottle is connected to after filter by corresponding pressure-reducing valve by appendix, then is connected to the corresponding air inlet of adjuster parallel；By adjusting
Save device exhaust outlet discharge excessive gas.
Further, culture loop is made up of nutrient solution reservoir, peristaltic pump, cell culture bags and pipeline.
Further, cell culture bags are by bag, nutrient solution anemostat, nutrient solution collecting pipe, coupling device and note liquid taking port
Composition；Nutrient solution anemostat with and nutrient solution collecting pipe be connected respectively by two of coupling device and cell culture bags；Cell is trained
Support bag disposable.
Further, all culture circuit assemblies, detection container and adjuster are connected into nutrient solution by pipeline and flowed back
Road.Pipeline presses nutrient solution reservoir, peristaltic pump, nutrient solution anemostat, nutrient solution collecting pipe, detection container, adjuster, nutrient solution
Reservoir is linked in sequence, and cell culture fluid is carried out in cell culture bags by peristaltic pump drives edge pipeline by nutrient solution anemostat,
After nutrient solution is flowed out by nutrient solution collecting pipe in cell culture bags, into detection container, the sensor requirements of detection container are examined
Each parameter in nutrient solution is surveyed, during the nutrient solution flowed out from detection container directly carries out adjuster, is adjusted as desired and is trained
Part, last nutrient solution is flowed back in reservoir.
By such scheme, the present invention at least has advantages below：
1st, cell culture loop is constituted comprising disposable cell bags, equipment continuous several times carry out stem cell incubation
In, without cleaning, directly change cell culture circuit assemblies；
2nd, can by nutrient solution composition regulating system regulation culture liquid composition, thermal control coating regulating cell culture tube temperature,
So as to realize independent regulation cell culture environment parameter, make it possible the independent work of equipment；
3rd, the present invention turns round microgravity effect using the simulation of cell and suppresses to break up during cell culture come dry, stem cell
Any medicine need not be added in cultivating process, it is ensured that the stem cell cultivated is avoided without clinical practice.
Described above is only the general introduction of technical solution of the present invention, in order to better understand the technological means of the present invention,
And can be practiced according to the content of specification, below with presently preferred embodiments of the present invention and coordinate accompanying drawing describe in detail as after.
Brief description of the drawings
Fig. 1 be the present invention can online dynamic realtime monitor portable expansion of stem cells culture device structural representation；
Fig. 2 is cell culture container and cell culture bags structural representation in the present invention.
Embodiment
With reference to the accompanying drawings and examples, the embodiment to the present invention is described in further detail.Implement below
Example is used to illustrate the present invention, but is not limited to the scope of the present invention.
Referring to Fig. 1 and Fig. 2, described in a preferred embodiment of the present invention it is a kind of can the portable of dynamic realtime monitoring online do
Cell amplification cultivation equipment, including the elongated cylindricals revolution cell culture containers 1 of 4 independent rotations, detection sensor array 2,
Detection container 3, nutrient solution composition regulating system 8, culture loop, control display system, part motion and support structure and bottom
Seat 6；Detection sensor array is installed in detection container, and culture loop, detection container and nutrient solution composition regulating system pass through
Pipeline is connected into nutrient solution flow circuits；Part is moved with support structure by timing belt 41, synchronous pulley 42, motor 43 and X-type
Support 44 is constituted, and X-type support is installed on base both sides；The elongated cylindrical revolution cell culture container of 4 independent rotations passes through axle
Hold and be installed on four end points of X-type support, drive elongated cylindrical revolution cell culture container to rotate by motor；Control display system
Including control circuit board, switch 51 and touch-screen 52, control circuit board is installed on base rear portion；It is anterior that touch-screen is installed on base
Window；Motor is installed on base top.
Elongated cylindrical revolution cell culture container includes the closure 13 of inner cylinder 11, outer barrel 12 and two ends.Interior tube outer surface
Space between thermal control coating 14, inner/outer tube is coated to vacuumize to form vacuum heat-insulating layer.
Detection sensor display includes temperature sensor, CO2Concentration sensor, O2Concentration sensor, urea concentration sensing
Device, lactic acid concn sensor, can increase and decrease coherent detection sensor according to requirement of experiment.Detection sensor display includes all sensings
Device is mounted in detection container.
Nutrient solution composition regulating system is by adjuster, filter, CO2Gas cylinder, O2Gas cylinder, N2Gas cylinder, pressure-reducing valve and appendix
Composition.CO2Gas cylinder, O2Gas cylinder, N2Gas cylinder is connected to after filter by corresponding pressure-reducing valve by appendix, then is connected to parallel
The corresponding air inlet of adjuster；Excessive gas is discharged by adjuster exhaust outlet.
Culture loop is made up of nutrient solution reservoir 71, peristaltic pump 72, cell culture bags and pipeline 73.
Cell culture bags are by bag 81, nutrient solution anemostat 82, nutrient solution collecting pipe 83, coupling device 84 and note liquid taking port 85
Composition.Nutrient solution anemostat with and nutrient solution collecting pipe be connected respectively by two of coupling device and cell culture bags.Cell is trained
Support bag disposable.
Pipeline 73 presses nutrient solution reservoir, peristaltic pump, nutrient solution anemostat, nutrient solution collecting pipe, detection container, regulation
Device, nutrient solution reservoir are linked in sequence.
The operation principle of the present invention is as follows：
Turn on the power switch, equipment display parameters inputting interface on the touchscreen, can be according to requirement of experiment input experiment ginseng
Number；After the start key of inputting interface, equipment is operated pattern automatically：Elongated cylindrical is turned round cell culture container and passed synchronous
Under dynamic system drives, start to rotate, the thermal control coating on interior tube outer surface surface is begun to warm up, cell culture container is maintained 37
DEG C, cell culture fluid is carried out in cell culture bags by peristaltic pump drives edge pipeline by nutrient solution anemostat, is trained in cell culture bags
After nutrient solution is flowed out by nutrient solution collecting pipe, into detection container, respectively join in the sensor requirements detection nutrient solution of detection container
Number, during the nutrient solution flowed out from detection container directly carries out adjuster, is adjusted as desired culture composition, last nutrient solution stream
Return in reservoir.
The present invention is turned round the cell microgravity effect produced using cell sample and suppresses stem cell in amplification procedure is cultivated
Differentiation, is also integrated with the autonomous investigation system of cell culture environment and on-line parameter monitoring system, realizes that independent carry out does
Cell culture, stem cell training can be carried out without relying on other periphery auxiliary stand equipment, and in expansion of stem cells
, can dynamic realtime monitoring relevant parameter, tracking stem cell cultivation amplification procedure in cultivating process；Including cell culture container
All first use, the convenience that the lifting present invention is operated are used with cell culture fluid is directly contacted culture circuit element.
Described above is only the preferred embodiment of the present invention, is not intended to limit the invention, it is noted that for this skill
For the those of ordinary skill in art field, without departing from the technical principles of the invention, can also make it is some improvement and
Modification, these improvement and modification also should be regarded as protection scope of the present invention.
Claims (2)
1. it is a kind of can online dynamic realtime monitoring portable expansion of stem cells culture device, it is characterised in that：Including 4 independences
The elongated cylindrical revolution cell culture container of rotation, detection sensor array, detection container, nutrient solution composition regulating system, training
Support loop, control display system, part motion and support structure and base；The detection sensor array, which is installed on detection, to be held
On device, the culture loop, detection container and nutrient solution composition regulating system are connected into nutrient solution flow circuits by pipeline；Institute
State part motion to be made up of timing belt, synchronous pulley, motor and X-type support with support structure, X-type support is installed on base two
Side；The elongated cylindrical revolution cell culture container of 4 independent rotations is installed on four end points of X-type support by bearing, by motor
Elongated cylindrical revolution cell culture container is driven to rotate；Display system is controlled to include control circuit board, switch and touch-screen, control
Wiring board is installed on base rear portion；Touch-screen is installed on base front window；Motor is installed on base top；The culture loop
It is made up of nutrient solution reservoir, peristaltic pump, cell culture bags and pipeline；The cell culture bags by bag, nutrient solution anemostat,
Nutrient solution collecting pipe, coupling device and note liquid taking port composition；Nutrient solution anemostat with and nutrient solution collecting pipe pass through coupling device respectively
It is connected with two of cell culture bags；Cell culture bags are disposable；Pipeline presses nutrient solution reservoir, peristaltic pump, nutrient solution
Anemostat, nutrient solution collecting pipe, detection container, adjuster, nutrient solution reservoir are linked in sequence, and cell culture fluid is by peristaltic pump
Drives edge pipeline is carried out in cell culture bags by nutrient solution anemostat, and nutrient solution passes through nutrient solution collecting pipe stream in cell culture bags
After going out, into detection container, the sensor requirements of detection container detect each parameter in nutrient solution, the culture flowed out from detection container
Liquid is directly carried out in adjuster, and culture composition is adjusted as desired, and last nutrient solution is flowed back in reservoir；Described nutrient solution
Composition regulating system is by adjuster, filter, CO2Gas cylinder, O2Gas cylinder, N2Gas cylinder, pressure-reducing valve and appendix composition；CO2Gas cylinder, O2
Gas cylinder, N2Gas cylinder is connected to after filter by corresponding pressure-reducing valve by appendix, then is connected to adjuster parallel and is entered accordingly
Gas port；Excessive gas is discharged by adjuster exhaust outlet；The elongated cylindrical revolution cell culture container includes inner cylinder, outer barrel and two
The closure at end；Interior tube outer surface coats space between thermal control coating, inner/outer tube and vacuumizes to form vacuum heat-insulating layer.
2. it is according to claim 1 can online dynamic realtime monitoring portable expansion of stem cells culture device, its feature
It is：The detection sensor display includes temperature sensor, CO2Concentration sensor, O2Concentration sensor, urea concentration sensing
Device, lactic acid concn sensor, kind of sensor can increase and decrease with quantity according to requirement of experiment.
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Families Citing this family (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE102015119756B3 (en) * | 2015-11-16 | 2016-11-17 | Sartorius Stedim Biotech Gmbh | Mixing device for mixing a bioreactor comprising a synchronization device |
| CN106222084B (en) * | 2016-08-04 | 2018-08-21 | 天信和(苏州)生物科技有限公司 | Real-time online process data applied to cell culture preserves system |
| CN108504568B (en) * | 2017-04-14 | 2020-07-17 | 深圳市太空科技南方研究院 | Stem cell automatic amplification culture equipment based on modularization |
| CN106929415B (en) * | 2017-04-14 | 2020-07-21 | 深圳市太空科技南方研究院 | Manual stem cell amplification culture equipment based on modularization |
| CN109306324B (en) * | 2017-07-26 | 2023-07-04 | 广州中国科学院先进技术研究所 | Cell culture and detection device |
| CN107904167B (en) * | 2017-11-15 | 2023-06-06 | 中国航天员科研训练中心 | Automatic cell expansion culture equipment |
| CN110129198A (en) * | 2019-05-15 | 2019-08-16 | 林伟阳 | A kind of stem cell neural network based culture simulated environment data monitoring and prediction embedded subsystem |
| CN111088157A (en) * | 2020-01-09 | 2020-05-01 | 中国人民解放军63919部队 | Low gravity environment simulation system |
| CN113930381A (en) * | 2021-10-30 | 2022-01-14 | 王翠玲 | Stem cell culture method |
| CN114181828B (en) * | 2021-12-03 | 2022-10-28 | 深圳市新一仑生物科技有限公司 | Stem cell automatic amplification culture equipment based on modularization |
| CN114107052B (en) * | 2021-12-03 | 2022-05-20 | 深圳市新一仑生物科技有限公司 | Portable stem cell amplification culture equipment capable of realizing online dynamic real-time monitoring |
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|---|---|---|---|---|
| US5139953A (en) * | 1989-08-29 | 1992-08-18 | Snow Brand Milk Products Co., Ltd. | Rotary column reactor |
| CN101560469A (en) * | 2008-04-14 | 2009-10-21 | 上海交通大学医学院附属上海儿童医学中心 | Rotary dual-chamber stem cell synchronous augmentation and division inoculator |
| CN102174395A (en) * | 2011-01-30 | 2011-09-07 | 中国科学院广州生物医药与健康研究院 | Automation augmentation and culture system of induced pluripotent stem cells |
| CN102181364A (en) * | 2011-03-11 | 2011-09-14 | 北京航空航天大学 | Device for culturing in vitro cells through single axis rotating and online shearing |
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