CN204311067U - A kind of organizational project stem cell inducing cardiomyocytes is cultivated and is measured bio-reactor - Google Patents
A kind of organizational project stem cell inducing cardiomyocytes is cultivated and is measured bio-reactor Download PDFInfo
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- CN204311067U CN204311067U CN201420700163.6U CN201420700163U CN204311067U CN 204311067 U CN204311067 U CN 204311067U CN 201420700163 U CN201420700163 U CN 201420700163U CN 204311067 U CN204311067 U CN 204311067U
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- culturing room
- nutrient solution
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Abstract
The utility model relates to a kind of organizational project stem cell inducing cardiomyocytes and cultivates and measure bio-reactor.The utility model comprises cultivates unit, control unit and measuring unit.Described cultivation unit comprises multiple container for storing liquid and a culturing room, and each container for storing liquid connects corresponding drain pipe by respective jointing, and nutrient solution is inputed to control unit; Control unit controls the nutrient solution entering culturing room; Waste liquid tank is provided with, culturing room and waste liquid tank UNICOM below described culturing room.The utility model can according to regulating the uninterrupted of input nutrient solution, cultured myocardial in culturing room; Can measure in real time beat intensity and the frequency of myocardial cell simultaneously, the final synchronism realizing cultivating and measure, to guarantee to turn out the myocardial cell with excellent mechanical performances, because device have employed high-precision metrical instrument, be provided with minute-pressure setting device, the data recorded are accurately reliably.
Description
Technical field
The utility model belongs to biomedical engineering field, relates to a kind of organizational project stem cell inducing cardiomyocytes and cultivates and measure bio-reactor.
Background technology
Cell therapy to heart stalk and whole latter stage cardiac disorder treatment there are bright prospects, but current effect is clinically not bery desirable, its one of the main reasons is exactly that the mechanical property of the myocardial cell of vitro culture is not mated with host myocardial cells, therefore research and development can be carried out measuring in real time and the bio-reactor regulated to myocardial cells culture process, significant to the application of cell therapy on clinical medicine.
The most important function of myocardial cell is exactly pulsation, i.e. regular contractile motion, therefore measuring the mechanical characteristic of myocardial cell most important is exactly measure its beat intensity and Beating Rate, still can measure the measuring apparatus of its mechanical characteristic at present in culturing process in real time.
Summary of the invention
The utility model, for the deficiencies in the prior art, provides a kind of organizational project stem cell inducing cardiomyocytes and cultivates and measure bio-reactor.
The technical scheme that the utility model technical solution problem is taked is:
The utility model comprises cultivates unit, control unit and measuring unit.
Described cultivation unit comprises multiple container for storing liquid and a culturing room, and each container for storing liquid connects corresponding drain pipe by respective jointing, and nutrient solution is inputed to control unit; Control unit controls the nutrient solution entering culturing room; Waste liquid tank is provided with, culturing room and waste liquid tank UNICOM below described culturing room.
Described control unit comprises Flow-rate adjustment cam and drives the steering wheel of Flow-rate adjustment cam; The flow inputing to each road nutrient solution of control unit is controlled by Flow-rate adjustment cam, and steering wheel is arranged on the top of Flow-rate adjustment cam, and steering wheel is controlled by surface-mounted integrated circuit, and surface-mounted integrated circuit is circumscribed with touch-screen.
Described measuring unit comprises culturing room's sensor and fixes barrier film, sensor fixation plate and piezoelectric transducer; Piezoelectric transducer is fixed barrier film by sensor and is fixed on sensor fixation plate, and sensor fixation plate is between culturing room and waste liquid tank, and piezoelectric transducer is used for beating of perception myocardial cell.
The utility model can according to regulating the uninterrupted of input nutrient solution, cultured myocardial in culturing room; Can measure in real time beat intensity and the frequency of myocardial cell simultaneously, the final synchronism realizing cultivating and measure, to guarantee to turn out the myocardial cell with excellent mechanical performances, because device have employed high-precision metrical instrument, be provided with minute-pressure setting device, the data recorded are accurately reliably.Be synchro measure to stem cell inducing culture myocardial cell and myocardial cell's pulsating force and frequency parameter simultaneously, the intensity of beating of observing culturing process cardiac myocyte inducing culture can be shown intuitively, and can Beating Rate be obtained as calculated.
Accompanying drawing explanation
Fig. 1 is that organizational project stem cell inducing cardiomyocytes is cultivated and measures bioreactor construction figure;
Fig. 2 is that organizational project stem cell inducing cardiomyocytes is cultivated and measures bio-reactor sectional view;
Fig. 3 is that organizational project stem cell inducing cardiomyocytes is cultivated and measures bio-reactor cut-away view.
Embodiment
Below in conjunction with accompanying drawing, the utility model is described in further detail.
The utility model controls steering wheel by unicircuit and regulates nutrient solution flow differentiation of stem cells cardioblast, and beat intensity and the frequency of myocardial cell is detected by culturing room's base pressure sensor, design and develop a set of bio-reactor measured with pulsating force about the cultivation of organizational project stem cell inducing cardiomyocytes.As shown in Figure 1, a kind of organizational project stem cell inducing cardiomyocytes is cultivated and is mainly contained cultivation unit, control unit with measurement bio-reactor, and measuring unit part forms.
Cultivate unit and comprise the second drain pipe 39; First drain pipe 40; 3rd jointing 41; First jointing 42; Container for storing liquid firm banking 43; First container for storing liquid 44; Second jointing 45; First nutrient solution container for storing liquid cover plate 1; Second nutrient solution container for storing liquid 2; Second nutrient solution container for storing liquid cover plate 3; 3rd nutrient solution container for storing liquid cover plate 4; 4th nutrient solution container for storing liquid cover plate 5; 3rd nutrient solution container for storing liquid 6; 4th nutrient solution container for storing liquid 7; 4th jointing 8; 3rd drain pipe 9; 4th drain pipe 10; 8th drain pipe 17; 7th drain pipe 18; Culturing room's cover plate 19; Culturing room 20; Culturing room's waste liquid outlet jointing 21; Waste liquid by-pass valve control 22; 9th drain pipe 23; Waste liquid container for storing liquid jointing 24; Waste liquid tank cover plate 27; Waste liquid tank base 28; 6th drain pipe 29; 5th drain pipe 30; 6th jointing 48; 7th jointing 49; 8th jointing 50; 5th jointing 52.
Four container for storing liquids (44,2,6,7) are contained on container for storing liquid firm banking 43, connect corresponding drain pipe (40,39,9,10) by respective jointing (42,45,41,8); Culturing room 20 is on waste liquid tank base 28, and the left side connects four drain pipes (30,29,18,17), and nutrient solution enters thus, and the right connects the 9th drain pipe 23, and waste liquid is discharged to waste liquid pool.
As shown in figures 1 and 3, control unit comprises the first steering wheel 11; 3rd steering wheel 12; Second steering wheel 13; 4th steering wheel 14; Steering wheel retaining plate set screw 15; Front apron 16; Front apron set screw 31; Steering wheel retaining plate 32; Steering wheel connects string holes 33; Nutrient solution Flow-rate adjustment base 34; Steering wheel set screw 35; Surface-mounted integrated circuit firm banking 36; Base plate set screw 37; Rear deflector door 38; 3rd Flow-rate adjustment cam 46; 4th Flow-rate adjustment cam 47; Second flow adjustment cam 53; First flow adjustment cam 54; Rear deflector door set screw 55; Surface-mounted integrated circuit 56; Board support isolating joint 57.
Surface-mounted integrated circuit 56 is fixed on surface-mounted integrated circuit firm banking 36 by rear deflector door set screw 55 and board support isolating joint 57; Nutrient solution Flow-rate adjustment base 34 is arranged on on circuit card firm banking 36, and the left side connects four drain pipes (40,39,9,10), and the right connects four drain pipes (30,29,18,17), and steering wheel retaining plate 32 is housed above; Four steering wheels (11,13,12,14) are arranged on steering wheel retaining plate 32, connect corresponding four Flow-rate adjustment cams (54,53,46,47) below.
As illustrated in fig. 1 and 2, measuring unit comprises culturing room's sensor and fixes barrier film 25; Sensor fixation plate 26; Piezoelectric transducer 51; Piezoelectric transducer 51 is fixed barrier film 25 by sensor and is fixed on sensor fixation plate 26.
The effect of cultivation unit is the myocardial cell needed for cultivation; Nutrient solution container for storing liquid has four, can store four kinds of different nutrient solutions; The uninterrupted of input nutrient solution can regulate as requested; The waste liquid of discharging through culturing room is reclaimed by waste liquid pool; Whole myocardial cells culture unit is sealing, avoids the impact of bacterium in contact outside air.
Organizational project myocardial cell control unit mainly contains steering wheel, Flow-rate adjustment cam, the composition such as surface-mounted integrated circuit and fixing link.
Unicircuit external touch, can manual parameters on the touchscreen, is controlled the action of steering wheel, and then drives the action of Flow-rate adjustment cam, finally realize the adjustment to nutrient solution flow by unicircuit; Fixing link realizes the fixed action to various piece.
Organizational project myocardial cell measuring unit mainly contains piezoelectric transducer, and sensor fixes barrier film and sensor fixation plate composition.
The sensor barrier film that is fixed is fixed on retaining plate, and sensor will record myocardial cell's intensity of beating and be transformed into voltage signal and output in external touch, carry out real-time curve display.
The process using said apparatus to carry out the cultivation of organizational project stem cell inducing cardiomyocytes and measurement is:
1, in sterile environment, close waste liquid by-pass valve control, in nutrient solution container for storing liquid, load required nutrient solution, external touch inputs initial value, and the nutrient solution of servos control certain flow enters culturing room.
2, open culturing room's cover plate in an aseptic environment, myocardial cell suspensions is instilled culturing room, cover culturing room's cover plate, system is put into incubator and cultivates.
3, in cultivation Induction Process, intensity curve that observation external touch is beaten, regulates different sorts nutrient solution flow control parameter as required, can obtain the intensity of beating of myocardial cell according to curve of beating, and calculates can obtain Beating Rate further through control software design.
4, in cultivation Induction Process, can open waste liquid by-pass valve control at any time, get rid of waste liquid or unnecessary nutrient solution, can control any Flow-rate adjustment cam puts into the inducing culture that new nutrient solution carries out myocardial cell simultaneously, after cultivation completes, collect myocardial cell and carry out various follow-up work.
Claims (1)
1. organizational project stem cell inducing cardiomyocytes is cultivated and is measured a bio-reactor, comprises and cultivates unit, control unit and measuring unit, it is characterized in that:
Described cultivation unit comprises multiple container for storing liquid and a culturing room, and each container for storing liquid connects corresponding drain pipe by respective jointing, and nutrient solution is inputed to control unit; Control unit controls the nutrient solution entering culturing room; Waste liquid tank is provided with, culturing room and waste liquid tank UNICOM below described culturing room;
Described control unit comprises Flow-rate adjustment cam and drives the steering wheel of Flow-rate adjustment cam; The flow inputing to each road nutrient solution of control unit is controlled by Flow-rate adjustment cam, and steering wheel is arranged on the top of Flow-rate adjustment cam, and steering wheel is controlled by surface-mounted integrated circuit, and surface-mounted integrated circuit is circumscribed with touch-screen;
Described measuring unit comprises culturing room's sensor and fixes barrier film, sensor fixation plate and piezoelectric transducer; Piezoelectric transducer is fixed barrier film by sensor and is fixed on sensor fixation plate, and sensor fixation plate is between culturing room and waste liquid tank, and piezoelectric transducer is used for beating of perception myocardial cell.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420700163.6U CN204311067U (en) | 2014-11-20 | 2014-11-20 | A kind of organizational project stem cell inducing cardiomyocytes is cultivated and is measured bio-reactor |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420700163.6U CN204311067U (en) | 2014-11-20 | 2014-11-20 | A kind of organizational project stem cell inducing cardiomyocytes is cultivated and is measured bio-reactor |
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| Publication Number | Publication Date |
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| CN204311067U true CN204311067U (en) | 2015-05-06 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201420700163.6U Expired - Fee Related CN204311067U (en) | 2014-11-20 | 2014-11-20 | A kind of organizational project stem cell inducing cardiomyocytes is cultivated and is measured bio-reactor |
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| Country | Link |
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| CN (1) | CN204311067U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104388311A (en) * | 2014-11-20 | 2015-03-04 | 杭州电子科技大学 | Bioreactor for culturing and measuring tissue engineering stem cell-induced myocardial cells |
| CN116286315A (en) * | 2023-03-21 | 2023-06-23 | 南京鼓楼医院 | Device and method for detecting pulsation dynamic pressure of tissue engineering myocardial cells |
-
2014
- 2014-11-20 CN CN201420700163.6U patent/CN204311067U/en not_active Expired - Fee Related
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104388311A (en) * | 2014-11-20 | 2015-03-04 | 杭州电子科技大学 | Bioreactor for culturing and measuring tissue engineering stem cell-induced myocardial cells |
| CN104388311B (en) * | 2014-11-20 | 2016-09-21 | 杭州电子科技大学 | Organizational project stem cell inducing cardiomyocytes is cultivated and measures bioreactor |
| CN116286315A (en) * | 2023-03-21 | 2023-06-23 | 南京鼓楼医院 | Device and method for detecting pulsation dynamic pressure of tissue engineering myocardial cells |
| CN116286315B (en) * | 2023-03-21 | 2023-10-27 | 南京鼓楼医院 | Device and method for detecting pulsation dynamic pressure of tissue engineering myocardial cells |
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| Date | Code | Title | Description |
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20150506 Termination date: 20181120 |
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| CF01 | Termination of patent right due to non-payment of annual fee |