CN205062055U - Phytoplankton reation kettle and adopt this reation kettle's continuous culture to put permanent disguise - Google Patents
Phytoplankton reation kettle and adopt this reation kettle's continuous culture to put permanent disguise Download PDFInfo
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- CN205062055U CN205062055U CN201520781096.XU CN201520781096U CN205062055U CN 205062055 U CN205062055 U CN 205062055U CN 201520781096 U CN201520781096 U CN 201520781096U CN 205062055 U CN205062055 U CN 205062055U
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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Abstract
The utility model relates to a phytoplankton continuous culture puts permanent the disguise, including reation kettle, temperature control unit, nutrient solution supply unit, nutrient solution discharge unit, gaseous supply unit, sampling unit, magnetic stirrers unit and illumination the control unit. Reation kettle includes the cauldron body and kettle cover, the cauldron body sets up water inlet and delivery port for bilayer structure on the skin, the kettle cover top sets up kettle cover passageway I, kettle cover passageway II, kettle cover passageway III and inoculation opening, set up the nutrient solution pipe in the kettle cover passageway I, set up the three -way cock valve on the kettle cover passageway II, three -way cock valve intercommunication kettle cover passageway II, sampling outlet and air inlet, set up the nutrient solution discharger in the kettle cover passageway III. This novel stable state chemostat device through totally closed operation, can realize that phytoplankton's continuous stable state is cultivateed, has fabulous repeatability, stability and environmental parameter controllability, and its sample process is pollution -free, simple, and noiseless to phytoplankton steady -state growth.
Description
Technical field
The utility model relates to the culture apparatus of a kind of plant plankton, is specifically related to a kind of permanent gasifying device of novel stable state can carrying out cultured continuously for plant plankton.
Background technology
Chemostat cultivation refers to and carry out microorganism culturing or cell cultures in the system of condition relative constancy, and the nutritive medium in this system remains unchanged, and is supplied with constant rate of speed by the external world, and extracts culture continuously; This culture growth velocity is come accurately to control by the delivery rate limiting certain nutritive ingredient, and this method not only can improve plant factor, is also conducive to the culture experiment of carrying out high-precision requirement.
At present, there is following technical problem in the chemostat cultivation device for marine phytoplankton: (1) poor stability, poor repeatability: cultured continuously experimental installation was often difficult to accurately control phytoplankton growth condition fully in the past, thus be difficult to control Phytoplankton Cells growth velocity, cause pattern of cell growth unstable; And poor repeatability between different experiments, be difficult to repeated authentication experimental result.It is in sampling process, easily produces disturbance to phytoplankton growth balance, causes plant plankton to be difficult to remain on steady state.(2) easily pollute: because cultured continuously experiment is not in the past often totally-enclosed environment, this makes the plant plankton in system easily be subject to the interference of external microbe and bacterium etc., after causing cultivating, Phytoplankton Cells, bacterium or other microorganisms coexist, for further to analyze and test brings difficulty.And owing to polluting interference, experimental result is also inaccurate, reduces laboratory reference and be worth.(3) pollution-free and accurately sample difficulty: in the past sampling operation taked often to extract or cultivate after disposable sampling etc. mode.These modes are not best, because the easy continuous steady-state growth state of disturbance plant plankton, and easily bring pollution.If stop cultivation sampling, the growth balance of plant plankton also can be interrupted.If cultivate and terminate rear sampling, be so just difficult to real-time monitored to plant plankton Parameters variation in process of growth, as cell count, cellular constituent change, Growth of Cells mechanism and Parameters variation etc.(4) environmental parameter is difficult to accurate analog and control: this is mainly manifested in simulation to nutritive medium, temperature, illumination etc. environmental parameter and control.Allied equipment in the past, often only can simulate and control wherein one or two condition, and under being difficult to more than meeting multiple conditions such as " pollution-free samplings, stablize stable state, reproducible ", realizes accurate analog and the control of full environmental parameter.
Utility model content
The utility model aims to provide a kind of novel plant plankton reactor and adopts the permanent gasifying device of the cultured continuously of this reactor, the problems such as existing chemostat cultivation device stability is poor to solve, poor repeatability, sampling is difficult, multivariable control is difficult, easy pollution.
The technical scheme that the utility model is taked is as follows:
A kind of plant plankton reactor, comprise kettle and kettle cover, described kettle is bilayer structure, skin sets into the mouth of a river and water outlet, described kettle cover top arranges kettle cover passage I, kettle cover passage II, kettle cover passage III and inoculation mouth, in described kettle cover passage I, the nutritive medium conduit extending to kettle is set, kettle cover passage II is arranged three-way tap valve, three-way tap valve is communicated with kettle cover passage II, sampling outlet and gas inlet, the thrust-augmenting nozzle extending to kettle is set in kettle cover passage II, in described kettle cover passage III, the nutritive medium discharger extending to kettle is set.
The lower end of described nutritive medium conduit is positioned at more than the liquid level of predetermined nutrient solution, the lower end of nutritive medium discharger is positioned at the liquid level top layer of predetermined nutrient solution, with liquid level close contact, the lower end of thrust-augmenting nozzle (simultaneously also as the airway of input gas) is positioned at below the liquid level of predetermined nutrient solution.
The distance of described water inlet and water outlet is more far away better, can ensure the adequacy of water cycle in interlayer.
The making material preferably transparent material of described reactor, as glass.
The interior space volume of described reactor is 3L (can be used for low growth velocity plant plankton) or 5.0L (can be used for high growth rates plant plankton), is used as the growth room of plant plankton.
Described inoculation mouth is added a cover at ordinary times or is used diaphragm of rubber to seal, and opens lid or direct syringe stings diaphragm of rubber when needing, and inoculates plant plankton with syringe.
Adopt the permanent gasifying device of the cultured continuously of above-mentioned plant plankton reactor, comprise reactor, temperature control unit, nutritive medium feed unit, nutritive medium deliverying unit, gas feed unit, sampling unit, magnetic stirring apparatus unit and lighting control unit; Water inlet and the water outlet of described temperature control unit and reactor are connected; The nutritive medium tubes connection of described nutritive medium feed unit and reactor; Described nutritive medium deliverying unit is connected with the nutritive medium discharger of reactor; Described gas feed unit is connected with the gas inlet of reactor; Described sampling unit is connected with the sampling outlet of reactor; Described magnetic stirring apparatus unit comprises magnetic stir bar and magnetic stirring apparatus, and magnetic stir bar is placed on the inside of reactor, and magnetic stirring apparatus is placed on the below of reactor; Described lighting control unit is arranged on the side of reactor, and light directly shines into reactor.
Described temperature control unit comprises recirculated water bath, and recirculated water bath is connected by the water inlet of pipeline and reactor and water outlet.
Described nutritive medium feed unit comprises nutritive medium reserve room and peristaltic pump, and nutritive medium reserve room is connected with peristaltic pump by pipeline, and peristaltic pump is again by the nutritive medium tubes connection of pipeline and reactor.
Described nutritive medium deliverying unit comprises spillage storage bottle and flexible pipe, and one end of the nutritive medium discharger connecting hose of reactor, the other end of flexible pipe is placed in spillage storage bottle.
Described gas feed unit comprises aerator pump and filter sieve, and aerator pump is connected with one end of filter sieve by flexible pipe, and the other end of filter sieve is connected with the gas inlet of reactor.The preferred aperture of filter sieve is 0.22 μm, can impurity screening and microorganism etc., ensures the injection of sterile air.
Described sampling unit comprises syringe and Erlenmeyer flask, and syringe and Erlenmeyer flask are by hose connection, and Erlenmeyer flask top is connected with the sampling outlet of reactor by flexible pipe.
Described lighting control unit comprises optic panel, fluorescent tube, timer and photon meter, and fluorescent tube is arranged on optic panel, and fluorescent tube connects timer, and timer connects power supply; Fluorescent tube and optic panel provide and reflected light shines; Timer Controlling light application time, can be set to Continuous irradiation, or day alternates with night irradiate; Photon meter connects computer, for detecting light intensity at any time, guarantees that Optical power values is accurate.
Described permanent gasifying device also comprises support unit, and described support unit comprises bracing frame, and bracing frame arranges binding clip, and binding clip is clipped in the joint of kettle and kettle cover.
For the several shortcomings existed in prior art, the utility model mainly solves by the following technical programs:
(1) for the shortcoming of " poor stability, poor repeatability ": the utility model takes four countermeasure: a. strictly to control nutrient infusion speed by peristaltic pump, b. accurately growth temperature is controlled by double-jacket and water bath with thermostatic control, c. by the strict controlled light intensity of intensity control instrument, d. liquid flow velocity in reactor is controlled by magnetic stirring apparatus, thus accurate analog control phytoplankton growth environment, realize stable growth environment.The utility model good stability, has splendid repeatability.
(2) for the shortcoming of " easily polluting ": the utility model adopts totally-enclosed space, pollution-free experiment flow.The support equipment of whole reactor device and connection is all in free of contamination enclosed space, nutritive medium and the air sieved filter all after filtration passed into, to reject environmental microorganism impact, so the interference of extraneous bacterium and virus can be got rid of on the whole, thus realize pollution-free experiment flow, be conducive to carrying out cultured continuously to single algae kind or many algaes kind.The injection of nutritive medium and gas and discharge process are one-way fashion, without backflow, can not cause backflow pollution.Especially nutritive medium adopts micro-pressure difference method continuous micro to discharge, and avoids artificial disturbance.And in magnetic stirring apparatus, external stirrer, but built-in stirrer, both are separated, thus avoid to need to pass into and stir power set to the potentially contaminated driving stirrer to bring in the past.In a word, no matter cultivate the sampling in front beamhouse operation, cultivation and the analysis after cultivating, all under aseptic conditions, realize omnidistance pollution-free.
(3) for the shortcoming of " pollution-free accurate sampling is difficult ": utilize three-way tap valve, change gas passage, utilize the pressure produced in reactor inside, liquid in reactor is initiatively discharged.Because gas is in advance through filter-sterilized, so whole process not contact reacts still internal liquid, thus realize pollution-free sampling.And, due to phytoplankton growth undisturbed, real time sample any time can be realized, thus the whole process that Phytoplankton Cells grows can be observed.
(4) for the shortcoming of " environmental parameter is difficult to accurate analog and control ": the utility model adopts multinomial new design to solve.Show: 1. at nutritive medium supply respect: use peristaltic pump accurately to control nutritive medium feed rate and speed, simultaneously, by air pressure elementary errors, remaining culture liq is discharged, with guarantee when pollution-free, without artificial interference, keep the steady state conditions of phytoplankton growth; 2. in temperature control: transparent glass double-jacket is arranged, accurate temperature controlling is realized by circulator bath, error only ± 0.1 DEG C, thus guarantee not only can set any precise constant temperature in growth room, and temperature can be changed at any time to study the impact of temperature variation on plant plankton; 3. in intensity of illumination control: by controllability light intensity plate, and detect light intensity at any time by precision instrument, thus the accurate control to light intensity is realized; And light intensity irradiation time is by timer, can change at any time, can realize the various modes such as Continuous irradiation, diurnal cycle irradiation.4. water body flow is simulated: carry out prolonged agitation by magnetic stirring apparatus, the water wave in simulated environment and fluctuation.In a word, all envrionment conditionss, all can be controlled accurately and be simulated in the utility model.
In sum, relative to prior art, the beneficial effects of the utility model are embodied in:
(1) perfect steady-state simulation culture apparatus, good stability, reproducible: the utility model ideally simulates phytoplankton growth environment, realize the environmental parameters such as temperature, light intensity, nutrient solution and physical perturbation all controlled, guarantee Phytoplankton Cells can under steady state conditions, a reactor continuously, stable growth; Guarantee that sampling process is to phytoplankton growth undisturbed, pollution-free; Guarantee to carry out the on all four repeated experiment of experiment condition.
(2) enclosed space, pollution-free experiment: whole reactor device is in free of contamination enclosed space, nutritive medium and the gas that passes into all through filter-sterilized, so the interference of extraneous bacterium and virus can be got rid of; Adopt the sampling of gas differential pressure method, eliminate artifact pollution; Magnetic stirring apparatus is placed on bottom reactor, and stirrer is placed on reactor inside, avoids disturbance and pollute; More than design achieves pollution-free experiment flow, is conducive to carrying out cultured continuously to plant plankton.
(3) nutritive medium supply and discharging: the method adopting micro-pressure difference, the liquid continuous stable one direction injecting reactor is discharged, and there will not be refluence and outside contamination, can not phytoplankton growth balance in disturbance reactor.
(4) pollution-free sampling: utilize three-way tap valve, changes gas passage, utilizes the pressure produced in reactor inside, by Liquid extracting in reactor.Because gas is in advance through filter-sterilized, so whole process not contact reacts still internal liquid, internal liquid uniflux, without backflow pollution, thus realizes pollution-free sampling.
(5) accurately nutrient infusion is controlled: the input rate that accurately can be controlled nutritive medium by peristaltic pump, thus the growth velocity of plant plankton can be controlled, nutritive salt (as nitrate, phosphoric acid salt and silicate etc.) restriction and Control release can be carried out.
(6) precise control of temperature: by reacting kettle jacketing and circulator bath, realizes the accurate control to temperature.And due to reactor itself be transparent, this temperature control process can't have an impact to illumination, thus at one stroke two.
(7) accurately light intensity and illumination mode is controlled: because whole reactor is transparent, by the extraneous light intensity of adjustment reactor wall, phytoplankton growth speed can be controlled, thus light restriction experiment can be carried out, realize stable state under light restricted condition and cultivate or cultured continuously round the clock; Also different light pattern and light intensity can be studied on the impact of phytoplankton growth by adjustment intensity of illumination and light application time.
(8) undisturbed cultured continuously: after each sampling, kettle inside will be full of by the nutritive medium be newly filled with again, the nutrient solution be newly filled with is to be similar to the algae in the overflow manner dilution cultivation of stable situation, algae can regrow at short notice to steady-state growth rate, by strictly calculating and control sampling amount and sample time, substantially disturbance and impact can not be produced on the steady-state growth of plant plankton.
Accompanying drawing explanation
Fig. 1 the utility model plant plankton reactor structural representation;
The permanent gasifying device structural representation of Fig. 2 the utility model plant plankton cultured continuously;
Fig. 3 the utility model lighting control unit structural representation.
In figure: 1-sampling outlet, 2-nutritive medium conduit, 3-kettle cover passage I, 4-kettle cover, 5-kettle, 6-water outlet, 7-gas inlet, 8-three-way tap valve, 9-nutritive medium discharger, 10-kettle cover passage II, 11-kettle cover passage III, 12-inoculates mouth, 13-water inlet, 14-syringe, 15-Erlenmeyer flask, 16-nutritive medium reserve room, 17-peristaltic pump, 18-filter sieve, 19-aerator pump, 20-spillage storage bottle, 21-recirculated water bath, 22-magnetic stir bar, 23-reactor support, 24-magnetic stirring apparatus, 25-liquid level, 26-thrust-augmenting nozzle, 27-optic panel, 28-fluorescent tube, 29-timer, 30-power supply.
Embodiment
Below in conjunction with specific embodiment and accompanying drawing, the utility model is described in further details.
Plant plankton reactor as shown in Figure 1, comprises kettle 5 and kettle cover 4.Kettle 5 is bilayer structure, skin sets into the mouth of a river 13 and water outlet 6, and water inlet 13 and water outlet 6 lay respectively at the upper and lower of kettle 5, distant, ensures the adequacy of water cycle.Kettle cover 4 top arranges kettle cover passage I 3, kettle cover passage II 10, kettle cover passage III 11 and inoculation mouth 12.The nutritive medium conduit 2 extending to kettle 5 is set in kettle cover passage I 3; Kettle cover passage II 10 is arranged three-way tap valve 8, three-way tap valve 8 is communicated with kettle cover passage II 10, sampling outlet 1 and gas inlet 7, arranges the thrust-augmenting nozzle 26 extending to kettle 5 in kettle cover passage II 10; The nutritive medium discharger 9 extending to kettle 5 is set in kettle cover passage III 11.The making material of reactor is glass, conveniently can observe the growth conditions of plant plankton on the one hand, and the light also not affecting outside lighting control unit is on the other hand injected.
The lower end of described nutritive medium conduit 2 is positioned at the liquid level more than 25 of predetermined nutrient solution, and the lower end of thrust-augmenting nozzle 26 is positioned at the liquid level less than 25 of predetermined nutrient solution, and the lower end of nutritive medium discharger 9 is positioned at the liquid level top layer of predetermined nutrient solution, with liquid level close contact.
The permanent gasifying device of cultured continuously as shown in Figure 2, comprises above-mentioned plant plankton reactor, temperature control unit, nutritive medium feed unit, nutritive medium deliverying unit, gas feed unit, sampling unit, magnetic stirring apparatus unit and lighting control unit.
Temperature control unit comprises recirculated water bath 21, and recirculated water bath 21 is connected by the water inlet 13 of flexible pipe and reactor and water outlet 6.Recirculated water bath 21 is delivered in the interlayer of kettle 5, to control the specified temp of reactor temperature needed for plant plankton, error ± 0.1 DEG C by heating (or cooling) water to plant plankton optimum growth temp continuously.
Nutritive medium feed unit comprises nutritive medium reserve room 16 and peristaltic pump 17, and nutritive medium reserve room 16 is connected with peristaltic pump 17 by flexible pipe, and peristaltic pump 17 is connected with the nutritive medium conduit 2 of reactor by pipeline again.
Nutritive medium deliverying unit comprises spillage storage bottle 20 and flexible pipe, and one end of nutritive medium discharger 9 connecting hose of reactor, the other end of flexible pipe is placed in spillage storage bottle 20.
Gas feed unit comprises aerator pump 19 and filter sieve 18, and aerator pump 19 is connected by flexible pipe one end with filter sieve 18, and the other end of filter sieve 18 is connected with the gas inlet 7 of reactor.
Sampling unit comprises syringe 14 and Erlenmeyer flask 15, and syringe 14 and Erlenmeyer flask 15 are by hose connection, and Erlenmeyer flask 15 is connected with the sampling outlet 1 of reactor by flexible pipe.
Magnetic stirring apparatus unit comprises magnetic stir bar 22 and magnetic stirring apparatus 24, magnetic stir bar 22 is placed on the inside of reactor, magnetic stirring apparatus 24 is placed on the below of reactor, between reactor and magnetic stirring apparatus 24, reactor support 23 is also set, reactor is stably placed on magnetic stirring apparatus 24.
Lighting control unit is arranged on the side of reactor, and light directly shines into reactor.As shown in Figure 3, comprise optic panel 27, fluorescent tube 28, timer 29 and photon meter, fluorescent tube 28 is arranged on optic panel 27, and fluorescent tube 28 connects timer 29, and timer 29 connects power supply, and photon meter is placed in reactor side, and connects computer.
Described permanent gasifying device also comprises support unit, and described support unit comprises bracing frame, and bracing frame arranges binding clip, and binding clip is clipped in the joint of kettle 5 and kettle cover 4.
The permanent gasifying device of above-mentioned plant plankton cultured continuously before use, first by good for each for reactor assembling parts, then assemble in order with nutritive medium deliverying unit, gas feed unit (except aerator pump), sampling unit and magnetic stir bar, then carry out sterilising treatment together.Plant plankton nutrient solution degerming is after filtration injected nutritive medium reserve room 16, it is inner that nutrient solution is added dropwise to kettle 5 constantly through peristaltic pump 17, when nutrient solution height reaches predetermined level 25, open temp control unit, external magnetic stirring apparatus, lighting control unit and gas feed unit.When homo(io)thermism, system stability, by inoculating the film of mouth 12, with the plant plankton of syringe inoculation for cultivating.Wherein the mode of operation of each unit is as follows: temperature control unit is by recirculated water bath 21 pairs of water heating (or cooling), water after heating (or cooling) enters kettle 5 water inlet 13 through flexible pipe, fully flows out through water outlet 6 and returns recirculated water bath 21 after circulation and again heat (or cooling) and carry out constant temperature in interlayer.Gas feed unit is by the aerator pump 19 of speed control inputting sterile air, sterile air is after three-way tap valve 8 switch, import reactor, the position close to bottom is imported again through thrust-augmenting nozzle 26, gas overflows from bottom to upper strata in the form of bubbles, thus supplements carbonic acid gas for the growth of plant plankton.It is inner that nutrient solution in nutritive medium reserve room 16 is continued instillation kettle 5 through flexible pipe by peristaltic pump 17 by nutritive medium feed unit.The lower end of the nutritive medium discharger 9 of nutritive medium deliverying unit is positioned at the liquid level top layer of predetermined nutrient solution, with liquid level close contact, because aseptic gas continues to be filled with, makes sheaf space on reactor nutrient solution keep certain steady pressure; Along with the continuous instillation of nutritive medium, internal pressure increases a little, and can produce slight pressure difference between ambient pressure, and this pressure difference can force unnecessary nutrient solution to continuously outflow through nutritive medium discharger 9.Magnetic stirring apparatus 24 in magnetic stirring apparatus unit provides power for magnetic stir bar 22, and the latter is placed in reactor, can stir the nutrient solution in reactor, thus makes simulation drive marine condition, and nutrient solution in reactor is mixed all the time.Sampling unit by controlling three-way tap valve 8, and extracts realization with syringe 14; Before sampling, change gas passage by three-way tap valve 8, in outside with syringe 14 extracting liquid, sample, because whole process is liquid one way flow, the pollution of sampling process can be reduced like this.In whole culturing process, keep the running of peristaltic pump 17; Interference effect to cultured continuously stable state during for reducing sampling, the volume of every sub-sampling must not exceed the nutrient solution volume be filled with during two sampling spots in kettle 5, and must not exceed kettle 5 cumulative volume ~ 6%.The fluorescent tube 28 of lighting control unit and optic panel 27 provide and reflected light photograph, and can control light intensity; The timer 29 controlled light time, can Continuous irradiation be set to, or day alternates with night irradiate; Photon meter is used for detecting light intensity at any time, guarantees that Optical power values is accurate.
Claims (10)
1. a plant plankton reactor, it is characterized in that, comprise kettle (5) and kettle cover (4), described kettle (5) is bilayer structure, skin sets into the mouth of a river (13) and water outlet (6), described kettle cover (4) top arranges kettle cover passage I (3), kettle cover passage II (10), kettle cover passage III (11) and inoculation mouth (12), in described kettle cover passage I (3), the nutritive medium conduit (2) extending to kettle (5) is set, kettle cover passage II (10) is arranged three-way tap valve (8), three-way tap valve (8) is communicated with kettle cover passage II (10), sampling outlet (1) and gas inlet (7), the thrust-augmenting nozzle (26) extending to kettle (5) is set in kettle cover passage II (10), in described kettle cover passage III (11), the nutritive medium discharger (9) extending to kettle (5) is set.
2. plant plankton reactor according to claim 1, is characterized in that, the making material of described reactor is transparent material.
3. one kind adopts the permanent gasifying device of the cultured continuously of plant plankton reactor described in claim 1 or 2, it is characterized in that, comprise reactor, temperature control unit, nutritive medium feed unit, nutritive medium deliverying unit, gas feed unit, sampling unit, magnetic stirring apparatus unit and lighting control unit; Water inlet (13) and the water outlet (6) of described temperature control unit and reactor are connected; Described nutritive medium feed unit is connected with the nutritive medium conduit (2) of reactor; Described nutritive medium deliverying unit is connected with the nutritive medium discharger (9) of reactor; Described gas feed unit is connected with the gas inlet (7) of reactor; Described sampling unit is connected with the sampling outlet (1) of reactor; Described magnetic stirring apparatus unit comprises magnetic stir bar (22) and magnetic stirring apparatus (24), and magnetic stir bar (22) is placed on the inside of reactor, and magnetic stirring apparatus (24) is placed on the below of reactor; Described lighting control unit is arranged on the side of reactor, and light directly shines into reactor.
4. the permanent gasifying device of plant plankton cultured continuously according to claim 3, it is characterized in that, described temperature control unit comprises recirculated water bath (21), and recirculated water bath (21) is connected by the water inlet (13) of pipeline and reactor and water outlet (6).
5. the permanent gasifying device of plant plankton cultured continuously according to claim 3, it is characterized in that, described nutritive medium feed unit comprises nutritive medium reserve room (16) and peristaltic pump (17), nutritive medium reserve room (16) is connected with peristaltic pump (17) by pipeline, and peristaltic pump (17) is connected with the nutritive medium conduit (2) of reactor by pipeline again.
6. the permanent gasifying device of plant plankton cultured continuously according to claim 3, it is characterized in that, described nutritive medium deliverying unit comprises spillage storage bottle (20) and flexible pipe, one end of nutritive medium discharger (9) connecting hose of reactor, the other end of flexible pipe is placed in spillage storage bottle (20).
7. the permanent gasifying device of plant plankton cultured continuously according to claim 3, it is characterized in that, described gas feed unit comprises aerator pump (19) and filter sieve (18), aerator pump (19) is connected by flexible pipe one end with filter sieve (18), and the other end of filter sieve (18) is connected with the gas inlet (7) of reactor.
8. the permanent gasifying device of plant plankton cultured continuously according to claim 3, it is characterized in that, described sampling unit comprises syringe (14) and Erlenmeyer flask (15), syringe (14) and Erlenmeyer flask (15) are by hose connection, and Erlenmeyer flask (15) top is connected with the sampling outlet (1) of reactor by flexible pipe.
9. the permanent gasifying device of plant plankton cultured continuously according to claim 3, it is characterized in that, described lighting control unit comprises optic panel (27), fluorescent tube (28), timer (29) and photon meter, fluorescent tube (28) is arranged on optic panel (27), fluorescent tube (28) connects timer (29), timer (29) connects power supply (30), and photon meter connects computer.
10. the permanent gasifying device of plant plankton cultured continuously according to claim 3, it is characterized in that, described permanent gasifying device also comprises support unit, described support unit comprises bracing frame, bracing frame arranges binding clip, and binding clip is clipped in the joint of kettle (5) and kettle cover (4).
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107513496A (en) * | 2016-06-17 | 2017-12-26 | 上海市农药研究所有限公司 | Unicellular alga auto culturing system and its application |
| CN109142549A (en) * | 2018-08-27 | 2019-01-04 | 睿藻生物科技（苏州）有限公司 | A kind of microalgae metabolic rate measurement chamber for mass spectrometer |
| CN110408517A (en) * | 2019-08-05 | 2019-11-05 | 南开大学 | Natural fresh water plankton microcosm system construction device and method |
| CN110663537A (en) * | 2019-11-18 | 2020-01-10 | 郭俊辉 | Phytoplankton cultivates feeder |
| CN114608648A (en) * | 2022-02-16 | 2022-06-10 | 中国科学院南海海洋研究所 | Medium universe culture device for simulating influence of rainfall on plankton and greenhouse gas |
-
2015
- 2015-10-10 CN CN201520781096.XU patent/CN205062055U/en active Active
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107513496A (en) * | 2016-06-17 | 2017-12-26 | 上海市农药研究所有限公司 | Unicellular alga auto culturing system and its application |
| CN109142549A (en) * | 2018-08-27 | 2019-01-04 | 睿藻生物科技（苏州）有限公司 | A kind of microalgae metabolic rate measurement chamber for mass spectrometer |
| CN110408517A (en) * | 2019-08-05 | 2019-11-05 | 南开大学 | Natural fresh water plankton microcosm system construction device and method |
| CN110663537A (en) * | 2019-11-18 | 2020-01-10 | 郭俊辉 | Phytoplankton cultivates feeder |
| CN114608648A (en) * | 2022-02-16 | 2022-06-10 | 中国科学院南海海洋研究所 | Medium universe culture device for simulating influence of rainfall on plankton and greenhouse gas |
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