CN114608648A - Medium universe culture device for simulating influence of rainfall on plankton and greenhouse gas - Google Patents

Abstract

The invention discloses a mesocosm culturing device for simulating the effect of rainfall on plankton and greenhouse gases, belonging to the field of ecological environment effects during rainfall, comprising: a base culture tank and a top cover, wherein the base culture tank is closed at the bottom A tank body with an open upper end, the side wall of the tank body is provided with a circulating pump, and the circulating pump is used to promote the circulation exchange of the water body in the base culture tank; the top cover is arranged on the base culture tank, The top cover is provided with a light-emitting device and a sprayer, the light-emitting device is used for simulating sunlight irradiation, and the sprayer is used for simulating rainfall. The invention can provide important scientific support for the research on the ecological effect of plankton changes caused by rainfall and the climatic effect affecting the release of main greenhouse gases.

Description

A Mesocosmic Culture Device to Simulate the Effects of Rainfall on Plankton and Greenhouse Gases

technical field

The invention relates to the field of ecological environment effects belonging to the rainfall process, in particular to a mesocosmic cultivation device for simulating the impact of rainfall on plankton and greenhouse gases.

Background technique

my country has become one of the world's main deposition areas of atmospheric nutrients (mainly nitrogen), and with the rapid development of industrialization and the continuous strengthening of human activities, the deposition of atmospheric nutrients presents a trend of increasing. Rainfall is the main way of nutrient deposition, especially in the southern regions where there is more rainfall and more lakes and rivers in my country, as well as coastal and marginal sea areas. Nutrients carried by rainfall input will have a serious impact on terrestrial and marine ecosystems. For example, the input of nitrogen deposition carried by rainfall will make the forest ecosystem canopy thin, reduce the ability of forests to resist pests and diseases, and may even cause forests to gradually increase over time. It can also significantly change the species composition of the lichen community, that is, from a highly nitrogen-sensitive community to a nitrogen-tolerant community; affect the composition of soil microbial diversity (bacteria, fungi, actinomycetes, etc.), and reduce the mycorrhizal root system. Expansion capacity, fungal fruiting body yield and species richness.

Nutrient input pairs carried by rainfall are an important source of nutrients for terrestrial rivers, lakes, and oceans. At present, there are reports on on-site observation of rainfall and its impact on the community structure of marine phytoplankton. However, while rainfall affects plankton changes, the impact of rainfall on _CO2 caused by changes in plankton respiration process and on nitrifying and denitrifying bacteria are also reported. However, the effect on N ₂ O release, and whether changes in the bacterial community structure such as Methanogens and Methanococcus in water would affect the release of CH ₄ are still less reported.

SUMMARY OF THE INVENTION

In view of the deficiencies in the prior art, the present invention provides a mesocosmic cultivation device for simulating the effects of rainfall on plankton and greenhouse gases. Gas-related bacterial activity is simultaneously observed and how rainfall processes affect key greenhouse gas emission processes. At the same time, while determining the ecological effects of rainfall on water bodies, observing the impact of rainfall on the production process of major greenhouse gases can provide important scientific support for the study of the ecological effects of plankton changes caused by rainfall and the climate effects affecting the release of major greenhouse gases. .

To achieve the above object, the present invention can adopt the following technical solutions:

A mesocosmic culture device for simulating the effects of rainfall on plankton and greenhouse gases, comprising:

A base culture tank, which is a tank body with a closed bottom and an open upper end, and the side wall of the tank body is provided with a circulating pump, which is used to promote the circulation exchange of the water body in the base culture tank;

The top cover is set on the base cultivation tank, and the top cover is provided with a light-emitting device and a sprayer, the light-emitting device is used for simulating the irradiation of sunlight, and the sprayer is used for simulating rainfall.

The above-mentioned mesocosm cultivation device further includes a water quality analyzer, the water quality analyzer is arranged on the side wall of the cultivation tank base through the water quality analyzer hanger, and the water quality analyzer is provided with a probe, so the The probe is in contact with the water body in the base culture tank, and is used to collect various parameters of the water body in the experimental process.

The above-mentioned mesocosm culture device further includes a micro-electrode, the micro-electrode is movably arranged on the side wall of the base of the culture cylinder, and is used to detect the greenhouse gas in the base of the culture cylinder in the experimental process of the parameters.

In the above-mentioned mesocosm culture device, further, the bottom of the top cover is provided with an engaging groove that cooperates with the top of the base culture cylinder, and both the top cover and the base culture cylinder are made of resin material The circulating pump is provided with a water inlet and a water outlet, and the water body in the pedestal culture tank enters water from the water inlet side and discharges water from the water outlet side to realize the circulation exchange of the water body.

In the above-mentioned mesocosm cultivation device, further, the base cultivation tank is provided with a water valve that can adjust the flow rate of the water flow, which can be used for water sampling by opening the water valve.

The above-mentioned mesocosm culture device, further, the side wall of the base culture tank is provided with a plurality of connection ports, and the connection ports are used for connecting with an adjustable syringe to sample the Greenhouse gases in the base of the culture tank.

In the above-mentioned mesocosm culture device, further, a micro-electrode base is provided between the micro-electrode and the side wall of the base of the culture cylinder, and the micro-electrode base is provided with a horizontally extending lateral track and a vertical extending the longitudinal track, the micro-electrodes are slidably connected to the transverse track and the longitudinal track through a slider.

The above-mentioned mesocosm culture device, further, the sprayers are arranged at the four top corners of the top cover, and each of the sprayers is connected to a water source through a silicone hose to supplement the water flow consumed during the experiment. The sprayer is also provided with a spray regulator for setting the speed of the spray to simulate rainfall intensity.

In the above-mentioned mesocosm cultivation device, further, the illuminator is arranged at the center of the top cover, and the illuminator is provided with a dimming knob, and the dimming knob is used to set the luminous intensity to simulate sunlight intensity and UV intensity.

The above-mentioned mesocosm culture device further includes a voltage regulator, the power cord assembly of the sprayer and the cable of the light-emitting device are respectively connected to the voltage regulator, and the voltage regulator is connected through a socket connect power supply.

Compared with the prior art, the present invention has the beneficial effects that on the basis of observing the community structure and ecological function of primary producers of water bodies by rainfall, the present invention simultaneously observes bacterial activities mainly related to greenhouse gases, and determines the rainfall process. How will it affect the main greenhouse gas emission process, and at the same time, while determining the ecological effect of rainfall on the water body, the impact of rainfall on the main greenhouse gas production process can be observed. provide important scientific support for the study of climate effects.

Description of drawings

In order to illustrate the technical solutions in the embodiments of the present invention more clearly, the accompanying drawings required in the embodiments will be briefly introduced below. Obviously, the drawings in the following description are only some embodiments of the present application, and for those of ordinary skill in the art, other drawings can also be obtained from these drawings without creative effort.

Figure 1 is a schematic diagram of a mesocosmic culture device for simulating the effects of rainfall on plankton and greenhouse gas emissions;

Fig. 2 is the schematic diagram of the base pedestal cultivation tank of the mesocosmic cultivation device for simulating the effect of rainfall on plankton and greenhouse gas emissions;

Figure 3 is a schematic diagram of the upper roof of a mesocosmic culture device simulating the effects of rainfall on plankton and greenhouse gas emissions;

Fig. 4 is the schematic diagram of the micro-electrode hanger on the bottom base culture cylinder wall;

Figure 5 is a schematic illustration of the multiparameter analyzer hanger on the bottom pedestal incubator wall.

Among them: 1. Base culture tank; 2. Top cover; 3. Voltage regulator; 5. Resin material; 6. First engaging groove; 7. Connection port; 8. Circulating pump; 9. Water outlet; 10 , water inlet; 11, plug; 12, water valve; 13, microelectrode; 14, water quality analyzer; 15, sprayer; 16, illuminator; 17, second snap groove; 18, silicone hose; 20, wire cable; 21, power cord assembly; 22, socket; 23, horizontal track and vertical track; 24, microelectrode hanger; 25, microelectrode base; 26, analyzer base; 27, water quality meter hanger.

Detailed ways

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present application, not all of the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present application.

Example:

It should be noted that the terms "first", "second" and the like in the description and claims of the present invention and the above drawings are used to distinguish similar objects, and are not necessarily used to describe a specific sequence or sequence. It is to be understood that the data so used may be interchanged under appropriate circumstances such that the embodiments of the invention described herein can be practiced in sequences other than those illustrated or described herein. In addition, the terms "comprising" and "having" and any variations thereof in the embodiments of the present invention are intended to cover non-exclusive inclusion, for example, a process, method, system, product or device comprising a series of steps or units is not necessarily limited to Those steps or elements that are expressly listed may instead include other steps or elements that are not expressly listed or are inherent to the process, method, product or apparatus.

It is to be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "top", "bottom", "front", "rear", "left", "Right", "Vertical", "Horizontal", "Top", "Bottom", "Inner", "Outer", "Clockwise", "Counterclockwise", "Axial", "Radial", " The orientation or positional relationship indicated such as "circumferential direction" is based on the orientation or positional relationship shown in the accompanying drawings, and is only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the indicated device or element must have a specific orientation, It is constructed and operated in a particular orientation and is therefore not to be construed as a limitation of the present invention.

In the description of the present invention, "plurality" means at least two, such as two, three, etc., unless otherwise expressly and specifically defined. In addition, unless otherwise expressly specified and limited, the terms "installed", "connected" and "connected" should be understood in a broad sense, for example, it may be a fixed connection, a detachable connection, or an integral connection; it may be a mechanical connection. The connection can also be an electrical connection; it can be a direct connection, an indirect connection through an intermediate medium, or an internal connection between two components. For those of ordinary skill in the art, the specific meanings of the above terms in the present invention can be understood in specific situations.

In the present invention, unless otherwise expressly specified and limited, a first feature "on" or "under" a second feature may be in direct contact between the first and second features, or the first and second features indirectly through an intermediary touch. Also, the first feature being "above", "over" and "above" the second feature may mean that the first feature is directly above or obliquely above the second feature, or simply means that the first feature is level higher than the second feature. The first feature being "below", "below" and "below" the second feature may mean that the first feature is directly below or obliquely below the second feature, or simply means that the first feature has a lower level than the second feature.

Referring to Figures 1 to 5, Figure 1 is a schematic diagram of a mesocosm cultivation device for simulating the effects of rainfall on plankton and greenhouse gas emissions; Figure 2 is a base pedestal for a mesocosm cultivation device for simulating the effects of rainfall on plankton and greenhouse gas emissions Schematic diagram of a culture tank; Figure 3 is a schematic diagram of the upper top cover of a mesocosm culture device simulating the effect of rainfall on plankton and greenhouse gas emissions; Figure 4 is a schematic diagram of the microelectrode hanger on the bottom base culture tank wall; Figure 5 Schematic of the multiparameter analyzer hanger on the bottom pedestal tank wall.

The purpose of the present invention is to provide a device that can simulate the rainfall process in the field or in the laboratory, which can satisfy the ecological effect of observing the changes of plankton after the rainwater settles into the water body, and at the same time, through the greenhouse gas and production in the closed culture chamber The activity of microorganisms related to greenhouse gases in order to study the climate effects caused by rainfall.

A mesocosmic cultivation device for simulating the effects of rainfall on plankton and greenhouse gases, comprising: a base cultivation tank 1 and a top cover 2, wherein the base cultivation tank 1 is a tank body with a closed upper end opening at the bottom, and the The side wall of the tank body is provided with a circulating pump 8, and the circulating pump 8 is used to promote the circulation exchange of the water body in the base culture cylinder 1; the top cover 2 is covered on the base culture cylinder 1, so the The top cover 2 is provided with a light-emitting device 16 and a sprayer 15, the light-emitting device 16 is used to simulate the irradiation of sunlight, and the sprayer 15 is used to simulate rainfall. In this embodiment, the culturing device of the present invention is mainly composed of two parts: the lower base culturing cylinder 1 and the upper top cover 2, which can be connected by grooves (ie, engaging grooves); the base culturing cylinder 1 is composed of The bottom of the resin-made closed water tank, the bottom of the base culture tank 1 can be added with land rivers and lakes or ocean water, and the water body can be automatically circulated by the stepless speed-regulating circulating pump at the bottom, which can simulate the exchange rate of the water body. The upper center of the top cover 2 is equipped with a light-emitting device 16 that simulates sunlight and can adjust the light intensity. Around the light-emitting device 16, there are four sprayers 15 that simulate rainfall. The sprayers 15 are connected to an external voltage regulator 3, and the voltage regulator 3 can Adjust the spray intensity. The sprayer 15 is connected with an external silicone tube, which can directly pump the collected rainwater, and spray rainwater in the culture tank to simulate the rainfall process.

The above-mentioned medium universe cultivation device further includes a water quality analyzer 14, and the water quality analyzer 14 is arranged on the side wall of the cultivating tank base through the water quality analyzer hanger 27, and the water quality analyzer hanger 27 is provided with There is an analyzer base 26, the water quality analyzer 14 is provided with a probe, and the probe is in contact with the water body in the base culture tank 1, and is used to collect various parameters of the water body in the experimental process. In this embodiment, the multi-parameter water quality analyzer hanger is placed below the culturing liquid surface. When the multi-parameter water quality analyzer 14 is hung on it, the probe of the analyzer can be completely contacted with the liquid surface. Real-time monitoring of parameters such as temperature, salinity, chlorophyll, dissolved oxygen, pH and conductivity in water bodies.

The above-mentioned Mesocosm culture device further includes a micro-electrode 13, which is movably arranged on the side wall of the culture cylinder base, and is used to detect the micro-electrodes in the culture cylinder base in the experimental process. parameters of greenhouse gases.

In the above-mentioned mesocosm culture device, further, the bottom of the top cover 2 is provided with an engaging groove that is matched with the top of the base culture cylinder 1 (that is, the upper top cover is connected to the base through the second engaging groove 17 ). The top cover 2 and the base cultivating cylinder 1 are both made of resin material 5, and the circulating pump 8 is provided with a water inlet 10 and a water outlet 9. The water body in the base culturing tank 1 enters the water from the water inlet 10 side and exits the water outlet 9 side, so as to realize the circulation exchange of the water body. In this embodiment, one side of the water tank at the base of the culture tank is connected to a stepless speed regulation circulating pump 8, and the water body in the culture tank is sucked into water through one side, and the water is sprayed out from the other side, so that the culture tank is The realization of automatic circulation of the water body in the tank simulates the water body exchange process in the field environment.

In the above-mentioned mesocosm culture device, further, the base culture tank 1 is provided with a water valve 12 that can adjust the flow rate of the water flow, and the water valve 12 can be used for water sampling by opening the water valve 12 . In this embodiment, an adjustable opening (ie, the water valve 12 ) is provided at the lower position of the water body culturing area, which facilitates the collection of water samples without the exchange of air inside the culturing tank.

The above-mentioned mesocosm culture device, further, the side wall of the base culture cylinder 1 is provided with a plurality of connection ports 7, and the connection ports 7 are used to be connected with an adjustable syringe to pass the adjustable syringe. Greenhouse gases are sampled in the base of the incubator. In this embodiment, the connection port 7 on the upper part of the base of the culturing tank is an opening on the 3 side of the position above the liquid level of the culturing water in the culturing tank, which can be connected with a syringe and is usually closed. Connect a syringe to the adjustable syringe connection port 7 to collect gas.

In the above-mentioned mesocosm culture device, further, a micro-electrode base 25 is provided between the micro-electrode 13 and the side wall of the culture cylinder base, and the micro-electrode base 25 is provided with a horizontally extending lateral track and vertically extending longitudinal rails, the micro-electrodes 13 are slidably connected to the transverse rails and the longitudinal rails 23 through sliders. In this embodiment, the micro-electrode hanger 24 arranged on the remaining side of the upper part of the base of the culture cylinder is a 2-axis base that can be freely adjusted in the up-down and left-right directions. The electrode 13 slots are tightly connected. It should be noted that the micro-electrode hanger 24 is a base that can move infinitely in two axes. The micro-electrode 13 can be moved up and down, left and right through the inlet of the hanger, so that the micro-electrode 13 is located at a suitable position on the culture liquid surface.

The above-mentioned mesocosm culture device, further, the sprayers 15 are arranged near the four top corners of the top cover 2 and each of the sprayers 15 is connected to a water source through a silicone hose 18 to supplement the consumption during the experiment. The sprayer 15 is also provided with a spray regulator, which is used to set the speed of the spray to simulate the rainfall intensity. In this embodiment, the sprayer 15 of the upper top cover 2 is an electric suction-type spraying device. The speed is adjusted by the regulator, and the collected rainwater is sucked into a spraying state to spray the rainwater stably on the water surface of the bottom culture tank. , and the spraying speed can be adjusted to simulate rainfall intensity.

In the above-mentioned mesocosm culture device, further, the illuminator 16 is arranged at the center of the top cover 2, and the illuminator 16 is provided with a dimming knob, and the dimming knob is used to set the luminous intensity to Simulates the intensity of sunlight and UV intensity. In this embodiment, the sunlight simulation illuminator 16 has a stepless dimming knob on the upper part, which can freely adjust the intensity of the simulated sunlight and the intensity of ultraviolet rays, and is connected with the mobile phone APP; the upper part of the top cover 2 is provided with four external connection The rainwater pipeline simulates the rain sprayer 15, and the sprayer 15 can set the spraying speed through the middle regulator to simulate the rainfall intensity.

The above-mentioned mesocosm culture device further includes a voltage regulator 3, and the power cord assembly 21 of the sprayer 15 and the cable 20 of the light-emitting device 16 are respectively connected to the voltage regulator 3, so The voltage regulator 3 is connected to the power supply through the socket 22 .

The specific operation case of this device is as follows:

Fill the water body to be cultivated into the pedestal culture tank, mount the microelectrode for measuring greenhouse gases on the microelectrode hanger, mount the multi-parameter water quality meter on the water quality meter hanger, and set the microelectrode and the water quality meter. After it is in place and turned on, connect the upper top cover to the lower base culture tank, and seal the interface with silicone grease to prevent the exchange with external air. The silicone hose with the top cap connected to the sprayer is connected to the collected rainwater bottle.

After the device is fully connected, after connecting the above devices, turn on the simulated fluorescent lamp (ie, the illuminator) and the infinitely adjustable circulating pump connected to the culture tank, so that the water body can circulate according to the set speed. After the meter data is stable, turn on the sprayer and adjust the sprayer speed according to the required rainfall intensity. So far, the device has realized the process of simulating the impact of rainfall on plankton and bacteria, and monitored the emissions of major greenhouse gases during this process.

In the description of this specification, description with reference to the terms "one embodiment," "some embodiments," "example," "specific example," or "some examples", etc., mean specific features described in connection with the embodiment or example , structure, material or feature is included in at least one embodiment or example of the present invention. In this specification, schematic representations of the above terms are not necessarily directed to the same embodiment or example. Furthermore, the particular features, structures, materials or characteristics described may be combined in any suitable manner in any one or more embodiments or examples. Furthermore, those skilled in the art may combine and combine the different embodiments or examples described in this specification, as well as the features of the different embodiments or examples, without conflicting each other.

The above-mentioned embodiments are only to illustrate the technical concept and characteristics of the present invention, and the purpose thereof is to enable those of ordinary skill in the art to understand the content of the present invention and implement them accordingly, and not to limit the protection scope of the present invention. All equivalent changes or modifications made according to the essence of the present invention shall be included within the protection scope of the present invention.

Claims (10)

1. a mesocosmic culturing device for simulating the impact of rainfall on plankton and greenhouse gases, is characterized in that, comprising: A base culture tank, which is a tank body with a closed bottom and an open upper end, and the side wall of the tank body is provided with a circulating pump, which is used to promote the circulation exchange of the water body in the base culture tank; The top cover is set on the base cultivation tank, and the top cover is provided with a light-emitting device and a sprayer, the light-emitting device is used for simulating the irradiation of sunlight, and the sprayer is used for simulating rainfall. 2. The mesocosm cultivation device according to claim 1, further comprising a water quality analyzer, and the water quality analyzer is arranged on the side wall of the cultivation tank base through a water quality analyzer hanger, and the water quality analyzer The instrument is provided with a probe, and the probe is in contact with the water body in the base culture tank, and is used to collect various parameters of the water body in the experimental process. 3. The mesocosm culturing device according to claim 1, characterized in that, further comprising a micro-electrode, which is movably arranged on the side wall of the culturing cylinder base, and is used to detect the culturing of the experimental process. Parameters of greenhouse gases in the base of the cylinder. 4 . The mesocosm culture device according to claim 1 , wherein the bottom of the top cover is provided with an engaging groove matched with the top of the base culture tank, and the top cover and the base are provided with an engaging groove. 5 . The cultivation tanks are all made of resin material. The circulating pump is provided with a water inlet and a water outlet. The water body in the base cultivation tank enters the water from the water inlet side and flows out from the water outlet side to realize the water body. cyclic exchange. 5 . The mesocosm cultivation device according to claim 1 , wherein the base cultivation tank is provided with a water valve that can adjust the flow rate of water flow, which can be used for water sampling by opening the water valve. 6 . 6 . The mesocosm culture device according to claim 1 , wherein a plurality of connection ports are opened on the side wall of the base culture tank, and the connection ports are used for connecting with an adjustable syringe to pass the adjustable syringe. 7 . A regulated syringe samples greenhouse gases within the base of the incubator. 7. The mesocosm culture device according to claim 3, wherein a microelectrode base is provided between the microelectrode and the side wall of the base of the culture cylinder, and the microelectrode base is provided with a horizontal extension A transverse track and a vertically extending longitudinal track, the micro-electrodes are slidably connected to the transverse track and the longitudinal track through a slider. 8. The mesocosm cultivation device according to claim 1, wherein the sprayer is arranged at four corners close to the top cover and each of the sprayers is connected to a water source through a silicone hose to supplement the experimental process The sprayer is also provided with a spray regulator for setting the speed of the spray to simulate rainfall intensity. 9 . The mesocosm cultivation device according to claim 1 , wherein the light-emitting device is arranged at the center of the top cover, and the light-emitting device is provided with a dimming knob, and the dimming knob is used for setting Luminous intensity to simulate the intensity of sunlight and UV intensity. 10. The mesocosm culture device according to any one of claims 8 or 9, further comprising a voltage regulator, and the power cord assembly of the sprayer and the cable of the light-emitting device are respectively connected to the voltage A regulator, the voltage regulator is connected to the power supply through the socket.

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