CN105087355A - Photo-bioreactor system for breeding algae - Google Patents
Photo-bioreactor system for breeding algae Download PDFInfo
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Abstract
The invention discloses a photo-bioreactor system for breeding algae, wherein the system comprises a reactor, wherein algae and a culture solution for breeding the algae are accommodated inside the reactor in a sealed mode; a gas supply system which is communicated with the reactor and is used for supplying gas to the reactor for photo-bioreaction of the algae; a gas quality measuring device which is connected to the gas supply system and is used for measuring the gas supply quantity of the gas supply system; a culture solution supplementing device which is communicated with the reactor and is used for supplementing the algae culture solution to the reactor; an alkaline liquid supplementing device which is communicated with the reactor and is used for supplementing alkaline liquid to the reactor; a measuring instrument which is connected to the reactor and is used for measuring various parameter values inside the reactor in real time; and a control device which is used for controlling the gas supply system, the gas quality measuring device, the culture solution supplementing device and the alkaline liquid supplementing device to work according to various parameter values which are monitored by the measuring instrument in real time, so that various parameter values inside the reactor are controlled to achieve optimal culture condition values of the algae. The system can be used for improving the quality and the efficiency of algae culture.
Description
Technical field
Algae culture technical field of the present invention, particularly, relates to a kind of photosynthesis physiological target system for cultivating algae.
Background technology
Photosynthesis physiological target realizes the industrialized most important link of photosynthetic organism technical products.It is the bridge connecting molecular biology and cytobiology upstream technology and photosynthetic organism technology industrialization, is also bio-science, biological chemistry, the multi-disciplinary cross discipline product such as computer science and engineering.The fundamental difference of photosynthesis physiological target and existing various bio-reactor is that its demand fulfillment photosynthetic organism is to light quality, light intensity and photoperiodic requirement.The Application Areas of photosynthesis physiological target is extensive, is mainly blue-green algae, micro-algae, vegetable cell and the cultivation of tissue, the cell breeding of large algae and nursery etc.All need to use different photosynthesis physiological target in research, development and production, after reaction volume expands, the supply of light is often restricted, and especially when cell density reaches higher, the light-receiving area volume ratio improving reactor becomes one of important parameter of bioreactor design.Consider to require to mix in reactor from Object of Development, shearing force is little, and gas is for specified rate with even, and good seal performance, inoculates, adds (row) material and long time running not easily microbiological contamination etc.Although consider the above-mentioned technical problem that should be noted that, but the quality of existing bioreactor culture algae and efficiency are still very low.
Summary of the invention
Embodiments provide a kind of photosynthesis physiological target system for cultivating algae, in order to improve quality and the efficiency of algae culture, this system comprises:
Reactor, its seal inside is contained with algae and the nutrient solution for cultivating algae;
Airing system, is communicated with reactor, for providing the gas needed for the reaction of algae photosynthetic organism for reactor;
Gaseous mass measuring apparatus, is connected with airing system, for measuring the air demand of airing system;
Nutrient solution supplementary device, is communicated with reactor, for supplementing Algae culture solution for reactor;
Alkali lye supplementary device, is communicated with reactor, for supplementing alkali lye for reactor;
Metrical instrument, is connected with reactor, for measuring each parameter value in reactor in real time;
Control device, be connected with airing system, gaseous mass measuring apparatus, nutrient solution supplementary device, alkali lye supplementary device and metrical instrument, for each parameter value according to metrical instrument Real-Time Monitoring, control the work of airing system, gaseous mass measuring apparatus, nutrient solution supplementary device and alkali lye supplementary device.
In one embodiment, metrical instrument comprises: dissolved oxygen meter, for measuring the dissolution degree of carbonic acid gas in reactor;
Control device is specifically for the measuring result according to dissolved oxygen meter, and controlling airing system is supplement carbon dioxide in reactor.
In one embodiment, metrical instrument, comprising: pH instrument, for measuring the pH value in reactor;
Control device specifically for when pH instrument monitoring to the pH value in reactor lower than preset value time, controlling alkali lye supplementary device is that reactor supplements alkali lye.
In one embodiment, metrical instrument, comprising: light turbidometer, for the density of algae in monitoring reactor;
Control device is specifically for according to the turbidimetric monitoring result of light, and controlling nutrient solution supplementary device is that reactor supplements Algae culture solution, or controls algae culture and terminate.
In one embodiment, the above-mentioned photosynthesis physiological target system for cultivating algae also comprises: cultivate liquid pump, its one end is communicated with reactor, and the other end is communicated with nutrient solution supplementary device;
Control device, specifically for the measuring result according to metrical instrument, controls the speed of cultivating liquid pump, for reactor supplements the nutrient solution of corresponding discharge.
In one embodiment, the above-mentioned photosynthesis physiological target system for cultivating algae also comprises: lye pump, and its one end is communicated with reactor, and the other end is communicated with alkali lye supplementary device;
Control device, specifically for the measuring result according to metrical instrument, controls the speed of lye pump, for reactor supplements the alkali lye of corresponding discharge.
In one embodiment, the above-mentioned photosynthesis physiological target system for cultivating algae also comprises: gas and vapor permeation filtration unit, its one end is communicated with reactor, and the other end is communicated with airing system.
In one embodiment, airing system comprises: nitrogen air feeder;
Gaseous mass measuring apparatus comprises: nitrogen gas mass flowmeter, for measuring the air demand of nitrogen air feeder;
Control device, specifically for the measurement according to nitrogen gas mass flowmeter, controls nitrogen air feeder, for reactor supplements the nitrogen of corresponding discharge.
In one embodiment, airing system comprises: oxygen air feeder;
Gaseous mass measuring apparatus comprises: oxygen gas mass flowmeter, for measuring the air demand of oxygen air feeder;
Control device, specifically for the measurement according to oxygen gas mass flowmeter, controls oxygen air feeder, for reactor supplements the oxygen of corresponding discharge.
In one embodiment, airing system comprises: carbonic acid gas air feeder;
Gaseous mass measuring apparatus comprises: carbonic acid gas mass flowmeter, for measuring the air demand of carbonic acid gas air feeder;
Control device, specifically for the measurement according to carbonic acid gas mass flowmeter, controls carbonic acid gas air feeder, for reactor supplements the carbon dioxide of corresponding discharge.
Compared with the existing bio-reactor for cultivating algae, the photosynthesis physiological target system for cultivating algae that the invention process provides, the each parameter value in reactor is measured in real time by metrical instrument, control device is according to each parameter value of metrical instrument Real-Time Monitoring, control gaseous mass measuring apparatus, nutrient solution supplementary device, the work of alkali lye supplementary device, make each parameter value in reactor reach the optimal culture condition numerical value of algae fast, thus improve quality and the efficiency of algae culture.
Accompanying drawing explanation
Accompanying drawing described herein is used to provide a further understanding of the present invention, forms a application's part, does not form limitation of the invention.In the accompanying drawings:
Fig. 1 is for cultivating the structural representation of the photosynthesis physiological target system of algae in the embodiment of the present invention;
Fig. 2 is the result schematic diagram of control device control pH instrument, nutrient solution supplementary device, alkali lye supplementary device, gas mass flow device in the embodiment of the present invention;
Fig. 3 is that in the embodiment of the present invention, light turbidometer is measured algae density in real time, measured CO2 rate of utilization and bottoms stream and add total amount schematic diagram;
Fig. 4 is the result schematic diagram that in the embodiment of the present invention, control device controls algal grown;
Fig. 5 is optical throughput and CO2 rate of utilization result schematic diagram in the embodiment of the present invention;
Fig. 6 is the result schematic diagram of control survey pH value, dissolved oxygen and oxygen consumption rate in the embodiment of the present invention;
Fig. 7 be in one embodiment of the invention for cultivate the photosynthesis physiological target system of algae control device in the structural representation of AT-MIO-16E-10DAQ plate.
Embodiment
For making the object, technical solutions and advantages of the present invention clearly understand, below in conjunction with embodiment and accompanying drawing, the present invention is described in further details.At this, exemplary embodiment of the present invention and illustrating for explaining the present invention, but not as a limitation of the invention.
Fig. 1 is that as shown in Figure 1, this system comprises for cultivating the structural representation of the photosynthesis physiological target system of algae in the embodiment of the present invention:
Reactor 10, its seal inside is contained with algae and the nutrient solution for cultivating algae;
Airing system 20, is communicated with reactor 10, for providing the gas needed for the reaction of algae photosynthetic organism for reactor 10;
Gaseous mass measuring apparatus 30, is connected with airing system 20, for measuring the air demand of airing system 20;
Nutrient solution supplementary device 40, is communicated with reactor 10, for supplementing Algae culture solution for reactor 10;
Alkali lye supplementary device 50, is communicated with reactor 10, for supplementing alkali lye for reactor 10;
Metrical instrument 60, is connected with reactor 10, for measuring each parameter value in reactor 10 in real time;
Control device 70, be connected with airing system 20, gaseous mass measuring apparatus 30, nutrient solution supplementary device 40, alkali lye supplementary device 50, metrical instrument 60, for the Real-Time Monitoring according to metrical instrument 60, control airing system 20, gaseous mass measuring apparatus 30, nutrient solution supplementary device 40 and alkali lye supplementary device 50 to work, make each parameter value in reactor 10 reach the optimal culture condition numerical value of algae.
The photosynthesis physiological target system for cultivating algae that the embodiment of the present invention provides, during work, the each parameter value in reactor 10 is measured in real time by metrical instrument 60, control device 70 is according to each parameter value of the Real-Time Monitoring of metrical instrument 60, control gaseous mass measuring apparatus 30, nutrient solution supplementary device 40 and alkali lye supplementary device 50 to work, make each parameter value in reactor 10 reach the optimal culture condition numerical value of algae fast, thus improve quality and the efficiency of algae culture.
In one embodiment, metrical instrument 60 comprises: dissolved oxygen meter 61, is connected with reactor 10, for measuring the dissolution degree of carbonic acid gas in reactor 10;
Control device 70 is specifically for the measuring result according to dissolved oxygen meter 61, and controlling airing system 20 is supplement carbon dioxide in reactor 10.
During concrete enforcement, when dissolved oxygen meter 61 measures the carbonic acid gas entered into by airing system 20 in reactor 10, there is part not to be dissolved in nutrient solution, so, just control the carbon dioxide that airing system 20 be supplementary respective amount in reactor 10.Such design, improves precision and the efficiency of algae culture.
In one embodiment, metrical instrument 60, comprising: pH instrument 62, is connected with reactor 10, for measuring the pH value in reactor 10;
Control device 70 is specifically for monitoring pH value in reactor 10 lower than preset value during when pH instrument 62, controls alkali lye supplementary device 50 and supplementing alkali lye for reactor 10.
During concrete enforcement, when pH instrument 62 monitors pH value in reactor 10 lower than preset value, control device 70 is just according to the pH value that pH instrument 62 detects, control alkali lye supplementary device 50 and supplement alkali lye for reactor 10, to regulate the potential of hydrogen in reactor 10, reach acid base equilibrium, make the optimal culture condition reaching algae in reactor fast, such design improves quality and the efficiency of algae culture.
In one embodiment, metrical instrument 60, comprising: light turbidometer 63, is connected with reactor 10, for the density of algae in monitoring reactor 10;
Control device 70, specifically for the monitoring result according to light turbidometer 63, controls nutrient solution supplementary device 40 and supplements Algae culture solution for reactor 10, or control algae culture terminates.
During concrete enforcement, when the monitoring result according to light turbidometer 63, if find, the density of algae in reactor 10 does not reach the degree of algae maturation, and so control device 70 controls nutrient solution supplementary device 40 and supplements nutrient solution for reactor 10; If find, the density of algae in reactor 10 has reached the degree of algae maturation, and so just control this system finishing and run, algae culture terminates, and such design improves quality and the efficiency of algae culture.
Certainly, control device 70 also according to artificial to the detected result in reactor 10, can control nutrient solution supplementary device 40 and supplements Algae culture solution for reactor 10.Such as: staff is the content of glucose in detection reaction device 10 auxiliarily, when detected result display, in reactor 10, the content of glucose is very deficient, and so staff is by control device 70, controls nutrient solution supplementary device 40 and supplements Algae culture solution for reactor 10.
Certain above-mentioned metrical instrument 60, except comprising dissolved oxygen meter 61, pH instrument 62 and light turbidometer 63, can also comprise other metrical instruments, such as, for measuring temperature sensor of temperature in reactor 10 etc.
In one embodiment, said system also comprises: cultivate liquid pump 80, its one end is communicated with reactor 10, and the other end is communicated with nutrient solution supplementary device 40;
Control device 70, specifically for the measuring result according to metrical instrument 60, controls the speed of cultivating liquid pump 80, for reactor 10 supplements the nutrient solution of corresponding discharge.
In one embodiment, said system also comprises: lye pump 90, and its one end is communicated with reactor 10, and the other end is communicated with alkali lye supplementary device 50;
Control device 70, specifically for the measuring result according to metrical instrument 60, controls the speed of lye pump 90, for reactor 10 supplements the alkali lye of corresponding discharge.
In one embodiment, said system also comprises: gas and vapor permeation filtration unit 100, and its one end is communicated with reactor 10, and the other end is communicated with airing system 20.Arranging of gas and vapor permeation filtration unit 100 can ensure that the gas entered in reactor 10 by airing system 20 is fully mixed, and for algae culture provides favourable condition, meanwhile, can also ensure the purity of the gas entered in reactor 10.
In one embodiment, airing system 20 comprises: nitrogen air feeder 21, is communicated with reactor 10;
Gaseous mass measuring apparatus 30 comprises: nitrogen gas mass flowmeter 31, for measuring the air demand of nitrogen air feeder;
Control device 70, specifically for the measurement according to nitrogen gas mass flowmeter 31, controls nitrogen air feeder 21, for reactor 10 supplements the nitrogen of corresponding discharge.
In one embodiment, airing system 20 comprises: oxygen air feeder 22, is communicated with reactor 10;
Oxygen gas mass flowmeter 32, for measuring the air demand of oxygen air feeder 22;
Control device 70, specifically for the measurement according to oxygen gas mass flowmeter 32, controls oxygen air feeder 22, for reactor 10 supplements the oxygen of corresponding discharge.
In one embodiment, airing system 20 comprises: carbonic acid gas air feeder 23, is communicated with reactor 10;
Carbonic acid gas mass flowmeter 33, for measuring the air demand of carbonic acid gas air feeder 23;
Control device 70, specifically for the measurement according to carbonic acid gas mass flowmeter 33, controls carbonic acid gas air feeder 23, for reactor 10 supplements the carbon dioxide of corresponding discharge.
During concrete enforcement, by arranging nitrogen gas mass flowmeter 31, oxygen gas mass flowmeter 32 and carbonic acid gas mass flowmeter 33, the gas volume being supplied to reactor 10 is measured, can realize needing how many just supplementary how many accurate control object, improve precision and the efficiency of algae culture.
The photosynthesis physiological target system for cultivating algae that the embodiment of the present invention provides, the controllable type that can realize algae is meticulous, intensive manufacture.Temperature in culturing process, pH, dissolved oxygen, CO2 can be implemented in line traffic control.This reactor can make several gasometry mix, and also can be controlled by the FR dissolving oxygen and carbon dioxide of automatic control realization.Automatic detection and control (can expand and the control more multiple reactor) while that this system can being carried out 16 photosynthesis physiological target.Reactor volume is from 500mL to 1000L.This photosynthesis physiological target system can indoors be used alone open air, is convenient to grope culture process, experiment condition, the polyfactorial affecting laws of investigation etc.
Fig. 2 is control device 70 control pH instrument 62, nutrient solution supplementary device 40, alkali lye supplementary device 50, nitrogen gas mass flowmeter 31, oxygen gas mass flowmeter 32 and carbonic acid gas mass flowmeter 33 result schematic diagram in the embodiment of the present invention; As shown in Figure 2, in Fig. 2, X-coordinate is the time of algae culture, ordinate zou is the mass rate passing into gas, in Fig. 2, curve respectively illustrates nitrogen (N2), oxygen (O2), carbonic acid gas (CO2), carbon dioxide solubility degree (DO) and pH value are with the changing conditions of incubation time, as shown in Figure 2, by the control of control device 70, the above-mentioned each parameter value in reactor 10 is made to reach the optimal culture condition numerical value of algae.
Fig. 3 is that in the embodiment of the present invention, light turbidometer 63 is measured algae density in real time, measured CO2 rate of utilization and bottoms stream and add total amount schematic diagram; As shown in Figure 3, in Fig. 3, X-coordinate is the time of algae culture, left side ordinate zou is CO2 release rate (No. 3 line), bottoms stream adds total amount (Line 1), right side ordinate zou is algae density (OD value, No. 2 lines), be CO2 release rate respectively in Fig. 3, bottoms stream adds total amount, algae density (OD value) with the changing conditions profile of incubation time, as shown in Figure 3, by the control of control device 70, the above-mentioned each parameter value in reactor 10 is made to reach the optimal values of algae culture.
Fig. 4 is the result schematic diagram that in the embodiment of the present invention, control device 70 controls algal grown; As shown in Figure 4, in Fig. 4, X-coordinate is the time of algae culture, left side ordinate zou is cell concn, comprise total cell concn (square curve) and via cell concn (diamond curve), right side ordinate zou is metabolite concentration (glucose-black line triangle curve, sucrose-asterisk curve).Be cell concn respectively in Fig. 4, via cell concn, dextrose plus saccharose, with the changing conditions curve of incubation time, as shown in Figure 4, by the control of control device 70, makes algae culture in reactor 10 reach good level of control.
Fig. 5 is optical throughput and CO2 rate of utilization result schematic diagram in the embodiment of the present invention; As shown in Figure 5, in Fig. 5, X-coordinate is the time of algae culture, and ordinate zou is Active Power Filter-APF (No. 7 line), and it represents optical throughput and CO2 release rate (No. 8 lines).Optical throughput and the CO2 rate of utilization changing conditions curve with incubation time respectively in Fig. 5.
Fig. 6 is control survey pH value in the embodiment of the present invention, the result schematic diagram of dissolved oxygen and oxygen consumption rate; As shown in Figure 6, in Fig. 6, X-coordinate is the time of algae culture, and left side ordinate zou is dissolved oxygen rate (DO, No. 6 lines) and pH value (No. 5 lines), and right side ordinate zou is oxygen consumption rate (OUR, Oxygenuptakerate, No. 4 lines).Be pH value respectively in Fig. 6, dissolved oxygen and oxygen consumption rate, with the changing conditions curve of incubation time, as shown in Figure 6, by the control of control device 70, make algae culture in reactor 10 reach good level of control.
Visible shown in Fig. 2 to Fig. 6 thus, the photosynthesis physiological target system for cultivating algae that the embodiment of the present invention provides, can realize accurate Measurement & Control automatically to the various parameters of algal grown, show good application prospect.
During concrete enforcement, the embodiment of the present invention provide for cultivating in the photosynthesis physiological target system of algae, control device 70 can be computer, also can be PLC programmable logic controller and singlechip controller etc., or their combination, as long as with airing system 20, gaseous mass measuring apparatus 30, nutrient solution supplementary device 40, alkali lye supplementary device 50, metrical instrument 60 connects, according to the Real-Time Monitoring of metrical instrument 60, control airing system 20, gaseous mass measuring apparatus 30, nutrient solution supplementary device 40 and alkali lye supplementary device 50 work, the each parameter value in reactor 10 is made to reach the optimal culture condition numerical value of algae.
During concrete enforcement, the embodiment of the present invention provide for cultivate algae photosynthesis physiological target system in control device comprise computer time, mainframe computer operating system can be WINDOWXP system, and computer external apparatus interface plate can be:
1.AT-MIO-16E-10DAQboard:16AI/2AO/8DIO；
2.AT-AO-10DAQboard:10AO；
3.PCI-232/4Board(4RS232ports)。
Control device not only can realize the control of control, pH and DO etc. to gas flow, the control to foam and liquid level etc. can also be realized, particularly, Mass Control under meter provides accurate CO2, O2, N2 and air flow quantity metering, and the calculating of gas and vapor permeation ratio can be carried out by Mass Control under meter, there is provided accurate stream to add control to low speed peristaltic pump (such as: cultivate liquid pump and lye pump), this control device can be monitored and nearly 16 overlap independently photosynthetic reactor, and can expand further.
The setting that example illustrates control device (comprising: computer) and peripheral device communication port is counted below: mainframe computer is by the serial port of the PC of standard with the gentle smooth turbidity in nutrient solution sky, RS-232 communications protocol is used to realize the communication connection of nutrient solution balance (EK-20) (this nutrient solution balance can be a part for nutrient solution supplementary device) and light turbidometer (LA-300GT), the gentle smooth turbidometer in nutrient solution sky is provided with 25 pin RS-232C male joints, and the RS-232C of 9 pin female connectors is for communication port.
As follows with the connection description of nutrient solution balance:
As follows with the connection description of light turbidometer:
During concrete enforcement, multi-RS-232board, PCI-232/4Board interface board for computers also has multiple LPT, can for other bioreactor.Data gathering during concrete enforcement and control signal transmit mouth (being not limited to following) as described below:
During concrete enforcement, analog input/output can be carried out by two NationalInstruments interface boards, numeral I/O sends and can be: AT-MIO-16E-10DAQ and AT-AO-10DAQ, Fig. 7 are that the connector pinout of AT-MIO-16E-10DAQ plate distributes schematic diagram.
During concrete enforcement, pH value (0 ~ 1V), DO (0 ~ 100mV), light intensity meter (0 ~ 100mV), oxygen, the signal of nitrogen and carbon dioxide flow rate (0 ~ 5V), batch feed pump (0 ~ 5V) is transmitted by AT-MIO-16E10DAQ plate.The mass flow controller (0 ~ 5V) of oxygen, nitrogen and carbonic acid gas, batch feed pump (0 ~ 5V) analog output signal is sent by AT-AO-10 data acquisition board, and numeral exports and comprises 2 entrance and exits for cultivating liquid pump and lye pump data gathering.Peristaltic pump (such as: cultivate liquid pump and lye pump): Watson-Marlow101U/R pump is used for feed supplement stream and adds, flow velocity should carry out demarcation calibration in the operating condition, most important influence factor sucks and transfer pressure, the viscosity of temperature and fluid, this pump can be operated in double-communication mode.
During concrete enforcement, the graphical programming software LabVIEW of NationalInstruments company can be adopted to write sequence of control, wherein, data presentation and storage can comprise:
1, the consumption of real-time data acquisition and display pH value, dissolved oxygen, light turbidity (density) value, light intensity value, oxygen consumption rate (OUR), oxygen and/or CO2;
2, the critical processes numerical value that dialog interface, summary screen display are all: set(ting)value, currency, master mode, unit, output and cascade have display, more have each parameter classification is controlled, setting, calibration and cascade expansion screen;
3, uploading data in experiment runs;
4, graphical programming provides data visualization pictorialization;
5, data directly can derive Excel form and carry out aftertreatment;
6, experiment can carry out Long-distance Control by internet;
7, above-mentioned variables/parameters is recorded in the file on hard disk.
Forecast and control strategy can comprise:
1, oxygen/CO2 and glucose utilization is estimated in real time;
2, the nutrient solution feed supplement of feeding culture calculates;
3, Adaptive PID Control dissolves CO2 level.
LabVIEW is a high-level graphical programming language, allows self-defined application program with coding effectively.The operational mode pre-programmed that system can be cultivated for alga cells, the gas flow between switching and stirring velocity scope regulate automatically.As all LabVIEW application programs, it has two independently parts, front panel and line chart.Be hidden in the line graphical presentation after front panel to export along the term LabVIEW from being input to from the data of front panel upper figure representative and index, so-called " network migrations of lines ", this data flow model makes LabVIEW algorithm have the character of concurrent operation, by the front panel of LabVIEW, can display light bioreactor processes variable and controling parameters.
Collected data can show in a different manner, and as graphical window or histogram, data transformations is become visualization digital by them, such as: " process variable " is graphical window, for every 2 seconds display updates; " PID output " is about carrying out to gas flow the control signal that PID controls gas according to state variables; " pH value, dissolved oxygen, external diameter and glucose " window is used to show these system variables, every 10 minutes display updates; " oxygen depletion speed and photosynthetic quotient " window, is used for showing the rate of consumption of oxygen and the calculation result of algae culture photosynthetic quotient; Histogram " oxygen flow ", " nitrogen flow " and " CO2 flow ", for showing these mass-flow gas meter measure flow rate.The Kai Heguan that three buttons are used to refer to (nutrient solution inputs, and waste liquid exports and alkali lye) pump specifically can be set.
The embodiment of the present invention provide for cultivating in the photosynthesis physiological target system of algae, control device 70 can control 7.5 liters of indoor photosynthesis physiological target, 500 milliliters of indoor photosynthesis physiological target and/or 15-50 and rise outdoor photosynthesis physiological target.
Advantageous Effects of the present invention is: compared with the existing bio-reactor for cultivating algae, the photosynthesis physiological target system for cultivating algae that the invention process provides, the each parameter value in reactor is measured in real time by metrical instrument, control device is according to the Real-Time Monitoring of metrical instrument, control gaseous mass measuring apparatus, nutrient solution supplementary device, the work of alkali lye supplementary device, make each parameter value in reactor reach the optimal culture condition numerical value of algae fast, thus improve quality and the efficiency of algae culture.
Above-described specific embodiment; object of the present invention, technical scheme and beneficial effect are further described; be understood that; the foregoing is only specific embodiments of the invention; the protection domain be not intended to limit the present invention; within the spirit and principles in the present invention all, any amendment made, equivalent replacement, improvement etc., all should be included within protection scope of the present invention.
Claims (10)
1., for cultivating a photosynthesis physiological target system for algae, it is characterized in that, comprise:
Reactor, its seal inside is contained with algae and the nutrient solution for cultivating algae;
Airing system, is communicated with described reactor, for providing the gas needed for the reaction of algae photosynthetic organism for described reactor;
Gaseous mass measuring apparatus, is connected with described airing system, for measuring the air demand of described airing system;
Nutrient solution supplementary device, is communicated with described reactor, for supplementing Algae culture solution for described reactor;
Alkali lye supplementary device, is communicated with described reactor, for supplementing alkali lye for described reactor;
Metrical instrument, is connected with described reactor, for measuring each parameter value in described reactor in real time;
Control device, be connected with described airing system, gaseous mass measuring apparatus, nutrient solution supplementary device, alkali lye supplementary device and metrical instrument, for each parameter value according to described metrical instrument Real-Time Monitoring, control described airing system, gaseous mass measuring apparatus, nutrient solution supplementary device and the work of alkali lye supplementary device.
2. as claimed in claim 1 for cultivating the photosynthesis physiological target system of algae, it is characterized in that, described metrical instrument comprises: dissolved oxygen meter, for measuring the dissolution degree of carbonic acid gas in reactor;
Described control device is specifically for the measuring result according to described dissolved oxygen meter, and controlling described airing system is supplement carbon dioxide in described reactor.
3. as claimed in claim 1 for cultivating the photosynthesis physiological target system of algae, it is characterized in that, described metrical instrument, comprising: pH instrument, for measuring the pH value in reactor;
Described control device specifically for when pH instrument monitoring to the pH value in reactor lower than preset value time, controlling described alkali lye supplementary device is that described reactor supplements alkali lye.
4. as claimed in claim 1 for cultivating the photosynthesis physiological target system of algae, it is characterized in that, described metrical instrument, comprising: light turbidometer, for the density of algae in monitoring reactor;
Described control device is specifically for according to the turbidimetric monitoring result of light, and controlling described nutrient solution supplementary device is that described reactor supplements Algae culture solution, or controls algae culture and terminate.
5. as claimed in claim 1 for cultivating the photosynthesis physiological target system of algae, it is characterized in that, also comprise: cultivate liquid pump, its one end is communicated with described reactor, and the other end is communicated with described nutrient solution supplementary device;
Described control device, specifically for the measuring result according to metrical instrument, controls the speed of described cultivation liquid pump, for described reactor supplements the nutrient solution of corresponding discharge.
6. as claimed in claim 1 for cultivating the photosynthesis physiological target system of algae, it is characterized in that, also comprise: lye pump, its one end is communicated with described reactor, and the other end is communicated with described alkali lye supplementary device;
Described control device, specifically for the measuring result according to metrical instrument, controls the speed of described lye pump, for described reactor supplements the alkali lye of corresponding discharge.
7. as claimed in claim 1 for cultivating the photosynthesis physiological target system of algae, it is characterized in that, also comprise: gas and vapor permeation filtration unit, its one end is communicated with described reactor, and the other end is communicated with described airing system.
8. as claimed in claim 1 for cultivating the photosynthesis physiological target system of algae, it is characterized in that, described airing system comprises: nitrogen air feeder;
Described gaseous mass measuring apparatus comprises: nitrogen gas mass flowmeter, for measuring the air demand of nitrogen air feeder;
Described control device, specifically for the measurement according to nitrogen gas mass flowmeter, controls described nitrogen air feeder, for described reactor supplements the nitrogen of corresponding discharge.
9. as claimed in claim 1 for cultivating the photosynthesis physiological target system of algae, it is characterized in that, described airing system comprises: oxygen air feeder;
Described gaseous mass measuring apparatus comprises: oxygen gas mass flowmeter, for measuring the air demand of oxygen air feeder;
Described control device, specifically for the measurement according to oxygen gas mass flowmeter, controls described oxygen air feeder, for described reactor supplements the oxygen of corresponding discharge.
10. as claimed in claim 1 for cultivating the photosynthesis physiological target system of algae, it is characterized in that, described airing system comprises: carbonic acid gas air feeder;
Described gaseous mass measuring apparatus comprises: carbonic acid gas mass flowmeter, for measuring the air demand of carbonic acid gas air feeder;
Described control device, specifically for the measurement according to carbonic acid gas mass flowmeter, controls described carbonic acid gas air feeder, for described reactor supplements the carbon dioxide of corresponding discharge.
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