管道气升式磁处理光生物反应器微藻生产系统及监控方法 Microalgae photobioreactor system and method for monitoring airlift pipe Magnetization
本发明是管道气升式磁处理光生物反应器微藻生产系统及其监控方法，属生物工程技术领域，特别涉及微藻的生产设备与优化控制技术。 The present invention is a pipeline processing optical magnetic airlift bioreactor system and a monitoring method microalgae, belongs to the field of biological engineering, and in particular relates to optimization of microalgae production equipment control technology.
目前国内外的螺旋藻、小球藻、杜氏藻等微藻的养殖普遍采用开放式浅水道生产系统。 Spirulina is currently at home and abroad, Chlorella, Dunaliella and other microalgae breeding widespread use of open shallow water channel production systems. 尽管其操作比较简单，但其存在着如下不足：(1)由于受系统外环境的影响，难于保持微藻养殖的最佳条件；(2)由于是开放式生产系统，会很易受到灰尘、昆虫、杂菌的污染，故难于实行高质量的纯种养殖；(3)由于是浅水道生产系统，水分容易蒸发，造成其盐度增加，使微藻生长速度缓慢；(4)由于此种养殖系统的光路较长，光照面积与体积之比较低，致使光能和CO2的利用率不高，无法实现高细胞密度生物养殖；(5)占地面积大、藻液浓度低、微藻产量低，如螺旋藻的产量仅为0.5g/L(细胞干重)左右，因此生产成本高、收获费用高。 Despite its operation is relatively simple, but there are the following defects: (1) due to the influence by the environment outside of the system, it is difficult to maintain optimum conditions of microalgae culture; (2) production systems because it is open, it will be vulnerable to dust, insects, bacteria contamination, it is difficult to implement high-quality pure culture; (3) the production system because it is shallow channel, water easily evaporates and the salinity increases, microalgae slow growth; (4) due to this longer light path culture systems, the illumination area to volume is relatively low, resulting in energy and CO2 utilization is not high, can not achieve a high cell density culture organisms; large (5) covers an area of low concentration of algae, microalgae production low, such as Spirulina produced only 0.5g / L (dry cell weight) or so, and therefore high production costs and high harvesting costs.
本发明的目的是研究设计出一种新型高效的、可实现生产过程自动监测与控制、培养与收获一体化、可实现高细胞密度连续性生产的全封闭管道气升式循环磁处理光生物反应器微藻生产系统及其监控方法。 Study object of the present invention is to design a new and efficient, automatic monitoring and control of the production process, the integration of culture and harvest, can achieve high cell density continuous production of pipe closed magnetic treatment airlift photobioreactor is microalgae production system and monitoring method. 将流体力场、光场与磁场等物理场有机结合，达到强化微藻生长过程的目的，并采用在线检测与计算机监控等新技术实现生产过程的优化控制，从而克服和解决现有微藻生产系统所存在的难于保持微藻养殖最佳条件、占地面积大、微藻产量低、成本高且无法实现高细胞密度生物养殖等缺点和问题。 The physics fluid force field, magnetic field and light, and combination to achieve the purpose of strengthening the microalgae growth process, and the introduction of new technologies and computer line detection monitoring to optimize control of the production process, to overcome and to solve the conventional microalgae the existing system is difficult to maintain optimum conditions for microalgae cultivation, large footprint, low microalgae production, high cost and can not achieve a high cell density bio-farming and other shortcomings and problems.
本发明是通过下述结构技术方案和方法技术方案来实现的：管道气升式循环磁处理光生物反应器微藻生产系统的结构组成示意图如图1所示，它主要由管道气升式磁处理光生物反应器主体、加料装置、供气装置、收获装置及监控装置五部分构成，具体构成是：它由带式输送机1、回转过滤机2、电泵3、空气压缩机4、通气管道5、空气过滤器6、取液管道7、总气流控制阀8、空气控制阀9、气体流量计10、CO2钢瓶11、气体流量计12、气升室13、恒定磁场可调装置14、CO2控制阀15、热交换器16、除气室(贮液罐)17、挡板18、溢出管19、温度传感器20、pH电极21、温度仪22、排气管23、pH测定仪2 4、溶解氧测定仪25、计算机26、光照度计27、O2电极28、浊度仪29、加液管30、浊度传感器31、液体流量计32、光强度控制装置33、测光探头34、萤光灯35、气降室(受光管道)36、蠕动泵37、 The present invention is achieved by the following technical solutions and structural solutions METHOD: airlift pipe processing optical magnetic structure microalgae bioreactor system shown in Figure 1. The composition, which is mainly composed of magnetic airlift pipe processing photobioreactor body, charging means, supply means, and control means harvesting device five parts, a specific configuration of: it consists of a belt conveyor 1, the rotary filter 2, pump 3, the air compressor 4, the vent duct 5, the air filter unit 6, the liquid conduit 7, the total gas flow control valve 8, the air control valve 9, the gas flow meter 10, CO2 cylinder 11, gas flow meter 12, the air lift chamber 13, a constant magnetic field device 14 is adjustable, CO2 control valve 15, the heat exchanger 16, the degassing chamber (reservoir tank) 17, a shutter 18, an overflow pipe 19, a temperature sensor 20, pH electrode 21, temperature gauge 22, exhaust pipe 23, pH meter 24 , dissolved oxygen meter 25, computer 26, the light meter 27, O2 electrodes 28, 29 turbidimeter, adding the liquid pipe 30, the turbidity sensor 31, the liquid flow meter 32, the light intensity controller 33, probe 34 photometry, firefly light 35, gas descending chamber (light pipe) 36, a peristaltic pump 37, 液控制阀38、新鲜培养液贮存罐39共同相互连接构成，其相互连接关系为：将数支萤光灯35依次并列排列，再将气降室(受光管道)36围绕萤光灯35缠绕数圈，气降室36上端向上引出依次穿过恒定磁场可调装置14和热交换器16与除气室17相连接，除气室17中间安装挡板18，其正上方安装引出排气管23，其左右两侧安装引出溢出管19和加液管30，溢出管19装于除气室内略高于挡板18处，加溢管30安装于略高于溢出管19之处，在除气室17的左下部安装引出气升室13与通气管5相连接后再与气降室(受光管道)36相互连接构成整个光生物反应器的循环通路，在气升室13与气降室36的连接处安装引出取液管7，液体流量计32安装于恒定磁场可调装置14上下方与气降室36相连接，构成管道气升式磁处理光生物反应器主体；新鲜培养液贮存罐39通过加液控制阀38及管道与蠕动泵37相连接后 Fluid control valve 38, fresh medium storage tank 39 which are interconnected together, which is connected to the relationship between: the number of fluorescent lamp 35 are sequentially arranged in parallel branches, and then the gas descending chamber (light pipe) 36 is wound around a number of fluorescent lamps 35 ring, the upper end of the air chamber 36 drop passes through a constant magnetic field drawn upwardly adjustable means 14 and the heat exchanger 16 and the degassing chamber 17 is connected to the degassing chamber 17 intermediate the bezel 18, which is mounted directly above an exhaust pipe 23 leads , left and right sides leads overflow pipe 19 is mounted and the charging tube 30, the overflow pipe 19 to the degassing chamber mounted slightly above the shutter 18, plus overflow tube 30 is attached to the overflow pipe 19 is slightly higher than, the degassing the lower left portion of chamber 17 is mounted lead airlift chamber circulation passage after 13 connected to the breather pipe 536 interconnecting the gas descending chamber (light pipe) constitute the entire optical bioreactor, in an airlift chamber 13 and the gas down chamber 36 junction pipe 7 was taken mounting lead, a constant liquid flow meter 32 is attached to the adjustable magnetic field on the gas down below the chamber 14 means 36 is connected to the pipe constituting the magnetic treatment airlift photobioreactor body; fresh medium storage tank 38 with the peristaltic pump 37 and pipe 39 is connected to control valve Dosing 再通过管道与除气室17右上侧的加液管30相连接构成加料装置，CO2钢瓶11通过CO2控制阀15及管道与气体流量计12相连接，空气压缩机4通过空气过滤器6及空气控制阀9及管道与气体流量计10相连接；气体流量计10、12的引出管道并联连接后，再通过总气流控制阀8及管道与通气管道5相连接，这就构成了系统的供气装置；带式输送机1通过传送带与回转过滤机2相连接，再通过其贮液槽与溢出管19相连接构成系统的收获装置；温度传感器20、pH电极21、O2电极28、浊度传感器31依序置于除气室(贮液罐)17内的液面以下，再分别与温度仪22、pH测定仪24、溶解氧测定仪25、浊度仪29相连接，测光探头34置于气降室36中间外部朝向萤光灯35处并与光照度计27相连接，温度仪22、pH测定仪24、溶解氧测定仪25、光照度计27、浊度仪29均分别通过计算机的不同输入端口与计算机26相连接 Then connected to the charging device configured, 11 12 CO2 cylinder is connected via a flow meter 15 and the conduit and the gas control valve by adding CO2 liquid pipe 30 and plenum pipe 17 in addition to the upper right, the air compressor 4 through the air filter 6 and the air the control valve 9 and the duct 10 is connected with a gas flow meter; flowmeter after gas discharge conduit 10, 12 is connected in parallel, and then connected to the control valve 8 and the duct 5 with the total air flow the air duct, which constitutes a gas supply system apparatus; belt conveyor 12 is connected to the rotary filter by a conveyor belt, and then harvested by means of the system constituting the sump and which is connected to the overflow pipe 19; 28, turbidity sensor temperature sensor 20, pH electrode 21, O2 electrode 31 are sequentially placed in the degassing chamber (reservoir tank) 17 of the liquid level, and then the temperature device 22, pH measuring device 24, respectively, dissolved oxygen meter 25, 29 is connected to turbidity, the probe 34 is set photometry gas down to the intermediate chamber 36 toward the outside of the fluorescent lamp 35 and 27 is connected to the light meter, temperature gauge 22, pH meter 24, dissolved oxygen meter 25, the light meter 27, 29 are respectively turbidimeter different computers the input port 26 is connected to the computer 空气控制阀9、CO2控制阀15、光强度控制装置33、加液控制阀38通过计算机的不同输出端。 Air control valve 9, CO2 control valve 15, the light intensity control means 33, 38 controlled by a computer Dosing different output end valve. 与计算机26相连接共同构成监测与控制部分。 And computer 26 are connected together and configured to monitor control section.
本生产系统的计算机监控原理方框图如图2所示，其监控程序流程图如图3所示。 Computer monitoring block diagram of the principle of the production system shown in Figure 2, which monitors the program flow chart shown in Figure 3. 其监控方法如下：从设置于光生物反应器除气室17内的温度传感器20、pH电极21、O2电极28、浊度传感器31及设置于气降室36中的测光探头34中获取相应的各类参数的电信号，经A/D转换器40转换成数字信息通过串行接口输入计算机26以实现微藻参数的检测与处理。 Monitoring method which is as follows:, pH electrode 21, 28, the turbidity sensor 31 and the photometry sensor 34 disposed in the gas descending chamber 36 is acquired from the O2 electrode in addition to the temperature sensor 17 is disposed within the plenum to a respective photobioreactor 20 the various parameters of the electrical signal converted by the a / D converter 40 into digital information into the computer via the serial interface 26 to enable detection and processing parameters microalgae. 通过D/A转换器41将从计算机26输出的数字信号转化为模拟信号，再分别传送至空气控制阀9及CO2控制阀15来控制通气量的大小、CO2比例，从而控制藻液的pH值、循环速度、溶解氧浓度。 Output from the computer 26 via the D / A converter 41 into an analog signal into a digital signal, and then were transferred to a CO2 air control valve 9 and control valve 15 to control the magnitude of ventilation, CO2 ratio, thereby controlling the pH of the algae solution , circulation rate, dissolved oxygen concentration. 然后通过PIO转换器45输出高低电压构成的各种信息并分别传送至新鲜培养液加液控制阀38来控制加进新鲜培养液的量多少，通过光强度控制装置33及收获电泵3控制微藻所受光强度大小、生物量浓度、收获速度，从而调控微藻的生长，实现微藻生产过程的在线优化监测与控制。 Then various kinds of information through the low voltage output constituting the PIO converter 45 and are transferred to fresh culture medium was added to control the control valve 38 add much amount of fresh medium, harvesting device 33 and the micro-pump 3 is controlled by the light intensity control alginic suffered light intensity level, biomass concentration, harvesting speed, thereby regulating the growth of algae, microalgae production process for online monitoring and control optimization. 其中光强度的控制是通过控制工作(点亮)萤光灯的支数来实现，藻液温度的控制是根据不同的藻种来保持最佳生长温度范围。 Wherein the light intensity control is achieved by controlling the operation (lighting) of the fluorescent lamp count, temperature control algae solution is to maintain optimum growth temperature range depending on the algae species. 由于微藻生长过程的信息获取与处理并不要求十分迅速，所以其控制程序可用BASIC语言按图3所示的计算机监控流程图进行编制，计算机26还可以把优化监控资料输送到监控显示器43进行实时显示和输送到打印机44进行打印。 Since the growth of microalgae information acquisition and processing procedure does not require very rapidly, so that the control program can be compiled BASIC language computer monitoring by the flowchart shown in FIG. 3, the computer 26 may also monitor data to optimize the delivery to the monitor 43 for display real-time display and delivered to the printer 44 for printing.
本发明与现有技术相比有如下优点和有益效果：(1)由于本微藻养殖系统引入磁场进行磁处理，利用生物的磁效应，可以显著提高微藻的生长速度、增加产量，并能改善如氨基酸、蛋白质、多糖等营养组成的含量；(2)由于采用管道气升连续循环方式，能产生稳定均匀的搅拌效果，可避免传统的机械搅拌造成的藻体损伤及能源浪费，且更有利于溶解氧的溢出，有利于藻细胞更充分地吸收营养成分及光能；(3)由于采用内部光照，使光能利用率明显增高，特别由于管道气升连续循环方式，藻液有规律地反复进入受光区造成一定的明暗效应，更有利于微藻的生长与代谢；(4)由于采用外部热交换装置，避免了直接加热造成藻液局部过热而导致微藻死亡；(5)采用在线检测与计算机控制技术来进行自动控制，调节微藻生长的各种主要因素——如温度、光照度、pH值 The present invention over the prior art has the following advantages and beneficial effects: (1) Since the microalgae culture systems introduced into the magnetic field for processing by the biological effects of magnetic, can significantly increase the growth rate of the algae, increasing yield, and can improve the nutritional content of amino acids, proteins, polysaccharides and other components; (2) the use of a continuous cycle gas lift pipeline, result in stable uniform stirring effect can be avoided traditional mechanical stirring algae and energy waste caused by injury, and more DO favor overflow, algal cells more conducive to sufficiently absorb light energy and nutrients; (3) the use of an internal light, significantly increased utilization of light energy, especially since the air-lift pipe in a continuous loop, algae solution regular repeatedly entering the light receiving region of the shading effect caused by a certain, more conducive to the growth and metabolism of the microalgae; (4) the use of external heat exchange device, avoiding direct heating caused by local overheating caused by algae solution microalgae death; (5) using line detection and computer control techniques to automatic control, a variety of factors regulating the growth of algae - such as temperature, light intensity, pH, 溶解氧、细胞浓度等，实现微藻生产过程连续性及高细胞密度生产的自动优化控制；(6)实现微藻养殖与收获一化；(7)采用全封闭生产系统，减少了水分蒸发、减少了污染物及外界环境条件变化造成的影响，有利于微藻的高效纯种培养；(8)采用管道式，可不受地理环境影响，并具有高的光照面积与培养体积之比，有利于高细胞密度培养；(9)将本系统的除气室加挡板间隔以兼作贮液罐，可解决管道式光生物反应器培养体积小的缺陷问题，使其更适合实际生产的需要；(10)本生产系统结构简单、能耗低、易于扩大生产。 Dissolved oxygen concentration cell, automatic optimization control microalgae production process and a high cell density continuous production; and (6) achieve a microalgae culture and harvest of; (7) The closed production system, reducing the evaporation of water, reduces the impact of pollutants and environmental change of external conditions, contribute to the efficient culture of microalgae pure; (8) using the pipeline, can not affect the geographical environment, and has high illumination area to volume ratio of the culture, facilitate high cell density cultures; (9) the degassing chamber of the present system serves to increase the baffle spacing storage tank, pipeline resolves culture photobioreactor small volume defects, making it more suitable for practical production; ( 10) The production system is simple, low energy consumption, easy to expand production.
下面对说明书附图进一步说明如下：图1为管道气升式循环磁处理光生物反应器微藻生产系统的结构组成示意图，图2为本微藻生产系统的计算机监控原理框图，图3为其计算机监控程序流程图。 The following description of the accompanying drawings as further described below: FIG. 1 is a structural diagram of a magnetic conduit airlift bioreactor processing optical system microalgae, FIG. 2 is a computer monitoring system microalgae block diagram, FIG. 3 is a which computer program flowchart of the monitoring.
本发明的实施方式可为如下：(1)可按图1、图2所示，设计、加工、制造或选购本生产及控制系统的各组成部件。 Embodiments of the invention may be as follows: (1) 1, as shown, design, processing, manufacturing or various optional components of the present production and control system according to FIG. 2 FIG. 主要部件如：管道气升循环磁处理光生物反应器的气升室13可采用较细的透明玻璃管或塑料管制成；除气室17采用透明有机玻璃板用机加工方法加工制成；气降室36采用比气升室13较粗的透明玻璃管或塑料管制成；光源35采用日用荧光灯；恒定磁场可调装置14可采用可调电磁感应线圈或采用不同磁场强度的永久磁铁；热交换器16可采用实验室常用的控温水浴装置或其它通用控温装置；蠕动泵37可采用RDB--9型蠕动泵；空气压缩机4可采用永磁式ACO--777型；空气过滤器6可由活性类、玻璃丝等材料填制而成，回转过滤机表面材料可选如帆布等能让水分子透过的材料；计算机26可选用8098单片机；温度传感器20选用3TC--PT100型；pH电极21可选用E--201型复膜pH电极；溶氧电极28可采用DO--24型；浊度仪29及浊度传感器31可选用配套的LT300--LA型浊度仪；测光探头34可采用GK型光电 The main components: a magnetic loop air-lift pipe airlift bioreactor chamber processing optical glass or transparent plastic tubing 13 can be smaller; degassing chamber 17 using a transparent plexiglass plate formed by machining method of machining; gas drop chamber 13 thicker than 36 using a transparent glass or plastic chamber gas lift tubing; household fluorescent lamp using a light source 35; a constant magnetic field device 14 may employ an adjustable permanent magnet or an electromagnetic induction coil is adjustable with different magnetic field strength; thermal exchanger 16 can be commonly used laboratory temperature water bath temperature control means or other commonly used; peristaltic pump 37 may be employed RDB - 9 peristaltic pump; air compressor 4 can be permanent magnet ACO - 777 type; the air filter 6 may be an active type, made of glass wool and other fill material, the surface material may be selected rotary filter material such as canvas allows water molecules to pass through; optional computer 26 8098; 20 temperature sensor selection 3TC - PT100 type; pH electrode 21 optional E - 201 laminating type pH electrode; DO electrode 28 may be employed DO - 24 type; turbidity turbidity sensors 29 and 31 can be selected supporting LT300 - LA turbidimeter; measured GK optical probe 34 can be photoelectric 感器；A/D模数转换器40可选用AD7701型；D/A数模转换器41可选用AD667型12位D/A转换器；PIO转换器45可选用Z80--PIO型；(2)采用一般的机加工方法或制造现有光生物反应器的常规方法，加工制造本系统中的管道气升式磁处理光生物反应器的组成部件，然后按图1、图2所示，并按上面说明书所述的连接关系进行安装，便能较好地实现本微藻生产系统。 Sensor; A / D analog-digital converter 40 can be selected type AD7701; D / A analog converter 41 can be selected AD667 type 12-bit D / A converter; the PIO converter 45 can be selected Z80 - PIO type; (2 ) using the general method or a machining method for the conventional manufacture of conventional photobioreactor, the manufacturing components of the system in pipeline processing optical magnetic airlift bioreactor, and in Figure 1, Figure 2, and press installation according to the description above connection relationship, can better achieve this microalgae production systems. 然后按上面说明书所述的本生产系统的监控方法，并按图3所示的监控程序流程图编制软件程序，再结合上面说明书所述的本生产系统的作用原理和监控原理进行运行调试和试验，便能较好地实现本发明。 Then monitoring method according to the above description of the present production system, according to FIG. 3 shown in the flowchart monitor preparation software program, and then binding the above principle and the monitoring principle according to the present specification, the production system will be running the debug and test the present invention is able to achieve better.