KR20150006718A - Microalgal Cultivation Facility Using Closed Carbon Dioxide Photobioreactor - Google Patents

Microalgal Cultivation Facility Using Closed Carbon Dioxide Photobioreactor Download PDF

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KR20150006718A
KR20150006718A KR1020130080531A KR20130080531A KR20150006718A KR 20150006718 A KR20150006718 A KR 20150006718A KR 1020130080531 A KR1020130080531 A KR 1020130080531A KR 20130080531 A KR20130080531 A KR 20130080531A KR 20150006718 A KR20150006718 A KR 20150006718A
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South Korea
Prior art keywords
carbon dioxide
photobioreactor
concentration
air
closed
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KR1020130080531A
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Korean (ko)
Inventor
김근용
이계안
김기용
김성동
이치헌
윤지현
송지효
김광규
유찬효
박아영
이동훈
신명식
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(주)엔엘피
한국남부발전 주식회사
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Priority to KR1020130080531A priority Critical patent/KR20150006718A/en
Publication of KR20150006718A publication Critical patent/KR20150006718A/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/36—Means for collection or storage of gas; Gas holders
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/24—Recirculation of gas
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/06—Means for regulation, monitoring, measurement or control, e.g. flow regulation of illumination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/48—Automatic or computerized control
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/12—Unicellular algae; Culture media therefor

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  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
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  • General Health & Medical Sciences (AREA)
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  • Microbiology (AREA)
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  • General Engineering & Computer Science (AREA)
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  • Analytical Chemistry (AREA)
  • Computer Hardware Design (AREA)
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  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The present invention relates to a microalgal cultivation device using a closed carbon dioxide photobioreactor, and more specifically, to a microalgal cultivation device using a closed carbon dioxide photobioreactor designed for having a process in which carbon dioxide mixed with air is consistently circulated.

Description

BACKGROUND OF THE INVENTION 1. Field of the Invention [0001] The present invention relates to a closed-loop carbon dioxide photobioreactor (hereinafter referred to as " Closed Carbon Dioxide Photobioreactor &

The present invention relates to a microalgae culture apparatus using a closed carbon dioxide photobioreactor, and more particularly, to a culture apparatus using a closed-type carbon dioxide photobioreactor designed to have a process of continuously circulating carbon dioxide mixed with air, The present invention relates to an efficient microalgae culture apparatus capable of reducing the production cost as well as enhancing the biomass productivity when a microalgae is cultivated with a closed carbon dioxide photobioreactor because the consumption of carbon dioxide is lower than that of an open type photobioreactor.

In general, microalgae fix the carbon dioxide in the atmosphere through photosynthesis, and at the same time produce oxygen. Since the content of protein and lipid is high and contains amino acid and effective nutrients, the cultured microalgae culture itself contains It is possible to utilize directly as a food for aquaculture.

In addition, the dried biomass can be used as an animal feed, and after proper extraction, it is utilized as a high value-added natural material such as bio-fuels such as bioethanol, biodiesel, natural pigment, natural cosmetic raw materials, It is possible.

In mass production of such useful microalgae, the productivity is generally increased when air and carbon dioxide are mixed and used in an appropriate ratio.

However, in an open type photobioreactor, which is a widely used microalgae cultivation method, carbon dioxide mixed with air enters into a photobioreactor through an air hose and naturally diffuses into the atmosphere. As a result, the consumption of carbon dioxide is increased and the production cost of the microalgae biomass is increased, which makes it difficult to economically produce large capacity microalgae.

The present invention has been developed to solve the above-described problems of the related art. The apparatus for culturing microalgae using the closed carbon dioxide photobioreactor developed in the present invention prevents the carbon dioxide mixed with air from diffusing into the atmosphere and flowing out , And it is designed to have a process of continuously circulating while keeping the concentration of carbon dioxide mixed with the air constant, so that the consumption of carbon dioxide is much lower than that of an open type photo bioreactor. Therefore, The present invention aims to provide a microalgae culture apparatus using a closed carbon dioxide photobioreactor capable of simultaneously increasing the productivity of the biomass and reducing the production cost.

According to the present invention, the closed-type carbon dioxide photobioreactor is designed to continuously circulate carbon dioxide mixed with air, so that when the micro-algae are cultured as the consumption of carbon dioxide is low, the productivity of biomass and the reduction of production cost Can be expected at the same time.

In addition, since the closed-type carbon dioxide photobioreactor can utilize not only commercially available pure carbon dioxide but also flue gas containing carbon dioxide discharged from a power plant or the like, and carbon dioxide captured and mixed with air, Can be minimized.

In particular, the present invention relates to an efficient microalgae culture apparatus capable of increasing the microalgae culture efficiency and reducing the production cost when microalgae are cultured in a closed carbon dioxide photobioreactor because the consumption of carbon dioxide is low.

Brief Description of the Drawings Fig. 1 is an overall view of a microalgae culture apparatus using a closed carbon dioxide photobioreactor according to the present invention. Fig.
2 is a photograph showing an embodiment of a microalgae culture apparatus using a closed carbon dioxide photobioreactor of the present invention.

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS Hereinafter, a detailed description of the present invention will be described with reference to the accompanying drawings.

The embodiments of the present invention may be modified in various ways, and the scope of the present invention should not be construed as being limited by the embodiments described below.

1 is a block diagram of an apparatus for culturing microalgae using a closed carbon dioxide photobioreactor according to the present invention.

The microalgae culture apparatus using the closed carbon dioxide photobioreactor 10 of the present invention is a closed type photobioreactor. The microalgae culture medium inside the photobioreactor 10 is cultured by air and carbon dioxide, The remaining amount of carbon dioxide consumed in the microalgae culture together with the air is collected through the collection port 12 and the concentration of the collected carbon dioxide is measured by the carbon dioxide sensor 21 in the control unit 20, The air and the carbon dioxide are supplied to the photobioreactor 10 again by the air pump 31,

The concentration of carbon dioxide measured by the carbon dioxide sensor 21 and the concentration of carbon dioxide required for re-feeding the photobioreactor 10 are displayed in the concentration display and setting device 22,

When the concentration of carbon dioxide supplied to the photobioreactor 10 by the air pump 31 becomes lower than the concentration set in the carbon dioxide concentration indicating and setting device 22, the supply of the carbon dioxide in the carbon dioxide supplying unit 40 the carbon dioxide is regenerated through the solenoid valve 44 on / off to regulate the carbon dioxide concentration.

Finally, the closed type carbon dioxide photobioreactor 10 is repeatedly subjected to a series of processes to supply carbon dioxide. The present invention relates to a microalgae culture apparatus using a closed carbon dioxide photobioreactor.

Therefore, the components of the microalgae culture apparatus using the closed carbon dioxide photobioreactor will be described.

An inlet (11) into which carbon dioxide mixed with air is injected; And a collection port (12) for re-collecting air and carbon dioxide from the microalgae culture liquid, and a photobioreactor (10) manufactured in a closed structure

A carbon dioxide cylinder 41 for providing carbon dioxide; A regulator 42 for regulating the flow rate in accordance with the pressure in the carbon dioxide bomb; A carbon dioxide flow meter (43) for measuring the flow rate of carbon dioxide from the regulator; And a solenoid valve 44 for turning on / off the supply of carbon dioxide by the carbon dioxide concentration indicating and setting device 22. The carbon dioxide supply part 40 is connected to the photobioreactor 10,

A carbon dioxide sensor 21 for measuring the carbon dioxide re-collected from the collection port 12 of the photobioreactor 10; And a carbon dioxide concentration display and setting device (22) connected to the carbon dioxide sensor for displaying the concentration of carbon dioxide and turning on / off the solenoid valve according to the set concentration,

An air pump 31 connected to the control unit 20 and injecting carbon dioxide and air measured by the carbon dioxide sensor 21 into the photobioreactor 10; And an air flow meter (32) for measuring the flow rate of carbon dioxide and air,

And the carbon dioxide is continuously circulated. The present invention relates to a microalgae culture apparatus using a closed carbon dioxide photobioreactor.

FIG. 2 is a photograph showing an embodiment of a microalgae culture apparatus using the closed carbon dioxide photobioreactor of the present invention, and it can be applied to cultivation scales of at least 1 L level and culturing scales of up to 1 m 3 level.

Meanwhile, the microalgae cultivation through the closed-type carbon dioxide photobioreactor that has been researched and developed in the present invention can control the carbon dioxide concentration according to the cultivation operating conditions.

The carbon dioxide used in the microalgae culture apparatus through the closed-type carbon dioxide photobioreactor research and development developed in the present invention can supply pure carbon dioxide for industrial use by using a carbon dioxide bomb, but the carbon dioxide contained in the post- Or may be supplied using captured carbon dioxide.

10: photobioreactor 11: inlet
12: Collection port
20: control unit 21: carbon dioxide sensor
22: CO2 concentration display and setting device
30: air supply unit 31: air pump
32: Air flow meter
40: carbon dioxide supply part 41: carbon dioxide bombardment
42: regulator 43: carbon dioxide flow meter
44: Solenoid valve

Claims (3)

An inlet (11) into which carbon dioxide mixed with air is injected; And a collection port (12) for re-collecting air and carbon dioxide from the microalgae culture liquid, and a photobioreactor (10) manufactured in a closed structure

A carbon dioxide cylinder 41 for providing carbon dioxide; A regulator 42 for regulating the flow rate in accordance with the pressure in the carbon dioxide bomb; A carbon dioxide flow meter (43) for measuring the flow rate of carbon dioxide from the regulator; And a solenoid valve 44 for turning on / off the supply of carbon dioxide by the carbon dioxide concentration indicating and setting device 22. The carbon dioxide supply part 40 is connected to the photobioreactor 10,

A carbon dioxide sensor 21 for measuring the carbon dioxide re-collected from the collection port 12 of the photobioreactor 10; And a carbon dioxide concentration display and setting device 22 connected to the carbon dioxide sensor 21 for turning on / off the solenoid valve 44 according to the concentration of the carbon dioxide and the set concentration,

An air pump 31 connected to the control unit 20 for injecting carbon dioxide and air measured by the carbon dioxide sensor 21 into the photobioreactor 10; And an air supply unit (30) including an air flow meter (32) for measuring a flow rate of the carbon dioxide and air. The apparatus for culturing microalgae using the closed carbon dioxide photobioreactor according to claim 1,
The method according to claim 1,
The carbon dioxide concentration indicating and setting device 22 displays the carbon dioxide concentration measured by the carbon dioxide sensor 21 which measures the carbon dioxide re-collected from the trap 12 of the photobioreactor 10,
And to set the concentration of carbon dioxide necessary for re-supply to the photobioreactor (10). ≪ RTI ID = 0.0 > [10] < / RTI > The method according to claim 1,
When the concentration of carbon dioxide supplied to the photobioreactor 10 becomes lower than the concentration set in the carbon dioxide concentration indicating and setting device 22, the solenoid valve 44 supplies carbon dioxide to the air pump 31 ) To the photobioreactor (10). The apparatus for culturing microalgae using the closed carbon dioxide photobioreactor according to claim 1,
KR1020130080531A 2013-07-09 2013-07-09 Microalgal Cultivation Facility Using Closed Carbon Dioxide Photobioreactor KR20150006718A (en)

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20190030122A (en) * 2017-09-13 2019-03-21 한국해양과학기술원 Batch or continuous culture device comprising recycling loop and the culture method using thereof
WO2019087197A1 (en) * 2017-11-06 2019-05-09 Algaennovation Ltd. System and method of growing algae using geothermal gas
CN110387315A (en) * 2019-08-27 2019-10-29 济宁学院 Microalgae adsorbs the device and method of carbon dioxide preparation biodiesel

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20190030122A (en) * 2017-09-13 2019-03-21 한국해양과학기술원 Batch or continuous culture device comprising recycling loop and the culture method using thereof
WO2019087197A1 (en) * 2017-11-06 2019-05-09 Algaennovation Ltd. System and method of growing algae using geothermal gas
CN110387315A (en) * 2019-08-27 2019-10-29 济宁学院 Microalgae adsorbs the device and method of carbon dioxide preparation biodiesel

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