CN102533559B - Device and method for automatically feeding spirulina - Google Patents

Device and method for automatically feeding spirulina Download PDF

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Publication number
CN102533559B
CN102533559B CN201110459357.2A CN201110459357A CN102533559B CN 102533559 B CN102533559 B CN 102533559B CN 201110459357 A CN201110459357 A CN 201110459357A CN 102533559 B CN102533559 B CN 102533559B
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spirulina
index
turbidity
control system
detection
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CN102533559A (en
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骆其君
严小军
徐青山
谭应宏
彭友邻
殷丽川
屈桂华
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LIJIANG CHENGHAI BAO'ER BIOLOGICAL DEVELOPMENT Co.,Ltd.
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LIJIANG CHENGHAI BAO'ER BIOLOGICAL DEVELOPMENT Co Ltd
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00Bioreactors or fermenters specially adapted for specific uses
    • C12M21/02Photobioreactors
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/06Means for regulation, monitoring, measurement or control, e.g. flow regulation of illumination
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/26Means for regulation, monitoring, measurement or control, e.g. flow regulation of pH
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/30Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
    • C12M41/32Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of substances in solution
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/30Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
    • C12M41/36Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of biomass, e.g. colony counters or by turbidity measurements

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
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  • Bioinformatics & Cheminformatics (AREA)
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  • General Engineering & Computer Science (AREA)
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  • Microbiology (AREA)
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  • Cultivation Of Seaweed (AREA)
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Abstract

The invention discloses a device and a method for automatically feeding spirulina. The method comprises the following main technique steps of: screening ecological indexes of a culture pond, determining detection indexes, configuring a detection device and carrying out on-line detection, configuring a central control system, and executing operation, wherein the central control system comprises feeding back data, establishing criterion and outputting a procedure; screening the ecological indexes of the culture pond comprises five indexes such as a pH value, dissolved oxygen, chlorophyll, light density, turbidity and the like. The device and the method for automatically feeding spirulina, provided by the invention, can be beneficial to achievement of the standardized and refined production management of the spirulina.

Description

A kind of automatic breeding apparatus and method of spirulina
Technical field
The present invention relates to a kind of automatic breeding apparatus and method of spirulina.
Background technology
Spirulina is produced through the development of two more than ten years, and the specification production model of unified intensive, stdn, automatization is conducive to the lifting of whole industry.Centralized the production of the spirulina GAP production standard according to pharmaceutical raw material is carried out to management in the world, this is the key of guaranteeing quality product, is also the important directions developing at present; And the regulation and control that become more meticulous of production process have become the key link of improving the quality of products with economic benefit.
It is CN1426678 that the patent of automatic breeding aspect has publication number, title " indoor automatic breeding system ", disclose for cultivation biological cultivation is cultivated in indoor stepped culturing pool employing stage, make cultivation density increase and reduce space, and at any time with the water quality condition of water quality monitoring system monitoring culturing pool, make breeding water keep best state, can improve cultivation survival rate, increase cultured output.Publication number is CN1864474, name is called the patent of invention of " a kind of cultural method of spirulina ", discloses the cultural method of a kind of spirulina breeding barrel in booth, adopts enclosed industrial method cultivation, can Artificial Control solution temperature and light application time, improve output.
But current production process regulation and control, still rely on and observation and the operation of total man's work, lack the application of relevant electromechanical controlling device and automatic breeding.Traditional spirulina still adopts extensive style cultivation, integral production inefficiency.Cultivating spirulina footprint area is large, and industrial scale often reaches hundreds thousand of square metres, and artificial labour intensity is large.Particularly, for being that continuation is cultivated or gathering to lack to supply the method and apparatus of discriminating, aging spirulina is cultivated in continuation often, and so, the speed of growth is low, produces and there is no benefit; If by not yet arriving or in the early stage spirulina gather ahead of time of exponential growth, wasting to cultivate directly and drop into, do not obtain due benefit.Visible, the automatization of production technique is the important guarantee that reduces labor intensity, improves productivity effect.
Along with the widespread use of mechanize, automatization, particularly plant industry for the breeding of traditional agriculture, become and led industry forward position.By mechanize day by day replace artificial operation, make up labor force's wretched insufficiency; Reduce artificial error, reaching becomes more meticulous produces and management; Reduce manual operations, reduce the safety problem that directly has influence on product.Cultivation automatization is also the important symbol of this lifting industry.
Summary of the invention
The invention provides a kind of apparatus and method of automatic breeding of spirulina, solve the technical problem of above-mentioned existence.
The present invention be according to " screening culturing pool ecological index---determine and detect index---configuration of test set---detect online---comprise data feedback, set up the central control system of criterion and program output---executable operations " mathematical logic realize its technical scheme.Technical scheme is as follows:
An automatic breeding method for spirulina, main technique link comprises:
Screening culturing pool ecological index, the configuration of determining detection index, test set and online detection, master control system and executable operations link; Described master control system by data feedback, set up criterion and program is exported;
Described screening culturing pool ecological index comprises the five large indexs such as pH value, dissolved oxygen, chlorophyll, optical density(OD), turbidity.
Described regular inspection is really surveyed index: adopt YSI6600 Multifunctional water to detect instrument and carry out on-line determination pH value, dissolved oxygen, chlorophyll and turbidity at screening culturing pool; Adopt 721 spectrophotometric determination optical density(OD); Detected result and growth of spirulina platensis situation are compared, judge that the spirulina cultivated is whether in the exponential growth later stage and reached certain density, in view of the high correlation of turbidity and spirulina density, determine turbidity as whether to the detection index of collection period, the data and the spectrophotometer method that detect are compared, reach the scope that optical density(OD) is 0.75-0.85, be equivalent to according to 1 ton of nutrient solution, at every turn can output spirulina powder 0.9-1.05kg as comparison.
The operation of described master control system: arrive master control system by data feedback, set up criterion, the data of detection and culturing pool ecological index are compared, form different instructions.
Described executable operations is: if turbidity reaches the index of setting, belong under normal growth conditions, feedback information is exported, open in time the valve of gathering, start the algae liquid of cultivation pool to enter between collecting cart by pipeline, after having gathered, add in time nutrient solution, increase water level, enter next production cycle flow process; If turbidity does not change or can not reach the index of setting, belong under abnormal growth conditions, further gather the sample of cultivation pool, Source Tracing, changes algae kind or nutrient solution in time, regulates culture condition.
The present invention be conducive to realize spirulina standardization, production management becomes more meticulous.
Brief description of the drawings
Fig. 1 is the automatic breeding apparatus and method schematic diagram of a kind of spirulina of the present invention.
Embodiment
embodiment 1
1, in experiment pool, screening and mensuration culturing pool ecological index: screening culturing pool ecological index comprises the five large indexs such as pH value, dissolved oxygen, chlorophyll, optical density(OD), turbidity.
2, determine and detect index: detected result and growth of spirulina platensis situation are compared, judge that the spirulina cultivated is whether in the exponential growth later stage and reached certain density, in view of the high correlation of turbidity and spirulina density, determine turbidity as whether to the detection index of collection period.The data and the spectrophotometer method that detect are compared, reach the scope that optical density(OD) is 0.75-0.85, be equivalent to according to 1 ton of nutrient solution, can output spirulina powder 0.9-1.05kg (it be 3.0 days according to the interval of gathering as comparison at every turn, be equivalent to 120 times/year, year output reaches 108-126kg/year so).
3, first in experiment pool, the configuration of test set detects with online: the online automatic detection device that adopts turbidity that detects, adopts YSI6600 Multifunctional water to detect instrument on-line determination pH value, dissolved oxygen, chlorophyll, turbidity; Adopt 721 spectrophotometric determination optical density(OD).
4, the operation of master control system: arrive master control system by data feedback, set up criterion, the data of detection and culturing pool ecological index are compared.
5, executable operations: if belonged under normal growth conditions, the index that reaches setting of turbidity, feedback information is exported, open in time the valve of gathering, start the algae liquid of cultivation pool to enter between collecting cart by pipeline, after having gathered, add in time nutrient solution, increase water level, enter next production cycle flow process; If turbidity does not change or can not reach the index of setting, belong under abnormal growth conditions, further gather the sample of cultivation pool, Source Tracing, changes algae kind or nutrient solution in time, regulates culture condition.

Claims (1)

1. an automatic breeding method for spirulina, is characterized in that main technique link comprises:
Screening culturing pool ecological index, the configuration of determining detection index, test set and online detection, master control system and executable operations link; Described master control system by data feedback, set up criterion and program is exported,
Described screening culturing pool ecological index is pH value, dissolved oxygen, chlorophyll, optical density(OD) and the large index of turbidity five;
Described regular inspection is really surveyed index: adopt YSI6600 Multifunctional water to detect instrument and carry out on-line determination pH value, dissolved oxygen, chlorophyll and turbidity at screening culturing pool; Adopt 721 spectrophotometric determination optical density(OD); Detected result and growth of spirulina platensis situation are compared, judge that the spirulina cultivated is whether in the exponential growth later stage and reached certain density, in view of the high correlation of turbidity and spirulina density, determine turbidity as whether to the detection index of collection period, the data and the spectrophotometer method that detect are compared, reach the scope that optical density(OD) is 0.75-0.85, be equivalent to according to 1 ton of nutrient solution, at every turn can output spirulina powder 0.9-1.05kg as comparison;
Operating to of described master control system: arrive master control system by data feedback, set up criterion, the data of detection and culturing pool ecological index are compared, form different instructions;
Described executable operations is: if turbidity reaches the index of setting, belong under normal growth conditions, feedback information is exported, open in time the valve of gathering, start the algae liquid of cultivation pool to enter between collecting cart by pipeline, after having gathered, add in time nutrient solution, increase water level, enter next production cycle flow process; If turbidity does not change or can not reach the index of setting, belong under abnormal growth conditions, further gather the sample of cultivation pool, Source Tracing, changes algae kind or nutrient solution in time, regulates culture condition.
CN201110459357.2A 2011-12-31 2011-12-31 Device and method for automatically feeding spirulina Active CN102533559B (en)

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Publication number Priority date Publication date Assignee Title
CN103555570A (en) * 2013-10-31 2014-02-05 中盐制盐工程技术研究院 Brand-new industrial continuous efficient culture system of dunaliella salina
CN105018329A (en) * 2014-04-15 2015-11-04 东营大振生物科技有限公司 Spirulina harvest equipment
ES2633027B2 (en) * 2016-03-18 2018-04-20 José TELLO RIPA Industrial module for intensive cultivation of microalgae with controls for lighting, temperature, CO2, agitation and trace elements
CN105861364A (en) * 2016-04-15 2016-08-17 内蒙古乌审召生态产业发展有限公司 Automatic culture method and system for spirulina
CN107900278B (en) * 2017-10-11 2020-07-03 武汉纺织大学 Automatic control method for biological regeneration of water glass used sand bloom
TWI690262B (en) * 2018-12-25 2020-04-11 國立臺灣海洋大學 The method for large scale culturing of noctiluca scintillans

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1377955A (en) * 2002-05-28 2002-11-06 李恒光 Fresh edible micro algae composite cultivating source and its use
CN201615902U (en) * 2009-12-16 2010-10-27 中国科学院水生生物研究所 Bloom warning system
CN101900718A (en) * 2010-05-06 2010-12-01 中国科学院上海微系统与信息技术研究所 Store-and-forward node for monitoring lake water quality

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1377955A (en) * 2002-05-28 2002-11-06 李恒光 Fresh edible micro algae composite cultivating source and its use
CN201615902U (en) * 2009-12-16 2010-10-27 中国科学院水生生物研究所 Bloom warning system
CN101900718A (en) * 2010-05-06 2010-12-01 中国科学院上海微系统与信息技术研究所 Store-and-forward node for monitoring lake water quality

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Address before: 650000 No. 5 unit 2, B District, Jinzhou Bay, Panlong Bay, Panlong District, Yunnan, Kunming, 503

Patentee before: Yunnan Long Yang venture capital partnership (L.P.)