CN104498580B - The assay method of a kind of spirulina algae kind resistant to mechanical damage and recovery capability and application - Google Patents
The assay method of a kind of spirulina algae kind resistant to mechanical damage and recovery capability and application Download PDFInfo
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Abstract
The invention provides a kind of spirulina algae kind resistant to mechanical damage and be mechanically damaged assay method and the application of rear recovery capability, comprising the following steps: 1) spirulina algae kind 3～40L to be measured is provided, the average length X of frond before mensuration mechanical damage1；2) algae seedling solution is carried out mechanical damage process；3) the average length X of frond after mensuration mechanical damage2；4) will through step 2) algae seedling solution that processes cultivates 2～6 days, measures algae body average length after cultivating, the average length X of frond after recovering as mechanical damage3；5) the resistant to mechanical damage ability value=(X of algae kind is calculated2/X1) × 100%.6) what calculate algae kind to be measured is mechanically damaged rear recovery capability value=(X2/X3) × 100%.The inventive method can be applicable to the screening of spirulina algae kind, shortens cycle and the cost of screening, for obtaining the spirulina production and operation offer reliable guarantee of high-quality, high yield, low cost.
Description
Technical field
The invention belongs to technical field of aquaculture, be specifically related to the cultural technique of spiral algae, particularly relate to the triage techniques of spirulina algae kind.
Background technology
Spirulina (Spirulina) is Cyanophyta (Cyanophyta) Oscillatoriales (Oscillatoriales) Oscillariaceae (Oscillatoriaceae) Spirullina (Spirulina), and the spirulina algae kind of artificial cultivation mainly has two kinds both at home and abroad at present: spirulina plalensis (S.platensis) and spirulina maxim (S.maxima).Because the microalgae long-term cultivation such as spirulina are in non-natural environment, the group of algae kind can be made to morph or degenerate, even pollute other unexpected algae kinds.Therefore, constantly separate, cultivate, screen the spirulina algae kind being suitable for large-scale farming, be realize the essential condition that the sustainable industrialization of spirulina produces.
Spirulina is bred with fracture mode, namely forms hormocystangium after the fracture of single frond, and it is individual that hormocystangium is further continued for being grown to serve as complete spirulina.In the process of industrialization cultivating spirulina, gather using water pump, change the process procedures such as water, all spirulina can be caused mechanical damage artificially, particularly when gathering, algae solution is after water pump extracting, spirulina frond can be subject to the mechanism of external force and rupture, post-rift spirulina frond usually returns to original form after outgrowth, but after long-term external mechanical influence repeatedly, part frond can shorten gradually, become broken, formed and original spirulina frond form, the frond that architectural difference is bigger, it is unfavorable for the continued propagation of spirulina and gathers, affect yield and the quality of industrialized aquaculture spirulina, thus directly influencing the benefit of spirulina industrialization cultivation.Therefore, screening has the spirulina algae kind of enough resistant to mechanical damage abilities and recovery capability, is the key of spirulina industrialized aquaculture, is also one of key factor of must take in Strain selection process.
Screening technique currently for spirulina algae kind is to be extended in outdoor breeding pond step by step by algae kind to carry out pilot scale cultivation (500m2Above cultured area), in pilot scale incubation, the index such as the morphological stability of spirulina and yield is as the foundation judging that algae kind is good and bad.Algae kind is cultivated indoor and is started to completing whole outdoor pilot scale breeding process at least to need 60 days, and each test at least needs algae seedling solution 100L, and is subject to extraneous factor interference in incubation, affects the accuracy of evaluation result.For needing the spirulina industrialized aquaculture enterprise of Large-scale Screening spirulina algae kind, the method cycle length, efficiency is low, cost is high, poor accuracy.
Summary of the invention
Deficiency for existing spirulina Strain selection method, it is an object of the invention to provide a kind of convenient, fast, accurate, directly perceived, cost is low, and suitable in industrialization cultivating spirulina, measure algae kind resistant to mechanical damage and be mechanically damaged method and the application thereof of rear recovery capability.
The invention provides a kind of spirulina algae kind resistant to mechanical damage and be mechanically damaged the assay method of rear recovery capability, comprising the following steps:
1) provide certain density spirulina algae seedling solution 3～40L to be measured, measure the average length of frond in algae seedling solution, as the average length X of spirulina frond before mechanical damage1；
2) spirulina algae kind to be measured carries out mechanical damage respectively artificially process；
3) average length of frond in the algae seedling solution that mechanical damage processes is measured, as the average length X of mechanical damage rear screw algae frond2；
4) will through step 2) the spirulina algae seedling solution that processes continues cultivation 2～6 days, and then measure the average length of frond in the algae seedling solution of recovered cultivation, recover the average length X of rear screw algae frond as mechanical damage3；
5) the resistant to mechanical damage ability value of spirulina algae kind to be measured, described resistant to mechanical damage ability value=(X are calculated2/X1) × 100%；
6) what calculate spirulina algae kind to be measured is mechanically damaged rear recovery capability value, described in be mechanically damaged rear recovery capability value=(X2/X3) × 100%.
According in one embodiment of the invention, described step 1) through the following steps that realize: random taking-up spirulina frond 10～30 in certain density spirulina algae seedling solution to be measured, to be measured microscopically frond length with eyepiece micrometer, calculate meansigma methods, as the average length X of spirulina frond before mechanical damage1。
According in one embodiment of the invention, described step 3) through the following steps that realize: from through step 2) the spirulina algae seedling solution that processes random take out machinery injury region reason after frond 10～30, to be measured microscopically frond length with eyepiece micrometer, calculate meansigma methods, as the average length X of mechanical damage rear screw algae frond2。
According in one embodiment of the invention, described step 4) through the following steps that realize: will through step 2) the spirulina algae seedling solution renewal cultivation that processes 2～6 days, then the frond 10～30 after renewal cultivation is taken out at random, to be measured microscopically frond length with eyepiece micrometer, calculate meansigma methods, as the average length X of renewal cultivation rear screw algae frond3。
According in one embodiment of the invention, step 2) described in mechanical damage process in mechanical damage analog realize, described mechanical damage analog include for contain spirulina algae seedling solution can hermetic container, and be fixed on the single-phase submersible pump in described container；It is process described algae seedling solution 3～10min by submersible pump with the condition of voltage 220V, power 0.20～0.60KW, rotating speed 1500～4000r/min, lift 5～25m to realize that described mechanical damage processes；Preferably, described can the box with cover of hermetic container to be volume be 5～50L；It is more preferably the pulley-type box with cover of 5～50L.
According in one embodiment of the invention, it is process described algae seedling solution 3～6min by submersible pump with the condition of voltage 220V, power 0.20～0.60KW, rotating speed 1500～3000r/min, lift 5～15m to realize that described mechanical damage processes.
According in one embodiment of the invention, the concentration of described spirulina algae seedling solution measures what the optical density value that described spirulina algae seedling solution obtains represented with ultraviolet spectrophotometer under 560nm, hereafter with OD560nmValue represents.Preferably, the OD of described spirulina algae seedling solution560nmValue is 0.2～1.0.
On the other hand, present invention also offers said method application in spirulina Strain selection: provide two or more OD560nmIt is worth identical spirulina algae seedling solution to be measured, obtained the resistant to mechanical damage ability value of each algae kind to be measured by said method respectively and be mechanically damaged rear recovery capability value, then by the resistant to mechanical damage ability value of each algae kind be mechanically damaged rear recovery capability value and carry out the comparison between algae kind, to determine high-quality algae kind；Wherein, the ability value of algae kind resistant to mechanical damage is more big, illustrates that algae kind resistant to mechanical damage ability is more strong；It is more little that algae kind is mechanically damaged rear recovery capability value, illustrates that the mechanically damaged rear recovery capability of algae kind is more strong.
According in one embodiment of the invention, spirulina algae kind to be screened selects the ability value of resistant to mechanical damage relatively big, and be mechanically damaged the less algae kind of rear recovery capability value as resistant to mechanical damage and the recovery capability strong spirulina kind large-scale culture for spirulina.
According in one embodiment of the invention, when the spirulina algae kind that the spirulina algae kind that resistant to mechanical damage ability value is bigger is less with being mechanically damaged rear recovery capability value is not same spirulina algae kind, select spirulina algae kind that wherein resistant to mechanical damage ability value is bigger for the large-scale culture of spirulina.
The dynamics of mechanical damage and degree in the invention simulation actual production, test using a small amount of spirulina algae seedling solution as subjects, ability value and algae kind according to algae kind resistant to mechanical damage are mechanically damaged rear recovery capability value quantitatively as evaluation index, judge resistant to mechanical damage and the recovery capability of algae kind, with evaluate it can the probability of large-scale culture.And method 2-6 days of the present invention can obtain result, the test period is short, and spirulina algae seedling solution consumption only needs 3～40L, and input cost is low.Suitable in industrialization cultivating spirulina, screen resistant to mechanical damage and the strong high-quality spirulina algae kind of recovery capability, eliminate the algae kind being not suitable for large-scale cultivation in time.Relative to existing spirulina Strain selection method, the present invention provide a kind of convenient, fast, accurate, intuitively, measure the assay method of spirulina algae kind resistant to mechanical damage ability and mechanically damaged rear recovery capability at low cost, and it is applied in the screening of spirulina algae kind, shorten the cycle of spirulina Strain selection significantly, and decrease screening cost, for obtaining the spirulina production and operation offer reliable guarantee of high-quality, high yield, low cost.
Specific embodiment
For explanation the object, technical solutions and advantages of the present invention clearly, further illustrate the present invention below in conjunction with embodiment.Specific embodiment described herein, only in order to the description and interpretation present invention, is not the application category limiting the present invention.
Embodiment 1:
1#～4# spirulina algae kind carrying out resistant to mechanical damage and is mechanically damaged rear recovery capability mensuration, therefrom screening is suitable for the spirulina algae kind of large-scale farming, and test result is verified.Concrete grammar is as follows:
30L pulley-type box with cover 4, wherein respectively puts into single-phase submersible pump 1, fixing.1#～4# spirulina algae kind respectively takes OD560nmThe algae seedling solution 20L of=0.5, respectively takes frond 10 respectively from algae seedling solution to be measured, by its frond length of measurement microscope with eyepiece micrometer, calculates meansigma methods, is calculated as the average length X of spirulina frond before mechanical damage1.The algae seedling solution of each spirulina algae kind to be measured is respectively put in pulley-type box, seals, and opens submersible pump, power: 0.20KW, rotating speed: 1500r/min, lift: 5m, voltage: 220V, processes time: 3min.After mechanical damage processes, then take out each algae body 10 after damage processes respectively, by its frond length of measurement microscope with eyepiece micrometer, calculate meansigma methods, be calculated as the average length X of mechanical damage rear screw algae frond2.Algae seedling solution after mechanical damage is processed takes each algae body 10 after continuing cultivation 2 days respectively, by its frond length of measurement microscope with eyepiece micrometer, calculates meansigma methods, is calculated as mechanical damage and recovers the average length X of rear screw algae frond3.Test result is as shown in table 1.
The resistant to mechanical damage of table 11#～4# spirulina algae kind and be mechanically damaged rear recovery capability measurement result
| Algae kind is numbered | 1# | 2# | 3# | 4# |
| X1(μm) | 285.3 | 272.4 | 321.5 | 340.7 |
| X2(μm) | 175.2 | 223.4 | 210.6 | 185.3 |
| X3(μm) | 306.3 | 355.7 | 216.2 | 263.6 |
| Algae kind resistant to mechanical damage ability value (%) | 61.4 | 82.0 | 65.5 | 54.4 |
| The mechanically damaged rear recovery capability value (%) of algae kind | 57.2 | 62.8 | 97.4 | 70.3 |
According to evaluation methodology of the present invention, in spirulina algae kind to be measured for 1#～4#, 2# algae kind resistant to mechanical damage ability is relatively strong, and it is stronger that 1# algae kind is mechanically damaged rear recovery capability.When after resistant to mechanical damage ability value and damage, the evaluation result of recovery capability value is inconsistent, plant using the algae kind that resistant to mechanical damage ability value is big as large-scale culture.Thus may determine that 2# spirulina algae kind is more suitable for large-scale farming in spirulina algae kind to be measured for 1#～4#.
For this result is verified, 1#～4# spirulina algae kind is expanded cultivation by the present inventor further step by step, compare these four kinds of spirulina algae kinds morphological stability in actual breeding process and volume variance situation, to filter out, in 1#～4# spirulina algae kind, the algae kind being more suitable for large-scale farming.When morphological stability is with initial frond length and gathers, the difference of frond length is weighed, and difference more little explanation frond form in breeding process is more stable, otherwise, then frond morphological stability in cultivation is poor.Method particularly includes: by 1#～4# spirulina algae kind OD560nmThe each 100L difference expansion step by step of algae seedling solution of=0.5 cultivates to cultured area 500 square meter, amount to cultivation 60 days, during cultivation, every 6 days sampling determinations are once, randomly choose each spirulina frond 15 every time and carry out length measurment, frond length value is represented with meansigma methods, finally gather simultaneously, calculate yield.Test result is as shown in table 2.
The morphological stability of table 21#～4# spirulina algae kind pilot scale cultivation and volume variance situation
As can be known from Table 2, the initial frond length of 2# spirulina algae kind is minimum with the difference of frond length when gathering, and illustrates that 2# spirulina algae kind frond form in breeding process in 4 the spirulina algae kinds surveyed is the most stable；And the single rate in 4 the spirulina algae kinds surveyed is the highest after the cultivation of 2# spirulina algae kind.In conjunction with two as a result, it is possible to judge that in 4 spirulina algae kinds detected, 2# spirulina algae kind is best suited for large-scale farming.Consistent with the inventive method result of determination.
Embodiment 2:
5#～7# spirulina algae kind is carried out resistant to mechanical damage and is mechanically damaged rear recovery capability mensuration, and therefrom screening is suitable for the spirulina algae kind of large-scale farming.Concrete grammar is as follows:
5L pulley-type box with cover 3, wherein respectively puts into single-phase submersible pump 1, fixing.5#～7# spirulina algae kind respectively takes OD560nmThe algae seedling solution 3L of=0.2, therefrom takes each algae body 10 respectively, by its frond length of measurement microscope with eyepiece micrometer, calculates meansigma methods, is calculated as the average length X of spirulina frond before mechanical damage1.The algae seedling solution of algae kind to be measured is respectively put in pulley-type box with cover, seals, and opens submersible pump, power: 0.20KW, rotating speed: 1500r/min, lift: 5m, voltage: 220V, time: 5min.After mechanical damage processes, more respectively taking-up process after each algae body 10, by its frond length of measurement microscope with eyepiece micrometer, calculate meansigma methods, be calculated as the average length X of mechanical damage rear screw algae frond2.After algae seedling solution after mechanical damage is continued cultivation 2 days, take each algae body 10 respectively, by its frond length of measurement microscope with eyepiece micrometer, calculate meansigma methods, be calculated as mechanical damage and recover the average length X of rear screw algae frond3。
The resistant to mechanical damage of table 35#～7# algae kind and be mechanically damaged rear recovery capability measurement result
| Algae kind is numbered | 5# | 6# | 7# |
| X1(μm) | 321.0 | 319.4 | 310.6 |
| X2(μm) | 153.4 | 221.5 | 110.8 |
| X3(μm) | 163.8 | 258.4 | 116.5 |
| Algae kind resistant to mechanical damage ability value (%) | 47.8 | 69.3 | 35.7 |
| The mechanically damaged rear recovery capability value (%) of algae kind | 93.6 | 85.7 | 95.1 |
As shown in table 3, in tri-spirulina algae kinds of 5#～7#, 6# spirulina algae kind resistant to mechanical damage ability is relatively strong, and after mechanical damage, recovery capability is also stronger.Therefore, in spirulina algae kind to be measured for 5#～7#, 6# spirulina algae kind large-scale farming preferably.
Embodiment 3:
8#～12# algae kind is carried out resistant to mechanical damage and is mechanically damaged rear recovery capability mensuration, and therefrom screening is suitable for the algae kind of large-scale farming.Concrete grammar is as follows:
50L pulley-type box with cover 5, wherein respectively puts into single-phase submersible pump 1, fixing.8#～12# spirulina algae kind respectively takes OD560nmThe algae seedling solution 35L of=0.8, therefrom takes each algae body 15 respectively, by its frond length of measurement microscope with eyepiece micrometer, calculates meansigma methods, is calculated as the average length X of spirulina frond before mechanical damage1.The algae seedling solution of algae kind to be measured is respectively put in pulley-type box, seals, and opens submersible pump, power: 0.50KW, rotating speed: 3000r/min, lift: 10m, voltage: 220V, time: 8min.After mechanical damage processes, then take out each algae body 15 after damage processes respectively, by its frond length of measurement microscope with eyepiece micrometer, calculate meansigma methods, be calculated as the average length X of mechanical damage rear screw algae frond2.After algae seedling solution after mechanical damage is continued cultivation 5 days, take each algae body 15 respectively, by its frond length of measurement microscope with eyepiece micrometer, calculate meansigma methods, be calculated as mechanical damage and recover the average length X of rear screw algae frond3。
The resistant to mechanical damage of table 48#～12# algae kind and be mechanically damaged rear recovery capability measurement result
| Algae kind is numbered | 8# | 9# | 10# | 11# | 12# |
| X1(μm) | 297.4 | 317.3 | 328.9 | 335.7 | 283.8 |
| X2(μm) | 114.5 | 106.7 | 124.8 | 178.0 | 102.2 |
| X3(μm) | 143.2 | 125.8 | 144.5 | 276.1 | 135.2 |
| Resistant to mechanical damage ability value (%) | 38.5 | 28.5 | 37.9 | 55.5 | 36.0 |
| Mechanically damaged rear recovery capability value (%) | 80.0 | 71.9 | 86.4 | 67.5 | 75.6 |
As shown in table 4, in five spirulina algae kinds of 8#～12#, 11# spirulina algae kind resistant to mechanical damage ability is relatively strong, and after mechanical damage, recovery capability is also stronger.Therefore, in five spirulina algae kinds of 8#～12#, 11# spirulina algae kind large-scale farming preferably.
Embodiment 4:
13#～18# algae kind is carried out resistant to mechanical damage and is mechanically damaged rear recovery capability mensuration, and therefrom screening is suitable for the spirulina algae kind of large-scale farming.Concrete grammar is as follows:
40L pulley-type box 6, wherein respectively puts into single-phase submersible pump 1, fixing.13#～18# spirulina algae kind respectively takes OD560nmThe algae seedling solution 35L of=1.0, therefrom takes each algae body 30 respectively, by its frond length of measurement microscope with eyepiece micrometer, calculates meansigma methods, is calculated as frond average length (X before mechanical damage1).The algae seedling solution of algae kind to be measured is respectively put in pulley-type box, seals, and opens submersible pump, power: 0.60KW, rotating speed: 4000r/min, lift: 25m, voltage: 220V, time: 10min.After mechanical damage processes, more respectively taking-up process after each algae body 30, by its frond length of measurement microscope with eyepiece micrometer, calculate meansigma methods, be calculated as the average length X of mechanical damage rear screw algae frond2.After algae seedling solution after mechanical damage is continued cultivation 6 days, take each algae body 30 respectively, by its frond length of measurement microscope with eyepiece micrometer, calculate meansigma methods, be calculated as mechanical damage and recover the average length X of rear screw algae frond3。
The resistant to mechanical damage of table 513#～18# algae kind and be mechanically damaged rear recovery capability measurement result
| Algae kind is numbered | 13# | 14# | 15# | 16# | 17# | 18# |
| X1(μm) | 334.2 | 357.4 | 198.5 | 228.7 | 296.5 | 268.9 |
| X2(μm) | 257.3 | 276.4 | 100.5 | 101.4 | 98.4 | 113.5 |
| X3(μm) | 280.1 | 328.1 | 109.4 | 118.2 | 105.6 | 132.5 |
| Resistant to mechanical damage ability value (%) | 77.0 | 77.3 | 50.6 | 44.3 | 33.2 | 42.2 |
| Mechanically damaged rear recovery capability value (%) | 91.9 | 84.2 | 91.9 | 85.8 | 93.2 | 85.7 |
As shown in table 5, in six spirulina algae kinds of 13#～18#, 14# spirulina algae kind resistant to mechanical damage ability is relatively strong, and after mechanical damage, recovery capability is also stronger.Prompting: in six spirulina algae kinds of 13#～18#, 14# spirulina algae kind conveniently large-scale farming.
Although the specific embodiment of the present invention being described and illustrated in detail, it should be recognized that the present invention is not by the restriction of described detailed description of the invention.Without departing from the spirit and scope of the present invention, it is possible to the present invention is made various improvement, modification and change, and these improve, modify and change all within the scope of the present invention.
Claims (13)
1. spirulina algae kind resistant to mechanical damage and the assay method being mechanically damaged rear recovery capability, comprises the following steps:
1) provide certain density spirulina algae seedling solution 3～40L to be measured, measure the average length of frond in algae seedling solution, as the average length X of spirulina frond before mechanical damage1；
2) spirulina algae kind to be measured carries out mechanical damage artificially process；
3) average length of frond in the algae seedling solution that mechanical damage processes is measured, as the average length X of mechanical damage rear screw algae frond2；
4) will through step 2) the spirulina algae seedling solution that processes continues cultivation 2～6 days, and then measure the average length of frond in the algae seedling solution of recovered cultivation, recover the average length X of rear screw algae frond as mechanical damage3；
5) the resistant to mechanical damage ability value of spirulina algae kind to be measured, described resistant to mechanical damage ability value=(X are calculated2/X1) × 100%；
6) what calculate spirulina algae kind to be measured is mechanically damaged rear recovery capability value, described in be mechanically damaged rear recovery capability value=(X2/X3) × 100%.
2. the method for claim 1, it is characterized in that, described step 1) through the following steps that realize: random taking-up frond 10～30 in certain density spirulina algae seedling solution to be measured, to be measured microscopically frond length with eyepiece micrometer, calculate meansigma methods, as the average length X of spirulina frond before mechanical damage1。
3. the method for claim 1, it is characterized in that, described step 3) through the following steps that realize: from through step 2) the spirulina algae kind that processes random take out machinery injury region reason after frond 10～30, to be measured microscopically the length of frond after mechanical damage processes with eyepiece micrometer, calculate meansigma methods, as the average length X of mechanical damage rear screw algae frond2。
4. the method for claim 1, it is characterized in that, described step 4) through the following steps that realize: will through step 2) the spirulina algae seedling solution renewal cultivation that processes 2～6 days, then the frond 10～30 after renewal cultivation is taken out at random, with the length being measured microscopically the frond after renewal cultivation with eyepiece micrometer, calculate meansigma methods, as the average length X of the spirulina frond after renewal cultivation3。
5. the method for claim 1, it is characterized in that, described step 2) described in mechanical damage process in mechanical damage analog realize, described mechanical damage analog include for contain spirulina algae seedling solution can hermetic container, and be fixed on the single-phase submersible pump in described container；It is process described algae seedling solution 3～10min by submersible pump with the condition of voltage 220V, power 0.20～0.60KW, rotating speed 1500～4000r/min, lift 5～25m to realize that described mechanical damage processes.
6. method as claimed in claim 5, it is characterised in that described can hermetic container be the box with cover of volume 5～50L.
7. method as claimed in claim 5, it is characterised in that described can hermetic container be the pulley-type box with cover of 5～50L.
8. method as claimed in claim 5, it is characterised in that it is process described algae seedling solution 3～6min by submersible pump with the condition of voltage 220V, power 0.20～0.60KW, rotating speed 1500～3000r/min, lift 5～15m to realize that described mechanical damage processes.
9. the method as according to any one of claim 1～8, it is characterised in that the concentration of described spirulina algae seedling solution measures optical density value and the OD of described spirulina algae seedling solution with ultraviolet spectrophotometer under 560nm560nmValue represents.
10. method as claimed in claim 9, it is characterised in that the OD of described spirulina algae seedling solution to be measured560nmValue is 0.2～1.0.
11. the application that the method as according to any one of claim 1～10 is in spirulina Strain selection, it is characterised in that two or more OD is provided560nmIt is worth identical spirulina algae seedling solution to be measured, obtain the resistant to mechanical damage ability value of each algae kind to be measured respectively by the method according to any one of claim 1～10 and be mechanically damaged rear recovery capability value, by the resistant to mechanical damage ability value of each algae kind be mechanically damaged rear recovery capability value and carry out the comparison between algae kind respectively, to determine high-quality algae kind；Wherein, the ability value of algae kind resistant to mechanical damage is more big, illustrates that algae kind resistant to mechanical damage ability is more strong；It is more little that algae kind is mechanically damaged rear recovery capability value, illustrates that mechanically damaged rear recovery capability is more strong.
12. apply as claimed in claim 11, it is characterized in that, algae kind to be screened selects the ability value of resistant to mechanical damage relatively big, and be mechanically damaged the less algae kind of rear recovery capability value as resistant to mechanical damage and the recovery capability strong spirulina kind large-scale culture for spirulina.
13. the application as according to any one of claim 11 or 12, it is characterized in that, when the spirulina algae kind that the spirulina algae kind that resistant to mechanical damage ability value is bigger is less with being mechanically damaged rear recovery capability value is not same spirulina algae kind, the spirulina algae kind that resistant to mechanical damage ability value is bigger is selected to be used for the large-scale culture of spirulina.
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