CN202744558U - Photobioreactor for using haematococcus pluvialis to produce astaxanthin on large scale - Google Patents

Photobioreactor for using haematococcus pluvialis to produce astaxanthin on large scale Download PDF

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Publication number
CN202744558U
CN202744558U CN 201220408697 CN201220408697U CN202744558U CN 202744558 U CN202744558 U CN 202744558U CN 201220408697 CN201220408697 CN 201220408697 CN 201220408697 U CN201220408697 U CN 201220408697U CN 202744558 U CN202744558 U CN 202744558U
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China
Prior art keywords
pipeline group
convolution
pipeline
pipe
astaxanthin
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Withdrawn - After Issue
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CN 201220408697
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Chinese (zh)
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马建
陈黎明
黄胜奎
裴芳芳
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NINGBO HONGLONG BIO-TECHNOLOGY CO LTD
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NINGBO HONGLONG BIO-TECHNOLOGY CO LTD
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Abstract

The utility model discloses a photobioreactor for using haematococcus pluvialis to produce astaxanthin on a large scale. The photobioreactor is stable in astaxanthin yield and high in output on the condition of large-scale production. The photobioreactor comprises a liquid storage tank (1), a pipeline pump (2), a convolution pipeline set (3), a potential-of-hydrogen (pH) sensor (4), temperature sensors (5), an illumination sensor (6), a gas inlet pipe (7), an exhaust pipe (8), a conveying pipe (9), a discharge pipe (10), a culture medium pipe (11), a control unit (12), an illumination unit (13) and a spraying temperature control unit (14). The number of the convolution pipeline set (3) is at least one. A plurality of convolution pipeline sets (3) are connected in series in sequence. An inlet of the pipeline set at the head end is connected with an outlet of the pipeline pump (2). An outlet of the pipeline set at the tail end is respectively connected with the discharge pipe (10) and the liquid storage tank (1). When the photobioreactor is provided with only one convolution pipeline set (3), the pipeline set at the head end and the pipeline set at the tail end are the same pipeline set. The convolution pipeline sets (3) are arranged in a tiled mode in the same plane.

Description

Be used for the mass-producing haematococcus pulvialis and produce the bioreactor of astaxanthin
Technical field
The utility model relates to natural astaxanthin production equipment technical field, specifically is a kind of bioreactor of producing astaxanthin for the mass-producing haematococcus pulvialis.
Background technology
Haematocoocus Pluvialls (Haematococcus pluvialis) is a kind of unicell green alga, its propagation depends on cell fission, when environment is suitable, the haematococcus pulvialis biomass constantly increases, and is called " green cultivation stage ", and when being subject to the external world and coercing, such as high illumination, high temperature, the variation of pH value etc., self forms sporophyte, accumulates a large amount of astaxanthin materials and makes frustule become redness, is called " turning the red stage ".Haematococcus pulvialis receives much concern as the natural astaxanthin best source, and research finds that astaxanthin can account for the 1.5%-3.0% of dry cell weight in the haematococcus pulvialis, therefore is described as " concentrate " of natural astaxanthin.
Investigation finds that 200 tons of world today's haematococcus pulvialis annual production less thaies can not be met the need of market far away.Domestic haematococcus pulvialis industry starting is late, mostly stop laboratory research and development state, the technical matters that actual operation relates to is very complicated, so far the enterprise that grasps cultural technique and algae powder deep processing technology is very few, traced it to its cause two, one: haematococcus pulvialis cultivation yield per unit is little, is subject to microbial contamination, the algae powder yield is low, and content astaxanthin is stable not; Its two: haematococcus pulvialis " green cultivation stage " and " turning the red stage " required different condition are difficult to satisfy in the same space, be difficult for to set up low-cost, high efficiency production technology system.The Patents report of haematococcus pulvialis cultivation and production astaxanthin continues to bring out, but mostly rests on laboratory or preliminary examination stage, the domestic successful case that there is no of actual large-scale production high-content astaxanthin.
Breaking through above-mentioned haematococcus pulvialis cultivation bottleneck is current problem demanding prompt solution; the author inquires about; learn that the patent documentation that utilizes bioreactor to cultivate little algae is existing more open; be the Chinese utility model patent of CN101654653 such as notification number; but because the singularity of haematococcus pulvialis life course, described bioreactor also is not suitable for the large-scale cultivation of haematococcus pulvialis.The author has also consulted to relate to and has cultivated the patent documentation that haematococcus pulvialis is produced astaxanthin, publication number is that the Chinese utility model patent of CN1392244 then discloses a kind of method that haematococcus pulvialis is produced astaxanthin of cultivating, and what comprise culture medium prescription, one-step method production process, substratum recycles and use CO 2Regulate medium pH value and induce the formation of haematococcus pulvialis spore and the method for astaxanthin accumulation, the green cultivation stage of haematococcus pulvialis is to finish in same bio-reactor, same substratum with turning the red stage, yet does not provide the device that the mass-producing haematococcus pulvialis is produced astaxanthin; And publication number to be the Chinese utility model patent of CN1966660 disclose the large scale culturing Haematocoocus Pluvialls and transform the devices and methods therefor of astaxanthin, whole culture apparatus is comprised of photo-bioreactor system, inflation mechanism, nutrient solution infusion device and quiet cell collection device, mutually flow by nutrient solution, continuous charge is so that haematococcus pulvialis grows in division value basic synchronization; Utilize the floating dust control device and the natural water that arrange on the anchor to carry out extensive haematococcus pulvialis cultivation, make photo-bioreactor system drop to needed water depth, solved the haematococcus pulvialis cultivation and transformed these two contradictions that phase temperature, illumination and nutritional condition are different of astaxanthin; Above-mentioned three disclosed technical schemes of document have common characteristics; be that haematococcus pulvialis is cultivated and the conversion astaxanthin is finished in same device; although have its attendant advantages; but produce in the process of astaxanthin at the mass-producing haematococcus pulvialis, how so that the but always not fine solution of problem that the astaxanthin output is stablized and had high yield.
The utility model content
The technical problems to be solved in the utility model is that the bioreactor that the mass-producing haematococcus pulvialis is produced astaxanthin that is used for that high yield is stablized and had to the astaxanthin output under a kind of large-scale production condition is provided.
The technical solution of the utility model is, the utility model is used for the bioreactor that the mass-producing haematococcus pulvialis is produced astaxanthin, it comprises container for storing liquid, in-line pump, the convolution pipeline group, the pH sensor, temperature sensor, optical sensor, inlet pipe, vapor pipe, carrier pipe, discharge nozzle, cultivate parent tube, control unit, light illuminating unit, the spray control temperature unit, described container for storing liquid outlet is connected with the in-line pump entrance, described inlet pipe, vapor pipe, carrier pipe, cultivating parent tube is connected with container for storing liquid respectively, described temperature sensor, optical sensor, light illuminating unit and spray control temperature unit are electrically connected with control unit respectively, described convolution pipeline group is at least one, when being a plurality of convolution pipeline group, described a plurality of convolution pipeline group is sequentially connected, the entrance of head end pipeline group is connected with the in-line pump outlet, the outlet of terminal pipeline group respectively with discharge nozzle, container for storing liquid connects, when being a convolution pipeline group, described head end pipeline group and terminal pipeline group are same pipeline group; The described pipeline group flush system in same plane that respectively circles round distributes.
After adopting said structure, the utility model compared with prior art, have the following advantages: respectively circle round flush system distributes in pipeline group series connection and the same plane form of the utility model is not only applicable to open space and lays on a large scale, satisfy the precondition of scale operation, and each pipeline of the pipeline group of respectively circling round that flush system distributes in series connection and the same plane can be subject to illumination and the temperature effect (land return light and heat quantity of radiant energy also can take full advantage of) of equality strength under natural lighting, being beneficial to very much provides the needed illumination of haematococcus pulvialis change to red and temperature condition, and can provide evenly suitable illumination and temperature condition to obtain just the key that high yield is stablized and had to the astaxanthin output under the working condition of mass-producing, in addition, each pipeline of the pipeline group of respectively circling round that flush system distributes in series connection and the same plane is convenient to utilize temperature sensor, optical sensor, light illuminating unit, the spray control temperature unit is unified real-time monitoring, thus the impact of avoiding bad weather to cause; The utility model is used for haematococcus pulvialis and " turns the red stage ", does not comprise that haematococcus pulvialis cultivates this one-phase, and in other words, the utility model has more specific aim, thereby makes as far as possible the astaxanthin output stable and have a high yield under the working condition of mass-producing; Comprehensively above-mentioned, it is stable and have an advantage of high yield to the utlity model has astaxanthin output under the large-scale production condition.
As improvement, be connected with stopple coupon between the entrance of head end pipeline group and the in-line pump outlet, so not only sampling is convenient, is convenient to monitoring, and at this place sampling can judge more accurately that the haematococcus pulvialis in the whole bioreactor turns red degree, namely accurately judge the red results opportunity of turning.
As improvement, be provided with the U-shaped Y-junction with ball valve in each pipeline group of circling round between the adjacent channel, ball valve is closed rear ducted algae liquid can and not affect other pipelines by the Y-junction discharge, thereby is convenient to carry out pipeline cleaning and fault eliminating.
Description of drawings
Accompanying drawing is that the utility model is used for the structural representation that the mass-producing haematococcus pulvialis is produced the bioreactor of astaxanthin.
Shown in the figure, 1, container for storing liquid, 2, in-line pump, 3, convolution pipeline group, 4, the pH sensor, 5, temperature sensor, 6, optical sensor, 7, inlet pipe, 8, vapor pipe, 9, carrier pipe, 10, discharge nozzle, 11, cultivate parent tube, 12, control unit, 13, light illuminating unit, 14, the spray control temperature unit, 15, stopple coupon, 16, U-shaped Y-junction.
Embodiment
The utility model is described in further detail below in conjunction with accompanying drawing.
The utility model is used for the bioreactor that the mass-producing haematococcus pulvialis is produced astaxanthin; it comprises container for storing liquid 1; in-line pump 2; convolution pipeline group 3; pH sensor 4; temperature sensor 5; optical sensor 6; inlet pipe 7; vapor pipe 8; carrier pipe 9; discharge nozzle 10; cultivate parent tube 11; control unit 12; light illuminating unit 13; spray control temperature unit 14; described container for storing liquid 1 outlet is connected with in-line pump 2 entrances; described inlet pipe 7; vapor pipe 8; carrier pipe 9; cultivating parent tube 11 is connected with container for storing liquid 1 respectively; described temperature sensor 5; optical sensor 6; light illuminating unit 13 and spray control temperature unit 14 are electrically connected with control unit 12 respectively, and described convolution pipeline group 3 is at least one.
When being a plurality of convolution pipeline group 3, described a plurality of convolution pipeline group 3 are sequentially connected, and the entrance of head end pipeline group is connected with in-line pump 2 outlets, and the outlet of terminal pipeline group is connected with discharge nozzle 10, container for storing liquid 1 respectively.
When being a convolution pipeline group 3, described head end pipeline group and terminal pipeline group are same pipeline group.
Described pipeline group 3 flush system in same plane that respectively circles round distributes.
Be connected with stopple coupon 15 between the entrance of head end pipeline group and in-line pump 2 outlets.
Be provided with the U-shaped Y-junction 16 with ball valve in each pipeline group 3 of circling round between the adjacent channel.
In order to control break-make, inlet pipe 7, vapor pipe 8, carrier pipe 9, discharge nozzle 10, cultivate between parent tube 11, stopple coupon 15, container for storing liquid 1 and the in-line pump 2, be equipped with valve between the outlet of terminal pipeline group and the container for storing liquid 1 and control break-make, valve is prior art, does not add and gives unnecessary details.
Pipe material can be selected lucite pipe or toughened glass pipe etc.; In-line pump 2 is peristaltic pump or surge pump or vacuum primingpump etc.; Light illuminating unit 13 is led light source.
Take 10 tons of large-scale productions as example, by 2 groups of convolution pipeline group 3 series connection tiling layouts, need long 80 meters, wide 4 meters place, as shown in drawings.
Horizontal stainless steel frame structure is installed as support in ground at first on the scene, and transparent series connection parallelpiped is tiled on the support; Each pipeline group 3 top is installed among spray control temperature unit 14 and the light illuminating unit 13(figure and is processed to do to simplify, only be signal), the working order of spray control temperature unit 14 and light illuminating unit 13 is controlled by control unit 12, and control unit 12 moves by the signal that temperature sensor 5, optical sensor 6 samplings obtain; Container for storing liquid 1 and support are installed on the same level position, and container for storing liquid 1 inside is mounting temperature sensor also, and container for storing liquid 1 inside also is equipped with pH sensor 4 and thermostat, and the end of inlet pipe 7 is connected with aerator, have both played and have replenished CO 2With the effect that gas is resolved, play again the effect of stirring algae liquid; All sensor signals feed back to control unit 12, control bioreactor by control unit 12 according to setting program, thereby intelligence are adjusted to top condition, reduce labour intensity and human cost.
Be used for the bioreactor that the mass-producing haematococcus pulvialis is produced astaxanthin according to following step running the utility model:
1, reactor is disinfected: the thimerosal can that configures in container for storing liquid 1, is opened in-line pump 2 and made thimerosal be circulated in bioreactor, check simultaneously everywhere pipeline and valve.Last sterilized water repeats once, as far as possible the flush away toxic liquid according to reactor of above-mentioned steps flushing.
2, haematococcus pulvialis turns red operating process:
1 opens vapor pipe 8, the carrier pipe 9 of container for storing liquid 1 top, closes discharge nozzle 10.
2 reach 0.6 * 10 with biomass 5-1.5 * 10 5The haematococcus pulvialis liquid that the green of individual/mL is cultivated is sent in the container for storing liquid 1 by carrier pipe 9, reach certain volume after, open each sensing system of cultivating parent tube 11, inlet pipe 7 and control unit 12 controls.
3 open in-line pumps 2, and flow velocity is set as 0.2m/s-0.9m/s, beginning green algae liquid of can in the convolution pipeline group 1.
After 4 green algae liquid are filled pipeline, be back in the container for storing liquid 1.Close carrier pipe 9 this moment, and charging and discharging reach balance, thereby form closed circulation system.
5 in turning red operating process, if the ball valve of closing fault pipe end is answered in pipeline connection place leakage or pipeline breaking, this pipeline in time repaired, and it is minimum that loss is dropped to.
In turning red operating process, open stopple coupon 15 samplings, be used for the microscopically algae cell density and count, turn the index detections such as red degree.
In turning red operating process, if temperature is excessively low, by thermostat, the temperature of the algae liquid in algae liquid in the container for storing liquid and the pipeline is consistent.
In turning red operating process, if excess Temperature sprays temperature control by spray control temperature unit 14;
In turning red operating process, if pH has fluctuation, by the signal of pH sensor 4 feedbacks, the charge flow rate in control unit 12 control inlet pipe 7 carries out pH regulator.
In turning red operating process, if sticking wall situation appears in frustule, can add cleaning brush at U-shaped Y-junction 16 places, scrub by water body flow.
During the red algae of 6 results, open discharge nozzle 10, under the effect of in-line pump 2, red algae liquid extrudes pipeline group 3 through discharge nozzle 10, when red algae liquid exhausts in the container for storing liquid 1, repeating step 1 and 2, like this, then utilize power and the Water Pressure of in-line pump 2, remaining red algae liquid extrudes through discharge nozzle 10 in the pipeline group 3, and the green algae liquid of second batch has been full of pipeline group 3 simultaneously, realizes material filling and is discharged to one, greatly reduce power cost, saved the labor force.
Finished one batch in general 5-7 days, and produced continuously 3-5 batch laggard line pipe road and clean and disinfect.

Claims (3)

1. one kind is used for the bioreactor that the mass-producing haematococcus pulvialis is produced astaxanthin, it comprises container for storing liquid (1), in-line pump (2), convolution pipeline group (3), pH sensor (4), temperature sensor (5), optical sensor (6), inlet pipe (7), vapor pipe (8), carrier pipe (9), discharge nozzle (10), cultivate parent tube (11), control unit (12), light illuminating unit (13), spray control temperature unit (14), described container for storing liquid (1) outlet is connected with in-line pump (2) entrance, described inlet pipe (7), vapor pipe (8), carrier pipe (9), cultivating parent tube (11) is connected with container for storing liquid (1) respectively, described temperature sensor (5), optical sensor (6), light illuminating unit (13) and spray control temperature unit (14) are electrically connected with control unit (12) respectively, it is characterized in that, described convolution pipeline group (3) is at least one, when being a plurality of convolution pipeline group (3), described a plurality of convolution pipeline group (3) are sequentially connected, the entrance of head end pipeline group is connected with in-line pump (2) outlet, the outlet of terminal pipeline group respectively with discharge nozzle (10), container for storing liquid (1) connects, when being a convolution pipeline group (3), described head end pipeline group and terminal pipeline group are same pipeline group; Described pipeline group (3) flush system in same plane that respectively circles round distributes.
2. the bioreactor for mass-producing haematococcus pulvialis production astaxanthin according to claim 1 is characterized in that, is connected with stopple coupon (15) between the entrance of head end pipeline group and in-line pump (2) outlet.
3. the bioreactor for mass-producing haematococcus pulvialis production astaxanthin according to claim 1 is characterized in that, is provided with the U-shaped Y-junction (16) with ball valve in each pipeline group of circling round (3) between the adjacent channel.
CN 201220408697 2012-08-17 2012-08-17 Photobioreactor for using haematococcus pluvialis to produce astaxanthin on large scale Withdrawn - After Issue CN202744558U (en)

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102827766A (en) * 2012-08-17 2012-12-19 宁波红龙生物科技有限公司 Photobioreactor for large-scale production of astaxanthin by haematococcus pluvialis
CN104762212A (en) * 2015-04-16 2015-07-08 青岛华盛绿能农业科技有限公司 Method for culturing haematococcus pluvialis by photovoltaic greenhouse

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102827766A (en) * 2012-08-17 2012-12-19 宁波红龙生物科技有限公司 Photobioreactor for large-scale production of astaxanthin by haematococcus pluvialis
CN104762212A (en) * 2015-04-16 2015-07-08 青岛华盛绿能农业科技有限公司 Method for culturing haematococcus pluvialis by photovoltaic greenhouse

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Granted publication date: 20130220

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