CN201301313Y - Photosynthetic bacteria culturing device - Google Patents

Photosynthetic bacteria culturing device Download PDF

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Publication number
CN201301313Y
CN201301313Y CN 200820169441 CN200820169441U CN201301313Y CN 201301313 Y CN201301313 Y CN 201301313Y CN 200820169441 CN200820169441 CN 200820169441 CN 200820169441 U CN200820169441 U CN 200820169441U CN 201301313 Y CN201301313 Y CN 201301313Y
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CN
China
Prior art keywords
irradiation source
photosynthetic bacterium
water pump
column
light source
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Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CN 200820169441
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Chinese (zh)
Inventor
张德民
潘志崇
陈和平
章文军
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Ningbo University
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Ningbo University
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Publication date
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Priority to CN 200820169441 priority Critical patent/CN201301313Y/en
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Publication of CN201301313Y publication Critical patent/CN201301313Y/en
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00—Bioreactors or fermenters specially adapted for specific uses
    • C12M21/02—Photobioreactors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/20—Material Coatings
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M31/00—Means for providing, directing, scattering or concentrating light
    • C12M31/02—Means for providing, directing, scattering or concentrating light located outside the reactor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M31/00—Means for providing, directing, scattering or concentrating light
    • C12M31/10—Means for providing, directing, scattering or concentrating light by light emitting elements located inside the reactor, e.g. LED or OLED

Abstract

The utility model relates to a photosynthetic bacteria culturing device, which comprises a transmission column-shaped culture container, an internal radiation light source, an outer radiation light source, an non-transmission outer bucket, a container cover, a water pump, an upper cycling pipeline and a lower cycling pipeline, wherein the column-shaped culture container is a hollow column body, and the internal radiation light source is arranged inside the hollow space of the column-shaped culture container, and the inner wall of the outer bucket is made of reflection material, and the outer radiation light source is uniformly arranged on the inner wall of the outer bucket, and the culture container is connected with the water pump through the upper and the lower cycling pipelines to form a closed cycling. The photosynthetic bacteria culturing device has the advantages that the light receiving area of the bacteria liquid is greatly increased, and the utilization rate of the light energy is effectively improved, and the production efficiency and the product quality are improved. With the disassemble outer bucket, the photosynthetic bacteria culturing device not only can be cultured by the outdoor natural light, but also can be provided with the outer radiation light source to be cultured indoor. The structure is simple and the installation operation is very convenient. The discharging way can be free to choose according to the real requirements, thereby improving the automation degree of the feeding and discharging, and being applicable to the mass production.

Description

A kind of photosynthetic bacterium culture apparatus
Technical field
The utility model relates to the culture apparatus of photosynthetic bacterium, particularly a kind ofly utilizes double-layer illumination fast and the device of large-scale production photosynthetic bacteria preparation.
Background technology
Photosynthetic bacterium is a kind of photosynthetic anaerobic prokaryotic organism, has a wide range of applications in fields such as aquaculture, poultry industry, purification of water quality and new energy developments.
Photosynthetic bacterium is mainly cultivated under the illumination anaerobic condition and produces, and illumination condition is one of important factor of decision photosynthetic bacterium growth speed, and the quality of lighting effect directly has influence on the production efficiency and the quality of production of photosynthetic bacterium.The illumination source of present photosynthetic bacterium culture apparatus is single external container or the inside of being arranged on usually, and be pointolite, this culture apparatus exists light loss rate height, bacterium liquid to be subjected to shortcomings such as light is inhomogeneous, local temperature is higher, thalli growth is slow, production efficiency is lower.
Photosynthetic bacterium has the adherent phenomenon of the light of becoming usually in culturing process, often stir nutrient solution with the transparence that keeps container, make bacterium liquid be subjected to light even.That present culture apparatus normally leaves standstill or regular the former can not solve the adherent phenomenon of thalline and the problem that bacterium liquid is subjected to the light inequality, has reduced the utilization ratio of light, thereby has influenced production efficiency and quality product with manually stirring, and latter's labour intensity is big.In addition, present culture apparatus usually can not continuous production and level of automation not high, manually finish as the interpolation of culture medium raw material is all handy, be not suitable for large-scale production.
The utility model content
Technical problem to be solved in the utility model is to overcome the above-mentioned deficiency of prior art and a kind of photosynthetic bacterium culture apparatus is provided; improved effectively luminous energy utilization, solved the adherent phenomenon of thalline and bacterium liquid is subjected to the unequal problem of light; production efficiency and quality product all are significantly improved, are fit to the quick and large-scale production of photosynthetic bacterium.
The utility model solves the problems of the technologies described above the technical scheme that is adopted:
A kind of photosynthetic bacterium culture apparatus, it is characterized in that: comprise the column culture vessel of printing opacity, interior irradiation source, outer irradiation source, lighttight overcoat bucket, container cover, water pump and upper and lower circulating line, described column culture vessel is the cylinder of hollow, irradiation source in place in middle vacancy, described overcoat bucket inwall is a light reflecting material, outer irradiation source evenly is installed on the overcoat bucket inwall, and culture vessel links to each other with water pump by cocycle pipeline, following circulating line and constitutes closed cycle.
Above-mentioned overcoat bucket removably is fixed on the column culture vessel.
Above-mentioned column culture vessel also can be cylindric or other shapes.
Compared with prior art, advantage of the present utility model is:
1. the employing double-layer illumination increases bacterium liquid light-receiving area greatly; Overcoat bucket inwall is a light reflecting material, has improved the utilization ratio of luminous energy effectively.
2. utilize water pump circulation nutrient solution, solved the adherent phenomenon of thalline effectively, make thalline be subjected to light more even simultaneously, thereby accelerated the growth and breeding of photosynthetic bacterium, improved production efficiency.Water pump also has the effect that pump advances culture medium raw material and pumps the nutrient solution finished product concurrently.
3. adopt the hermetic type culture vessel, completely cut off oxygen effectively, suppressed the growth of assorted bacterium, improved quality product.
4. by four combinations that opening-closing valve is different, can freely select discharge method according to actual needs, improve the level of automation of input and output material, be fit to large-scale production.
5. control the temperature variation of nutrient solution in the culturing process in real time by temperature controller.
6. the overcoat bucket is detachable, both can utilize natural light in outdoor cultivation, can load onto outer irradiation source again and be placed on indoor cultivation.Whole apparatus structure is simple, and fitting operation is very convenient.
Description of drawings
Fig. 1 is the structural representation of facing of the utility model embodiment.
Fig. 2 is the plan structure synoptic diagram of the utility model embodiment.
Fig. 3 is the perspective view of the utility model embodiment.
Embodiment
Embodiment describes in further detail the utility model below in conjunction with accompanying drawing.
As Fig. 1, Fig. 2, shown in Figure 3, the cylindric culture vessel 1 of hollow, adopting thickness is bonding the forming of synthetic glass of 8mm, the fluorescent tube 2 in irradiation source in place in middle vacancy, overcoat bucket 3 adopts light-proof material to make, and overcoat bucket 3 inwalls are light reflecting material 4, and the fluorescent tube 5 in outer irradiation source evenly is installed on overcoat bucket 3 inwalls, thereby the formation double-layer illumination can be regulated the quantity of outer irradiation source fluorescent tube in use as required to control suitable intensity of illumination simultaneously.
Above-mentioned overcoat bucket 3 is detachable to be fixed on the cylindric culture vessel 1 of hollow, can be for convenience detach, and when outdoor, utilize natural lighting to cultivate.
Culture vessel 1 top is one to stud with the circular container cover 6 of sealing-ring, and lid 6 is provided with temperature controller 7 and spillway 8.The bottom of culture vessel 1 has nutrient solution discharge port 9, be connected with following circulation tube 10, following circulation tube 10 links to each other by water pump 14 with upper circular tube 18, respectively connect a drain pipe 21,22 on following circulation tube 10 and the upper circular tube 18, respectively be with valve 11, valve 12, valve 17, valve 15 on following circulation tube 10, drain pipe 21, upper circular tube 18, the drain pipe 22, there are four opening- closing valves 11,12,15,17 water pump 14 front and back like this, as shown in Figure 1, the mouth of pipe 13 is the charging and discharging mouth, and the mouth of pipe 16 is a discharge port.When valve 11 and 12 is opened, and valve 15 and 17 can directly utilize liquid pressure to discharge nutrient solution when closing; When valve 11 and 15 is opened, when valve 12 and 17 is closed, can utilize water pump that liquid is pumped fast; And in the operation culturing process, valve 11 and 17 is opened, valve 12 and 15 is closed, and utilizes the water pump can be to the liquid in container stirring that circulates; When valve-off 11 and 15, can utilize water pump to advance culture medium raw material when opening valve 12 and 17 from 13 pumps.
Above-mentioned column culture vessel 1, interior irradiation source 2, outer irradiation source 5, overcoat bucket 3, water pump 14 and cocycle pipeline 18, down circulating line 10 places on the support 19 that forms with angle bar weldering system, sees Fig. 3.

Claims (10)

1, a kind of photosynthetic bacterium culture apparatus, it is characterized in that: the column culture vessel (1) that comprises printing opacity, interior irradiation source (2), outer irradiation source (5), lighttight overcoat bucket (3), container cover (6), water pump (14), cocycle pipeline (18), following circulating line (10), described column culture vessel (1) is the right cylinder of hollow, irradiation source (2) in place in middle vacancy, described overcoat bucket (3) inwall is light reflecting material (4), outer irradiation source (5) evenly is installed on overcoat bucket (3) inwall, and culture vessel (1) is by cocycle pipeline (18), following circulating line (10) links to each other with water pump (14) and constitutes closed cycle.
2, photosynthetic bacterium culture apparatus according to claim 1 is characterized in that: overcoat bucket (3) removably is fixed on the column culture vessel (1).
3, photosynthetic bacterium culture apparatus according to claim 1 and 2, it is characterized in that: respectively connect a drain pipe (21), (22) on described cocycle pipeline (18), the following circulating line (10), all be with valve (11), (12), (15) and (17) on following circulation tube (10), upper circular tube (18), drain pipe (21), the drain pipe (22).
4, according to claim 1 or 2 described photosynthetic bacterium culture apparatuses, it is characterized in that: described container cover (6) is provided with temperature controller (7) and spillway (8).
5, according to the described photosynthetic bacterium culture apparatus of claim 3, it is characterized in that: described container cover (6) is provided with temperature controller (7) and spillway (8).
6, according to claim 1 or 2 described photosynthetic bacterium culture apparatuses, it is characterized in that: described column culture vessel (1), interior irradiation source (2), outer irradiation source (5), overcoat bucket (3), water pump (14) and cocycle pipeline (18), following circulating line (10) place on the support (19).
7, according to the described photosynthetic bacterium culture apparatus of claim 3, it is characterized in that: described column culture vessel (1), interior irradiation source (2), outer irradiation source (5), overcoat bucket (3), water pump (14) and cocycle pipeline (18), following circulating line (10) place on the support (19).
8, according to the described photosynthetic bacterium culture apparatus of claim 5, it is characterized in that: described column culture vessel (1), interior irradiation source (2), outer irradiation source (5), overcoat bucket (3), water pump (14) and cocycle pipeline (18), following circulating line (10) place on the support (19).
9, according to claim 1 or 2 described photosynthetic bacterium culture apparatuses, it is characterized in that: irradiation source (2) and outer irradiation source (5) are fluorescent tube in described.
10, according to the described photosynthetic bacterium culture apparatus of claim 8, it is characterized in that: irradiation source (2) and outer irradiation source (5) are fluorescent tube in described.
CN 200820169441 2008-11-29 2008-11-29 Photosynthetic bacteria culturing device Expired - Fee Related CN201301313Y (en)

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Application Number Priority Date Filing Date Title
CN 200820169441 CN201301313Y (en) 2008-11-29 2008-11-29 Photosynthetic bacteria culturing device

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Application Number Priority Date Filing Date Title
CN 200820169441 CN201301313Y (en) 2008-11-29 2008-11-29 Photosynthetic bacteria culturing device

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102021113A (en) * 2010-09-21 2011-04-20 钱正浩 Portable anaerobic microorganism culturing device
CN106010939A (en) * 2016-05-23 2016-10-12 嘉兴职业技术学院 Alga culture device
CN114525197A (en) * 2022-04-22 2022-05-24 深圳市路阳农业科技有限公司 Photosynthetic microorganism culture apparatus

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102021113A (en) * 2010-09-21 2011-04-20 钱正浩 Portable anaerobic microorganism culturing device
CN106010939A (en) * 2016-05-23 2016-10-12 嘉兴职业技术学院 Alga culture device
CN106010939B (en) * 2016-05-23 2018-04-06 嘉兴职业技术学院 A kind of algatron
CN114525197A (en) * 2022-04-22 2022-05-24 深圳市路阳农业科技有限公司 Photosynthetic microorganism culture apparatus

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Granted publication date: 20090902

Termination date: 20111129