CN100453635C - Device and method for large scale culturing Haemotococcum pluvies and converting astaxanthin - Google Patents
Device and method for large scale culturing Haemotococcum pluvies and converting astaxanthin Download PDFInfo
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- CN100453635C CN100453635C CNB2006101546780A CN200610154678A CN100453635C CN 100453635 C CN100453635 C CN 100453635C CN B2006101546780 A CNB2006101546780 A CN B2006101546780A CN 200610154678 A CN200610154678 A CN 200610154678A CN 100453635 C CN100453635 C CN 100453635C
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- JEBFVOLFMLUKLF-IFPLVEIFSA-N Astaxanthin Natural products CC(=C/C=C/C(=C/C=C/C1=C(C)C(=O)C(O)CC1(C)C)/C)C=CC=C(/C)C=CC=C(/C)C=CC2=C(C)C(=O)C(O)CC2(C)C JEBFVOLFMLUKLF-IFPLVEIFSA-N 0.000 title claims abstract description 49
- 235000013793 astaxanthin Nutrition 0.000 title claims abstract description 49
- MQZIGYBFDRPAKN-ZWAPEEGVSA-N astaxanthin Chemical compound C([C@H](O)C(=O)C=1C)C(C)(C)C=1/C=C/C(/C)=C/C=C/C(/C)=C/C=C/C=C(C)C=CC=C(C)C=CC1=C(C)C(=O)[C@@H](O)CC1(C)C MQZIGYBFDRPAKN-ZWAPEEGVSA-N 0.000 title claims abstract description 49
- 229940022405 astaxanthin Drugs 0.000 title claims abstract description 49
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/02—Photobioreactors
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/06—Tubular
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/56—Floating elements
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/50—Improvements relating to the production of bulk chemicals
- Y02P20/582—Recycling of unreacted starting or intermediate materials
Abstract
The invention discloses a set of apparatus and a method for culturing Haematococcus pluvialis and converting astaxanthin in large scale. The apparatus consists of a photo-bioreactor system set on the frisket, aeration equipment, culture fluid inoculation equipment and still cell harvesting equipment. Floating or sunken pulse equipment is set on the frisket. The apparatus don't need establishment of artificial culture pool and other institution, but uses natural water body to culture Haematococcus pluvialis in large scale. It is low cost, the culture temperature, illumination and water flow rate is stable, culture fluid flows with each other, aeration is continuous, culture conditions are precedent, which make the growth and increment of Haematococcus pluvialis almost at equal pace. The growth rate of Haematococcus pluvialis can reach to 25%, biomass increase is fast, doubles 3-5 days. The photo-bioreactor can be hoisted into required depth of the water, which solves the contradiction of difference of temperature, illumination and nutrition of Haematococcus pluvialis culture and astaxanthin conversion.
Description
Technical field
The present invention relates to a kind of culture apparatus of Haematocoocus Pluvialls, especially relate to the devices and methods therefor of a kind of large scale culturing Haematocoocus Pluvialls and conversion astaxanthin.
Background technology
Haematocoocus Pluvialls (Haema tococcus pluvialis) is a kind of unicell green alga, and its propagation depends on cell fission, and this algae can accumulate astaxanthin (Astaxanthin) in a large number in the specified phase of growth.Astaxanthin has good tinctorial property and extremely strong antioxidant property, is mainly used at present in the aquaculture, can be used as the feed coloring agent and the additive of salmon trout class, to improve the growth and the physiological function of salmon trout class; Also be the good additive of human food prods and healthcare products simultaneously, can improve immunizing power, suppress cancer.Therefore, the application aspect medical also has very big potentiality.Hence one can see that, and Haematocoocus Pluvialls is the economic little algae with good exploitation future.
The Haematocoocus Pluvialls vast market prospect has caused common concern both domestic and external, and the technology of the natural astaxanthin of overseas utilization Haematocoocus Pluvialls production has at present obtained tremendous development, and the existing company of Sweden adopts source of artificial light irradiating biological reactor to produce.U.S. Aquasearch company cultivates in computer-controlled outdoor sealing bio-reactor, and Cyanotech company then adopts " platform " formula bio-reactor of sealing to cultivate.At present domestic have many universities and colleges and scientific research institutions to study cultivating and extract aspects such as natural astaxanthin, many achievements in research have been obtained, a kind of airlift bioreactor of Haematocoocus Pluvialls high-density culture was disclosed in the disclosed No. 200510018203.4 Chinese invention patent ublic specification of application as on October 12nd, 2005, comprise bioreactor, water treatment device, illuminating apparatus and gas supply device, it is characterized in that water treatment device is positioned at the bio-reactor outside, illuminating apparatus is positioned at the bio-reactor side, illuminating apparatus comprises built-in source and external source, temperature-control device is positioned at the bio-reactor bottom, it on temperature-control device gas supply device, the sampling venting hole is positioned at the reactor head inboard, and algae liquid is collected mouth and is positioned at the bio-reactor bottom.On December 22nd, 2004, disclosed CN02138827.X number Chinese invention patent then disclosed a kind of method of cultivating haematococcus pluvialis to produce astaxanthin, comprise culture medium prescription, one-step method production process, the recycling and regulate medium pH value with carbonic acid gas and induce the formation of haematococcus pulvialis spore and the method for astaxanthin accumulation of substratum, the growth of the vegetative cell of Haematocoocus Pluvialls, spore transforms and the astaxanthin accumulation is at same bio-reactor, finish in the same substratum, pH value by the regulation and control nutrient solution promotes spore to transform and the astaxanthin accumulation, culture cycle is 12～15 days, substratum can recycle 6 times through recovery-processing-preparation again at least.
But these achievement in research majorities still are in small test in the phase at present, in the pond of manually building up, carry out open cultivation, by other swim animals and plants or Institute of Micro-biology's pollution, can not form scale operation easily, therefore and between the commercialization production also have a segment distance.In actual production, though cultivate Haematocoocus Pluvialls by the bio-reactor or the airlift bioreactor that adopt duct type bio-reactor or small stationary system to form at present, the defective of its existence is under fairly large working condition, increase the equipment input of a large amount of controlled temperature, production cost will increase greatly.On unit space utilizes, increase the pipeline of duct type bio-reactor, need greatly to increase the cost of building, add that the frustule adhesion covers, cause that light transmission descends between incubation period, cause that duct wall cleans difficulty, small-scale production can only suit.
At present, Shang Weiyou utilizes natural water, especially utilize the nature water body to carry out the large scale culturing Haematocoocus Pluvialls and obtain the production method of astaxanthin, wherein chief reason is that the Haematocoocus Pluvialls cultivation period needs normal temperature, the low light level, high nutritive salt, transforms the generation astaxanthin and then need carry out under the condition of high temperature, high light, low nutritive salt.Be that the Haematocoocus Pluvialls cultivation transforms with Haematocoocus Pluvialls and the accumulation astaxanthin is to be in two different stages, need different envrionment conditionss.Therefore, need adopt different bio-reactors and different nutrient solutions respectively usually in these two stages, carry out under different envrionment conditionss, this method is referred to as " two-step approach " usually and produces.
Summary of the invention
Technical problem to be solved by this invention provides a kind of large scale culturing Haematocoocus Pluvialls and the devices and methods therefor that transforms astaxanthin, can solve and cultivate in the culturing process and the contradiction that transforms required temperature and illumination, both ensured the Haematocoocus Pluvialls ramp, produce enough biomasss, can guarantee that again the Haematocoocus Pluvialls of having cultivated can transform and accumulate astaxanthin rapidly.
The present invention solves the problems of the technologies described above the technical scheme that is adopted: the device of a kind of large scale culturing Haematocoocus Pluvialls and conversion astaxanthin, it comprises anchor and the photo-bioreactor system that is arranged on the described anchor, inflation mechanism, nutrient solution infusion device and quiet cell collection device, described photo-bioreactor system is cascadedly combined by the unit light bio-reactor that the waterproof polythene material or the inflexible synthetic glass of two flexibilities constitutes at least, described inflation mechanism is connected with the front end of top first module bioreactor in the described photo-bioreactor system by flexible pipe with described nutrient solution infusion device, described quiet cell collection device is connected by the end of the second unit light bio-reactor in flexible pipe and the described photo-bioreactor system, the described second unit light bio-reactor is meant the unit light bio-reactor of last position in the described photo-bioreactor system, and described anchor is provided with the control device that drifts along.
The described control device that drifts along is made up of the float and the weight that are arranged on the anchor.
Can be provided with emergency switch between the described unit light bio-reactor.
Described unit light bio-reactor can be made by hard or flexible materials, can be connected in series mutually by flexible pipe between each unit light bio-reactor.
Can be provided with the nutrient solution circulating line between described first module bioreactor and the described second unit light bio-reactor.
Described inflation mechanism is made up of air pump and acidic liquid container, described air pump is connected with described acidic liquid container by flexible pipe, described nutrient solution infusion device is made up of first water pump and wort cylinder, described first water pump is connected with described wort cylinder by flexible pipe, and described first water pump is connected with described first module bioreactor respectively by flexible pipe with described acidic liquid container.
Described quiet cell collection device comprises second water pump and cultured products holding tank, the import of described second water pump is connected by the end of flexible pipe with the described second unit light bio-reactor, and the outlet of described second water pump is connected with described cultured products holding tank by flexible pipe.
Use the method for above-mentioned device large scale culturing Haematocoocus Pluvialls and conversion astaxanthin, it may further comprise the steps: 1. inculcate nutrient solution by described nutrient solution infusion device in described photo-bioreactor system, wherein the nitrogen content in the nutrient solution is that 5～20mg/L, phosphorus content are 0.5～2.0mg/L; 2. inoculation is 3. by the continuous charge in described photo-bioreactor system of described inflation mechanism, and increase gas mixes with nutrient solution; 4. by regulating the described control device that drifts along whole culture apparatus is arranged in the water field water depth of suitable haematococcus pluvialis growing, the water temperature of environment is 15 ~ 25 ℃, and the intensity of illumination of environment is 1000～5000Lx; 5. after cultivating 15 days, when the density of frustule reaches 10～300,000/milliliter, by regulating the described control device that drifts along whole culture apparatus is promoted to the water depth that the Haematocoocus Pluvialls that suits in the water field transformed and accumulated astaxanthin, the water temperature of environment is 25～30 ℃, and the intensity of illumination of environment is 6000～25000Lx; 6. by the continuous charge in described photo-bioreactor system of described inflation mechanism; The quiet cell harvesting that 7. will be deposited on described second unit light bio-reactor bottom carries out drying then in described quiet cell collection device, obtain a large amount of quiet cells that is rich in astaxanthin.
The described control device that drifts along is made up of the float and the weight that are arranged on the anchor, and the water depth of culture apparatus setting is realized by regulating described float or described weight.
Described quiet cell collection device comprises second water pump and cultured products holding tank, the import of described second water pump is connected by the end of flexible pipe with the described second unit light bio-reactor, the outlet of described second water pump is connected with described cultured products holding tank by flexible pipe, and described second water pump will be deposited on the quiet cell harvesting of described second unit light bio-reactor bottom in described cultured products holding tank.
Compared with prior art, the invention has the advantages that whole culture apparatus is by the photo-bioreactor system that is arranged on the anchor, inflation mechanism, nutrient solution infusion device and quiet cell collection device are formed, photo-bioreactor system is cascadedly combined by at least two unit light bio-reactors, inflation mechanism is connected with the front end of top first module bioreactor in the photo-bioreactor system by flexible pipe with the nutrient solution infusion device, quiet cell collection device is connected with the rearmost second unit light bio-reactor in the photo-bioreactor system is terminal by flexible pipe, anchor is provided with the control device that drifts along, can not need set up artificial culture pond and other facilities, cultivate and utilize natural water to carry out extensive Haematocoocus Pluvialls, reduced investment, save cost, improved economic return; Cultivate owing to carry out Haematocoocus Pluvialls in the big water body of nature, water body environment is stable, and the water body scope is big, and the water body exchange is fast, provides environment-guarantee for cultivating high-quality Haematocoocus Pluvialls and obtaining high-quality astaxanthin; Utilizing natural water body to carry out extensive Haematocoocus Pluvialls cultivates, because envrionment conditionss such as the temperature of its cultivation, illumination, flow velocity are more stable, link to each other between the unit bio-reactor, nutrient solution flows mutually, continuous charge in the bio-reactor in addition, culture condition is superior, make the growth and the division growth basic synchronization of Haematocoocus Pluvialls, the cultivation of suitable trophic level in addition, the growth rate of Haematocoocus Pluvialls reaches 25%, biomass increases fast, can double in general 3～5 days; And the control device that drifts along by being provided with on the anchor, can be in same water body photo-bioreactor system be elevated to needed water depth, solved preferably that Haematocoocus Pluvialls is cultivated and transformed these two stages of astaxanthin and needed differing temps, different light and Different Nutrition condition this to contradiction, in same photo-bioreactor system, just can cultivate continuously and transform with same nutrient solution, the two step method in past is merged into single stage method, simplified production process, alleviated labour intensity, its photo-bioreactor system can use continuously, has saved resource; Use apparatus and method of the present invention, the growth of Haematocoocus Pluvialls and division growth basic synchronization, making culturing cell be converted into the same stage of quiet cell finishes, the transformation efficiency that Haematocoocus Pluvialls transforms astaxanthin can reach more than 90%, the content of astaxanthin can reach 1.3%～2.1%, between each unit light bio-reactor, emergency switch is set, can be under special circumstances (when leaking) by closing emergency switch, continue to leak with the unit light bio-reactor of avoiding other.
Description of drawings
Fig. 1 is the structural representation of cultivation of the present invention and conversion system.
Embodiment
Embodiment describes in further detail the present invention below in conjunction with accompanying drawing.
Embodiment one: the device of a kind of large scale culturing Haematocoocus Pluvialls and conversion astaxanthin, it comprises anchor (figure show) and is arranged on photo-bioreactor system 1 on the anchor, inflation mechanism 2, nutrient solution infusion device 3 and quiet cell collection device 4, photo-bioreactor system 1 is cascadedly combined by five unit light bio-reactors, the unit light bio-reactor is made by the waterproof polythene material of flexibility, be connected in series mutually by flexible pipe between each unit light bio-reactor, flexible pipe between the unit light bio-reactor is provided with emergency switch 5, inflation mechanism 2 is made up of air pump 21 and acidic liquid container 22, air pump 21 is connected with acidic liquid container 22 by flexible pipe, nutrient solution infusion device 3 is made up of first water pump 31 and wort cylinder 32, first water pump 31 is connected with wort cylinder 32 by flexible pipe, first water pump 31 is connected with first module bioreactor 11 respectively by flexible pipe with acidic liquid container 22, the pressurized gas that air pump 21 is produced enters in the acidic liquid container 22 by flexible pipe, acidic liquid container 22 is provided with the net filter that do not absorb water, the bubble that high pressure gas produced is stopped, gas behind peracid Xian enters in the photo-bioreactor system 1 by a flexible pipe and mixes with nutrient solution, quiet cell collection device 4 comprises second water pump 41 and cultured products holding tank 42, the import of second water pump 41 is connected by the bottom of flexible pipe with the second unit light bio-reactor 12, the outlet of second water pump 41 is connected with cultured products holding tank 42 by flexible pipe, and anchor is provided with by float and weight forms the control device that drifts along (figure does not show).
Embodiment two: other structure is identical with embodiment one, and difference is that the unit light bio-reactor made by the inflexible synthetic glass.
Embodiment three: other structure is identical with embodiment one, difference is that photo-bioreactor system 1 is made up of ten unit light bio-reactors, the unit light bio-reactor is made by the flexible polyethylene material, be provided with nutrient solution circulating line (figure does not show) between the first module bioreactor 11 and the second unit light bio-reactor 12, nutrient solution circulates in photo-bioreactor system 1, can improve the utilization ratio of inflation.
Embodiment four: use the device of the foregoing description to carry out large scale culturing Haematocoocus Pluvialls and the method that transforms astaxanthin, it may further comprise the steps: 1. inculcate nutrient solution by nutrient solution infusion device 3 in photo-bioreactor system 1, wherein the nitrogen content in the nutrient solution is that 10mg/L, phosphorus content are 0.8mg/L; 2. inoculation; 3. by inflation mechanism 2 continuous charge in photo-bioreactor system 1, increase gas mixes with nutrient solution; 4. be arranged on float and weight on the anchor by adjusting, whole culture apparatus is arranged on the water depth of suitable haematococcus pluvialis growing in the water field, the water temperature of environment is 20 ℃, and the intensity of illumination of environment is 3000Lx; 5. after cultivating 15 days, when the density of frustule reaches 200,000/milliliter, be arranged on float and weight on the anchor by adjusting, whole culture apparatus is promoted to the water depth that the Haematocoocus Pluvialls that suits in the water field transformed and accumulated astaxanthin, the water temperature of environment is 28 ℃, and the intensity of illumination of environment is 20000Lx; 6. by inflation mechanism 2 continuous charge in photo-bioreactor system 1; 7. the quiet cell harvesting that will be deposited on the second unit light bio-reactor, 22 bottoms by second water pump 41 carries out drying then in cultured products holding tank 42, obtains astaxanthin.
Embodiment five: use the device of the foregoing description to carry out large scale culturing Haematocoocus Pluvialls and the method that transforms astaxanthin, it may further comprise the steps: 1. inculcate nutrient solution by nutrient solution infusion device 3 in photo-bioreactor system 1, wherein the nitrogen content in the nutrient solution is that 5mg/L, phosphorus content are 1.0mg/L; 2. inoculation; 3. by inflation mechanism 2 continuous charge in photo-bioreactor system 1, increase gas mixes with nutrient solution; 4. be arranged on float and weight on the anchor by adjusting, whole culture apparatus is arranged on the water depth of suitable haematococcus pluvialis growing in the water field, the water temperature of environment is 15 ℃, and the intensity of illumination of environment is 1000Lx; 5. after cultivating 15 days, when the density of frustule reaches 100,000/milliliter, be arranged on float and weight on the anchor by adjusting, whole culture apparatus is promoted to the water depth that the Haematocoocus Pluvialls that suits in the water field transformed and accumulated astaxanthin, the water temperature of environment is 25 ℃, and the intensity of illumination of environment is 6000Lx; 6. by inflation mechanism 2 continuous charge in photo-bioreactor system 1; 7. the quiet cell harvesting that will be deposited on the second unit light bio-reactor, 22 bottoms by second water pump 41 carries out drying then in cultured products holding tank 42, obtains astaxanthin.
Embodiment six: use the device of the foregoing description to carry out large scale culturing Haematocoocus Pluvialls and the method that transforms astaxanthin, it may further comprise the steps: 1. inculcate nutrient solution by nutrient solution infusion device 3 in photo-bioreactor system 1, wherein the nitrogen content in the nutrient solution is that 8mg/L, phosphorus content are 0.5mg/L; 2. inoculation; 3. by inflation mechanism 2 continuous charge in photo-bioreactor system 1, increase gas mixes with nutrient solution; 4. be arranged on float and weight on the anchor by adjusting, whole culture apparatus is arranged on the water depth of suitable haematococcus pluvialis growing in the water field, the water temperature of environment is 25 ℃, and the intensity of illumination of environment is 5000Lx; 5. after cultivating 15 days, when the density of frustule reaches 300,000/milliliter, be arranged on float and weight on the anchor by adjusting, whole culture apparatus is promoted to the water depth that the Haematocoocus Pluvialls that suits in the water field transformed and accumulated astaxanthin, the water temperature of environment is 30 ℃, and the intensity of illumination of environment is 25000Lx; 6. by inflation mechanism 2 continuous charge in photo-bioreactor system 1; 7. the quiet cell harvesting that will be deposited on the second unit light bio-reactor, 22 bottoms by second water pump 41 carries out drying then in cultured products holding tank 42, obtains astaxanthin.
In embodiment four, five, six, after finishing all culturing process, can inculcate clear water or thimerosal to photo-bioreactor system 1 with nutrient solution infusion device 3, the air-flow and the liquid stream that utilize inflation mechanism 2 to produce clean photo-bioreactor system 1, and with quiet cell collection device 4 clear water or thimerosal are drained, make whole culture apparatus enter repeated use.
Claims (10)
1, the device of a kind of large scale culturing Haematocoocus Pluvialls and conversion astaxanthin, it is characterized in that it comprises anchor and the photo-bioreactor system that is arranged on the described anchor, inflation mechanism, nutrient solution infusion device and quiet cell collection device, described photo-bioreactor system is cascadedly combined by the unit light bio-reactor that the waterproof polythene material or the inflexible synthetic glass of two flexibilities constitutes at least, described inflation mechanism is connected with the front end of top first module bioreactor in the described photo-bioreactor system by flexible pipe with described nutrient solution infusion device, described quiet cell collection device is connected by the end of the second unit light bio-reactor in flexible pipe and the described photo-bioreactor system, the described second unit light bio-reactor is meant the unit light bio-reactor of last position in the described photo-bioreactor system, and described anchor is provided with the control device that drifts along.
2, large scale culturing Haematocoocus Pluvialls as claimed in claim 1 and transform the device of astaxanthin is characterized in that the described control device that drifts along is made up of the float and the weight that are arranged on the anchor.
3, the device of large scale culturing Haematocoocus Pluvialls as claimed in claim 1 and conversion astaxanthin is characterized in that being provided with emergency switch between the described unit light bio-reactor.
4, large scale culturing Haematocoocus Pluvialls as claimed in claim 1 and transform the device of astaxanthin is characterized in that described unit light bio-reactor made by hard or flexible materials, is connected in series mutually by flexible pipe between each unit light bio-reactor.
5, the device of large scale culturing Haematocoocus Pluvialls as claimed in claim 1 and conversion astaxanthin is characterized in that being provided with the nutrient solution circulating line between described first module bioreactor and the described second unit light bio-reactor.
6, the device of large scale culturing Haematocoocus Pluvialls as claimed in claim 1 and conversion astaxanthin, it is characterized in that described inflation mechanism is made up of air pump and acidic liquid container, described air pump is connected with described acidic liquid container by flexible pipe, described nutrient solution infusion device is made up of first water pump and wort cylinder, described first water pump is connected with described wort cylinder by flexible pipe, and described first water pump is connected with described first module bioreactor respectively by flexible pipe with described acidic liquid container.
7, the device of large scale culturing Haematocoocus Pluvialls as claimed in claim 1 and conversion astaxanthin, it is characterized in that described quiet cell collection device comprises second water pump and cultured products holding tank, the import of described second water pump is connected by the end of flexible pipe with the described second unit light bio-reactor, and the outlet of described second water pump is connected with described cultured products holding tank by flexible pipe.
8, use the method for claim 1 described device large scale culturing Haematocoocus Pluvialls and conversion astaxanthin, it is characterized in that it may further comprise the steps: 1. inculcate nutrient solution by described nutrient solution infusion device in described photo-bioreactor system, wherein the nitrogen content in the nutrient solution is that 5～10mg/L, phosphorus content are 0.5～1.0mg/L; 2. inoculation; 3. by the continuous charge in described photo-bioreactor system of described inflation mechanism, increase gas mixes with nutrient solution; 4. by regulating the described control device that drifts along whole culture apparatus is arranged in the water field water depth of suitable haematococcus pluvialis growing, the water temperature of environment is 15～25 ℃, and the intensity of illumination of environment is 1000～5000Lx; 5. after cultivating 15 days, when the density of frustule reaches 10～300,000/milliliter, by regulating the described control device that drifts along whole culture apparatus is promoted to the water depth that the Haematocoocus Pluvialls that suits in the water field transformed and accumulated astaxanthin, the water temperature of environment is 25～30 ℃, and the intensity of illumination of environment is 6000～25000Lx; 6. by the continuous charge in described photo-bioreactor system of described inflation mechanism; The quiet cell harvesting that 7. will be deposited on described second unit light bio-reactor bottom carries out drying then in described quiet cell collection device, obtain astaxanthin.
9, the method for large scale culturing Haematocoocus Pluvialls as claimed in claim 8 and conversion astaxanthin, it is characterized in that the described control device that drifts along is made up of the float and the weight that are arranged on the anchor, the water depth of culture apparatus setting is realized by regulating described float or described weight.
10, the method for large scale culturing Haematocoocus Pluvialls as claimed in claim 8 and conversion astaxanthin, it is characterized in that described quiet cell collection device comprises second water pump and cultured products holding tank, the import of described second water pump is connected by the end of flexible pipe with the described second unit light bio-reactor, the outlet of described second water pump is connected with described cultured products holding tank by flexible pipe, and described second water pump will be deposited on the quiet cell harvesting of described second unit light bio-reactor bottom in described cultured products holding tank.
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Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101245310B (en) * | 2008-03-17 | 2011-07-20 | 大连汇新钛设备开发有限公司 | Series connected type unit cell algae optical biological reaction device |
| CL2008001699A1 (en) | 2008-06-09 | 2010-02-05 | Univ Chile | DNA sequence encoding enzyme with cytochrome p450 reductase activity of x. dendrorhous; encoded polypeptide sequence; vector or plasmid; hesped cell; polypeptide production process; astaxanthin production process from beta-carotene; compositions, products and / or formulations. |
| CN102604836B (en) * | 2012-03-01 | 2015-01-21 | 武汉凯迪工程技术研究总院有限公司 | Production method and device for preventing chytrid pollution in haematococcus pluvialis |
| CN102827766B (en) * | 2012-08-17 | 2014-06-18 | 宁波红龙生物科技有限公司 | Photobioreactor for large-scale production of astaxanthin by haematococcus pluvialis |
| CN103396951B (en) * | 2013-08-12 | 2015-03-25 | 云南爱尔康生物技术有限公司 | Method for cultivating haematococcus pluvialis in large scale and producing natural astaxanthin seasoning packet by haematococcus pluvialis |
| CN106906138B (en) * | 2017-05-05 | 2023-08-29 | 宁波浮田生物技术有限公司 | Device and method for inducing astaxanthin accumulation in haematococcus pluvialis at high concentration |
| CN107641594B (en) * | 2017-11-27 | 2020-12-29 | 常德炎帝生物科技有限公司 | Differential pressure type open pipeline bioreactor for microalgae culture |
| CN112280652A (en) * | 2020-10-27 | 2021-01-29 | 南京师范大学镇江创新发展研究院 | Flowing type microalgae photobioreactor based on light energy gradient utilization |
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| CN1226407C (en) * | 2002-07-18 | 2005-11-09 | 中山大学 | Method for improving haematococcus pluvialis multiplication to produce pure strain in photo-biological reactor |
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