CN2763279Y - Gas-lifting type photo-bioreactor for rain-growth red spherical chlorella high density culture - Google Patents

Abstract

The utility model discloses an air-lift photobioreactor for high-density cultivation of Haematococcus pluvialis, which comprises a cultivation tank, a water treatment device, a lighting device and a gas supply device, and is characterized in that the water treatment device is located outside the cultivation tank, The lighting device is located on the side of the breeding tank. The lighting device includes a built-in light source and an external light source. The temperature control device is located at the bottom of the breeding tank. bottom of the tank. The utility model has simple structure, convenient operation, saves production cost, reduces labor intensity, realizes all-weather high-density cultivation by adjusting and controlling reaction conditions, and combines algae cultivation and astaxanthin accumulation in the same reactor to complete.

Description

An airlift photobioreactor for high-density cultivation of Haematococcus pluvialis

technical field

The utility model relates to the field of biological technology, and more specifically relates to an air-lift photobioreactor for high-density cultivation of Haematococcus pluvialis, which is applied to the high-density cultivation of Haematococcus pluvialis and the accumulation of astaxanthin process.

Background technique

Haematococcus pluvialis is a single-cell microalgae, which belongs to Chlorophyta, Volvox, Haematococcus, and Haematococcus in taxonomic status. It can accumulate a large amount of astaxanthin at a specific stage of growth, and is a promising microalgae. As an economical microalgae, it has the following advantages: 1) High economic value, according to the current international market price, the price of 1 kg of dry algae powder is about 20 US dollars or more; 2) The cost of breeding is low, only a small amount of nutrient salt and Certain temperature and light conditions; 3) As freshwater algae, Haematococcus cultivation does not have the environmental pollution problems caused by the discharge of waste liquid from Spirulina and Salina.

The broad market prospect of Haematococcus pluvialis has also attracted widespread international attention. At present, the technology of producing and extracting natural astaxanthin from Haematococcus pluvialis has made great progress abroad. There are companies in Sweden that use artificial sources to irradiate photobiological reactor for production. Aquasearch in the United States cultivates algae in a computer-controlled outdoor closed photobioreactor (AGM), while cyanotech uses a closed "platform" photobioreactor for cultivation. However, there is still a certain distance between the research on this algae and the commercial production at present. There are mainly the following problems in the production: (1) Haematococcus pluvialis is sensitive to environmental changes, has a short exponential growth period, and is resistant to bacteria and native algae. The ability of animals to pollute is poor, and they will lose their reproductive capacity in extreme environments, and it is difficult to establish a stable and efficient production technology system; (2) The cultivation period is long, the yield is low, the continuity is poor, and a large number of deaths occur during the stress process, which is not conducive to large-scale production. scale cultivation. (3) When cultivated in a large pond, the utilization rate of light energy is limited, which restricts the production efficiency in the accumulation stage of astaxanthin and increases the production cost. The above problems hinder the development of this algae and greatly reduce its commodity value. For this reason, people are actively looking for high-density cell culture conditions and ecological regulation techniques for astaxanthin accumulation.

The applicant noticed that the patent application No. 02134385.3 published by the China Intellectual Property Office on January 22, 2003, titled the method for regulating the population density of Haematococcus and the synthesis and accumulation of astaxanthin by a photobioreactor, was developed using an open A large-scale photobioreactor regulates the population density of Haematococcus and the synthesis and accumulation of astaxanthin in cells. The study found that this kind of open reactor has similar defects and difficulties to the extensive production of microalgae in the open air in large ponds, such as a large area, high infrastructure costs, waste of nutrients and energy consumption, and can only be carried out due to the influence of climate. Seasonal production, and the yield, quality and stable production possibility of algae products are threatened by external chemical factors and biological pollution. Some reactors designed in addition have complex technical operations and high investment costs, and some ignore the demand for temperature and light in the algae cultivation process, such as the internal temperature control problem of closed-circuit pipeline reactors, when the algae cell density increases The problem of light supply, etc., and the materials used are easy to age under high temperature and ultraviolet rays, which limit the feasibility of its production and application.

Contents of the invention

The purpose of the utility model is to provide an air-lift photobioreactor for high-density cultivation of Haematococcus pluvialis, which can effectively control the growth of algae cells, solve the problems of algae cell attachment and dissolved oxygen accumulation in the closed cultivation process, and improve Light energy and nutrient utilization efficient farming device. The device combines the two processes of mass cultivation of algae cells and accumulation of astaxanthin in the same container through the adjustment of light intensity, which reduces the loss of algae biomass caused by the intermediate link, and avoids the impact of miscellaneous algae, germs and insect pests. production interference, while facilitating harvesting, reducing labor intensity and saving production costs.

The technical scheme of the utility model is:

An air-lift photobioreactor for high-density culture of Haematococcus pluvialis, comprising a culture tank, a water treatment device, a temperature control device, a gas supply device, a lighting device, a sampling air hole and an algae liquid collection port. It is characterized in that most of the water treatment device is located outside the breeding tank, the lighting device is located on the side of the breeding tank, the lighting device includes a built-in light source and an external light source, the temperature control device is located at the bottom of the breeding tank, and the temperature control device is a gas supply device. The air outlet is located inside the top of the reactor, and the algae liquid collection port is located at the bottom of the culture tank.

The breeding tank is cylindrical, made of resin glass material with good light transmission, 0.3-0.8 meters in diameter, 1.0-1.5 meters in height, and 0.5-1.0 centimeters in thickness of the light-transmitting glass.

The water treatment device includes a filter, an electric heater, an ozone generator and an ultraviolet lamp. Except for the ultraviolet lamp, other components are located outside the reactor; the filter is installed on the water inlet, and the filter material is activated carbon or nano functional material. Its function is mainly to filter the aquaculture water to remove some impurities and coarse suspended particles. The electric heater is installed at the bottom of the reservoir, which can heat the incoming water to 100°C to achieve sterilization. The ozone generator passes through the conduit and The reservoirs are connected, and the ozone generated by it can kill algae and other microorganisms in the water body, ensuring the cleanness and purity of the cultivation water. The ultraviolet lamp is independently installed on the top of the cultivation tank. Different power specifications are used according to the needs. The ultraviolet lamp can further treat the cultivation water. The above measures ensure that the water meets the cultivation needs of the algae.

The lighting device includes two types of built-in light source and external light source, which provide different light intensities required for the algae cell culture stage and the astaxanthin accumulation stage. The built-in light source adopts waterproof submersible fluorescent lamps, each 2-3 as a group, and 2-4 groups are evenly distributed, the submersible fluorescent lamps are installed inside the breeding tank through bolts or vacuum suction cups; One group, 4-6 groups are evenly distributed, fluorescent lamps are installed on the inner side of the reactor shell through bolts or vacuum suction cups; the internal and external light sources of each group can be switched independently, and the combination of light sources can be determined according to the actual situation of cultivation. When the density of algae increases significantly, the light intensity is gradually increased, and all lights can be turned on after the biomass cultivation is completed, so that the algae cells can accumulate astaxanthin under high light. Evenly lay tinfoil paper with good reflective performance on the inner side of the reactor shell. The tinfoil paper occupies 3/4 of the surface area of the shell, leaving 1/4 of the surface area to observe the operation of the reactor during the production process; the tinfoil paper helps to increase the number of breeding tanks Intensity of light within.

The temperature control device at the bottom of the breeding tank consists of two parts. The air-conditioning compressor is outside the breeding tank, and the heat dissipation/heat absorbing device for temperature adjustment is located inside the breeding tank. Connected to air conditioner compressor.

The gas supply device is composed of gas stone, filter, flow meter, gas valve, air pump and carbon dioxide cylinder, and the parts are connected by silicone tubes; the gas filter can be a 35-50 mm syringe filter, and the inside is a pore size 0.30-0.65 micron mixed cellulose ester microporous membrane. The gas in the air pump and the carbon dioxide cylinder is mixed before entering the gas valve. The mixed gas enters the culture tank through the gas valve, flow meter, filter and air stone. The air stone is fixed on the heat dissipation/heat absorption device. connect. One air stone is located on the center of the bottom circle of the culture tank, and the other 6-8 air stones are evenly distributed on the bottom circumference of the culture tank. Provide the gas needed in the culture process, especially CO ₂ , and at the same time make the algae cells and the culture medium fully mixed and contacted through the air flow, and ensure that most cells can receive light evenly.

The sampling air hole is a circular opening with a diameter of 30-60 mm on the top of the culture tank, and a detachable gas filter containing a mixed cellulose ester microporous membrane is installed, and the filter is connected to the culture tank by a sealing ring. The hole is used for the escape of the oxygen produced by the algae cells in the reactor to balance the air pressure in the reactor. After the filter is pulled out, the hole can also be used for adding the culture mother solution, inoculating the algae species, sampling the analysis sample during the culture process, and measuring the temperature and light intensity of the reaction chamber.

The principle of the utility model is: 1. Thoroughly treat the water for cultivation through filtration, heating, ozone treatment and ultraviolet irradiation, which ensures the safety and sanitation of water, and effectively avoids the harm of miscellaneous algae, germs and protozoa; 2. In production application The aeration of the air stone drives the mixed movement and close contact between the culture medium and the algae cells, which ensures the uniformity of the culture environment to the greatest extent, promotes the utilization of nutrients and light energy by the algae cells, and improves the mass transfer efficiency; 3. According to the cell The actual needs of the growth stage decided to turn on different light source combinations to ensure that both the algal cell growth and the astaxanthin accumulation stage can get a suitable light intensity, so that the algal cells in the culture tank will not have insufficient light energy due to the increase in density. It is beneficial for algal cells to obtain high-density biomass, and then directly enters the astaxanthin accumulation stage, prompting algal cells to form thick-walled spores and accumulate astaxanthin.

The technical characteristics and advantages of this bioreactor:

1. Clean and pollution-free. The reactor adopts a filter device for the cultivation water and gas, which effectively prevents the entry of impurities and bacteria in the air and water. In addition, the closed breeding mode effectively prevents the invasion of algae and protozoa.

2. The culture density is high. The reactor is equipped with internal and external light sources, which effectively prevents the self-shading effect after the culture density is high, ensures sufficient supply of light energy, and helps to achieve high-density culture.

3. A stable and efficient production technology system is provided. The reactor is equipped with heat dissipation/heat absorption devices in the culture tank, while the air conditioner compressor is located outside the culture tank. This design can ensure that the growth and reproduction of algae cells is always at In the most suitable temperature range, in addition, due to the sufficient supply of light, and the reactor solves the interference of algae and insect pests, etc., the smooth progress of the cultivation is ensured.

4. The technological process is simplified. In the past, a large number of green cells were cultivated first, and then these algae cells were placed in a stressful environment for 2-3 days to transform them into chlamydospores and accumulate astaxanthin; this reactor The unique design simplifies the traditional process, reduces the potential loss of biomass due to intermediate links, and at the same time helps to accumulate astaxanthin and improve product quality.

5. The design is simple, the operation is simple and easy, and at the same time, it does not pollute the environment, and has production and application value.

Description of drawings

Fig. 1 is a structural schematic diagram of an airlift photobioreactor for high-density cultivation of Haematococcus pluvialis.

Detailed ways

Example:

The utility model is described in further detail below according to the accompanying drawings:

An airlift photobioreactor for high-density cultivation of Haematococcus pluvialis, which includes a culture tank 2, a water treatment device 6, a temperature control device 5, a gas supply device 4, a lighting device 3, a sampling air hole 7 and algae Liquid collection port 8.

Breeding tank 2 is cylindrical, adopts the good resin glass material of translucency to make, and the specification that present embodiment adopts is diameter 0.5 meter, height 1.5 meter, and the thickness of translucent glass is 0.6 centimetre. There are sampling vents 7 and ultraviolet lamps 19 on the top of the breeding tank 2, a water treatment device 6 on the upper left, an illumination device 3 in the middle, an air supply device 4, a temperature control device 5 and an algae liquid collection port 8 at the bottom.

Water treatment device 6 comprises ultraviolet lamp 19, reservoir 20, water inlet filter 21, electric heater 22 and ozone generator 23, except ultraviolet lamp 19, other parts are all positioned at outside the reactor; Water inlet filter 21 is installed on On the water inlet, the filter material is activated carbon, and its function is mainly to filter the breeding water to remove some impurities and coarse suspended particles. , can heat the incoming water to 100°C to achieve sterilization, the ozone generator 23 (ozone generation 5g/h) is connected to the reservoir 20 through a conduit, and the ozone generated by it can kill algae and other microorganisms in the water body. The filtered water is first heated to 100°C, and then the ozone generator is turned on to kill microorganisms in the water body through the action of ozone to ensure the cleanness and purity of the water for cultivation. Ultraviolet lamps 19 (30W, 3) are independently installed on the top of the cylindrical culture tank 2, and the ultraviolet lamps 19 are turned on for 24 hours after the cultivation water and the cultivation mother liquor are added to further treat the cultivation water; the parts are not connected to each other, But their common function is to ensure that the water meets the cultivation needs of the algae.

The temperature control device 5 is located at the bottom of the breeding tank 2 and consists of two parts: a heat dissipation/heat absorption device 17 and an air conditioner compressor 18. The air conditioner compressor 18 is outside the cultivation tank 2, and the heat dissipation/heat absorption device 17 for temperature regulation is located in the cultivation tank 2 Inside, it is installed on the bottom of the breeding tank 2 by waterproof gaskets and bolts, and is connected with the air-conditioning compressor 18 by a draft tube.

Gas supply device 4 is made up of gas stone 11, flow meter 12, filter 13, air valve 14, air pump 15 and carbon dioxide cylinder 16, and each part is connected in series by silica gel tube; the gas in air pump 15 and carbon dioxide cylinder 16 Mix before entering the air valve 14, the mixed gas enters the cultivation tank 2 through "air valve 14→flow meter 13→filter 12→air stone 11"; the filter 13 can be a 35 mm syringe filter with an internal diameter of 0.30 Micron mixed cellulose ester microporous membrane. The air stone 11 is fixed on the circular cooling/heat absorbing device 17, one air stone is located on the center of the circle at the bottom of the culture tank 2, and the other 6-8 air stones are evenly distributed on the circumference of the bottom of the culture tank 2. Use separate connection between. This sub-device provides the gas needed in the culture process, especially CO ₂ , and at the same time makes the algal cells and the culture solution fully mixed and contacted through the air flow, and ensures that most cells can receive light evenly.

The lighting device 3 is divided into two types: built-in light source and external light source; the built-in light source adopts waterproof submersible fluorescent lamp 9, the power is 20W, every 2 is a group, and 3 groups are evenly distributed inside the breeding tank 2; the external light source adopts fluorescent lamp 10, The power is 20W, and each group of 2 is a group, and 4 groups are evenly distributed inside the reactor shell 1; these lamps provide light energy with a light intensity ranging from 30 to 350 μmol m ^-2 s ^-1 for photosynthesis of algae cells. Each group of internal and external light sources can be switched independently, and the combination of light sources can be determined according to the actual situation. Evenly lay tinfoil paper with good reflective performance on the inner side of the reactor shell 1. The tinfoil paper occupies 3/4 of the surface area of the shell, leaving 1/4 of the surface area to observe the operation of the reactor during the production process. The lighting device 3 is installed in the center of the reactor, and the lamps are connected in parallel, and are respectively fixed on the culture tank wall and the reactor shell 1 .

The sampling air hole 7 is a circular opening with a diameter of 50 mm, located inside the top of the reactor, and a detachable 0.30 micron pore size mixed cellulose ester microporous filter membrane is installed for the oxygen produced by the reactor algae cells to escape and balance the reactor air pressure inside. The hole is also used for the addition of the culture mother solution, the inoculation of the algae species, the sampling of the analysis samples during the culture process, and the determination of the temperature and light intensity of the reaction chamber.

Claims (5)

1, a kind of airlift photobioreactor of Haematocoocus Pluvialls high-density culture, comprise cultivation tank (2), water treatment device (6), means of illumination (3), gas supply device (4), it is characterized in that water treatment device (6) is positioned at cultivation tank (2) outside, means of illumination (3) is positioned at cultivation tank (2) side, means of illumination (3) comprises built-in light source and external light source, temperature-control device (5) is positioned at cultivation tank (2) bottom, on temperature-control device (5) gas supply device (4), sampling production well (7) is positioned at the reactor head inboard, and algae liquid is collected mouthful (8) and is positioned at cultivation tank (2) bottom.

2, the airlift photobioreactor of a kind of Haematocoocus Pluvialls high-density culture according to claim 1, it is characterized in that water treatment device (6) comprises strainer (21), electricradiator (22), ozonizer (23) and ultraviolet lamp (19), strainer (21) is installed on the water-in, electricradiator (22) is installed in the bottom of water reservoir (20), ozonizer (23) links to each other with water reservoir (20) by conduit, and ultraviolet lamp (19) is installed in cultivation tank (2) inner top.

3, the airlift photobioreactor of a kind of Haematocoocus Pluvialls high-density culture according to claim 1, it is characterized in that temperature-control device (5) is made up of heat radiation/heat-absorbing device (17) and compressor of air conditioner (18), compressor of air conditioner (18) is positioned at cultivation tank (2) outside, heat radiation/heat-absorbing device (17) is positioned at cultivation tank (2) inside, be installed in cultivation tank (2) bottom by waterproof washer and bolt, and link to each other with compressor of air conditioner (18) by thrust-augmenting nozzle.

4, the airlift photobioreactor of a kind of Haematocoocus Pluvialls high-density culture according to claim 1, it is characterized in that gas supply device (4) is made up of gas stone (11), under meter (12), strainer (13), air valve (14), pneumatic pump (15) and carbon dioxide steel cylinder (16), and be connected in series with sebific duct successively.

5, the airlift photobioreactor of a kind of Haematocoocus Pluvialls high-density culture according to claim 1, it is characterized in that two kinds of means of illumination (3) branch built-in light source and external light sources, built-in light source adopts waterproof submersible type luminescent lamp (9), per 2 is one group, and three groups are evenly distributed on cultivation tank (2) inside; External light source adopts luminescent lamp (10), and per 2 is one group, four groups of inboards that are evenly distributed on shell of reactor (1); Means of illumination (3) is installed on the middle position of reactor, for being connected in parallel, and being separately fixed at and culturing on tank skin and the shell of reactor (1) between each lamp.