Background technology
The microalgae microorganism is a kind of novel protein resource that has obtained broad research and exploitation since 20th century in the whole world.Contain the nutrition of multiple human body needs such as very high protein, amino acid, VITAMIN, unsaturated fatty acids, polysaccharide and mineral substance in little algae.From the nineties in last century so far, little algae industry of China such as the former powder annual production of spirulina are all more than 1000 tons.Since 21 century Katyuan, people are newfound to bio-science man, have the very high healthy blood cell algae natural astaxanthin that is worth expresses great concern.Astaxanthin 3S-3 ' S that this algae is contained, Astaxanthin are the product innovations of the most breathtaking little algae biotechnology industry of research and development in nearest 10 years.3S-3 ' S astaxanthin is that the blood cell algae carries out photosynthetic response in intense light irradiation, from the further synthetic end product of its β-Hu Luobusu, has very highly purified optically active chiral structure, confirmed as a kind of powerful 10 times by biochemist and nutritionist, than the powerful 550 times super VITAMIN of vitamin-E than β-Hu Luobusu.
But so far on the mode of production of little algae,, still adopting open-air Da Chi production system to carry out the suitability for industrialized production of little algae as the Earthrise spirulina company of the U.S., the little algae of Cyanotech company, the little algae of Japanese DIC company etc.; Promptly produce as little algae blood cell algae newly developed in recent years, as famous little algae manufacturer such as the Mera Pharmaceuticals company of the U.S. and Cyanotech company, large-scale industrial production in this algae is technical, promptly in production phase frustule second phase, still adopts Da Chi to produce in the open.This extensive style production of carrying out little algae with Da Chi exists many defectives and difficulty, and is excessive as floor space, the capital construction cost is very high, the fertilizer input is many with the energy consumption waste, and be subjected to weather effect can only carry out seasonality production; The more important thing is that the Da Chi cultivation is polluted by extraneous chemokines and heteroplasia thing very easily, down to quality that has influence on the algae product and stably manufactured etc.For overcoming these limiting factors, since the eighties, numerous biotech specialists and biotechnology development company have been arranged both at home and abroad, tested the various all kinds of sealings algae photosynthetic organism reactor that declines, in the hope of replacing the Da Chi mode of production.A wherein representational class such as level land duct type structure photosynthetic organism reactor (people 1993 such as Torzillo, people such as Weissman 1987); The 2nd, plate armature class photosynthetic reactor (people 1996 such as Hu, people such as Pulz 1995); The 3rd class is a drum type optical fiber photosynthetic organism reactor, adopts photoconductive fiber to transmit in reactor and dispersion luminous energy people 1991 such as () Javanmardian, and this class reactor makes the efficiency of light energy utilization of frustule obtain large increase.Meanwhile, more domestic scientific researches and production unit are also attempted development and are replaced popular Da Chi mode with closed photosynthetic reactor and carry out little algae and produce.As a kind of SPIRULINA CULTIVATION mode that adopts transparent plastic film tube and in pipeline, ventilate; Also there are modes such as the glass jar of employing, sheet glass tank to cultivate little algae or the like.Although the research of above-mentioned all kinds of photosynthetic organism reactors and manufacturing, aspect the illumination and nutrition utilization ratio in cultivating and produce for little algae, different characteristic and advantages of each tool all, but can only be to support utilization aspect laboratory and little, the pilot scale production so far, far can not expand to the application of getting on of little algae suitability for industrialized production.Hindering the move towards the industrialization major obstacle of production application of little algae photosynthetic organism reactor, at first is the technological operation aspect complexity of above-mentioned all kinds of photosynthetic organism reactors, and cost of investment is too high, can't carry out big water body production; Wherein the most difficult is, and frustule carries out photosynthetic product oxygen in pipeline and the dissolved oxygen that causes is accumulated and is difficult to get rid of; Next is that interior temperature is difficult to control in the closed type pipeline; It three is that the wall contact scar of being stained with that takes place on the inner-walls of duct has a strong impact on optical throughput.In addition, the employing plastic film pipeline that has carries out little algae to be cultivated, and under summer high temperature and ultraviolet irradiation, aging soon collapsing collapsed.So on little algae industrialization was produced, the example that the photosynthetic organism reactor is applied to large-scale industrial production still was not reported so far.
Summary of the invention
The objective of the invention is to solve and overcome at present and carrying out little algae cultivation both at home and abroad, especially the structural limiting factor on the mcroorganism amount suitability for industrialized production and the technology barrier of generation thereof, design and to produce a kind of floor space few, can effectively improve the efficiency of light energy utilization, effective controlled temperature, can effectively solve the wall of being stained with in the closed conduit, can effectively get rid of ducted dissolved oxygen and accumulate, can in time supply with and make full use of CO
2, can carry out the little algae photosynthetic organism of mcroorganism amount industrial continuous production reactor assembly with big water body, multiple-unit combination.
Technical scheme of the present invention is:
A kind of little algae suitability for industrialized production photosynthetic organism reactor assembly, comprise reaction tower, little algae photosynthetic organism reacting pipe device, infusion pump, the carbonic acid gas gas injection device, the artificial lighting device, cool-heat-exchanger, tubing, it is characterized in that: reaction tower adopts the Double-Tower Structure of parallel upright, deoxygenation reaction tower top is provided with negative pressure pump, and the negative pressure pump side is provided with the oxygen venting port, and deoxygenation reaction tower middle part is a cavity, deoxygenation reaction tower bottom is connected with regulation and control reaction tower bottom, regulation and control reaction tower middle part is a cavity, and the connection suitable for reading of negative pressure-pumping Guan Yuwei algae photosynthetic organism reacting pipe device is arranged at regulation and control reaction tower top, and regulation and control reaction tower side is provided with the carbonic acid gas fillup valve and communicates with the carbonic acid gas gas injection device, little algae photosynthetic organism reacting pipe device is provided with the pipeline cleaning valve, the frustule valve of gathering, the bottom of little algae photosynthetic organism reacting pipe device is connected with cool-heat-exchanger, and cool-heat-exchanger is connected with infusion pump, and infusion pump communicates by the negative pressure pump of tubing with the top of deoxygenation reaction tower.
More excellent technical scheme is just like following:
Regulation and control reaction tower top also is provided with hydraulic pressure and air pressure regulation and control mouth.
Deoxygenation reaction tower bottom also is provided with the reactant sampling valve with regulation and control junction, reaction tower bottom.
Little algae photosynthetic organism reacting pipe device adopts three-dimensional double flat spin formula plumbing installation, and every layer of assembly of elements of the double flat spin formula of its neutral body plumbing installation is straight tube, adopts isometrical U type connecting bend to connect.
Deoxygenation reaction tower, regulation and control reaction tower and little algae photosynthetic organism reacting pipe device are made of high transparent glass material.
Infusion pump adopts zero shearing force nutrient solution driving pump.
The two negative pressure pump structures of two channels are adopted on deoxygenation reaction tower top, and the two negative pressure pump sides of two channels are provided with the oxygen venting port.
The present invention has adopted the little algae photosynthetic reactor of closed conduit system, fully used the principle of little algae photosynthetic organism reaction, in conjunction with the stechiology characteristics, applying biological environment theory of controls and modern biophysics new technology, adopting high borosilicate enhancement type glass pipe is main raw, test by design repeatedly, field fabrication and by production application, the vertical totally-enclosed glass pipe photosynthetic organism reactor of a kind of ultra-large type (Vertical Glass Photobioreactor, VGPR) system have been invented.VGPR of the present invention is the unit-combination type system, can be formed directly in the suitability for industrialized production workshop by the multiple-unit combination.
VGPR of the present invention system has fully optimized in little algae production process for luminous energy, temperature, nutrition utilization, comprise all frustule physiology growth conditionss such as pH regulation and control, deoxygenation, carbonic acid gas supply and duct cleaning, to have overcome conventional little algae mode of production floor space big comprehensively, energy consumption is big, productive rate is low, seriously polluted, and the drawback that can not in all weather of the year grow; The obstacle that the breakthrough photosynthetic organism reactor that has solved under the international and domestic various experiment condition can not be upgraded and be amplified and measure suitability for industrialized production as mcroorganism.VGPR system of the present invention has realized the comprehensive natural light that utilizes owing to be three-dimensional arrangement, adopts the secondary light source light filling when overcast and rainy illumination is not enough, can reach energy-conservation purpose; Nutrient solution is turbulence type and flows in pipeline, can make frustule be in high-frequency light/in the dark cycle, and high-level efficiency provides frustule required photon when carrying out photosynthetic response; Carbonic acid gas is imported in the metering mode of coercing, and fully satisfies the needs of frustule to carbonic acid gas; Reaction tower is equipped with negative pressure pump, can get rid of the dissolved oxygen that culturing cell takes place in photosynthetic response timely and effectively, thereby has greatly improved photosynthetic efficiency; Because adopted the driving pump of zero shearing force, frustule preserves from when turning round in system; Also, frustule is in the optimum temps, makes biological yield and output obtain stable significantly raising owing to be provided with the cool-heat-exchanger controlled temperature; This VGPR system can stop infecting of all the extraneous heteroplasia things and the various chemical pollution factors owing to be that totally enclosed circulation continuous production is cultivated, and is specially adapted to large-scale industrialized production higher-grade food, the little algae product of pharmaceutical grade.
The reaction tower of VGPR system adopts the Double-Tower Structure of parallel upright, and the two negative pressure pumps of two channels are adopted on deoxygenation reaction tower top, and two negative pressure pumps side respectively is provided with the oxygen venting port, can constantly get rid of the oxygen of accumulating in the nutrient solution in the operation process of nutrient solution; The regulation and control reaction tower is equipped with carbonic acid gas gas injection valve, coerces carbonic acid gas and directly enters in the glass pipe of three-dimensional double flat spin formula plumbing installation and participate in photosynthetic response; Nutrient solution comprises new configuration nutrient solution and gathers and reclaim liquid by injecting on the reaction tower; The frustule valve of gathering is expelled to filter receipts machine, the spray-dried formation product of the algae mud of gathering under the reacting pipe; The double flat spin formula plumbing installation of solid can adopt 20~100 millimeters of internal diameters, and the straight tube that length overall is 40~300 meters connects with isometrical U-shaped glass bend pipe, realizes that nutrient solution turbulent flow in pipeline takes place and the drop gravity flow is mobile from top to bottom; The driving pump of zero shearing force is housed under the reaction tower, pumps nutrient solution continuously, and cooperate formation hydraulic pressure negative pressure, within the regulation and control reaction tower, manage the suitable for reading of automatic sucting reaction tubing system with the regulation and control reaction tower from the bottom portion of pipe reactor.Be provided with cold and hot exchange temperature control unit in the tubing system bottom; Microwave sulfur lamp is installed as light energy source in top and both sides in the VGPR system, and luminous intensity can require to regulate according to the growth of frustule physiology; Pipeline adopts the physics cleaning way, can produce and exempt to dismantle under the situation of pipeline not shutting down, and that removes whole tubing rapidly is stained with wall and other pollutent, and outside special use outlet discharge system.
Double-Tower Structure is a main innovate point of the present invention, and double tower cooperates effectively deoxygenation and effectively input carbonic acid gas simultaneously, particularly can regulate liquid level and keep hydro-cushion; Double-Tower Structure has also guaranteed behind most of little algae, can stay the algae kind usefulness that the little algae of part is cultivated as next round by a certain percentage in having gathered in the crops system, has quickened cultivation and the serialization production process of little algae.
The negative pressure squirt pump is established on deoxygenation reaction tower top, cooperates infusion pump to form lower negative pressure, helps getting rid of the dissolved oxygen in the nutrient solution, improves the efficient of photosynthetic response; Regulation and control reaction tower both pumped as the hydraulic pressure negative pressure and the photosynthetic response plumbing installation between cushioning pocket, again various dynamic regulations and monitoring equipment can be installed; Hydraulic pressure that is provided with and air pressure are regulated and control mouth, can regulate the liquid level of two reaction towers better; Carbonic acid gas gas injection valve is arranged on the side of regulation and control reaction tower, because driving pump constantly extracts nutrient solution from the reacting pipe bottom, cause the regulation and control tower to produce the hydraulic pressure negative pressure, carbonic acid gas enters little algae photosynthetic response plumbing installation with the negative pressure extracting in the reaction tower, and carbonic acid gas is not easy loss.Be arranged on other positions as carbonic acid gas gas injection valve, though do not influence the work of system, carbon dioxide loss is bigger; Infusion pump adopts zero shearing force nutrient solution driving pump, when conveyance fluid and little algae, frustule is not caused shear fracture.Reactant sampling and bleed valve are also set up with regulation and control junction, reaction tower bottom in deoxygenation reaction tower bottom, help taking a sample at any time cultivating and double tower being cleaned to monitor little algae.
Another innovative characteristics of VGPR system is to adopt three-dimensional double flat spin formula plumbing installation.The unit that the double flat spin formula of its neutral body pipeline is every layer adopts straight tube, connects with isometrical U type elbow.U type connecting bend is the plane slight inclination relatively, and the connection that two adjacent straight tubes can be misplaced up and down makes whole little algae photosynthetic response plumbing installation be three-dimensional double flat spin form.This simple straight tube, U type connecting bend and unique mode of connection are for making and the erecting equipment particularly advantageous.The levels connecting bend also plays the effect that turbulent flow takes place simultaneously, makes frustule obtain high-frequency light-dark reaction in native system, and photosynthetic efficiency takes this to be greatly improved.
The technical characterictic and the advantage of VGPR of the present invention system:
1. the little algae photosynthetic organism of the present invention reactor (VGPR) system floor space is minimum, and under the situation that obtains identical production scale, its floor space only needs 6% of conventional Da Chi culture systems, and the land tenure expense can be saved more than 80%;
2. can save water, electric equal energy source consumption significantly, under identical production scale condition, the consumption of water is 12% of other production systems such as Da Chi, and the consumption of electricity is 26% of Da Chi production system, raw material (nutrition, CO that algae produces
2Deng input) consume and only to need 40% of the Da Chi mode of production;
3. full-closed structure can be controlled the invasion and the pollution of extraneous chemical and biological foreign matter, and the algae product quality is guaranteed;
4. the equipment investment cost of VGPR of the present invention system is lower than Da Chi and cultivates production system, owing to be that highly intensification is produced, easy and simple to handle, be easy to manage, labour intensity significantly is lower than the Da Chi production management, and its personnel only need 40% of Da Chi culture systems;
5. VGPR system of the present invention is owing to be to produce under the control condition fully, can carry out the whole year, round-the-clock production;
6. system of the present invention is owing to can make frustule carry out high efficiency photosynthesis, and the various nutrition of being supplied with can make full use of, and productive rate of algae and yield improve 3-5 doubly than the open cultivation of Da Chi;
7. system of the present invention forms the suitability for industrialized production scale with unit-combination type, and as with 50 unit combination, the annual production of algae is equivalent to 8000 meters of Da Chi production systems
2Industrial scale.The cultivation of algae is with both gather can be by unit operation as scientific research, middle trial production, but the all-in and all-out mode of production is taked in multiple-unit combination again, realizes carrying out little algae mcroorganism amount industrialization production with the workshop form;
8. system of the present invention has been owing to adopted the hydraulic principle design of science, high-strength transparent material, and used and be different from the Da Chi production formula, be not prone to therefore that Da Chi produces and other type photosynthetic reactor in the normal incrustation that takes place and be stained with wall.VGPR of the present invention system is in use in case there is the wall of being stained with phenomenon to take place in pipeline, and the available physical cleaning way cleans up whole tubing systems, and no residual hazard and residue do not influence continuous production.
9. the present invention has military significance, VGPR unit facility can be in office where territory such as island, high mountain install and use, produce high-quality, the little algae nutritive foodstuff of high protein, and simple to operate, very big strategic importance is arranged;
10. VGPR of the present invention system is particularly suitable for carrying out the orientation of little algae and coerces cultivation, can produce polyunsaturated fatty acid, specified vitamin by the hot housing frustule, can the multiple important mineral element of organic complex such as rich iodine, rich selenium, rich germanium, little algae products such as Fu Tie, Fu Xin, it is incomparable that this is that Da Chi produces;
11. the present invention can be implemented under the strict control condition and produces, no waste gas is only discharged oxygen, but the purifying environment, no waste liquid, nutrient solution can reuse, and only needs to replenish fresh nutritive substance, does not have other harmful side product.More because little algae protein abundance, pufa-containing and multivitamin, and high yield high-quality characteristics such as frustule is with short production cycle, reproduction speed is fast, from the long term growth strategic concerns, the present invention is for the biotechnology Green Revolution that realizes little algae, develop new food resource, ensure that human life with healthy, has great strategic importance and applied economics meaning.
Embodiment
Embodiment 1
A kind of little algae suitability for industrialized production photosynthetic organism reactor assembly, comprise reaction tower, little algae photosynthetic organism reacting pipe device 2, infusion pump 8, the carbonic acid gas gas injection device, artificial lighting device 13, cool-heat-exchanger 14, tubing 1, reaction tower adopts the Double-Tower Structure of parallel upright, deoxygenation reaction tower 4a top is provided with negative pressure pump 9, negative pressure pump 9 sides are provided with oxygen venting port 10, deoxygenation reaction tower 4a middle part is a cavity, deoxygenation reaction tower 4a bottom is connected with regulation and control reaction tower 4b bottom, the junction also is provided with reactant sampling valve 7, regulation and control reaction tower 4b middle part is a cavity, the suitable for reading connection of negative pressure-pumping pipe 12 and little algae photosynthetic organism reacting pipe device 2 arranged at regulation and control reaction tower 4b top, regulation and control reaction tower 4b top also is provided with hydraulic pressure and air pressure regulation and control mouth 11, regulation and control reaction tower 4b side is provided with carbonic acid gas fillup valve 5 and communicates with the carbonic acid gas gas injection device, little algae photosynthetic organism reacting pipe device 2 is provided with pipeline cleaning valve 6, the frustule valve 15 of gathering, the bottom of little algae photosynthetic organism reacting pipe device 2 is connected with cool-heat-exchanger 14, and cool-heat-exchanger 14 is connected with infusion pump 8, and infusion pump 8 communicates by the negative pressure pump 9 of tubing 1 with the top of deoxygenation reaction tower 4a.
Embodiment 2
A kind of little algae suitability for industrialized production photosynthetic organism reactor assembly, little algae photosynthetic organism reacting pipe device 2 adopts three-dimensional double flat spin formula plumbing installation, every layer of assembly of elements of the double flat spin formula of its neutral body plumbing installation is straight tube, adopts isometrical U type connecting bend 3 to connect; Deoxygenation reaction tower 4a, regulation and control reaction tower 4b and little algae photosynthetic organism reacting pipe device 2 are made of high transparent glass material.Infusion pump 8 adopts zero shearing force nutrient solution driving pump; Two negative pressure pump 9 structures of two channels are adopted on deoxygenation reaction tower 4a top, and two negative pressure pump 9 sides of two channels are provided with oxygen venting port 10.
All the other structures such as embodiment 1.
Its operational process is as follows: a kind of zero shearing force nutrient solution driving pump is installed under the VGPR system, infusion pump 8 is with the nutrient solution in the reacting pipe, inject negative pressure pump 9 by tubing 1, nutrient solution injects its underpart deoxygenation reaction tower 4a with spray regime, in this process, the supersaturation oxygen that frustule produces in photosynthetic response passes through outside the oxygen venting port 10 discharge systems with negative pressure; As untimely eliminating, the oxygen partial pressure pO in the nutrient solution
2Can reach 400%, VGPR of the present invention can control pO
2Below 100%, the nutrient solution that has discharged oxygen enters regulation and control reaction tower 4b by the connection bend pipe of double tower bottom; Reactant sampling valve 7, in order to the optical density(OD) and the nutraceutical consumption situation of sample examination frustule growth, reactant sampling valve 7 is gone back the double as reaction tower and is cleaned, changes purposes such as liquid; Nutrient solution from the negative pressure-pumping pipe 12 on regulation and control reaction tower 4b top by auto-pumping to little algae photosynthetic organism reacting pipe device 2; Carbonic acid gas is coerced at carbonic acid gas gas injection valve 5 places pumps into little algae photosynthetic organism reacting pipe device 2, as the carbon nutrition of frustule in carrying out photosynthesis; The total length of reacting pipe of the present invention can connect between 40~300 meters, adjusts according to the production actual needs of biomass, and connecting bend 3 plays the effect that turbulent flow takes place simultaneously up and down, makes frustule obtain high-frequency light-dark reaction in native system.Frustule is originated as photon with natural light when the high illumination of fine day, and illumination is not enough or carry out opening when frustule is coerced cultivation artificial lighting device 13 light source as a supplement.Nutrient solution in the reacting pipe heats up under the high illumination of fine day comparatively fast, and the bottom of VGPR of the present invention system is provided with cold and hot water interchanger 14, in order to control summer high temperature and winter low temperature, frustule is stabilized in the optimum temperuture condition.
The present invention has been owing to optimized the optimal growth condition of microalgae cell such as nutraceutical utilizations such as temperature, illumination, pH, dissolved oxygen level (D.O.) and carbon, nitrogen, phosphorus, can make that frustule is active to carry out growth and breeding, and the cellular biomass increase is very fast.Nutrient solution flows into reaction tower through infusion pump 8 then once more from the bottom pipe that flows to step by step suitable for reading of VGPR reacting pipe, the circulation that goes round and begins again in 24 hours.When cultivating algae and reach certain optical density(OD) or cell concn, can be expelled to the filter of gathering from the frustule of the bottom pipe valve 15 of gathering, algae mud is sent to spraying drying or is done other processing.Filtered liquid still contains partly nutrition, after replenishing fresh nutrition, is pumped go back to the VGPR system once more.The frustule that keeps among deoxygenation reaction tower 4a and the regulation and control reaction tower 4b is enough to become the inoculum that the little algae of next round total system is cultivated.