CN202968563U - Airlift biochemical culture device - Google Patents
Airlift biochemical culture device Download PDFInfo
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- CN202968563U CN202968563U CN 201220660135 CN201220660135U CN202968563U CN 202968563 U CN202968563 U CN 202968563U CN 201220660135 CN201220660135 CN 201220660135 CN 201220660135 U CN201220660135 U CN 201220660135U CN 202968563 U CN202968563 U CN 202968563U
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Abstract
The utility model discloses an airlift biochemical culture device which comprises a quartz bottle body and further comprises a bottle cover arranged on the quartz bottle body, wherein the bottle cover is provided with a gas inlet tube, a gas outlet tube and a sampling tube; one end of the gas inlet tube extends into the bottom of the quartz bottle body and is provided with an air dispersing sieve; the other end of the gas inlet tube is sequentially connected with an air pump at the gas inlet through a first filter and a second filter at the gas inlet; one end of the sampling tube extends into the bottom of the quartz bottle body; the gas outlet tube is connected with the air pump at the gas inlet through the filter at the gas outlet; the gas inlet tube blows air to the quartz bottle body through the air pump at the gas inlet; and the gas outlet tube extracts gas from the quartz bottle body through the air pump at the gas inlet. The airlift biochemical culture device disclosed by the utility model is simple in structure and convenient to operate, and multiple treatments and repeats can be performed at the same time, so that the researching speed and efficiency are greatly improved.
Description
Technical field
The utility model belongs to the biochemical culture technique of Life Science Laboratory field, more specifically relates to the biochemical culture apparatus of a kind of air lift type, the culture studies of applicable autotrophic cell, heterotrophic cell and animal vegetable tissue.
Background technology
Biochemical engineering is one of life science new and high technology direction.Medicine, the food of producing the human needs by the biochemical engineering means are one of life science development trends.In the fundamental research of biochemical reaction, cell cultures is the most frequently used a kind of means of studying wild-type cell and the synthetic purpose product of engineering cell.Yet present cell cultures mode is mainly two kinds: the one, and miniature fermenter is cultivated, and the 2nd, shake-flask culture (namely shaking cultivation with culturing bottle on shaking table).These two kinds of training methods have fatal defective.For traditional fermentor cultivation, experiment repeats few, and control condition is limited, especially can't carry out with the traditional zymotic tank for the photoresponse research of biological metabolism.For shake-flask culture, the gaseous interchange of uncontrollable substratum and gas composition, and also common culturing bottle is that simple glass is made or transparent plastics is made, and can not be used for biological metabolism photoresponse research.In addition, shake-flask culture is subjected to the impact of shaking table size and number, and experiment repeats to be restricted, and has often increased experimental error; Can not carry out photo-irradiation treatment equably to culturing bottle in the shaking table culturing process, thereby shaking table is cultivated the photoresponse research that has also limited biological metabolism.
This device is gone back emphasis and has been solved the experimental technique problem of carrying out the long photoresponse research of all-wave of biological metabolism with cell cultures except the defective that solves at present traditional fermentor tank and shaking flask.The long photoresponse research of the all-wave of biological metabolism became the emerging research field of life science in recent years.The scientific basic of this area research is: the autotrophic organism cell is nourished and grown under the light source irradiation of certain wavelength and the secondary metabolite accumulation all is subject to the orientation adjustment of wavelength, and heterotroph cell pathways metabolism under the stimulation of special wavelength light source also can change and cause the accumulation of some biologically active substances.These characteristics of life cell can be used for suitability for industrialized production human needs's biologically active substance or medicine.Because the different wave length source all possesses certain energy, and can only be absorbed by some special phytochrome or other photoactive substance in life cell (as some enzyme or non-enzymatic protein acceptor) and its physiologically active be strengthened, cause at last corresponding genetic expression or metabolism network to change, cause that thus some specific secondary metabolite expression amount increases.Utilize this characteristic of life cell, can system in depth study light sources with different wavelengths to the Regulation Mechanism of metabolism network, these unknown photoresponse mechanism are important contents of bio-light reaction scientific research, are also the core theory of cell photoresponse technical field in the biochemical engineering high-tech industry.But still do not meet the scientific instrument of these research needs at present.
This research group has applied for being used for the culture apparatus of bio-light repercussion study, a kind of organism culturing device of Full-wavelength light source controllable, application number: 201110424449.7.In order to carry out more easily biological metabolism photoresponse research, need to provide a kind of air lift type biochemical culture apparatus.The organism culturing device of this device and a kind of Full-wavelength light source controllable is used in conjunction with.Organism culturing device with a kind of Full-wavelength light source controllable provides light source and the temperature of reaction of controlling wavelength, as easy bio-reactor, can carry out simultaneously the biological metabolism photoresponse research of a plurality of repetitions of a plurality of processing with the application.Speed of experiment and Efficiency have so greatly been improved.
This device can also be used for controlling by gas and carries out the pure oxygen fermentation research or regulate the air carbon-nitrogen ratio and carry out fermentation research, pass into nitrogen and carry out anaerobically fermenting research and adjustments of gas composition and atmospheric moisture and carry out Study on tissue culture etc. except being used for carrying out biological metabolism photoresponse research.
The utility model content
The purpose of this utility model is the problems referred to above that are for the prior art existence, provides a kind of air lift type biochemical culture apparatus, the metabolism photoresponse research of applicable autotrophic cell, heterotrophic cell and animal vegetable tissue.Can also be used for replacing traditional shaking table to cultivate, controls by gas and can carry out the pure oxygen fermentation research or regulate the air carbon-nitrogen ratio and carry out fermentation research, pass into nitrogen and carry out anaerobically fermenting research, adjustments of gas composition and atmospheric moisture and carry out Study on tissue culture etc. by air-blowing.
To achieve these goals, the utility model is by the following technical solutions:
the biochemical culture apparatus of a kind of air lift type, comprise quartzy bottle, also comprise the bottle cap that is arranged on quartzy bottle, be provided with inlet pipe on bottle cap, escape pipe and stopple coupon, one end of inlet pipe extends to quartzy bottle body bottom and is provided with the air dispersion sieve, the inlet pipe the other end is connected strainer by inlet mouth the first strainer successively and is connected with the inlet mouth air pump with inlet mouth, one end of stopple coupon extends to quartzy bottle body bottom, escape pipe is connected with the air outlet air pump by the air outlet strainer, inlet pipe is by air-blowing in the past quartzy bottle of inlet mouth air pump, escape pipe is bled from quartzy bottle by the air outlet air pump.
Air outlet as above air pump is communicated with nitrogengas cylinder by the gas circulation valve switch, and described inlet mouth air pump is communicated with nitrogengas cylinder.
Stopple coupon as above extend to quartzy bottle body bottom one end apart from quartzy bottle the bottle end length be 1/3 ~ 2/3 of quartzy bottle bottle height.
The utility model compared with prior art has the following advantages and effect:
1, open up new research field--biological metabolism photoresponse research, and the scientific research apparatus of novel high-efficient simple is provided for this reason;
2, simple in structure, easy to operate, can carry out simultaneously a plurality of processing and a plurality of repetition, greatly improve research speed and Efficiency;
3, stir the concussion of replacement shaking table with air lift type and stir, aeration condition is consistent, greatly reduces experimental error;
4, of many uses, can carry out the more novel fermentation factor and control, both can be used for the foster anaerobism that also can be used for of aerobic air culture and cultivated, also can be used for gas composition and control cultivation;
5, the detection of can taking a sample at any time, research cell response rule is more convenient.
Description of drawings
Fig. 1 is the structure iron of the first embodiment of the utility model device;
Fig. 2 is the structure iron of the second embodiment of the utility model device;
Fig. 3 is the structure iron of the third embodiment of the utility model device;
Fig. 4 is the structure iron of the 4th kind of embodiment of the utility model device;
In figure: the quartzy bottle of 1-; The 2-bottle cap; The 2-1-inlet pipe; The 2-2-escape pipe; 3-inlet mouth the first strainer; 3-1-inlet mouth the second strainer; 4-air outlet strainer; The 5-ventpipe; 6-air dispersion sieve; 7-inlet mouth air pump; 8-air outlet air pump; The 9-stopple coupon, 9-1-thief hole valve, 10-nitrogengas cylinder; 11-gas circulation valve switch; 12-pneumatic outlet valve switch.
Embodiment
Below in conjunction with accompanying drawing, the utility model is described in further detail:
Implement 1:
as shown in Figure 1, the biochemical culture apparatus of a kind of air lift type, comprise quartzy bottle 1, also comprise the bottle cap 2 that is arranged on quartzy bottle 1, be provided with inlet pipe 2-1 on bottle cap 2, escape pipe 2-2 and stopple coupon 9, the end of inlet pipe 2-1 extends to quartzy bottle 1 bottom and is provided with air dispersion sieve 6, the inlet pipe 2-1 the other end is connected strainer 3-1 by inlet mouth the first strainer 3 successively and is connected with inlet mouth air pump 7 with inlet mouth, one end of stopple coupon 9 extends to quartzy bottle 1 bottom, escape pipe 2-2 is connected with air outlet air pump 8 by air outlet strainer 4, inlet pipe 2-1 is by the past interior air-blowing of quartzy bottle 1 of inlet mouth air pump 7, escape pipe 2-2 bleeds from quartzy bottle 1 by air outlet air pump 8.
Embodiment 2: the application example in aerobic cell photoresponse research.
As shown in Figure 2, after first according to conventional aseptic technique, quartzy bottle 1 being injected the aseptic culture medium that filters or substratum is carried out high-temperature sterilization, be seated inlet mouth the first strainer 3, inlet mouth the second strainer 3-1, air outlet strainer 4 on bottle cap 2 after access aerobic microbiological bacterial classification or cell strain under aseptic condition.Three (or a plurality of) install postvaccinal combining, public same inlet mouth air pump 7 and air outlet air pump 8, and the pipe of connection is changeable as required.
According to experiment purpose, postvaccinal quartzy bottle 1 being placed under the different wave length source air-blowing cultivates, extract culture samples by stopple coupon 9 at any time according to the experiment needs and carry out the biochemical indicator detection in culturing process, sampling method is: syringe needle is injected sampling nozzle, then suction makes and is in low-pressure state in stopple coupon, then open thief hole valve 9-1, culture in bottle will be drawn out of rapidly, then closes rapidly thief hole valve 9-1, takes a sample complete.
Embodiment 3: the application example in anaerobic cell photoresponse research.
As described in Figure 3, after first according to conventional aseptic technique, quartzy bottle 1 being injected the aseptic culture medium that filters or substratum is carried out high-temperature sterilization, be seated inlet mouth the first strainer 3, inlet mouth the second strainer 3-1, air outlet strainer 4 on bottle cap 2 after access aerobic microbiological bacterial classification or cell strain under aseptic condition.Three (or a plurality of) install postvaccinal combining, and share same inlet mouth air pump 7 and air outlet air pump 8, and the pipe of connection is changeable as required.Air outlet air pump 8 is communicated with nitrogengas cylinder 10 by gas circulation valve switch 11, and described inlet mouth air pump 7 is communicated with nitrogengas cylinder 10.
According to experiment purpose, postvaccinal quartzy bottle 1 being placed under the different wave length source air-blowing cultivates, extract culture samples by stopple coupon 9 at any time according to the experiment needs and carry out the biochemical indicator detection in culturing process, sampling method is: syringe needle is injected sampling nozzle, then suction makes and is in low-pressure state in stopple coupon, then open thief hole valve 9-1, culture in bottle will be drawn out of rapidly, then closes rapidly thief hole valve 9-1, takes a sample complete.
Embodiment 4: the application example in heterotrophic cell and the research of autotrophic cell syntaxial system.
As shown in Figure 4, the application's device number is 2, share same inlet mouth air pump 7 and air outlet air pump 8, inlet mouth air pump 7 respectively with the inlet pipe 2-1 of a device be connected the escape pipe 2-2 of device and be connected, air outlet air pump 8 respectively with the escape pipe 2-2 of a device be connected the inlet pipe 2-1 of device and be connected.
As described in Figure 4, after first according to conventional aseptic technique, quartzy bottle 1 being injected the aseptic culture medium that filters or substratum is carried out high-temperature sterilization, under aseptic condition, one of them quartzy bottle 1 access heterotrophic microorganism bacterial classification is seated the sterilizing filter on bottle cap 2 after another quartzy bottle 1 inoculation photosynthetic cells strain.Postvaccinal quartzy bottle 1 gets up to form a little ecosystem that is hedged off from the outer world according to connection shown in Figure 4.
Then open inlet mouth air pump 7 and air outlet air pump 8, beginning air lift type stir culture.The carbonic acid gas that heterotrophic cell's respiration in process of growth discharges enters in recycle gas for autotrophic cell photosynthesis, autotrophic cell during releasing oxygen enters recycle gas in the photosynthesis process for heterotrophic cell's respiration.
Also can quartzy bottle 1 be placed in the adjusting effect of research optical source wavelength to autotrophic cell and heterotrophic cell's syntaxial system under the different wave length source according to experiment purpose.
Specific embodiment described herein is only to the explanation for example of the utility model spirit.The utility model person of ordinary skill in the field can make various modifications or replenishes or adopt similar mode to substitute described specific embodiment, but can't depart from spirit of the present utility model or surmount the defined scope of appended claims.
Claims (3)
1. the biochemical culture apparatus of an air lift type, comprise quartzy bottle (1), it is characterized in that: also comprise the bottle cap (2) that is arranged on quartzy bottle (1), be provided with inlet pipe (2-1) on bottle cap (2), escape pipe (2-2) and stopple coupon (9), one end of inlet pipe (2-1) extends to quartzy bottle (1) bottom and is provided with air dispersion sieve (6), inlet pipe (2-1) the other end is connected strainer (3-1) by inlet mouth the first strainer (3) successively and is connected with inlet mouth air pump (7) with inlet mouth, one end of stopple coupon (9) extends to bottom quartzy bottle (1), escape pipe (2-2) is connected with air outlet air pump (8) by air outlet strainer (4), inlet pipe (2-1) is by the past interior air-blowing of quartzy bottle (1) of inlet mouth air pump (7), escape pipe (2-2) is bled from quartzy bottle (1) by air outlet air pump (8).
2. the biochemical culture apparatus of a kind of air lift type according to claim 1, it is characterized in that: described air outlet air pump (8) is communicated with nitrogengas cylinder (10) by gas circulation valve switch (11), and described inlet mouth air pump (7) is communicated with nitrogengas cylinder (10).
3. the biochemical culture apparatus of a kind of air lift type according to claim 1 is characterized in that: it is 1/3 ~ 2/3 of quartzy bottle (1) bottle height apart from the length at the bottle end of quartzy bottle (1) that described stopple coupon (9) extends to quartzy bottle (1) bottom one end.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201220660135 CN202968563U (en) | 2012-12-04 | 2012-12-04 | Airlift biochemical culture device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201220660135 CN202968563U (en) | 2012-12-04 | 2012-12-04 | Airlift biochemical culture device |
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| Publication Number | Publication Date |
|---|---|
| CN202968563U true CN202968563U (en) | 2013-06-05 |
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| CN 201220660135 Expired - Fee Related CN202968563U (en) | 2012-12-04 | 2012-12-04 | Airlift biochemical culture device |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103045475A (en) * | 2012-12-04 | 2013-04-17 | 湖北民族学院 | Air-lift biochemical culture apparatus |
| CN103966092A (en) * | 2014-04-10 | 2014-08-06 | 欧东波 | Organ decellularization and circular fusion regeneration culture bottle |
| CN106190780A (en) * | 2016-08-31 | 2016-12-07 | 江苏绿扬生物电子工程有限公司 | A kind of layering gas-lifting type anaerobic biological reactor |
| CN106244440A (en) * | 2016-08-31 | 2016-12-21 | 江苏绿扬生物电子工程有限公司 | A kind of spiral gas-lifting type anaerobic biological reactor |
| CN106367328A (en) * | 2016-08-31 | 2017-02-01 | 江苏绿扬生物电子工程有限公司 | Air-lift type anaerobic bioreactor |
| CN112592824A (en) * | 2020-10-21 | 2021-04-02 | 英诺维尔智能科技（苏州）有限公司 | Reaction bottle for cell culture |
-
2012
- 2012-12-04 CN CN 201220660135 patent/CN202968563U/en not_active Expired - Fee Related
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103045475A (en) * | 2012-12-04 | 2013-04-17 | 湖北民族学院 | Air-lift biochemical culture apparatus |
| CN103045475B (en) * | 2012-12-04 | 2014-08-27 | 湖北民族学院 | Air-lift biochemical culture apparatus |
| CN103966092A (en) * | 2014-04-10 | 2014-08-06 | 欧东波 | Organ decellularization and circular fusion regeneration culture bottle |
| CN106190780A (en) * | 2016-08-31 | 2016-12-07 | 江苏绿扬生物电子工程有限公司 | A kind of layering gas-lifting type anaerobic biological reactor |
| CN106244440A (en) * | 2016-08-31 | 2016-12-21 | 江苏绿扬生物电子工程有限公司 | A kind of spiral gas-lifting type anaerobic biological reactor |
| CN106367328A (en) * | 2016-08-31 | 2017-02-01 | 江苏绿扬生物电子工程有限公司 | Air-lift type anaerobic bioreactor |
| CN112592824A (en) * | 2020-10-21 | 2021-04-02 | 英诺维尔智能科技（苏州）有限公司 | Reaction bottle for cell culture |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20130605 Termination date: 20141204 |
|
| EXPY | Termination of patent right or utility model |