CN101245310B - Series connected type unit cell algae optical biological reaction device - Google Patents
Series connected type unit cell algae optical biological reaction device Download PDFInfo
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- CN101245310B CN101245310B CN2008100106867A CN200810010686A CN101245310B CN 101245310 B CN101245310 B CN 101245310B CN 2008100106867 A CN2008100106867 A CN 2008100106867A CN 200810010686 A CN200810010686 A CN 200810010686A CN 101245310 B CN101245310 B CN 101245310B
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/58—Reaction vessels connected in series or in parallel
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/02—Photobioreactors
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/48—Holding appliances; Racks; Supports
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Abstract
The invention relates to a single cell algae cultivation device and provides a series connection type single cell algae photobiological reaction device to increase the production of the single cell algae and prolong the disinfection interval time of the device, the key points of the device are that: the device is formed a plurality of photobiological reaction units which are connected in series and have the same structure, each unit is provided with a water drain outlet, a sewage drain outlet and a base of an air inlet pipe, the edge of an opening at the lower end of a light transparent thin film cultivation cylinder is fixed by a lower fixed sleeve and the base which are connected by a bolt, the edge of the opening at the upper end of the light transparent thin film cultivation cylinder is fixed by an upper fixed sleeve and a top cover with water feed inlets which are connected by the bolts, a grid type shaping cover is inserted in the inner wall of the upper fixed sleeve and the lower fixed sleeve, the lower parts of the units are fixed on a support by the base, the upper parts of the units are suspended on a top frame by a pipe part and a connecting part which are connected with the water feed inlets, all the units are connected in series correspondingly by the own water feed inlet and the water drain outlet, a supply box which is arranged at the upper part of the top frame supplies an algal species and a culture liquid by a flowmeter, and the water feed inlet of the last unit is connected with a single cell algae collection box.
Description
Technical field
The present invention relates to the cultivation equipment of a kind of algae.
Background technology
The applicant applies for that on May 30th, 2005 its publication number is CN1699541A, and name is called a kind of patent application of single-cell algae optical bioreactor, and the disclosed technical characterictic of its technical scheme is to have the intake-outlet base, inserts the conduit on its water-in top.Insert the permanent sleeve in the base outer wall Z type seam, the edge of opening of light transmission film breed tube is positioned at the junction of base and permanent sleeve, and compress by being positioned at the bolt of base and the permanent sleeve outside on the boss, insert in the seam of permanent sleeve the bottom of graticule mesh formula typing cover, constituted the optical-biological reaction unit by above-mentioned part, it is to connect to form a cultivating system by intake-outlet separately is interlaced.But it has following shortcoming: because this system unitary water-in of top optical-biological reaction obtains is culturing liquid and water, after the each supply of system and gathering simultaneously, algae liquid concentration in each unit will be descended, and algae liquid concentration is by increasing in gradient behind the forward direction forever in each unit, owing to breed with the original algae kind that feeds all the time in each unit, its consequence is that the algae kind produces degeneration, and output also will descend; Contained harmful animal thing has long-term life and the condition of breeding in each unit in simultaneously each feeding, so must shorten system's sterilization and change the cycle that light transmission film is cultured bag.
Summary of the invention
The objective of the invention is to provides a kind of series connected type unit cell algae optical biological reaction device for the shortcoming that overcomes prior art, improves turnout, extension fixture disinfectant pitch time.
Technical solution of the present invention is as follows:
A kind of series connected type unit cell algae optical biological reaction device, comprise the base that its bottom is provided with down the mouth of a river and has the sewage draining exit of stopping valve, insert the following permanent sleeve in the Z type seam of base top, the edge of opening of light transmission film breed tube is positioned at the Z type seam junction of base and following permanent sleeve, and compress by the bolt on the boss that is positioned at base and following permanent sleeve outer side, insert in the following permanent sleeve female end bottom of graticule mesh formula typing cover, its feature also is: the top cover that also is provided with the band filling pipe end, its filling pipe end links to each other with teesaddle joint, last permanent sleeve in the Z type seam of insertion top cover bottom, the upper end that light transmission film is cultured tube also is an opening, edge of opening is positioned at the Z type seam junction of top cover and last permanent sleeve, and compress by the bolt on the boss that is positioned at top cover and last permanent sleeve outer side, the upper end of graticule mesh formula typing cover is inserted and is gone up in the permanent sleeve female end, described base also is provided with inlet pipe, and the inlet pipe end that is positioned at base top is provided with gas exhausting stone; A plurality of optical-biological reaction unit of forming by above-mentioned part, the top cover on its top is hung on the upper frame by web member, its underpart is fixed on the support by base separately, and be connected in series mutually by the mouth of a river up and down separately, the unitary filling pipe end of its first optical-biological reaction is connected with the cassette for supplying that algae kind and nutrient solution are housed on being located at upper frame by under meter, and the unitary filling pipe end of last optical-biological reaction links to each other with unit cell algae collection box.
Described a kind of series connected type unit cell algae optical biological reaction device, it is characterized in that described graticule mesh formula typing cover only is located at the unitary bottom of optical-biological reaction one segment distance, be positioned on the outside surface of light transmission film tube on graticule mesh formula typing cover top and be provided with anti-tensioner ring every a segment distance, more upwards, its spacing is big more.
Described a kind of series connected type unit cell algae optical biological reaction device is characterized in that on the Z type seam of described down permanent sleeve and last permanent sleeve the O RunddichtringO being housed.
From above-mentioned first solution as can be seen, because each optical-biological reaction unit serial connection that is the up/down perforation form, and last unitary filling pipe end is liquid outlet, so each inside, unit all is full of algae liquid; Be to be connected with the cassette for supplying that algae kind and nutrient solution are housed on upper frame because of first unitary filling pipe end again by under meter.According to cell capability, at every turn can two to four unit of can, these once by the unit group of can along with the form that each can all is stepping is pushed ahead, until being gathered.This has just overcome the shortcoming that prior art is bred by original dabbling algae kind all the time; Again because the algae liquid of being cultivated is that stepped form is pushed ahead, even contain harmful organisms in the algae liquid occasionally, can not jeopardize other can groups, also because the propelling form of this stepping, make and be difficult to the aggregation foreign material on the light transmission film wall, when the chance of the chance of harmful organisms survival and reproduction in the unit and aggregation foreign material reduces greatly, the disinfectant timed interval can prolong greatly in its system, the disinfectant program is simple simultaneously, can fill sterilized water to each unit from cassette for supplying, after keeping twenty four hours, emit sterilized water; In addition, because each unitary top cover all is hung on the upper frame by web member, unitary height is increased greatly, is 220 millimeters unit as same diameter, and the technical program can make cell height reach 1800 millimeters, and the top, unit of prior art can not set, there is stable problem, can only be less than 1500 millimeters, its output can increase by 20% in other words, simplified schedule of operation and labour intensity simultaneously, 30 times repetitive operations have been reduced to once.In addition, it can also be seen that from described solution: owing to inlet pipe is housed in each unit base, can constantly import the new air that contains carbonic acid gas during breed, to replenish unit cell algae light and to make needed carbonic acid gas of time spent, this is highly beneficial to the growth that promotes the unit cell algae, the effect of its gas exhausting stone is to make the air distribution that enters in the breed unit even, certainly its mixing effect is also better, can fully take away the oxygen that produces owing to photosynthesis attached to the unit cell algae, above-mentioned effect is even more important to this very big breed unit of algae liquid density, device rear end.
Description of drawings
Fig. 1 is the structural representation of a solution of the present invention.
1 is base among the figure, and 2 are the following mouth of a river, and 3 are permanent sleeve down, and 4 culture tin for light transmission film, 5 is support, and 6 are graticule mesh formula typing cover, and 7 is filling pipe end, 8 is top cover, and 9 is last permanent sleeve, and 11 is clamping flange, 12 is upper frame, and 13 is inlet pipe, and 14 is gas exhausting stone, 15 is vapor pipe, and 16 is cassette for supplying, and 17 are anti-packing, 18 is the O RunddichtringO, and 19 is the dewatering outlet, and 20 is under meter.
Embodiment
Now will be in conjunction with the embodiments (accompanying drawing) the invention will be further described:
As shown in Figure 1, its bottom is provided with down the mouth of a river 2 and has the base 1 of the sewage draining exit 19 of stopping valve, insert the following permanent sleeve 3 that has O RunddichtringO 18 in the Z type seam of base top, the edge of opening of light transmission film breed tube 4 is positioned at the Z type seam junction of base and following permanent sleeve, and compress by the bolt on the boss that is positioned at base and following permanent sleeve outer side, insert in the following permanent sleeve female end bottom of graticule mesh formula typing cover 6, the length of graticule mesh formula typing cover is 1/3rd of light transmission film breed tube length degree, on the printing opacity breed tube on graticule mesh formula typing cover top, be provided with anti-tensioner ring 17 every a segment distance, more upwards, its spacing is big more, its objective is in order to increase light transmission film to culture the illumination of tube; The top cover 8 of band filling pipe end 7, its filling pipe end links to each other with teesaddle joint, the last permanent sleeve 9 that has O RunddichtringO 18 in the Z type seam of insertion top cover bottom, the upper end that light transmission film is cultured tube also is an opening, its edge of opening is positioned at the Z type seam junction of top cover and last permanent sleeve, and compress by the bolt on the boss that is positioned at top cover and last permanent sleeve outer side, described base 1 also is provided with inlet pipe 13, gas exhausting stone 14 is equipped with in the inlet pipe end that is positioned at base top, 30 optical-biological reaction unit of forming by above-mentioned part, top, unit is hung on the upper frame on 12 by the intubate 15 usefulness flange clamps 11 on the clear opening of the teesaddle joint that links to each other with top cover, also available four Clamping Apparatus of Connecting Rod replace flange clamp, the bottom, unit is fixed on the support 5 by base separately, each unit mouth of a river up and down by separately again is connected in series mutually, its first unitary filling pipe end is connected with the cassette for supplying 16 that algae kind and nutrient solution are housed on being located at upper frame 12 by under meter 20, and last unitary filling pipe end links to each other with unit cell algae collection box.Present embodiment is work like this: close the stopping valve on each unit sewage draining exit, fill with through the disinfectant clear water to all unit, open under meter, annotate the nutrient solution that contain algae kind by cassette for supplying to first three unit by filling pipe end, until filling.Close under meter 20, in the unit, supply through the disinfectant air by inlet pipe 13, it passes the unit and upwards discharges by vapor pipe 15, through twenty four hours, fill until Unit the 4th to the 6th by the nutrient solution that contains the algae kind of three cell capabilities of under meter perfusion again, close under meter, for the disinfectant air, this moment, Unit first to the 3rd was new dabbling algae kind and nutrient solution to the unit that algae liquid is arranged.Unit the 4th to the 6th is first day dabbling algae kind and nutrient solution, until the 11 day, when under meter pours into identical flow again, from every milliliter of algae liquid that contains 1.5 hundred million unit cell algaes of three cell capabilities of the 30 unitary filling pipe end discharge, algae kind content is every milliliter of 1,000 ten thousand unit cell algaes in its cassette for supplying.In the time of will sterilizing to this device, open each unitary sewage draining exit, behind the emptying algae liquid, close sewage draining exit, by under meter, fill thimerosal by cassette for supplying to whole unit again, through twenty four hours, the emptying thimerosal reactivates this device.
Claims (3)
1. series connected type unit cell algae optical biological reaction device, comprise the base (1) that its bottom is provided with down the mouth of a river (2) and has the sewage draining exit (19) of stopping valve, insert the following permanent sleeve (3) in the Z type seam of base top, the edge of opening of light transmission film breed tube (4) is positioned at the Z type seam junction of base and following permanent sleeve, and compress by the bolt on the boss that is positioned at base and following permanent sleeve outer side, insert in the following permanent sleeve female end bottom of graticule mesh formula typing cover (6), its feature also is: the top cover (8) that also is provided with band filling pipe end (7), its filling pipe end links to each other with teesaddle joint, last permanent sleeve (9) in the Z type seam of insertion top cover bottom, the upper end that light transmission film is cultured tube also is an opening, edge of opening is positioned at the Z type seam junction of top cover and last permanent sleeve, and compress by the bolt on the boss that is positioned at top cover and last permanent sleeve outer side, the upper end of graticule mesh formula typing cover (6) is inserted and is gone up in the permanent sleeve female end, described base (1) also is provided with inlet pipe (13), and the inlet pipe end that is positioned at base top is provided with gas exhausting stone (14); A plurality of optical-biological reaction unit of forming by above-mentioned part, the top cover on its top is hung on the upper frame (12) by web member, its underpart is fixed on the support (5) by base separately, and be connected in series mutually by the mouth of a river up and down separately, the unitary filling pipe end of its first optical-biological reaction is connected with the cassette for supplying (16) that algae kind and nutrient solution are housed on being located at upper frame (12) by under meter, and the unitary filling pipe end of last optical-biological reaction links to each other with unit cell algae collection box.
2. a kind of series connected type unit cell algae optical biological reaction device according to claim 1, it is characterized in that described graticule mesh formula typing cover (6) only is located at the unitary bottom of optical-biological reaction one segment distance, be positioned on the outside surface of light transmission film tube on graticule mesh formula typing cover top and be provided with anti-tensioner ring (17) every a segment distance, more upwards, its spacing is big more.
3. a kind of series connected type unit cell algae optical biological reaction device according to claim 1 and 2 is characterized in that on the Z type seam of described down permanent sleeve (3) and last permanent sleeve (9) O RunddichtringO (18) being housed.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2008100106867A CN101245310B (en) | 2008-03-17 | 2008-03-17 | Series connected type unit cell algae optical biological reaction device |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2008100106867A CN101245310B (en) | 2008-03-17 | 2008-03-17 | Series connected type unit cell algae optical biological reaction device |
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| CN101245310A CN101245310A (en) | 2008-08-20 |
| CN101245310B true CN101245310B (en) | 2011-07-20 |
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| CN2008100106867A Active CN101245310B (en) | 2008-03-17 | 2008-03-17 | Series connected type unit cell algae optical biological reaction device |
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Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101422129B (en) * | 2008-12-02 | 2011-05-11 | 大连汇新钛设备开发有限公司 | Macroscopic algae seedling indoor culturing method and device |
| WO2011007250A2 (en) * | 2009-07-17 | 2011-01-20 | M2M Engineering S.A.S. | Industrial photobioreactor and structure of the same, of low cost and with high productive yield for occupied surface site |
| CN102660450A (en) * | 2012-05-04 | 2012-09-12 | 逢甲大学 | Alga culturing device and combination thereof |
| CN104928146B (en) * | 2014-03-17 | 2017-07-11 | 孙伟春 | Closed open dual-purpose type self-loopa filtering aeration algae cultivation module |
| CN105349422A (en) * | 2015-12-03 | 2016-02-24 | 遵义医学院 | Plant cell suspension culture device |
| WO2022108437A1 (en) * | 2020-11-23 | 2022-05-27 | Echevarria Parres Antonio Jose De Jesus De San Juan Bosco | Photobioreactor for growing microalgae |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2570287Y (en) * | 2002-09-20 | 2003-09-03 | 栾龙 | Plant cell large scale culture biological reaction equipment |
| CN1699541A (en) * | 2005-05-30 | 2005-11-23 | 孙建明 | Single-cell algae optical bioreactor |
| CN1721523A (en) * | 2004-07-16 | 2006-01-18 | 中国科学院海洋研究所 | The pipeline bioreactor that microalgae mass is cultivated |
| CN1966660A (en) * | 2006-11-20 | 2007-05-23 | 宁波大学 | Device and method for large scale culturing Haemotococcum pluvies and converting astaxanthin |
| CN201214660Y (en) * | 2008-04-16 | 2009-04-01 | 大连汇新钛设备开发有限公司 | Algae culture apparatus |
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- 2008-03-17 CN CN2008100106867A patent/CN101245310B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2570287Y (en) * | 2002-09-20 | 2003-09-03 | 栾龙 | Plant cell large scale culture biological reaction equipment |
| CN1721523A (en) * | 2004-07-16 | 2006-01-18 | 中国科学院海洋研究所 | The pipeline bioreactor that microalgae mass is cultivated |
| CN1699541A (en) * | 2005-05-30 | 2005-11-23 | 孙建明 | Single-cell algae optical bioreactor |
| CN1966660A (en) * | 2006-11-20 | 2007-05-23 | 宁波大学 | Device and method for large scale culturing Haemotococcum pluvies and converting astaxanthin |
| CN201214660Y (en) * | 2008-04-16 | 2009-04-01 | 大连汇新钛设备开发有限公司 | Algae culture apparatus |
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Denomination of invention: A tandem monocytogenes photobioreaction device Effective date of registration: 20220803 Granted publication date: 20110720 Pledgee: Industrial and Commercial Bank of China Limited Dalian high tech Park sub branch Pledgor: DALIAN HUIXIN TITANIUM EQUIPMENT DEVELOPMENT Co.,Ltd. Registration number: Y2022980011869 |