CN201915083U - Efficient culturing device for photosynthetic bacteria - Google Patents

Efficient culturing device for photosynthetic bacteria Download PDF

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Publication number
CN201915083U
CN201915083U CN 201020536204 CN201020536204U CN201915083U CN 201915083 U CN201915083 U CN 201915083U CN 201020536204 CN201020536204 CN 201020536204 CN 201020536204 U CN201020536204 U CN 201020536204U CN 201915083 U CN201915083 U CN 201915083U
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Prior art keywords
support
light source
photosynthetic bacterium
culture device
efficient
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Expired - Fee Related
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CN 201020536204
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Chinese (zh)
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朱益明
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JIANGSU BANGDE BIOLOGICAL TECHNOLOGY DEVELOPMENT Co Ltd
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JIANGSU BANGDE BIOLOGICAL TECHNOLOGY DEVELOPMENT Co Ltd
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Priority to CN 201020536204 priority Critical patent/CN201915083U/en
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00Bioreactors or fermenters specially adapted for specific uses
    • C12M21/02Photobioreactors
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M31/00Means for providing, directing, scattering or concentrating light
    • C12M31/10Means for providing, directing, scattering or concentrating light by light emitting elements located inside the reactor, e.g. LED or OLED
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M39/00Means for cleaning the apparatus or avoiding unwanted deposits of microorganisms

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  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The utility model relates to an efficient culturing device for photosynthetic bacteria, which comprises a hollow cylinder culture vessel which is internally provided with a light source and a protecting cover, the culture vessel and the light source protecting cover are made from acrylic or glass. An upper end cover of the vessel is provided with a drive motor, an agitator support, a cleaning brush, a drive control system and an air cleaner; the lower end of the vessel is provided with an underpinning plate, a support, an anti-corrosion pipeline, a control valve and a water pump, wherein the anti-corrosion pipeline is used for material fluid feeding and discharging, the upper end cover, the underpinning plate and the support are made of 304 stainless steel, and the pipeline, the control valve and the water pump are made at a corrosion-proof level. The efficient culturing device for the photosynthetic bacteria has the advantages that a reasonable light surface and volume ratio is designed according to the pierce degree of the light, the efficient culturing device is internally provided with an effective light source, the nature light source is fully used by the effective light source combined with the transparent vessel, thereby improving the light sources utilization, the control system timing controls the agitator support brush to efficiently prevent the adherence phenomenon caused by the bacteria focusing light, the culturing device with the air cleaner ensures the anaerobic condition of the culture liquid in the agitating process, efficiently prevents the pollution of the outside miscellaneous bacteria and improves the bacterium fluid purity. The efficient culturing device for the photosynthetic bacteria is concise and convenient to install and use, has low cost, can realize the high standardized and efficient scale production, and is suitable for enterprises of various scales to use.

Description

The high-efficient culture device of a kind of photosynthetic bacterium
Affiliated technical field
The culture apparatus that the utility model relates to microbial preparation is the culture apparatus of photosynthetic bacterium especially, specially refers to a kind ofly to contain timing control system, built-in light source, stir, clean automatically efficient, practical, the culture apparatus cheaply of the adherent photosynthetic bacterium of bacterium automatically.
Background technology
Light is a kind of under different physical environments with bacterium, has multiple heterotrophism function.Have a wide range of applications in fields such as aquaculture, poultry industry, plant husbandry, purification of water quality and new energy developments.
At present, the training method of photosynthetic bacterium have the totally enclosed anaerobism cultivate and the opening air culture that declines foster.The totally enclosed cultivation generally is used for the laboratory more cultivates on a small scale, and open type (cement pit) cultivation illumination condition is bad, and bacterium has the adherent phenomenon of optically focused, also needs manually to stir, and the living contaminants degree is big in the culturing process, poor growth.It is low to cultivate the cell density that can reach, and fails easily, usually can not continuous production, labour intensity is big, is not suitable for the large-scale production of modern high standard microbial bacteria.
Summary of the invention
In order to overcome above-mentioned the deficiencies in the prior art, the utility model provides the high-efficient culture device of a kind of photosynthetic bacterium, has improved the efficiency of light energy utilization effectively.It is big to have solved adherent phenomenon of bacterium optically focused and labour intensity, has reduced the living contaminants rate, and quality product and output are significantly improved, and is fit to the quick and large-scale production of photosynthetic bacterium.
The technical scheme that the utility model adopted is: the high-efficient culture device of a kind of photosynthetic bacterium comprises transparent hollow circular cylinder culture vessel, described hollow culture vessel is meant and adopts diameter that transparent acrylic or glass makes at 50-70 centimetre that height is generally 1.5-2.5 rice.Container built-in light source and sheath, light source adopts the light of 400-700nm, and described light source sheath adopts acrylic or glass to make.Container upper end lid, driving motor, stirring support and cleaning brush and driving control system, described upper end cover, stirring support adopt 304 stainless materials to make, described cleaning brush adopts rubber or mane to make, and described transmission system comprises power switch, time timing controller, rotating triggering device and wire etc.Be provided with air scrubber on upper end cover, described air scrubber is made of 304 stainless materials, establishes in the filter with multi-layer air filter paper and cavernous body, is sprayed with sterilant on cavernous body.Empty filter-identical with hollow light source sheath can be given the heat radiation of light source sheath and be got rid of the gas that thalline produces.The container lower end is provided with anti-corrosion pipeline, control valve and the water pump of butt plate, support, turnover feed liquid, and described butt plate, support adopt 304 stainless materials to make, and described pipeline, control valve, water pump adopt anticorrosion grade.
Compared with prior art the utility model advantage is:
1, the closed culture apparatus that has the exhaust heat sinking function has guaranteed the anaerobic condition of cultivation liquid in the whipping process, has prevented the pollution to institute cultured microorganism bacterium of extraneous assorted bacterium and harmful animal effectively, can effectively improve bacterium liquid purity.
2, built-in efficient light sources can make full use of lamp, saved cost and shorten incubation time in conjunction with transparent container, has improved economic benefit.
3, according to the degree of penetrating of light, designed the long-pending and volume ratio of rational optical surface, make the light source utilization ratio higher, ensured that the competent light source of bacterium high-density growth and rational disposable output improve.
4, the stirring support brush by timing control system control can prevent timely and effectively that, thalline low because of the adherent transmittance that causes of bacterium optically focused is subjected to phenomenons such as light is inhomogeneous, thalline sinks to the bottom, and makes bacterium liquid thalline fully, evenly be subjected to photoproduction long.
5, the installation of the utility model device total system and convenience ease in use, cost is low, can realize high standardization, efficient mass-producing cultivation biological bacteria, is fit to the use of each scale enterprise.
Description of drawings
Fig. 1: the positive structure sectional view of the utility model embodiment
Embodiment
With reference to the accompanying drawings the utility model is described further below.
The high-efficient culture device of a kind of photosynthetic bacterium shown in Fig. 1 be diameter 50-70 centimetre of making by transparent acrylic or glass, high 1.5-2.5 rice hollow cylindrical container 1., 3. container 1. upper end has one the 304 stainless arc lid of material, and limit that the lower end turnover of arc lid is 1-2 centimetre and container be tight closure (it is bonding to strengthen that glass cement is coated in the junction) 1..Have the hole at arc lid middle position 3., be used to install driving motor 9. with the bearing connection of support 10..Stir support and 6. 9. adopt bearing connection 10. closely to be connected with motor, 6. support adopts two bar balances, three rod-types to design, support be equipped with on 6. be used for the clean container wall 1. with light source sheath brush 5. 4..Described support adopts 304 stainless steel material to make, and described light source sheath adopts acrylic or glass to make, and described brush adopts rubber or mane to make, and 3. described light source sheath is connected with the arc lid and 8. communicates with air scrubber.8. described air scrubber adopts 304 material stainless steels to make, at 8. built-in multi-layer air filter paper of air scrubber and cavernous body and be sprayed with sterilant.3. go up at arc lid, an air scrubber side 8. is equipped with control enclosure (11), and trip switchs such as built-in light source, driving motor, timer, wire etc. are housed in the control enclosure.Also the control device of organizing culture vessel can be together in series more is contained in the controlling box, functions such as the auto-switch light source of the incubators of group more than can realizing, regularly stirring, cleaning.1. the arc butt plate is housed 2. in the lower end at container, 2. described arc butt plate is to adopt 304 material stainless steels to make, and has the lid of upper overturning, with 1. tight closure (coat glass cement once bonding) by force of container.Have diameter 3-5 centimetre hole and the pipe connecting piece (12) that 304 material stainless steels are made is housed at arc butt plate middle position 2., the control valve (13) of turnover feed liquid is housed between pipe connecting piece (12) and pipeline (15), end at pipeline is equipped with water pump (14), and described control valve (13), pipeline (15) and water pump (14) all adopt antifouling level.The arc butt plate 2. below the fixed support support body of form as culture vessel such as be welded with A-frame (16) or adopt that fragment of brick is built by laying bricks or stones.

Claims (9)

1. the high-efficient culture device of a photosynthetic bacterium, it is characterized in that: the high-efficient culture device of a kind of photosynthetic bacterium is by clear hollow cylindrical vessel (1), there is arc lid (3) container (1) upper end, middle position at arc lid (3) has the hole, is used to install the bearing connection (10) of driving motor (9) and support; Support (6) adopts bearing connection (10) closely to be connected with motor (9), and support (6) adopts two bar balances, three a rod-types design, and the brush (4) that is used for clean container wall (1) and light source sheath (5) is housed on support (6); One side last at arc lid (3), air scrubber (8) is equipped with control enclosure (11), and built-in light source, driving motor, trip switch, timer, wire are housed in the control enclosure; In container (1) lower end arc butt plate (2) is housed, middle position at arc butt plate (2) has the hole and adorns the pipe connecting piece (12) that stainless steel is made, the control valve (13) of turnover feed liquid is housed between pipe connecting piece (12) and pipeline (15), end at pipeline is equipped with water pump (14), and described control valve (13), pipeline (15) and water pump (14) all adopt antifouling level; Be welded with A-frame (16) in the below of arc butt plate (2) or adopt fragment of brick to build the fixed support support body of form by laying bricks or stones as culture vessel.
2. according to the high-efficient culture device of the described a kind of photosynthetic bacterium of claim 1, it is characterized in that: hollow cylindrical container (1), light source sheath are made by transparent acrylic or glass.
3. the high-efficient culture device of a kind of photosynthetic bacterium according to claim 1 is characterized in that: the diameter of hollow cylindrical container (1) adopts 50-70 centimetre, and height is designed to the ratio of 1.5-2.5 rice.
4. the high-efficient culture device of a kind of photosynthetic bacterium according to claim 1, it is characterized in that: the built-in stirring support of culture vessel (6) adopts bearing connection (10) closely to be connected with upper end motor (9), and the brush (4) that is used for clean container wall (1) and light source sheath (5) is housed on support (6).
5. the high-efficient culture device of a kind of photosynthetic bacterium according to claim 4 is characterized in that: being contained in the brush (4) that is used for clean container wall (1) and light source sheath (5) on the support (6) is to adopt rubber or mane to make.
6. the high-efficient culture device of a kind of photosynthetic bacterium according to claim 1 is characterized in that: the built-in light source sheath is connected with arc lid (3) and communicates with air scrubber (8).
7. the high-efficient culture device of a kind of photosynthetic bacterium according to claim 1 is characterized in that: built-in light source, stirring support, the control of cleaning brush employing timing control system.
8. the high-efficient culture device of a kind of photosynthetic bacterium according to claim 1 is characterized in that: arc lid (3), arc butt plate (2), bearing connection (10) and support (6), air scrubber (8) are to adopt 304 material stainless steels to make.
9. the high-efficient culture device of a kind of photosynthetic bacterium according to claim 7, it is characterized in that: Controlling System is made up of power switch, power supply protector, time timer, rotating triggering device and power lead.
CN 201020536204 2010-09-13 2010-09-13 Efficient culturing device for photosynthetic bacteria Expired - Fee Related CN201915083U (en)

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Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102973961A (en) * 2012-11-22 2013-03-20 华侨大学 Ozone sterilization method for photobioreactor for culturing photosynthetic bacteria in large scale
CN102994367A (en) * 2012-11-22 2013-03-27 华侨大学 High-efficiency phototroph reaction system for pure culture of photosynthetic bacteria and sterilizing method thereof
CN102994424A (en) * 2012-11-22 2013-03-27 华侨大学 Method for efficiently and continuously collecting super-concentrated photosynthetic bacteria thalli
CN103122306A (en) * 2011-11-21 2013-05-29 镇江日泰生物工程设备有限公司 Photosynthetic cultivation fermentation device
CN107488581A (en) * 2017-10-16 2017-12-19 江苏苏港和顺生物科技有限公司 A kind of photosynthetic bacteria production equipment
CN108410702A (en) * 2018-03-13 2018-08-17 尹康康 A kind of photosynthetic bacteria culture instrument
CN108485941A (en) * 2018-03-13 2018-09-04 尹康康 A kind of photosynthetic bacteria cultural method
CN109400241A (en) * 2019-01-09 2019-03-01 苏州寰宝农业科技有限公司 A kind of cultivation bucket and its use the method for preparing organic selenium fertilizer
CN112063519A (en) * 2020-09-14 2020-12-11 中国科学院重庆绿色智能技术研究院 Photosynthetic bacteria continuous culture device and control method thereof

Cited By (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103122306A (en) * 2011-11-21 2013-05-29 镇江日泰生物工程设备有限公司 Photosynthetic cultivation fermentation device
CN102973961A (en) * 2012-11-22 2013-03-20 华侨大学 Ozone sterilization method for photobioreactor for culturing photosynthetic bacteria in large scale
CN102994367A (en) * 2012-11-22 2013-03-27 华侨大学 High-efficiency phototroph reaction system for pure culture of photosynthetic bacteria and sterilizing method thereof
CN102994424A (en) * 2012-11-22 2013-03-27 华侨大学 Method for efficiently and continuously collecting super-concentrated photosynthetic bacteria thalli
CN102994424B (en) * 2012-11-22 2015-08-05 华侨大学 A kind of high-efficiency and continuous collects the method for hyperconcentration photosynthetic bacterial thallus
CN107488581A (en) * 2017-10-16 2017-12-19 江苏苏港和顺生物科技有限公司 A kind of photosynthetic bacteria production equipment
CN108410702A (en) * 2018-03-13 2018-08-17 尹康康 A kind of photosynthetic bacteria culture instrument
CN108485941A (en) * 2018-03-13 2018-09-04 尹康康 A kind of photosynthetic bacteria cultural method
CN108410702B (en) * 2018-03-13 2021-09-21 南京棠邑科创服务有限公司 Photosynthetic bacteria culture instrument
CN108485941B (en) * 2018-03-13 2021-09-21 山东安特博迪生物技术中心 Photosynthetic bacterium culture method
CN109400241A (en) * 2019-01-09 2019-03-01 苏州寰宝农业科技有限公司 A kind of cultivation bucket and its use the method for preparing organic selenium fertilizer
CN112063519A (en) * 2020-09-14 2020-12-11 中国科学院重庆绿色智能技术研究院 Photosynthetic bacteria continuous culture device and control method thereof
WO2022052418A1 (en) * 2020-09-14 2022-03-17 中国科学院重庆绿色智能技术研究院 Continuous culture apparatus for photosynthetic bacteria and control method therefor
CN112063519B (en) * 2020-09-14 2022-06-10 中国科学院重庆绿色智能技术研究院 Photosynthetic bacteria continuous culture device and control method thereof

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