WO2018086326A1

WO2018086326A1 - Photobioreactor

Info

Publication number: WO2018086326A1
Application number: PCT/CN2017/084480
Authority: WO
Inventors: 卢运明; 温永煌; 张碧松; 周安善; 马春苓; 熊伟; 艾咏平; 罗杰
Original assignee: 深圳市绿得宝保健食品有限公司
Priority date: 2016-11-11
Filing date: 2017-05-16
Publication date: 2018-05-17
Also published as: CN206204331U

Abstract

A photobioreactor, comprising a liquid storage tank, a photosynthetic reaction pipe provided at the top of the liquid storage tank and running through the liquid storage tank, an air pipe provided at the top of the photosynthetic reaction pipe, a vacuum pump and a solenoid valve in communication with the air pipe, a nano aeration pipe provided in the liquid storage tank and connected to an exhaust pipe of the vacuum pump, and an exhaust fan provided at the top of the liquid storage tank, and an air purification device for injecting clean air into the liquid storage tank. The photobioreactor further comprises a carbon dioxide supply device connected to the exhaust pipe of the vacuum pump and the nano aeration pipe, an online detection device provided in the liquid storage tank, and an automatic nutrient feeding device for nutrient supply.

Description

Photosynthetic bioreactor

Technical field

The present disclosure relates to the field of biotechnology, for example to a photosynthetic bioreactor.

Background technique

A photosynthetic bioreactor is a device for photosynthetic biological cell or tissue culture with high photosynthetic efficiency. For example, organisms such as microalgae and photosynthetic bacteria located in photosynthetic bioreactors are capable of high-density, high-yield, high-quality continuous and semi-continuous cultures. In addition, photosynthetic bioreactors can produce high protein, fat, biofuel, a variety of bioactive carbohydrates and many biochemical products with high added value. Therefore, photosynthetic bioreactors are concerned by researchers and companies at home and abroad. . However, the shortcomings of the complex structure and low production efficiency of the photosynthetic bioreactor need to be improved.

Summary of the invention

The present disclosure provides a photosynthetic bioreactor that is simple in structure and capable of conveniently and efficiently performing photosynthetic biological cell or tissue culture.

The present embodiment provides a photosynthetic bioreactor, which may include a liquid storage tank, a photosynthetic biological reaction tube disposed at the top of the liquid storage tank and disposed through the liquid storage tank, and disposed on the top of the photosynthetic biological reaction tube. a gas pipe, a vacuum pump and a solenoid valve connected to the gas pipe, a nano-aeration pipe disposed in the liquid storage tank connected to an exhaust pipe of the vacuum pump, an exhaust fan disposed at a top of the liquid storage tank, and An air purifying device for cleaning air into the reservoir.

Optionally, the photosynthetic bioreactor may further comprise a carbon dioxide supply device connected through the exhaust pipe or the nano-aeration tube of the vacuum pump, an on-line detecting device disposed in the liquid storage tank, and a nutrient for feeding The supplied nutrient is automatically added to the device.

Optionally, the vacuum pump, the exhaust fan, the air purifying device, the carbon dioxide supply device, the in-line detecting device, and the automatic nutrient adding device may be disposed in a liquid storage The electronic control unit outside the box is connected.

Optionally, the top of the photosynthetic biological reaction tube may be provided with a detachable top cover, and the vacuum pump is disposed above the top cover.

Alternatively, the photosynthetic biological reaction tube may be provided as a transparent glass tube or a transparent plastic tube.

Alternatively, the air cleaning device may be provided as an air cleaner.

Optionally, the photosynthetic bioreactor may further include a bracket for erecting the liquid storage tank and the photosynthetic biological reaction tube, and a bottom of the bracket is provided with a universal wheel.

Optionally, a steam separator may be disposed at a junction of the vacuum pump and the air pipe.

Alternatively, the photosynthetic biological reaction tube may be configured as a single tube or a plurality of tubes.

Optionally, the photosynthetic biological reaction tube has an outer diameter of 10 mm or more and 600 mm or less; and the photosynthetic biological reaction tube has a height of 20 mm or more and 95000 mm or less.

Alternatively, the photosynthetic biological reaction tube may be provided as an upright tube.

The embodiment provides a photosynthetic bioreactor capable of automatically moving up and down the culture liquid in the photosynthetic biological reaction tube under the adjustment of the vacuum pump and the electromagnetic valve, and also through the nano aeration tube, the exhaust fan and The function of the air purifying device is such that the culture liquid in the liquid storage tank is sufficiently stirred, thereby facilitating efficient and efficient photosynthetic biological cell or tissue culture.

DRAWINGS

FIG. 1 is a schematic structural view of a photosynthetic bioreactor provided by the embodiment.

2 is a schematic structural view of a photosynthetic biological reaction tube provided by the embodiment.

detailed description

The technical solutions of the present disclosure will be described below in conjunction with the accompanying drawings and by alternative embodiments. In the case of a conflict, the technical solutions in the following embodiments and embodiments can be combined with each other.

As shown in FIG. 1 , the present embodiment provides a photosynthetic bioreactor, for example, a Cavend photobioreactor, which may include a liquid storage tank 1 disposed on the top of the liquid storage tank 1 and The photosynthetic biological reaction tube 2 disposed through the liquid storage tank 1 is disposed on the top of the photosynthetic biological reaction tube 2 a gas pipe, a vacuum pump 3 connected to the gas pipe, a solenoid valve 32, a nano-aeration pipe 11 disposed in the liquid storage tank 1 and connected to an exhaust pipe of the vacuum pump 3, and a liquid aeration pipe 11 disposed in the liquid storage tank 1 The top exhaust fan and the air purifying device for cleaning the air into the reservoir 1 are provided.

Alternatively, when the vacuum pump 3 and the solenoid valve 32 communicating with the gas pipe at the top of the photosynthetic biological reaction tube 2 are in a closed state, the liquid storage tank 1 and the photosynthetic biological reaction tube 2 are in a sealed state, and the liquid storage tank 1 and the photosynthetic biological reaction The gas in the tube 2 cannot interact with the outside world. When the vacuum pump 3 is opened and the solenoid valve 32 is closed, the gas in the reservoir 1 and the photosynthetic bioreactor 2 is gradually removed, so that the photosynthetic bioreactor and the reservoir are in a negative pressure state. In order to balance the negative pressure state of the photosynthetic biological reaction tube and the liquid storage tank, the electromagnetic valve can be opened, and the external gas enters the photosynthetic biological reaction tube and the liquid storage tank, so that the air pressure in the photosynthetic biological reaction tube and the liquid storage tank is the same as the external atmospheric pressure.

In the photosynthetic bioreactor of the embodiment, when the culture liquid is filled in the liquid storage tank, the vacuum pump is turned on, and the electromagnetic valve is closed, so that the gas in the photosynthetic biological reaction tube is gradually discharged, and the photosynthetic biological reaction tube and the storage are further The sealed space formed by the liquid tank forms a certain negative pressure, and the culture liquid located in the liquid storage tank gradually rises in the height direction of the photosynthetic biological reaction tube. The electromagnetic valve 32 is opened, and the vacuum pump 2 is turned off, so that the photosynthetic biological reaction tube is filled with gas, and the air pressure in the sealed space formed by the photosynthetic biological reaction tube and the liquid storage tank is the same as the external atmospheric pressure, and the liquid level in the photosynthetic biological reaction tube Gradually falling. Through the cooperation of the vacuum pump and the electromagnetic valve, the air pressure in the closed space formed by the photosynthetic biological reaction tube and the liquid storage tank is constantly changed, and the change of the air pressure enables the culture liquid to move up and down along the height direction of the photosynthetic biological reaction tube, thereby effectively enhancing the photosynthesis. The contact of the culture solution in the biological reaction tube with light improves the efficiency of photosynthetic reaction.

In this embodiment, the method further includes: providing a carbon dioxide supply device 4 connected to the exhaust pipe 31 or the nano-aeration pipe 11 of the vacuum pump 3, an in-line detecting device disposed in the liquid storage tank 1, and Automatic feeding device for nutrients supplied by nutrients. The vacuum pump 3, the exhaust fan, the air purifying device, the carbon dioxide supply device 4, the in-line detecting device, and the automatic nutrient adding device are all electrically controllable outside the liquid storage tank 1. The devices are connected. The air purifying device in this embodiment may be provided as an air cleaner. With this structure design, clean air can be input into the liquid storage tank through the air filter, thereby generating rising bubbles in the photosynthetic biological reaction tube, and uniformly stirring the photosynthesis. The culture liquid in the biological reaction tube, and the generated gas enters the nano-aeration tube through the exhaust of the vacuum pump, thereby conveniently bubbling and stirring the culture liquid in the liquid storage tank, and the excess gas is set in the storage. The exhaust fan at the top of the tank is drained.

The photosynthetic bioreactor provided in this embodiment can automatically stop when the vacuum pump is operated for a pre-controlled time. After the vacuum pump stops working, the solenoid valve 32 is in an open state, and the atmosphere enters the photosynthetic biological reaction tube through the air filter, and the water level of the culture liquid begins to drop until it returns to the liquid storage tank. Under the alternate working conditions of the vacuum pump and the solenoid valve 32, the liquid in the photosynthetic biological reaction tube is repeatedly moved up and down. The organisms in the photosynthetic biological reaction tube receive light irradiation and perform high-efficiency photosynthesis in a state of being sufficiently mixed and uniformly. During this work, the culture solution is only in contact with the gas, the shearing force is small, and it is in a fully sealed, clean aquaculture environment, which is beneficial to the pure culture of the organism.

In addition, in the embodiment, a manual valve 33 can be connected in parallel with the gas pipe on the top of the photosynthetic biological reaction tube 2, so that the liquid level in the photosynthetic biological reaction tube can be conveniently and effectively adjusted.

In the present embodiment, the flow of the culture solution between the reservoir and the photosynthetic bioreactor can be achieved by vacuum negative pressure and automated control techniques.

In this embodiment, the top of the photosynthetic biological reaction tube 2 may be provided with a detachable top cover 21, and the vacuum pump 3 frame may be disposed above the top cover 21. The setting of the top cover can conveniently clean the photosynthetic biological reaction tube.

In order to facilitate observation of the culture solution in the photosynthetic biological reaction tube, the photosynthetic biological reaction tube 2 may be provided as a transparent glass tube or a transparent plastic tube.

In addition, in order to facilitate the transportation of the photosynthetic bioreactor, in the embodiment, a bracket 5 for erecting the liquid storage tank 1 and the photosynthetic biological reaction tube 2 may be provided, and the bottom of the bracket 5 may be provided with Universal wheel 51.

In this embodiment, in order to prevent the vacuum pump from taking too much water vapor from the photosynthetic biological reaction tube 2 when vacuuming the photosynthetic biological reaction tube 2, a steam-water separator may be disposed at a connection between the vacuum pump and the air tube. Thereby, the outflow of water vapor in the photosynthetic bioreactor 2 can be effectively prevented, and the vacuum pump can be corroded.

In this embodiment, the photosynthetic biological reaction tube may be configured as a plurality of tubes, and the outer diameter of the photosynthetic biological reaction tube is greater than or equal to 10 mm and less than or equal to 600 mm; and the height of the photosynthetic biological reaction tube is greater than or equal to 20 mm. Less than or equal to 95000mm. In the optional embodiment, the photosynthetic biological reaction tube has an outer diameter of 100 mm and a height of 200 mm.

2 is a schematic view showing the structure of another photosynthetic biological reaction tube. As shown in FIG. 2, the photosynthetic bioreactor 200 of the photosynthetic bioreactor is a coil, and the photosynthetic bioreactor has only one photosynthetic bioreactor. The photosynthetic bioreactor 2 of the photosynthetic bioreactor shown in FIG. 1 is an upright tube, and the photosynthetic bioreactor 2 can be one or more. For example, the photosynthetic bioreactor shown in FIG. 1 includes three parallels. The photosynthetic biological reaction tube 2 and the photosynthetic biological reaction tube 2 are arranged as vertical tubes.

As shown in FIG. 2, when the photosynthetic bioreactor 200 of the photosynthetic bioreactor adopts a coil, the spiral portion 201 of the photosynthetic bioreactor 200 may be caused by the photosynthetic bioreactor after long-term use due to the shape limitation of the coil. The waste residue is deposited, and the waste residue is difficult to clean due to the shape of the reaction tube 200. The deposition of the slag also affects the transparency of the reaction tube 200, so that the operator cannot visually observe the reaction state of the culture solution through the reaction tube 200. On the other hand, the upright-shaped photosynthetic bioreactor 2 shown in Fig. 1 can solve the defects of the coil-shaped reaction tube. The vertical reaction tube 2 does not have a spiral portion, and no waste residue is formed during the reaction of the culture solution, and the vertical reaction tube is easily cleaned, and the reaction tube can be kept transparent and clean even under long-term use. It is convenient for the operator to observe the reaction status of the culture solution. Moreover, the upright tube can more fully receive the illumination of the culture solution and improve the efficiency of the photosynthetic reaction.

In addition, the photosynthetic bioreactor shown in FIG. 2 includes only one coil-shaped photosynthetic bioreactor 200, and generally the length of the photosynthetic bioreactor is long, and if any part of the reaction tube 200 is seriously contaminated or damaged, Only the entire reaction tube can be replaced, resulting in high maintenance costs for the photosynthetic bioreactor. On the other hand, the length of the photosynthetic biological reaction tube 2 of the upright shape shown in Fig. 1 is shortened. When the photosynthetic bioreactor includes a plurality of upright reaction tubes 2, the plurality of reaction tubes 2 operate independently of each other without affecting each other. When one reaction tube 2 is contaminated or damaged, it is only necessary to replace the reaction tube, and the other reaction tubes can work normally. Therefore, the photosynthetic bioreactor using the vertical reaction tube is easy to maintain and the maintenance cost is low.

For example, the total length of the photosynthetic biological reaction tube of the photosynthetic bioreactor in FIG. 2 is 100 mm. When the vertical photosynthetic biological reaction tube described in FIG. 1 is used, a plurality of vertical reaction tubes may be used instead of one of the trays in FIG. Reaction tube. For example, five vertical reaction tubes are used instead of the disk-shaped reaction tubes.

In addition, the bioreactor consisting of multiple vertical tubes has a small overall volume and flexibility, and can be conveniently used in laboratories, small and medium-sized production or at home.

Industrial applicability

The present disclosure provides an optical bioreactor that enables convenient and efficient photosynthetic biological cell or tissue culture.

Claims

A photosynthetic bioreactor comprising: a liquid storage tank, a photosynthetic reaction tube disposed at a top of the liquid storage tank and disposed through the liquid storage tank, a gas pipe disposed at a top of the photosynthetic reaction tube, and the gas pipe a connected vacuum pump and a solenoid valve, a nano-aeration tube disposed in the reservoir and connected to an exhaust pipe of the vacuum pump, an exhaust fan disposed at a top of the liquid storage tank, and a cleaning air injection reservoir Air purifier for the box.
The photosynthetic bioreactor according to claim 1, further comprising a carbon dioxide supply device penetratingly connected to an exhaust pipe or a nano-aeration pipe of the vacuum pump, an in-line detecting device disposed in the liquid storage tank, and The nutrient supply of the nutrient supply is automatically added.
The photosynthetic bioreactor according to claim 2, wherein said vacuum pump, said exhaust fan, said air purifying device, said carbon dioxide supplying device, said in-line detecting device, and said automatic feeding device are both Connected to an electronic control unit that is placed outside the reservoir.
The photosynthetic bioreactor according to claim 1, wherein a top of the photosynthetic reaction tube is provided with a detachable top cover, and the vacuum pump is mounted above the top cover.
The photosynthetic bioreactor according to claim 1, wherein the photosynthetic reaction tube is provided as a transparent glass tube or a transparent plastic tube.
The photosynthetic bioreactor according to claim 1, wherein the air purifying device is provided as an air cleaner.
The photosynthetic bioreactor according to claim 1, further comprising a holder for erecting said reservoir and said photosynthetic reaction tube, the bottom of said holder being provided with a universal wheel.
The photosynthetic bioreactor according to claim 1, wherein said vacuum pump and said gas pipe The connection is provided with a steam separator.
The photosynthetic bioreactor according to claim 1, wherein the photosynthetic reaction tube is provided as a single tube or a plurality of tubes.
The photosynthetic bioreactor according to claim 1, wherein the photosynthetic reaction tube has an outer diameter of 10 mm or more and 600 mm or less; and the photosynthetic reaction tube has a height of 20 mm or more and 95000 mm or less.
The photosynthetic bioreactor according to any one of claims 1 to 10, wherein the photosynthetic reaction tube is provided as an upright tube.