CN203498392U - Lifting liquid intermittent-impregnation automatic-cyclic culture device - Google Patents

Abstract

The utility model provides a lifting liquid intermittent-impregnation automatic-cyclic culture device, and belongs to the technical field of plant tissue culture. The device comprises a main culture shelf and an auxiliary culture shelf, wherein each of the main and auxiliary culture shelves comprises two culture bottle placement frames, a culture solution storage bag hanging lifting frame, a plurality of groups of culture units, a driving system and the like; each culture unit consists of a culture bottle with an air bacterial filter, a culture solution storage bag and a connected silicon gel pipe; only the main culture shelf is provided with a motor; the auxiliary culture shelf is required to be connected with the main culture shelf to gain power; the positions of the culture solution storage bags hung on the lifting frame are changed between high positions and low positions by virtue of power of the motor controlled by a double-control circuit in a lifting way through the driving system to realize the flowing of a nutrient solution between the culture bottles and the culture solution storage bags and the automatic and intermittent circulation of sterile air exchange between the interiors and exteriors of the culture bottles, so that a culture can obtain required nutrients from the intermittently and cyclically impregnating culture solution and obtain required sterile air from the exteriors of the culture bottles. According to the device, the nutrients and the air are supplied in a coordinated way, so that the rapid and healthy growth and development of the culture is facilitated to achieve high yield and quality.

Description

Lift liquid batch submergence automated cycle culture apparatus

Technical field

The utility model provides a kind of device of plant tissue liquid culture, and a kind of lift liquid culture submergence at intermittence automated cycle culture apparatus, belongs to field of plant tissue culture technique specifically.

Technical background

Utilize the plant tissue culture technique of totipotency of plant cell, because it has, breeding is quick, production efficiency is high, can laboratory experiment condition control and the outstanding advantages such as continuous production, be rapidly developed and widespread use, as produced at seedling fast asexual propagation, Virus-free seedcane, the aspect such as secondary metabolite obtains, plant variety is cultivated, the indoor preservation of plant germplasm resource and even plant genetic engineering is all widely used.

Traditional semi-solid plant tissue culture technology, it comprises the preparation of substratum, the processes such as the inoculation of explant, shoot proliferation and the switching of taking root, hardening and transplanting, in culture medium, need to add the chemical substances such as various nutritive ingredients, especially needing to have added the agar of solidification and the sucrose etc. of carbon source is provided, is a high technology of labour intensive, cost that consumes a large amount of manpower and materials.

The later stage eighties 20th century, start mass liquid submergence tissue culture technique to study abroad, liquid culture reduces costs, is beneficial to the advantages such as scale operation because having, it is rapidly developed in the short period of time, and for solving the problems such as its Vitrification causing because of the long poor aeration of cultivation liquid soak time, deformity, in succession develop the liquid batch immersion cultural method and the multiple bio-reactor that are exclusively used in plant tissue culture.As APCS by impeller-driven intermittently complete submerged culture system, by peristaltic pump drive and timer-operated semi-automatic intermittence half submergence APCS modified version culture systems, the nutrition storage vessel being driven by air pump and two-in-one RITA intermittent immersion bioreactor, the nutrition storage vessel being driven by air pump and the culture vessel of culture vessel separate relatively independent BIT intermittent immersion bioreactor.The common feature of these systems is to utilize the pump housing to drive regularly the nutritive medium intermittent periodic pump of liquid storage system to be entered and discharge culture systems, realization is intermittently alternately immersed in nutritive medium and gas culture, even reaches and makes traditional sealing culture systems and outside air generation gaseous interchange.Applying these culture systems, to carry out intermittence submerged culture, and culture and nutritive medium and gas intermittently big area contacts, nutrient and oxygen supply fully, coordination, can obviously promote the speed of growth and the quality of culture.But in four kinds of culture systems, first two system is because pollution and space problem have limited widespread use, and latter two utilizes the air pressure of air pump to realize liquid batch submergence and the aseptic exchange of gas simultaneously, and pollution rate obviously reduces, and efficiency improves.But Tr system container volume is little, complex structure is expensive, in restriction Liao Qi developing country, applies.The 4th kind of system have concurrently liquid batch submergence and with extraneous ventilatory, and cost is relatively low, be suitable for applying in developing country, but the vent line of external connection is more more complicated, especially storing up multitube between feeding solution and culturing bottle links, and 2 sterile air filters of every group of needs, impact operation, because being used the air pressure of air pump generation and the pollution that operation does not sincerely easily cause gas leakage to bring.

In existing patent, patent of invention CN102286374A discloses a kind of plant tissue liquid automatic intermittent submerged circulative culture device, its principle adopting is similar to above-mentioned the 4th kind of group training system, but it is provided with multiple sensors electronic component and programme IC at culturing bottle inwall, increased cost, its with the design concept of the reduction cost of above-mentioned the 4th kind of culture systems to phase.And the technology providing by it is original, because not preparing air vout, in working process, perhaps can realizes indirect submergence, but be difficult to realize and outside air exchange; Patent of invention CN102618441A discloses a kind of swing type liquid batch immersion culture apparatus, and this invention adopts motor to drive swing span, and the nutrient solution that makes to be placed in swing span upper container tilts to stir the plant tissue in submergence at intermittence container.Its advantage is simple in structure, simple operation, low cost of manufacture, can realize intermittently submerged culture, weak point be also not with the function of extraneous exchange of air.

Summary of the invention

The purpose of this utility model is to provide a kind of lift liquid batch submergence automated cycle culture apparatus, solve that the performance existing in current similar technical products is good but complex structure is expensive, or can not intermittently soak and exchange ventilation and have concurrently simultaneously, maybe can reach to have concurrently intermittently to soak simultaneously and and exchange the performance of ventilation but adopt the complicated pump feed channel being connected and make operation inconvenience and easily produce to leak gas to cause the problems such as pollution.

The purpose of this utility model is achieved through the following technical solutions:

Lift liquid batch submergence automated cycle culture apparatus, comprise a main culturing rack and at least one secondary culturing rack, main, secondary culturing rack respectively comprises some groups by the culturing bottle that can place tissue culture and bottle cap band air filtration sterilization device and nutrient solution reservoir bag and is connected the cultivation unit that the two silicone tube forms, two are positioned at culturing rack both sides for the culturing bottle laying rack of fixed placement culturing bottle, one hangs up lifting support for hanging up with the nutrient solution reservoir bag of lifting nutrient solution reservoir bag between two culturing bottle laying racks, provide four parts such as transmission system of nutrient solution reservoir bag lifting hanging rack lifting motivating force to form, main culturing rack is provided with the motor of joining deceleration device, secondary culturing rack does not fill motor, it connects main culturing rack by transmission coupling spindle and obtains power, motor rotation is controlled by regularly locating dual control circuit.

The culturing bottle laying rack of major and minor culturing rack is all with eight culturing bottle laying rack montants, many culturing bottle laying rack hyphen bars and the long cube support body of culturing bottle laying rack long transverse lever through being welded into mutually, respectively there is a multi-layer rack these support body both sides, as culturing bottle laying rack, in the middle of two culturing bottle laying racks, leave a blank, for liftable nutrient solution reservoir bag lifting support is installed, it is to hang up lifting support montant by six nutrient solution reservoir bags that nutrient solution reservoir bag hangs up lifting support, many nutrient solution reservoir bags hang up lifting support hyphen bar and nutrient solution reservoir bag hangs up the long cube support body of lifting support long transverse lever through being welded into mutually, its size is advisable to be suitable for inserting leave a blank in the middle of above-mentioned culturing bottle laying rack position and liftable, on its long transverse lever, be welded with for the hook that hangs up nutrient solution reservoir bag, midpoint weldering hyphen bar and the retaining ring of top two long transverse levers draw the wireline of its lifting for firm bind, upper in the narrow end of this support body two outside, upper/lower positions is respectively installed 2 lifting support limited rollers to guarantee making this lifting process spacing, steadily with minimizing frictional force, being welded and fixed for two days of two long transverse levers of the axle two ends of these four limited rollers and culturing bottle laying rack top layer and bottom, cultivate unit and be for the device of depositing with cultured tissue culture, by being furnished with the culturing bottle of air filtration sterilization device seal bottle cap and nutrient solution reservoir bag and silicone tube, be formed by connecting.Culturing bottle is to make with the good glass of light transmission or high temperature high voltage resistant sterilizing plastics, it has larger volume, wide bottleneck is opened at top, and bottom sidewall has the nutrient solution inlet and outlet connectors with leakproof trip switch, and the supporting screw thread screwing type seal bottle cap that fills air filtration sterilization device.Nutrient solution reservoir bag is the flexible container bag of being made by the plastics film of ability autoclave sterilization, the similar drainage bag structure medically using, its base portion has osculum and is firmly connected with the sealing of silicone tube one end, and the other end of silicone tube can be socketed with the sealing of culturing bottle nutrient solution inlet and outlet connectors.The culturing bottle of cultivating unit is positioned on the interlayer of culturing bottle laying rack, and nutrient solution reservoir bag hangs up at nutrient solution reservoir bag and hangs up on the corresponding hook of lifting support, transmission system is to transmit motor motivating force to hang up lifting support and make the transmission component of its lifting to nutrient solution reservoir bag, comprise the motor of joining reduction box, motor shaft pinion, transmission shaft, be fixed on the master wheel on transmission shaft, the bent axle being connected with transmission shaft and be contained in rotating bent axle change on bent axle and with the wireline of this ring bind etc., motor is arranged on the frame top of a culturing bottle laying rack, it pushes up on the motor installation supporting plate of two long transverse lever medium positions in being welded on culturing bottle laying rack by fastened by screw, the master wheel engagement of motor shaft pinion and transmission shaft, transmission shaft is firmly connected with culturing bottle laying rack by being fixed on the bearing support of four culturing bottle laying rack long transverse lever midpoint on culturing bottle laying rack top, transmission shaft end in two outer shaft bearings is respectively welded with transmission shaft coupling mounting interface, for being installed, transmission shaft coupling provides motivating force for secondary culturing rack, wireline one end and bent axle change bind, the other end and nutrient solution reservoir bag hang up the retaining ring bind on lifting support top, for guaranteeing that bent axle rotates the steady VTOL (vertical take off and landing) of rope traction lifting support, in top position, lifting support top, two wireline position-limited wheels are installed, its wheel shaft two ends are fixed on the middle part of corresponding two culturing bottle laying rack long transverse levers, the timing location dual control circuit of controlling motor rotation is comprised of elements such as a timing change-over switch and 2 position-sensing switches and 4 each spring electromagnetism suction type limiting locks.Time switch has can a time-controlled input port and two output ports, can timing control keep input port with an output port wherein in on-state; Position-sensing switch is for pressing electricity cut-off type switch, respectively when nutrient solution storage bag hangs up lifting support and rises to predetermined extreme higher position and drop to predetermined extreme lower position, its top and bottom can be touched the position disconnecting and arrange 1, have that to press be power down function, two position-sensing switches are separately fixed at and are welded on two culturing bottle laying racks inner sides with the high-order position-sensing switch mounting plate on two montant tops of end and are welded on the low level position-sensing switch mounting plate on two montant bottoms of two culturing bottle laying racks inner sides; Spring electromagnetism suction type limiting lock has power-off and discharges the Lock function that ejects spacing locking bar; when hanging up lifting support and rise to most significant digit, nutrient solution reservoir bag arranges 4 under four montants at Si Ge angle, this bottom respectively; have while making nutrient solution reservoir bag lifting support rise to most significant digit shutdown and eject the function that lock-bit rod lock lifting support is stopped at extreme higher position, these four limiting locks are fixed on and are welded on the limiting lock mounting plate of two culturing bottle laying racks with the inner side montant base portion of end.

The utility model has carried out multinomial crucial innovation for prior art, its beneficial effect is: prior art generally adopts compressed pneumatic pump to produce the motivating force that air pressure circulates as nutrient solution, the utility model is improved to the tool kinetic energy that utilizes motor to produce machine and converts the motivating force that nutrient solution potential energy and nutrient solution self gravitation circulate as nutrient solution to, has therefore avoided in prior art, because needing with air pump to install, connecting complicated gas path pipe, existing air pressure easily to cause the problems such as risk of gas leakage generation pollution, the utility model utilize motor produce Mechanical Driven power feature innovative design the main culturing rack by a motor of a configuration can with an above secondary culturing rack linkage technique, make the utility model be more suitable for mass-producing, automatic production, the problem of " subatmospheric increase-volume power is limited, and promoting air pressure increases pipeline gas leakage risk " that has overcome prior art employing air pump and be difficult to coordinate, in the cultivation unit that prior art adopts, RITA system adopts nutrient solution storage vessel and the two-in-one cultivation unit of culture vessel, having liquid batch concurrently soaks and scavenging performance, but its complex structure is expensive, operation needs beware of fume, and BIT system is used nutrient solution storage vessel instead and culture vessel separates relatively independent " double cylinder " cultivation unit, cost reduces, but air pipe is many and connect complicated, and each bottle of cultivating unit needs with 1 air filtration sterilization device, cause operation inconvenience and have the risk that easily gas leakage is polluted, cultivation unit through the utility model innovation, retain and there is relatively independent " double cylinder " design separating, but nutritive medium storage bottle is wherein used the plastic soft bag of the similar infusion bag of medically using instead, this bag only connects a bit of silicone tube, no longer join air filtration sterilization device, whole cultivation unit structure is obviously simplified, cost reduces, use easy and simple to handle, labour intensity alleviates, efficiency improves to pollute and reduces.

Accompanying drawing explanation

Fig. 1 is the utility model example structure schematic diagram;

Fig. 2 is the timing location dual control circuit diagram of controlling motor in Fig. 1;

Fig. 3 is the left schematic side view of Fig. 1;

Fig. 4 is the schematic top plan view of Fig. 1.

In figure:

1, secondary culturing rack; 2, main culturing rack; 3, transmission shaft coupling; 4, culturing bottle laying rack; 4.1, culturing bottle laying rack montant; 4.2, culturing bottle laying rack hyphen bar; 4.3, culturing bottle laying rack long transverse lever; 5, nutrient solution reservoir bag hangs up lifting support; 5.1, nutrient solution reservoir bag hangs up lifting support montant; 5.2, nutrient solution reservoir bag hangs up lifting support hyphen bar; 5.3, nutrient solution reservoir bag hangs up lifting support long transverse lever; 6, cultivate unit; 6.1 air filtration sterilization devices; 6.2, culturing bottle; 6.3, nutrient solution; 6.4, culture; 6.5, nutrient solution inlet and outlet connectors; 6.6, nutrient solution reservoir bag; 6.7, silicone tube; 6.8, nutrient solution reservoir bag is linked with; 7, transmission system; 7.1, bent axle change; 7.2, wireline; 7.3, bent axle; 7.4, transmission shaft master wheel; 7.5, motor shaft pinion; 7.6, motor; 7.7, transmission shaft coupling mounting interface; 7.8, transmission shafts bearing; 7.9, transmission shaft; 8, wireline position-limited wheel; 9, high-order position-sensing switch; 10, high-order position-sensing switch mounting plate; 11, limiting lock mounting plate; 12, lifting support limited roller; 13, low level position-sensing switch; 14, lowest order is stopped and low level position-sensing switch mounting plate; 15, spring electromagnetism suction type limiting lock; 16, motor is installed supporting plate; 17, timing change-over switch; 18, the A position output port of timing change-over switch; 19, the B position output port of timing change-over switch.

Embodiment

Below in conjunction with accompanying drawing, embodiment of the present utility model is described in further detail.

Substratum preparation sterilizing and inoculation

Close and respectively cultivate nutrient solution inlet and outlet connectors (6.5) switch of culturing bottle base portion in unit (6) and use high temperature high voltage resistant film wrapped good, then the nutrient solution preparing (6.3) minute is installed in each culturing bottle (6.2), build bottle cap, nutrient solution reservoir bag (6.6) and the silicone tube (6.7) connecting thereof are also good by film wrapped, insert together autoclave sterilization pot and carry out sterilizing; By aseptic technique requirement, in aseptic ultra-clean operator's console, open cultivation bottle cap, Aseptic culture material (6.4) is inoculated in the culturing bottle (6.2) that nutrient solution (6.3) are housed, tighten the bottle cap of band air filtration sterilization device (6.1), get without bacterial nutrient solution reservoir bag (6.6), squeeze to discharge in bag after air that the other end cover of silicone tube it on (6.7) is received to the nutrient solution inlet and outlet connectors (6.5) of culturing bottle (6.2) is upper, and check that assurance is tightly connected.

Intermittently submergence automated cycle is cultivated

There is the unit of respectively cultivating of culture (6.4) to move and be put on the utility model device above-mentioned inoculation, it is upper that culturing bottle wherein (6.2) is placed in culturing bottle laying rack (4), and nutrient solution reservoir bag (6.6) hangs up at nutrient solution reservoir bag and hangs up on the hook that lifting support (5) is corresponding.Do not have in energising situation, nutrient solution reservoir bag lifting support (5) is parked in " extreme higher position " or " extreme lower position " only: the one, and the power-off of spring electricity magnetic absorbing-releasing limiting lock (15) discharges ejection gag lever post locking lifting support and is parked in " extreme higher position ", another is that nutrient solution bag hangs up lifting support (5) not in limited lock latched position, there is no motor-driven rising pulling force and having under self gravitation effect, under slide into that lowest order is stopped and low level position-sensing switch mounting plate (14) is upper and be stopped at this " extreme lower position ".Open the switch of the nutrient solution inlet and outlet connectors (6.5) of culturing bottle (6.2), if now nutrient solution reservoir bag hangs up lifting support (5) in extreme higher position, hang up nutrient solution reservoir bag (6.6) position on it higher than culturing bottle (6.2), nutrient solution (6.) is stayed in culturing bottle, if now nutrient solution stores and hangs up lifting support (5) in extreme lower position, the position of nutrient solution reservoir bag (6.6) is lower than culturing bottle (6.2), and nutrient solution (6.3) can flow in nutrient solution reservoir bag (6.6) from culturing bottle (6.2).

According to culture (6.4) submergence and the time span settings of submerged culture liquid not, utilize the timing function key of timing change-over switch (17) that this switch is set and hold respectively and stay corresponding time span in " A closes B and opens " position with in " A opens B and closes " position.As culture (6.4) needs the circular gap submergence of " submergence 5 minutes and not submergence 5 hours ", timing change-over switch (17) is set and in " A closes B and opens " position with in " A opens B pass " position, holds the time of staying and be respectively 5 minutes and 5 hours.Check that time set switches on power after correct, timing change-over switch (17) start by set date is introduced into " A closes B and opens " position.

If nutrient solution bag hangs up lifting support (5) and is stopped at extreme lower position while switching on power, now low level position-sensing switch (13) is pressed in de-energized by lifting support bottom, high-order position-sensing switch (9) is not pressed on position, motor (7.6) energising starts running, bent axle in lowest order (7.3) is also corresponding to be started upwards to reverse tractive and carries wireline (7.2), nutrient solution reservoir bag hangs up lifting support (5) and starts rising, low level position-sensing switch (13) is removed immediately and is pressed and forward on position to by disconnection, when bent axle (7.3) forwards extreme higher position point to, nutrient solution reservoir bag hangs up the also corresponding extreme higher position that rises to of lifting support (5), now high-order position-sensing switch (9) is hung up lifting support frame (5) top by nutrient solution reservoir bag and presses and forward open position to by connection, motor (7.6) power-off stall, nutrient solution reservoir bag hangs up lifting support (5) to be stopped rising, meanwhile, spring electromagnetism suction type limiting lock (15) power-off desorb spring ejects lock-bit bar, locking nutrient solution reservoir bag hangs up lifting support (5) locking in extreme higher position.Follow nutrient solution reservoir bag to hang up the process that lifting support (5) rises to extreme higher position, when hanging up the position of nutrient solution reservoir bag (6.6) thereon, be raised to when being placed in the position of the culturing bottle (6.2) on culturing bottle laying rack (5), in nutrient solution reservoir bag, the nutrient solution (6.3) of (6.6) starts flow to and finally all flow in culturing bottle (6.2), culture (6.4) in culturing bottle (6.2) is in nutrient solution (6.3) submergence, and the air in culturing bottle in this process (62) is discharged from outside bottle by the air filtration sterilization device (6.1) of top bottle cap.When timing change-over switch (17) is set in holding of " A close B open " position and stays the time (as 5 minutes) to arrive, its switch is transformed into " A opens B and closes " position by " A closes B and opens " position, now motor (7.6) energising starts running, bent axle in extreme higher position (7.3) also corresponding beginning torsion downwards allows wireline (7.2) decline, the release of spring electromagnetism suction type limiting lock (15) energising absorption lock-bit bar retraction, nutrient solution reservoir bag hangs up lifting support (5) and under self gravitation effect, starts decline, high-order position-sensing switch (9) is removed to press by disconnection and is changed into connection, when bent axle (7.3) forwards extreme lower position point to, nutrient solution reservoir bag hangs up the also corresponding extreme lower position of being down to of lifting support (5), now low level position-sensing switch (13) is hung up lifting support (5) low side by nutrient solution reservoir bag and presses and be transformed into open position by connection, nutrient solution reservoir bag hangs up lifting support (5) and is stopped at lowest order stop and low level position-sensing switch mounting plate (14).Follow nutrient solution reservoir bag to hang up the process that lifting support (5) drops to extreme lower position, when hanging up the position of nutrient solution reservoir bag (6.6) thereon, be raised to when being placed in the position of the culturing bottle (6.2) on culturing bottle laying rack (4), nutrient solution (6.3) in culturing bottle (6.2) starts flow to and finally all flow in nutrient solution reservoir bag (6.6), culture (6.4) in culturing bottle (6.2) does not have nutrient solution (6.3) submergence and is exposed in air, and the air in culturing bottle in this process (6.2) is sucked in bottle by the air filtration sterilization device (6.1) of top bottle cap.When timing change-over switch (17) is set in holding of " A open B close " position and stays the time (as 5 hours) to arrive, its switch is transformed into " A closes B and opens " position by " A opens B and closes " position, repeats above-mentioned lifting process.

If nutrient solution reservoir bag hangs up lifting support (5) and is stopped at extreme higher position while switching on power, now high-order position-sensing switch (9) is hung up lifting support (5) top by nutrient solution reservoir bag and presses in de-energized, low level position-sensing switch (13) is not pressed on position, motor (7.6) power-off shuts down, bent axle in extreme higher position (7.3) is also corresponding to be stopped reversing to lead carrying wireline (7.2), and nutrient solution reservoir bag hangs up lifting support (5) and continues to be stopped at extreme higher position.Now nutrient solution (6.3) continues to remain in culturing bottle (6.2), and the culture (6.4) in culturing bottle (6.2) is in nutrient solution (6.3) submergence.When timing change-over switch (17) is set in holding of " A close B open " position and stays the time (as 5 minutes) to arrive, its switch is transformed into " A opens B and closes " position by " A closes B and opens " position, now motor (7.6) energising starts running, bent axle in extreme higher position (7.3) also corresponding beginning torsion downwards allows wireline (7.2) decline, the release of spring electromagnetism suction type limiting lock (15) energising absorption lock-bit bar retraction, nutrient solution reservoir bag hangs up lifting support (5) and under self gravitation effect, starts decline, high-order position-sensing switch (9) is removed to press by disconnection and is changed into connection, when bent axle (7.3) forwards extreme lower position point to, nutrient solution reservoir bag hangs up the also corresponding extreme lower position of being down to of lifting support (5), now low level position-sensing switch (13) is hung up lifting support (5) low side by nutrient solution reservoir bag and presses and be transformed into open position by connection, nutrient solution reservoir bag hangs up lifting support (5) and is stopped at lowest order stop and low level position-sensing switch mounting plate (14).Follow nutrient solution reservoir bag to hang up the process that lifting support (5) drops to extreme lower position, when hanging up the position of nutrient solution reservoir bag (6.6) thereon, be raised to when being placed in the position of the culturing bottle (6.2) on culturing bottle laying rack (4), nutrient solution (6.3) in culturing bottle (6.2) starts flow to and finally all flow in nutrient solution reservoir bag (6.6), culture (6.4) in culturing bottle (6.2) does not have nutrient solution (6.3) submergence and is exposed in air, and the air in culturing bottle in this process (6.2) is sucked in bottle by the air filtration sterilization device (6.1) of top bottle cap.When timing change-over switch (17) is set in holding of " A open B close " position and stays the time (as 5 hours) to arrive, its switch is transformed into " A closes B and opens " position by " A opens B and closes " position, now low level position-sensing switch (13) is hung up lifting support (5) bottom by nutrient solution reservoir bag and presses in de-energized, high-order position-sensing switch (9) is not pressed on position, motor (7.6) energising starts running, bent axle in lowest order (7.3) is also corresponding to be started upwards to reverse tractive and carries wireline (7.2), nutrient solution reservoir bag hangs up lifting support (5) and starts rising, low level position-sensing switch (13) is removed immediately and is pressed and forward on position to by disconnection, when bent axle (7.3) forwards extreme higher position point to, nutrient solution reservoir bag hangs up the also corresponding extreme higher position that rises to of lifting support (5), now high-order position-sensing switch (9) is hung up lifting support (5) frame top by nutrient solution reservoir bag and presses and forward open position to by connection, motor (7.6) power-off stall, nutrient solution reservoir bag hangs up lifting support (5) to be stopped rising, meanwhile, spring electromagnetism suction type limiting lock (15) power-off desorb spring ejects lock-bit bar, locking nutrient solution reservoir bag hangs up lifting support (5) locking in extreme higher position.Follow nutrient solution reservoir bag to hang up the process that lifting support (5) rises to extreme higher position, when hanging up the position of nutrient solution reservoir bag (6.6) thereon, be raised to when being placed in the position of the culturing bottle (6.2) on culturing bottle laying rack (4), nutrient solution (6.3) in nutrient solution reservoir bag (6.6) starts flow to and finally all flow in culturing bottle (6.2), culture (6.4) in culturing bottle (6.2) is in nutrient solution (6.3) submergence, and the air in culturing bottle in this process (6.2) is discharged from outside bottle by the air filtration sterilization device (6.1) of top bottle cap.When timing change-over switch (17) is set in holding of " A close B open " position and stays the time (as 5 minutes) to arrive, its switch is transformed into " A opens B and closes " position by " A closes B and opens " position, repeats above-mentioned lifting process.

In sum, the culturing bottle (6.2) of cultivating unit (6) is fixedly placed on culturing bottle laying rack (4), nutrient solution reservoir bag (6.6) hangs up at nutrient solution reservoir bag and hangs up on lifting support (5), by hang up the automatic cycle lifting of lifting support (5) with nutrient solution reservoir bag, nutrient solution reservoir bag (6.6) is changed between a high position and low level, make to cultivate nutrient solution (6.3) in unit (6) under self gravitation effect between culturing bottle (6.2) and nutrient solution reservoir bag (6.6) automated cycle flow, simultaneously culturing bottle (6.2) inner air and outer air also can be discharged and suck by automated cycle through the air filtration sterilization device (6.1) of bottle cap is corresponding, thereby realize the automated cycle of liquid batch submergence and sterile air exchange.

The above embodiments are only that preferred implementation of the present utility model is described; the not restriction to the utility model design and scope; do not departing under the prerequisite of the utility model design concept; any technical scheme to the equal replacement of making of the present utility model and equivalent transformation formation; all should include the protection domain of the utility model requirement in; the technology contents that the utility model is claimed, is all documented in claims.

Claims (4)

1. a lift liquid batch submergence automated cycle culture apparatus, comprise a main culturing rack and at least one secondary culturing rack, main, secondary culturing rack respectively comprises some groups by the culturing bottle that can place tissue culture and bottle cap band air filtration sterilization device and nutrient solution reservoir bag and is connected the cultivation unit that the two silicone tube forms, two are positioned at culturing rack both sides for the culturing bottle laying rack of fixed placement culturing bottle, one hangs up lifting support for hanging up with the nutrient solution reservoir bag of lifting nutrient solution reservoir bag between two culturing bottle laying racks, provide nutrient solution reservoir bag to hang up the transmission system of lifting support lifting motivating force, the motor of joining deceleration device is installed on main culturing rack, and secondary culturing rack is mounted motor not, and secondary culturing rack can obtain motivating force by being connected with main culturing rack, motor is regularly being located the motivating force that under the control of dual control circuit, running produces, via drive system, make to hang up nutrient solution reservoir bag at lifting support at height, up-down deflection between lower position, it is characterized in that: by up-down mode, make nutrient solution reservoir bag at height, cyclic transformation between lower position, make to cultivate nutrient solution in unit under self gravitation effect can the nutrient solution reservoir bag of position height conversion and and the fixing culturing bottle of position height between automatic intermittent circulate transfer, the inside and outside air of culturing bottle filters and also can cyclically be sucked into and discharge by automatic intermittent through air filtration sterilization device simultaneously, realize the automated cycle that liquid batch soaks and takes a breath, culture can absorb required nutrient and from culturing bottle, obtain required sterile air from the nutrient solution of submergence at intermittence.

2. lift liquid batch submergence automated cycle culture apparatus according to claim 1, it is characterized in that: the culturing bottle top of cultivating unit is to have wide bottleneck, bottom sidewall to have the nutrient solution inlet and outlet connectors with leakproof on-off control, the vessel with larger volume made from the good glass of light transmission or high temperature high voltage resistant sterilizing plastics, and joins the screw thread screwing type seal bottle cap that can fill air filtration sterilization device interface; Nutrient solution reservoir bag is the flexible container bag of being made by the plastics film of ability autoclave sterilization, and its base portion has osculum and is firmly connected with the sealing of silicone tube one end, and top has can be for being linked with of hanging up.

3. lift liquid batch submergence automated cycle culture apparatus according to claim 1; it is characterized in that: on main culturing rack, secondary culturing rack, be provided with transmission shaft coupling mounting interface, by this interface, transmission shaft coupling be installed and carried out connecting between culturing rack realizing and make secondary culturing rack obtain motivating force and be installed in addition with secondary culturing rack dilatation from main culturing rack to carry out large-scale production.

4. lift liquid batch submergence automated cycle culture apparatus according to claim 1, is characterized in that: the timing location dual control circuit of regulation and control motor is comprised of timing change-over switch and position-sensing switch and spring electromagnetism suction type limiting lock; Timing change-over switch is subject to a time-controlled input port and two output ports, can control conversion or keep input port and the time of an output port in on-state wherein; Position-sensing switch has the performance of the power-off of pressing and decompress(ion) energising, on nutrient solution storage bag lifting support top, rise to respectively the position that can touch disconnected Ji when predetermined extreme higher position and low side drop to predetermined extreme lower position and respectively install 1, having that lifting support presses is the function that i.e. energising start was shut down and left in power-off; Spring electromagnetism suction type limiting lock has power-off and discharges ejection locking bar lock-bit and switch on to adsorbing and regain lock-bit bar solution lock-bit performance; under the montant at four angles that respectively mutually should frame bottom when nutrient solution reservoir bag lifting support rises to most significant digit, respectively install 1, there is nutrient solution reservoir bag lifting support and rise to most significant digit and have a power failure and while shutting down, can eject locking lifting support position and avoid this downslide and the release of energising absorption withdrawal lock-bit bar allow the function of lifting support downslide.

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