CN201420083Y - A culture reactor for submerging plant tissues and organs intermittently - Google Patents

Abstract

The utility model discloses a cultivation reaction vessel for intermittent plant tissue and organ immersion. The cultivation reaction vessel comprises an inflator pump (2) connected with a time controller (1), and further comprises a liquid storage tank (5) and a main reaction tank (7); the inflator pump (2) is in sealing connection with an inlet of the liquid storage tank (5) through an air inletpipe (3); an air degerming device I (4) is arranged on the air inlet pipe (3); an outlet of the liquid storage tank (5) is in sealed connection with an inlet of the main reaction tank (7) through a connecting pipe (6); a gas outlet is formed on the main reaction tank (7); and the gas outlet is connected with an air degerming device II (9). The cultivation reaction vessel has the characteristics of simple structure, low manufacturing cost, and good practical use effect.

Description

A culture reactor for submerging plant tissues and organs intermittently

technical field

The utility model relates to a culture reactor, in particular to a culture reactor for intermittently submerging plant tissues and organs (such as callus, hairy root, adventitious bud, etc.).

Background technique

At present, in the field of plant tissue culture, the culture method mainly refers to solid or semi-solid culture in which agar is used as a support in a culture bottle. This culture method needs to consume a large amount of agar, and a large number of containers need to be handled when filling and cleaning, so it consumes a lot of labor, resulting in high production costs. Although the liquid culture method has the advantages of easy renewal, no need to change containers, convenient filter sterilization, convenient cleaning, convenient application of large containers, and reduced time for seedling transfer, etc.; however, the disadvantages of liquid culture are also relatively concentrated, such as easy hypoxia, long-term immersion in Plant organs in liquid are easy to vitrify, plant organs are sensitive to stirring force, and require complex equipment.

So far, there is no simple, practical, and industrialized culture reactor and corresponding automation device. The research on plant tissue culture reactors mainly includes the following aspects: 1. Improvements to traditional stirred tanks and airlift tanks; however, the phenomenon of vitrification cannot be solved because submerged culture is still used. 2. Laminar flow reactors, such as shallow layer and drum culture reactors; although vitrification is reduced, the culture effect is not ideal. 3. Spraying and atomization reactors, the cultivating effect of such reactors is relatively ideal, and there are related patent applications, such as the "Plant Organ Biological Cultivation Reactor" with the patent No. Rotating sieve plate mist flow plant tissue culture reactor”, and the patent No. 96207831.X “elastic valve sieve plate plant tissue culture reactor”. However, with the growth of the plants in the later stage of the cultivation, the misty culture solution is difficult to distribute evenly, which seriously affects the growth of the plants. 4. Intermittent immersion reactor, this type of reactor mainly uses culture medium to cultivate plant tissue by intermittent immersion, supplies nutrients to plants during immersion, and provides enough oxygen to plants during intermittent periods. Because this culture method can better solve the problem of vitrification, and the nutrients can be well transferred along with the immersion of plant tissue; therefore, this culture method can well solve various problems in plant tissue culture . There are many studies on this kind of culture method abroad, and there are related patent applications for this kind of reactor in China, such as the "Airlift Periodic Immersion Illumination Plant Cell Tissue and Organ Culture Reactor" with the patent number 98205797. "Air-lift periodic immersion light plant cell tissue organ culture method and culture reactor". However, the structure of the above-mentioned equipment is relatively complicated, and the manufacturing cost is high, which makes it difficult to popularize.

Utility model content

The technical problem to be solved by the utility model is to provide a culture reactor for intermittently submerging plant tissues and organs with simple structure, low manufacturing cost and good practical effect.

In order to solve the above-mentioned technical problems, the utility model provides a culture reactor for submerging plant tissues and organs intermittently, which includes an air pump connected with a time controller, and also includes a liquid storage tank and a main reaction tank; The inlet of the tank is sealed and connected, and an air sterilizer 1 is arranged on the air inlet pipe; the outlet of the liquid storage tank is connected with the inlet of the main reaction tank (7) by a connecting pipe, and the main reaction tank is provided with a gas outlet, and the gas outlet is connected to the air outlet. Sterilizer II is connected.

As an improvement of the cultivation reactor for intermittently submerging plant tissues and organs of the present invention: the main reaction tank is arranged on a height-adjustable support frame.

As a further improvement of the cultivation reactor for intermittently submerging plant tissues and organs of the present invention: the liquid storage tank is provided with a liquid inlet, and a sealing plug I is arranged at the liquid inlet.

As a further improvement of the cultivation reactor for intermittently submerging plant tissues and organs of the present invention: the main reaction tank is provided with a feeding port, and a sealing plug II is arranged at the feeding port.

As a further improvement of the cultivation reactor for intermittently submerging plant tissues and organs of the present invention: a filter screen is arranged in the inlet of the reaction tank.

As a further improvement of the cultivation reactor for intermittently submerging plant tissues and organs of the present invention: the inlet of the main reaction tank is located at the bottom of the main reaction tank.

As a further improvement of the cultivation reactor for intermittently submerging plant tissues and organs of the present invention: the outlet of the liquid storage tank is located at the bottom of the liquid storage tank.

The cultivation reactor for intermittently immersing plant tissues and organs of the utility model uses a time controller to control the on-off of the current, so as to realize whether the air pump is working or not. When the inflatable pump works, under the action of air pressure, the culture solution in the liquid storage tank enters the main reaction tank, thereby infiltrating the plant tissue in the main reaction tank, and the gas in the main reaction tank can be discharged from the gas outlet. When the air pump stops working, the culture solution in the main reaction tank returns to the liquid storage tank under the action of gravity. The height difference between the main reaction tank and the liquid storage tank can be adjusted by adjusting the height of the support frame, so as to ensure that the height of the inlet of the main reaction tank is always higher than the liquid level in the liquid storage tank. In this way, the plant tissue in the main reaction tank can be submerged intermittently by the culture solution. The cultivation reactor of the utility model has simple structure, low manufacturing cost, and is convenient for large-scale production and popularization and use.

Description of drawings

Below in conjunction with accompanying drawing, the specific embodiment of the present utility model is described in further detail.

Fig. 1 is a structural schematic diagram of a culture reactor for intermittently submerging plant tissues and organs of the present invention.

Detailed ways

Figure 1 shows a culture reactor for intermittently submerging plant tissues and organs, which mainly includes a time controller 1, an air pump 2, a liquid storage tank 5 and a main reaction tank 7, etc. The time controller 1 is connected to the air pump 2 through a wire , The work of the air pump 2 is controlled by the time controller 1.

The liquid storage tank 5 is a conical bottle with a volume of 1L, and the top of the liquid storage tank 5 is provided with a liquid inlet, and a sealing plug 151 is set at this liquid inlet; The inner cavity of liquid tank 5. An inlet pipe 52 is provided on the side wall of the liquid storage tank 5 (near the top of the liquid storage tank 5 ), and the inlet pipe 52 communicates with the inner chamber of the liquid storage tank 5 . One end of the air intake pipe 3 is sealed and connected with the air pump 2, and the other end is sealed and connected with the inlet pipe 52; the air intake pipe 3 is provided with an air sterilizer 14. An outlet pipe 53 is provided at the junction of the side wall of the liquid storage tank 5 and the bottom of the liquid storage tank 5 . The liquid storage tank 5 includes an inlet pipe 52 and an outlet pipe 53 all made of transparent glass with good light transmission, the inlet pipe 3 is made of a silicone tube, and the air sterilizer 14 can be an air sterilizer with a filter screen aperture of 0.22um , Sealing plug I 51 selects silica gel plug for use.

The main reaction tank 7 is a spherical bottle with a volume of 1L, and the top of the main reaction tank 7 is provided with a feed port, and a sealing plug II 71 is set at this feed port; the plant tissue enters the main reaction through the feed port. The inner cavity of the tank 7. The sidewall (near the top of main reaction tank 7) is provided with gas outlet pipe 72 at the sidewall of main reaction tank 7, and one end of connecting pipe 8 is connected with gas outlet pipe 72 sealing, and the other end is connected with air sterilizer II 9 sealingly. The bottom of main reaction tank 7 is provided with reaction tank inlet pipe 73, and reaction tank inlet pipe 73 communicates with the inner chamber of main reaction tank 7; Sealed connected. Filter screen 74 is set in reaction tank inlet pipe 73, and this filter screen 74 is the nylon mesh of ball shape, and the effect of filter screen 74 is to stop the plant tissue in the main reaction tank 7 from falling in the connecting pipe 6, promptly stops plant tissue from passing through The connecting pipe 6 enters the liquid storage tank 5 . The main reaction tank 7 includes the gas outlet pipe 72 and the reaction tank inlet pipe 73, both of which are made of transparent glass with good light transmission, the connecting pipe 6 and the connecting pipe 8 are all made of silicone tubes, and the air sterilizer II 9 can be selected with a filter mesh aperture For a 0.22um air sterilizer, the sealing plug II 71 uses a silicone plug.

The whole main reaction tank 7 is arranged on the height-adjustable support frame 10, and the height of the main reaction tank 7 is adjusted by adjusting the height of the support frame 10, thereby ensuring that the bottom of the main reaction tank 7 is always higher than the cultivation in the liquid storage tank 5. liquid level height.

The actual working process of the cultivation reactor for intermittently submerging plant tissues and organs of the present utility model is as follows:

1. Open the sealing plug I 51, put an appropriate amount of culture solution into the liquid storage tank 5, and immediately plug the sealing plug I 51. Then liquid storage tank 5 (comprising sealing plug I 51, inlet pipe 52 and outlet pipe 53) together with connecting pipe 6 and main reaction tank 7 (comprising sealing plug II 71, gas outlet pipe 72, reaction tank inlet pipe 73 and filter screen 74) Carry out moist heat sterilization at 121°C for 25 minutes.

2. Under sterile conditions, open the sealing plug II 71, put the plant tissues and organs to be cultivated into the main reaction tank 7, and immediately plug the sealing plug II 71.

3. Adjust the height of the support frame 10 so that the bottom of the main reaction tank 7 is higher than the liquid level of the culture solution in the liquid storage tank 5 .

4. The air pump 2 works intermittently under the control of the time controller 1.

When the air pump 2 was in working order, the gas entered the intake pipe 3 and was sterilized by the air sterilizer 14, and then entered the inner cavity of the liquid storage tank 5 through the inlet pipe 52; Through the outlet pipe 53, the connecting pipe 6, and the reaction tank inlet pipe 73, it enters the main reaction tank 7 successively, so that the plant tissues and organs in the main reaction tank 7 are immersed in the culture solution. The gas in the main reaction tank 7 is discharged through the gas outlet pipe 72, the connecting pipe 8 and the air sterilizer II 9 successively.

When the air pump 2 stopped working, the culture solution in the main reaction tank 7 was returned to the liquid storage tank 5 through the reaction tank inlet pipe 73, the connecting pipe 6 and the outlet pipe 53 successively under the action of its own gravity; A filter screen 74 is arranged in the pipe 73, so the plant tissues and organs in the main reaction tank 7 will not flow back into the liquid storage tank 5 along with the culture solution. At this moment, negative pressure is formed in the main reaction tank 7, and the gas of the outside enters in the main reaction tank 7 along the connecting pipe 8 and the gas outlet pipe 72 after being sterilized by the air sterilizer II 9.

Like this, the plant tissues and organs in the main reaction tank 7 have just completed a cycle that is submerged intermittently. The time of the above-mentioned cycle can be adjusted by adjusting the time controller 1.

Finally, it should also be noted that what is listed above is only a specific embodiment of the present invention. Apparently, the utility model is not limited to the above embodiments, and many variations are possible. All deformations that a person skilled in the art can derive or associate directly from the content disclosed in the utility model shall be considered as the protection scope of the utility model.

Claims (7)

1, a kind of cultivation reactor of submergence at intermittence plant tissue organ is characterized in that: comprise the aerator pump (2) that links to each other with time controller (1), also comprise container for storing liquid (5) and main treatment tank (7); Aerator pump (2) links to each other with the inlet seal of container for storing liquid (5) by inlet pipe (3), and air sterilization device I (4) is set on inlet pipe (3); The outlet of container for storing liquid (5) links to each other with the inlet seal of main treatment tank (7) by pipe connecting (6), and main treatment tank (7) is provided with pneumatic outlet, and described pneumatic outlet links to each other with air sterilization device II (9).

2, the cultivation reactor of a kind of submergence at intermittence plant tissue organ according to claim 1, it is characterized in that: main treatment tank (7) is arranged on the height-adjustable bracing frame (10).

3, the cultivation reactor of a kind of submergence at intermittence plant tissue organ according to claim 1 and 2, it is characterized in that: container for storing liquid (5) is provided with fluid inlet, and the fluid inlet place is provided with sealing plug I (51).

4, the cultivation reactor of a kind of submergence at intermittence plant tissue organ according to claim 3, it is characterized in that: main treatment tank (7) is provided with opening for feed, and the opening for feed place is provided with sealing plug II (71).

5, the cultivation reactor of a kind of submergence at intermittence plant tissue organ according to claim 4 is characterized in that: filtering net (74) is set in the import of retort (7).

6, the cultivation reactor of a kind of submergence at intermittence plant tissue organ according to claim 5 is characterized in that: the import of main treatment tank (7) is positioned at the bottom of main treatment tank (7).

7, the cultivation reactor of a kind of submergence at intermittence plant tissue organ according to claim 6 is characterized in that: the outlet of container for storing liquid (5) is positioned at the bottom of container for storing liquid (5).

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