CN201420083Y - Cultivation reaction vessel for intermittent plant tissue and organ immersion - Google Patents
Cultivation reaction vessel for intermittent plant tissue and organ immersion Download PDFInfo
- Publication number
- CN201420083Y CN201420083Y CN200920116327XU CN200920116327U CN201420083Y CN 201420083 Y CN201420083 Y CN 201420083Y CN 200920116327X U CN200920116327X U CN 200920116327XU CN 200920116327 U CN200920116327 U CN 200920116327U CN 201420083 Y CN201420083 Y CN 201420083Y
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- plant tissue
- treatment tank
- main treatment
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- submergence
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- 210000000056 organ Anatomy 0.000 title claims abstract description 32
- 238000007654 immersion Methods 0.000 title abstract description 5
- 239000007788 liquid Substances 0.000 claims abstract description 42
- 238000007789 sealing Methods 0.000 claims abstract description 21
- 230000001954 sterilising effect Effects 0.000 claims description 15
- 238000004659 sterilization and disinfection Methods 0.000 claims description 15
- 238000005276 aerator Methods 0.000 claims description 14
- 238000001914 filtration Methods 0.000 claims description 8
- 239000012530 fluid Substances 0.000 claims description 7
- 230000008676 import Effects 0.000 claims description 5
- 230000000694 effects Effects 0.000 abstract description 9
- 238000004519 manufacturing process Methods 0.000 abstract description 5
- 235000015097 nutrients Nutrition 0.000 description 13
- 239000007789 gas Substances 0.000 description 11
- 238000000034 method Methods 0.000 description 8
- 230000006872 improvement Effects 0.000 description 7
- 238000004161 plant tissue culture Methods 0.000 description 5
- 230000001105 regulatory effect Effects 0.000 description 3
- 229920001817 Agar Polymers 0.000 description 2
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 2
- 239000008272 agar Substances 0.000 description 2
- 230000005540 biological transmission Effects 0.000 description 2
- 125000004122 cyclic group Chemical group 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 239000011521 glass Substances 0.000 description 2
- 238000009630 liquid culture Methods 0.000 description 2
- 229920001296 polysiloxane Polymers 0.000 description 2
- 239000000741 silica gel Substances 0.000 description 2
- 229910002027 silica gel Inorganic materials 0.000 description 2
- 239000007921 spray Substances 0.000 description 2
- 238000004017 vitrification Methods 0.000 description 2
- 206010020649 Hyperkeratosis Diseases 0.000 description 1
- 239000004677 Nylon Substances 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 238000009924 canning Methods 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 210000003746 feather Anatomy 0.000 description 1
- 230000002070 germicidal effect Effects 0.000 description 1
- 230000005484 gravity Effects 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 238000011031 large-scale manufacturing process Methods 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- 229920001778 nylon Polymers 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 230000008635 plant growth Effects 0.000 description 1
- 230000001863 plant nutrition Effects 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
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Abstract
The utility model discloses a cultivation reaction vessel for intermittent plant tissue and organ immersion. The cultivation reaction vessel comprises an inflator pump (2) connected with a time controller (1), and further comprises a liquid storage tank (5) and a main reaction tank (7); the inflator pump (2) is in sealing connection with an inlet of the liquid storage tank (5) through an air inletpipe (3); an air degerming device I (4) is arranged on the air inlet pipe (3); an outlet of the liquid storage tank (5) is in sealed connection with an inlet of the main reaction tank (7) through a connecting pipe (6); a gas outlet is formed on the main reaction tank (7); and the gas outlet is connected with an air degerming device II (9). The cultivation reaction vessel has the characteristics of simple structure, low manufacturing cost, and good practical use effect.
Description
Technical field
The utility model relates to a kind of cultivation reactor, particularly a kind of intermittently cultivation reactor of submergence plant tissue organ (for example callus, hairly root, indefinite bud etc.) that is used for.
Background technology
In the plant tissue culture field, training method mainly is meant in culturing bottle solid or the semisolid cultivation of agar as upholder at present.This training method need expend a large amount of agar, and need handle a large amount of containers when can and cleaning, therefore expends a large amount of labor forces, thereby causes production cost high.Though the liquid culture mode have upgrade easily, need not to change container, make things convenient for filtration sterilization, clean convenient, be convenient to tun and use, reduce advantages such as changeing the seedling time; But shortcoming is also more concentrated when liquid culture, for example easily anoxic, for a long time be immersed in the easy vitrifying of plant organ in the liquid, plant organ is to the whipping force sensitivity and need complex apparatus etc.
So far still there are not simple, practical, easy industrialized cultivation reactor and corresponding automated installation.Research for the plant tissue culture reactor mainly contains following aspect: 1, to the improvement of conventional stirred-tank, gas lift jar; But owing to still adopt immersion to cultivate, so vitrification phenomenon can't solve.2, course flow reactor is cultivated reactor as shallow-layer formula and rotary drum; Though reduced vitrification phenomenon, but culture effect is undesirable.3, spray, atomizing type reactor, this type of reactor culture effect is comparatively desirable, relevant patent application is also arranged now, as the patent No. is that 97220500.4 " plant organ biological culture reactor " and the patent No. are 96204509.8 " the board-like spray flow plant tissue culture of multilayer trundle screen reactor ", and the patent No. is 96207831.X " a feather valve sieve plate plant tissue culture reactor ".But this kind training method is in the growth of late stage of culture along with plant, and vaporific nutrient solution is difficult to be evenly distributed, and has had a strong impact on the growth of plant.4, submergence at intermittence reactor, this type of reactor mainly is to utilize nutrient solution that the submergence at intermittence of plant tissue is cultivated, and supplies with plant nutrition during submergence, provides plant enough oxygen again in the time of intermittently.Because this training method preferably resolves vitrified problem, and nutritive substance is along with the submergence to plant tissue also can well be transmitted; Therefore this training method can well solve the various difficult problems in the plant tissue culture.This type of training method foreign study is more, and this kind reactor also has relevant patent application at home, as the patent No. is 98205797.0 " Airlift cyclic immersion lighting plant cell tissue organ cultivation reactor ", and application number is 98102396.7 " Airlift cyclic immersion lighting plant cell tissue organ cultural method and cultivate reactor ".But aforesaid device structure is comparatively complicated, and the manufacturing cost height causes being difficult for popularizing.
The utility model content
The technical problems to be solved in the utility model provides a kind of cultivation reactor of simple in structure, cheap for manufacturing cost, submergence at intermittence plant tissue organ that practical effect is good.
In order to solve the problems of the technologies described above, the utility model provides a kind of cultivation reactor of submergence at intermittence plant tissue organ, comprises the aerator pump that links to each other with time controller, also comprises container for storing liquid and main treatment tank; Aerator pump links to each other with the inlet seal of container for storing liquid by inlet pipe, and air sterilization device I is set on inlet pipe; The outlet of container for storing liquid links to each other by the inlet seal of pipe connecting with main treatment tank (7), and main treatment tank is provided with pneumatic outlet, and pneumatic outlet links to each other with air sterilization device II.
Improvement as the cultivation reactor of submergence at intermittence plant tissue organ of the present utility model: main treatment tank is arranged on the height-adjustable bracing frame.
Further improvement as the cultivation reactor of submergence at intermittence plant tissue organ of the present utility model: container for storing liquid is provided with fluid inlet, and the fluid inlet place is provided with sealing plug I.
Further improvement as the cultivation reactor of submergence at intermittence plant tissue organ of the present utility model: main treatment tank is provided with opening for feed, and the opening for feed place is provided with sealing plug II.
Further improvement as the cultivation reactor of submergence at intermittence plant tissue organ of the present utility model: in the import of retort, filtering net is set.
Further improvement as the cultivation reactor of submergence at intermittence plant tissue organ of the present utility model: the import of main treatment tank is positioned at the bottom of main treatment tank.
Further improvement as the cultivation reactor of submergence at intermittence plant tissue organ of the present utility model: the outlet of container for storing liquid is positioned at the bottom of container for storing liquid.
The cultivation reactor of submergence at intermittence plant tissue organ of the present utility model utilizes time controller to come the break-make of control current, thereby the work that realizes the control aerator pump whether.When aerator pump was worked, under the effect of air pressure, the nutrient solution in the container for storing liquid entered in the main treatment tank, thereby soaked into the plant tissue in the main treatment tank, and the gas in the main treatment tank can be discharged from from pneumatic outlet.When aerator pump quit work, the nutrient solution in the main treatment tank was back to container for storing liquid under the effect of gravity.Can regulate difference of altitude between main treatment tank and the container for storing liquid by the height of regulating bracing frame, thereby the height that guarantees the main treatment tank import is higher than the liquid level in the container for storing liquid all the time.Like this, just can realize that the interior plant tissue of main treatment tank is by intermittently submergence of nutrient solution.Cultivation reactor of the present utility model, simple in structure, cost of manufacture is cheap, be convenient to large-scale production and promote the use of.
Description of drawings
Below in conjunction with accompanying drawing embodiment of the present utility model is described in further detail.
Fig. 1 is the structural representation of the cultivation reactor of submergence at intermittence plant tissue organ of the present utility model.
Embodiment
Fig. 1 has provided a kind of cultivation reactor of submergence at intermittence plant tissue organ, mainly comprises time controller 1, aerator pump 2, container for storing liquid 5 and main treatment tank 7 etc., and time controller 1 links to each other with aerator pump 2 by lead, and whether the work of aerator pump 2 is controlled by time controller 1.
Container for storing liquid 5 is that a volume is the conical bottle of 1L, and the top of container for storing liquid 5 is provided with fluid inlet, at this fluid inlet place sealing plug I 51 is set; Nutrient solution fluid inlet thus is added into the inner chamber of container for storing liquid 5.Sidewall (near the top of container for storing liquid 5) at container for storing liquid 5 is provided with inlet pipe 52, and this inlet pipe 52 communicates with the inner chamber of container for storing liquid 5.One end of inlet pipe 3 links to each other with aerator pump 2 sealings, and the other end links to each other with inlet pipe 52 sealings; On inlet pipe 3, be provided with air sterilization device I 4.Intersection in container for storing liquid 5 sidewalls and container for storing liquid 5 bottoms is provided with outlet pipe 53.Container for storing liquid 5 comprises that inlet pipe 52 and outlet pipe 53 make by the good transparent glass of light transmission, and inlet pipe 3 is selected silicone tube for use, and air sterilization device I 4 can select for use opening of sieve directly to be the air sterilization device of 0.22um, and sealing plug I 51 selects silica gel plug for use.
Whole main treatment tank 7 is arranged on the height-adjustable bracing frame 10, adjusts the height of main treatment tank 7 by the height of regulating bracing frame 10, thereby guarantees that the bottom of main treatment tank 7 is higher than the liquid level of the nutrient solution in the container for storing liquid 5 all the time.
The cultivation reactor of submergence at intermittence plant tissue organ of the present utility model, practical work process is as follows:
1, open sealing plug I 51, an amount of nutrient solution is put into container for storing liquid 5 after, filled in sealing plug I 51 at once.Then container for storing liquid 5 (comprising sealing plug I 51, inlet pipe 52 and outlet pipe 53) is carried out (comprising sealing plug II 71, gas outlet tube 72, retort inlet pipe 73 and filtering net 74) moist heat sterilization of 25min under 121 ℃ together with pipe connecting 6 and main treatment tank 7.
2, under aseptic condition, open sealing plug II 71, plant tissue organ to be cultivated is put into main treatment tank 7 after, filled in sealing plug II 71 at once.
3, adjust the height of bracing frame 10, make the bottom of main treatment tank 7 be higher than the liquid level of the nutrient solution in the container for storing liquid 5.
4, aerator pump 2 carries out discontinuous operation under the control of time controller 1.
When aerator pump 2 was in running order, gas entered inlet pipe 3 and carries out degerming by air sterilization device I 4 and handle, and enters container for storing liquid 5 inner chambers by inlet pipe 52 then; Nutrient solution in the container for storing liquid 5 enters in the main treatment tank 7 by outlet pipe 53, pipe connecting 6, retort inlet pipe 73 under the effect of air pressure successively, and the plant tissue organ in the main treatment tank 7 is immersed in the nutrient solution.Gas in the main treatment tank 7 then is discharged from by gas outlet tube 72, connecting leg 8 and air sterilization device II 9 successively.
When aerator pump 2 quit work, the nutrient solution in the main treatment tank 7 was back in the container for storing liquid 5 by retort inlet pipe 73, pipe connecting 6, outlet pipe 53 under the effect of self gravitation successively; Owing to be provided with filtering net 74 in the retort inlet pipe 73, so the plant tissue organ in the main treatment tank 7 can be along with nutrient solution is back in the container for storing liquid 5.This moment, the main treatment tank 7 interior negative pressure that form entered in the main treatment tank 7 along connecting leg 8, gas outlet tube 72 after the germicidal treatment of extraneous gas through air sterilization device II 9 successively.
Like this, the plant tissue organ in the main treatment tank 7 has just been finished one by intermittently buried circulation.Can regulate the above-mentioned round-robin time by regulating time controller 1.
At last, it is also to be noted that what more than enumerate only is a specific embodiment of the present utility model.Obviously, the utility model is not limited to above embodiment, and many distortion can also be arranged.All distortion that those of ordinary skill in the art can directly derive or associate from the disclosed content of the utility model all should be thought protection domain of the present utility model.
Claims (7)
1, a kind of cultivation reactor of submergence at intermittence plant tissue organ is characterized in that: comprise the aerator pump (2) that links to each other with time controller (1), also comprise container for storing liquid (5) and main treatment tank (7); Aerator pump (2) links to each other with the inlet seal of container for storing liquid (5) by inlet pipe (3), and air sterilization device I (4) is set on inlet pipe (3); The outlet of container for storing liquid (5) links to each other with the inlet seal of main treatment tank (7) by pipe connecting (6), and main treatment tank (7) is provided with pneumatic outlet, and described pneumatic outlet links to each other with air sterilization device II (9).
2, the cultivation reactor of a kind of submergence at intermittence plant tissue organ according to claim 1, it is characterized in that: main treatment tank (7) is arranged on the height-adjustable bracing frame (10).
3, the cultivation reactor of a kind of submergence at intermittence plant tissue organ according to claim 1 and 2, it is characterized in that: container for storing liquid (5) is provided with fluid inlet, and the fluid inlet place is provided with sealing plug I (51).
4, the cultivation reactor of a kind of submergence at intermittence plant tissue organ according to claim 3, it is characterized in that: main treatment tank (7) is provided with opening for feed, and the opening for feed place is provided with sealing plug II (71).
5, the cultivation reactor of a kind of submergence at intermittence plant tissue organ according to claim 4 is characterized in that: filtering net (74) is set in the import of retort (7).
6, the cultivation reactor of a kind of submergence at intermittence plant tissue organ according to claim 5 is characterized in that: the import of main treatment tank (7) is positioned at the bottom of main treatment tank (7).
7, the cultivation reactor of a kind of submergence at intermittence plant tissue organ according to claim 6 is characterized in that: the outlet of container for storing liquid (5) is positioned at the bottom of container for storing liquid (5).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200920116327XU CN201420083Y (en) | 2009-03-24 | 2009-03-24 | Cultivation reaction vessel for intermittent plant tissue and organ immersion |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200920116327XU CN201420083Y (en) | 2009-03-24 | 2009-03-24 | Cultivation reaction vessel for intermittent plant tissue and organ immersion |
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| CN201420083Y true CN201420083Y (en) | 2010-03-10 |
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| CN200920116327XU Expired - Fee Related CN201420083Y (en) | 2009-03-24 | 2009-03-24 | Cultivation reaction vessel for intermittent plant tissue and organ immersion |
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102150624A (en) * | 2011-04-29 | 2011-08-17 | 南京工业大学 | Tissue culture and rapid propagation method of pinellia genus plant |
| CN103120124A (en) * | 2013-02-04 | 2013-05-29 | 南京工业大学 | Method and device for continuously obtaining plant secondary metabolites through tissue culture |
| CN103120125A (en) * | 2013-02-04 | 2013-05-29 | 南京工业大学 | Method and device for high-throughput screening of salt-tolerant plants |
| CN103875531A (en) * | 2014-03-21 | 2014-06-25 | 北京农学院 | Cyclic immersion type bioreactor device for strawberries and use method of device |
| CN103999777A (en) * | 2013-02-04 | 2014-08-27 | 南京工业大学大丰海洋产业研究院 | Method for continuously obtaining pinellia ternata seedling secondary metabolites through tissue culture |
| CN104770304A (en) * | 2015-04-30 | 2015-07-15 | 南京博方生物科技有限公司 | Plant bio-reactor and using method thereof |
| CN105104191A (en) * | 2015-08-11 | 2015-12-02 | 湖南科技学院 | Tissue culture reactor |
| CN105191798A (en) * | 2015-09-29 | 2015-12-30 | 中国科学院植物研究所 | Temporary-immersion lily scale rapid-propagation method |
| CN105210866A (en) * | 2015-09-29 | 2016-01-06 | 中国科学院植物研究所 | A kind of intermittent immersed orchid protocorms propagation quick-breeding method |
| CN105340733A (en) * | 2015-09-29 | 2016-02-24 | 中国科学院植物研究所 | Intermittent immersion type gesneriaceae leave adventitious buds induction method |
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2009
- 2009-03-24 CN CN200920116327XU patent/CN201420083Y/en not_active Expired - Fee Related
Cited By (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102150624B (en) * | 2011-04-29 | 2013-05-15 | 南京工业大学 | Tissue culture and rapid propagation method of pinellia genus plant |
| CN102150624A (en) * | 2011-04-29 | 2011-08-17 | 南京工业大学 | Tissue culture and rapid propagation method of pinellia genus plant |
| CN103120124A (en) * | 2013-02-04 | 2013-05-29 | 南京工业大学 | Method and device for continuously obtaining plant secondary metabolites through tissue culture |
| CN103120125A (en) * | 2013-02-04 | 2013-05-29 | 南京工业大学 | Method and device for high-throughput screening of salt-tolerant plants |
| CN103999777A (en) * | 2013-02-04 | 2014-08-27 | 南京工业大学大丰海洋产业研究院 | Method for continuously obtaining pinellia ternata seedling secondary metabolites through tissue culture |
| CN103999777B (en) * | 2013-02-04 | 2017-03-08 | 南京工业大学大丰海洋产业研究院 | The method that Rhizoma Pinelliae seedling secondary metabolite is persistently obtained by tissue culture |
| CN103875531B (en) * | 2014-03-21 | 2017-01-18 | 北京农学院 | Cyclic immersion type bioreactor device for strawberries and use method of device |
| CN103875531A (en) * | 2014-03-21 | 2014-06-25 | 北京农学院 | Cyclic immersion type bioreactor device for strawberries and use method of device |
| CN104770304A (en) * | 2015-04-30 | 2015-07-15 | 南京博方生物科技有限公司 | Plant bio-reactor and using method thereof |
| CN105104191A (en) * | 2015-08-11 | 2015-12-02 | 湖南科技学院 | Tissue culture reactor |
| CN105104191B (en) * | 2015-08-11 | 2017-07-14 | 湖南科技学院 | A kind of tissue cultures reactor |
| CN105210866A (en) * | 2015-09-29 | 2016-01-06 | 中国科学院植物研究所 | A kind of intermittent immersed orchid protocorms propagation quick-breeding method |
| CN105340733A (en) * | 2015-09-29 | 2016-02-24 | 中国科学院植物研究所 | Intermittent immersion type gesneriaceae leave adventitious buds induction method |
| CN105191798A (en) * | 2015-09-29 | 2015-12-30 | 中国科学院植物研究所 | Temporary-immersion lily scale rapid-propagation method |
| CN105191798B (en) * | 2015-09-29 | 2017-09-22 | 中国科学院植物研究所 | A kind of intermittent immersed lily bud scale propagation quick-breeding method |
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| C14 | Grant of patent or utility model | ||
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Granted publication date: 20100310 Termination date: 20110324 |