CN201809355U - Intermittent submerged open-close plant bioreactor - Google Patents

Abstract

The utility model discloses an intermittently immersed opening-closing type plant bioreactor, which comprises an inflator pump connected with a time controller, a liquid storage chamber and a main reactor; the inflator pump is hermetically connected with an inlet of the liquid storage chamber through an air inlet pipe, and an air degerming device I is arranged on the air inlet pipe; the liquid storage chamber is isolated from the main reactor by a glass plate, the glass plate is provided with a glass bent pipe, and two ends of the glass bent pipe are respectively communicated with the liquid storage chamber and the main reactor. The intermittent immersion open-close type plant bioreactor has the characteristics of simple structure, low manufacturing cost and good actual use effect.

Description

Intermittent submerged open-close plant bioreactor

technical field

The utility model relates to a reactor for plant tissue culture, in particular to a culture device for intermittent submerged open-close plant tissue (such as callus, organ, hairy root, adventitious bud and seed embryo, etc.) .

Background technique

At present, in the field of plant tissue culture, the culture method mainly refers to solid or semi-solid culture in which agar is used as a support in a culture bottle. This culture method needs to consume a large amount of agar, and a large number of containers need to be handled when filling and cleaning, so it consumes a lot of labor, resulting in high production costs. Although the liquid culture method has the advantages of easy renewal, no need to change containers, convenient filter sterilization, convenient cleaning, convenient application of large containers, and reduced time for seedling transfer, etc.; however, the disadvantages of liquid culture are also relatively concentrated, such as easy hypoxia, long-term immersion in Plant organs in liquid are easy to vitrify, plant organs are sensitive to stirring force, and require complex equipment.

The research on plant tissue culture reactors mainly includes the following aspects: 1. Improvements to traditional stirred tanks and airlift tanks; however, the phenomenon of vitrification cannot be solved because submerged culture is still used. 2. Laminar flow reactors, such as shallow layer and drum culture reactors; although vitrification is reduced, the culture effect is not ideal. 3. Spraying and atomization reactors, the cultivating effect of such reactors is relatively ideal, and there are related patent applications, such as the "Plant Organ Biological Cultivation Reactor" with the patent No. Rotating sieve plate mist flow plant tissue culture reactor”, and the patent No. 96207831.X “elastic valve sieve plate plant tissue culture reactor”. However, with the growth of the plants in the later stage of the cultivation, the misty culture solution is difficult to distribute evenly, which seriously affects the growth of the plants. 4. Intermittent immersion reactor, this type of reactor mainly uses culture medium to cultivate plant tissue by intermittent immersion, supplies nutrients to plants during immersion, and provides enough oxygen to plants during intermittent periods. Because this culture method can better solve the problem of vitrification, and the nutrients can be well transferred along with the immersion of plant tissue; therefore, this culture method can well solve various problems in plant tissue culture . There are many studies on this kind of culture method abroad, and there are related patent applications for this kind of reactor in China, such as the "Airlift Periodic Immersion Illumination Plant Cell Tissue and Organ Culture Reactor" with the patent number 98205797.0, and the "Plant Cell Tissue and Organ Culture Reactor" with the patent number 98102396.7. "Air-lift type periodical immersion light plant cell tissue organ culture method and culture reactor", etc., are all difficult to popularize due to the complicated structure of the equipment and high manufacturing cost.

The patent No. 200920116327 "Cultivation Reactor for Plant Tissues and Organs Immersed Intermittently" is simpler in structure and lower in cost than the former two, but the opening of the main reaction tank of this equipment is small, and it is difficult to take out the regenerated plants. Even the main reaction tank needs to be destroyed to get seedlings; in addition, because the liquid storage tank and the main reaction tank are two independent components, it is inconvenient to carry and sterilize.

Utility model content

The technical problem to be solved by the utility model is to provide an intermittent immersion type plant bioreactor with simple structure, low manufacturing cost and good actual use effect.

In order to solve the above technical problems, the utility model provides an intermittent submerged open-close plant bioreactor, which includes an air pump connected with a time controller, and also includes a liquid storage chamber and a main reactor; The entrance of the chamber is sealed and connected, and an air sterilizer I is installed on the air intake pipe; the liquid storage chamber and the main reactor are separated by a glass plate, and a glass bend is arranged on the glass plate, and the two ends of the glass bend are respectively connected to the main reactor. The liquid storage chamber communicates with the main reactor.

As an improvement of the intermittent submerged open-close type plant bioreactor of the present invention: a vent hole is arranged at the center of the glass plate, and the glass elbow located in the cavity of the liquid storage chamber is connected to the vent hole in a sealed manner.

As a further improvement of the intermittent submerged open-close plant bioreactor of the present utility model: a filter screen is arranged in the glass elbow.

As a further improvement of the intermittent submerged open-close plant bioreactor of the present utility model: the liquid storage chamber is provided with an inlet pipe that is sealed and communicated with the cavity of the liquid storage chamber, and one end of the air sterilizer 1 is sealed and communicated with the inlet pipe , The other end is in communication with the air intake pipe.

As a further improvement of the intermittent submerged open-close plant bioreactor of the present invention: a liquid discharge port is provided at the bottom of the liquid storage chamber, and a sealing plug is provided in the liquid discharge port.

As a further improvement of the intermittent submerged open-close plant bioreactor of the present utility model: the main reactor is composed of a main reaction chamber and an openable cover, the cover is sealed and connected with the main reaction chamber, and the inner part of the main reaction chamber is arranged on the cover. The cavity is sealed and connected to the outlet pipe, and the outlet pipe is connected to the air sterilizer II in a sealed connection.

As a further improvement of the intermittent submerged split-type plant bioreactor of the present invention: the glass plate is arc-shaped, and the concave part of the glass plate faces the inner cavity of the main reaction chamber.

The intermittent submerged open-close type plant bioreactor of the utility model uses a time controller to control the on-off of the current, so as to realize whether the air pump is working or not. When the inflatable pump works, under the action of air pressure, the culture solution in the liquid storage chamber enters the main reactor, thereby infiltrating the plant tissue in the main reactor, and the gas in the main reactor can be discharged from the outlet pipe. When the air pump stops working, the culture solution in the main reactor returns to the liquid storage chamber under the action of gravity. In this way, the plant tissue in the main reactor can be submerged intermittently by the culture solution. The design of the open-close main reactor makes it easy to pick and place plant tissues, and the integrated design of the liquid storage chamber and the main reactor further simplifies the structure of the utility model, reduces the production cost, and facilitates large-scale production and promotional use.

Description of drawings

Below in conjunction with accompanying drawing, the specific embodiment of the present utility model is described in further detail.

Fig. 1 is a structural schematic diagram of an intermittent submerged open-close plant bioreactor of the present invention.

Detailed ways

Figure 1 shows a split-type plant bioreactor submerged intermittently, which mainly includes a time controller 1, an air pump 2, a liquid storage chamber 5 and a main reactor 7, etc., and the time controller 1 is connected to the air pump 2 through a wire , The work of the air pump 2 is controlled by the time controller 1.

The liquid storage chamber 5 is isolated from the main reactor 7 by a glass plate 6 , the glass plate 6 is arc-shaped, and the concave portion of the glass plate 6 faces the inner cavity of the main reactor 7 . The main reactor 7 is made up of a main reaction chamber 74 and an openable lid 73, and the lid 73 and the main reaction chamber 74 are sealed and connected by a frosted mouth 72 or a screw thread; that is, the recess of the glass plate 6 faces the main reaction chamber 74. The outlet pipe 71 that is sealed and communicated with the inner chamber of the main reactor 7 (i.e. the inner chamber of the main reaction chamber 74) is set on the cover 73, and the air sterilizer II8 outside the main reactor 7 is sealed and connected with the outlet pipe 71 Pass.

A vent hole 63 is set at the center of the glass plate 6, and the glass elbow 61 in the cavity of the liquid storage chamber 5 communicates with the vent hole 63 in a sealed manner; thereby realizing the liquid storage chamber 5 and the main reactor 7 (i.e. the main reactor The reaction chambers 74) are communicated by means of glass elbows 61. A filter screen 62 is arranged in the glass elbow 61 , and the purpose of the filter screen 62 is to prevent the plant tissue in the main reaction chamber 74 from falling into the liquid storage chamber 5 through the glass elbow 61 . Liquid discharge port 52 is set at the bottom of liquid storage chamber 5, and sealing plug 53 is set in liquid discharge port 52; . The side wall of the liquid storage chamber 5 is provided with an inlet pipe 51 that is sealed and communicated with the liquid storage chamber 5 inner cavity, and one end of the air sterilizer 14 outside the liquid storage chamber 5 is connected with the inlet pipe 51 seal, and the other One end is in sealing communication with the intake pipe 3 ; the air intake pipe 3 is in sealing communication with the air pump 2 .

The intermittent submerged open-close type plant bioreactor of the present utility model, actual work process is as follows:

1. Plug the sealing plug 53, open the cover 73, put an appropriate amount of culture solution into the main reaction chamber 74, the culture solution enters the liquid storage chamber 5 through the glass elbow 61, and cover the cover 73. Then the entire reactor except the timer 1 and the air pump 2 was sterilized by moist heat at 121° C. for 25 min.

2. Under aseptic conditions, open the cover 73, put the plant tissues and organs to be cultured into the main reaction chamber 74, and then close the cover 73 to keep the airtightness of the entire main reactor 7.

3. The air pump 2 works intermittently under the control of the time controller 1.

When the air pump 2 was in working order, the gas entered the intake pipe 3 and was sterilized by the air sterilizer 14, and then entered the inner cavity of the liquid storage chamber 5 through the inlet pipe 51; Under the action of the glass elbow 61 and the vent hole 63, it enters the main reaction chamber 74 successively, so that the plant tissues and organs in the main reaction chamber 74 are immersed in the culture solution. The gas in the main reaction chamber 74 is then discharged through the outlet pipe 71 and the air sterilizer II 8 successively.

When the inflator 2 stopped working, the culture solution in the main reaction chamber 74 would return to the liquid storage chamber 5 through the air vent 63 and the glass elbow 61 successively under the action of its own gravity; filter screen 62, so the plant tissues and organs in the main reaction chamber 74 will not flow back into the liquid storage chamber 5 along with the culture solution. At this time, a negative pressure is formed in the entire main reactor 7, and the outside gas enters the main reaction chamber 74 along the outlet pipe 71 after being sterilized by the air sterilizer II8.

Like this, the plant tissues and organs in the main reaction chamber 74 have just completed a cycle that is submerged intermittently. The time of the above-mentioned cycle can be adjusted by adjusting the time controller 1.

4. After the cultivation, under aseptic conditions, open the sealing plug 53 to discharge the culture medium, then open the cover 73 to take out the regenerated plants.

Finally, it should also be noted that what is listed above is only a specific embodiment of the present invention. Apparently, the utility model is not limited to the above embodiments, and many variations are possible. All deformations that a person skilled in the art can derive or associate directly from the content disclosed in the utility model shall be considered as the protection scope of the utility model.

Claims (7)

1. The split-type plant bioreactor submerged intermittently is characterized in that it includes an air pump (2) connected to the time controller (1), and also includes a liquid storage chamber (5) and a main reactor (7); the air pump (2) connect to each other with the inlet seal of liquid storage chamber (5) by air inlet pipe (3), air sterilizer I (4) is set on air inlet pipe (3); Liquid storage chamber (5) and main reactor (7) ) are separated by a glass plate (6), and a glass bend (61) is arranged on the glass plate (6). Device (7) is connected. 2. The split-type plant bioreactor submerged intermittently according to claim 1, characterized in that: a ventilation hole (63) is set at the center of the glass plate (6), located in the cavity of the liquid storage chamber (5) The inner glass elbow (61) is in communication with the vent hole (63) in a sealed manner. 3. The intermittent submerged open-close plant bioreactor according to claim 2, characterized in that: a filter screen (62) is arranged in the glass elbow (61). 4. The intermittent submerged open-close plant bioreactor according to claim 3, characterized in that: the liquid storage chamber (5) is provided with an inlet pipe (51) that is in communication with the liquid storage chamber (5) inner cavity ), one end of the air sterilizer I (4) is sealed and connected with the inlet pipe (51), and the other end is connected with the air inlet pipe (3) sealed. 5. The intermittent submerged open-close plant bioreactor according to claim 4, characterized in that: a liquid discharge port (52) is set at the bottom of the liquid storage chamber (5), and in the liquid discharge port (52) Set the sealing plug (53). 6. The open-close type plant bioreactor submerged intermittently according to claim 5, characterized in that: the main reactor (7) is made up of a main reaction chamber (74) and an openable cover (73), the The cover (73) is sealed and connected with the main reaction chamber (74), and the outlet pipe (71) connected with the inner cavity of the main reaction chamber (74) is arranged on the cover (73), and the outlet pipe (71) is connected with the air for sterilization Device II (8) is sealed and communicated. 7. The intermittent submerged open-close plant bioreactor according to claim 6, characterized in that: the glass plate (6) is arc-shaped, and the concave portion of the glass plate (6) faces the main reaction chamber (74) lumen.