CN201809355U - Intermittent-immersion openable plant bioreactor - Google Patents
Intermittent-immersion openable plant bioreactor Download PDFInfo
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- CN201809355U CN201809355U CN 201020288879 CN201020288879U CN201809355U CN 201809355 U CN201809355 U CN 201809355U CN 201020288879 CN201020288879 CN 201020288879 CN 201020288879 U CN201020288879 U CN 201020288879U CN 201809355 U CN201809355 U CN 201809355U
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- fluid reservoir
- close type
- type plant
- plant bioreactor
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Abstract
The utility model discloses an intermittent-immersion openable plant bioreactor, which comprises an inflating pump, a liquid storage chamber and a main reactor. The inflating pump is connected with a time controller, and is connected with an inlet of the liquid storage chamber via an air inlet pipe hermetically, an air sterilizer I is arranged on the air inlet pipe, the liquid storage chamber is isolated from the main reactor via a glass sheet, a glass bent pipe is arranged on the glass sheet, and two ends of the glass bent pipe are respectively communicated with the liquid storage chamber and the main reactor. The intermittent-immersion openable plant bioreactor has the advantages of simple structure, low manufacturing cost and fine actual using effect.
Description
Technical field
The utility model relates to a kind of reactor that is used for plant tissue culture, specifically is a kind of intermittently culture apparatus of buried open-close type plant tissue (for example callus, organ, hairly root, indefinite bud and embryo etc.) that is used for.
Background technology
In the plant tissue culture field, training method mainly is meant in culturing bottle solid or the semisolid cultivation of agar as upholder at present.This training method need expend a large amount of agar, and need handle a large amount of containers when can and cleaning, therefore expends a large amount of labor forces, thereby causes production cost high.Though the liquid culture mode have upgrade easily, need not to change container, make things convenient for filtration sterilization, clean convenient, be convenient to tun and use, reduce advantages such as changeing the seedling time; But shortcoming is also more concentrated when liquid culture, for example easily anoxic, for a long time be immersed in the easy vitrifying of plant organ in the liquid, plant organ is to the whipping force sensitivity and need complex apparatus etc.
Research for the plant tissue culture reactor mainly contains following aspect: 1, to the improvement of conventional stirred-tank, gas lift jar; But owing to still adopt immersion to cultivate, so vitrification phenomenon can't solve.2, course flow reactor is cultivated reactor as shallow-layer formula and rotary drum; Though reduced vitrification phenomenon, but culture effect is undesirable.3, spray, atomizing type reactor, this type of reactor culture effect is comparatively desirable, relevant patent application is also arranged now, as the patent No. is that 97220500.4 " plant organ biological culture reactor " and the patent No. are 96204509.8 " the board-like spray flow plant tissue culture of multilayer trundle screen reactor ", and the patent No. is 96207831.X " a feather valve sieve plate plant tissue culture reactor ".But this kind training method is in the growth of late stage of culture along with plant, and vaporific nutrient solution is difficult to be evenly distributed, and has had a strong impact on the growth of plant.4, submergence at intermittence reactor, this type of reactor mainly is to utilize nutrient solution that the submergence at intermittence of plant tissue is cultivated, and supplies with plant nutrition during submergence, provides plant enough oxygen again in the time of intermittently.Because this training method preferably resolves vitrified problem, and nutritive substance is along with the submergence to plant tissue also can well be transmitted; Therefore this training method can well solve the various difficult problems in the plant tissue culture.This type of training method foreign study is more, and this kind reactor also has relevant patent application at home, as the patent No. 98205797.0 " Airlift cyclic immersion lighting plant cell tissue organ cultivation reactor ", the patent No. is 98102396.7 " Airlift cyclic immersion lighting plant cell tissue organ cultural method and a cultivation reactor " etc., all, cause being difficult for popularizing because of complex structure, the manufacturing cost height of equipment.
The patent No. is 200920116327 " the intermittently cultivation reactor of submergence plant tissue organ " though more simple and easy compared to the above two structures, cost is also comparatively cheap, but the main treatment tank opening of this equipment is less, regeneration plant be difficult for to take out, sometimes even need to destroy main treatment tank and just can get seedling; In addition, because container for storing liquid and main treatment tank are two independent components, give and carry and sterilization is made troubles.
The utility model content
The technical problems to be solved in the utility model provides a kind of simple in structure, cheap for manufacturing cost, buried open-close type plant bioreactor at intermittence that practical effect is good.
In order to solve the problems of the technologies described above, the utility model provide a kind of intermittence buried open-close type plant bioreactor, comprise the aerator pump that links to each other with time controller, also comprise fluid reservoir and main reactor; Aerator pump links to each other with the inlet seal of fluid reservoir by inlet pipe, and air sterilization device I is set on inlet pipe; Rely on sheet glass isolated between fluid reservoir and the main reactor, be provided with the glass bend pipe on sheet glass, the two ends of glass bend pipe are connected with fluid reservoir and main reactor respectively.
Improvement as buried open-close type plant bioreactor at intermittence of the present utility model: in the center of sheet glass a breeder tube is set, the glass bend pipe that is positioned at the fluid reservoir cavity is connected hermetically with breeder tube.
Further improvement as buried open-close type plant bioreactor at intermittence of the present utility model: in the glass bend pipe, filtering net is set.
Further improvement as buried open-close type plant bioreactor at intermittence of the present utility model: be provided with the inlet pipe that is connected with the fluid reservoir bore seal on fluid reservoir, the end of air sterilization device I is connected with the inlet pipe sealing, the other end seals with inlet pipe and is connected.
Further improvement as buried open-close type plant bioreactor at intermittence of the present utility model: the bottom at fluid reservoir is provided with tap hole, and sealing plug is set in tap hole.
Further improvement as buried open-close type plant bioreactor at intermittence of the present utility model: main reactor is made up of main reaction chamber and openable lid, lid links to each other with the main reaction chamber sealing, the outlet pipe that is connected with the bore seal of main reaction chamber is set on lid, and outlet pipe is connected with air sterilization device II sealing.
Further improvement as buried open-close type plant bioreactor at intermittence of the present utility model: the sheet glass camber, the recess of sheet glass faces the inner chamber of main reaction chamber.
Intermittence of the present utility model, buried open-close type plant bioreactor utilized time controller to come the break-make of control current, thereby the work that realizes the control aerator pump whether.When aerator pump was worked, under the effect of air pressure, the nutrient solution in the fluid reservoir entered in the main reactor, thereby soaked into the plant tissue in the main reactor, and the gas in the main reactor can be discharged from from outlet pipe.When aerator pump quit work, the nutrient solution in the main reactor was back to fluid reservoir under the effect of gravity.Like this, just can realize that the interior plant tissue of main reactor is by intermittently submergence of nutrient solution.The design of the main reactor of open-close type makes picking and placeing of plant tissue become convenient and easy, and structure of the present utility model has further been simplified in the integration of fluid reservoir and main reactor design, has reduced cost of manufacture, is convenient to large-scale production and promotes the use of.
Description of drawings
Below in conjunction with accompanying drawing embodiment of the present utility model is described in further detail.
Fig. 1 is the structural representation of buried open-close type plant bioreactor at intermittence of the present utility model.
Embodiment
Fig. 1 has provided buried open-close type plant bioreactor at a kind of intermittence, mainly comprises time controller 1, aerator pump 2, fluid reservoir 5 and main reactor 7 etc., and time controller 1 links to each other with aerator pump 2 by lead, and whether the work of aerator pump 2 is controlled by time controller 1.
Rely on sheet glass 6 isolated between fluid reservoir 5 and the main reactor 7, sheet glass 6 cambers, the recess of sheet glass 6 faces the inner chamber of main reactor 7.Main reactor 7 is made up of main reaction chamber 74 and openable lid 73, revolves cause for gossip by frosted mouth 72 or screw thread between lid 73 and the main reaction chamber 74 and now seals and link to each other; That is, the recess of sheet glass 6 faces main reaction chamber 74.The outlet pipe 71 that is connected with inner chamber (being the inner chamber of the main reaction chamber 74) sealing of main reactor 7 is set on lid 73, is positioned at main reactor 7 outer air sterilization device II 8 and is connected with outlet pipe 71 sealings.
In sheet glass 6 centers one breeder tube 63 is set, the glass bend pipe 61 that is positioned at fluid reservoir 5 cavitys is connected hermetically with breeder tube 63; Thereby realize that dependence glass bend pipe 61 is connected between fluid reservoir 5 and the main reactor 7 (being main reaction chamber 74).Filtering net 62 is set in glass bend pipe 61, and the purpose that filtering net 62 is set is: fall in the fluid reservoir 5 by glass bend pipe 61 in order to prevent the plant tissue in the main reaction chamber 74.In the bottom of fluid reservoir 5 tap hole 52 is set, sealing plug 53 is set in tap hole 52; Experiment is extracted sealing plug 53 after finishing, can be from the liquid nutrient medium in the tap hole 52 discharge fluid reservoirs 5.Be provided with the inlet pipe 51 that is connected with fluid reservoir 5 bore seal on the sidewall of fluid reservoir 5, an end that is positioned at the air sterilization device I 4 outside the fluid reservoir 5 is connected with inlet pipe 51 sealings, the other end is connected with inlet pipe 3 sealings; Inlet pipe 3 is connected with aerator pump 2 sealings.
Intermittence of the present utility model buried open-close type plant bioreactor, practical work process is as follows:
1, sealing plug 53 beyond the Great Wall, uncap 73 is put into main reaction chamber 74 with an amount of nutrient solution, and nutrient solution enters in the fluid reservoir 5 by glass bend pipe 61, cover lid 73.To under 121 ℃, carry out the moist heat sterilization of 25min except the whole reactor time controller 1 and the aerator pump 2 then.
2, under aseptic condition, uncap 73, plant tissue organ to be cultivated put into main reaction chamber 74 after, close the cover 73 keeps whole main reactor 7 stopping propertys.
3, aerator pump 2 carries out discontinuous operation under the control of time controller 1.
When aerator pump 2 was in running order, gas entered inlet pipe 3 and carries out degerming by air sterilization device I 4 and handle, and enters fluid reservoir 5 inner chambers by inlet pipe 51 then; Nutrient solution in the fluid reservoir 5 enters in the main reaction chamber 74 by glass bend pipe 61 and breeder tube 63 under the effect of air pressure successively, and the plant tissue organ in the main reaction chamber 74 is immersed in the nutrient solution.Gas in the main reaction chamber 74 then is discharged from by outlet pipe 71 and air sterilization device II 8 successively.
When aerator pump 2 quit work, the nutrient solution in the main reaction chamber 74 was back in the fluid reservoir 5 by breeder tube 63 and glass bend pipe 61 under the effect of self gravitation successively; Owing to be provided with filtering net 62 in the glass bend pipe 61, so the plant tissue organ in the main reaction chamber 74 can be along with nutrient solution is back in the fluid reservoir 5.Form negative pressure in the whole main reactor 7 this moment, enters in the main reaction chamber 74 along outlet pipe 71 after the germicidal treatment of extraneous gas through air sterilization device II8.
Like this, the plant tissue organ in the main reaction chamber 74 has just been finished one by intermittently buried circulation.Can regulate the above-mentioned round-robin time by regulating time controller 1.
4, cultivate the end back under aseptic condition, open sealing plug 53, discharge substratum, uncap 73 again, take out regeneration plant.
At last, it is also to be noted that what more than enumerate only is a specific embodiment of the present utility model.Obviously, the utility model is not limited to above embodiment, and many distortion can also be arranged.All distortion that those of ordinary skill in the art can directly derive or associate from the disclosed content of the utility model all should be thought protection domain of the present utility model.
Claims (7)
1. buried open-close type plant bioreactor intermittently is characterized in that: comprise the aerator pump (2) that links to each other with time controller (1), also comprise fluid reservoir (5) and main reactor (7); Aerator pump (2) links to each other with the inlet seal of fluid reservoir (5) by inlet pipe (3), and air sterilization device I (4) is set on inlet pipe (3); Rely on sheet glass (6) isolated between fluid reservoir (5) and the main reactor (7), be provided with glass bend pipe (61) on sheet glass (6), the two ends of described glass bend pipe (61) are connected with fluid reservoir (5) and main reactor (7) respectively.
2. intermittence according to claim 1 buried open-close type plant bioreactor, it is characterized in that: in the center of sheet glass (6) breeder tube (63) is set, the glass bend pipe (61) that is positioned at fluid reservoir (5) cavity is connected hermetically with breeder tube (63).
3. intermittence according to claim 2, buried open-close type plant bioreactor is characterized in that: filtering net (62) is set in described glass bend pipe (61).
4. intermittence according to claim 3 buried open-close type plant bioreactor, it is characterized in that: be provided with the inlet pipe (51) that is connected with fluid reservoir (5) bore seal on fluid reservoir (5), the end of air sterilization device I (4) is connected with inlet pipe (51) sealing, the other end is connected with inlet pipe (3) sealing.
5. intermittence according to claim 4, buried open-close type plant bioreactor is characterized in that: in the bottom of fluid reservoir (5) tap hole (52) is set, sealing plug (53) is set in described tap hole (52).
6. intermittence according to claim 5 buried open-close type plant bioreactor, it is characterized in that: described main reactor (7) is made up of main reaction chamber (74) and openable lid (73), described lid (73) links to each other with main reaction chamber (74) sealing, go up the outlet pipe (71) that setting is connected with the bore seal of main reaction chamber (74) at lid (73), outlet pipe (71) is connected with air sterilization device II (8) sealing.
7. intermittence according to claim 6 buried open-close type plant bioreactor, it is characterized in that: described sheet glass (6) camber, the recess of sheet glass (6) faces the inner chamber of main reaction chamber (74).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN 201020288879 CN201809355U (en) | 2010-08-10 | 2010-08-10 | Intermittent-immersion openable plant bioreactor |
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| Application Number | Priority Date | Filing Date | Title |
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| CN 201020288879 CN201809355U (en) | 2010-08-10 | 2010-08-10 | Intermittent-immersion openable plant bioreactor |
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| CN201809355U true CN201809355U (en) | 2011-04-27 |
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| CN 201020288879 Expired - Fee Related CN201809355U (en) | 2010-08-10 | 2010-08-10 | Intermittent-immersion openable plant bioreactor |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102150624A (en) * | 2011-04-29 | 2011-08-17 | 南京工业大学 | Tissue culture and rapid propagation method for pinellia tuber plant |
| CN103120125A (en) * | 2013-02-04 | 2013-05-29 | 南京工业大学 | Method and device for high throughput screening of salt-tolerant plants |
| CN103999777A (en) * | 2013-02-04 | 2014-08-27 | 南京工业大学大丰海洋产业研究院 | Method for continuously obtaining pinellia ternata seedling secondary metabolites through tissue culture |
| CN104026019A (en) * | 2013-02-04 | 2014-09-10 | 南京工业大学大丰海洋产业研究院 | Method for continuously obtaining secondary ginseng hairy root metabolites through tissue culture |
| CN105104191A (en) * | 2015-08-11 | 2015-12-02 | 湖南科技学院 | Tissue culture reactor |
| WO2016172998A1 (en) * | 2015-04-30 | 2016-11-03 | 南京博方生物科技有限公司 | Plant bioreactor and use method therefor |
| CN111011212A (en) * | 2019-12-27 | 2020-04-17 | 南京师范大学 | Intermittent spraying type plant bioreactor and culture method |
-
2010
- 2010-08-10 CN CN 201020288879 patent/CN201809355U/en not_active Expired - Fee Related
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102150624A (en) * | 2011-04-29 | 2011-08-17 | 南京工业大学 | Tissue culture and rapid propagation method for pinellia tuber plant |
| CN102150624B (en) * | 2011-04-29 | 2013-05-15 | 南京工业大学 | Tissue culture and rapid propagation method for pinellia tuber plant |
| CN103120125A (en) * | 2013-02-04 | 2013-05-29 | 南京工业大学 | Method and device for high throughput screening of salt-tolerant plants |
| CN103999777A (en) * | 2013-02-04 | 2014-08-27 | 南京工业大学大丰海洋产业研究院 | Method for continuously obtaining pinellia ternata seedling secondary metabolites through tissue culture |
| CN104026019A (en) * | 2013-02-04 | 2014-09-10 | 南京工业大学大丰海洋产业研究院 | Method for continuously obtaining secondary ginseng hairy root metabolites through tissue culture |
| CN103999777B (en) * | 2013-02-04 | 2017-03-08 | 南京工业大学大丰海洋产业研究院 | The method that Rhizoma Pinelliae seedling secondary metabolite is persistently obtained by tissue culture |
| WO2016172998A1 (en) * | 2015-04-30 | 2016-11-03 | 南京博方生物科技有限公司 | Plant bioreactor and use method therefor |
| CN105104191A (en) * | 2015-08-11 | 2015-12-02 | 湖南科技学院 | Tissue culture reactor |
| CN105104191B (en) * | 2015-08-11 | 2017-07-14 | 湖南科技学院 | A kind of tissue cultures reactor |
| CN111011212A (en) * | 2019-12-27 | 2020-04-17 | 南京师范大学 | Intermittent spraying type plant bioreactor and culture method |
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Legal Events
| Date | Code | Title | Description |
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20110427 Termination date: 20110810 |