CN203373351U - Programmed domesticating and screening culture equipment - Google Patents
Programmed domesticating and screening culture equipment Download PDFInfo
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- CN203373351U CN203373351U CN201320461836.2U CN201320461836U CN203373351U CN 203373351 U CN203373351 U CN 203373351U CN 201320461836 U CN201320461836 U CN 201320461836U CN 203373351 U CN203373351 U CN 203373351U
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- 238000012216 screening Methods 0.000 title claims abstract description 23
- 239000002994 raw material Substances 0.000 claims abstract description 29
- 238000003860 storage Methods 0.000 claims abstract description 29
- 230000002572 peristaltic effect Effects 0.000 claims abstract description 19
- 238000012544 monitoring process Methods 0.000 claims abstract description 10
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract 5
- 239000001963 growth medium Substances 0.000 claims description 14
- 239000007788 liquid Substances 0.000 claims description 5
- 239000002609 medium Substances 0.000 claims description 4
- 238000002474 experimental method Methods 0.000 abstract description 12
- 238000000034 method Methods 0.000 abstract description 9
- 238000011160 research Methods 0.000 abstract description 2
- 230000000813 microbial effect Effects 0.000 abstract 1
- 238000012258 culturing Methods 0.000 description 31
- 238000012163 sequencing technique Methods 0.000 description 9
- 241000894006 Bacteria Species 0.000 description 4
- 238000003760 magnetic stirring Methods 0.000 description 4
- 238000013019 agitation Methods 0.000 description 3
- 244000005700 microbiome Species 0.000 description 3
- 241000233866 Fungi Species 0.000 description 2
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000011534 incubation Methods 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 238000003756 stirring Methods 0.000 description 2
- 239000002028 Biomass Substances 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000003044 adaptive effect Effects 0.000 description 1
- 239000003242 anti bacterial agent Substances 0.000 description 1
- 229940088710 antibiotic agent Drugs 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 238000012365 batch cultivation Methods 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 239000000356 contaminant Substances 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- 230000003252 repetitive effect Effects 0.000 description 1
- 230000000630 rising effect Effects 0.000 description 1
- 238000011146 sterile filtration Methods 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 238000004114 suspension culture Methods 0.000 description 1
- 238000012549 training Methods 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
- 238000002255 vaccination Methods 0.000 description 1
- 238000009423 ventilation Methods 0.000 description 1
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Abstract
本实用新型涉及一种程序化控制的驯化筛选培养设备，其包括：中央控制系统与若干执行单元；一个培养条件控制单元；一组培养瓶单元；一组原料储罐及培养物收集罐单元。所述中央控制系统与若干执行单元具有程序编辑与控制模块以及若干个蠕动泵；所述培养条件控制单元具有一个培养温度控制的循环水槽和一组磁力搅拌器；所述培养瓶单元包括带刻度的瓶体、瓶盖及与之相连的管线及监测部件，所述培养瓶单元放置于所述循环水槽中，所述磁力搅拌器转子置于所述培养瓶中，所述培养瓶通过其管路与所述蠕动泵连接；所述原料储罐与培养物收集罐单元具有若干个培养基原料储罐及培养物排出液收集罐，所述培养瓶通过其管路与所述培养基原料储罐及培养物排出液收集罐相连接。本实用新型具有自动化程序控制，培养体积小，可并行运行多种微生物驯化筛选培养条件，使用方便等优点，能够用来进行细菌、酵母、真菌等微生物及可悬浮培养细胞的驯化筛选培养工作，适用于生物、医药、农业等科研或工厂实验室中，并用来替代目前常用的传统的人工驯化筛选培养实验工作，节省人力成本并实现实验过程的标准化高效运行。
The utility model relates to a program-controlled domestication screening cultivation equipment, which comprises: a central control system and several execution units; a cultivation condition control unit; a group of culture bottle units; a group of raw material storage tanks and culture collection tank units. The central control system and several execution units have program editing and control modules and several peristaltic pumps; the culture condition control unit has a culture temperature-controlled circulating water tank and a set of magnetic stirrers; the culture bottle unit includes a graduated The bottle body, bottle cap, pipelines and monitoring components connected thereto, the culture bottle unit is placed in the circulating water tank, the magnetic stirrer rotor is placed in the culture bottle, and the culture bottle passes through its tube The road is connected with the peristaltic pump; the raw material storage tank and the culture collection tank unit have several medium raw material storage tanks and culture effluent collection tanks, and the culture bottle is connected to the medium raw material storage tank through its pipeline. The tank is connected with the culture effluent collection tank. The utility model has the advantages of automatic program control, small culture volume, multiple microbial domestication and screening culture conditions that can be run in parallel, and is convenient to use. It is suitable for biological, pharmaceutical, agricultural and other scientific research or factory laboratories, and is used to replace the traditional artificial domestication, screening and cultivation experiments commonly used at present, saving labor costs and achieving standardized and efficient operation of the experimental process.
Description
Technical field
But the utility model relates to a kind of sequencing that is specifically designed to the growths such as the microorganisms such as bacterium, yeast, fungi and suspension culture animal and plant cells, production performance improvement controls the acclimation and screening culture device.
Technical background
In microorganism or cell culture experiments, adaptive evolution (usually claiming " domestication ") is used to improve the production performance of cell or improves its physiological character as acid and alkali-resistance, anti-osmotic pressure, antibiotics resistant and organic solvent etc.Current experiment evolution adopts the perseveranceization cultivation usually and the two kinds of modes of cultivation that in batches go down to posterity are carried out, and wherein to go down to posterity in batches, cultivates more extensively employing.Traditional perseverance cultivation exists volume of culture large, the problem such as consume to cultivate base unit weight greatly and the sustainable time is short, and cause being difficult to carry out a plurality of parallel experiments because of reasons such as cost and controls.And traditional going down to posterity cultivated by manually carrying out in batches, the selection of every batch cultivation time and the incubation times that continues to go down to posterity have been subject to certain restriction, and the error probability that artificial repetitive operation brings and randomness, rising, cause the repeatability of this process poor.And repeatedly inoculation operation of existence is cultivated in batches going down to posterity of manually carrying out, and causes the risk of its pollution microbes greatly to improve.Therefore all can not meet well user demand to these traditional two kinds of experiment methods.
Summary of the invention
For the problems referred to above, the acclimation and screening culture device that provides a kind of programmable to control is provided main purpose of the present utility model, and it is low that it has equipment cost, but automatic routine are controlled, volume of culture is little, but the multiple acclimation and screening culture condition of parallel running, the advantage such as easy to use.
For achieving the above object, the acclimation and screening culture device that a kind of sequencing provided by the utility model is controlled, is characterized in that comprising: master control system and some actuator units; A culture condition control unit; One group of culturing bottle unit; One group of raw material storage tank and culture collection tank unit.Described master control system and some actuator units have program editing and control module and several peristaltic pumps; Described culture condition control unit have circulating water channel that culture temperature controls and one group of magnetic stirring apparatus; Described culturing bottle unit comprises bottle, bottle cap and the pipeline be attached thereto and the monitoring component with scale, described culturing bottle unit is positioned in described circulating water channel, described magnetic stirring apparatus rotor is placed in described culturing bottle, and described culturing bottle is connected with described peristaltic pump by its pipeline; Described raw material storage tank and culture collection tank unit have several culture medium raw material storage tanks and culture liquid effluent holding tank, and described culturing bottle is connected with described culture medium raw material storage tank and culture liquid effluent holding tank by its pipeline.
In above-mentioned the technical solution of the utility model, the master control system of the acclimation and screening culture device that described sequencing is controlled is arranged in a frame with some actuator units are distributed, and program editing and control module are moved under setting program by the time variable control peristaltic pump of editing and running package contains some experiment parameters.
The culture condition control unit of the acclimation and screening culture device that described sequencing is controlled is placed on master control system and actuator unit frame the place ahead, described magnetic stirring apparatus, is positioned over circulating water channel inside or below.
The culturing bottle unit of the acclimation and screening culture device that described sequencing is controlled comprises bottle, bottle cap and the pipeline be attached thereto and the monitoring component with scale, described culturing bottle unit is positioned in described circulating water channel, described magnetic stirring apparatus rotor is placed in described culturing bottle, and described culturing bottle is connected with described peristaltic pump by its pipeline.
Raw material storage tank and the culture collection tank unit of the acclimation and screening culture device that described sequencing is controlled have several culture medium raw material storage tanks and culture liquid effluent holding tank, and described culturing bottle is connected with described culture medium raw material storage tank and culture liquid effluent holding tank by its pipeline.
Adopt technique scheme, the characteristics that the utlity model has are: (1) controls the operation of peristaltic pump by editor, realizes the quantitative interpolation of culturing bottle unit substratum and quantitatively shifting out of nutrient solution; The continuous and automatic of cultivating of realizing in batches going down to posterity carries out; (2), by the operation of time variable control peristaltic pump and the combination of culture medium raw material storage tank different culture media composition, realize the parallel cultivation operation of multiple different culture media condition; (3) operation that realizes different vaccination ratio culture condition by operation and the culturing bottle unit of time variable control peristaltic pump; (4) by the execution of time variable control and culture condition control unit, realize the operation of the culture condition such as differing temps and stirring; (5) monitoring component be equipped with by the culturing bottle unit is realized the record of the key state parameter of culturing process.The utlity model has automatic routine controls, volume of culture is little, but parallel running multiple-microorganism or cell acclimation and screening culture condition, the advantage such as easy to use, but can be used for carrying out the acclimation and screening training of the microorganisms such as bacterium, yeast, fungi and suspended culture cell, be applicable to biology,, medicine, agricultural, etc. in scientific research or plant laboratory, and be used for substituting traditional domestication's screening and culturing cut-and-try work commonly used at present, save human cost and realize that the stdn of experimentation efficiently moves.
Concrete enforcement
Now for embodiment once and by reference to the accompanying drawings structure of the present utility model and effect are elaborated.
As shown in Figures 1 to 3, the acclimation and screening culture device that sequencing provided by the utility model is controlled comprises master control system and actuator unit 1; Culture condition control unit 2; Culturing bottle unit 3 and raw material storage tank and culture collection tank unit 4.Master control system and actuator unit 1 are comprised of program editing and control module 10 and at least one peristaltic pump group 11 and 12; Culture condition control unit 2 is consisted of the circulator bath with temperature control modules 22 and circulating water channel 20 and magnetic agitation system 21; Has one group of culturing bottle unit 3 at least in circulator bath 20; Have at least one group of raw material storage tank and culture collection tank unit 4 to be connected with culturing bottle unit 3.
As Fig. 1, shown in 2, master control system and actuator unit 1 have a frame 13, are equipped with at least one peristaltic pump group 11 and 12 on frame; Master control system and actuator unit 1 have a set of program editing and control module 10; Program editing is connected with 12 and controls its operation with peristaltic pump group 11 by signal wire with control module 10.
As shown in Figure 3, culture condition control unit 2 is consisted of the circulator bath with temperature control modules 22 and circulating water channel 20 and magnetic agitation system 21; Temperature control modules 22 is positioned at a jiao of circulating water channel 20, and magnetic agitation system 21 is positioned at below or the inside of circulating water channel 20.
As shown in Figure 4, culturing bottle unit 3 has bottle 31 and the bottle cap thereof with scale, and bottle cap has pipe connecting 32,33, and 34,35 and detection part 36 interfaces; Pipe connecting 32,33,34,35 have ventilation, and the access substratum, shift out nutrient solution, the parts effects such as exhaust and the monitoring of installation biomass; Pipe connecting has pipe clamp and steps up pipeline.
As shown in Figure 5: raw material storage tank and culture collection tank unit 4 have at least one group of raw material storage tank 40 and culture collection tank 41; Raw material storage tank 40 has a substratum and shifts out pipe connecting 401 and air pipe connecting 402; And culture collection tank 41 has a culture collection pipe connecting 411 and air pipe connecting 402.
Working process of the present utility model is: (1) adds appropriate experiment substratum 37 in culturing bottle unit 3, puts into magnetic stir bar 30 and builds bottle cap, steps up respective line with pipe clamp simultaneously; Then the medium feed pipe connecting is connected with culture medium raw material storage tank 40; Install the substratum of experiment use in culture medium raw material storage tank 40, build lid together with corresponding culturing bottle unit 3, culture collection tank 41 is high-temperature sterilization together; (2) until culture medium raw material storage tank 40 and corresponding culturing bottle unit 3 thereof gone out bacterium and cooling after, in aseptic operating platform, in the culturing bottle unit, inoculate into the bacterial classification that will carry out the acclimation and screening cultivation; (3) will the go out culture medium raw material storage tank 40 of bacterium is placed on a side of circulating water channel 20, and vaccinated culturing bottle unit 3 that will be corresponding with it is placed in circulating water channel 20; The pipe connecting that culturing bottle unit 3 is connected with culture medium raw material storage tank 40 is installed in peristaltic pump group 11, culture is shifted out to pipe connecting and be installed in peristaltic pump group 12; Need to connect the sterile filtration air according to culture condition, (4) such as culturing process parameter monitoring parts set culture temperature, rotating speed, the culture transferring ratio, the substratum blending ratio, start operation after the parameters such as single culture cycle incubation time, the key state parameter in the monitoring component that can be connected and installed monitoring culturing process simultaneously.
Adopt said structure, the utlity model has following advantage:
1. the utility model can be realized traditional automatic routine controlling run that goes down to posterity in batches and cultivate; Replace artificial experiment to save human cost, and have easy to use, safeguard simple, many-sided advantage such as anti-living contaminants.
2. the utility model can carry out a plurality of parallel experiment evolution work simultaneously, and can control realize kinds of experiments evolution conditions time operation by sequencing, has improved the efficiency that experiment is evolved.
3. the utility model culturing bottle unit culture system is little, and sustainable long-time steady operation simultaneously can record key state parameter in culturing process, for trackability and the repeatability of experimentation, has meaning.
The above, be only the preferred embodiment of this practical habit, and all according to the utility model specification sheets, the equivalent transformation that claims and accompanying drawing are done, all should be included in scope of patent protection of the present utility model.
The accompanying drawing explanation:
Fig. 1 is the structural representation of the utility model one preferred embodiment;
The master control system of Fig. 2 the utility model one preferred embodiment and the structural representation of actuator unit;
The structural representation of the culture condition control unit of Fig. 3 the utility model one preferred embodiment;
The structural representation of the culturing bottle unit of Fig. 4 the utility model one preferred embodiment;
The structural representation of the culture medium raw material storage tank of Fig. 5 the utility model one preferred embodiment and culture collection tank unit
Claims (5)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201320461836.2U CN203373351U (en) | 2013-07-27 | 2013-07-27 | Programmed domesticating and screening culture equipment |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201320461836.2U CN203373351U (en) | 2013-07-27 | 2013-07-27 | Programmed domesticating and screening culture equipment |
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| Publication Number | Publication Date |
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| CN203373351U true CN203373351U (en) | 2014-01-01 |
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| CN201320461836.2U Expired - Lifetime CN203373351U (en) | 2013-07-27 | 2013-07-27 | Programmed domesticating and screening culture equipment |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107603898A (en) * | 2017-10-26 | 2018-01-19 | 中国科学院天津工业生物技术研究所 | Saccharomyces cerevisiae and its breeding methods and the application of industrial fermentation to produce ethanol |
| CN111269822A (en) * | 2018-12-05 | 2020-06-12 | 广州中国科学院先进技术研究所 | A multifunctional high-throughput directed evolution system and directed evolution method |
| CN113897274A (en) * | 2021-10-21 | 2022-01-07 | 九江学院 | Microorganism enrichment culture apparatus |
-
2013
- 2013-07-27 CN CN201320461836.2U patent/CN203373351U/en not_active Expired - Lifetime
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107603898A (en) * | 2017-10-26 | 2018-01-19 | 中国科学院天津工业生物技术研究所 | Saccharomyces cerevisiae and its breeding methods and the application of industrial fermentation to produce ethanol |
| CN107603898B (en) * | 2017-10-26 | 2019-06-28 | 中国科学院天津工业生物技术研究所 | Saccharomyces cerevisiae and its breeding methods and the application of industrial fermentation to produce ethanol |
| CN111269822A (en) * | 2018-12-05 | 2020-06-12 | 广州中国科学院先进技术研究所 | A multifunctional high-throughput directed evolution system and directed evolution method |
| CN113897274A (en) * | 2021-10-21 | 2022-01-07 | 九江学院 | Microorganism enrichment culture apparatus |
| CN113897274B (en) * | 2021-10-21 | 2022-05-06 | 九江学院 | Microorganism enrichment culture apparatus |
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| CX01 | Expiry of patent term |