CN108485941A - A kind of photosynthetic bacteria cultural method - Google Patents
A kind of photosynthetic bacteria cultural method Download PDFInfo
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Abstract
The invention belongs to Bacteria Culture technical fields, specifically a kind of photosynthetic bacteria cultural method, the process employs photosynthetic bacterias to cultivate instrument, wherein culture instrument includes glass jar, luminescent screen, power generation plate, acid-base solution case, motor, rotation axis, stirring module, acid-base accommodation module, rotating disc, motor one, inlet port and outlet port, and glass jar is for containing culture medium solution and photosynthetic bacteria；Luminescent screen is concentrically mounted on the outside of glass jar；Acid-base solution case is located at the top of cover；Motor is located at the top of soda acid solvent tank；Stirring module is for stirring photosynthetic bacteria；Acid-base accommodation module is mounted on the upper end of rotation axis, and acid-base accommodation module is located in glass jar body；Rotating disc is located at the lower section of glass jar；Motor one and rotating disc are fixedly mounted；Feed inlet is located at the upper end of glass jar；Discharge port is located at glass pot bottom.Present invention is mainly used for the cultures of photosynthetic bacteria, are capable of providing suitable cultivation temperature, timely adjust the acid-base value of culture solution.
Description
Technical field
The invention belongs to Bacteria Culture technical field, specifically a kind of photosynthetic bacteria cultural method.
Background technology
Photosynthetic bacteria (Photo SyntheticBacteria, PSB) is generally existing in nature, goes out earliest on the earth
The existing prokaryotes with original luminous energy synthetic system are do not put the photosynthetic bacterium of oxygen under anaerobic total
Claim, they using light as the energy, can under anaerobism illumination or aerobic dark condition utilize nature in organic matter, sulfide,
Ammonia etc. carries out photosynthetic microorganism as hydrogen donor and carbon source.Photosynthetic bacteria increases in wastewater treatment, aquaculture, agricultural
Production, medicines and health protection field have been widely used.Photosynthetic bacteria culture generally makees the energy, energy expenditure with artificial light sources long-term irradiation
It is larger.In addition, the light adherence quality that becomes of photosynthetic bacteria can cause photosynthetic bacteria to be attached at culture utensil wall, hinder light through culture
Utensil wall enters in culture apparatus, reduces the intensity of light, influences the utilization ratio of light and the growth rate of photosynthetic bacteria, at present
Photosynthetic bacteria culturing device cannot handle adherent photosynthetic bacteria more thoroughly, in time.
In consideration of it, a kind of photosynthetic bacteria cultural method of the present invention, suitable culture is capable of providing by cultivating instrument
Temperature timely adjusts the acid-base value of culture solution.
Invention content
In order to make up for the deficiencies of the prior art, the present invention proposes a kind of photosynthetic bacteria cultural method, and the present invention mainly uses
It is on the one hand right by cultivating stirring module and the cooperation rotation axis rotation of acid-base accommodation module in instrument in the culture of photosynthetic bacteria
Photosynthetic bacteria is uniformly mixed, on the other hand adherent to optically focused to be effectively treated, while controlling the acid-base value of culture solution, is provided
The suitable for producing environment of photosynthetic bacteria, the simple and practical easy popularization of the photosynthetic bacteria cultural method, the reproduction speed of photosynthetic bacteria are fast.
The technical solution adopted by the present invention to solve the technical problems is：A kind of photosynthetic bacteria culture side of the present invention
Method, the process employs photosynthetic bacteria cultivate instrument, wherein culture instrument include glass jar, luminescent screen, power generation plate, acid-base solution case,
Motor, rotation axis, stirring module, acid-base accommodation module, rotating disc, motor one, inlet port and outlet port, the glass jar are
Drum-shaped, glass jar is for containing culture medium solution and photosynthetic bacteria；The luminescent screen is concentrically mounted on the outside of glass jar；
The power generation plate is concentrically mounted on the outside of luminescent screen；The acid-base solution case is located at the top of cover；The motor
Positioned at the top of soda acid solvent tank；The rotation axis is mounted on motor, and rotation axis passes through acid-base solution case；The stirring
Module is installed on the rotating shaft, and stirring module is for stirring photosynthetic bacteria；The acid-base accommodation module is mounted on rotation axis
Upper end, acid-base accommodation module are located in glass jar body；The rotating disc is located at the lower section of glass jar；The motor one and turn
Moving plate is fixedly mounted；The feed inlet is located at the upper end of glass jar；The discharge port is located at glass pot bottom；
Its specific method uses as follows：
Step 1:Configure photosynthetic bacteria culture solution, allocation ratio：500 grams of culture mediums：80 liters of water：20 liters of strains；
Step 2:After completing step 1, the photosynthetic bacteria culture solution configured is added to by feed inlet in glass jar；
Step 3:After completing step 2, illumination cultivation：Luminescent screen is opened, sufficient light energy, glass jar interlayer are provided
Built-in convex lens carries out optically focused heating, and rotating disc drives glass jar outer layer to be rotated, and to solution of photosynthetic bacteria uniformly add
Temperature；
Step 4:After completing step 3, control cultivation temperature is in 30-38 degree；
Step 5:After completing step 4, motor is opened, stirring rod drives bacterium brush one to rotate, and will be attached to glass top tank structure
Photosynthetic bacteria brush away, avoid occur optically focused it is adherent, influence effective growth of internal photosynthetic bacteria；Detector is according in glass jar
Culture solution acid degree, timely control Miniature retractable cylinder operation, push piston by the pressure containing calcium solution inside stirring rod
Go out, adjusts the acid-base value of culture；
Step 6:After completing step 5, after photosynthetic bacteria is cultivated 3-5 days, photosynthetic bacteria is poured out from discharge port.
Preferably, the glass jar is double glazing tank, and convex lens, convex lens are equipped between the double glazing of glass jar
For the effect that optically focused heats, the glass of glass jar outer layer can rotate, and glass top tank structure is uniformly provided with convex block.It is convex when work
Lens carry out optically focused to luminescent screen and heat, and adjust the temperature in glass jar, promote photosynthetic bacteria optimum growth.
Preferably, the rotation axis is divided into two sections, and epimere is equipped with hollow cavity, hollow cavity and acid-base accommodation module and
Acid-base solution case communicates, and hypomere is solid construction, and hypomere is equipped with helicitic texture.Rotation axis hypomere and stirring module are threadedly coupled,
Stirring module can be moved up and down with rotation axis, the hollow cavity and acid-base accommodation module phase of the setting of epimere rotation axis
It is logical, timely the acid ＆ alkali liquid in acid-base accommodation module can be supplemented.
Preferably, the stirring module includes stirring rod, electrical bar and bacterium brush one, and the stirring rod is mounted on rotation
On axis；The electrical bar is mounted on inside stirring rod；The bacterium brush one is mounted on the front end of stirring rod.When work, stirring
Bar drives bacterium brush one to rotate, and the photosynthetic bacteria for being attached to glass top tank structure is brushed away, and avoids generation optically focused adherent, influences internal photosynthetic
Effective growth of bacterium, in addition electrical bar can control the preference temperature of culture solution.
Preferably, the acid-base solution module includes stirring rod, bacterium brush two, detector, Miniature retractable cylinder and piston,
Hollow cavity is equipped in the stirring rod, hollow cavity is connected with the hollow cavity of rotation axis；The bacterium brush two is installed
In the front end of stirring rod；The detector is mounted in stirring rod；The Miniature retractable cylinder is mounted on the upper of stirring rod
Side；The piston is located in the hollow cavity of stirring rod, and piston is fixedly connected with telescopic cylinder.When work, detector according to
The acid degree of culture solution in glass jar timely controls Miniature retractable cylinder operation, pushes piston by containing inside stirring rod
Calcium solution extrudes, and adjusts the acid-base value of culture solution.
Preferably, the stirring rod lower surface offers circular hole, and rubber clack is equipped in circular hole, and rubber clack compression can be held
It opens.Rubber clack is usually closed, and when only stirring rod internal piston works, rubber clack compression can just open, stirring rod
Internal acid ＆ alkali liquid can just eject, and effectively prevent the nutrient solution inside glass jar and enter in the hollow cavity of stirring rod.
Preferably, the bacterium brush one and bacterium brush two are weak elastomeric bristles.When bristle and convex block collide, elasticity occurs for bristle
Bending is restored again, and the photosynthetic bacteria being attached on bristle is shaken off, and improves the using effect of bacterium brush one and bacterium brush two.
The beneficial effects of the invention are as follows：
1. a kind of photosynthetic bacteria cultural method of the present invention, this method is simple and practical, the cultivation cycle of photosynthetic bacteria
Short, step 5 is cooperated by stirring module and acid-base accommodation module, is on the one hand uniformly mixed to photosynthetic bacteria, favorably
On the other hand adherent to optically focused to be effectively treated in the fast-growth of photosynthetic bacteria, photosynthetic bacteria can obtain sufficient illumination
Condition, while the acid-base value of culture solution is controlled, the suitable for producing environment of photosynthetic bacteria is provided, incubation time is reduced, it is cost-effective,
Improve production capacity.
2. luminescent screen of the present invention is 360 degree of illumination, it ensure that the abundance of illumination condition, be beneficial to photosynthetic bacteria
Growth.
3. the bristle and convex block of bacterium brush one of the present invention and bacterium brush two mutually collide, one He of bacterium brush can will be attached to
Photosynthetic bacteria on bacterium brush two shakes off, and improves bacterium brush one and two working effect of bacterium brush.
4. step 3 of the present invention：It is equipped with convex lens in glass jar, the illumination that luminescent screen is sent out can be gathered
Light heats, and provides suitable temperature, accelerates the flourish of photosynthetic bacteria.
5. power generation plate of the present invention utilizes solar power generation, energy saving, cost is reduced.
Description of the drawings
The invention will be further described below in conjunction with the accompanying drawings.
Fig. 1 is flow chart of the method for the present invention；
Fig. 2 is the sectional view of the present invention；
In figure：Glass jar 1, luminescent screen 2, power generation plate 3, acid-base solution case 4, motor 5, rotation axis 6, stirring module 7, stirring
Bar 71, electrical bar 72, bacterium brush 1, acid-base accommodation module 8, stirring rod 81, bacterium brush 2 82, Miniature retractable cylinder 83, piston 84,
Rotating disc 9, motor 1, feed inlet 11, discharge port 12, convex lens 13, convex block 14.
Specific implementation mode
In order to make the technical means, the creative features, the aims and the efficiencies achieved by the present invention be easy to understand, tie below
Specific implementation mode is closed, the present invention is further explained.
As depicted in figs. 1 and 2, a kind of photosynthetic bacteria cultural method of the present invention, the process employs photosynthetic bacterias
Instrument is cultivated, wherein culture instrument includes glass jar 1, luminescent screen 2, power generation plate 3, acid-base solution case 4, motor 5, rotation axis 6, stirring mould
Block 7, acid-base accommodation module 8, rotating disc 9, motor 1, feed inlet 11 and discharge port 12, the glass jar 1 are drum-shaped,
Glass jar 1 is for containing culture medium solution and photosynthetic bacteria；The luminescent screen 2 is concentrically mounted on the outside of glass jar 1；It is described
Power generation plate 3 be concentrically mounted on the outside of luminescent screen 2；The acid-base solution case 4 is located at the top of cover；The motor 5
Positioned at the top of soda acid solvent tank；The rotation axis 6 is mounted on motor 5, and rotation axis 6 passes through acid-base solution case 4；Described
It stirs module 7 to be mounted in rotation axis 6, stirring module 7 is for stirring photosynthetic bacteria；The acid-base accommodation module 8 is mounted on
The upper end of rotation axis 6, acid-base accommodation module 8 are located in 1 body of glass jar；The rotating disc 9 is located at the lower section of glass jar 1；Institute
The motor 1 and rotating disc 9 stated are fixedly mounted；The feed inlet 11 is located at the upper end of glass jar 1；The discharge port 12
Positioned at 1 bottom of glass jar；
Its specific method uses as follows：
Step 1:Configure photosynthetic bacteria culture solution, allocation ratio：500 grams of culture mediums：80 liters of water：20 liters of strains；
Step 2:After completing step 1, the photosynthetic bacteria culture solution configured is added to glass jar 1 by feed inlet 11
It is interior；
Step 3:After completing step 2, illumination cultivation：Luminescent screen 2 is opened, sufficient light energy is provided, glass jar 1 presss from both sides
Convex lens built in layer carries out optically focused heating, and rotating disc 9 drives 1 outer layer of glass jar to be rotated, and is carried out to solution of photosynthetic bacteria equal
Even heating；
Step 4:After completing step 3, control cultivation temperature is in 30-38 degree；
Step 5:After completing step 4, motor is opened, stirring rod 71 drives bacterium brush 1 to rotate, and will be attached to glass jar 1
The photosynthetic bacteria of inner wall brushes away, and avoids generation optically focused adherent, influences effective growth of internal photosynthetic bacteria；Detector is according to glass
The acid degree of culture solution in tank 1 timely controls Miniature retractable cylinder 83 and works, pushes piston 84 will be inside stirring rod 81
It is extruded containing calcium solution, adjusts the acid-base value of culture；
Step 6:After completing step 5, after photosynthetic bacteria is cultivated 3-5 days, photosynthetic bacteria is poured out from discharge port 12.
As one embodiment of the present invention, the glass jar 1 is double glazing tank 1, the double glazing of glass jar 1
Between be equipped with convex lens 13, convex lens 13 is used for the effect that heats of optically focused, and the glass of 1 outer layer of glass jar can rotate, glass jar 1
Inner wall is uniformly provided with convex block 14.When work, convex lens 13 carries out optically focused to luminescent screen 2 and heats, and adjusts the temperature in glass jar 1,
Promote photosynthetic bacteria optimum growth.
As one embodiment of the present invention, it is two sections that the rotation axis 6, which is divided to, and epimere is equipped with hollow cavity, hollow
Chamber is communicated with acid-base accommodation module 8 and acid-base solution case 4, and hypomere is solid construction, and hypomere is equipped with helicitic texture.Rotation axis 6
Hypomere and stirring module 7 are threadedly coupled, and stirring module 7 can be moved up and down with rotation axis 6, and epimere rotation axis 6 is arranged
Hollow cavity communicated with acid-base accommodation module 8, timely the acid ＆ alkali liquid in acid-base accommodation module 8 can be supplemented.
As one embodiment of the present invention, the stirring module 7 includes stirring rod 71, electrical bar 72 and bacterium brush one
73, the stirring rod 71 is mounted in rotation axis 6；The electrical bar 72 is mounted on inside stirring rod 71；The bacterium brush
One 73 are mounted on the front end of stirring rod 71.When work, stirring rod 71 drives bacterium brush 1 to rotate, and will be attached to 1 inner wall of glass jar
Photosynthetic bacteria brushes away, and avoids generation optically focused adherent, influences effective growth of internal photosynthetic bacteria, electrical bar 72 can control in addition
The preference temperature of culture solution.
As one embodiment of the present invention, the acid-base solution module includes stirring rod 81, bacterium brush 2 82, detection
Device, Miniature retractable cylinder 83 and piston 84, the stirring rod 81 is interior to be equipped with hollow cavity, the sky of hollow cavity and rotation axis 6
Chambers of the heart room is connected；The bacterium brush 2 82 is mounted on the front end of stirring rod 81；The detector is mounted in stirring rod 81；
The Miniature retractable cylinder 83 is mounted on the top of stirring rod 81；The piston 84 is located at the hollow cavity of stirring rod 81
Interior, piston 84 is fixedly connected with Miniature retractable cylinder 83.When work, detector according to the acid degree of the culture solution in glass jar 1,
Timely control Miniature retractable cylinder 83 works, and pushes piston 84 that will be extruded containing calcium solution inside stirring rod 81, adjusts culture
Acid-base value.
As one embodiment of the present invention, 81 lower surface of stirring rod offers circular hole, is equipped in circular hole
Rubber clack, rubber clack compression can open.Rubber clack is usually closed, when only 81 internal piston 84 of stirring rod works,
Rubber clack compression can just open, and the acid ＆ alkali liquid inside stirring rod 81 can just eject, and effectively prevent the nutrition inside glass jar 1
Liquid enters in the hollow cavity of stirring rod 81.
As one embodiment of the present invention, the bacterium brush 1 and bacterium brush 2 82 are weak elastomeric bristles.Bristle and
When convex block 14 collides, bristle is resiliently deflected to restore again, and the photosynthetic bacteria being attached on bristle is shaken off, and improves bacterium brush 1
With the using effect of bacterium brush 2 82.
In use, photosynthetic bacteria and culture solution is added from feed inlet 11, start motor 5, luminescent screen 2 is powered, and motor 5 rotates
Stirring module 7 is driven to rotate, stirring rod 71 drives bacterium brush 1 to rotate, and the photosynthetic bacteria for being attached to 1 inner wall of glass jar is brushed away, is kept away
It is adherent to exempt from generation optically focused, influences effective growth of internal photosynthetic bacteria；Simultaneously acid-base accommodation module 8 also rotate, detector according to
The acid degree of culture solution in glass jar 1 timely controls Miniature retractable cylinder 83 and works, pushes piston 84 will be in stirring rod 81
Portion is extruded containing calcium solution, adjusts the acid-base value of culture, the convex lens 13 being equipped in 1 interlayer of glass jar adds culture solution
Temperature, rotating disc 9 is rotated, with the glass jar 1 of outer layer, is evenly heated, and after culture, is flowed out from discharge port 12.
The basic principles, main features and advantages of the invention have been shown and described above.The technical staff of the industry should
Understand, the present invention is not limited to the above embodiments, and the above embodiments and description only describe the originals of the present invention
Reason, without departing from the spirit and scope of the present invention, various changes and improvements may be made to the invention, these changes and improvements
It all fall within the protetion scope of the claimed invention.The claimed scope of the invention is by appended claims and its equivalent circle
It is fixed.
Claims (7)
1. a kind of photosynthetic bacteria cultural method, it is characterised in that：The process employs photosynthetic bacterias to cultivate instrument, wherein culture instrument packet
Include glass jar (1), luminescent screen (2), power generation plate (3), acid-base solution case (4), motor (5), rotation axis (6), stirring module (7),
Acid-base accommodation module (8), rotating disc (9), motor one (10), feed inlet (11) and discharge port (12), the glass jar (1) are
Drum-shaped, glass jar (1) is for containing culture medium solution and photosynthetic bacteria；The luminescent screen (2) is concentrically mounted on glass jar
(1) outside；The power generation plate (3) is concentrically mounted on the outside of luminescent screen (2)；The acid-base solution case (4) is located at tank
The top of lid；The motor (5) is located at the top of soda acid solvent tank；The rotation axis (6) is mounted on motor (5), is turned
Moving axis (6) passes through acid-base solution case (4)；The stirring module (7) is mounted in rotation axis (6), and stirring module (7) is for stirring
Mix photosynthetic bacteria；The acid-base accommodation module (8) is mounted on the upper end of rotation axis (6), and acid-base accommodation module (8) is located at glass
Tank (1) is internal；The rotating disc (9) is located at the lower section of glass jar (1)；The motor one (10) and rotating disc (9) are fixed
Installation；The feed inlet (11) is located at the upper end of glass jar (1)；The discharge port (12) is located at glass jar (1) bottom；
Its specific method uses as follows：
Step 1:Configure photosynthetic bacteria culture solution, allocation ratio：500 grams of culture mediums：80 liters of water：20 liters of strains；
Step 2:After completing step 1, the photosynthetic bacteria culture solution configured is added to glass jar (1) by feed inlet (11)
It is interior；
Step 3:After completing step 2, illumination cultivation：Luminescent screen (2) is opened, sufficient light energy, glass jar (1) folder are provided
Convex lens built in layer carries out optically focused heating, and rotating disc (9) drives glass jar (1) outer layer to be rotated；
Step 4:After completing step 3, control cultivation temperature is in 30-38 degree；
Step 5:After completing step 4, motor is opened, stirring module (7) and acid-base accommodation module (8) train photosynthetic bacteria
Nutrient solution is stirred and adjusts acid-base value, while handling photosynthetic bacteria is adherent；
Step 6:After completing step 5, after photosynthetic bacteria is cultivated 3-5 days, photosynthetic bacteria is poured out from discharge port (12).
2. a kind of photosynthetic bacteria cultural method according to claim 1, it is characterised in that：The glass jar (1) is double
Layer glass jar (1) is equipped with convex lens (13) between the double glazing of glass jar (1), and convex lens (13) is heated for optically focused, glass
The glass of tank (1) outer layer can rotate, and glass jar (1) inner wall is uniformly provided with convex block (14).
3. a kind of photosynthetic bacteria cultural method according to claim 1, it is characterised in that：The rotation axis (6) is divided into
Two sections, epimere is equipped with hollow cavity, and hollow cavity is communicated with acid-base accommodation module (8) and acid-base solution case (4), and hypomere is solid
Structure, hypomere are equipped with helicitic texture.
4. a kind of photosynthetic bacteria cultural method according to claim 2, it is characterised in that：Stirring module (7) packet
Stirring rod (71), electrical bar (72) and bacterium brush one (73) are included, the stirring rod (71) is mounted in rotation axis (6)；Described
It is internal that electrical bar (72) is mounted on stirring rod (71)；The bacterium brush one (73) is mounted on the front end of stirring rod (71).
5. a kind of photosynthetic bacteria cultural method according to claim 3, it is characterised in that：The acid-base accommodation module
(8) include stirring rod (81), bacterium brush two (82), detector, Miniature retractable cylinder (83) and piston (84), the stirring rod
(81) hollow cavity is equipped in, hollow cavity is connected with the hollow cavity of rotation axis (6)；The bacterium brush two (82) is mounted on
The front end of stirring rod (81)；The Miniature retractable cylinder (83) is mounted on the top of stirring rod (81)；The piston (84)
In the hollow cavity of stirring rod (81), piston (84) is fixedly connected with Miniature retractable cylinder (83).
6. a kind of photosynthetic bacteria cultural method according to claim 5, it is characterised in that：Described stirring rod (81) following table
Face offers circular hole, and rubber clack is equipped in circular hole, and rubber clack compression can open.
7. a kind of photosynthetic bacteria cultural method according to claim 2,4 or 5, it is characterised in that：The bacterium brush one
(73) and bacterium brush two (82) is weak elastomeric bristles.
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| CN201810202674.8A CN108485941B (en) | 2018-03-13 | 2018-03-13 | Photosynthetic bacterium culture method |
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| CN201810202674.8A CN108485941B (en) | 2018-03-13 | 2018-03-13 | Photosynthetic bacterium culture method |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109762723A (en) * | 2019-03-23 | 2019-05-17 | 武汉轻工大学 | A kind of device for photosynthetic bacteria culture |
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| CN201915083U (en) * | 2010-09-13 | 2011-08-03 | 江苏邦德生物科技发展有限公司 | Efficient culturing device for photosynthetic bacteria |
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| CN108485941B (en) | 2021-09-21 |
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