CN109762723A - A kind of device for photosynthetic bacteria culture - Google Patents
A kind of device for photosynthetic bacteria culture Download PDFInfo
- Publication number
- CN109762723A CN109762723A CN201910224395.6A CN201910224395A CN109762723A CN 109762723 A CN109762723 A CN 109762723A CN 201910224395 A CN201910224395 A CN 201910224395A CN 109762723 A CN109762723 A CN 109762723A
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- China
- Prior art keywords
- main body
- photosynthetic bacteria
- described device
- device main
- culture medium
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- 241000894006 Bacteria Species 0.000 title claims abstract description 59
- 230000000243 photosynthetic effect Effects 0.000 title claims abstract description 36
- 238000001914 filtration Methods 0.000 claims abstract description 18
- 239000012535 impurity Substances 0.000 claims abstract description 18
- 238000000746 purification Methods 0.000 claims abstract description 17
- 239000007788 liquid Substances 0.000 claims abstract description 16
- 239000007921 spray Substances 0.000 claims abstract description 8
- 238000003756 stirring Methods 0.000 claims description 12
- 239000012530 fluid Substances 0.000 claims description 9
- 239000003381 stabilizer Substances 0.000 claims description 5
- 230000005611 electricity Effects 0.000 claims 1
- 239000001963 growth medium Substances 0.000 abstract description 30
- 244000005700 microbiome Species 0.000 abstract description 5
- 238000012258 culturing Methods 0.000 abstract description 3
- 230000008676 import Effects 0.000 abstract 1
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 4
- 238000000034 method Methods 0.000 description 4
- 230000001954 sterilising effect Effects 0.000 description 4
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 229910052757 nitrogen Inorganic materials 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 description 1
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 description 1
- 239000005864 Sulphur Substances 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 239000012190 activator Substances 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000000975 bioactive effect Effects 0.000 description 1
- 244000144987 brood Species 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 239000000356 contaminant Substances 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 229910052739 hydrogen Inorganic materials 0.000 description 1
- 239000001257 hydrogen Substances 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 238000010672 photosynthesis Methods 0.000 description 1
- 230000029553 photosynthesis Effects 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 239000006041 probiotic Substances 0.000 description 1
- 235000018291 probiotics Nutrition 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
- 238000000844 transformation Methods 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a kind of devices for photosynthetic bacteria culture, including apparatus main body, the top of described device main body, which is run through, feeding inlet, the top side of the feeding inlet is connected with pan feeding lid by movable axis, and handle is fixed at the top of the pan feeding lid, observation window is provided with above the outer surface of described device main body, station is provided with below the outer surface of described device main body, described device main body is internally provided with purification room, user can will purify indoor culture medium progress miscellaneous bacteria by station unlatching ultraviolet sterilizer and kill, the purity of the culture medium handled needed for guaranteeing, culturing room is entered by impurity filtering net by the culture medium killed, guarantee the purity of culture medium；The photosynthetic bacteria of required culture imports liquid reserve tank by filtering, from bacterium solution mouth, and is uniformly sprayed by spray head in the culture medium in culturing room；It is stirred at a constant temperature, grows pure photosynthetic bacteria microorganism by the discharge port output of bottom of device.
Description
Technical field
The present invention relates to microbial reproduction technical field, specially a kind of device for photosynthetic bacteria culture.
Background technique
Photosynthetic bacteria is a kind of probiotics, is played an important role in carbon, nitrogen, the sulphur transformations circulation of nature, it
Rich in protein, vitamin and various bioactive components and physiological activator, has and carry out photosynthesis, fermentation and consolidate
Nitrogen produces the functions such as hydrogen.Therefore, photosynthetic bacteria environmental protection, agricultural, medicine, aquatic products and in terms of have higher answer
With value.Photosynthetic bacteria is mainly manually cultivated at present, and the factor for influencing photosynthetic bacteria culture is more, such as bacterial strain type, culture
Base, condition of culture etc. usually require culture medium in existing photosynthetic bacteria incubation, due to supporting in culture medium containing different
Divide substance, these substances are inevitably mixed into other miscellaneous bacterias since purity is not high, storage practice is different, will affect so photosynthetic thin
The cultivation quality of bacteria microorganism entirety needs to sterilize culture medium in advance, removes miscellaneous bacteria.Existing sterilizing methods are generally adopted
With the sterilizing means of high temperature and pressure, it is be easy to cause danger, needs to improve sterilizing methods；In addition, existing photosynthetic bacteria
When microorganism is cultivated, most of is all simply to be stirred photosynthetic bacteria strain with culture medium, photosynthetic in this way
Bacterium absorbs the inefficient of culture medium, the overlong time cultivated needed for causing.
Summary of the invention
The purpose of the present invention is to provide a kind of devices for photosynthetic bacteria culture, to solve to mention in above-mentioned background technique
Culture medium purity is not high out, internal containing other miscellaneous bacterias, influences the cultivation quality of whole microorganism；Culture medium and photosynthetic thin
Bacterium is simply stirred, and the time that will lead to inefficient, the required cultivation that photosynthetic bacteria absorbs culture medium is extended
Problem.
To achieve the above object, the invention provides the following technical scheme: it is a kind of for photosynthetic bacteria culture device, including
Apparatus main body, through there is feeding inlet, the top side of the feeding inlet is connected with by movable axis at the top of described device main body
Pan feeding lid, and handle is fixed at the top of the pan feeding lid, it is provided with observation window above the outer surface of described device main body,
Station is provided with below the outer surface of described device main body；The side of described device main body is provided with liquid reserve tank, and the storage
The side of liquid case is through there is bacterium solution mouth, and the bottom of described device main body, which is connected with, cultivates storehouse, below the outer surface for cultivating storehouse
Through there is discharge port, the bottom for cultivating storehouse is connected with stabilizer blade；Cabinet, the dress are fixed among the bottom for cultivating storehouse
That sets main body is internally provided with purification room, and the interior side of the purification room is provided with ultraviolet sterilizer, the purification room
Bottom is connected with connecting tube；The liquid reserve tank is internally provided with fluid reservoir, and the bottom of the fluid reservoir is through there is spray head, institute
That states cultivation storehouse is internally provided with raising chamber, and the raising chamber is internally provided with thermostat, and the cabinet is internally provided with
Motor, and the output end of the motor is connected with stirring rod.
Preferably, the bacterium solution mouth is internally provided with filter screen, and in the cross-sectional area of the filter screen and bacterium solution mouth
The cross-sectional area in portion is equal.
Preferably, the lower inside of the purification room is provided with impurity filtering net.
Preferably, the stirring rod is horizontally set at the lower inside of raising chamber, and the bottom of the stirring rod is through training
The bottom of brood chamber and the output end of motor connect.
Preferably, the cross-sectional area of the impurity filtering net and the cross-sectional area of connecting tube are equal, and the contaminant filter
Net is connected in the form being socketed with connecting tube.
Preferably, there are two the thermostat settings, and two thermostats are in same level height.
Compared with prior art, the beneficial effects of the present invention are: device of this kind for photosynthetic bacteria culture is provided with purple
Culture medium is imported into the inside of purification room by feeding inlet, opens ultraviolet light by outside line sterilizer and impurity filtering net, user
Sterilizer quickly kills the miscellaneous bacteria inside culture medium, and the culture medium after killing miscellaneous bacteria passes through impurity filtering net, filters
Suspended matter and other impurities in culture medium, guarantee the purity of culture medium in this way, so that it is not high to solve culture medium purity
Problem；It is imported into culturing room by the culture medium that ultraviolet light is killed by connecting tube, waits photosynthetic bacteria strain to be added；Required training
Feeding photosynthetic bacteria strain is imported into liquid reserve tank by the filter screen of bacterium solution mouth；The bottom of fluid reservoir is run through and is had in the present apparatus
Strain, can be converted to smaller form by spray head, it is evenly dispersed in the medium, convenient for the growth of micropopulation, solve
Directly culture medium and photosynthetic bacteria are simply stirred, and cause photosynthetic bacteria absorb culture medium it is inefficient,
The problem of time of required cultivation extends.
Detailed description of the invention
Fig. 1 is schematic structural view of the invention；
Fig. 2 is apparatus of the present invention body interior structural schematic diagram；
Fig. 3 is the partial enlargement structural representation of A of the present invention.
In figure: 1, apparatus main body；2, feeding inlet；3, pan feeding lid；4, handle；5, movable axis；6, observation window；7, it operates
Platform；8, liquid reserve tank；9, bacterium solution mouth；10, filter screen；11, storehouse is cultivated；12, discharge port；13, cabinet；14, stabilizer blade；15, ultraviolet light
Sterilizer；16, room is purified；17, impurity filtering net；18, fluid reservoir；19, spray head；20, connecting tube；21, thermostat；22, motor；
23, stirring rod；24, raising chamber.
Specific embodiment
Following will be combined with the drawings in the embodiments of the present invention, and technical solution in the embodiment of the present invention carries out clear, complete
Site preparation description, it is clear that described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.It is based on
Embodiment in the present invention, it is obtained by those of ordinary skill in the art without making creative efforts every other
Embodiment shall fall within the protection scope of the present invention.
In the present invention: thermostat model ATS-MH-2800A/D/E, ultraviolet sterilizer model SYZW150-24.
Fig. 1-3 is please referred to, the present invention provides a kind of technical solution: a kind of device for photosynthetic bacteria culture, including dress
Set main body 1, feeding inlet 2, pan feeding lid 3, handle 4, movable axis 5, observation window 6, station 7, liquid reserve tank 8, bacterium solution mouth 9, filtering
Net 10 cultivates storehouse 11, discharge port 12, cabinet 13, stabilizer blade 14, ultraviolet sterilizer 15, purification room 16, impurity filtering net 17, storage
Liquid chamber 18, spray head 19, connecting tube 20, thermostat 21, motor 22, stirring rod 23 and raising chamber 24, the top of described device main body 1
Through there is feeding inlet 2, the top side of the feeding inlet 2 is connected with pan feeding lid 3, and the pan feeding lid 3 by movable axis 5
Top be fixed with handle 4, be provided with observation window 6, the appearance of described device main body 1 above the outer surface of described device main body 1
It is provided with station 7 below face, the side of described device main body 1 is provided with liquid reserve tank 8, and the side of the liquid reserve tank 8 is run through
There is bacterium solution mouth 9, the bacterium solution mouth 9 is internally provided with filter screen 10, and in the cross-sectional area of the filter screen 10 and bacterium solution mouth 9
The cross-sectional area in portion is equal, can more comprehensively be filtered to culture microorganism fungus kind when being worked；It is described
The bottom of apparatus main body 1, which is connected with, cultivates storehouse 11, and running through below the outer surface for cultivating storehouse 11 has discharge port 12, the cultivation
The bottom in storehouse 11 is connected with stabilizer blade 14, is fixed with cabinet 13, the inside of described device main body 1 among the bottom for cultivating storehouse 11
It is provided with purification room 16, the lower inside of the purification room 16 is provided with impurity filtering net 17, can be with when being worked
Culture solution inside purification room 16 is filtered, the cross-sectional area of the impurity filtering net 17 and the cross-sectional area of connecting tube 20
Equal, the top of the connecting tube 20 is provided with control valve, and the impurity filtering net 17 is in the form of socket and connecting tube
The connection of 20 upper ends, can increase the efficiency of work when being worked, and the interior side of the purification room 16 is provided with purple
The bottom of outside line sterilizer 15, the purification room 16 is connected with connecting tube 20, and the top of the connecting tube 20 is provided with control valve
Door；The liquid reserve tank 8 is internally provided with fluid reservoir 18, and the bottom of the fluid reservoir 18 is through having spray head 19, the cultivation
Storehouse 11 is internally provided with raising chamber 24, and the raising chamber 24 is internally provided with thermostat 21, and the thermostat 21 is provided with
Two, and two thermostats 21 are in same level height, it can be preferably to raising chamber 24 when being worked
Internal photosynthetic bacteria provides required temperature, and the cabinet 13 is internally provided with motor 22, and the output end of the motor 22
It is connected with stirring rod 23, the stirring rod 23 is horizontally set at the lower inside of raising chamber 24, and the bottom of the stirring rod 23
Bottom through raising chamber 24 is connect with the output end of motor 22, can increase the efficiency of work when being worked.
The impurity filtering net 17 is connected in the form of socket with 20 upper end of connecting tube, when 17 surface of impurity filtering net retains
Sundries it is excessive when, open material mouth lid 3, taking-up be rinsed with water it is clean after be put in device, be then turned on ultraviolet sterilizer 15
Sterilization treatment is carried out to its surface, circulation is carried out and reuses.
Working principle: firstly, should check whether all parts performance is normal before use device, each component capabilities to be checked
After normal, device is placed in assigned work place, after placing device, is powered on, user is opened by handle 4
Pan feeding lid 3 closes the valve in connecting tube 20, when required culture solution is imported the inside of purification room 16 by feeding inlet 2,
User can open ultraviolet sterilizer 15 by station 7 and quickly be killed the miscellaneous bacteria in culture medium, and by miscellaneous
Matter filter screen 17 opens control valve by connecting tube 20() it enters in raising chamber 24, wait the inoculation of photosynthetic bacteria strain；On
It states operation to be filtered required culture medium by impurity filtering net 17, purification and impurity removal has been carried out to culture medium again, protect
The purity of culture medium is demonstrate,proved；After above-mentioned culture medium knocks out miscellaneous bacteria, ultraviolet sterilizer 15 is closed, opens the control on 20 top of connecting tube
Valve processed will be killed the culture medium after miscellaneous bacteria and be imported in raising chamber 24 by connecting tube 20, while by the photosynthetic bacteria of required dispensing
Strain is imported in the fluid reservoir 18 inside liquid reserve tank 8 by bacterium solution mouth 9, then will be photosynthetic thin by the perforative spray head 19 in 18 bottom of fluid reservoir
Bacterium strain is sprayed in culture medium, then, starts motor 22 by station 7, motor 22 drives stirring rod 23 to raising chamber 24
Internal photosynthetic bacteria is stirred, and promotes the absorption to culture medium.
It although an embodiment of the present invention has been shown and described, for the ordinary skill in the art, can be with
A variety of variations, modification, replacement can be carried out to these embodiments without departing from the principles and spirit of the present invention by understanding
And modification, the scope of the present invention is defined by the appended.
Claims (6)
1. a kind of device for photosynthetic bacteria culture, including apparatus main body (1), it is characterised in that: described device main body (1)
Through having feeding inlet (2), the top side of the feeding inlet (2) is connected with pan feeding lid (3) by movable axis (5) at top, and
It is fixed with handle (4) at the top of the pan feeding lid (3), is provided with observation window above the outer surface of described device main body (1)
(6), it is provided with station (7) below the outer surface of described device main body (1), the side of described device main body (1) is provided with storage
Liquid case (8), and the side of the liquid reserve tank (8), through having bacterium solution mouth (9), the bottom of described device main body (1) is connected with cultivation
Storehouse (11), through having discharge port (12), the bottom for cultivating storehouse (11) is connected with for the outer surface lower section for cultivating storehouse (11)
Stabilizer blade (14), it is described cultivate storehouse (11) bottom among be fixed with cabinet (13), being internally provided with for described device main body (1) mentions
The interior side of pure room (16), purification room (16) is provided with ultraviolet sterilizer (15), the bottom of purification room (16)
It is connected with connecting tube (20), connecting tube (20) top is provided with control valve；The liquid reserve tank (8) is internally provided with storage
Liquid chamber (18), and the bottom of the fluid reservoir (18), through having spray head (19), cultivation storehouse (11) is internally provided with cultivation
Room (24), the raising chamber (24) are internally provided with thermostat (21), and the cabinet (13) is internally provided with motor (22),
And the output end of the motor (22) is connected with stirring rod (23).
2. a kind of device for photosynthetic bacteria culture according to claim 1, it is characterised in that: the bacterium solution mouth (9)
Be internally provided with filter screen (10), and the cross-sectional area phase that the cross-sectional area of the filter screen (10) and bacterium solution mouth (9) are internal
Deng.
3. a kind of device for photosynthetic bacteria culture according to claim 1, it is characterised in that: the purification room (16)
Lower inside be provided with impurity filtering net (17).
4. a kind of device for photosynthetic bacteria culture according to claim 1, it is characterised in that: the stirring rod (23)
It is horizontally set at the lower inside of raising chamber (24), and bottom and electricity of the bottom of the stirring rod (23) through raising chamber (24)
The output end of machine (22) connects.
5. a kind of device for photosynthetic bacteria culture according to claim 3, it is characterised in that: the impurity filtering net
(17) cross-sectional area is equal with the cross-sectional area of connecting tube (20), and the impurity filtering net (17) is in the form of socket and company
Take over (20) connection.
6. a kind of device for photosynthetic bacteria culture according to claim 1, it is characterised in that: the thermostat (21)
There are two settings, and two thermostats (21) are in same level height.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910224395.6A CN109762723B (en) | 2019-03-23 | 2019-03-23 | Device for photosynthetic bacteria culture |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910224395.6A CN109762723B (en) | 2019-03-23 | 2019-03-23 | Device for photosynthetic bacteria culture |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN109762723A true CN109762723A (en) | 2019-05-17 |
| CN109762723B CN109762723B (en) | 2024-04-05 |
Family
ID=66458741
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910224395.6A Active CN109762723B (en) | 2019-03-23 | 2019-03-23 | Device for photosynthetic bacteria culture |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109762723B (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112662531A (en) * | 2021-01-05 | 2021-04-16 | 景德镇学院 | Filter equipment is used in microorganism collection |
| CN113832011A (en) * | 2021-09-22 | 2021-12-24 | 川北医学院 | Reduce bacterial solution inoculation device that other bacterial sneaked into |
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| CN101538587A (en) * | 2009-03-23 | 2009-09-23 | 河南农业大学 | Method for continuously producing hydrogen with photosynthetic bacteria and apparatus thereof |
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| CN209957772U (en) * | 2019-03-23 | 2020-01-17 | 武汉轻工大学 | A device for photosynthetic bacteria culture |
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2019
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| CN101538587A (en) * | 2009-03-23 | 2009-09-23 | 河南农业大学 | Method for continuously producing hydrogen with photosynthetic bacteria and apparatus thereof |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112662531A (en) * | 2021-01-05 | 2021-04-16 | 景德镇学院 | Filter equipment is used in microorganism collection |
| CN113832011A (en) * | 2021-09-22 | 2021-12-24 | 川北医学院 | Reduce bacterial solution inoculation device that other bacterial sneaked into |
| CN113832011B (en) * | 2021-09-22 | 2024-06-25 | 川北医学院 | Bacteria solution inoculation device for reducing mixing of other strains |
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| Publication number | Publication date |
|---|---|
| CN109762723B (en) | 2024-04-05 |
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