CN103255073A - Method for rapid propagation of high purity photosynthetic bacterium - Google Patents
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- CN103255073A CN103255073A CN2012100343869A CN201210034386A CN103255073A CN 103255073 A CN103255073 A CN 103255073A CN 2012100343869 A CN2012100343869 A CN 2012100343869A CN 201210034386 A CN201210034386 A CN 201210034386A CN 103255073 A CN103255073 A CN 103255073A
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- 241000894006 Bacteria Species 0.000 title claims abstract description 85
- 230000000243 photosynthetic effect Effects 0.000 title claims abstract description 82
- 238000000034 method Methods 0.000 title claims abstract description 66
- 239000007788 liquid Substances 0.000 claims abstract description 53
- 238000004659 sterilization and disinfection Methods 0.000 claims abstract description 30
- 238000005286 illumination Methods 0.000 claims abstract description 21
- 230000001954 sterilising effect Effects 0.000 claims abstract description 19
- 238000002360 preparation method Methods 0.000 claims abstract description 18
- 238000011081 inoculation Methods 0.000 claims abstract description 15
- OSVXSBDYLRYLIG-UHFFFAOYSA-N dioxidochlorine(.) Chemical compound O=Cl=O OSVXSBDYLRYLIG-UHFFFAOYSA-N 0.000 claims abstract description 8
- 238000005457 optimization Methods 0.000 claims abstract description 5
- 239000004155 Chlorine dioxide Substances 0.000 claims abstract description 4
- 235000019398 chlorine dioxide Nutrition 0.000 claims abstract description 4
- 235000015097 nutrients Nutrition 0.000 claims description 53
- 238000009395 breeding Methods 0.000 claims description 31
- 230000001488 breeding effect Effects 0.000 claims description 31
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 18
- 230000001580 bacterial effect Effects 0.000 claims description 18
- 239000002054 inoculum Substances 0.000 claims description 14
- 150000002978 peroxides Chemical class 0.000 claims description 13
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 13
- 239000002994 raw material Substances 0.000 claims description 6
- 239000003206 sterilizing agent Substances 0.000 claims description 6
- 239000001888 Peptone Substances 0.000 claims description 3
- 108010080698 Peptones Proteins 0.000 claims description 3
- DPDMMXDBJGCCQC-UHFFFAOYSA-N [Na].[Cl] Chemical compound [Na].[Cl] DPDMMXDBJGCCQC-UHFFFAOYSA-N 0.000 claims description 3
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 claims description 3
- 229910052921 ammonium sulfate Inorganic materials 0.000 claims description 3
- 235000011130 ammonium sulphate Nutrition 0.000 claims description 3
- 229940041514 candida albicans extract Drugs 0.000 claims description 3
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 claims description 3
- 239000011790 ferrous sulphate Substances 0.000 claims description 3
- 235000003891 ferrous sulphate Nutrition 0.000 claims description 3
- BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 claims description 3
- 229910000359 iron(II) sulfate Inorganic materials 0.000 claims description 3
- 235000019319 peptone Nutrition 0.000 claims description 3
- 230000029058 respiratory gaseous exchange Effects 0.000 claims description 3
- 238000003756 stirring Methods 0.000 claims description 3
- 239000012138 yeast extract Substances 0.000 claims description 3
- 230000000813 microbial effect Effects 0.000 abstract description 6
- 208000015181 infectious disease Diseases 0.000 abstract description 2
- 230000001105 regulatory effect Effects 0.000 abstract description 2
- 239000000645 desinfectant Substances 0.000 abstract 1
- 238000005516 engineering process Methods 0.000 description 8
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- 238000011109 contamination Methods 0.000 description 3
- 238000012258 culturing Methods 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 238000006213 oxygenation reaction Methods 0.000 description 3
- 241001465754 Metazoa Species 0.000 description 2
- 238000011534 incubation Methods 0.000 description 2
- 244000144977 poultry Species 0.000 description 2
- 238000001556 precipitation Methods 0.000 description 2
- 150000003839 salts Chemical class 0.000 description 2
- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 description 1
- 241000251468 Actinopterygii Species 0.000 description 1
- 241000143060 Americamysis bahia Species 0.000 description 1
- 241000238557 Decapoda Species 0.000 description 1
- 208000027418 Wounds and injury Diseases 0.000 description 1
- 238000009360 aquaculture Methods 0.000 description 1
- 244000144974 aquaculture Species 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
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- 230000002550 fecal effect Effects 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 238000009630 liquid culture Methods 0.000 description 1
- 244000144972 livestock Species 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
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- 244000005700 microbiome Species 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 230000000452 restraining effect Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 235000015170 shellfish Nutrition 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 235000000053 special nutrition Nutrition 0.000 description 1
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Abstract
The invention relates to a microbial preparation field, in particular to a method for rapid propagation of a high purity photosynthetic bacterium. Specifically, the method includes that: a liquid medium is prepared by optimization, and employs chlorine dioxide as a disinfectant, so that the purpose of comprehensive sterilization of the liquid medium can be realized without high temperature and high pressure sterilization; a high purity photosynthetic bacterium is selected as a provenance, and inoculation is carried out in an aseptic environment, thus effectively avoiding mixed microbial infection; during culture, the photosynthetic bacterium is placed on a rotary shaker to undergo shaking culture, the temperature and illumination of a photosynthetic bacterium culture environment are effectively regulated to achieve the purpose of rapid propagation of a finished product. The photosynthetic bacteria fostered by the method provided in the invention all maintain superior quality and high purity.
Description
Technical field
The present invention relates to a kind of microbial preparation field, be specifically related to a kind of high purity photosynthetic bacterium method of breeding fast.
Technical background
Photosynthetic bacterium, be the prokaryotic organism with original luminous energy synthetic system the most ancient on the earth, photosynthetic bacterium is distributed widely in marsh, pond, lake, river, ditch, ocean and the soil, the ability of restraining oneself high temperature, high salt and low temperature, less salt is strong especially, even in the hot spring of 90 ℃ of high temperature, in the salt lake that contains 30% salinity, reaching deeply in 2000 meters the deep-sea, on the ice-bound seashore in the South Pole, can both find the trace of photosynthetic bacterium.
The history of existing 30 or 40 years of the action oriented research of photosynthetic bacterium and productivity research.Japanology must be early, the sixties in 20th century, the variation of bacterium number when Japanese scientist has observed the fecal sewage of high density place naturally, the ecological succession that has at first disclosed natural organic sewage and be by microorganism is cleaned.Through further research, the effect that the thalline of having found photosynthetic bacterium again has tangible promotion growth and improves surviving rate the young of poultry, domestic animal and fish, shrimp, crab, shellfish, thus opened up new field for the comprehensive utilization of photosynthetic bacterial thallus.
China starts late to the research of photosynthetic bacterium, only limits to the separation of bacterial classification at first and be used for high-concentration sewage handle, and many professionals had carried out otherwise applied research again afterwards, had obtained a large amount of achievements.People such as the Zhang Daonan of Shanghai Aquatic Products Univ. 9CN) in 1987 apply it on shrimps grow seedlings, and test group has improved 19.3% than the control group surviving rate as a result, and distortion ratio has improved 18.5%.Now, photosynthetic bacterium as a kind of generally attention that has special nutrition, promotes growth, disease-resistant factor and high-level efficiency purifying cultivation sewage and environment and the nontoxic special bacterium of aquatic animal have been subjected to people with like.Therefore, it is widely used in the relevant field of aquaculture, livestock and poultry cultivation and water treatment.
At present, photosynthetic bacterium product on the market is various, and in these photosynthetic bacterium products, production technology process is variation especially, and application number is 02135435.9 " preparation method of photosynthetic bacteria preparation ", invents in the described technology according to this, in 80～100 hours between the photosynthetic bacterium incubation period, the 25 minutes needs in every interval stirred 6 minutes, if these work by manually finishing, are to be difficult to accomplish.
Application number is 03135857.8 " photosynthetic bacterium high efficiency preparation method ", substratum is to pass through autoclave sterilization in this working of an invention process, the culturing process of photosynthetic bacterium also needs the fermenting jar to finish, and a large amount of power consumptions had just significantly increased production cost during the specific equipment that involves great expense dropped into and produces.
Application number is 200410048678.3 " preparation method of high density photosynthetic bacteria preparation ", and this is invented, and inoculation is open in the described technology, and this has just increased the probability of microbial contamination; Inoculum size is up to 10～20%, and the bacterial classification input cost is too high; In addition, in these in culturing process 72～120 hours of invention, shake up once every 5～8 hours needs, such work allow manually realize also unrealistic.
Application number is 200710011997.0 " a kind of photosynthetic bacterium enriched substratum ", and this invention substratum in implementation process need pass through autoclave sterilization, power consumption in a large number during the specific equipment that also just need involve great expense drops into and produces.
Application number is 200810137445.9 " cultural method of a kind of photosynthetic bacterium ", invent in the described technology according to this, the culturing process of photosynthetic bacterium needs oxygenation, if yet the airiness pipeline does not have filtering function in the process of oxygenation, also will carry a lot of assorted bacterium toward nutrient solution during oxygenation, then strengthen the infection rate of nutrient solution and influence the quality of product, also influence the result of use of product relatively.
Application number is 201010618451.3 " the simple and easy cultural method of a kind of photosynthetic bacterium ", and this invention needs special-purpose distilled water, and culture cycle also needs 7 days more than.
Application number is 201010590805.8 " the photosynthetic bacterium fermentation technique under a kind of natural ecological condition ", invent in the described technology according to this, cultivating water sterilizes through sterilizing agent, but when inoculation, under gnotobasis, do not carry out, equally also will infect other assorted bacterium, influence the quality of product then.
Summary of the invention
The method that the objective of the invention is to overcome the deficiencies in the prior art and provide brand-new a kind of high purity photosynthetic bacterium to breed fast.Specifically comprise: the optimization of liquid nutrient medium preparation, the sterilization method of liquid nutrient medium, the selecting for use of culture vessel, the selection of bacterial classification, inoculation and inoculum size under the gnotobasis are cultivated the temperature of environment, illumination regulation and control and culture cycle during cultivation by rotary shaking table.
Characteristics of the present invention are that liquid nutrient medium is through optimizing preparation, liquid nutrient medium is to make sterilizing agent with dioxide peroxide, realized that liquid nutrient medium does not need also can reach through high temperature, autoclaving the purpose of comprehensive sterilization, not only reduce loaded down with trivial details, complicated technology, also reduced a large amount of power consumptions; Select the high purity photosynthetic bacterium to do provenance; Under gnotobasis, carry out during inoculation, effectively avoided microbial contamination; Photosynthetic bacterium is to be placed on wave and culture on the rotary shaking table when cultivating, and temperature, the illumination regulation and control of photosynthetic bacterium culture environment are effectively regulated and control to reach the purpose that breeds finished product fast.
Used dioxide peroxide among the present invention, add liquid culture matrix according to quantity to after, in 30 minutes, just finish whole sterilization and disinfection process; Again through quiet put 30 hours after, the sterilization and disinfection ability of dioxide peroxide will completely dissolve, at this moment inserts the photosynthetic bacterium bacterial classification, can any injury not arranged to bacterial classification.
The photosynthetic bacterium of the present invention in implementation process is to be placed on wave and culture on the rotary shaking table when cultivating, and the continual photosynthetic bacterium that shakes effective help precipitation of shaking table floats and obtains illumination, the good growth that keeps mycetocyte then.
The present invention is achieved through the following technical solutions:
1, the method for the quick breeding of a kind of high purity photosynthetic bacterium, comprise in the technical scheme: the optimization preparation of liquid nutrient medium, the sterilization method of liquid nutrient medium, selecting for use of culture vessel, the selection of bacterial classification, inoculation and inoculum size under the gnotobasis are cultivated the temperature of environment, illumination regulation and control and culture cycle during cultivation by rotary shaking table.
2, the method for the quick breeding of a kind of high purity photosynthetic bacterium, the various proportioning raw materials of liquid nutrient medium are as follows in the technical scheme:
(1) dipotassium hydrogen phosphate: 120 grams.
(2) sal epsom: 75 grams.
(3) ammonium sulfate: 500 grams.
(4) yeast extract: 500 grams.
(5) peptone: 400 grams.
(6) ferrous sulfate: 50 grams.
(7) sodium-chlor: 120 grams.
(8) sodium hydroxide: an amount of.
(9) water: 100 kilograms.
3, the method for the quick breeding of a kind of high purity photosynthetic bacterium, the preparation of liquid nutrient medium and bottling method are as follows in the technical scheme:
(1) preparation of liquid nutrient medium: after with 10 kilograms 70～80 ℃ hot water above-mentioned raw materials being diluted earlier, sneak into 90 kilograms of surplus water again and stir, follow and namely get liquid nutrient medium after with sodium hydroxide the PH of liquid nutrient medium being adjusted to 7～8.
(2) bottling: the liquid nutrient medium branch is filled to each culture vessel, and Intake Quantity is 2/3 of culture vessel actual capacity.
4, the method for the quick breeding of a kind of high purity photosynthetic bacterium, culture vessel is selected transparent, white vial for use in the technical scheme.
5, the method for the quick breeding of a kind of high purity photosynthetic bacterium, the sterilization method of liquid nutrient medium is as follows in the technical scheme:
(1) be that the stability chlorine dioxide pulvis of 10% dioxide peroxide is made sterilizing agent with content.
(2) add 0.5～1 gram dioxide peroxide pulvis by 1 liter of liquid nutrient medium liquid nutrient medium is carried out disinfection, sterilization is sealed culture vessel with two disposable breathing masks simultaneously.
(3) after liquid nutrient medium is thrown in the dioxide peroxide pulvis according to quantity and carried out disinfection, need quiet putting after 36 hours could insert bacterial classification.
6, the method for the quick breeding of a kind of high purity photosynthetic bacterium is to select color light violet red in the technical scheme, and concentration is that the photosynthetic bacterium finished product of 3,000,000,000/milliliter is done bacterial classification.
7, the method for the quick breeding of a kind of high purity photosynthetic bacterium, method and the inoculum size inoculated in the technical scheme are as follows:
(1) inoculation environment disinfected: liquid nutrient medium and bacterial classification through sterilization are moved in the transfer room (case), and transfer room (case) is carried out disinfection.
(2) inoculation and inoculum size: be 4～5% to be inoculated in the liquid nutrient medium according to inoculum density in the transfer room under gnotobasis (case).
8, the method for the quick breeding of a kind of high purity photosynthetic bacterium is to be placed on rotary shaking table to cultivate when photosynthetic bacterium is cultivated in the technical scheme, and the shaking table speed of rotation is 100～120 rev/mins during cultivation.
9, the method for the quick breeding of a kind of high purity photosynthetic bacterium, temperature, illumination regulation and control and the culture cycle of environment were as follows when photosynthetic bacterium was cultivated in the technical scheme:
(1) culture temperature: the temperature control of culture environment is at 28～36 ℃.
(2) intensity of illumination: be mounted with fluorescent lamp above shaking table, keeping the intensity of illumination of culture environment is 3000～4000 luxs (LX).
(3) culture cycle: photosynthetic bacterium is that 28～36 ℃, intensity of illumination are to cultivate 3～4 days under the environment of 3000～4000 luxs (LX) in temperature, namely breeds into finished product.
Compared with prior art, the existing following advantage of the present invention:
1, simple, the easily enforcement of method technology proposed by the invention.
2, the liquid nutrient medium described in the present invention is through optimizing preparation, and the liquid nutrient medium nutrition-allocated proportion comprehensively, rationally.
3, the present invention's liquid nutrient medium in implementation process is to make sterilizing agent with dioxide peroxide, realized that liquid nutrient medium does not need also can reach through high temperature, autoclaving the purpose of comprehensive sterilization, not only reduce loaded down with trivial details, complicated technology, also reduced a large amount of power consumptions; Be under gnotobasis, to carry out during inoculation, effectively avoided microbial contamination.
Be to be placed on wave and culture on the rotary shaking table when 4, the photosynthetic bacterium of the present invention in implementation process cultivated, the continual photosynthetic bacterium that shakes effective help precipitation of shaking table floats and obtains illumination, the good growth that keeps mycetocyte then.
5, the present invention's temperature, illumination regulation and control to the photosynthetic bacterium culture environment in implementation process effectively regulate and control to reach the purpose that breeds finished product fast, and incubation time has shortened 3～5 days, enhances productivity.
6, the photosynthetic bacterium that cultivates through the present invention all keeps high-quality, high purity, the breeding afterwards of such photosynthetic bacterium enlarges when producing can produce steady quality, photosynthetic bacterium is used in energetic production, also ensures the result of use of producing with photosynthetic bacterium simultaneously.
Embodiment
Below in conjunction with embodiment method of the present invention is further specified.
A kind of high density photosynthetic bacterium is the method for breeding fast, and specific implementation method is as follows:
1, the method for the quick breeding of a kind of high purity photosynthetic bacterium, comprise in the technical scheme: the optimization preparation of liquid nutrient medium, the sterilization method of liquid nutrient medium, selecting for use of culture vessel, the selection of bacterial classification, inoculation and inoculum size under the gnotobasis are cultivated the temperature of environment, illumination regulation and control and culture cycle during cultivation by rotary shaking table.
2, the method for the quick breeding of a kind of high purity photosynthetic bacterium, the various proportioning raw materials of liquid nutrient medium are as follows in the technical scheme:
(1) dipotassium hydrogen phosphate: 120 grams.
(2) sal epsom: 75 grams.
(3) ammonium sulfate: 500 grams.
(4) yeast extract: 500 grams.
(5) peptone: 400 grams.
(6) ferrous sulfate: 50 grams.
(7) sodium-chlor: 120 grams.
(8) sodium hydroxide: an amount of.
(9) water: 100 kilograms.
3, the method for the quick breeding of a kind of high purity photosynthetic bacterium, the preparation of liquid nutrient medium and bottling method are as follows in the technical scheme:
(1) preparation of liquid nutrient medium: after with 10 kilograms 70～80 ℃ hot water above-mentioned raw materials being diluted earlier, sneak into 90 kilograms of surplus water again and stir, follow and namely get liquid nutrient medium after with sodium hydroxide the PH of liquid nutrient medium being adjusted to 7～8.
(2) bottling: the liquid nutrient medium branch is filled to each culture vessel, and Intake Quantity is 2/3 of culture vessel actual capacity.
4, the method for the quick breeding of a kind of high purity photosynthetic bacterium, culture vessel is selected transparent, white vial for use in the technical scheme.
5, the method for the quick breeding of a kind of high purity photosynthetic bacterium, the sterilization method of liquid nutrient medium is as follows in the technical scheme:
(1) be that the stability chlorine dioxide pulvis of 10% dioxide peroxide is made sterilizing agent with content.
(2) add 0.5～1 gram dioxide peroxide pulvis by 1 liter of liquid nutrient medium liquid nutrient medium is carried out disinfection, sterilization is sealed culture vessel with two disposable breathing masks simultaneously.
(3) after liquid nutrient medium is thrown in the dioxide peroxide pulvis according to quantity and carried out disinfection, need quiet putting after 36 hours could insert bacterial classification.
6, the method for the quick breeding of a kind of high purity photosynthetic bacterium is to select color light violet red in the technical scheme, and concentration is that the photosynthetic bacterium finished product of 3,000,000,000/milliliter is done bacterial classification.
7, the method for the quick breeding of a kind of high purity photosynthetic bacterium, method and the inoculum size inoculated in the technical scheme are as follows:
(1) inoculation environment disinfected: liquid nutrient medium and bacterial classification through sterilization are moved in the transfer room (case), and transfer room (case) is carried out disinfection.
(2) inoculation and inoculum size: be 4～5% to be inoculated in the liquid nutrient medium according to inoculum density in the transfer room under gnotobasis (case).
8, the method for the quick breeding of a kind of high purity photosynthetic bacterium is to be placed on rotary shaking table to cultivate when photosynthetic bacterium is cultivated in the technical scheme, and the shaking table speed of rotation is 100～120 rev/mins during cultivation.
9, the method for the quick breeding of a kind of high purity photosynthetic bacterium, temperature, illumination regulation and control and the culture cycle of environment were as follows when photosynthetic bacterium was cultivated in the technical scheme:
(1) culture temperature: the temperature control of culture environment is at 28～36 ℃.
(2) intensity of illumination: be mounted with fluorescent lamp above shaking table, keeping the intensity of illumination of culture environment is 3000～4000 luxs (LX).
(3) culture cycle: photosynthetic bacterium is that 28～36 ℃, intensity of illumination are to cultivate 3～4 days under the environment of 3000～4000 luxs (LX) in temperature, namely breeds into finished product.
Claims (9)
1. the high purity photosynthetic bacterium method of breeding fast, its feature comprises: the optimization preparation of liquid nutrient medium, the sterilization method of liquid nutrient medium, selecting for use of culture vessel, the selection of bacterial classification, inoculation and inoculum size under the gnotobasis are cultivated the temperature of environment, illumination regulation and control and culture cycle during cultivation by rotary shaking table.
2. according to the method for the quick breeding of a kind of high purity photosynthetic bacterium described in the claim 1, it is characterized in that the various proportioning raw materials of liquid nutrient medium are as follows:
(1) dipotassium hydrogen phosphate: 120 grams.
(2) sal epsom: 75 grams.
(3) ammonium sulfate: 500 grams.
(4) yeast extract: 500 grams.
(5) peptone: 400 grams.
(6) ferrous sulfate: 50 grams.
(7) sodium-chlor: 120 grams.
(8) sodium hydroxide: an amount of.
(9) water: 100 kilograms.
3. according to the method for the quick breeding of a kind of high purity photosynthetic bacterium described in the claim 1, it is characterized in that the preparation of liquid nutrient medium and bottling method are as follows:
(1) preparation of liquid nutrient medium: after with 10 kilograms 70～80 ℃ hot water above-mentioned raw materials being diluted earlier, sneak into 90 kilograms of surplus water again and stir, follow and namely get liquid nutrient medium after with sodium hydroxide the PH of liquid nutrient medium being adjusted to 7～8.
(2) bottling: the liquid nutrient medium branch is filled to each culture vessel, and Intake Quantity is 2/3 of culture vessel actual capacity.
4. according to the quick method of breeding of a kind of high purity photosynthetic bacterium described in the claim 1, it is characterized in that culture vessel selects transparent, white vial for use.
5. according to the method for the quick breeding of a kind of high purity photosynthetic bacterium described in the claim 1, it is characterized in that the sterilization method of liquid nutrient medium is as follows:
(1) be that the stability chlorine dioxide pulvis of 10% dioxide peroxide is made sterilizing agent with content.
(2) add 0.5～1 gram dioxide peroxide pulvis by 1 liter of liquid nutrient medium liquid nutrient medium is carried out disinfection, sterilization is sealed culture vessel with two disposable breathing masks simultaneously.
(3) after liquid nutrient medium is thrown in the dioxide peroxide pulvis according to quantity and carried out disinfection, need quiet putting after 36 hours could insert bacterial classification.
6. the method for breeding fast according to a kind of high purity photosynthetic bacterium described in the claim 1 is characterized in that selecting color light violet red, and concentration is that the photosynthetic bacterium finished product of 3,000,000,000/milliliter is done bacterial classification.
7. according to the method for the quick breeding of a kind of high purity photosynthetic bacterium described in the claim 1, it is characterized in that method and the inoculum size inoculated are as follows:
(1) inoculation environment disinfected: liquid nutrient medium and bacterial classification through sterilization are moved in the transfer room (case), and transfer room (case) is carried out disinfection.
(2) inoculation and inoculum size: be 4～5% to be inoculated in the liquid nutrient medium according to inoculum density in the transfer room under gnotobasis (case).
8. the method for breeding fast according to a kind of high purity photosynthetic bacterium described in the claim 1 is characterized in that photosynthetic bacterium is to be placed on rotary shaking table to cultivate when cultivating, and the shaking table speed of rotation is 100～120 rev/mins during cultivation.
9. according to the method for the quick breeding of a kind of high purity photosynthetic bacterium described in the claim 1, it is characterized in that temperature, illumination regulation and control and the culture cycle of environment was as follows when photosynthetic bacterium was cultivated:
(1) culture temperature: the temperature control of culture environment is at 28～36 ℃.
(2) intensity of illumination: be mounted with fluorescent lamp above shaking table, keeping the intensity of illumination of culture environment is 3000～4000 luxs (LX).
(3) culture cycle: photosynthetic bacterium is that 28～36 ℃, intensity of illumination are to cultivate 3～4 days under the environment of 3000～4000 luxs (LX) in temperature, namely breeds into finished product.
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| CN105886430A (en) * | 2016-04-27 | 2016-08-24 | 石家庄大众肥业有限公司 | Method for producing bacillus amyloliquefaciens bacterial agent through solid-state fermentation |
| CN109762723A (en) * | 2019-03-23 | 2019-05-17 | 武汉轻工大学 | A kind of device for photosynthetic bacteria culture |
| CN113943648A (en) * | 2021-11-04 | 2022-01-18 | 吴春蓉 | Anti-pollution type photosynthetic bacteria culture apparatus capable of controlling illumination intensity |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105886430A (en) * | 2016-04-27 | 2016-08-24 | 石家庄大众肥业有限公司 | Method for producing bacillus amyloliquefaciens bacterial agent through solid-state fermentation |
| CN109762723A (en) * | 2019-03-23 | 2019-05-17 | 武汉轻工大学 | A kind of device for photosynthetic bacteria culture |
| CN109762723B (en) * | 2019-03-23 | 2024-04-05 | 武汉轻工大学 | Device for photosynthetic bacteria culture |
| CN113943648A (en) * | 2021-11-04 | 2022-01-18 | 吴春蓉 | Anti-pollution type photosynthetic bacteria culture apparatus capable of controlling illumination intensity |
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| CN103255073B (en) | 2014-12-10 |
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