CN104488969B - A kind of probiotics for suppressing blue-green algae - Google Patents
A kind of probiotics for suppressing blue-green algae Download PDFInfo
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- CN104488969B CN104488969B CN201410612634.2A CN201410612634A CN104488969B CN 104488969 B CN104488969 B CN 104488969B CN 201410612634 A CN201410612634 A CN 201410612634A CN 104488969 B CN104488969 B CN 104488969B
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- green algae
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Abstract
The invention discloses a kind of probiotics for suppressing blue-green algae, the bacterial species that the complete formula of said preparation includes have：Bacillus subtilis (Bacillus subtilis), Rhodopseudomonas palustris (Rhodop seudanonas palustris), sulfide-oxidizing enzyme (Thiobacillus.sp), Bacillus acidi lactici (Lactobacillus.sp) and Composite Photosynthetic bacterium (Photosynthetic bacteria abbr).Said preparation can effectively suppress blue algae growth, and can efficiently control the content of ammonia nitrogen and hydrogen sulfide, significantly improve the content of dissolved oxygen, improve water quality, improve culture benefit.
Description
Technical field
The present invention relates to water purification technology field, more particularly to a kind of probiotics for suppressing blue-green algae.
Background technology
Blue-green algae is prokaryotes, is called blue-green alge, cyanobacteria, including basketball algae, the algae that quivers, nostoc, Microcystis aeruginosa etc..Distribution
It is quite varied, various regions all over the world, in culture environment of aquatic products and eutrophy natural water area, often in summer amount reproduction, in water
Face forms one layer of blue-green and has the offscum of bad smell, is referred to as " wawter bloom ", so as to cause water quality deterioration, water oxygen is exhausted when serious
Gas causes the aquatic animals such as fish shrimp crab dead.More seriously, some species (such as Microcystis aeruginosa) can also produce toxin in blue-green algae
(MC), domestic animal and wild animal have been drunk after the water containing Algae toxins, it may appear that diarrhoea, weak, apocleisis, vomiting, drowsiness, mouth eye point
The symptoms such as secretions, in addition it is dead.People have a bath, swimming when, skin contact water body containing Algae toxins can cause as eyes with
It is allergic, drink into acute gastroenteritis can be caused, drink for a long time on a small quantity, liver cancer may be triggered.Medical research has found drinking-water
In micro Microcystin and the incidence of disease of primary carcinoma of liver in crowd have very big correlation.
With continuing to develop for culture fishery, fisherman covets pond high yield, to make cultivation benefit, constantly
Increase cultivation density, increase the input of feed, pond facility aging, water body overburden production causes body eutrophication.In richness
In nutritive water, the breeding of blue-green algae rapid, high volume forms cyanobacterial bloom, and wawter bloom occurs that the breeding of other algae can be suppressed, together
When intraspecific competition cause blue-green algae mortality and decomposition, cause the dissolved oxygen in water to decline rapidly, while produce azanol, hydrogen sulfide
Deng noxious material.Fishes and shrimps etc. cultivate object because anoxic is raised the nose above water to breathe and azanol, hydrogen sulfide poisoning, final dead, bring huge to aquaculture
Big loss.Current aquaculture mainly using plant ash uniform fold is shed, controls illumination, algae natural death is sunk；Or
Person uses herbicide (such as prometryn), copper sulphate.But effect is not good, and easily produce secondary pollution.
The content of the invention
The probiotics of blue-green algae can be suppressed it is an object of the invention to provide a kind of, while dead algae can be avoided
Water body ammonia nitrogen, content of nitrite is caused to improve, the secondary pollution caused.
The technical solution used in the present invention is：
The probiotics is made up of following raw materials：Bacillus subtilis (Bacillus subtilis), marsh are red false single
Born of the same parents bacterium (Rhodop seudanonas palustris), sulfide-oxidizing enzyme (Thiobacillus.sp), Bacillus acidi lactici
(Lactobacillus.sp) and Composite Photosynthetic bacterium (Photosynthetic bacteriaabbr), each bacterium is according to mass ratio
Example is added, and its adding proportion is 1:1:1:1:1.
It is preferred that, overall viable bacteria content is 6 × 10 in the probiotics for suppressing blue-green algae of the invention8-8×109Individual/milli
Rise.
Blue algae growth can effectively be suppressed by suppressing the probiotics of blue-green algae, and can degradation of ammonia nitrogen, the content of hydrogen sulfide, control
Dissolved oxygen processed, improves water quality.
It is preferred that, the probiotics for suppressing blue-green algae is liquid, is conducive to uniformly disseminating in water body.
It is preferred that, the probiotics application method for suppressing blue-green algae is that in the pond of one meter of depth, one mu of pool uses this
The probiotics of invention is 1000-1200 milliliters.
The beneficial effects of the present invention are：The probiotics worked good is delivered according to application method and arrives cultivating pool
In, can effectively suppress the growth of blue-green algae, and can degradation of ammonia nitrogen, the content of hydrogen sulfide, add the dissolved oxygen amount of breeding water body,
Water quality is improved, the yield of cultivation fish is improved.
Embodiment
With reference to embodiments of the invention, clear and detailed retouch is carried out to the technical scheme in the embodiment of the present invention
State, it is clear that described embodiment is only a part of embodiment of the present invention, rather than whole embodiments.Based on the present invention
In embodiment, all other implementation that those of ordinary skill in the art are obtained under the premise of creative work is not made
Example, belongs to protection scope of the present invention.
Below the present invention is further illustrated with embodiment.
Embodiment 1
Specific preparation method includes following steps：
1st, scientific compatibility goes out the higher composite functional bacterial strain of performance
According to the different strains to selection：Bacillus subtilis (Bacillus subtilis), Rhodopseudomonas palustris
(Rhodop seudanonas palustris), sulfide-oxidizing enzyme (Thiobacillus.sp), Bacillus acidi lactici
(Lactobacillus.sp) and Composite Photosynthetic bacterium (Photosynthetic bacteriaabbr) functional study, combination
Go out different composite bacteria agents.With water based on the water of blue-green alga bloom (based on the algae that quivers, Microcystis aeruginosa), existed with different bacterial strain combinations
Under the conditions of difference culture salinity, different cultivation temperature, the content of the water body Determination of Chlorophyll a using after 72h, total nitrogen, total phosphorus content as
Testing index does compbined test, obtains best of breed.
2nd, the preparation of probiotics
Aseptically, the strain of the best of breed of gained is combined, the ratio of each bacterial strain quality is 1:
1:1:1:1, preparation is made by carrying out liquid fermentation respectively, then carry out sterile filling.
Embodiment 2：Saline-alkali water application test
Test period：On October 20th, 2010 to October 23.
Test site：Yanshan County Ma Cun
Test method and process：Choose two culturing pools, one is experiment deck label 1#, one be control group marked as
2#.1# test tanks, that morning uses the microbial inoculum, and consumption is that every mu (1 meter of depth of water) uses 1000mL, and usage is full pond after dilution
Splash, leeward suitably more spills, while opening aerator.2# is control pond, and that morning uses copper sulphate, and consumption is every mu (1
500mL meter Shui Shen) is used, usage is full pool spilling head after dilution, and leeward suitably more spills, while opening aerator.Experimental result
It is shown in Table 1,2.
The experiment change in the 1# ponds of table 1
The experiment change in the 2# ponds of table 2
Result of the test shows that the microbial inoculum can effectively suppress the growth of blue-green algae, and the strict control water body pH of energy, ammonia
Nitrogen, hydrogen sulfide, dissolved oxygen.Although copper sulphate can be good at suppressing blue-green algae, secondary pollution is easily caused, by contrast,
Said preparation has overwhelming superiority.
Embodiment 3：Fresh water application test
Test period：August 6 to 8 in 2009.
Test site：The Li Lixin plants of Tangshan, Hebei Province Tanghai County 11
Test method and process：Choose two culturing pools, one is experiment deck label 1#, one be control group marked as
2#.1# test tanks, that morning uses said preparation, and consumption is that every mu (1 meter of depth of water) uses 1000mL, and usage is full pond after dilution
Splash, leeward suitably more spills, while opening aerator.2# is control pond, and that morning uses copper sulphate, and consumption is every mu (1
500mL meter Shui Shen) is used, usage is full pool spilling head after dilution, and leeward suitably more spills, while opening aerator.Experimental result
It is shown in Table 3.
The fresh water application test result of table 3
Fresh water application test result is same it can be shown that said preparation more has application than conventional chemical reagent copper sulphate,
It can be good at controlling the amount of the harmful substances such as ammonia nitrogen, hydrogen sulfide while the quantity of blue-green algae is efficiently controlled.
The embodiment of each in this specification is described by the way of progressive, and what each embodiment was stressed is and other
Between the difference of embodiment, each embodiment identical similar portion mutually referring to.
The foregoing description of the disclosed embodiments, enables professional and technical personnel in the field to realize or using the present invention.
A variety of modifications to these embodiments are it will be apparent that as defined herein one for those skilled in the art
As principle can realize in other embodiments without departing from the spirit or scope of the present invention.Therefore, the present invention will
It will not be intended to be limited to the embodiments shown herein, and be to fit to consistent with principles disclosed herein and features of novelty
Most wide scope.
Claims (4)
1. a kind of probiotics for suppressing blue-green algae, it is characterised in that：It is made up of following raw materials：Bacillus subtilis
(Bacillus subtilis), Rhodopseudomonas palustris (Rhodop seudanonas palustris), sulfide-oxidizing enzyme
(Thiobacillus.sp), Bacillus acidi lactici (Lactobacillus.sp) and Composite Photosynthetic bacterium (Photosynthetic
Bacteriaabbr), the adding proportion of each bacterium is 1:1:1:1:1.
2. the probiotics according to claim 1 for suppressing blue-green algae, it is characterised in that：The Tiny ecosystem for suppressing blue-green algae
Overall viable bacteria content is 6 × 10 in preparation8-8×109Individual/milliliter.
3. the probiotics according to claim 1 for suppressing blue-green algae, it is characterised in that：The Tiny ecosystem system of the suppression blue-green algae
Agent is liquid.
4. the probiotics according to claim 1 for suppressing blue-green algae, it is characterised in that：The Tiny ecosystem system of the suppression blue-green algae
Agent application method is 1000-1200 milliliters/mu rice.
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| CN104488969B true CN104488969B (en) | 2017-10-03 |
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Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106472599A (en) * | 2016-09-22 | 2017-03-08 | 匡新生 | A kind of blue algae growth inhibitor |
| CN108249588A (en) * | 2018-02-08 | 2018-07-06 | 上海山恒生态科技股份有限公司 | A kind of composite bacteria agent formula for reducing water body ammonia-nitrogen content |
| CN110438045A (en) * | 2019-08-21 | 2019-11-12 | 江西清瀞自然环境科技有限公司 | A kind of compound micro-ecological preparation and preparation method thereof of lake ecological control algae |
| CN113461179A (en) * | 2021-08-06 | 2021-10-01 | 南京农业大学 | Application of rhodopseudomonas palustris ZT-MG2 in eutrophic water body treatment |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101078003A (en) * | 2006-05-23 | 2007-11-28 | 上海创博生态工程有限公司 | Preparation for comprehensive treatment of cultivation water body by using multiple bacterial and manufacturing method thereof |
| CN101629157A (en) * | 2009-08-06 | 2010-01-20 | 江苏省江大绿康生物工程技术研究有限公司 | Compound microecologics for purifying water quality |
| CN101712511A (en) * | 2009-04-21 | 2010-05-26 | 北京普仁生态技术有限公司 | Method for controlling cyanobacterial bloom by utilizing composite strain and trace element |
| CN102531198A (en) * | 2012-01-09 | 2012-07-04 | 信息产业电子第十一设计研究院科技工程股份有限公司 | Water body cleansing agent |
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- 2014-11-03 CN CN201410612634.2A patent/CN104488969B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101078003A (en) * | 2006-05-23 | 2007-11-28 | 上海创博生态工程有限公司 | Preparation for comprehensive treatment of cultivation water body by using multiple bacterial and manufacturing method thereof |
| CN101712511A (en) * | 2009-04-21 | 2010-05-26 | 北京普仁生态技术有限公司 | Method for controlling cyanobacterial bloom by utilizing composite strain and trace element |
| CN101629157A (en) * | 2009-08-06 | 2010-01-20 | 江苏省江大绿康生物工程技术研究有限公司 | Compound microecologics for purifying water quality |
| CN102531198A (en) * | 2012-01-09 | 2012-07-04 | 信息产业电子第十一设计研究院科技工程股份有限公司 | Water body cleansing agent |
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Inventor after: Zhang Lianshui Inventor after: Wang Chunguo Inventor after: Song Jian Inventor after: Gao Caiquan Inventor after: Zhang Jin Inventor after: Zhang Wanglin Inventor after: Meng Huixian Inventor after: Zhang Qingsong Inventor after: Song Xuezhang Inventor after: Li Chunling Inventor after: Li Wenmin Inventor after: Zhang Jun Inventor after: Sun Yueshuang Inventor after: Wang Jifen Inventor after: Zhang Zhiying Inventor before: Zhang Lianshui |
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