CN104488969A - Microecological preparation for inhibiting blue-green algae - Google Patents

Microecological preparation for inhibiting blue-green algae Download PDF

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Publication number
CN104488969A
CN104488969A CN201410612634.2A CN201410612634A CN104488969A CN 104488969 A CN104488969 A CN 104488969A CN 201410612634 A CN201410612634 A CN 201410612634A CN 104488969 A CN104488969 A CN 104488969A
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green algae
blue
probiotics
preparation
water
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CN104488969B (en
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张连水
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CANGZHOU WANGFA BIOTECHNOLOGY INSTITUTE Co Ltd
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CANGZHOU WANGFA BIOTECHNOLOGY INSTITUTE Co Ltd
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Abstract

The invention discloses a microecological preparation for inhibiting blue-green algae. The complete formula of the preparation comprises the following bacterium kinds: bacillus subtilis, rhodop seudanonas palustris, thiobacillus.sp, lactobacillus.sp and photosynthetic bacteria abbr. The preparation is capable of effectively inhibiting growth of blue-green algae, is capable of effectively controlling the content of ammonia nitrogen and hydrogen sulfide, and helps to substantially improve the dissolved oxygen content, improve water quality and improve culture benefit.

Description

A kind of probiotics suppressing blue-green algae
Technical field
The present invention relates to water purification technology field, particularly relate to a kind of probiotics suppressing blue-green algae.
Background technology
Blue-green algae is prokaryotes, cries again blue-green alge, cyanobacteria, comprise basketball algae, the algae that quivers, nostoc, Microcystis aeruginosa etc.Be widely distributed, various places all over the world, in culture environment of aquatic products and eutrophy natural water area, normal in amount reproduction in summer, form one deck blue-green at the water surface and have the offscum of bad smell, be called " wawter bloom ", thus cause water quality deterioration, exhaust water oxygen gas time serious and cause the aquatic animals such as fish shrimp crab dead.More seriously, in blue-green algae, some kind (as Microcystis aeruginosa) also can produce toxin (MC), after domestic animal and wildlife have drunk the water containing Algae toxins, symptom, the even death such as there will be diarrhoea, weak, apocleisis, vomiting, drowsiness, mouth discharge of eye increases.People are when having a bath, swimming, and skin contact can cause as eyes and allergic containing Algae toxins water body, and drink on a small quantity into causing acute gastroenteritis, long-term drinking then may cause liver cancer.During medical research has found to drink water, in micro-Microcystin and crowd, the incidence of disease of primary carcinoma of liver has very large correlation.
Along with the development of culture fishery, fisherman covets pond high yield, for making cultivation benefit, constantly increases cultivation density, and increase the input of feed, pond facility is aging, and water body overburden is produced, and causes body eutrophication.In eutrophication water, blue-green algae rapid, high volume is bred, and form cyanobacterial bloom, wawter bloom appearance can suppress the breeding of other algae, intraspecific competition simultaneously causes blue-green algae mortality Sum decomposition, causes the dissolved oxygen in water to decline rapidly, produces the noxious material such as azanol, hydrogen sulphide simultaneously.The cultivation such as fishes and shrimps object is because of anoxic floating head and azanol, hydrogen sulfide poisoning, finally dead, carrys out massive losses to cultivation industrial belt.Current aquaculture mainly adopts sheds ash uniform fold, controlled light, and algae natural death is sunk; Or use weed killer herbicide (as prometryn), copper sulphate.But poor effect, and easily produce secondary pollution.
Summary of the invention
The object of this invention is to provide a kind of probiotics that can suppress blue-green algae, dead algae can have been avoided to cause water body ammonia nitrogen, content of nitrite to improve, the secondary pollution caused simultaneously.
The technical solution used in the present invention is:
This probiotics is made up of following raw material: bacillus subtilis (Bacillus subtilis), Rhodopseudomonas palustris (Rhodop seudanonas palustris), sulfide-oxidizing enzyme (Thiobacillus.sp), Bacillus acidi lactici (Lactobacillus.sp) and Composite Photosynthetic bacterium (Photosynthetic bacteriaabbr), each bacterium is added according to mass ratio, and its adding proportion is 1:1:1:1:1.
Preferably, in the probiotics of suppression blue-green algae of the present invention, overall viable bacteria content is 6 × 10 8-8 × 10 9individual/milliliter.
Suppress the probiotics of blue-green algae effectively can suppress blue algae growth, and the content of energy degradation of ammonia nitrogen, hydrogen sulphide, control dissolved oxygen, improve water quality.
Preferably, the probiotics suppressing blue-green algae is liquid, is conducive to disseminating uniformly in water body.
Preferably, the probiotics using method of blue-green algae is suppressed to be that one mu of pool uses probiotics of the present invention for 1000-1200 milliliter in one meter of dark pond.
Beneficial effect of the present invention is: render in cultivating pool by the probiotics of working good according to using method, effectively can suppress the growth of blue-green algae, and can degradation of ammonia nitrogen, hydrogen sulphide content, add the dissolved oxygen amount of breeding water body, improve water quality, improve the output of cultivation fish.
Embodiment
Below in conjunction with embodiments of the invention, carry out clear and detailed description to the technical scheme in the embodiment of the present invention, obviously, described embodiment is only a part of embodiment of the present invention, instead of whole embodiment.Based on the embodiment in the present invention, those of ordinary skill in the art, not making other embodiments all obtained under creative work prerequisite, belong to protection scope of the present invention.
The present invention is further illustrated below by embodiment.
Embodiment 1
Concrete preparation method comprises following step:
1, scientific compatibility goes out the higher composite functional bacterial strain of performance
According to the different strains selected: the functional study of bacillus subtilis (Bacillus subtilis), Rhodopseudomonas palustris (Rhodop seudanonas palustris), sulfide-oxidizing enzyme (Thiobacillus.sp), Bacillus acidi lactici (Lactobacillus.sp) and Composite Photosynthetic bacterium (Photosynthetic bacteriaabbr), is combined into different composite bacteria agent capables.With water based on the water of blue-green alga bloom (based on the algae that quivers, Microcystis aeruginosa), under being combined in different cultivation salinity, different cultivation temperature condition with different bacterial strains, with the content of the content of water body Determination of Chlorophyll a after 72h, total nitrogen, total phosphorus for testing index does complex experiment, obtain best of breed.
2, the preparation of probiotics
Aseptically, combined by the bacterial classification of the best of breed of gained, the ratio of each bacterial strain quality is 1:1:1:1:1, makes preparation, then carry out sterile filling through carrying out liquid fermentation respectively.
Embodiment 2: saline-alkali water application test
Test period: on October 20th, 2010 was to October 23.
Test site: Yanshan County Ma Cun
Test method and process: choose two culturing pools, one is experimental group label 1#, one for control group label be 2#.1# test tank, that morning uses this microbial inoculum, and consumption is that every mu (1 meter of depth of water) uses 1000mL, and usage is full pool spilling head after dilution, and leeward suitably more spills, and opens aerator simultaneously.2# is contrast pond, and that morning uses copper sulphate, and consumption is that every mu (1 meter of depth of water) uses 500mL, and usage is full pool spilling head after dilution, and leeward suitably more spills, and opens aerator simultaneously.Experimental result is in table 1,2.
The test change in table 1 1# pond
The test change in table 2 2# pond
Result of the test shows, this microbial inoculum effectively can suppress the growth of blue-green algae, and control water body pH, ammonia nitrogen, hydrogen sulphide, dissolved oxygen that can be strict.Although copper sulphate can be good at suppressing blue-green algae, easily cause secondary pollution, by contrast, said preparation has overwhelming superiority.
Embodiment 3: fresh water application test
Test period: 6 to 8 August in 2009.
Test site: 11, Tanghai County, Tangshan, Hebei Province Li Lixin plant
Test method and process: choose two culturing pools, one is experimental group label 1#, one for control group label be 2#.1# test tank, that morning uses said preparation, and consumption is that every mu (1 meter of depth of water) uses 1000mL, and usage is full pool spilling head after dilution, and leeward suitably more spills, and opens aerator simultaneously.2# is contrast pond, and that morning uses copper sulphate, and consumption is that every mu (1 meter of depth of water) uses 500mL, and usage is full pool spilling head after dilution, and leeward suitably more spills, and opens aerator simultaneously.Experimental result is in table 3.
Table 3 fresh water application test result
Fresh water application test result can show equally, and said preparation more has application than conventional chemical reagent copper sulphate, can be good at the amount controlling the harmful substance such as ammonia nitrogen, hydrogen sulphide while the quantity effectively controlling blue-green algae.
In this specification, each embodiment adopts the mode of going forward one by one to describe, and what each embodiment stressed is the difference with other embodiments, between each embodiment identical similar portion mutually see.
To the above-mentioned explanation of the disclosed embodiments, professional and technical personnel in the field are realized or uses the present invention.Be apparent for those skilled in the art to the multiple amendment of these embodiments, General Principle as defined herein can without departing from the spirit or scope of the present invention, realize in other embodiments.Therefore, the present invention can not be restricted to these embodiments shown in this article, but will meet the widest scope consistent with principle disclosed herein and features of novelty.

Claims (4)

1. one kind is suppressed the probiotics of blue-green algae, it is characterized in that: be made up of following raw material: bacillus subtilis (Bacillus subtilis), Rhodopseudomonas palustris (Rhodop seudanonas palustris), sulfide-oxidizing enzyme (Thiobacillus.sp), Bacillus acidi lactici (Lactobacillus.sp) and Composite Photosynthetic bacterium (Photosyntheticbacteriaabbr), the adding proportion of each bacterium is 1:1:1:1:1.
2. the probiotics of suppression blue-green algae according to claim 1, is characterized in that: in the probiotics of described suppression blue-green algae, overall viable bacteria content is 6 × 10 8-8 × 10 9individual/milliliter.
3. the probiotics of suppression blue-green algae according to claim 1, is characterized in that: the probiotics of this suppression blue-green algae is liquid.
4. the probiotics of suppression blue-green algae according to claim 1, is characterized in that: the probiotics using method of this suppression blue-green algae is 1000-1200 milliliter/mu rice.
CN201410612634.2A 2014-11-03 2014-11-03 A kind of probiotics for suppressing blue-green algae Active CN104488969B (en)

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106472599A (en) * 2016-09-22 2017-03-08 匡新生 A kind of blue algae growth inhibitor
CN108249588A (en) * 2018-02-08 2018-07-06 上海山恒生态科技股份有限公司 A kind of composite bacteria agent formula for reducing water body ammonia-nitrogen content
CN110438045A (en) * 2019-08-21 2019-11-12 江西清瀞自然环境科技有限公司 A kind of compound micro-ecological preparation and preparation method thereof of lake ecological control algae
CN113461179A (en) * 2021-08-06 2021-10-01 南京农业大学 Application of rhodopseudomonas palustris ZT-MG2 in eutrophic water body treatment

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CN101629157A (en) * 2009-08-06 2010-01-20 江苏省江大绿康生物工程技术研究有限公司 Compound microecologics for purifying water quality
CN101712511A (en) * 2009-04-21 2010-05-26 北京普仁生态技术有限公司 Method for controlling cyanobacterial bloom by utilizing composite strain and trace element
CN102531198A (en) * 2012-01-09 2012-07-04 信息产业电子第十一设计研究院科技工程股份有限公司 Water body cleansing agent

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CN101078003A (en) * 2006-05-23 2007-11-28 上海创博生态工程有限公司 Preparation for comprehensive treatment of cultivation water body by using multiple bacterial and manufacturing method thereof
CN101712511A (en) * 2009-04-21 2010-05-26 北京普仁生态技术有限公司 Method for controlling cyanobacterial bloom by utilizing composite strain and trace element
CN101629157A (en) * 2009-08-06 2010-01-20 江苏省江大绿康生物工程技术研究有限公司 Compound microecologics for purifying water quality
CN102531198A (en) * 2012-01-09 2012-07-04 信息产业电子第十一设计研究院科技工程股份有限公司 Water body cleansing agent

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106472599A (en) * 2016-09-22 2017-03-08 匡新生 A kind of blue algae growth inhibitor
CN108249588A (en) * 2018-02-08 2018-07-06 上海山恒生态科技股份有限公司 A kind of composite bacteria agent formula for reducing water body ammonia-nitrogen content
CN110438045A (en) * 2019-08-21 2019-11-12 江西清瀞自然环境科技有限公司 A kind of compound micro-ecological preparation and preparation method thereof of lake ecological control algae
CN113461179A (en) * 2021-08-06 2021-10-01 南京农业大学 Application of rhodopseudomonas palustris ZT-MG2 in eutrophic water body treatment

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