CN106465695A - A kind of enhanced ecological base and preparation method thereof - Google Patents
A kind of enhanced ecological base and preparation method thereof Download PDFInfo
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K63/00—Receptacles for live fish, e.g. aquaria; Terraria
- A01K63/04—Arrangements for treating water specially adapted to receptacles for live fish
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2305/00—Use of specific compounds during water treatment
- C02F2305/06—Nutrients for stimulating the growth of microorganisms
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W10/00—Technologies for wastewater treatment
- Y02W10/30—Wastewater or sewage treatment systems using renewable energies
- Y02W10/37—Wastewater or sewage treatment systems using renewable energies using solar energy
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Microbiology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Biodiversity & Conservation Biology (AREA)
- Animal Husbandry (AREA)
- Marine Sciences & Fisheries (AREA)
- Hydrology & Water Resources (AREA)
- Engineering & Computer Science (AREA)
- Environmental & Geological Engineering (AREA)
- Water Supply & Treatment (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
Abstract
The present invention relates to a kind of ecological matrix, a kind of specifically related to enhanced ecological matrix of property processing and preparation method thereof, ecological matrix is processed using the solution comprising carbon source and/or microorganism formulation solution, obtain after drying, the described carbon source solution that comprises is the solution being substantially free of nitrogen source, and described microorganism formulation is the preparation obtaining after one or more of bacillus cereuss, photosynthetic bacteria, lactic acid bacteria ferment.The enhanced ecological base preparation cost of the present invention is low, method is simple to operate and easy realization, and the enhanced ecological base preparing, it is experimentally verified that, notable shortening biofilm time, repairing effect of increasing water quality, the speed of growth improving Fish and the incidence rate reducing disease, there is very big economic worth and practical function, greatly improve the water treatment efficiency of existing ecological matrix using simple and cheap method.
Description
Technical field
The present invention relates to a kind of ecological matrix is and in particular to a kind of enhanced ecological matrix of property processing and its preparation side
Method.
Background technology
Culture fishery is that human use is available for cultivating the waters of (include plantation), according to cultivation object ecological habit and
Requirement to water environment condition, with aquaculture technology and facility, is engaged in aquatic economic animal and plant cultivation.
China is to be engaged in one of aquaculture country with the longest history in the world, and cultivation is experienced, and cultural technique is general
And.6699.65 ten thousand tons of China Water product total output in 2016, increases by 3.69% than last year.Wherein, cultured output 4937.90 ten thousand
Ton, accounts for the 73.70% of total output, increases by 3.99% on a year-on-year basis.Aquatic products industry is in the national economy of China, particularly agro based economic development
In occupy increasingly consequence.
Aquatic products are the important sources of mankind's high-quality protein, and it is rich in high-quality protein, aminoacid, vitamin and mineral
Deng, and quantity and ratio meet human body needs, particularly containing the larger leucine of human body requirements amount and lysine.Aquatic products
In connective tissue content few more than poultry meat, fish muscular fiber is shorter, and proteinaceous tissue is loose, and moisture is high, easily by people
Body is digested and assimilated.Therefore, with the raising of people's income level, increasing Chinese start using nutritional need as food
The primary need of consumption, it is trend of the times that the consumption proportion of aquatic products rises.
With developing rapidly of Chinese Fishery aquaculture, aquaculture total amount occupies the first in the world for years.Same with this
When, culture fishery by traditional low-density, extensive style, low input and low yield exit pattern, to high density, intensive, high
Put into and high production Mode change.Aquaculture is providing a large amount of high-quality protein sources, is creating huge economic value for people
Meanwhile, also bring the environmental problem that cannot ignore.The especially a large amount of discharge of breeding wastewater leads to coastal waters red tide frequently quick-fried
Send out, have a strong impact on the balance of Marginal Sea Ecosystems and stablize, also govern the sustainable health development of culture fishery.
Aquaculture system belongs to eutrophic aquatic ecosystem, due to generation and the decomposition of residual bait and fish and shrimp Excreta
Deng making breeding water body be rich in various dissolubility nutrient substance and organic suspended matter.Due to breeding water body ecosystem structure list
One, make the residual bait in cultivating system, the material circulation of fish and shrimp Excreta and energy Flow become a kind of cumulative partial circulating, water
Body develops to the direction of eutrophication rapidly, causes the decomposer in water body and Producer to turn to adapting to the species in pollution environment
Become, bio-diversity declines, thus threatening the living environment of cultivated animals.If the breeding wastewater that will be enriched in nutritive salt is arranged in a large number
Put, then can cause surface water body eutrophication, when the water body of this eutrophication has suitable biology, the hydrology and meteorological condition
When it is possible to produce red tide or wawter bloom, cause water pollution.As can be seen here, the eutrophic state improving breeding water body becomes
Realize the key issue of healthy aquaculture.
Ecological matrix technology is a kind of purification of water quality treatment technology that is theoretical based on bio-diversity and growing up.Ecological matrix
Technology exactly utilizes bio-diversity principle, assembles antibacterial in water body, funguses, algae, protozoacide and metazoa etc.,
Form a kind of stable Tiny ecosystem system of complexity in water body.Once ecological matrix is put in breeding water body system, carrier surface is permissible
Attached microbial, plankton and protozoacide etc., and aufwuch quantity is continuously increased, these biologies by itself
Organic substance in metabolism decomposition water, the eutrophy in biology (such as microorganism) the adsorbable water body of growth on ecological matrix becomes
Point, the such as material such as nitrogen phosphate and sulfur, carbon, and these eutrophy are become separating/enriching, by different biological metabolism effects, transform into
Carbon dioxide, water and nitrogen etc., and then purifying aquaculture waters environment, reach the effect of regulating water quality.
But, in actual applications, there is following significantly shortcoming in ecological matrix：
(1) the start-up time is long, and in cultivating system, ecological matrix natural membrane maturation typically requires 30 days -40 days even
Longer, this has become the bottleneck of ecological matrix application in main cultivating system；
(2) water treatment efficiency of startup stage is low, and the abundance that ecological matrix puts into water body early stage attached microbial is not enough, raw
State base attached microbial requires time for process, will not play the effect of water quality adjustment at once, and this directly affects the operation of ecological matrix
Effect；
(3) pollutants removal rate lifting is slow, reason as noted above, ecological matrix put into water body early stage biofilm slow and
Bacterial abundance is not enough, and early stage pollutant removal is poor, elapses although pollutant removal has lifting over time, but
It is an up effect slow.
For improving the water quality Self-controlled ability of cultivating system, optimization processing further to ecological matrix in need, to lift life
The using effect of state base.
Content of the invention
For above deficiency, the present invention provides a kind of biofilm time short, the high enhanced ecological base of Water quality restoration effect and
Its preparation method, processes breeding water body using this ecological matrix, the speed of growth of Fish improves and disease incidence reduces.
In a first aspect, the present invention provide a kind of enhanced ecological base, using the solution comprising carbon source and/or microorganism system
Agent solution processes ecological matrix, is strengthened type ecological matrix after being dried, and the described carbon source solution that comprises is be substantially free of nitrogen source molten
Liquid, described microorganism formulation is the preparation obtaining after one or more of bacillus cereuss, photosynthetic bacteria, lactic acid bacteria ferment.
In second aspect, the present invention provides a kind of enhanced ecological base preparing using the method for the present invention.
In the third aspect, the present invention provides a kind of using method in breeding water body of enhanced ecological base.
In fourth aspect, the present invention provides a kind of purposes in water process for enhanced ecological base.
Biofilm maturation time in breeding water body for the enhanced ecological base of the present invention shortens 30-50%, and Water quality restoration is imitated
Rate improves 10%-20%, and fish growth speed improves more than 10%, and disease incidence reduces by more than 20%.
The enhanced ecological base preparation cost of the present invention is low, and method is simple to operate and easy realization, and prepare
Enhanced ecological base, is experimentally verified that, the notable shortening biofilm time, and repairing effect of increasing water quality improves the speed of growth of Fish
And reduce the incidence rate of disease, there is very big economic worth and practical function, great using simple and cheap method
Improve the water treatment efficiency of existing ecological matrix.
Specific embodiment
In detail below the present invention is further described.
Researcher finds, although existing ecological matrix shows certain effect in water process, however, because it is intrinsic
Characteristic and the biofilm time length that leads to, startup stage water treatment efficiency are low and pollutants removal rate lifting the is slow very big ground resistance of defect
Hinder the fast development of ecological matrix and its use, the researcher being devoted to solving the above-mentioned restraining factors of ecological matrix finds, improves life
The performance of state base is an extremely complex job, the complexity of operation and cost, is all that enhanced type is prepared in impact restriction
The factor of ecological matrix, at present, does not also have a kind of specifically simple and cheap, method that ecological matrix performance can be greatly improved.Though
So, prior art is always catching at breakthrough with regard to ecological matrix aspect of performance, even, there is part research to be centered around ecological matrix and use
During how rapid biofilm aspect is carried out, but, on the one hand, the raising effect during use is not notable, on the other hand,
Rapid biofilm method during use, is difficult to promote and unified, and due to environment and personnel's impact of operation, effect deviation is relatively
Greatly.
The research worker of the present invention carries out research and finds, in breeding environment, the environment of high nitrogen (N) low-carbon (LC) (C) may imply that
Nutrient substance (particularly carbon) in cultivation water environment and on ecological matrix lacks it is impossible to supply growth of microorganism and the anti-nitre of denitrogenation
Change the electron donor of (being N2 by nitrite and nitrate transformation, thus the N of water body is discharged into the atmosphere), thus affecting to give birth to
The effect of state base.This is because, the most of C putting in cultivating system can be with CO under microbial metabolism effect2Form
Discharge from pond environment, and N is only under anaerobic on a small quantity with N2、N2The form release of O, most N can be with inorganic
The form of nitrogen is stayed in breeding water body, leads to breeding environment system C/N than low.
Further study showed that, carbon source and the bacteria preparation rich in nutrient substance in speed-raising biomembrane film forming and improve anti-
All there is certain facilitation, both of which can provide certain carbon source to supply ecological matrix attached microbial on nitrification denitrification effect
Growth demand, can also supply microorganism denitrifying electron donor simultaneously, complete the process of the denitrification denitrogenation of sewage disposal.
1st, enhanced ecological base preparation method
Find, the present invention proposes a kind of method preparing enhanced ecological base based on above research, using comprising carbon source
Solution and/or microorganism formulation solution process ecological matrix, be dried after obtain, comprising carbon source solution is to be substantially free of nitrogen source
Solution, microorganism formulation is the preparation obtaining after one or more of bacillus cereuss, photosynthetic bacteria, lactic acid bacteria ferment.
The ecological matrix that the solution that the enhanced ecological base of the present invention can include comprising carbon source is processed, or adopt microorganism system
Ecological matrix that agent solution is processed etc..
In a preferred embodiment, the solution comprising carbon source is comprising in carbohydrate carbon source or biomass carbon source
Plant or multiple solution.
Specifically, the solution comprising carbon source can be the solution of the mixture of one or more of carbohydrate carbon source, also may be used
Think the solution of the mixture of one or more of biomass carbon source, can also be selected from one or more of carbohydrate carbon source
Carbon source and selected from one or more of biomass carbon source carbon source, the solution of the mixture of composition.
In a preferred embodiment, carbohydrate carbon source is selected from the mixed of one or more of sucrose, glucose, starch
Compound.
In a preferred embodiment, biomass carbon source is in cellobiose, mannose, xylan, galactose
One or more of mixture.
In a preferred embodiment, the solution comprising carbon source is the solution substantially only comprising carbon source.
In a preferred embodiment, the concentration range comprising the solution of carbon source is 10mg/L～40mg/L, enters one
Step is preferably 20mg/L～25mg/L.
In a preferred embodiment, carbon source can separately be selected from：Sucrose；Or the mixing of sugarcane sugar and starch
Thing (such as 3:1 weight ratio)；Or cellobiose；Or the mixture (such as 1 of cellobiose and mannose:1 weight ratio)；Or
The mixture (such as 1 of sucrose and cellobiose：2 weight ratio) etc..
In a further preferred embodiment, the solution comprising carbon source is the sucrose solution of 20mg/L.
In a further preferred embodiment, comprise the sugarcane sugar and starch that the solution of carbon source is 22.5mg/L for concentration
The solution of mixture, the weight of sugarcane sugar and starch is than for 3:1.
In a further preferred embodiment, the solution comprising carbon source is the cellobiose solution of 10mg/L for concentration.
In a further preferred embodiment, comprise the solution of carbon source for the concentration cellobiose that is 12.5mg/L and sweet
The solution of the mixture of dew sugar, the weight of cellobiose and mannose ratio is for 1:1.
In a further preferred embodiment, comprise the solution of carbon source for concentration be 13.5mg/L sucrose and fiber two
The solution of the mixture of sugar, the weight of sucrose and cellobiose ratio is for 1:2.
In another preferred embodiment, the preparation method of microorganism formulation solution includes：By bacillus cereuss, photosynthetic
One or more of antibacterial, lactic acid bacteria ferment in nutrient substance and obtain.
One or more of above-mentioned three kinds of bacterium can be sent out in nutrient substance by the microorganism formulation solution of the present invention simultaneously
Ferment is obtained it is also possible to pass through fermenting above-mentioned three kinds of bacterium respectively for one or more, remixes and obtains.
In a further preferred embodiment, the fermentation temperature of microorganism formulation solution is 37 DEG C ± 1 DEG C.
In a further preferred embodiment, the preparation method of microorganism formulation solution includes：Stirring in sweat
Mix rotating speed 150～250rpm, ventilation is 1-2m3/ h, tank pressure 0.03～0.05Mpa.
In a further preferred embodiment, the preparation method of microorganism formulation solution includes：Fermentation time be 40～
60 hours.
In a further preferred embodiment, the preparation method of microorganism formulation solution includes：By the spore fermenting
Bacillus preparation, photosynthetic bacteria preparation, lactobacillus preparation are according to 3:1:1 volume ratio mixes, prepared microorganism formulation solution.
In the microorganism formulation of the present invention, the nutrient substance of fermentation is using conventional growth of microorganism energy substance (example
As molasses, milk, carbamide) and the mixture of trace element can realize.
In another preferred embodiment, microorganism formulation solution bacterial concentration is 1 × 1010More than cfu/ml.
In a further preferred embodiment, the preparation method of microorganism formulation solution includes：Fermentation can 50L～
Carry out in the fermentation tank of 500L, the liquid amount of fermentation is in about 60% (V/V).
In a preferred embodiment, the solution comprising carbon source is the watery or thick solution comprising carbon source,
For example comprise the aqueous solution of carbon source.
In a preferred embodiment, microorganism formulation solution is microorganism formulation aqueous solution or thick molten
Liquid, such as microorganism formulation aqueous solution.
In a preferred embodiment, ecology is processed using the solution comprising carbon source and/or microorganism formulation solution
Base is included using the solution comprising carbon source and/or microorganism formulation solution soaking and/or sprinkling and/or spraying ecological matrix.For example,
By ecological base section/be preferably all dipped into, in the solution that processes, (solution comprising carbon source and/or microorganism formulation are molten
Liquid) more than 10 minutes；Again for example, by the solution (solution comprising carbon source and/or microorganism formulation solution) being used for processing from
More than half an hour, all soaked by solution with ecological matrix and be advisable to lower or level sprinkling/spraying treatment ecological matrix.
In a preferred embodiment, it is dried and is advisable in the way of not using high temperature drying, can adopt excellent
The mode that choosing includes air-drying and/or drying etc. is dried, in order to avoid damaging the activity of the bacteria preparation of ecological matrix surface attachment.Example
As the ecological matrix after can processing solution air-dries or dries in its natural state.
In a further preferred embodiment, a kind of drying meanss preparing enhanced ecological base, including will adopt
With comprising the ecological matrix of the liquid handling of carbon source, natural air drying at be exposed to the sun under the sun drying, or shady and cool ventilation.
In a further preferred embodiment, a kind of drying meanss preparing enhanced ecological base, including will adopt
With comprising the ecological matrix of microorganism formulation solution process, dry at 40～55 DEG C, or natural air drying at shady and cool ventilation.
In a further preferred embodiment, a kind of method preparing enhanced ecological base, ecological matrix is soaked
More than 10 minutes in the sucrose solution of 10mg/L～40mg/L, then it is dried.
In a further preferred embodiment, a kind of method preparing enhanced ecological base, choose hay spore
As the single culture of strengthening, the preparation method of bacillus subtilis formulation solution is bacillus preparation：With energy substance (molasses,
Milk, a small amount of carbamide etc.) and trace element be bacillus subtilises nutrient substance, in the fermentation tank of such as 50L or 500L
Middle fermentation, is subsequently poured into Bacillus subtilis strain 500mL, 37 ± 1 DEG C of temperature control, speed of agitator 150～250rmp, filtrated air
Ventilation is 1～2m3/ h, tank pressure 0.03～0.05Mpa, harvest bacterium solution after natural fermentation 40～60h, bacterium solution is diluted to 1 ×
1010Cfu/ml bacillus subtilis formulation derived above solution, bacillus subtilis formulation solution using sprinkling and/or is sprayed
To on ecological matrix, all soaked by solution with ecological matrix and be advisable, the ecological matrix soaking is dried with the mode air-drying.
In a further preferred embodiment, a kind of method preparing enhanced ecological base, selection bacillus cereuss,
Photosynthetic bacteria, three kinds of strains of lactic acid bacteria, the preparation method of microorganism formulation solution is：(molasses, milk, a small amount of with energy substance
Carbamide etc.) and trace element be nutrient substance, then pour Bacillus subtilis strain, photosynthetic bacteria strain, lactic acid respectively into
Bacterium strain, 37 ± 1 DEG C of temperature control, speed of agitator 150～250rmp, filtrated air ventilation is 1～2m3/ h, tank pressure 0.03～
0.05Mpa, after natural fermentation 40～60h, you can harvest bacterium solution respectively.By the bacillus cereuss fermenting bacterium solution, photosynthetic bacteria bacterium
Liquid, lactic acid bacterial liquid are according to 3:1:1 volume ratio mixes, the concentration according to bacterium solution simultaneously, composite bacteria liquid is diluted to 1 ×
1010Cfu/ml microorganism formulation derived above solution, by microorganism formulation spray solution and/or be sprayed on ecological matrix, with life
State base is all soaked by solution and is advisable, and the ecological matrix soaking needs to be dried with the mode air-drying, and does not dry under high temperature, also not strong too
It is exposed to the sun under sunlight, be advisable with natural air drying at shady and cool ventilation.
2nd, enhanced ecological base
Ecological matrix is a kind of new bio carrier, can provide growth habitat for microorganisms in water, algae.Ecological matrix one
Denier is positioned in water, and, to its surface, As time goes on ecological primary surface can be attached to adsorb various aquatiles in water immediately
Growth microorganism and algae, these microorganisms and algae play the pass of biofiltration and biotransformation for eutrophication water
Key acts on.The microorganism being attached on ecological matrix is very abundant, mainly by antibacterial, funguses, algae, protozoacide and metazoa
Deng the ecosystem constituting complexity.Microorganism system passes through the Organic substance in the metabolism decomposition water of itself, raw on ecological matrix
Eutrophy composition in the long adsorbable water body of microorganism, the such as material such as nitrogen phosphate and sulfur, carbon, and these eutrophy compositions are rich
Collection, by different microbial actions, transforms into carbon dioxide, water and nitrogen etc., thus having captured needed for algal grown
Nutrient substance it is suppressed that the growing of algae, gradually improved by water quality.
The ecological matrix of the present invention can adopt any disclosed/known ecological matrix in current this area, can for example, wrap
Include the non-woven fabric-like body that chemical fibre silk and/or cordage are made, or the elastic brush of polypropylene and/or polyethylene composition
Body.
The enhanced ecological base of the present invention passes through to process using the solution comprising carbon source and/or microorganism formulation solution, raw
State primary surface adheres to substantial amounts of carbon source and/or microbial nutrition material, these materials as the attachment contributing to microorganisms in water,
Also provide nutrition and energy substance for microorganisms in water, accelerate growth and the breeding of microorganism, the raised growth of microorganism can divide
The Eutrophication materials of solution water body, and then purify water, the growth for Fish provides good environment, can promote Fish faster
Growth.
3rd, using method
The enhanced ecological base of the present invention can be used in breeding water body/cultivating system, for breeding water body/cultivating system
Process.For example in herbivorous fishes (Ctenopharyngodon idellus, Carassius auratuss etc.) breeding water body, in Chelonian (Testudiniss etc.) breeding water body, shrimps (right
Shrimp etc.) in breeding water body, compared with conventional ecological matrix, can be significantly reduced the biofilm time, lifting Water quality restoration efficiency, plus
The fast cultivation object speed of growth, reduction sickness rate.
The enhanced ecological base of the present invention, after preparing, can transport/preserve without affecting its effect.Make
Used time, the enhanced ecological base using method of the present invention is simple, identical with conventional ecological matrix using method/similar, for example, one
Individual preferred embodiment in, enhanced ecological base using include：Enhanced ecological base is placed in water body, with Coke bottle or
Empty oil drum is as float, and with nylon rope, ecological matrix two ends is fixed on pond two sides, and the distance between every piece of ecological matrix exists
Such as more than 2 meters, it is spaced alignment, the straight passage of shape, to reduce the obstruction to fish swimming simultaneously.4th, purposes
The present invention provides a kind of purposes in water process for enhanced ecological base.In water process, ecological matrix can be used for
The purification of water quality, improves water quality, particularly in landscape water body or breeding water body, has big economic worth.The present invention's
Enhanced ecological base, on the basis of retaining common ecological matrix effect/value, passes through further simply and easily to process, shortens it
The start-up time, improve startup stage water treatment efficiency, lifting pollutant removal speed is so that the performance of ecological matrix significantly carries
Height, to water treatment effect and economy/social value, has outstanding contributions.
Specifically, the biofilm time can be shortened 30%-50% by the enhanced ecological base of the present invention.
Specifically, Water quality restoration efficiency can be improved 10%-20% by the enhanced ecological base of the present invention.
Specifically, the enhanced ecological base of the present invention can make fish growth speed improve more than 10%, or even 20% with
On.
Specifically, the enhanced ecological base of the present invention can make disease incidence reduce more than more than 20%, or even 25%.
Based on above performance boost, the enhanced ecological base of the present invention has extensive use in water treatment field.
Based on above performance boost, the enhanced ecological base of the present invention has extensively in terms of the process/purification of breeding water body
General purposes.
5th, terminology used in the present invention is explained
As used in the present invention, " bacterial concentration " refers to the concentration of the thalline in microorganism formulation solution, its unit
Cfu/ml refers to the total number of bacterial colonies containing in every milliliter of microorganism formulation solution.
As used in the present invention, refer to comprise to be not more than using substantially free before something/certain class material
10%, or no more than 5%, or no more than 2%, or no more than 1%, or no more than 0.1% something/certain class material, or bag
Something containing 0%/certain class material.
As used in the present invention, " substantially only comprising " is used to refer to comprise to be not less than before something/certain class material
90%, or be not less than 95%, or be not less than 98%, or be not less than 99%, or about 100% something/certain class material.
As used in the present invention, " Water quality restoration efficiency " refers to work as using plant or microorganism to the pollutant in water body
Processed, the speed degree that the Eutrophication materials such as Water (N), phosphorus (P) are degraded/purified.
Below in conjunction with specific embodiment, explanation is further explained to the present invention.
Embodiment
Embodiment 1
The non-woven fabrics choosing the braiding of chemical fibre silk, as ecological matrix raw material, are cut into the size of 1m × 5m, system
It is made ecological sill.Sucrose is configured to according to the concentration of 20mg/L aqueous sucrose solution is standby, the non-woven fabrics cut out are given birth to
State base is immersed in more than 10 minutes in sucrose solution, then pulls drying out, and drying meanss using drying or are exposed to the sun or air-dried
Mode, during drying, temperature control is at 40～55 DEG C；It is exposed to the sun and is exposed to the sun one day under strong sunlight weather, till being dried；
Air-dry using at shady and cool ventilation natural air drying, air-dry time depending on weather condition, typically at least 2 days.Dried
The enhanced ecological base that ecological matrix completes.
Embodiment 2
Using the non-woven fabrics of chemical fibre silk braiding as ecological matrix raw material, it is cut into the size of 1m × 5m, make
Become ecological sill.Choose bacillus subtilis formulation as the single culture of strengthening, the system of bacillus subtilis formulation solution
Preparation Method is：Round selects the fermentation tank of 50L, with energy substance (molasses, milk, a small amount of carbamide etc.) and trace element
For bacillus subtilises nutrient substance, about liquid amount 60% (V/V), it is subsequently poured into Bacillus subtilis strain 500mL, temperature control
37 ± 1 DEG C, speed of agitator 200rmp, filtrated air ventilation is 1.5m3/ h, tank pressure 0.03～0.05Mpa, natural fermentation 40～
After 60h, you can harvest bacterium solution.According to the concentration harvesting bacterium solution during use, bacterium solution is diluted to 6 × 1010Cfu/ml～6 ×
1011The concentration of cfu/ml, bacillus subtilis formulation solution is using spraying and/or being sprayed on ecological matrix, whole with ecological matrix
Soaked by solution and be advisable, the ecological matrix soaking needs to be dried with the mode air-drying, can not be unable to dry under high temperature, also can not by force too
It is exposed to the sun under sunlight, be advisable with natural air drying at shady and cool ventilation.
Embodiment 3
Using the non-woven fabrics of chemical fibre silk braiding as ecological matrix raw material, it is cut into the size of 1m × 5m, make
Become ecological sill.Choose bacillus cereuss, photosynthetic bacteria, lactic acid bacteria culturerss, the preparation method of microorganism formulation solution is：First
Select 3 sets of 50L fermentation tanks as round, to Bacillus subtilis strain, photosynthetic bacteria strain, three kinds of bacterium of lactic acid bacteria culturerss
Agent carries out fermentation culture respectively, and 3 sets of systems are all with energy substance (molasses, milk, a small amount of carbamide etc.) and trace element for battalion
Foster material, about liquid amount 60% (V/V), then pours Bacillus subtilis strain, photosynthetic bacteria strain, lactic acid bacteria bacterium into respectively
Plant 500mL, 37 ± 1 DEG C of temperature control, speed of agitator 150～250rmp, filtrated air ventilation is 1.5m3/ h, tank pressure 0.03～
0.05Mpa, after natural fermentation 40～60h, you can harvest bacterium solution respectively, by the bacillus cereuss fermenting bacterium solution, photosynthetic bacteria bacterium
Liquid, lactic acid bacterial liquid are according to 3:1:1 volume ratio mixes, and the concentration according to composite bacteria liquid simultaneously, by composite bacteria liquid dilute with water
Become 5.0 × 1010Cfu/ml～5.0 × 1011The microorganism formulation solution of cfu/ml, by microorganism formulation spray solution and/or spray
Mist, on ecological matrix, is all soaked by solution with ecological matrix and is advisable, and the ecological matrix soaking needs to be dried with the mode air-drying, can not
It is unable to dry under high temperature, also can not be exposed to the sun under sunlight by force, be advisable with natural air drying at shady and cool ventilation.
Embodiment 4
The elastic brush body choosing polypropylene composition, as ecological matrix raw material, is woven into the biological hairbrush of 0.8m length, then
Hairbrush series connection is fabricated to the ecological sill of 0.8m × 5m.By sugarcane sugar and starch according to 3:1 weight ratio is configured to 22.5mg/
The aqueous solution of L, the brush made body ecological matrix is immersed in 30 minutes in sugarcane sugar and starch mixed solution, then pulls out in the moon
Cool ventilation natural air drying.
Application example 1
In Huizhou Huicheng District Zhenhua fishery company limited cultivation base, 2 mouthfuls of ponds, area 30 mu/mouthful, this year
Degree cultivates Ctenopharyngodon idellus, raises together with silver carp flathead, and yield, at 3500 kgs/acre, is put fingerling and placed the strengthening that embodiment 1 prepares after four months
Type ecological matrix.Through the cultivation of a culture-cycle, biofilm maturation time shortens 30-50%, and Water quality restoration efficiency improves
10%-20%, fish growth speed improves more than 10%, and disease incidence reduces by more than 20%, simultaneously sediment of pond deposition
Amount decreases 8%-17%, and quantity of exchanged water decreases more than 20%, achieves preferable economy and ecological benefits.
Application example 2
In Fushan City, Guangdong Province Nanhai District Tong Wei Aqua Sciences Inc. cultivation base, 4 mouthfuls of ponds, area 7 mu/mouthful,
Current year master supports Ctenopharyngodon idellus Collocation cultivation silver carp, flathead, and yield, at 3500-4000 kg/acre, is put fingerling and placed embodiment 3 after three months
The enhanced ecological base preparing, when certain culturing time reaches supermarket's grass carp listing specification in (about 10 months), strengthening
Type ecological matrix pond rate of body weight gain improves more than 25%, and ecological matrix pond significantly reduces water quality NO simultaneously2- N content is hence it is evident that control
Make the generation of the parasitic diseases such as lernaean, improved survival rate more than 15%, simultaneously ecological matrix Phytoplanktons group knot
Structure is changed based on chlorella, diatom and Euglena, and sediment of pond deposition decreases 20-30% simultaneously, and quantity of exchanged water decreases 15%
More than, mu increases economic efficiency more than 500 yuan, achieves preferable economy and ecological benefits.
The above, the only preferably specific embodiment of the present invention, but protection scope of the present invention is not limited thereto,
Any those familiar with the art the invention discloses technical scope in, technology according to the present invention scheme and its
Design in addition equivalent or change, all should cover within the scope of the present invention.
Claims (10)
1. a kind of preparation method of enhanced ecological base is it is characterised in that using the solution comprising carbon source and/or microorganism formulation
Solution processes ecological matrix, and strengthened after being dried type ecological matrix,
Wherein,
The described carbon source solution that comprises is the solution being substantially free of nitrogen source,
Described microorganism formulation is the preparation obtaining after one or more of bacillus cereuss, photosynthetic bacteria, lactic acid bacteria ferment.
2. the preparation method of enhanced ecological base according to claim 1 is it is characterised in that described solution is watery or viscous
Thick shape solution.
3. the preparation method of enhanced ecological base according to claim 1 is it is characterised in that the described solution comprising carbon source
For comprising the solution of the mixture of one or more of carbohydrate carbon source or biomass carbon source；
Wherein, described carbohydrate carbon source preferably is selected from the mixture of one or more of sucrose, glucose, starch；
Wherein, described biomass carbon source preferably is selected from the mixed of one or more of cellobiose, mannose, xylan, galactose
Compound.
4. the preparation method of enhanced ecological base according to claim 1 is it is characterised in that the described solution comprising carbon source
For substantially only comprising the solution of carbon source.
5. the preparation method of enhanced ecological base according to claim 1 is it is characterised in that described microorganism formulation solution
Preparation method include：One or more of bacillus cereuss, photosynthetic bacteria, lactic acid bacteria are fermented in nutrient substance and obtains；
The preferred bacterial concentration of described microorganism formulation solution is 1 × more than 1010cfu/ml.
6. the preparation method of enhanced ecological base according to claim 1 is it is characterised in that comprise the dense of the solution of carbon source
Degree scope is 10mg/L～40mg/L, preferably 20mg/L～25mg/L.
7. enhanced ecological base according to claim 1 preparation method it is characterised in that described process include soak,
Spray, spray.
8. enhanced ecological base according to claim 1 preparation method it is characterised in that described dry include air-drying,
Dry.
9. a kind of enhanced ecological base being prepared using the method described in any claim in claim 1-8.
10. a kind of enhanced ecological base according to claim 9 is it is characterised in that described enhanced ecological base is in cultivation
Biofilm maturation time in water body shortens 30-50%, and Water quality restoration efficiency improves 10%-20%, and fish growth speed improves
More than 10%, disease incidence reduces by more than 20%.
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| CN116693067A (en) * | 2023-07-14 | 2023-09-05 | 中国水产科学研究院珠江水产研究所 | In-situ restoration method for jointly treating cultivation tail water by utilizing duckweed-loaded plant growth promoting bacteria |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105883999A (en) * | 2016-04-26 | 2016-08-24 | 大连汇新钛设备开发有限公司 | Calcium oxide sustained-release block for regulating pH value of recirculating aquaculture system and preparation method of calcium oxide sustained-release block for regulating pH value of recirculating aquaculture system |
| CN108541638A (en) * | 2018-04-19 | 2018-09-18 | 广州普麟生物制品有限公司 | A kind of Penaeus Vannmei pond outdoor breeding method |
| CN116693067A (en) * | 2023-07-14 | 2023-09-05 | 中国水产科学研究院珠江水产研究所 | In-situ restoration method for jointly treating cultivation tail water by utilizing duckweed-loaded plant growth promoting bacteria |
| CN116693067B (en) * | 2023-07-14 | 2024-02-06 | 中国水产科学研究院珠江水产研究所 | In-situ restoration method for jointly treating cultivation tail water by utilizing duckweed-loaded plant growth promoting bacteria |
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