CN109762723B - Device for photosynthetic bacteria culture - Google Patents

Abstract

The invention discloses a device for cultivating photosynthetic bacteria, comprising a device body, wherein a feed port is penetrated through the top of the device body, a feed port cover is connected to the top side of the feed port through a movable shaft, and a handle is fixed on the top of the feed port cover, an observation window is arranged above the outer surface of the device body, an operating table is arranged below the outer surface of the device body, and a purification chamber is arranged inside the device body, a user can turn on an ultraviolet sterilizer through the operating table to sterilize the culture medium in the purification chamber to ensure the purity of the culture medium to be processed, the sterilized culture medium enters the culture chamber through an impurity filter net to ensure the purity of the culture medium; the photosynthetic bacteria to be cultured are filtered, introduced into a liquid storage tank from a bacterial liquid port, and evenly sprayed into the culture medium in the culture chamber by a nozzle; stirring is performed at a constant temperature, and pure photosynthetic bacterial microorganisms grown are output from a discharge port at the bottom of the device.

Description

A device for cultivating photosynthetic bacteria

Technical Field

The invention relates to the technical field of microbial propagation, in particular to a device for culturing photosynthetic bacteria.

Background technique

Photosynthetic bacteria is a kind of probiotics, which plays an important role in the carbon, nitrogen and sulfur conversion cycle in nature. It is rich in protein, vitamins and a variety of biologically active ingredients and physiologically active substances, and has the functions of photosynthesis, fermentation, nitrogen fixation and hydrogen production. Therefore, photosynthetic bacteria have high application value in environmental protection, agriculture, medicine, aquatic products and livestock and poultry farming. At present, photosynthetic bacteria are mainly cultivated artificially, and there are many factors affecting the cultivation of photosynthetic bacteria, such as strain type, culture medium, culture conditions, etc. In the existing photosynthetic bacteria cultivation process, culture medium is usually required. Because the culture medium contains different nutrients, these substances are inevitably mixed with other bacteria due to low purity and different storage methods, which will affect the overall cultivation quality of photosynthetic bacteria microorganisms. It is necessary to sterilize the culture medium in advance to remove the bacteria. The existing sterilization method generally adopts high temperature and high pressure sterilization means, which is easy to cause danger, and the sterilization method needs to be improved; in addition, when the existing photosynthetic bacteria microorganisms are cultivated, most of them are simply stirred and mixed with photosynthetic bacteria strains and culture medium, so that the efficiency of photosynthetic bacteria absorbing culture medium is not high, resulting in too long time for cultivation.

Summary of the invention

The object of the present invention is to provide a device for culturing photosynthetic bacteria to solve the problems mentioned in the above background technology that the purity of the culture medium is not high and it contains other miscellaneous bacteria, which affects the overall cultivation quality of microorganisms; and that simple stirring and mixing of the culture medium and photosynthetic bacteria will lead to low efficiency of photosynthetic bacteria absorbing the culture medium and prolonged cultivation time.

To achieve the above-mentioned purpose, the present invention provides the following technical solutions: a device for culturing photosynthetic bacteria, comprising a device body, a feed inlet is penetrated at the top of the device body, a feed inlet cover is connected to one side of the top of the feed inlet through a movable shaft, and a handle is fixed to the top of the feed inlet cover, an observation window is arranged above the outer surface of the device body, and an operating table is arranged below the outer surface of the device body; a liquid storage tank is arranged at one side of the device body, and a bacterial liquid port is penetrated at one side of the liquid storage tank, a cultivation bin is connected at the bottom of the device body, a discharge port is penetrated below the outer surface of the cultivation bin, and a support leg is connected to the bottom of the cultivation bin; a machine box is fixed in the middle of the bottom of the cultivation bin, a purification chamber is arranged inside the device body, an ultraviolet sterilizer is arranged on one side of the purification chamber, and a connecting pipe is connected to the bottom of the purification chamber; a liquid storage chamber is arranged inside the liquid storage tank, and a nozzle is penetrated at the bottom of the liquid storage chamber, a cultivation chamber is arranged inside the cultivation bin, a thermostat is arranged inside the cultivation chamber, a motor is arranged inside the machine box, and a stirring rod is connected to the output end of the motor.

Preferably, a filter screen is disposed inside the bacteria liquid port, and the cross-sectional area of the filter screen is equal to the cross-sectional area of the inside of the bacteria liquid port.

Preferably, an impurity filter is provided at the lower part of the purification chamber.

Preferably, the stirring rod is transversely arranged at the lower part of the cultivation chamber, and the bottom of the stirring rod passes through the bottom of the cultivation chamber and is connected to the output end of the motor.

Preferably, the cross-sectional area of the impurity filter screen is equal to the cross-sectional area of the connecting pipe, and the impurity filter screen is connected to the connecting pipe in a sleeve-type manner.

Preferably, two thermostats are provided, and the two thermostats are at the same level.

Compared with the prior art, the invention has the following beneficial effects: the device for culturing photosynthetic bacteria is provided with an ultraviolet sterilizer and an impurity filter net; a user introduces a culture medium into a purification chamber through a feed inlet, turns on the ultraviolet sterilizer to quickly kill the miscellaneous bacteria in the culture medium; the culture medium after killing the miscellaneous bacteria passes through the impurity filter net to filter out suspended matter and other impurities in the culture medium, thereby ensuring the purity of the culture medium, thereby solving the problem of low purity of the culture medium; the culture medium killed by ultraviolet rays is introduced into the culture chamber through a connecting pipe to wait for photosynthetic bacteria strains to be added; the photosynthetic bacteria strains required to be cultured are introduced into the liquid storage tank through the filter net of the bacterial liquid port; in the device, a nozzle is penetrated through the bottom of the liquid storage chamber, which can convert the strains into smaller forms and evenly disperse them in the culture medium, thereby facilitating the growth of the microbial community, thereby solving the problem that the photosynthetic bacteria absorb the culture medium inefficiently and the required cultivation time is prolonged due to the simple stirring and mixing of the culture medium and the photosynthetic bacteria directly.

BRIEF DESCRIPTION OF THE DRAWINGS

Fig. 1 is a schematic diagram of the structure of the present invention;

FIG2 is a schematic diagram of the internal structure of the main body of the device of the present invention;

FIG. 3 is a schematic diagram of a partially enlarged structure of A of the present invention.

In the figure: 1. device body; 2. feed inlet; 3. feed inlet cover; 4. handle; 5. movable shaft; 6. observation window; 7. operating table; 8. liquid storage tank; 9. bacterial liquid outlet; 10. filter screen; 11. cultivation chamber; 12. discharge port; 13. chassis; 14. support foot; 15. ultraviolet sterilizer; 16. purification chamber; 17. impurity filter screen; 18. liquid storage chamber; 19. nozzle; 20. connecting pipe; 21. thermostat; 22. motor; 23. stirring rod; 24. cultivation chamber.

Detailed ways

The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

In the present invention, the thermostat model is ATS-MH-2800A/D/E, and the ultraviolet sterilizer model is SYZW150-24.

Referring to Figures 1-3, the present invention provides a technical solution: a device for culturing photosynthetic bacteria, comprising a device body 1, a feed port 2, a feed port cover 3, a handle 4, a movable shaft 5, an observation window 6, an operating table 7, a liquid storage tank 8, a bacterial liquid port 9, a filter screen 10, a cultivation bin 11, a discharge port 12, a chassis 13, a support leg 14, an ultraviolet sterilizer 15, a purification chamber 16, an impurity filter screen 17, a liquid storage chamber 18, a nozzle 19, a connecting pipe 20, a thermostat 21, a motor 22, a stirring rod 23 and a cultivation chamber 24, wherein the top of the device body 1 is penetrated by a feed port 2, a top side of the feed port 2 is connected to the feed port cover 3 through a movable shaft 5, and a handle 4 is fixed to the top of the feed port cover 3 An observation window 6 is arranged above the outer surface of the device body 1, an operating table 7 is arranged below the outer surface of the device body 1, a liquid storage tank 8 is arranged on one side of the device body 1, and a bacterial liquid port 9 is penetrated through one side of the liquid storage tank 8, a filter screen 10 is arranged inside the bacterial liquid port 9, and the cross-sectional area of the filter screen 10 is equal to the cross-sectional area inside the bacterial liquid port 9, so that the cultured microorganisms can be filtered more comprehensively during operation; a cultivation bin 11 is connected to the bottom of the device body 1, a discharge port 12 is penetrated below the outer surface of the cultivation bin 11, a support leg 14 is connected to the bottom of the cultivation bin 11, and a chassis 13 is fixed in the middle of the bottom of the cultivation bin 11, and the device The main body 1 is provided with a purification chamber 16 inside, and an impurity filter 17 is provided at the lower part of the purification chamber 16. When working, the culture solution inside the purification chamber 16 can be filtered. The cross-sectional area of the impurity filter 17 is equal to the cross-sectional area of the connecting pipe 20. A control valve is provided on the upper part of the connecting pipe 20, and the impurity filter 17 is connected to the upper end of the connecting pipe 20 in the form of a sleeve, which can increase the efficiency of the work when working. An ultraviolet sterilizer 15 is provided on one side of the purification chamber 16, and the bottom of the purification chamber 16 is connected to the connecting pipe 20, and the upper part of the connecting pipe 20 is provided with a control valve; the liquid storage tank 8 is provided with a liquid storage chamber 1 8, and a nozzle 19 is penetrated at the bottom of the liquid storage chamber 18, a cultivation chamber 24 is arranged inside the cultivation warehouse 11, and a thermostat 21 is arranged inside the cultivation chamber 24, and two thermostats 21 are arranged, and the two thermostats 21 are at the same horizontal height, which can better provide the required temperature for the photosynthetic bacteria in the cultivation chamber 24 when working, a motor 22 is arranged inside the chassis 13, and the output end of the motor 22 is connected to a stirring rod 23, the stirring rod 23 is horizontally arranged at the bottom of the cultivation chamber 24, and the bottom of the stirring rod 23 passes through the bottom of the cultivation chamber 24 and is connected to the output end of the motor 22, which can increase the work efficiency when working.

The impurity filter 17 is connected to the upper end of the connecting pipe 20 in a sleeve-type manner. When too much debris is trapped on the surface of the impurity filter 17, the material port cover 3 is opened, the impurity filter 17 is taken out, rinsed with water, and placed in the device, and then the ultraviolet sterilizer 15 is turned on to sterilize its surface for recycling.

Working principle: First, before using the device, check whether the performance of each component is normal. After checking that the performance of each component is normal, place the device at the designated work location. After placing the device, turn on the power supply. The user opens the feed inlet cover 3 through the handle 4, closes the valve on the connecting pipe 20, and introduces the required culture solution into the purification chamber 16 through the feed inlet 2. The user can turn on the ultraviolet sterilizer 15 through the operating table 7 to quickly kill the miscellaneous bacteria in the culture medium, and enter the cultivation chamber 24 through the impurity filter 17 through the connecting pipe 20 (open the control valve), waiting for the photosynthetic bacteria to be introduced. The above operation filters the required culture medium through the impurity filter 17, purifies and removes impurities from the culture medium again, and ensures the purity of the culture medium; after the above culture medium is free of bacteria, the ultraviolet sterilizer 15 is closed, the control valve on the upper part of the connecting pipe 20 is opened, and the culture medium after the bacteria are killed is introduced into the cultivation chamber 24 through the connecting pipe 20, and at the same time, the required photosynthetic bacteria strains are introduced into the liquid storage chamber 18 inside the liquid storage tank 8 through the bacteria liquid port 9, and then the photosynthetic bacteria strains are sprayed into the culture medium by the nozzle 19 penetrating the bottom of the liquid storage chamber 18, and then, the motor 22 is started through the operating table 7, and the motor 22 drives the stirring rod 23 to stir the photosynthetic bacteria inside the cultivation chamber 24 to promote the absorption of the culture medium.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims (3)

1. A device for cultivating photosynthetic bacteria, comprising a device body (1), characterized in that: a feed inlet (2) is penetrated at the top of the device body (1), a feed inlet cover (3) is connected to the top side of the feed inlet (2) through a movable shaft (5), and a handle (4) is fixed to the top of the feed inlet cover (3), an observation window (6) is arranged above the outer surface of the device body (1), an operating table (7) is arranged below the outer surface of the device body (1), a liquid storage tank (8) is arranged on one side of the device body (1), and a bacterial liquid port (9) is penetrated on one side of the liquid storage tank (8), a cultivation chamber (11) is connected to the bottom of the device body (1), a discharge port (12) is penetrated below the outer surface of the cultivation chamber (11), and the bottom of the cultivation chamber (11) is A support leg (14) is connected, a cabinet (13) is fixed in the middle of the bottom of the cultivation chamber (11), a purification chamber (16) is arranged inside the main body (1), an ultraviolet sterilizer (15) is arranged on one side of the interior of the purification chamber (16), a connecting pipe (20) is connected to the bottom of the purification chamber (16), and a control valve is arranged on the upper part of the connecting pipe (20); a liquid storage chamber (18) is arranged inside the liquid storage box (8), and a nozzle (19) penetrates the bottom of the liquid storage chamber (18); a cultivation chamber (24) is arranged inside the cultivation chamber (11), and a thermostat (21) is arranged inside the cultivation chamber (24); a motor (22) is arranged inside the cabinet (13), and a stirring rod (23) is connected to the output end of the motor (22); Wherein, a filter screen (10) is arranged inside the bacteria liquid port (9), and the cross-sectional area of the filter screen (10) is equal to the cross-sectional area inside the bacteria liquid port (9); Wherein, an impurity filter screen (17) is provided at the lower part of the purification chamber (16); The stirring rod (23) is disposed transversely at the bottom of the cultivation chamber (24), and the bottom of the stirring rod (23) passes through the bottom of the cultivation chamber (24) and is connected to the output end of the motor (22). 2. A device for cultivating photosynthetic bacteria according to claim 1, characterized in that the cross-sectional area of the impurity filter (17) is equal to the cross-sectional area of the connecting pipe (20), and the impurity filter (17) is connected to the connecting pipe (20) in a sleeve-type form. 3. The device for culturing photosynthetic bacteria according to claim 1, characterized in that: two thermostats (21) are provided, and the two thermostats (21) are at the same level.